CN1468625A - Neem oil prepn for preventing and treating parasitic disease of aquatic animals and its prepn process - Google Patents
Neem oil prepn for preventing and treating parasitic disease of aquatic animals and its prepn process Download PDFInfo
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- CN1468625A CN1468625A CNA031177271A CN03117727A CN1468625A CN 1468625 A CN1468625 A CN 1468625A CN A031177271 A CNA031177271 A CN A031177271A CN 03117727 A CN03117727 A CN 03117727A CN 1468625 A CN1468625 A CN 1468625A
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Abstract
The neem oil preparation consists of neem oil, water, Tween-60 or Tween-80, Span-60 or Span-80, citric acid, potassium sorbate, EDTA, and nimbin or natural avermectin. The neem oil preparation has high effect of preventing and treating parasitic disease of aquatic animals and no resistance, owing to the functional mechanism including lowering intestinal tract activity, inhibiting ecdysis, inhibiting mating and oviposition, refusing feed, inhibiting egg development, etc. In addition, the present invention has low cost.
Description
Technical field
The present invention relates to neem oil preparation control parasite in aquiculture preparation and preparation method thereof.
Background technology
China is the country of aquaculture maximum, annual because the parasite in culturing is as Trichodina, little melonworm, ciliate, sporozoon, oblique tube insect, harm such as dactylogyrider have caused tremendous loss for the Aquatic product aquaculture, in the process of control parasite in aquiculture, now a large amount of uses is as metrifonate, potassium permanganate, a series of chemicals such as malachite green oxalate, although this a series of chemicals plays an important role in the process of control parasite in aquiculture, but its natural degradation in water body is slow, target worm drug resistance constantly strengthens, cause the increasing vicious cycle of dosage, the use chemicals also makes the fish in the aquaculture, shrimp, intravital drug residue such as Eriocheir sinensis exceeds standard, and has influenced the outlet of China's breed series products.
But existing nimbin or neem oil preparation are the plant class parasite killing medications of universally acknowledged safe natural degradation, and existing nimbin or neem oil preparation substantially all are to be used for this purpose.Do not see the control that is useful on parasite in aquiculture.And there is the preparation lamination in existing nimbin emulsion preparation, could use after need shaking up before using.The solvent that the nimbin emulsion preparation uses, emulsifying agent contain benzene class, methanol class mostly as the material of developed country's forbidding, and be bigger to environmental hazard, offset improvement effect and the ecological protection effect of nimbin to environment.
Summary of the invention
The purpose of this invention is to provide a kind of parasite in aquiculture is prevented and treated and compose extensively, consumption is little, and raw material and goods do not have substantially to vertebrates causes harm, environmentally safe neem oil preparation control parasite in aquiculture preparation and preparation method thereof.
Another object of the present invention provides the new purposes of nimbin or neem oil preparation, promptly is used for the control of parasite in aquiculture.
The present invention is the biological product based on the Azadirachta Indica A. vegetable oil, and its principle is for to be made into the aqueous emulsion that contains neem oil to the Azadirachta Indica A. vegetable oil by emulsification, the target worm is killed after during use aqueous emulsion being diluted to certain concentration.Preparation of the present invention is made up of following:
Contain neem oil 1～49%, water 50～98%, polysorbate60 or Tween 80 0.1～5%, span (Speen) 60 or span (Speen) 80 0.05～5%.
Above-mentioned preparation also contains the citric acid 0.01～5% that accounts for above-mentioned total amount, potassium sorbate 0.01～5%, EDTA0.01～5%, nimbin 0.001～5% or natural pyrethrum 0.001～5%.
The preparation method of above-mentioned neem oil preparation control parasite in aquiculture preparation is carried out according to the following steps:
Heat water to 50～60 ℃, add soil temperature 60, sorbester p18 or add soil temperature 80 and sorbester p17, the material that is stirred to adding dissolves fully, and under stirring, add neem oil, continue agitating solution to turning white, input high pressure homogenizer homogenizing 4 times under the pressure of 300～600Mpa is finished the preparation of neem oil emulsified water Emulsion.
Add citric acid, potassium sorbate, EDTA, nimbin or natural pyrethrum behind the homogenizing finishing for the first time.
Preparation of the present invention has the better prevention effect for parasite in aquiculture.Neem oil emulsified water Emulsion does not have lamination, need not shake up before the use.Because preparation of the present invention acts on the mechanism of action of the parasitic target worm of culturing object and is respectively: reduce the intestinal vigor; Suppress decortication; Suppress copulation, lay eggs; Refusing to eat; Suppress effects such as egg development, so these goods of life-time service target insect species can not develop immunity to drugs yet.If is 13～15 yuan with existing nimbin preparation as every mu of use cost of a kind of biological insecticides, only be 2～4 yuan and use preparation of the present invention to reach same every mu of use cost of insecticidal effect, therefore help promoting the use of of these biological insecticides.
It below is the experiment effect that preparation of the present invention is used to prevent and treat parasite in aquiculture.
1, Trichodina is killed experiment:
Experiment fingerling: Ctenopharyngodon idellus fry;
Experimental facilities: small-sized fishpond;
Experimental technique: contrast experiment;
Experimental drug: preparation of the present invention;
Experiment Ctenopharyngodon idellus symptom: there are a large amount of mucous secretions in the tissue inflammation of the fish body skin and the gill; The fish body turns black, becomes thin, and moves about slowly; Microscopy finds that the body surface and the gill of fish have the Trichodina parasitism.
According to experiment pool area full pool spilling head preparation 0.5PPM of the present invention, contrast groups is not offerd medicine at experimental group.
Situation after the medication:
First day microscopy: the experimental group worm is not dead; The contrast groups worm is not dead.
Second day microscopy: experimental group part worm is not dead; The contrast groups worm is not dead.
The 3rd day microscopy: the experimental group worm is dead entirely; The contrast groups worm is not dead.
The 4th day microscopy: the experimental group worm is dead entirely; The contrast groups worm is not dead.
The 5th day microscopy: the experimental group worm is dead entirely; The contrast groups worm is not dead.
Experiment shows that the Azadirachta Indica A. preparation has good killing action to Trichodina.
2, oblique tube insect is killed experiment:
Experiment fingerling: Ctenopharyngodon idellus fry;
Experimental facilities: small-sized fishpond;
Experimental technique: contrast experiment;
Experimental drug: preparation of the present invention;
Experiment Ctenopharyngodon idellus symptom: the fish body skin and the gill have a large amount of mucous secretions and have the blue membranoid substance gill to be white; The fish dyspnea; Microscopy finds that the body surface and the gill of fish have a large amount of oblique tube insect parasitisms.
According to experiment pool area full pool spilling head preparation 0.5PPM of the present invention, contrast groups is not offerd medicine at experimental group.
Situation after the medication:
First day microscopy: the experimental group worm is not dead; The contrast groups worm is not dead.
Second day microscopy: experimental group part worm is not dead; The contrast groups worm is not dead.
The 3rd day microscopy: the experimental group worm is dead entirely; The contrast groups worm is not dead, and the death of part fish is arranged.
The 4th day microscopy: the experimental group worm is dead entirely; The contrast groups worm is not dead, and the death of part fish is arranged.
The 5th day microscopy: the experimental group worm is dead entirely; The contrast groups worm is not dead, and the death of part fish is arranged.
Experiment shows that the Azadirachta Indica A. preparation has good killing action to oblique tube insect.
3, the latent whipworm of the gill is killed experiment:
Experiment fingerling: Ctenopharyngodon idellus;
Experimental facilities: small-sized fishpond;
Experimental technique: contrast experiment;
Experimental drug: Azadirachta Indica A. preparation;
Experiment Ctenopharyngodon idellus symptom: the fish gill is rubescent, and mucus is arranged, dyspnea, and fish does not ingest, and microscopy finds that the fish gill has the gill to conceal the whipworm parasitism.
According to experiment pool area full pool spilling head preparation 0.5PPM of the present invention, contrast groups is not offerd medicine at experimental group.
Situation after the medication:
First day microscopy: the experimental group worm is not dead; The contrast groups worm is not dead.
Second day microscopy: experimental group part worm is not dead; The contrast groups worm is not dead.
The 3rd day microscopy: the experimental group worm is dead entirely; The contrast groups worm is not dead.
The 4th day microscopy: the experimental group worm is dead entirely; The contrast groups worm is not dead.
The 5th day microscopy: the experimental group worm is dead entirely; The contrast groups worm is not dead.
Experiment shows that the latent whipworm of Azadirachta Indica A. preparation zygobranchiate has good killing action.
4, ciliate is killed experiment:
Experiment fingerling: Eriocheir sinensis:
Experimental facilities: small-sized Eriocheir sinensis pond;
Experimental technique: contrast experiment;
Experimental drug: preparation of the present invention;
Experiment Eriocheir sinensis symptom: the visible a large amount of floss hairs of Eriocheir sinensis body surface, hands has been touched soapy feeling; Microscopy finds that the position parasitisms such as the gill, body surface and appendage of crab have a large amount of ciliate.
According to experiment pool area full pool spilling head preparation 0.4PPN of the present invention, contrast groups is not offerd medicine at experimental group.
Situation after the medication:
First day microscopy: the experimental group worm is not dead; The contrast groups worm is not dead.
Second day microscopy: experimental group part worm is not dead; The contrast groups worm is not dead.
The 3rd day microscopy: the experimental group worm is dead entirely; The contrast groups worm is not dead.
The 4th day microscopy: the experimental group worm is dead entirely; The contrast groups worm is not dead.
The 5th day microscopy: the experimental group worm is dead entirely; The contrast groups worm is not dead.
Experiment shows that the Azadirachta Indica A. preparation has good killing action to ciliate.
5, dactylogyrider is killed experiment:
Experiment fingerling: Anguillar japonica;
Experimental facilities: small-sized eel culture pond;
Experimental technique: contrast experiment;
Experimental drug: preparation of the present invention:
Experiment Anguillar japonica symptom: the secretion of Anguillar japonica gill mucus increases, and the gill filament is seriously congested; Microscopy finds that a large amount of ocellate Folium Salicis Babylonicae shape polypides show that a large amount of dactylogyriders are arranged.
According to experiment pool area full pool spilling head preparation 1PPM of the present invention, contrast groups is not offerd medicine at experimental group.
Situation after the medication:
First day microscopy: the experimental group worm is not dead; The contrast groups worm is not dead.
Second day microscopy: experimental group part worm is not dead; The contrast groups worm is not dead.
The 3rd day microscopy: the experimental group worm is dead entirely; The contrast groups worm is not dead.
The 4th day microscopy: the experimental group worm is dead entirely; The contrast groups worm is not dead.
The 5th day microscopy: the experimental group worm is dead entirely; The contrast groups worm is not dead.
Experiment shows that the Azadirachta Indica A. preparation has good killing action to dactylogyrider.
6, Gyrodactylus is killed experiment:
Experiment fingerling: Anguillar japonica;
Experimental facilities: small-sized eel culture pond;
Experimental technique: contrast experiment;
Experimental drug: preparation of the present invention;
Experiment Anguillar japonica symptom: the secretion of Anguillar japonica gill mucus increases, and the gill filament is seriously congested; Microscopy finds that the Folium Salicis Babylonicae shape of big measurer and body tubbiness polypide show that a large amount of Gyrodactylus are arranged.
According to experiment pool area full pool spilling head preparation 1PPM of the present invention, contrast groups is not offerd medicine at experimental group.
Situation after the medication:
First day microscopy: the experimental group worm is not dead; The contrast groups worm is not dead.
Second day microscopy: experimental group part worm is not dead; The contrast groups worm is not dead.
The 3rd day microscopy: the experimental group worm is dead entirely; The contrast groups worm is not dead.
The 4th day microscopy: the experimental group worm is dead entirely; The contrast groups worm is not dead.
The 5th day microscopy: the experimental group worm is dead entirely; The contrast groups worm is not dead.
Experiment shows that the Azadirachta Indica A. preparation has good killing action to Gyrodactylus.
7, little melonworm is killed experiment:
Experiment fingerling: Cyprinus carpio L. fish;
Experimental facilities: small-sized crawl;
Experimental technique: contrast experiment;
Experimental drug: preparation of the present invention;
Experiment Cyprinus carpio L. symptom: microscopy is sent out the Cyprinus carpio L. parasitism a large amount of little melonworms.
According to experiment pool area full pool spilling head preparation 1PPM of the present invention, contrast groups is not offerd medicine at experimental group.
Situation after the medication:
First day microscopy: the experimental group worm is not dead; The contrast groups worm is not dead.
Second day microscopy: experimental group part worm is not dead; The contrast groups worm is not dead.
The 3rd day microscopy: the experimental group worm is dead entirely; The contrast groups worm is not dead.
The 4th day microscopy: the experimental group worm is dead entirely; The contrast groups worm is not dead.
The 5th day microscopy: the experimental group worm is dead entirely; The contrast groups worm is not dead.
Experiment shows that the Azadirachta Indica A. preparation has the good killing action of root to little melonworm.
The specific embodiment
Embodiment 1:
Get neem oil 19%, water 80%, polysorbate60 0.7%, sorbester p18 0.3%; Heat water to 50～60 ℃ 2, add soil temperature 60, sorbester p18, the material that is stirred to adding dissolves fully, and adds neem oil under stirring, continues agitating solution to turning white, the input high pressure homogenizer is behind homogenizing under the pressure of 300Mpa 1 time, the citric acid 0.1% that adds above-mentioned total amount, potassium sorbate 0.09%, EDTA0.1%, carry out homogenizing again 3 times behind nimbin 0.005% or natural pyrethrum 0.008% mixing, finish the preparation of neem oil emulsified water Emulsion.
Embodiment 2:
Get neem oil 1%, water 94%, polysorbate60 3%, sorbester p18 2%; Heat water to 50～60 ℃, add soil temperature 60, sorbester p18, the material that is stirred to adding dissolves fully, and adds neem oil under stirring, continues agitating solution to turning white, the input high pressure homogenizer is behind homogenizing under the pressure of 600Mpa 1 time, the citric acid 0.01% that adds above-mentioned total amount, potassium sorbate 0.01%, EDTA0.01%, carry out homogenizing again 3 times behind nimbin 0.001% or natural pyrethrum 0.005% mixing, finish the preparation of neem oil emulsified water Emulsion.
Embodiment 3:
Get neem oil 49%, water 50%, Tween 80 0.65%, sorbester p17 0.35% is undertaken by the step of embodiment 1, adds the citric acid 0.18% of above-mentioned total amount, potassium sorbate 0.08%, EDTA0.1%, nimbin 0.05% or natural pyrethrum 0.01%; Following steps are still with embodiment 1.
Claims (5)
1, a kind of with neem oil preparation control parasite in aquiculture preparation, it is characterized in that containing neem oil 1～49%, water 50～98%, polysorbate60 or Tween 80 0.1～5%, sorbester p18 or sorbester p17 0.05～5%.
2, according to claim 1 with neem oil preparation control parasite in aquiculture preparation, it is characterized in that also containing the citric acid 0.01～5% that accounts for above-mentioned total amount, potassium sorbate 0.01～5%, EDTA0.01～5%, nimbin 0.001～5% or natural pyrethrum 0.001～5%.
3, according to claim 1,2 described preparation methoies with neem oil preparation control parasite in aquiculture preparation, it is characterized in that carrying out according to the following steps: heat water to 50～60 ℃, add soil temperature 60, sorbester p18 or add soil temperature 80 and sorbester p17, the material that is stirred to adding dissolves fully, and under stirring, add neem oil, continue agitating solution to turning white, input high pressure homogenizer homogenizing 4 times under the pressure of 300～600Mpa is finished the preparation of neem oil emulsified water Emulsion.
4, the preparation method with neem oil preparation control parasite in aquiculture preparation according to claim 3 is characterized in that adding citric acid, potassium sorbate, EDTA, nimbin or natural pyrethrum behind the homogenizing finishing for the first time.
5, the purposes with neem oil preparation control parasite in aquiculture preparation according to claim 1 is characterized in that being used for the control of parasite in aquiculture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03117727 CN1259930C (en) | 2003-04-18 | 2003-04-18 | Neem oil prepn for preventing and treating parasitic disease of aquatic animals and its prepn process |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03117727 CN1259930C (en) | 2003-04-18 | 2003-04-18 | Neem oil prepn for preventing and treating parasitic disease of aquatic animals and its prepn process |
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| Publication Number | Publication Date |
|---|---|
| CN1468625A true CN1468625A (en) | 2004-01-21 |
| CN1259930C CN1259930C (en) | 2006-06-21 |
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| CN 03117727 Expired - Fee Related CN1259930C (en) | 2003-04-18 | 2003-04-18 | Neem oil prepn for preventing and treating parasitic disease of aquatic animals and its prepn process |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006029896A1 (en) * | 2004-09-17 | 2006-03-23 | Aquanova German Solubilisate Technologies (Agt) Gmbh | Preservative compositions |
| CN103081894A (en) * | 2013-01-25 | 2013-05-08 | 江苏省苏科农化有限责任公司 | Novel preparation method of pesticide emulsion in water |
| CN103539537A (en) * | 2013-10-12 | 2014-01-29 | 合肥科友生物科技有限公司 | Organic-inorganic composite biological medicinal fertilizer exclusively used for silver carp and preparation method thereof |
| CN103990117A (en) * | 2014-04-25 | 2014-08-20 | 珠海市康益达生物科技有限公司 | Nuisance-free antibiotic insecticidal preparation for aquatic product culturing, and preparation method thereof |
| CN105918702A (en) * | 2016-04-29 | 2016-09-07 | 成都绿金生物科技有限责任公司 | Neem oil feed for fish and preparation method thereof |
| CN112334007A (en) * | 2018-06-13 | 2021-02-05 | 发酵专家公司 | Antiparasitic compositions |
-
2003
- 2003-04-18 CN CN 03117727 patent/CN1259930C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006029896A1 (en) * | 2004-09-17 | 2006-03-23 | Aquanova German Solubilisate Technologies (Agt) Gmbh | Preservative compositions |
| CN103081894A (en) * | 2013-01-25 | 2013-05-08 | 江苏省苏科农化有限责任公司 | Novel preparation method of pesticide emulsion in water |
| CN103539537A (en) * | 2013-10-12 | 2014-01-29 | 合肥科友生物科技有限公司 | Organic-inorganic composite biological medicinal fertilizer exclusively used for silver carp and preparation method thereof |
| CN103539537B (en) * | 2013-10-12 | 2015-03-11 | 合肥科友生物科技有限公司 | Organic-inorganic composite biological medicinal fertilizer exclusively used for silver carp and preparation method thereof |
| CN103990117A (en) * | 2014-04-25 | 2014-08-20 | 珠海市康益达生物科技有限公司 | Nuisance-free antibiotic insecticidal preparation for aquatic product culturing, and preparation method thereof |
| CN105918702A (en) * | 2016-04-29 | 2016-09-07 | 成都绿金生物科技有限责任公司 | Neem oil feed for fish and preparation method thereof |
| CN112334007A (en) * | 2018-06-13 | 2021-02-05 | 发酵专家公司 | Antiparasitic compositions |
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| Publication number | Publication date |
|---|---|
| CN1259930C (en) | 2006-06-21 |
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Granted publication date: 20060621 |