CN1428074A - Quick propagation method of promising strain line of tea - Google Patents

Abstract

The quick-propagation of high-quality strain of tea includes the following steps: using new growth of plant as explant, firstly, cutting new growth of tea tree into segment, then disinfecting by using 0.1% chloromercury, rising with sterile water, inocualting the disinfected new growth into prepared culture medium, culturing under the conditions of that temp. is 20-28 deg.C, light intensity is 1500-3000 Lx and light duration of each day is 10-16 hr. to make lateral bud produce new growth, repeating the above-mentioned operation every 30-40 days so as to implement quick propagation.

Description

A kind of method for quickly breeding of promising strain line of tea

Technical field:

The present invention relates to the plant seedling breeding, more specifically relate to a kind of method for quickly breeding of promising strain line of tea.

Background technology:

Tealeaves belongs to Theaceae Camellia plant, comes from China, existing more than 3000 year of the history of among the people kind of tea of China, tea making.Now the world mainly produce genetic resources, cultivation step, the tea making technology of tea country and the custom of drinking tea etc. all be pass directly or indirectly from China.

Cuttage and two kinds of methods of planting seed are generally adopted in the breeding of tea tree.Cottage propagation is the main method of tealeaves breeding, is exactly clip tea tree branch, under certain conditions, is inserted in soil, sand or other matrix, makes it take root, come into leaves, and becomes complete and independent new plant.But this method, required vegetable material is a lot, the maternal plant material seldom the time its reproduction speed too slow.Use seminal propagation, the offspring separates, and can not keep maternal good characteristic.

Plant tissue culture technique belongs to biological technical field, and it is a totipotency of utilizing plant cell, and the little block organization of cultured in vitro plant carries out the industrial breeding technique of plant Clonal Rapid Propagation.It only needs plant tissue material seldom, and reproduction speed is fast, is not subject to seasonal restrictions.Under maternal plant situation seldom, can obtain a large amount of high quality seedlings in a short time with this method.

Summary of the invention:

The objective of the invention is, a kind of method for quickly breeding of promising strain line of tea is provided, this method is simple, easy to operate, reproduction speed is fast, can accelerate the quick breeding of tealeaves new varieties fine individual plant and the update of local tea variety.

In order to achieve the above object, the present invention adopts following technical measures: with the tea tree young sprout as explant, young sprout is cut into 2～3 centimetres stem section, with 0.1% chlorine mercury sterilization 5～15 minutes, with aseptic water washing 3～4 times, the young sprout that disinfects is inserted in the prepared culture medium, this medium is made up of MS minimal medium and added substance, illumination cultivation, in temperature is 20～28 ℃, and light intensity 1500～3000Lx cultivates under 10～16 hours the condition of illumination every day, make lateral bud grow young sprout, repeated above-mentioned other step of removing sterilization and flushing every 30～40 days and expand numerous.

The present invention compared with prior art has the following advantages and effect: easy to implement the method, easy to operate, reproduction speed is fast, and 30～40 days propagation multiple reaches 3～4 times, and a bud can be bred 1,000,000 strain seedlings in 1 year.

Embodiment:

The specific embodiment of the present invention is: as outer plant corpus, at first young sprout is cut into 2～3 centimetres stem section with plant tea tree young sprout, with 0.1% chlorine mercury sterilization 5～15 minutes, uses aseptic water washing 3～4 times; Then the young sprout that disinfects is inserted in the prepared culture medium, this medium is made up of MS minimal medium and added substance, and wherein the composition of added substance is that BA0.5～5mg/L, NAA0.01～0.5mg/L, agar 6000～8000mg/L, sugar 20000～30000mg/L are mixed; The 3rd is illumination cultivation, is 20～28 ℃ in temperature, light intensity 1500～3000Lx, and illumination every day was cultivated under the condition in 10～16 hours, made lateral bud grow young sprout, repeated above-mentioned other step of removing sterilization and flushing every 30～40 days and expanded numerous.

The composition of MS minimal medium be (unit: mg/L):

NH ₄NO ₃ 1650

KNO ₃ 1900

CaCl ₂·2H ₂O 440

MgSO ₄·7H ₂O 370

KH ₂PO ₄ 170

KI 0.83

H ₃BO ₃ 6.2

MnSO ₄·4H ₂O 22.3

ZnSO ₄·7H ₂O 8.6

Na ₂MoO ₄·2H ₂O 0.25

CuSO ₄·5H ₂O 0.025

CoCl ₂·6H ₂O 0.025

FeSO ₄·7H ₂O 27.8

Na ₂·EDTA·2H ₂O 37.3

Inositol 100

Nicotinic acid 0.5

Puridoxine hydrochloride 0.5

Thiamine hydrochloride 0.1

Glycine 2

Claims (2)

1, a kind of method for quickly breeding of promising strain line of tea, this method follow these steps to order to carry out:

A, the tea tree young sprout is cut into the stem section;

B, with 0.1% chlorine mercury sterilization, use aseptic water washing;

C, young sprout stem section is inserted in the prepared culture medium;

D, be 20～28 ℃ in temperature, light intensity 1500～3000Lx cultivates under 10～16 hours the condition of illumination every day;

E, every 30～40 days, the step that repeats A, C, D expands numerous.

2, the method for quickly breeding of a kind of promising strain line of tea according to claim 1, it is characterized in that, described medium is made up of MS minimal medium and added substance, and wherein: the composition of added substance is BA0.5～5mg/L, NAA0.01～0.5mg/L, agar 6000～8000mg/L, sugar 20000～30000mg/L.