CN1397636A - Fungus and process for preparing chitosanase fram it - Google Patents
Fungus and process for preparing chitosanase fram it Download PDFInfo
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- CN1397636A CN1397636A CN 02135192 CN02135192A CN1397636A CN 1397636 A CN1397636 A CN 1397636A CN 02135192 CN02135192 CN 02135192 CN 02135192 A CN02135192 A CN 02135192A CN 1397636 A CN1397636 A CN 1397636A
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- chitoanase
- paecilomyces
- chitosanase
- fungi
- potato
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Abstract
A fungus Paeciomyces sp.JW1727 (CCTCC, No.200025) is disclosed. It can be used to prepare chitosanase through activating with potato culture medium, inocualting it to liquid potato cultare medium containing chitosan, shake-flask culturing and filtering. Its advantages are simple process, short period and high activity of chitosanase.
Description
The present invention relates to a kind of fungi, particularly relate to a kind of Paecilomyces sp.JW1727 and produce the method for chitoanase with it.
By known to the literature search, the microorganism that can produce chitoanase has muscardine (Beauveria bassiana), Penicillium notatum (Penicillium), pseudomonas (Pseudomonassp.) Aspergillrs sp.Yzk, B.gladioli CHB101 and Paecilomyces lilacinus (Paecilomyceslilacinus) to produce the report of chitin matter enzyme according to the applicant.Difference Paecilomyces sp.Jw1727 according to morphological specificity and product enzyme feature is non-Paecilomyces lilacinus, this bacterial classification has been obeyed shortcoming that the product enzyme time is long or enzymic activity is low of in the past producing chitoanase etc., the shortcoming of producing chitoanase with them is a complicated condition, inulinase-producing activity is low, has influenced the development and application of chitoanase.Up to now, there is not relevant Paecilomyces sp.JW1727 and produce the relevant report of chitoanase with its.
The purpose of this invention is to provide a kind of fungi Paecilomyces sp.JW1727 and produce the method for chitoanase, can overcome the above-mentioned shortcoming of prior art with it.
A kind of fungi is characterized in that this fungi is Paecilomyces sp.JW1727, and depositary institution is CCTCC, and preserving number is 200025.
A kind of fungi Paecilomyces sp.JW1727 is characterized in that it is used to produce chitoanase.
A kind of method of producing chitoanase with Paecilomyces sp.JW1727, it is characterized in that with bacterial classification with the potato culture activation after, this bacterial classification is inserted contain in the potato liquid nutrient medium of chitosan again, shake-flask culture removes by filter thalline then.
Advantage of the present invention is that Paecilomyces sp.JW1727 bacterial classification is cultivated easily, produces chitoanase with it, and cultural method is simple, and the inulinase-producing activity height helps the development and utilization of chitoanase.
Below by example explanation the present invention.
Accompanying drawing is a fungi aspect graph of the present invention.
Earlier by using the selective medium potato culture in the soil of aquatic product manufactory (by potato 200g, sucrose 20g, agar 15g, water 1000ml forms, and pH is 7, adds 0.5% chitosan) 24 ℃ cultivate 96h and isolate Paecilomyces sp.JW1727 bacterial strain, cultivate 120h with potato culture then, the bacterium colony yellowish pink of this bacterial strain, cotton-shaped, reverse side is filbert.Mycelial growth is entangled with chain, can produce huge spore, conidium circle or oval, and bottle metulae portion expands.It is wide that this bacterial classification has the nutritional requirement scope, and the characteristics of cultivating particularly have high chitoanase activity easily, and its fermentation broth enzyme vigor is 580U/ml.
Behind actication of culture, the inoculum size with 5% inserts and contains in the potato liquid nutrient medium of 0.5% chitosan, at 28 ℃ of following shake-flask culture 72h, removes by filter thalline then, promptly obtains chitoanase liquid.This enzyme liquid is acted on 2% chitosan solution, after insulation under 30 ℃, can obtain the Chitosan poly oligosaccharide and the chitobiose of different polymerization degree.
Claims (3)
1, a kind of fungi is characterized in that this fungi is Paecilomyces sp.JW1727, and depositary institution is CCTCC, and preserving number is 200025.
2, a kind of fungi Paecilomyces sp.JW1727 is characterized in that it is used to produce chitoanase.
3, a kind of method of producing chitoanase with Paecilomyces sp.JW1727, it is characterized in that with bacterial classification with the potato culture activation after, this bacterial classification is inserted contain in the potato liquid nutrient medium of chitosan again, shake-flask culture removes by filter thalline then.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02135192 CN1397636A (en) | 2002-07-11 | 2002-07-11 | Fungus and process for preparing chitosanase fram it |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02135192 CN1397636A (en) | 2002-07-11 | 2002-07-11 | Fungus and process for preparing chitosanase fram it |
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| Publication Number | Publication Date |
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| CN1397636A true CN1397636A (en) | 2003-02-19 |
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| CN 02135192 Pending CN1397636A (en) | 2002-07-11 | 2002-07-11 | Fungus and process for preparing chitosanase fram it |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1300311C (en) * | 2005-06-14 | 2007-02-14 | 浙江大学 | Preparation method of chitin incision enzyme |
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2002
- 2002-07-11 CN CN 02135192 patent/CN1397636A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1300311C (en) * | 2005-06-14 | 2007-02-14 | 浙江大学 | Preparation method of chitin incision enzyme |
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