CN1524451A - Degreasing process for cultivated big yellow-fin tunas - Google Patents
Degreasing process for cultivated big yellow-fin tunas Download PDFInfo
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- CN1524451A CN1524451A CNA031155995A CN03115599A CN1524451A CN 1524451 A CN1524451 A CN 1524451A CN A031155995 A CNA031155995 A CN A031155995A CN 03115599 A CN03115599 A CN 03115599A CN 1524451 A CN1524451 A CN 1524451A
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- large yellow
- yellow croaker
- degreasing
- lipase
- cultured large
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Abstract
A cultivated large yellow crocker degreasing process for removing the fat from the cultivated large yellow crockers comprising the steps of, scaling off, cutting, evisceration, cleaning, degreasing through microorganism enzyme in a degreasing pool, seed tank culturing, fermentation tank culturing and filtering, ammonium sulfate deposition, freeze drying, mixing by a finite proportion to obtain microorganism hybrid lipase FE50, the degreased large yellow crocker is dried in low temperature and vacuum-packed for the end product.
Description
Technical field
The present invention relates to the fish food processing technology field, relate in particular to microbial lipase selected when sloughing the marine fish body lipid.
Background technology
Cultured large yellow croaker is because the freezing small fish of long-term use is made feed, cause the desalination of large yellow croaker body colour, sex premature, fatty liver, meat taste poor, especially fat content is too high, reach 35-36%, essential amino acid, be the flavor amino acid content then be starkly lower than wild large yellow croaker, for improving the quality of cultured large yellow croaker, it is carried out ungrease treatment; According to reported in literature, once there were pair fish to carry out the introduction of degreasing in the past, as China's patent of invention " remove the fish fish-skin, fat method " (publication number: 1052992), be the organic acid dipping that adopts higher concentration, the way that stirs, the method will make fish changed beyond recognition, obviously be not suitable for the processing to large yellow croaker; Another patent of invention is " raw meat dried fish processing technology and goods are separated in degreasing " (publication number: 1173298), be to after the upper strata fish peelings such as mackerel, adopt diluted alkaline and degreasing agent LM952, divide secondary to carry out degreasing, each time 15 minutes to half an hour, high-temperature baking is so handled again, for will keeping its original color and luster of cultured large yellow croaker, and the product that presents the taste of wild large yellow croaker can't be realized.
Summary of the invention
The objective of the invention is to slough unnecessary subcutaneous fat and the moisture content in the muscle, keep original color and luster of large yellow croaker, make it have the delicious taste of wild large yellow croaker and a kind of cultured large yellow croaker degreasing processing technology of providing in order to improve the quality of cultured large yellow croaker.
For achieving the above object, the present invention has adopted following technical scheme: cultured large yellow croaker is scaled, hack, take out internal organ, through cleaning, put into the degreasing pond and carry out degreasing with microbial enzyme, degreasing is after the low temperature appropriateness is dry, and vacuum-packed back is a finished product;
Said microbial enzyme is the higher mixing-in fat enzyme FE50 of a kind of vigor, system has the lipase that stronger narrow spectrum microbial strains produces to the cultured large yellow croaker fat splitting, it is to select for use to disclose commercially available fold candida (Candida rugosa), penicillium expansum (Penicillium expansum), aspergillus niger (Aspergillus niger), penicillium cyclopium four kinds of microbial strains such as (Penicillium cyclopium) are through instructionization, the ultraviolet ray variation, strain separating, slant pore is cultivated, seed tank culture, fermentation tank culture and filtration, ammonium sulfate precipitation, obtain four kinds of microbial lipases after the freeze drying, through certain proportion mix microorganism mixing-in fat enzyme FE50;
Said instructionization is to place the culture medium that contains sea-farming large yellow croaker fish oil to cultivate resulting microorganism fungus kind behind the certain hour respectively four kinds of microorganisms such as fold candida, penicillium expansum, aspergillus niger, penicillium cyclopium;
Said ultraviolet ray variation is that four kinds of microorganisms such as fold candida, penicillium expansum, aspergillus niger, penicillium cyclopium are placed respectively under certain intensity uviol lamp, behind the irradiation certain hour, the cultured large yellow croaker grease there is the process of the microorganism fungus kind of hydrolysis ability by force through what the screening separation obtained.
Said seed tank culture, fermentation tank culture, its culture medium composition (g/l): glucose 10, cultured large yellow croaker fish oil 30, urea 2, KH
2PO
46, K
2HPO
42.1, MgSO
40.1, inositol 4 * 10
-6, need having of independent filtration sterilization in addition: biotin 8 * 10
-6, Cobastab
12 * 10
-4, its condition of culture: loading capacity is 70%, speed of agitator 450 commentaries on classics/min, throughput 2m
3/ min.m
3, cultivation temperature is 30 ℃, incubation time is 58h;
Said ammonium sulfate precipitation, candida rugosa lipase is got the precipitation part of ammonium sulfate concentrations between 38-42%, penicillium expansum lipase is got the precipitation part of ammonium sulfate concentrations between 47-50%, aspergillus niger lipase is got the precipitation part of ammonium sulfate concentrations between 33-38%, and penicillium cyclopium lipase is got the precipitation part of ammonium sulfate concentrations between 44-49%.
Owing to adopted technique scheme, when the mixing-in fat enzyme FE50 that makes with technology of the present invention is used for sea-farming large yellow croaker fat and sloughs, remaining lipid of fish body and wild large yellow croaker basically identical; And FE50 is selective to fat splitting for the mixing-in fat enzyme, from 8 carbon to 18 carbon fatty acid glyceride, effectively hydrolysis of mixing-in fat enzyme FE50, but poor to the fatty glyceride hydrolysis ability of 20-22 carbon, thus product still is rich in EPA, DHA after guaranteeing degreasing; Degreasing large yellow croaker fish surface after processing is handled is the original color and luster of large yellow croaker, and the surface is glossy, and shape is complete, and muscle is white in color or is light yellow, and is closely solid flexible; Peculiar fragrance with drying fish dried fish, taste and wild large yellow croaker are as good as; And dry, vacuum-packed through the low temperature appropriateness, for storing has brought convenience.
The specific embodiment
The present invention is further illustrated with most preferred embodiment below.
Cultured large yellow croaker degreasing processing technology embodiment of the present invention:
1, selects fresh and alive cultured large yellow croaker about 0.5kg for use.
2, scale, the back hacks internal organ.
3, clean.
4, degreasing: the fish that will clean is put into the degreasing pond of mixing-in fat enzyme FE50, the weight ratio of fish and water is 1: 1, and the concentration of each lipase and proportioning are candida rugosa lipase among the mixing-in fat enzyme FE50: penicillium expansum lipase: aspergillus niger lipase: penicillium cyclopium lipase: 10unit/ml: 10unit/ml: 10unit/ml: 10unit/ml; PH is controlled at 9.0-9.4; Degreasing time 45-60min, it is 0.02-0.03m that air-flow stirs throughput
3/ min.m
2Surface area.
Wherein mixing-in fat enzyme FE50 production technology is:
1. select fold candida (Candida rugosa), penicillium expansum (Penicilliumexpansum), aspergillus niger (Aspergillus niger), four kinds of microbial strains of penicillium cyclopium (Penicilliumcyclopium) for use.
2. place the culture medium that contains 3% cultured large yellow croaker fish oil to cultivate its culture medium composition (g/l): glucose 10, cultured large yellow croaker fish oil 30, urea 2, KH one month respectively
2PO
46, K
2HPO
42.1, MgSO
40.1, inositol 4 * 10
-6
3. be 15KW/m at uitraviolet intensity
2Shine 60min under the condition.
4. place the culture medium that contains 3% cultured large yellow croaker fish oil to cultivate its culture medium composition (g/l): glucose 10, cultured large yellow croaker fish oil 30, urea 2, KH 3 days again
2PO
46, K
2HPO
42.1, MgSO
40.1, inositol 4 * 10
-6, select respectively to the stronger colony inoculation of cultured large yellow croaker fish oil hydrolysis ability cultivate fold candida (Candida rugosa), penicillium expansum (Penicillium expansum), aspergillus niger (Aspergillus niger), four kinds of microbial strains of penicillium cyclopium (Penicillium cyclopium).
5. seed tank culture, fermentation tank culture, its culture medium composition (g/l): glucose 10, cultured large yellow croaker fish oil 30, urea 2, KH
2PO
46, K
2HPO
42.1, MgSO
40.1, inositol 4 * 10
-6, need having of independent filtration sterilization in addition: biotin 8 * 10
-6, Cobastab
12 * 10
-4, its condition of culture: loading capacity is 70%, speed of agitator 450 commentaries on classics/min, throughput 2m
3/ min.m
3, cultivation temperature is 30 ℃, incubation time is 58h.
6. four layers of filtered through gauze get filtrate.
7. ammonium sulfate precipitation, candida rugosa lipase is got the precipitation part of ammonium sulfate concentrations between 38-42%, penicillium expansum lipase is got the precipitation part of ammonium sulfate concentrations between 47-50%, aspergillus niger lipase is got the precipitation part of ammonium sulfate concentrations between 33-38%, and penicillium cyclopium lipase is got the precipitation part of ammonium sulfate concentrations between 44-49%.
8. at-5～-10 ℃, vacuum is freeze drying 10 hours under the 759.9mmHg condition, and is stand-by.
Mix by a certain percentage when 9. using.
5, low temperature drying will place 15-20 ℃ of following low temperature drying 36 hours through the cultured large yellow croaker after the above-mentioned processing.
6, vacuum packaging.
Claims (7)
1, a kind of cultured large yellow croaker degreasing processing technology is characterized in that: cultured large yellow croaker is scaled, hack, take out internal organ, through cleaning, put into the degreasing pond and carry out degreasing with microbial enzyme, degreasing is after the low temperature appropriateness is dry, and vacuum-packed back is a finished product.
2, a kind of cultured large yellow croaker degreasing processing technology according to claim 1, it is characterized in that: said microbial enzyme is to adopt the higher mixing-in fat enzyme FE50 of a kind of vigor, system has the lipase that stronger narrow spectrum microbial strains produces to the cultured large yellow croaker fat splitting, it is to select for use to disclose commercially available fold candida (Candida rugosa), penicillium expansum (Penicillium expansum), aspergillus niger (Aspergillus niger), penicillium cyclopium four kinds of microbial strains such as (Penicillium cyclopium) are through instructionization, the ultraviolet ray variation, strain separating, slant pore is cultivated, seed tank culture, fermentation tank culture and filtration, ammonium sulfate precipitation, obtain four kinds of microbial lipases after the freeze drying, through certain proportion mix microorganism mixing-in fat enzyme FE50.
3, a kind of cultured large yellow croaker degreasing processing technology according to claim 2, it is characterized in that: said instructionization is to place the culture medium that contains 3% cultured large yellow croaker fish oil to cultivate respectively four kinds of microorganisms such as fold candida, penicillium expansum, aspergillus niger, penicillium cyclopium, and incubation time is one month; Its culture medium composition (g/l): glucose 10, cultured large yellow croaker fish oil 30, urea 2, KH
2PO
46, K
2HPO
42.1, MgSO
40.1, inositol 4 * 10
-6
4, a kind of cultured large yellow croaker degreasing processing technology according to claim 2, it is characterized in that: the variation of said ultraviolet is to be 15KW/m with fold candida (Candida rugosa), penicillium expansum (Penicillium expansum), aspergillus niger (Aspergillus niger), penicillium cyclopium four kinds of microorganisms such as (Penicillium cyclopium) at uitraviolet intensity
2Under shine 60min after, through screening separate obtain the cultured large yellow croaker grease is had the process of the microorganism fungus kind of strong hydrolysis ability.
5, a kind of cultured large yellow croaker degreasing processing technology according to claim 2 is characterized in that: said seed tank culture, fermentation tank culture, its culture medium composition (g/l): glucose 10, cultured large yellow croaker fish oil 30, urea 2, KH
2PO
46, K
2HPO
42.1, MgSO
40.1, inositol 4 * 10
-6, need having of independent filtration sterilization in addition: biotin 8 * 10
-6, Cobastab
12 * 10
-4, its condition of culture: loading capacity is 70%, speed of agitator 450 commentaries on classics/min, throughput 2m
3/ min.m
3, cultivation temperature is 30 ℃, incubation time is 58h.
6, a kind of cultured large yellow croaker degreasing processing technology according to claim 2, it is characterized in that: said ammonium sulfate precipitation, candida rugosa lipase is got the precipitation part of ammonium sulfate concentrations between 38-42%, penicillium expansum lipase is got the precipitation part of ammonium sulfate concentrations between 47-50%, aspergillus niger lipase is got the precipitation part of ammonium sulfate concentrations between 33-38%, and penicillium cyclopium lipase is got the precipitation part of ammonium sulfate concentrations between 44-49%.
7, a kind of cultured large yellow croaker degreasing processing technology according to claim 1, it is characterized in that: said degreasing, it is the degreasing pond of the fish of cleaning being put into mixing-in fat enzyme FE50, the weight ratio of fish and water is 1: 1, and the concentration of each lipase and proportioning are candida rugosa lipase among the mixing-in fat enzyme FE50: penicillium expansum lipase: aspergillus niger lipase: penicillium cyclopium lipase: 10unit/ml: 10unit/ml: 10unit/ml: 10unit/ml; PH is controlled at 9.0-9.4; Degreasing time 45-60min, it is 0.02-0.03m that air-flow stirs throughput
3/ min.m
2Surface area.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA031155995A CN1524451A (en) | 2003-02-26 | 2003-02-26 | Degreasing process for cultivated big yellow-fin tunas |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA031155995A CN1524451A (en) | 2003-02-26 | 2003-02-26 | Degreasing process for cultivated big yellow-fin tunas |
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| Publication Number | Publication Date |
|---|---|
| CN1524451A true CN1524451A (en) | 2004-09-01 |
Family
ID=34284356
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103932243A (en) * | 2014-04-30 | 2014-07-23 | 江南大学 | Method for improving flavor of liquor-marinated fish by utilizing biological flavor increasing technique |
| CN104544312A (en) * | 2014-12-24 | 2015-04-29 | 广东省农业科学院蚕业与农产品加工研究所 | Semi-fermented low-salt fish leisure food and production method thereof |
| CN104856083A (en) * | 2015-04-24 | 2015-08-26 | 安徽卫食园肉类食品有限公司 | Production technology of dried ducks with cooking liquor |
| CN111631361A (en) * | 2020-05-15 | 2020-09-08 | 中国水产科学研究院南海水产研究所 | Method for reducing fish lipid of trachinotus ovatus by using complex enzyme |
-
2003
- 2003-02-26 CN CNA031155995A patent/CN1524451A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103932243A (en) * | 2014-04-30 | 2014-07-23 | 江南大学 | Method for improving flavor of liquor-marinated fish by utilizing biological flavor increasing technique |
| CN103932243B (en) * | 2014-04-30 | 2016-06-15 | 江南大学 | A kind of method utilizing the biological fragrant liquor-saturated fish local flavor of skill upgrading |
| CN104544312A (en) * | 2014-12-24 | 2015-04-29 | 广东省农业科学院蚕业与农产品加工研究所 | Semi-fermented low-salt fish leisure food and production method thereof |
| CN104544312B (en) * | 2014-12-24 | 2017-06-30 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of half-fermented less salt flesh of fish leisure food and preparation method thereof |
| CN104856083A (en) * | 2015-04-24 | 2015-08-26 | 安徽卫食园肉类食品有限公司 | Production technology of dried ducks with cooking liquor |
| CN111631361A (en) * | 2020-05-15 | 2020-09-08 | 中国水产科学研究院南海水产研究所 | Method for reducing fish lipid of trachinotus ovatus by using complex enzyme |
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