CN1425760A - Fungus and method for producing chitosanase with it - Google Patents
Fungus and method for producing chitosanase with it Download PDFInfo
- Publication number
- CN1425760A CN1425760A CN 01135245 CN01135245A CN1425760A CN 1425760 A CN1425760 A CN 1425760A CN 01135245 CN01135245 CN 01135245 CN 01135245 A CN01135245 A CN 01135245A CN 1425760 A CN1425760 A CN 1425760A
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- Prior art keywords
- paecilomyces
- chitoanase
- fungi
- fungus
- potato
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- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The fungus is Paecilomyces sp.JW1727, preserved in CCTCC in the preservation number of 200025. When it is used in prdoucing chitosanase, the fungus strain is first activated with potato medium and then inoculated to liquid potato culture medium containing chitosan and shaking cultured, and finally the thallus is filtered out. The present invention has the advantages of easy-to-culture fungus strain, short chitosanse producing period and high enzyme activity, is favorable to the development and utilization of chitosanase.
Description
The present invention relates to a kind of fungi, particularly relate to a kind of Paecilomyces sp.JW1727 and produce the method for chitoanase with it.
According to the applicant by known to the literature search, the microorganism that can produce chitoanase has muscardine (Beauveria bassiana), Penicillium notatum (Penicillium), pseudomonas (Pseudomonassp.) etc., the shortcoming of producing chitoanase with them is a complicated condition, inulinase-producing activity is low, has influenced the development and application of chitoanase.Up to now, there is not relevant Paecilomycessp.JW1727 and produce the relevant report of chitoanase with its.
The purpose of this invention is to provide a kind of fungi Paecilomyces sp.JW1727 and produce the method for chitoanase, can overcome the above-mentioned shortcoming of prior art with it.
A kind of fungi is characterized in that this fungi is Paecilomyces sp.JW1727, and depositary institution is CCTCC, and preserving number is 200025.
A kind of fungi Paecilomyces sp.JW1727 is characterized in that it is used to produce chitoanase.
A kind of method of producing chitoanase with Paecilomyces sp.JW1727, it is characterized in that with bacterial classification with the potato culture activation after, this bacterial classification is inserted contain in the potato liquid nutrient medium of chitosan again, shake-flask culture removes by filter thalline then.
Advantage of the present invention is that Paecilomyces sp.JW1727 bacterial classification is cultivated easily, produces enzyme time weak point, and the enzymic activity height is produced chitoanase with it, and cultural method is simple, and the inulinase-producing activity height helps the development and utilization of chitoanase.
Below by example explanation the present invention.
Accompanying drawing is a fungi aspect graph of the present invention.
Earlier by using the selective medium potato culture in the soil of aquatic product manufactory (by potato 200g, sucrose 20g, agar 15g, water 1000ml forms, pH 7 adds 0.5% chitosan) 24 ℃ cultivate 96h and isolate Paecilomyces sp.JW1727 bacterial strain, cultivate 120h with potato culture then, the bacterium colony yellowish pink of this bacterial strain, cotton-shaped, reverse side is filbert.Mycelial growth is entangled with chain, can produce huge spore, conidium circle or oval, and bottle metulae portion expands.It is wide that this bacterial classification has the nutritional requirement scope, and the characteristics of cultivating particularly have high chitoanase activity easily, and its fermentation broth enzyme vigor is 580U/ml.
Behind actication of culture, the inoculum size with 5% inserts and contains in the potato liquid nutrient medium of 0.5% chitosan, at 28 ℃ of following shake-flask culture 72h, removes by filter thalline then, promptly obtains chitoanase liquid.This enzyme liquid is acted on 2% chitosan solution, after insulation under 30 ℃, can obtain the Chitosan poly oligosaccharide and the chitobiose of different polymerization degree.
Claims (3)
1, a kind of fungi is characterized in that this fungi is Paecilomyces sp.JW1727, and depositary institution is CCTCC, and preserving number is 200025.
2, a kind of fungi Paecilomyces sp.JW1727 is characterized in that it is used to produce chitoanase.
3, a kind of method of producing chitoanase with Paecilomyces sp.JW1727, it is characterized in that with bacterial classification with the potato culture activation after, this bacterial classification is inserted contain in the potato liquid nutrient medium of chitosan again, shake-flask culture removes by filter thalline then.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01135245 CN1425760A (en) | 2001-12-12 | 2001-12-12 | Fungus and method for producing chitosanase with it |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01135245 CN1425760A (en) | 2001-12-12 | 2001-12-12 | Fungus and method for producing chitosanase with it |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1425760A true CN1425760A (en) | 2003-06-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01135245 Pending CN1425760A (en) | 2001-12-12 | 2001-12-12 | Fungus and method for producing chitosanase with it |
Country Status (1)
| Country | Link |
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| CN (1) | CN1425760A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1300311C (en) * | 2005-06-14 | 2007-02-14 | 浙江大学 | Preparation method of chitin incision enzyme |
-
2001
- 2001-12-12 CN CN 01135245 patent/CN1425760A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1300311C (en) * | 2005-06-14 | 2007-02-14 | 浙江大学 | Preparation method of chitin incision enzyme |
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
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