CN1525165A - 一种金属螯合层析填料的制备方法 - Google Patents
一种金属螯合层析填料的制备方法 Download PDFInfo
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- CN1525165A CN1525165A CNA031155162A CN03115516A CN1525165A CN 1525165 A CN1525165 A CN 1525165A CN A031155162 A CNA031155162 A CN A031155162A CN 03115516 A CN03115516 A CN 03115516A CN 1525165 A CN1525165 A CN 1525165A
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- iminodiacetic acid
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- 229910052751 metal Inorganic materials 0.000 title claims abstract description 18
- 239000002184 metal Substances 0.000 title claims abstract description 18
- 238000004587 chromatography analysis Methods 0.000 title claims abstract description 15
- 238000002360 preparation method Methods 0.000 title claims description 7
- 230000009920 chelation Effects 0.000 title 1
- NBZBKCUXIYYUSX-UHFFFAOYSA-N iminodiacetic acid Chemical compound OC(=O)CNCC(O)=O NBZBKCUXIYYUSX-UHFFFAOYSA-N 0.000 claims abstract description 15
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 24
- 239000000945 filler Substances 0.000 claims description 24
- 239000013522 chelant Substances 0.000 claims description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 229920002684 Sepharose Polymers 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 10
- 239000012153 distilled water Substances 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 10
- 238000006243 chemical reaction Methods 0.000 claims description 9
- 229910000033 sodium borohydride Inorganic materials 0.000 claims description 9
- 239000012279 sodium borohydride Substances 0.000 claims description 9
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims description 5
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 5
- 238000010792 warming Methods 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 230000007717 exclusion Effects 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 4
- 238000001742 protein purification Methods 0.000 abstract description 4
- 239000011543 agarose gel Substances 0.000 abstract 1
- 239000011159 matrix material Substances 0.000 description 10
- 102000004169 proteins and genes Human genes 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- 230000004913 activation Effects 0.000 description 5
- 238000001994 activation Methods 0.000 description 5
- 239000007795 chemical reaction product Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 229920000936 Agarose Polymers 0.000 description 3
- 229920005654 Sephadex Polymers 0.000 description 3
- 239000012507 Sephadex™ Substances 0.000 description 3
- 102000002933 Thioredoxin Human genes 0.000 description 3
- 150000002460 imidazoles Chemical class 0.000 description 3
- 108060008226 thioredoxin Proteins 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 2
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 2
- 102000019197 Superoxide Dismutase Human genes 0.000 description 2
- 108010012715 Superoxide dismutase Proteins 0.000 description 2
- 238000001042 affinity chromatography Methods 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 238000011010 flushing procedure Methods 0.000 description 2
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- LGQLOGILCSXPEA-UHFFFAOYSA-L nickel sulfate Chemical compound [Ni+2].[O-]S([O-])(=O)=O LGQLOGILCSXPEA-UHFFFAOYSA-L 0.000 description 2
- 229910000363 nickel(II) sulfate Inorganic materials 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000012048 reactive intermediate Substances 0.000 description 2
- 229940094937 thioredoxin Drugs 0.000 description 2
- WFIYPADYPQQLNN-UHFFFAOYSA-N 2-[2-(4-bromopyrazol-1-yl)ethyl]isoindole-1,3-dione Chemical compound C1=C(Br)C=NN1CCN1C(=O)C2=CC=CC=C2C1=O WFIYPADYPQQLNN-UHFFFAOYSA-N 0.000 description 1
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 1
- -1 IDA metalloid Chemical class 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 239000006035 Tryptophane Substances 0.000 description 1
- PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 239000010836 blood and blood product Substances 0.000 description 1
- 229940125691 blood product Drugs 0.000 description 1
- 229910001431 copper ion Inorganic materials 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000007863 gel particle Substances 0.000 description 1
- 210000003000 inclusion body Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229910052752 metalloid Inorganic materials 0.000 description 1
- 125000001434 methanylylidene group Chemical group [H]C#[*] 0.000 description 1
- 229910001453 nickel ion Inorganic materials 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000037452 priming Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
- ILJSQTXMGCGYMG-UHFFFAOYSA-N triacetic acid Chemical compound CC(=O)CC(=O)CC(O)=O ILJSQTXMGCGYMG-UHFFFAOYSA-N 0.000 description 1
- 229960004799 tryptophan Drugs 0.000 description 1
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Abstract
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Application Number | Priority Date | Filing Date | Title |
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CNB031155162A CN100389325C (zh) | 2003-02-25 | 2003-02-25 | 一种金属螯合层析填料的制备方法 |
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CNB031155162A CN100389325C (zh) | 2003-02-25 | 2003-02-25 | 一种金属螯合层析填料的制备方法 |
Publications (2)
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CN1525165A true CN1525165A (zh) | 2004-09-01 |
CN100389325C CN100389325C (zh) | 2008-05-21 |
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104109190A (zh) * | 2014-07-09 | 2014-10-22 | 广州大学 | 一种制备单一多肽-Zn(Ⅱ)配合物的方法 |
CN106076294A (zh) * | 2016-07-22 | 2016-11-09 | 杨龙 | 一种用于蛋白亲和纯化的金属螯合树脂及其制备方法 |
CN106480011A (zh) * | 2016-12-08 | 2017-03-08 | 南京工业大学 | 一种纯化耦合固定化腺苷酸环化酶的制备方法 |
CN106749499A (zh) * | 2016-11-29 | 2017-05-31 | 生工生物工程(上海)股份有限公司 | 一种高载量Ni‑IDA琼脂糖凝胶的制备方法 |
CN109675535A (zh) * | 2018-12-18 | 2019-04-26 | 杨小丽 | 固定化锌离子亲和层析材料及其制备方法和应用 |
CN112156499A (zh) * | 2020-09-22 | 2021-01-01 | 上海碧云天生物技术有限公司 | 一种金属螯合层析填料及其制备方法 |
CN113231049A (zh) * | 2021-05-11 | 2021-08-10 | 南京工业大学 | 一种交联琼脂糖亲和介质及其制备方法与应用 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1163902A (zh) * | 1996-04-30 | 1997-11-05 | 中国科学院大连化学物理研究所 | 一种分离氨基酸和多肽的介质及其分离方法 |
CN1100568C (zh) * | 1999-04-26 | 2003-02-05 | 中国科学院大连化学物理研究所 | 用于从血浆中去除致病抗体及其复合物的蛋白免疫吸附介质的合成方法 |
CN1160367C (zh) * | 2000-03-16 | 2004-08-04 | 南京师范大学 | 固定化金属离子亲和层析胶的制备技术 |
WO2002037100A2 (en) * | 2000-10-30 | 2002-05-10 | Lytton Simon D | Novel applications of nickel nitrilotriacetic acid (ni-nta) resin: hemeprotein removal, recovery, and purification from biological samples |
-
2003
- 2003-02-25 CN CNB031155162A patent/CN100389325C/zh not_active Expired - Lifetime
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104109190A (zh) * | 2014-07-09 | 2014-10-22 | 广州大学 | 一种制备单一多肽-Zn(Ⅱ)配合物的方法 |
CN104109190B (zh) * | 2014-07-09 | 2018-11-09 | 广州大学 | 一种制备单一多肽-Zn(Ⅱ)配合物的方法 |
CN106076294A (zh) * | 2016-07-22 | 2016-11-09 | 杨龙 | 一种用于蛋白亲和纯化的金属螯合树脂及其制备方法 |
CN106749499A (zh) * | 2016-11-29 | 2017-05-31 | 生工生物工程(上海)股份有限公司 | 一种高载量Ni‑IDA琼脂糖凝胶的制备方法 |
CN106480011A (zh) * | 2016-12-08 | 2017-03-08 | 南京工业大学 | 一种纯化耦合固定化腺苷酸环化酶的制备方法 |
CN109675535A (zh) * | 2018-12-18 | 2019-04-26 | 杨小丽 | 固定化锌离子亲和层析材料及其制备方法和应用 |
CN109675535B (zh) * | 2018-12-18 | 2021-10-22 | 杨小丽 | 固定化锌离子亲和层析材料及其制备方法和应用 |
CN112156499A (zh) * | 2020-09-22 | 2021-01-01 | 上海碧云天生物技术有限公司 | 一种金属螯合层析填料及其制备方法 |
CN113231049A (zh) * | 2021-05-11 | 2021-08-10 | 南京工业大学 | 一种交联琼脂糖亲和介质及其制备方法与应用 |
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CN100389325C (zh) | 2008-05-21 |
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Owner name: SHANGHAI KEHUA BIO-TECHNOLOGY CO., LTD. Free format text: FORMER NAME: SHANGHAI SHIYE KEHUA BIOTECHNOLOGY CO., LTD. |
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Address after: No. 1189 North Qinzhou Road, Shanghai Patentee after: Kehua Biological Technology Co.,Ltd. Shanghai Address before: No. 1189 North Qinzhou Road, Shanghai Patentee before: SHANGHAI KEHUA BIOENGINEERING Co.,Ltd. |
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Owner name: SHANGHAI KEHUA BIO-ENGINEERING CO., LTD. Free format text: FORMER OWNER: SHANGHAI KEHUA BIO-TECHNOLOGY CO., LTD. Effective date: 20090911 |
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C41 | Transfer of patent application or patent right or utility model | ||
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Owner name: SHANGHAI KEHUA BIO-TECHNOLOGY CO., LTD. Free format text: FORMER NAME: SHANGHAI SHIYE KEHUA BIOTECHNOLOGY CO., LTD. |
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CP01 | Change in the name or title of a patent holder |
Address after: No. 1189 North Qinzhou Road, Shanghai Patentee after: Kehua Biological Technology Co.,Ltd. Shanghai Address before: No. 1189 North Qinzhou Road, Shanghai Patentee before: SHANGHAI KEHUA BIOENGINEERING Co.,Ltd. |
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Effective date of registration: 20090911 Address after: No. 1189 North Qinzhou Road, Shanghai Patentee after: SHANGHAI KEHUA BIO-ENGINEERING Co.,Ltd. Address before: No. 1189 North Qinzhou Road, Shanghai Patentee before: Kehua Biological Technology Co.,Ltd. Shanghai |
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Granted publication date: 20080521 |
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