CN1451323A - Biologic enzyme treatment method for promoting natural alcoholization of re-flue-cured tobacco - Google Patents

Biologic enzyme treatment method for promoting natural alcoholization of re-flue-cured tobacco Download PDF

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CN1451323A
CN1451323A CN 02116594 CN02116594A CN1451323A CN 1451323 A CN1451323 A CN 1451323A CN 02116594 CN02116594 CN 02116594 CN 02116594 A CN02116594 A CN 02116594A CN 1451323 A CN1451323 A CN 1451323A
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China
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gram
tobacco leaf
tobacco
flue
natural alcoholization
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CN1194642C (en
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孙君社
马林
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Jilin Tobacco Industrial Co Ltd
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China Agricultural University
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Abstract

A biologic enzyme process for promoting natural alcoholization of baked tobacco leaves is characterized by that the proteinase and pectinase are used to treat tobacco leaves for degradating the protein and pectin in them and the promoters of biochemical reaction, such as pyruvic acid, tryptophan, leucine and metallic ions [Mn(2+), Zn(2+), Mg(2+) and K(+)] use used to activate the activity of enzyme contained in tobacco leaves. Its advantages are short period shortened from 2-3 years to 6 months and high quality of tobacco.

Description

A kind of biologic enzyme treatment method that promotes natural alcoholization of re-flue-cured tobacco
Technical field
The present invention relates to the application in tobacco natural alcoholization process of biological enzyme technology and biologically active catalytic specie, International Classification of Patents belongs to A24B.
Background technology
Tobacco be a kind of be the special economic crop of purpose to suck, its use value depends on its quality.Perfume is jealous to be the important content of quality of tobacco key element, and sound tobacco requires fragrance matter pure, the perfume quantity abundance, and jealous pure and mild, pleasant impression is comfortable.To roll into cigarette product be a complicated system engineering to tobacco leaf from planting to, experienced the jealous relevant physiology of the many and tobacco incense that comprises tobacco mellowing therebetween, biochemistry, processes such as technology.Beating and double roasting is to solve the basic skills that has defective on the results quality of tobacco, what be about to the purchase of cigarette station first roastingly transports to Redrying Factory to shape cigarette (also claiming former cigarette) and passes through the baking second time again, the full wafer cigarette is made into certain specification fragment cigarette, desinsection in the beating and double roasting process, promote blue or green cigarette to change yellow, abirritate and assorted gas, and the control moisture content in leaves prevents tobacco leaf infested going mouldy in the storage process in 11%~13% scope.The fragment cigarette is deposited in the tobacco mellowing warehouse after packing through machinery, stores under the natural alcoholization condition 2~3 years, tobacco leaf its fragrant jealous quality behind natural alcoholization has clear improvement and improves, but the alcoholization excessive cycle takies substantial contribution and storehouse, and production cost is higher.
Biotechnology especially microbial fermentation and the application of zymotechnic in tobacco gradually becomes hot subject, the main contents of research comprise from tobacco leaf surface separates some probiotics, put on tobacco surface (tobacco science and technology through increasing the bacterium cultivation, 1998 (1)), also have and use cellulase partly the degrade lignin and the cellulose (U.S.pat.3747608) of offal, be convenient to process stem and reduce the assorted gas of stem xylon gas sample to reach softening offal, utilize and produce fragrant microbial fermentation production tobacco aromatics using (tobacco science and technology, 1997, (1)), microorganism puts in the natural alcoholization tobacco, because the natural alcoholization moisture content in leaves is 11-13%, growth of microorganism is suppressed, and action effect is poor, microbial biomass is wayward in addition, may cause cigarette finished product depositing, the variation of its quality during sale.The cellulase high offal of content of cellulose that suits is unsuitable for tobacco leaf.
Summary of the invention
The objective of the invention is to provide the biologic enzyme treatment method of a kind of promotion beating and double roasting tobacco leaf (being also referred to as the beating and double roasting blade) natural alcoholization, this method should be able to shorten the natural alcoholization of re-flue-cured tobacco cycle, improves the fermenting tobacco leaf quality.
Be included in the mixture that adds protease and pectase in the beating and double roasting tobacco leaf in order to reach the technical scheme that purpose of the present invention takes; Technical scheme of the present invention can also comprise adding biochemical reaction promoter in tobacco leaf, as pyruvic acid, tryptophan, leucine and configuration metal ions Zn 2+, Mn 2+, Mg 2+And K +
After the above-mentioned redrying blade packing of having added enzyme and biochemical reaction promoter, be deposited in the tobacco mellowing warehouse, under the natural alcoholization condition, stored 3~12 months.
Above-mentioned technical scheme is characterised in that, the ratio that adds protease and pectase in the beating and double roasting tobacco leaf is 4: 1 a mixture, and this mixture adding proportion is to account for 0.06~0.3% of tobacco leaf weight; Can also adding proportion be the pyruvic acid of tobacco leaf weight 0.01~0.04% in tobacco leaf, adding proportion be tryptophan, the leucine of tobacco leaf weight 0.01~0.03%, and adding proportion is the configuration metal ions Zn of tobacco leaf weight 0.001~0.005% 2+, Mn 2+, Mg 2+, Zn 2+, Mn 2+, Mg 2+Ratio be 1: 1: 1, adding proportion is the K of tobacco leaf weight 0.1~0.5% +
Natural alcoholization optimum value storage period in the technique scheme is about 6 months, is better than the quality of tobacco in 3 years of common alcoholization through this quality of handling tobacco leaf, can satisfy the requirement of cigarette processing.
The beating and double roasting tobacco leaf generally needs to handle through the natural alcoholization in 2~3 years, make the inner chemical composition of tobacco leaf be tending towards harmony, quality just can be transported to cigar mill's processing cigarette after improving, for shortening the alcoholization cycle, improve the alcoholization quality of tobacco, the present invention has adopted protease, pectase biological enzyme formulation, add the pectin that pectase can degrade in the tobacco leaf and generate monose, help the contact of albumen enzyme-to-substrate, tobacco leaf high content of protein matter is degraded to amino acid, and amino acid can generate fragrance matter with reduced sugar generation mailland reaction again.The present invention is aided with the biologically active catalytic specie (being biological respinse promoter) that activates the various enzyme activity of tobacco leaf self, as pyruvic acid, tryptophan, leucine, Zn 2+, Mn 2+, Mg 2+, K +Deng, quicken the tobacco mellowing process, the alcoholization cycle was shortened to about 6 months.Utilize biologic product method of the present invention to handle tobacco leaf, not only shortened the natural alcoholization time, and the flue gas quality improves a lot, the sensory evaluating smoking thinks that the fragrance qualitative change is good, and perfume quantity increases, and especially fragrance matter is pure, strong, pleasant impression is clean, and excitant is eliminated substantially, and assorted gas alleviates.
Specific embodiments
Embodiment 1
Get 1000 gram Yunnan Flue-cured Tobacco C 3F beating and double roasting tobacco leaf, moisture is 12%, take by weighing mixed enzyme 0.6 gram, pyruvic acid 0.1 gram, tryptophan, each 0.1 gram of leucine, zinc sulfate and manganese sulfate, each 0.01 gram of magnesium carbonate, potassium tartrate 1 gram, use the 5ml dissolved in distilled water, the spraying join in the tobacco leaf, pack then and put into the warehouse among refine.Take out alcoholization handled in 6 months samples be untreated the same grade sample in 3 years of natural alcoholization respectively chopping roll the sample cigarette, equilibrium water conten is 24 hours under relative humidity 60% condition, carries out the sensory evaluating smoking.Smoking result shows that enzyme processing sample flue gas quality is better than contrast, shows as fragrance U.S. of fine quality, and the flue gas exquisiteness stimulates and assorted gas is eliminated.
Embodiment 2
Get 1000 gram Yunnan Flue-cured Tobacco B 3F beating and double roasting tobacco leaf, moisture is 11%, take by weighing mixed enzyme 1.5 grams, pyruvic acid 0.25 gram, tryptophan, each 0.2 gram of leucine, zinc sulfate and manganese sulfate, each 0.03 gram of magnesium carbonate, potassium tartrate 3 grams, use the 10ml dissolved in distilled water, the spraying join in the tobacco leaf, pack then and put into the warehouse among refine.Take out alcoholization handled in 6 months samples be untreated the same grade sample in 3 years of natural alcoholization respectively chopping roll the sample cigarette, equilibrium water conten is 24 hours under relative humidity 60% condition, carries out the sensory evaluating smoking.Smoking result shows that enzyme processing sample flue gas quality is better than contrast, shows as flue gas and becomes pure and mild, and excitant and assorted gas obviously reduce.
Embodiment 3
Get 1000 gram Yunnan Flue-cured Tobacco X 1F beating and double roasting tobacco leaf, moisture is 13%, take by weighing mixed enzyme 3 grams, pyruvic acid 0.4 gram, tryptophan, each 0.3 gram of leucine, zinc sulfate and manganese sulfate, each 0.05 gram of magnesium carbonate, potassium tartrate 5 grams, use the 10ml dissolved in distilled water, the spraying join in the tobacco leaf, pack then and put into the warehouse among refine.Take out alcoholization handled in 6 months samples be untreated the same grade sample in 3 years of natural alcoholization respectively chopping roll the sample cigarette, equilibrium water conten is 24 hours under relative humidity 60% condition, carries out the sensory evaluating smoking.Smoking result shows that enzyme processing sample flue gas quality is better than contrast, and showing as fragrance concentration increases, and excitant and assorted gas obviously alleviate.
Mixed enzyme in the foregoing description is a protease and the ratio of pectase weight is 4: 1 a mixture.

Claims (6)

1, a kind of biologic enzyme treatment method of natural alcoholization of re-flue-cured tobacco that promotes is included in the mixture that adds protease and pectase in the beating and double roasting tobacco leaf; Technical scheme of the present invention can also comprise adding biochemical reaction promoter in tobacco leaf, as pyruvic acid, tryptophan, leucine and configuration metal ions Zn 2+, Mn 2+, Mg 2+And K +
After the above-mentioned redrying blade packing of having added enzyme and biochemical reaction promoter, be deposited in the tobacco mellowing warehouse, under the natural alcoholization condition, stored 3~12 months.
2, the biologic enzyme treatment method of promotion natural alcoholization of re-flue-cured tobacco according to claim 1, it is characterized in that, the ratio that adds protease and pectase in the beating and double roasting tobacco leaf is 4: 1 a mixture, and this mixture adding proportion is to account for 0.06~0.3% of tobacco leaf weight; Can also adding proportion be the pyruvic acid of tobacco leaf weight 0.01~0.04% in tobacco leaf, adding proportion be tryptophan, the leucine of tobacco leaf weight 0.01~0.03%, and adding proportion is the configuration metal ions Zn of tobacco leaf weight 0.001~0.005% 2+, Mn 2+, Mg 2+, Zn 2+, Mn 2+, Mg 2+Ratio be 1: 1: 1, adding proportion is the K of tobacco leaf weight 0.1~0.5% +
3, the biologic enzyme treatment method of promotion natural alcoholization of re-flue-cured tobacco according to claim 1 and 2 is characterized in that, natural alcoholization storage period is about 6 months.
4, the biologic enzyme treatment method of promotion natural alcoholization of re-flue-cured tobacco according to claim 1 and 2 is characterized in that, gets 1000 gram Yunnan Flue-cured Tobacco C 3F beating and double roasting tobacco leaf, moisture is 12%, take by weighing mixed enzyme 0.6 gram, pyruvic acid 0.1 gram, tryptophan, each 0.1 gram of leucine, zinc sulfate and manganese sulfate, each 0.01 gram of magnesium carbonate, potassium tartrate 1 gram, use the 5ml dissolved in distilled water, the spraying join in the tobacco leaf, pack then and put into the warehouse among refine.
5, the biologic enzyme treatment method of promotion natural alcoholization of re-flue-cured tobacco according to claim 1 and 2 is characterized in that, gets 1000 gram Yunnan Flue-cured Tobacco B 3F beating and double roasting tobacco leaf, moisture is 11%, take by weighing mixed enzyme 1.5 grams, pyruvic acid 0.25 gram, tryptophan, each 0.2 gram of leucine, zinc sulfate and manganese sulfate, each 0.03 gram of magnesium carbonate, potassium tartrate 3 grams, use the 10ml dissolved in distilled water, the spraying join in the tobacco leaf, pack then and put into the warehouse among refine.
6, the biologic enzyme treatment method of promotion natural alcoholization of re-flue-cured tobacco according to claim 1 and 2 is characterized in that, gets 1000 gram Yunnan Flue-cured Tobacco X 1F beating and double roasting tobacco leaf, moisture is 13%, take by weighing mixed enzyme 3 grams, pyruvic acid 0.4 gram, tryptophan, each 0.3 gram of leucine, zinc sulfate and manganese sulfate, each 0.05 gram of magnesium carbonate, potassium tartrate 5 grams, use the 10ml dissolved in distilled water, the spraying join in the tobacco leaf, pack then and put into the warehouse among refine.
CNB021165947A 2002-04-12 2002-04-12 Biologic enzyme treatment method for promoting natural alcoholization of re-flue-cured tobacco Expired - Lifetime CN1194642C (en)

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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100525661C (en) * 2004-11-12 2009-08-12 上海爱普香料有限公司 Tobacco ageing additive liquid
CN102018278A (en) * 2010-11-24 2011-04-20 华宝食用香精香料(上海)有限公司 Method of reducing protein content in cut tobacco by enzymatic treatment
CN102181414A (en) * 2011-02-23 2011-09-14 红云红河烟草(集团)有限责任公司 Complex enzyme additive for cigarettes as well as preparation method and application thereof
CN101496636B (en) * 2008-01-30 2012-02-29 湖北中烟工业有限责任公司 Method for preparing and using functional plant stuffing for thin sheet raw material of paper-making process
CN101766327B (en) * 2008-12-26 2012-07-18 湖北中烟工业有限责任公司 Extraction method in the tobacco thin slice production of paper process
CN102599644A (en) * 2012-03-12 2012-07-25 红云红河烟草(集团)有限责任公司 Alcoholization method for improving tobacco leaf quality
CN101999751B (en) * 2009-09-02 2012-12-05 湖北中烟工业有限责任公司 Method for comprehensively utilizing burdock root in tobacco
CN101564201B (en) * 2008-04-21 2013-05-22 湖北中烟工业有限责任公司 Method for improving quality of tobacco leaves by enzyme treatment in tobacco shred making processing technology
CN104886762A (en) * 2015-05-22 2015-09-09 云南中烟工业有限责任公司 Alcoholization assistant agent capable of improving quality of tobacco leaves and application method thereof
CN105029684A (en) * 2015-06-25 2015-11-11 湖北中烟工业有限责任公司 Method for preparing cigar tobacco leaves by virtue of compound amino acid fermentation
CN109280580A (en) * 2018-09-13 2019-01-29 云南中烟工业有限责任公司 A kind of tealeaves baking Studies of The Aromatic Substances and the preparation method and application thereof
CN110495630A (en) * 2019-08-06 2019-11-26 河南中烟工业有限责任公司 Gas rushes the processing method of tobacco leaf

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100525661C (en) * 2004-11-12 2009-08-12 上海爱普香料有限公司 Tobacco ageing additive liquid
CN101496636B (en) * 2008-01-30 2012-02-29 湖北中烟工业有限责任公司 Method for preparing and using functional plant stuffing for thin sheet raw material of paper-making process
CN101564201B (en) * 2008-04-21 2013-05-22 湖北中烟工业有限责任公司 Method for improving quality of tobacco leaves by enzyme treatment in tobacco shred making processing technology
CN101766327B (en) * 2008-12-26 2012-07-18 湖北中烟工业有限责任公司 Extraction method in the tobacco thin slice production of paper process
CN101999751B (en) * 2009-09-02 2012-12-05 湖北中烟工业有限责任公司 Method for comprehensively utilizing burdock root in tobacco
CN102018278B (en) * 2010-11-24 2013-04-03 华宝食用香精香料(上海)有限公司 Method of reducing protein content in cut tobacco by enzymatic treatment
CN102018278A (en) * 2010-11-24 2011-04-20 华宝食用香精香料(上海)有限公司 Method of reducing protein content in cut tobacco by enzymatic treatment
CN102181414B (en) * 2011-02-23 2012-10-17 红云红河烟草(集团)有限责任公司 Complex enzyme additive for cigarettes as well as preparation method and application thereof
CN102181414A (en) * 2011-02-23 2011-09-14 红云红河烟草(集团)有限责任公司 Complex enzyme additive for cigarettes as well as preparation method and application thereof
CN102599644A (en) * 2012-03-12 2012-07-25 红云红河烟草(集团)有限责任公司 Alcoholization method for improving tobacco leaf quality
CN104886762A (en) * 2015-05-22 2015-09-09 云南中烟工业有限责任公司 Alcoholization assistant agent capable of improving quality of tobacco leaves and application method thereof
CN105029684A (en) * 2015-06-25 2015-11-11 湖北中烟工业有限责任公司 Method for preparing cigar tobacco leaves by virtue of compound amino acid fermentation
CN105029684B (en) * 2015-06-25 2016-09-21 湖北中烟工业有限责任公司 Utilize the method that compounded amino acid fermentation prepares cigar tobacco
CN109280580A (en) * 2018-09-13 2019-01-29 云南中烟工业有限责任公司 A kind of tealeaves baking Studies of The Aromatic Substances and the preparation method and application thereof
CN110495630A (en) * 2019-08-06 2019-11-26 河南中烟工业有限责任公司 Gas rushes the processing method of tobacco leaf
CN110495630B (en) * 2019-08-06 2022-02-25 河南中烟工业有限责任公司 Treatment method of air-washed tobacco leaves

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Effective date of registration: 20090227

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