CN2615665Y

CN2615665Y - Plastic chip for electrochemical detection

Info

Publication number: CN2615665Y
Application number: CN 03213379
Authority: CN
Inventors: 姜雷; 秦建华; 刘兆奕; 戴忠鹏; 林炳承
Original assignee: Dalian Institute of Chemical Physics of CAS
Current assignee: Dalian Institute of Chemical Physics of CAS
Priority date: 2003-05-28
Filing date: 2003-05-28
Publication date: 2004-05-12
Anticipated expiration: 2013-05-28

Abstract

The utility model provides a plastic chip for electrochemical detection. The utility model comprises a chip made of two layers of PMMA pieces and a layer of PCB plate. The two layers of PMMA pieces and the layer of PCB plate are jointed together. A working electrode is equipped on the chip. The carbon fiber of the working electrode is connected with a conductor wire by conducting resin, and then is connected with the terminal of the working electrode. A high voltage electrode, an electrode for electrochemical detection or named by reference electrode, a counter electrode and a high voltage earth electrode are equipped on the PCB plate. An electrochemical detection cell is equipped on the end of the separating channel. The plastic chip for electrochemical detection provided by the utility model is easy to produce and low in cost.

Description

A kind of Electrochemical Detection plastic chip

Technical field:

The utility model relates to Electrochemical Detection chip and preparation method thereof.

Background technology:

In chip electrophoresis, laser-induced fluorescence (LIF) is because highly sensitive, and becomes the detection mode of main flow.But the necessary LASER Light Source of laser-Induced Fluorescence Detection mode, peaceful moving platform etc. all need further reduced volume, follow the volume of chip to adapt.Electrochemical Detection also is widely used in the detection of chip electrophoresis as detection mode commonly used in the Capillary Electrophoresis.In the Electrochemical Detection mode, the making of microelectrode can because Electrochemical Detection is based on the redox reaction of material at electrode surface, and follow light path irrelevant with the relevant technology manufacturing of chip manufacturing, and microminiaturization can not reduced sensitivity.And the electrochemical detector cost is low, is easy to microminiaturization, so the Electrochemical Detection mode is that the easiest small volume with chip adapts, thereby realizes one of microminiaturized detection mode.

1998, mathies has reported since the article of first piece of writing chip electrophoresis Electrochemical Detection, Electrochemical Detection has been subjected to paying attention to widely, material from chip, the manufacturing of electrode, all carried out broad research to application of Electrochemical Detection etc., for the application of chip electrophoresis Electrochemical Detection lays a solid foundation.But the Electrochemical Detection chip except high-field electrode, the contrast electrode that also has Electrochemical Detection to use, working electrode, to electrode etc., this has brought inconvenience to Electrochemical Detection.For this reason latest developments a kind of complete or collected works' accepted way of doing sth Electrochemical Detection chip, with the method for photoetching the Pt electrode is integrated on the glass-chip, made things convenient for operation.But because adopted noble metal etc. to make costing an arm and a leg of this chip, be unfavorable for the promotion and application of this chip, and concerning the galvanochemistry chip, electrode is contaminated easily, lost efficacy.So the integrative electrochemical of developing low-cost detects the application that will promote Electrochemical Detection with chip.

We design and have made plastics Electrochemical Detection chip cheaply for this reason.

Summary of the invention:

The utility model provides a kind of Electrochemical Detection plastic chip, chip (1,2) and one deck pcb board (3) applying of being made by two-layer PMMA sheet form, be shaped on working electrode (4) on the chip, be shaped on contrast electrode that high-field electrode (5), Electrochemical Detection use on the pcb board (3) and electrode (6) and high pressure ground electrode (7); Be shaped on electrochemical investigating pond (8) in tail end of separation channel.

The Electrochemical Detection that the utility model provides is with in the plastic chip, and described working electrode-carbon fiber (4) is to link to each other with lead (9) with conducting resinl, and then is connected with working electrode terminals (10).

The Electrochemical Detection that the utility model provides is with in the plastic chip, and described working electrode (4) is that diameter is the carbon fiber of 6 μ m.

The Electrochemical Detection that the utility model provides is with in the plastic chip, and described pcb board (3) is an Industrial PC B coated plate.

The Electrochemical Detection that the utility model provides is easy to manufacture, with low cost with plastic chip.

Description of drawings:

Fig. 1 is the structural representation of Electrochemical Detection of the present utility model with plastic chip;

Fig. 2 is the PMMA chip synoptic diagram of Electrochemical Detection of the present utility model with plastic chip;

To be Electrochemical Detection of the present utility model close the sheet synoptic diagram with the carbon fiber working electrode PMMA that has of plastic chip to Fig. 3;

Fig. 4 is the pcb board synoptic diagram of Electrochemical Detection of the present utility model with plastic chip.

Embodiment:

Making step is as follows:

One, makes the silicon chip template that needs with the method for photoetching.

Two, the method with hot pressing is fabricated into (as shown in Figure 2) on the PMMA sheet with passage, in the punching of the position of phase, to be used for applying high pressure.

Three, the punching of the relevant position on another sheet PMMA sheet.

Four,, adopt the method for hot pressing to be sealed to together with two PMMA sheets.

Five, will polish with abrasive paper for metallograph away from an end of pressure hole.

Six, the carbon fiber (4) of diameter 6 μ m is bonded at the exit of split tunnel, the careful central authorities' (as shown in Figure 3) that carbon fiber (4) placed outlet with epoxy glue.With conducting resinl carbon fiber (4) is drawn, connect lead.Conducting resinl is coated with the last layer epoxy glue.

Seven, adopt industrialization PCB coated plate production technology to produce to have high-field electrode (5) and contrast electrode and to the pcb board (3) (as shown in Figure 4) of electrode (6).

Eight, on pcb board (3), be coated with one deck PDMS after, with PMMA chip stick on top (as shown in Figure 1), note not being coated on the electrode; Place baking oven 70 degree, 1 hour.

Nine, at an end of split tunnel, make electrochemical investigating pond.

Claims

1, a kind of Electrochemical Detection plastic chip, it is characterized in that chip (1,2) and one deck pcb board (3) applying of being made by two-layer PMMA sheet form, be shaped on working electrode (4) on the chip, be shaped on high-field electrode (5), Electrochemical Detection electrode (6) and high pressure ground electrode (7) on the pcb board (3).

2,, it is characterized in that described working electrode (4) is to link to each other with lead (9) with conducting resinl according to the described Electrochemical Detection plastic chip of claim 1.

3,, it is characterized in that described working electrode (4) is that diameter is the carbon fiber of 6 μ m according to the described Electrochemical Detection plastic chip of claim 2.

4, according to the described Electrochemical Detection plastic chip of claim 1, described pcb board (3) is an Industrial PC B coated plate.