CN1553175A - Plastic chip for electrochemical test and preparing method thereof - Google Patents
Plastic chip for electrochemical test and preparing method thereof Download PDFInfo
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- CN1553175A CN1553175A CNA03133508XA CN03133508A CN1553175A CN 1553175 A CN1553175 A CN 1553175A CN A03133508X A CNA03133508X A CN A03133508XA CN 03133508 A CN03133508 A CN 03133508A CN 1553175 A CN1553175 A CN 1553175A
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- chip
- electrochemical detection
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- plastic chip
- pcb board
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Abstract
A plastic chip is made by sticking chip formed with two layers of PMMA board to a layer of PCB board as operation electrode is set on chip, high voltage electrode, electrochemical detection electrode or named as reference electrode, counter electrode and high voltage earth electrode are set on PCB board; and electrochemical detection pool is made on tail end of separation channel.
Description
Technical field:
The present invention relates to Electrochemical Detection chip and preparation method thereof.
Background technology:
In chip electrophoresis, laser-induced fluorescence (LIF) is because highly sensitive, and becomes the detection mode of main flow.But the necessary LASER Light Source of laser-Induced Fluorescence Detection mode, peaceful moving platform etc. all need further reduced volume, follow the volume of chip to adapt.Electrochemical Detection also is widely used in the detection of chip electrophoresis as detection mode commonly used in the Capillary Electrophoresis.In the Electrochemical Detection mode, the making of microelectrode can because Electrochemical Detection is based on the redox reaction of material at electrode surface, and follow light path irrelevant with the relevant technology manufacturing of chip manufacturing, and microminiaturization can not reduced sensitivity.And the electrochemical detector cost is low, is easy to microminiaturization, so the Electrochemical Detection mode is that the easiest small volume with chip adapts, thereby realizes one of microminiaturized detection mode.
1998, mathies has reported since the article of first piece of writing chip electrophoresis Electrochemical Detection, Electrochemical Detection has been subjected to paying attention to widely, material from chip, the manufacturing of electrode, all carried out broad research to application of Electrochemical Detection etc., for the application of chip electrophoresis Electrochemical Detection lays a solid foundation.But the Electrochemical Detection chip except high-field electrode, the contrast electrode that also has Electrochemical Detection to use, working electrode, to electrode etc., this has brought inconvenience to Electrochemical Detection.For this reason latest developments a kind of complete or collected works' accepted way of doing sth Electrochemical Detection chip, with the method for photoetching the Pt electrode is integrated on the glass-chip, made things convenient for operation.But because adopted noble metal etc. to make costing an arm and a leg of this chip, be unfavorable for the promotion and application of this chip, and concerning the galvanochemistry chip, electrode is contaminated easily, lost efficacy.So the integrative electrochemical of developing low-cost detects the application that will promote Electrochemical Detection with chip.
We design and have made plastics Electrochemical Detection chip cheaply for this reason.
Summary of the invention:
The invention provides a kind of Electrochemical Detection plastic chip, chip (1,2) and one deck pcb board (3) applying of being made by two-layer PMMA sheet form, be shaped on working electrode (4) on the chip, be shaped on contrast electrode that high-field electrode (5), Electrochemical Detection use on the pcb board (3) and electrode (6) and high pressure ground electrode (7); Be shaped on electrochemical investigating pond (8) in tail end of separation channel.
Electrochemical Detection provided by the invention is with in the plastic chip, and described working electrode carbon fiber (4) is to link to each other with lead (9) with conducting resinl, and then is connected with working electrode terminals (10).
Electrochemical Detection provided by the invention is with in the plastic chip, and described working electrode (4) is that diameter is the carbon fiber of 6 μ m.
Electrochemical Detection provided by the invention is with in the plastic chip, and described pcb board (3) is an Industrial PC B coated plate.
Electrochemical Detection provided by the invention comprises the preparation of PMMA chip with the preparation method of plastic chip, the preparation of pcb board and with the applying of PMMA sheet, carbon fiber is connected with electrode with conducting resinl.
Electrochemical Detection provided by the invention is with among the preparation method of plastic chip, and two-layer PMMA sheet is fitted with hot-press method.
Electrochemical Detection provided by the invention is with among the preparation method of plastic chip, and the PMMA sheet is adhered on the pcb board with PDMS.
Electrochemical Detection provided by the invention will polish with abrasive paper for metallograph away from an end of pressure hole after the pressing of two-layer PMMA sheet with among the preparation method of plastic chip.
Electrochemical Detection provided by the invention is easy to manufacture, with low cost with plastic chip.
Description of drawings:
Fig. 1 is the structural representation of Electrochemical Detection of the present invention with plastic chip;
Fig. 2 is the PMMA chip synoptic diagram of Electrochemical Detection of the present invention with plastic chip;
To be Electrochemical Detection of the present invention close the sheet synoptic diagram with the PMMA that has the carbon fiber working electrode of plastic chip to Fig. 3;
Fig. 4 is the pcb board synoptic diagram of Electrochemical Detection of the present invention with plastic chip.
Embodiment:
Making step is as follows:
One, makes the silicon chip template that needs with the method for photoetching.
Two, the method with hot pressing is fabricated into (as shown in Figure 2) on the PMMA sheet with passage, in the punching of corresponding position, to be used for applying high pressure.
Three, the punching of the relevant position on another sheet PMMA sheet.
Four,, adopt the method for hot pressing to be sealed to together with two PMMA sheets.
Five, will polish with abrasive paper for metallograph away from an end of pressure hole.
Six, the carbon fiber of diameter 6 μ m is bonded at the exit of split tunnel, the careful central authorities' (as shown in Figure 3) that carbon fiber placed outlet with epoxy glue.With conducting resinl carbon fiber is drawn, connect lead.Conducting resinl is coated with the last layer epoxy glue.
Seven, adopt industrialization PCB coated plate production technology to produce to have high-field electrode and contrast electrode and to the pcb board (as shown in Figure 4) of electrode.
Eight, on pcb board, be coated with one deck silica gel after, with PMMA chip stick on top (as shown in Figure 1), note not being coated on the electrode; Place baking oven 70 degree, 1 hour.
Nine, at an end of split tunnel, make electrochemical investigating pond.
Claims (8)
1, a kind of Electrochemical Detection plastic chip, it is characterized in that chip (1,2) and one deck pcb board (3) applying of being made by two-layer PMMA sheet form, be shaped on working electrode (4) on the chip, be shaped on high-field electrode (5), Electrochemical Detection electrode (6) and high pressure ground electrode (7) on the pcb board (3).
2,, it is characterized in that described working electrode (4) is to link to each other with lead (9) with conducting resinl according to the described Electrochemical Detection plastic chip of claim 1.
3,, it is characterized in that described working electrode (4) is that diameter is the carbon fiber of 6 μ m according to the described Electrochemical Detection plastic chip of claim 2.
4, according to the described Electrochemical Detection plastic chip of claim 1, described pcb board (3) is an Industrial PC B coated plate.
5, the described Electrochemical Detection of claim 1 preparation method of plastic chip is characterized in that comprising:
1) preparation of PM MA chip,
2) system of pcb board.Be equipped with and with the applying of PMMA sheet,
3) carbon fiber is connected with electrode with conducting resinl.
6,, it is characterized in that two-layer PMMA sheet fits with hot-press method according to the preparation method of the described Electrochemical Detection of claim 5 with plastic chip.
7, according to the preparation method of the described Electrochemical Detection of claim 5, it is characterized in that the PMMA chip is adhered on the pcb board with PDMS with plastic chip.
8, according to the preparation method of the described Electrochemical Detection of claim 5, it is characterized in that after the pressing of two-layer PMMA sheet, will polishing with abrasive paper for metallograph away from an end of pressure hole with plastic chip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03133508 CN1242258C (en) | 2003-05-28 | 2003-05-28 | Plastic chip for electrochemical test and preparing method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03133508 CN1242258C (en) | 2003-05-28 | 2003-05-28 | Plastic chip for electrochemical test and preparing method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1553175A true CN1553175A (en) | 2004-12-08 |
| CN1242258C CN1242258C (en) | 2006-02-15 |
Family
ID=34323058
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03133508 Expired - Fee Related CN1242258C (en) | 2003-05-28 | 2003-05-28 | Plastic chip for electrochemical test and preparing method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1242258C (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100454016C (en) * | 2007-01-05 | 2009-01-21 | 福州大学 | Thin-layer spectral electrochemical investigating pond |
| CN100458436C (en) * | 2007-02-14 | 2009-02-04 | 沈阳银捷机械装备制造有限责任公司 | Multifunction electromagnetic flaw detection device |
| CN102549626A (en) * | 2009-08-10 | 2012-07-04 | 苹果公司 | Mechanisms for detecting tampering of an electronic device |
-
2003
- 2003-05-28 CN CN 03133508 patent/CN1242258C/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100454016C (en) * | 2007-01-05 | 2009-01-21 | 福州大学 | Thin-layer spectral electrochemical investigating pond |
| CN100458436C (en) * | 2007-02-14 | 2009-02-04 | 沈阳银捷机械装备制造有限责任公司 | Multifunction electromagnetic flaw detection device |
| CN102549626A (en) * | 2009-08-10 | 2012-07-04 | 苹果公司 | Mechanisms for detecting tampering of an electronic device |
| CN102549626B (en) * | 2009-08-10 | 2014-03-12 | 苹果公司 | Mechanisms for detecting tampering of electronic device |
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| Publication number | Publication date |
|---|---|
| CN1242258C (en) | 2006-02-15 |
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