CN1345589A - 含嗜酸乳杆菌的药物制剂及制备方法 - Google Patents
含嗜酸乳杆菌的药物制剂及制备方法 Download PDFInfo
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- CN1345589A CN1345589A CN00125469A CN00125469A CN1345589A CN 1345589 A CN1345589 A CN 1345589A CN 00125469 A CN00125469 A CN 00125469A CN 00125469 A CN00125469 A CN 00125469A CN 1345589 A CN1345589 A CN 1345589A
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- bacillus acidophilus
- preparation
- vagina
- acid
- strain
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Abstract
Description
VEC编号 | 粘附细菌数(个/VEC) | VEC编号 | 粘附细菌数(个/VEC) | VEC编号 | 粘附细菌数(个/VEC) |
1 | 132 | 18 | 92 | 35 | 85 |
2 | 113 | 19 | 120 | 36 | 123 |
3 | 74 | 20 | 89 | 37 | 144 |
4 | 149 | 21 | 65 | 38 | 131 |
5 | 186 | 22 | 78 | 39 | 156 |
6 | 118 | 23 | 158 | 40 | 98 |
7 | 111 | 24 | 75 | 41 | 143 |
8 | 63 | 25 | 126 | 42 | 125 |
9 | 84 | 26 | 118 | 43 | 95 |
10 | 89 | 27 | 109 | 44 | 194 |
11 | 94 | 28 | 138 | 45 | 88 |
12 | 118 | 29 | 80 | 46 | 155 |
13 | 119 | 30 | 128 | 47 | 178 |
14 | 125 | 31 | 74 | 48 | 122 |
15 | 90 | 32 | 77 | 49 | 167 |
16 | 120 | 33 | 50 | 50 | 133 |
17 | 129 | 34 | 62 | X±S | 113.8±33.69 |
菌株 | 大肠杆菌44113粘附数/VEC(X±S) | 白色念珠菌10231粘附数/VEC(X±S) | 统计处理 |
大肠杆菌44113酸乳杆菌SL9404+大肠杆菌44113德氏乳杆菌+大肠杆菌44113白色念珠菌10321嗜乳酸杆菌S9404+白色念珠菌10231德氏乳杆菌+白色念珠菌10231 | 157.14±54.06118.13±49.24147.78±45.12 | 31.84±11.659.76±5.9415.66±8.45 | P<0.01P>0.05P<0.01P<0.01 |
菌株 | 大肠杆菌44113粘附数/VEC(X±S) | 白色念珠菌10231粘附数/VEC(X±S) | 统计处理 |
大肠杆菌44113 | 267.22±97.80 | P<0.01 | |
嗜酸乳杆菌SL9404+大肠杆菌44113 | 125.26±51.64 | ||
白色念珠菌10231 | 29.24±8.45 | P<0.01 | |
嗜酸乳杆菌SL9404+白色念珠菌10231 | 19.54±10.24 |
菌株 | 大肠杆菌44113粘附数/VEC(X±S) | 白色念珠菌10231粘附数/VEC(X±S) | 统计处理 |
大肠杆菌44113 | 180.7±252.20 | P<0.01 | |
大肠杆菌44113+嗜酸乳杆菌SL9404 | 134.18±40.63 | ||
白色念珠菌10231 | 29.24±8.85 | 0.01<P<0.05 | |
白色念珠菌10231+嗜酸乳杆菌SL9404 | 24.40±11.36 |
组别 | 不同培养时间的活菌数(CFU/ml)Log值 | ||||||
0小时 | 12小时 | 24小时 | 48小时 | 72小时 | 96小时 | ||
试验组 | SL9404+***29403 | 5.91653.3979 | 8.35023.8692 | 8.8209<1 | 8.4983<1 | 8.0253<1 | |
SL9404+大肠杆菌44113 | 5.91653.7782 | 8.25778.8727 | 8.83958.7404 | 8.9004<5 | 8.7308<1 | ||
SL9404+白色念珠菌10231 | 5.91653.7782 | 8.32017.6821 | 8.57757.8420 | 8.77457.7474 | 8.80287.8921 | 8.75597.9731 | |
对照组 | ***29403 | 3.3979 | 3.8865 | 3.6990 | <1 | <1 | |
大肠杆菌44113 | 3.7782 | 8.8882 | 8.8176 | 8.8987 | 8.4409 | ||
白色念珠菌10231 | 3.7782 | 7.8481 | 8.1614 | 8.1903 | 8.4314 | 8.2041 | |
嗜酸乳杆菌SL9404 | 5.9165 | 8.4100 | 8.7752 | 8.2455 | 7.8722 | 7.1303 |
时间(天) | 未处理组体重(g) | 赋形剂体重(g) | O.25 x 107CFU/g/只体重(g) | O.25 X 109CFU/g/只体重(g) |
DOD2D4D6D8D10D12D14 | 219.7±11.1221.8±13.7224.9±14.1230.4±12.9231.2±13.O233.2±9.96235.24±9.66238.7±10.1 | 220.2±8.2223.9±7.1229.2±6.2237.3±8.6240.4±11.1246.7±14.4250.6±14.6253.0±14.5 | 220.0±8.9228.8±5.3236.3±7.3247.9±8.7250.9±7.8259.1±8.7259.5±10.7260.9±10.8 | 219.3±7.7223.4±8.9232.8±11.7244.5±12.8250.5±14.2255.5±14.2251.O±16.3254.3±15.5 |
检查项目 | 9404株(原代) | 9404株(30代) |
生长特性 | (1)需氧或厌氧环境中均可生长(2)在MRS琼脂上生长为浅淡黄色、中央突起圆形菌落直径约0.5-1mm。 | (1)需氧或厌氧环境中均可生长(2)在MRS琼脂上生长为浅淡黄色、中央突起圆形菌落直径约0.5-1mm。 |
染色镜检 | 革兰氏染色阳性菌体呈短杆形,散在或成对,成短链排列。菌体大小约0.5-0.8μm×1.5-3μm | 革兰氏染色阳性菌体呈短杆形,散在或成对,成短链排列。菌体大小约0.5-0.8μm×1-2.5μm |
生化反应 | 阿木鼠核葡甘果半蔗麦拉 李 萄露 乳 芽伯糖糖糖糖糖糖糖糖糖糖- - - + + + + + + + | 阿木鼠核葡甘果半蔗麦拉 李 萄露 乳 芽伯糖糖糖糖糖糖糖糖糖糖- - - + + + + + + + |
纤乳蕈蜜棉松 可淀 甘山七维 二子三 溶 露梨叶二 糖糖糖 性粉 醇醇苷糖糖糖+ + + - - + - + + + | 纤乳蕈蜜棉松 可淀 甘山七维 二子三 溶 露梨叶二 糖糖糖 性粉 醇醇苷糖糖糖+ + + - - + - + + + | |
水明 硝还 牛触杨胶 酸 奶素液 盐原 凝化 固酶+ - - + - | 水明 硝还 牛触杨胶 酸 奶素液 盐原 凝化 固酶+ - - + - | |
药敏试验 | 丁胺卡那霉素 R 四环素 S庆大霉素 R 红霉素 S链霉素 R 多粘菌素 B R新霉素 R 氯霉素 S氨苄青霉素 S 痢特灵 R关孢唑啉 S 复方新诺明 R头孢呋新 S 氟哌酸 R | 丁胺卡那霉素 R 四环素 S庆大霉素 R 红霉素 S链霉素 R 多粘菌素 B R新霉素 R 氯霉素 S氨苄青霉素 S 痢特灵 R关孢唑啉 S 复方新诺明 R头孢呋新 S 氟哌酸 R |
毒性试验 | 小鼠腹腔注射1.0×109CFU活菌后,小鼠健存,体重增加。 | |
代谢产物有机酸测定 | 乳酸0.5331%乙酸0.0796% | 乳酸0.5482%乙酸0.0600% |
G+CMol% | 39.76 | 39.76 |
检查项目 | 9403 | 9405 |
生长特性 | (1)需氧或厌氧环境中均可生长(2)在MRS琼脂上生长为浅灰黄色、圆形、突起光滑菌落1-2mm大小。 | (1)需氧或厌氧环境中均可生长(2)在MRS琼脂上生长为浅淡黄色、中央突起圆形菌落约0.5-1mm大小。 |
染色镜检 | 革兰氏染色阳性菌体呈短杆形,约0.3-0.5μm×1-1.5μm。 | 革兰氏染色阳性菌体呈短杆形,约0.5-0.8μm×1-2.5μm |
生化反应 | 阿木鼠核葡甘果半蔗麦拉 李 萄露 乳 芽伯糖糖糖糖糖糖糖糖糖糖- - ± + + + + + + + | 阿木鼠核葡甘果半蔗麦拉 李 萄露 乳 芽伯糖糖糖糖糖糖糖糖糖糖+ - + + + + + + + + |
纤乳蕈蜜棉松 可淀 甘山七维 二子三 溶 露梨叶二 糖糖糖 性粉 醇醇苷糖糖糖+ + + - - + - + + + | 纤乳蕈蜜棉松 可淀 甘山七维 二子三 溶 露梨叶二 糖糖糖 性粉 醇醇苷糖糖糖+ - + + - + - + + + | |
水明 硝还 石触杨胶 酸 蕊素液 盐原 牛化 奶酶+ - - + - | 水明 硝还 牛触杨胶 酸 奶素液 盐原 凝化 固酶+ - - + - | |
药敏试验 | 丁胺卡那霉素 R 四环素 S庆大霉素 S 红霉素 S链霉素 S 多粘菌素B R新霉素 R 氯霉素 S氨苄青霉素 S 痢特灵 R关孢唑啉 S 复方新诺明 R头孢呋新 S 氟哌酸 R | 丁胺卡那霉素 R 四环素 S庆大霉素 R 红霉素 S链霉素 R 多粘菌素 B R新霉素 R 氯霉素 S氨苄青霉素 S 痢特灵 R关孢唑啉 S 复方新诺明 S头孢呋新 S 氟哌酸 R |
代谢产物有机酸测定 | 乳酸0.9106%乙酸0.0560% | 乳酸0.6884%乙酸0.1115% |
G+CMol% | 41.84 | 42.88 |
VEC编号 | 粘附细菌数(个/VEC) | VEC编号 | 粘附细菌数(个/VEC) | VEC编号 | 粘附细菌数(个/VEC) |
1 | 132 | 18 | 92 | 35 | 85 |
2 | 113 | 19 | 120 | 36 | 123 |
3 | 74 | 20 | 89 | 37 | 144 |
4 | 149 | 21 | 65 | 38 | 131 |
5 | 186 | 22 | 78 | 39 | 156 |
6 | 118 | 23 | 158 | 40 | 98 |
7 | 111 | 24 | 75 | 41 | 143 |
8 | 63 | 25 | 126 | 42 | 125 |
9 | 84 | 26 | 118 | 43 | 95 |
10 | 89 | 27 | 109 | 44 | 194 |
11 | 94 | 28 | 138 | 45 | 88 |
12 | 118 | 29 | 80 | 46 | 155 |
13 | 119 | 30 | 128 | 47 | 178 |
14 | 125 | 31 | 74 | 48 | 122 |
15 | 90 | 32 | 77 | 49 | 167 |
16 | 120 | 33 | 50 | 50 | 133 |
17 | 129 | 34 | 62 | X±S | 113.8±33.69 |
菌株 | 大肠杆菌44113粘附数/VEC(X±S) | 白色念珠菌10231粘附数/VEC(X±S) | 统计处理 |
大肠杆菌44113 | 180.7±252.20 | P<0.01 | |
大肠杆菌44113+嗜酸乳杆菌SL9404 | 134.18±40.63 | ||
白色念珠菌10231 | 29.24±8.85 | 0.01<P<0.05 | |
白色念珠菌10231+嗜酸乳杆菌SL9404 | 24.40±11.36 |
组别 | 不同培养时间的活菌数(CFU/ml)Log值 | ||||||
0小时 | 12小时 | 24小时 | 48小时 | 72小时 | 96小时 | ||
试验组 | SL9404+***29403 | 5.91653.3979 | 8.35023.8692 | 8.8209<1 | 8.4983<1 | 8.0253<1 | |
SL9404+大肠杆菌44113 | 5.91653.7782 | 8.25778.8727 | 8.83958.7404 | 8.9004<5 | 8.7308<1 | ||
SL9404+白色念珠菌10231 | 5.91653.7782 | 8.32017.6821 | 8.57757.8420 | 8.77457.7474 | 8.80287.8921 | 8.75597.9731 | |
对照组 | ***29403 | 3.3979 | 3.8865 | 3.6990 | <1 | <1 | |
大肠杆菌44113 | 3.7782 | 8.8882 | 8.8176 | 8.8987 | 8.4409 | ||
白色念珠菌10231 | 3.7782 | 7.8481 | 8.1614 | 8.1903 | 8.4314 | 8.2041 | |
嗜酸乳杆菌SL9404 | 5.9165 | 8.4100 | 8.7752 | 8.2455 | 7.8722 | 7.1303 |
冻干菌粉 | 100mg |
微晶纤维素 | 500mg |
乳糖 | 320mg |
羧甲基淀粉钠 | 50mg |
硬脂酸镁 | 20mg |
胶态化二氧化硅 | 10mg |
冻干菌粉 | 100mg |
酒石酸 | 400mg |
碳酸氢钠 | 80mg |
碳酸钙 | 60mg |
微晶纤维素 | 200mg |
乳糖 | 120mg |
胶态化二氧化硅 | 10mg |
硬脂酸镁 | 20mg |
羟丙甲纤维素 | 10mg |
冻干菌粉 | 500mg(于双铝箔小包装袋中) |
无菌生理盐水 | 10ml(于小塑料瓶) |
冻干菌粉 | 500mg |
羊毛脂 | 1g |
凡士林 | 1g |
胶态化二氧化硅 | 2.5g |
冻干菌粉 | 100mg |
PVP | 500mg |
无菌生理盐水 | 10 |
二氧化碳 | 适量 |
Claims (4)
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Cited By (11)
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JP2014503581A (ja) * | 2011-01-28 | 2014-02-13 | プロバイオティカル・ソシエタ・ペル・アチオニ | 膣感染症を処置するため膣適用において使用するための固体形態にある発泡組成物 |
CN103695442A (zh) * | 2013-12-13 | 2014-04-02 | 上海市农业科学院 | 一种源于嗜酸乳杆菌的nfsai基因及其制备方法和应用 |
CN107714672A (zh) * | 2017-09-11 | 2018-02-23 | 广州市微生物研究所 | 一种妇用***活性益生菌凝胶包囊及其制备方法 |
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