CN1391869A - Method for monitoring micro circulation blood flow time-space response characteristic on mesentery by using laser speckle imaging instrument - Google Patents
Method for monitoring micro circulation blood flow time-space response characteristic on mesentery by using laser speckle imaging instrument Download PDFInfo
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- CN1391869A CN1391869A CN 02138787 CN02138787A CN1391869A CN 1391869 A CN1391869 A CN 1391869A CN 02138787 CN02138787 CN 02138787 CN 02138787 A CN02138787 A CN 02138787A CN 1391869 A CN1391869 A CN 1391869A
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Abstract
The equipment for monitoring micro circulation body flow time-space response characteristic on mesentery includes an optical path and an imaging system. In the optial path, the laser bean from helium neon laser is coupled to fiber bundle to form homogeneous diffused beam; and the imaging system consists of stereo microscope with CCD camera, image collecting card with image collecting and controlling software. The CCD signal is output to the image collecting card connected to PC, the collection parameters are set via the image collecting and controlling software and local blood flow information are obtained with the image signal and via the signal analysis software. The equipment provides one new way to the research of influence of medicine and other stimulation to the micro circulation blood flow on mesentery.
Description
Technical field
The present invention relates to a kind of method of utilizing microcirculation blood flow spatio-temporal response characteristic on the laser speckle imager monitoring mesentery, be specially adapted to utilize on the reflective formation method research mesentery microcirculation at the flow velocity of medicine and other stimulation lower area blood flow, caliber, changes in flow rate.
Background technology
At present, explore the research of medicine in a large number, utilize the mesenteric mesaraic transparency live body microcirculation influence, with it as microcirculatory observation sample.In these researchs, adopt means such as optical microscope, micro tv video recording system and laser-Doppler microscope that the microcirculation parameter on the mesentery is monitored.These methods are owing to adopt transmission mode to measure, therefore measuring object can only be transparent reasonable blood capillary, and can not measure for the relatively poor blood vessel of the transparency, and because the characteristics of measuring method, can only measure the blood flow in the single vessel at every turn, be not easy to analyze the spatio-temporal response characteristic of microcirculation blood flow.
Summary of the invention
The object of the present invention is to provide a kind of Real-time and Dynamic, with the method for high time space resolution ground monitoring at the spatio-temporal response characteristic of the regional blood flow of microcirculation on the body mesentery (comprising the blood vessel that the transparency is relatively poor) under medicine or other stimulus effects condition.
The present invention relates to a kind of method of utilizing microcirculation blood flow spatio-temporal response characteristic on the laser speckle imager monitoring mesentery, it comprises light path and imaging system.The light beam coupling that optical routing helium-neon (He-Ne) laser instrument sends forms even diffusion light beam to fibre bundle, the diffusion light beam be radiated on the monitored sample; Imaging system is made of stereoscopic microscope, image pick-up card and image acquisition control software, the signal analysis software of charged sub-coupled apparatus (CCD) camera.By the optical signal of the stereoscopic microscope collection of being with the CCD camera by monitored sample reflection, and its signal outputed to the image pick-up card that is attached thereto, image pick-up card is connected with computer, by the control of the image acquisition in computer software set acquisition parameter, as: image pixel size, number of image frames, time of exposure and sample frequency are come the images acquired signal, picture signal is handled by image analysis software and is obtained regional velocity flow profile information, and shows on the output device of computer or the output monitoring result.
The present invention is said to utilize the method for microcirculation blood flow spatio-temporal response characteristic on the laser speckle imager monitoring mesentery, and its advantage is: can be real-time dynamicly, with the variation of blood flow rate, blood vessels caliber and the blood flow of microcirculation blood flow under medicine and other incentive condition on high time and the spatial resolution ground monitoring mesentery.With compare with microcirculatory instrument on other existing research mesentery, it need not scanning, can once be scattered in picture to the blood flow on certain area, can change the blood flow of opaque place blood vessel and monitor, and system structure is simple, and is easy to operate, helps now with mesentery as sample, research medicine and other stimulate the research to the microcirculation blood flow influence, for the functional screening of medicine provides a kind of new approach.
Description of drawings
Fig. 1: laser speckle imaging systems figure.
Specific embodiments
The present invention includes light path and imaging system for a kind of method of utilizing microcirculation blood flow spatio-temporal response characteristic on the laser speckle imager monitoring mesentery.Optical routing He-Ne laser instrument, fibre bundle and three-dimensional adjustable being configured to; Imaging system is made of stereoscopic microscope, image pick-up card and image acquisition control software, the signal analysis software of band CCD camera.Its method is the light beam coupling sent by the He-Ne laser instrument end to fibre bundle, the fibre bundle other end adopts three-dimensional adjustable to fix, the light beam that comes out from fibre bundle forms an even diffusion light beam, illuminate zone to be monitored, optical signals CCD camera imaging, output to image pick-up card, image acquisition clamping computer comes the images acquired signal by image acquisition control software set acquisition parameter (image pixel size, number of image frames, time of exposure and sample frequency).Wherein regulate three-dimensional adjustable during imaging and make the diffusion laser beam evenly illuminate zone to be monitored, time of exposure is set at 15-20ms.
Velocity flow profile is calculated process: the CCD camera obtains original speckle pattern, the speckle that calculates per 5 * 5 pixel areas in the pattern contrast the K value (
, in the formula, K represents contrasting of speckle, σ
sRepresent the mean standard deviation of light intensity,＜I〉represent average intensity), the K value that calculates composed give each regional center pixel point, so the whole zone of searching loop obtains speckle at last and contrasts figure.Owing to contrast
, τ here
cBe correlation time,
, v is an average speed, k
0Be wave number, a is the factor that depends on the Lorentzian dispersion of distribution and tissue scatter's characteristic, and T is CCD camera exposure time (getting T usually is 15-20ms), and contrasting between figure and the flow velocity of acquisition is not proportional relation, for the purpose of directly perceived, the K value that will contrast every some correspondence among the figure is tried to achieve by following formula (1)
Value, promptly obtain velocity profile.For the variation that will stimulate the front and back blood flow shows more intuitively, we will stimulate the back and stimulate preceding velocity profile corresponding pixel point to be divided by, acquisition relative velocity variation diagram.The aforementioned calculation process is all handled by image analysis software, and end product is exported on output device.
Claims (3)
1. method of utilizing microcirculation blood flow spatio-temporal response characteristic on the laser speckle imager monitoring mesentery, include light path and imaging system, it is characterized in that, the light beam coupling that optical routing helium-neon (He-Ne) laser instrument sends forms even diffusion light beam to fibre bundle, and the diffusion light beam is radiated on the monitored sample; Imaging system is made of stereoscopic microscope, image pick-up card and image acquisition control software, the signal analysis software of charged sub-coupled apparatus (CCD) camera; Stereoscopic microscope acquired signal on monitored sample by charged sub-coupled apparatus (CCD) camera, and output to the image pick-up card that is attached thereto, image pick-up card is connected with computer, by the control of the image acquisition in computer software set acquisition parameter, picture signal is handled by image analysis software and is obtained regional velocity flow profile information, finally exports monitoring result on output device.
2. the method for microcirculation blood flow spatio-temporal response characteristic on the monitoring mesentery as claimed in claim 1, its feature is to utilize on the reflective formation method research mesentery microcirculation in the flow velocity of medicine and other stimulation lower area blood flow, caliber, changes in flow rate.
3. the method for microcirculation blood flow spatio-temporal response characteristic on the monitoring mesentery described in claim 2 is characterized in that, obtains velocity flow profile information from original speckle signals, and obtains blood vessel diameter and changes in flow rate information.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB021387877A CN1190165C (en) | 2002-07-12 | 2002-07-12 | Method for monitoring micro circulation blood flow time-space response characteristic on mesentery by using laser speckle imaging instrument |
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| CNB021387877A CN1190165C (en) | 2002-07-12 | 2002-07-12 | Method for monitoring micro circulation blood flow time-space response characteristic on mesentery by using laser speckle imaging instrument |
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| CN1190165C CN1190165C (en) | 2005-02-23 |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101919686A (en) * | 2010-09-21 | 2010-12-22 | 华中科技大学 | Multi-mode imaging system for observing cerebral cortex functions of moving animals |
| CN104873185A (en) * | 2015-06-10 | 2015-09-02 | 上海大学 | System and method for detecting skin burn injury based on near-infrared laser diffusion spectrum detection |
| CN105433906A (en) * | 2015-12-14 | 2016-03-30 | 华中科技大学 | Scanning dark field laser speckle blood flow imaging method and device |
| CN107773217A (en) * | 2017-09-29 | 2018-03-09 | 天津大学 | Biological tissue microcirculation is metabolized dynamic measurement device and method |
| CN108478192A (en) * | 2018-04-11 | 2018-09-04 | 西安交通大学 | A kind of measuring system for estimating skin histology capilary depth |
| CN109475312A (en) * | 2016-05-05 | 2019-03-15 | 康廷尤斯生物测定有限公司 | System and method for organizing to monitor and analyze |
| CN109602401A (en) * | 2019-01-28 | 2019-04-12 | 徐州市心血管病研究所 | A kind of microvascular blood flow kinetic parameter analyzer and analysis method |
| CN109717861A (en) * | 2018-12-28 | 2019-05-07 | 华中科技大学鄂州工业技术研究院 | A kind of the laser speckle detection method and detection device of blood flow velocity |
| CN109717860A (en) * | 2018-12-28 | 2019-05-07 | 华中科技大学鄂州工业技术研究院 | A kind of laser speckle blood flow velocity change detecting method and its device |
-
2002
- 2002-07-12 CN CNB021387877A patent/CN1190165C/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101919686A (en) * | 2010-09-21 | 2010-12-22 | 华中科技大学 | Multi-mode imaging system for observing cerebral cortex functions of moving animals |
| CN104873185A (en) * | 2015-06-10 | 2015-09-02 | 上海大学 | System and method for detecting skin burn injury based on near-infrared laser diffusion spectrum detection |
| CN105433906A (en) * | 2015-12-14 | 2016-03-30 | 华中科技大学 | Scanning dark field laser speckle blood flow imaging method and device |
| CN109475312A (en) * | 2016-05-05 | 2019-03-15 | 康廷尤斯生物测定有限公司 | System and method for organizing to monitor and analyze |
| CN107773217A (en) * | 2017-09-29 | 2018-03-09 | 天津大学 | Biological tissue microcirculation is metabolized dynamic measurement device and method |
| CN108478192A (en) * | 2018-04-11 | 2018-09-04 | 西安交通大学 | A kind of measuring system for estimating skin histology capilary depth |
| CN109717861A (en) * | 2018-12-28 | 2019-05-07 | 华中科技大学鄂州工业技术研究院 | A kind of the laser speckle detection method and detection device of blood flow velocity |
| CN109717860A (en) * | 2018-12-28 | 2019-05-07 | 华中科技大学鄂州工业技术研究院 | A kind of laser speckle blood flow velocity change detecting method and its device |
| CN109602401A (en) * | 2019-01-28 | 2019-04-12 | 徐州市心血管病研究所 | A kind of microvascular blood flow kinetic parameter analyzer and analysis method |
| CN109602401B (en) * | 2019-01-28 | 2021-08-27 | 徐州市心血管病研究所 | Microvascular hemodynamic parameter analyzer and analysis method |
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| CN1190165C (en) | 2005-02-23 |
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