CN1321679C - Ginger and dried orange peel extracts mixture for treating cardiovascular disease - Google Patents
Ginger and dried orange peel extracts mixture for treating cardiovascular disease Download PDFInfo
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- CN1321679C CN1321679C CNB2005100347094A CN200510034709A CN1321679C CN 1321679 C CN1321679 C CN 1321679C CN B2005100347094 A CNB2005100347094 A CN B2005100347094A CN 200510034709 A CN200510034709 A CN 200510034709A CN 1321679 C CN1321679 C CN 1321679C
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Abstract
The present invention relates to a mixture of the extract of ginger and dried tangerine peel for treating cardiovascular diseases. The composition of the weight parts is 100 of ginger extract and 0.1 to 200 of dried tangerine peel extract, wherein the ginger extract is supercritical carbon dioxide extract of the root of ginger, and the dried tangerine peel extract is steam distillation extract of the mature peel of tangerine and cultivated varieties thereof. The mixture mainly contains ginger volatile oil, gingerol and dried tangerine peel volatile oil. The present invention has the functions of free radical oxidation resistance, protection of cardiac muscle cells, adjustment of blood pressure, improvement of hemorheology, coagulation resistance, thrombosis resistance, oxygen deficiency resistance, etc. The present invention uses the mixture of the extract of ginger and dried tangerine peel as active ingredients for being combined with suitable excipient, and various medicinal preparations are prepared, such as soft capsules, capsules, drop pills, tablets, granules, oral liquid and injections. The present invention is used for various cardiovascular diseases, especially for treating myocardial ischemia and atherosclerosis.
Description
Technical field
The present invention relates to a kind of cardiovascular diseases's of treatment Rhizoma Zingiberis Recens, the mixture of Pericarpium Citri Reticulatae extract.Specifically the mature peel (Pericarpium Citri Reticulatae) with zingiber (Zingiber offficinale Rosc) rhizome, rutaceae orange (Citrus reticulata Blanco) variety is the mixture of the extract of raw material, is particularly useful for that preparation resists myocardial ischemia, antithrombotic formation, blood fat reducing, blood pressure regulation, atherosclerosis related drugs.
Background technology
Cardiovascular disease is to threaten one of human major disease, although the most countries of cardiovascular death rate except that East Bloc all has decline in various degree over nearly 30 years, but it is still majority state primary cause of death of male more than 45 years old, in the women then is second cause of the death that is only second to tumor, is having a strong impact on human life expectancy and life quality.In China, along with growth in the living standard, to suffer from cardiovascular diseases crowd and continue to rise, and be rejuvenation trend, the rural area sickness rate is suffered from the patient at present and has been exceeded 100,000,000 also in obvious rising.So treatment cardiovascular diseases's medicine not only has huge market prospect, and to ensureing that human health is significant equally.
The pathologic basis of cardiovascular disease mainly is atherosclerosis (AS).Though the etiology of AS is not illustrated so far as yet, the oxidized low density lipoprotein that result of study proposed (ox-LDL) in recent years causes the etiology of AS, is accepted by most scholars.So causing relevant link is used antioxidant the process of AS strategy from oxidative stress, to prevent and treat atherosclerosis be possible fully.In fact, many cardiovascular drugses (synthetic drug) of having found so far also have the characteristic of antioxidant simultaneously except that its main pharmacological is respectively arranged, as: calcium antagonist, beta-blocker, ACE inhibitor etc.But up to the present, Shang Weiyou is the cardiovascular disease therapies medicine of main pharmacological with the antioxidation.
In cardiovascular drugs market, treatment cardiovascular diseases's medicine is growth trend gradually.The market sales revenue of the cardiovascular disease medicine of world's treatment has at present reached more than 400 hundred million dollars, occupies the first place.Wherein calcium antagonist accounts for 19.2% of the treatment cardiovascular disease medicine market share, and ACE inhibitor has accounted for 18.2%, and hypolipidemic accounts for 13%, and beta blocker has accounted for 11.8%, and other medicine accounts for about 38%.In China, national hospital cardio-cerebrovascular medication total value was above 7,000,000,000 yuan in 2004, the proportion that Chinese medicine accounts in the treatment of cardiovascular disease will further strengthen, except " DIAOXINXUE KANG ", " BUCHANG NAOXINTONG ", " FUFANG DANSHEN DIWAN " three big kinds, some new Chinese medicines also will enter painstaking effort tubing pharmaceutical market.Though be used for the treatment of the Chinese medicine of cardiovascular disease hundreds of is arranged, owing to can not solve the atherosclerosis problem on the pharmacology, and further do not break through.
Summary of the invention
The object of the present invention is to provide a kind of have free radical resisting Oxidation, removing free radical, protecting myocardial cell, adjust blood pressure, the Rhizoma Zingiberis Recens of improving the treatment cardiovascular diseases who is used for the treatment of the cardiovascular diseases of hemorheology, anticoagulation, antithrombotic formation, anoxia enduring, the mixture of Pericarpium Citri Reticulatae extract.
The parts by weight of treatment cardiovascular diseases's of the present invention Rhizoma Zingiberis Recens, the mixture of Pericarpium Citri Reticulatae extract are composed as follows:
Rhizoma Zingiberis Recens extract 100
Pericarpium Citri Reticulatae extract 0.1-200
Its optimal proportion is:
Rhizoma Zingiberis Recens extract 100
Pericarpium Citri Reticulatae extract 50-100
Described Rhizoma Zingiberis Recens extract is the supercritical carbon dioxide extraction thing of Rhizoma Zingiberis Recens rhizome, prepares by following method exsiccant raw material Rhizoma Zingiberis Recens is dropped in the extraction kettle, feeds supercritical CO
2Fluid at 15-30MPa, extracted 0.5-2 hour under 25-40 ℃; Extract enters in the knockout drum, at 8-15MPa, separates 0.5-2 hour under 40-60 ℃, obtains the Rhizoma Zingiberis Recens supercritical CO
2Extract.
Described Pericarpium Citri Reticulatae extract is the vapor distillation extract of the mature peel of Fructus Citri tangerinae and variety thereof, prepares by following method: Pericarpium Citri Reticulatae is used vapor distillation 3-8 hour, collect distillate, get the Pericarpium Citri Reticulatae volatile oil extract after the oil-water separation.
The mixture of described Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract can directly be taken, and also technology is made oral Pharmaceutical dosage forms such as soft capsule, capsule, drop pill, tablet, granule, oral liquid or the injection that meets the preparation requirement routinely.
Treatment cardiovascular diseases's of the present invention Rhizoma Zingiberis Recens, the mixture of Pericarpium Citri Reticulatae extract can be treated the medicine of diseases such as cardiovascular diseases with the preparation of other drug compatibility.
Contain Rhizoma Zingiberis Recens volatile oil and gingerol in the Rhizoma Zingiberis Recens extract: zingiberol (being (4-hydroxy-3-methoxyphenyl)ethyl methyl ketone), zingiberene, heerabolene, α-curcumene, α-and β-farnesene, linalool, cineole, aldehyde C-9, α-Borneolum Syntheticum and β-sesquiphellandrene; Gingerol (gingerol) and catabolite shogaol thereof, zingiberone.Main component in the Pericarpium Citri Reticulatae extract is a Pericarpium Citri Reticulatae volatile oil, contains limonene, australene, γ-Song Youxi etc.
Described Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture are used to have the various cardiovascular disease of treatment of following effect, especially resist myocardial ischemia, the manufacturing of antithrombotic formation, blood fat reducing, blood pressure regulation and atherosclerosis related drugs:
(1) acute myocardial ischemia that pituitrin is caused has improvement and protective effect;
(2) have and change the hemorheological effect of general blood stasis rat model;
(3) the inductive platelet aggregation of ADP there is the obvious suppression effect;
(4) the active oxygen units of raising serum has significant free radical resisting Oxidation;
(5) improve the mouse anti stress ability,
(6) acute toxicity testing shows, the mice LD of described Rhizoma Zingiberis Recens extract
50Being 4.261mL/kg, is safe in clinical application.
(7) acute toxicity testing shows, the mice LD of described Pericarpium Citri Reticulatae extract
50Being 8.5612mL/kg, is safe in clinical application.
Rhizoma Zingiberis Recens is the fresh or dry rhizome of zingiber (Zingiber officinale Rosc.), is called Rhizoma Zingiberis Recens and Rhizoma Zingiberis.The Rhizoma Zingiberis Recens property and flavor of peppery and warm, the energy relieving the exterior syndrome by diaphoresis, warming middle-JIAO to arrest vomiting, warming the lung to relieve cough is usually used in treating anemofrigid cold, nausea and vomiting etc.; The Rhizoma Zingiberis nature and flavor are hot, energy warming middle-JIAO recuperating depleted YANG, and warming the lung to resolve fluid-retention is usually used in treating Deficiency and coldness of spleen and stomach, lung cold cough etc.The complex chemical composition of Rhizoma Zingiberis Recens, not following hundred kinds, mainly contain volatile oil, gingerol, in addition, also contain dihydrogingerol, hexahydrocurcumin, γ-An Jidingsuan, aspartic acid, glutamic acid, serine, glycine etc.
Pericarpium Citri Reticulatae is the mature peel of rutaceae orange (Citrus reticulate Blanco) and variety thereof.Pericarpium Citri Reticulatae acrid in the mouth bitterness temperature, energy regulating qi-flowing for strengthening spleen, drying dampness to eliminate phlegm.Be usually used in treating stagnation of QI in spleen and stomach, cough due to damp-phlegm etc.Contain volatile oil in the Pericarpium Citri Reticulatae, main component is a limonene, also contains flavonoid glycosides material (as hesperidin) etc.
The present invention compared with prior art has following advantage:
(1) Rhizoma Zingiberis Recens and Pericarpium Citri Reticulatae are the common medicinal and edible Chinese medicine materials that is easy to get; processing technology is simple and easy; with low cost; the extract of Rhizoma Zingiberis Recens and Pericarpium Citri Reticulatae is pressed the special ratios compatibility, has clear and definite free radical resisting oxidation, protecting myocardial cell, adjust blood pressure, improves hemorheology, anticoagulation, antithrombotic form, resist myocardial ischemia and study of anti-atherogenic effect.
(2) Rhizoma Zingiberis Recens of the present invention mixes with the extract of Pericarpium Citri Reticulatae and forms, utilization modern pharmacological research result, solve the atherosclerosis problem from the antioxidative approach, opened up the new purposes of JUPITANG, and than similar Chinese medicine preparation, effective site is clear, and action target spot is clear and definite, therapeutical effect for cardiovascular disease is unquestionable, and market potential is huge.
(3) the present invention is based on Chinese medicine and modern medicine understanding and Therapeutic Principle to the cardiovascular disease etiology and pathogenesis; in conjunction with the modern pharmacological research achievement; select circulation of qi promoting, promote blood circulation, invigorate blood circulation, the Chinese medicine of blood stasis dispelling; carry out prescription by theory of Chinese medical science; with Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae is raw material; separate purification through modern separation technology; and mix; have free radical resisting oxidation, protecting myocardial cell, adjust blood pressure, improve hemorheology, multiple effects such as anticoagulation, antithrombotic formation, anoxia enduring, can be used for making various treatment cardiovascular diseasess' medicine.
Description of drawings
Fig. 1 is the Rhizoma Zingiberis Recens extract finger printing
Fig. 2 is the graphics of the chromatographic fingerprint figure of 6 batches of Rhizoma Zingiberis Recens extract samples
The GC chromatogram that three kinds of different chromatograph pillars of Fig. 3 obtain, wherein
Fig. 3-the 1st, the chromatogram that post PEG-20M obtains
Fig. 3-the 2nd, the chromatogram that post OV-1701 obtains
The specific embodiment
Embodiment 1-1: the preparation of Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture
Take by weighing 10kg dry in advance, be crushed to the following raw material Rhizoma Zingiberis Recens of granularity 1mm, drop in the extraction kettle, feed supercritical CO
2Fluid at 25MPa, extracted 1 hour under 35 ℃; Extract enters in the knockout drum, at 10MPa, separates 1 hour under 50 ℃, obtains the Rhizoma Zingiberis Recens supercritical CO
2Extract.Take by weighing Pericarpium Citri Reticulatae medicinal material coarse powder 10kg, use vapor distillation 5 hours, collect distillate, get the Pericarpium Citri Reticulatae volatile oil extract after the oil-water separation.By Rhizoma Zingiberis Recens extract: Pericarpium Citri Reticulatae extract=weight ratio was mixed in 100: 100, obtained Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture.
Embodiment 1-2: the preparation of Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture
Take by weighing 10kg dry in advance, be crushed to the following raw material Rhizoma Zingiberis Recens of granularity 1mm, drop in the extraction kettle, feed supercritical CO
2Fluid at 15MPa, extracted 0.5 hour under 40 ℃; Extract enters in the knockout drum, at 15MPa, separates 2 hours under 40 ℃, obtains the Rhizoma Zingiberis Recens supercritical CO
2Extract.
Take by weighing Pericarpium Citri Reticulatae medicinal material coarse powder 5kg, use vapor distillation 8 hours, collect distillate, get the Pericarpium Citri Reticulatae volatile oil extract after the oil-water separation.By Rhizoma Zingiberis Recens extract: Pericarpium Citri Reticulatae extract=weight ratio was mixed in 100: 50, obtained Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture.
Embodiment 1-3: the preparation of Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture
Take by weighing 10kg dry in advance, be crushed to the following raw material Rhizoma Zingiberis Recens of granularity 1mm, drop in the extraction kettle, feed supercritical CO
2Fluid at 30MPa, extracted 2 hours under 25 ℃; Extract enters in the knockout drum, at 8MPa, separates 0.5 hour under 60 ℃, obtains the Rhizoma Zingiberis Recens supercritical CO
2Extract.
Take by weighing Pericarpium Citri Reticulatae medicinal material coarse powder 10kg, use vapor distillation 3 hours, collect distillate, get the Pericarpium Citri Reticulatae volatile oil extract after the oil-water separation.By Rhizoma Zingiberis Recens extract: Pericarpium Citri Reticulatae extract=weight ratio was mixed in 100: 0.1, obtained Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture.
Embodiment 1-4: the preparation of Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture
Take by weighing 10kg dry in advance, be crushed to the following raw material Rhizoma Zingiberis Recens of granularity 1mm, drop in the extraction kettle, feed supercritical CO
2Fluid at 25MPa, extracted 1 hour under 35 ℃; Extract enters in the knockout drum, at 10MPa, separates 1 hour under 50 ℃, obtains the Rhizoma Zingiberis Recens supercritical CO
2Extract.Take by weighing Pericarpium Citri Reticulatae medicinal material coarse powder 10kg, use vapor distillation 5 hours, collect distillate, get the Pericarpium Citri Reticulatae volatile oil extract after the oil-water separation.By Rhizoma Zingiberis Recens extract: Pericarpium Citri Reticulatae extract=weight ratio was mixed in 100: 200,, obtain Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture.
Embodiment 1-5: the preparation of Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture
Take by weighing 10kg dry in advance, be crushed to the following raw material Rhizoma Zingiberis Recens of granularity 1mm, drop in the extraction kettle, feed supercritical CO
2Fluid at 25MPa, extracted 1 hour under 35 ℃; Extract enters in the knockout drum, at 10MPa, separates 1 hour under 50 ℃, obtains the Rhizoma Zingiberis Recens supercritical CO
2Extract.Take by weighing Pericarpium Citri Reticulatae medicinal material coarse powder 10kg, use vapor distillation 5 hours, collect distillate, get the Pericarpium Citri Reticulatae volatile oil extract after the oil-water separation.By Rhizoma Zingiberis Recens extract: Pericarpium Citri Reticulatae extract=weight ratio was mixed in 100: 10, obtained Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture.
Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract and mixture that following examples all adopt embodiment 1-2 to obtain are tested
Embodiment 2: Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract and mixture improve and myocardium protecting action the cardiac function that pituitrin causes rats with myocardial ischemia
(1) Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract and mixture cause the influence of ECG change to pituitrin
1 material and method
1.1 material
1.1.1 animal: the Wistar rat, male and female are usefulness all, 140～160g
1.1.2 reagent: pituitrin (lot number 010327 is valid until 030326 for the Shanghai first biochemical Pharma Inc., biochemical-pharmaceutical factory, Shanghai), pentobarbital sodium (Shanghai No. 3 Pharmaceutical Factory's lot number 861106)
1.1.3 reagent: FUFANG DANSHEN DIWAN (available from sky, Tianjin Shi Li pharmaceutical Co. Ltd), Rhizoma Zingiberis Recens extract, Pericarpium Citri Reticulatae extract, Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture (volume ratio 2: 1)
1.1.4 instrument: IM-600 type electrophysiology instrument (Japanese photoelectricity)
1.2 method
1.2.1 animal grouping: the Wistar rat, 140～160g, male, be divided into 7 groups, 10/group.The normal control group, model control group, positive drug (FUFANG DANSHEN DIWAN) matched group, Rhizoma Zingiberis Recens extract group, Pericarpium Citri Reticulatae extract group, Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture low dose group, Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture high dose group.Pre-administration 4 days.
1.2.2 administration: blank group, model group feedwater, positive drug group FUFANG DANSHEN DIWAN dosage is 8.3mg/100g; Rhizoma Zingiberis Recens extract group dosage is 24 μ l/100g, Pericarpium Citri Reticulatae extract group dosage is 12 μ l/100g, Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture low dose group dosage are 36 μ l/100g, and Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture low dose group dosage are 9 μ l/100g.
1.2.3 45min anaesthetizes with pentobarbital sodium after the administration in the 5th day, rat back of the body position is fixing, and extremity connect two and lead, when the rat electrocardiogram is stablized, the lumbar injection pituitrin, observed and recorded 0s, 5min, 15min, 30min, electrocardiogram changes during 45min, simultaneously the variation of recorded heart rate.Normal control group physiologic saline for substitute pituitrin.
1.2.4 be designated as J point, the degrees of offset that counting statistics J is ordered to what T was ordered by S point end 1/3 place with the S point.
1.2.5 experimental record condition: chart speed: 50mm/s, voltage: 0.5mv/mm,
2 results
Table 1 is Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract and mixture cause acute myocardial ischemia to pituitrin influence.
Table 1
Compare with normal group:
*P＜0.05,
*P＜0.01; Compare with model group: #p＜0.05, ##p＜0.01 is by showing as seen each time point comparison of Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture low dosage, high dose group and positive drug control group: △ p＜0.05, △ △ p＜0.01; 45min place and 30min place compare: ▲ p＜0.05, ▲ ▲ p＜0.01
When the 30min point, with positive drug (FUFANG DANSHEN DIWAN) group, Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture high dose group ST section all are raised to peak in each administration group.Recover gradually during 45min, administration group ST section does not return to the preceding level (when being 0min) of administration.Normal group ST section is also slightly raised, but and not obvious.Model group does not then have the sign of recovery during this period.
3 conclusions
Experimental result shows, Rhizoma Zingiberis Recens extract, Pericarpium Citri Reticulatae extract, Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture all have the effect that improves the myocardial ischemia symptom due to the pituitrin in various degree, and be wherein the strongest with the effect of Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture.
(2) influence that descends of Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract and mixture serum superoxide dismutases (SOD) vigor that myocardial ischemia is caused
1 material and method
1.1 material
1.1.1 animal: Wistar rat, male and female dual-purpose, 140～160g
1.1.2 reagent: pituitrin, SOD reagent detect box (available from cold spring port, Nanjing Science ﹠ Technology Center)
1.1.3 reagent: with in " embodiment 2 " (one) " 1.1.3 ".
1.1.4 survey box: SOD reagent detects box (available from cold spring port, Nanjing Science ﹠ Technology Center)
1.1.5 instrument: HP6010 visible light-ultraviolet spectrophotometer
1.2 method step
1.2.1 animal grouping: with in " embodiment 2 " (one) " 1.2.1 ".
1.2.2 medication: with in " embodiment 2 " (one) " 1.2.2 ".
1.2.3 the administration of blank group is with feedwater, the lumbar injection pituitrin changed normal saline in the 4th day; Model group is the lumbar injection pituitrin then, and is surplus with the normal control group; Positive drug control group is then with the positive medicine of FUFANG DANSHEN DIWAN.
1.2.4 the medicine preparation: medicine is prepared per 2 days once.
1.2.5 detection method: behind the 30min, carotid artery is got blood, centrifuging and taking serum after the administration in the 4th day.Pressing the test kit description detects.
1.2.6 reagent preparation: press test kit explanation reagent preparation one, two, three, four.Reagent one, two is buffer, and reagent two prepares back 4 ℃ of preservations.Reagent three is p-anilinesulfonic acid., and reagent four is hydrochloride naphthodiamide.
1.2.7 application of sample:
1) every test tube adds sample 50 μ l.
2) each test tube adds DDW 0.25ml, reagent one, reagent two each 0.1ml successively.Do blank pipe, standard curve simultaneously.
3) mixing, 37 ℃ of water-bath 30min.
4) add reagent three, reagent four each 0.5ml.
5) with each pipe mixing, put 30 ℃ of water-bath 15min.
6) water-bath is taken out, and room temperature leaves standstill 15min, and is to be checked.
7) control tube replaces sample with DDW, and all the other handle same testing sample.
8) standard curve making: the cumulative volume of standard substance and DDW is 0.3ml, and standard substance application of sample amount is respectively 0.01ml, 0.02ml, 0.05ml, 0.1ml, 0.15ml, 0.2ml, and all the other handle all identical with other groups.SOD standard curve equation: y=-0.737x+0.150; r
2=0.997
1.2.8 use visible light-UV spectrophotometer measuring OD value at the 550nm place
2 results
Table 2 is the influences to the SOD vigor of Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract and mixture
Table 2
| Group | n | The SOD vigor |
| Normal control group model matched group positive drug control group Rhizoma Zingiberis Recens extract group Pericarpium Citri Reticulatae extract group Rhizoma Zingiberis Recens, the Pericarpium Citri Reticulatae mixture hangs down agent group Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae mixing object height agent group | 10 10 10 10 10 10 10 | 0.070±0.007 0.062±0.008 0.074±0.011 * 0.068±0.008 * 0.066±0.007 * 0.070±0.007 * 0.071±0.006 * |
Compare with model group:
*P＜0.05,
*P＜0.01
By table as seen, model group SOD vigor descends, and each administration group all can improve the SOD vigor, and the effect of Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture is stronger than the effect of Rhizoma Zingiberis Recens extract and Pericarpium Citri Reticulatae extract.
3 conclusions
Rhizoma Zingiberis Recens extract, Pericarpium Citri Reticulatae extract, Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture all have the effect that improves treating myocardial ischemia damage model SOD vigor, and the effect of the treating myocardial ischemia damage of improvement is arranged, and be the strongest with the effect of Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture.
Embodiment 3: Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract and mixture are to the hemorheological influence of blood stasis rat model
1 material and method
1.1 material
1.1.1 animal: the Wistar rat, male, 150g ± 20g
1.1.2 reagent: isoproterenol hydrochloride inj (Shanghai Hefeng Pharmaceutical Co., Ltd., lot number 000703 is available from attached first hospital of Traditional Chinese Medicine University Of Guangzhou)
1.1.3 reagent: with in " embodiment 2 " (one) " 1.1.3 "
1.1.4 instrument: L-100 blood viscometer, LIANG-microcomputer show record blood plasma viscometer automatically
1.2 method
1.2.1 animal grouping: with in " embodiment 2 " (one) " 1.2.1 ".
1.2.2 medication: with in " embodiment 2 " (one) " 1.2.2 ".
1.2.3 modeling method: adopt cold stimulation in conjunction with the epinephrine modeling method, belong to cold-condensing type syndrome of blood stasis model.With 1 times of the isoproterenol of 1mg/mL dilution, pressed 0.1ml/100g subcutaneous injection (SC) every 4 hours 1 time, totally 3 times with normal saline.0～2 ℃ of frozen water cold stimulation was put into 4 minutes with rat in centre in per twice administration.Modeling was carried out 2 days altogether.
1.2.4 medication: normal group, model control group all feed water, and positive drug control group is given FUFANG DANSHEN DIWAN.Pre-administration 3 days, after the modeling, administration is 2 days again, detects in the 3rd day.Modeling not drug withdrawal simultaneously.
1.2.5 after the modeling the 3rd day, heart was got whole blood, anticoagulant heparin.
1.2.6 detection method and index: detect hemorheology index: blood viscosity, packed cell volume, erythrocyte deformability, plasma viscosity etc.
2 results
Table 3 is that Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract and mixture are to the hemorheological influence of blood stasis rat model
Table 3
| Group | n | Red cell deformability | Erythrocyte aggregation | Erythrocyte aggregation index |
| Normal group model control group positive controls ginger extract group dried orange peel extracts group ginger, the low agent group ginger of dried orange peel mixture, dried orange peel mixing object height agent group | 10 10 10 10 10 10 10 | 6.895±1.205 10.358±1.608 △△7.314±1.495 **8.825±1.314 △△**#9.088±1.384 **△△8.606±1.412 △△*8.618±1.686 △* | 10.0641±1.466 19.633±1.797 △△10.347±1.506 **11.853±1.504 △△**#11.328±1.452 **△12.028±1.421 △△**#12.518±1.653 △△**## | 1.410±0.124 1.749±0.139 △△1.476±0.129 **1.598±0.126 △**1.689±0.136 *△1.574±0.145 △*1.546±0.139 △** |
By table as seen, compare with normal group: △ p＜0.05, △ △ p＜0.01; Compare with model group:
*P＜0.05,
*P＜0.01; Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture administration group and compound Salviae Miltiorrhizae group compare: #p＜0.05 data are through the variance test of homogeneity, and each between group variable is neat.
Ischemic heart desease is often with hemorheological change.Blood sticking, 0 dense, coagulate, poly-state, make the easier generation of ischemic heart desease.As can be seen from Table 3, compare with model group, Rhizoma Zingiberis Recens extract, Pericarpium Citri Reticulatae extract, Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture all have the significance meaning to red cell deformability, aggregation, aggregate index influence, and be wherein the strongest to mix the effect of object height agent group.
3 conclusions
Experimental result shows, Rhizoma Zingiberis Recens extract, and Pericarpium Citri Reticulatae extract, Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture all have the improvement effect to blood stasis rat model hemorheology, and the effect of Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture is than Rhizoma Zingiberis Recens extract, and the effect of Pericarpium Citri Reticulatae extract is strong.
Embodiment 4: Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract and mixture are to the influence of the inductive platelet aggregation of ADP
1 material and method
1.1 material
1.1.1 material: plastic test tube, the disposable device of penetrating
1.1.2 instrument: centrifuge, blood pool instrument LBY-NJ2
1.1.3 animal: Wistar rat, male and female dual-purpose, 200g～250g
1.1.4 reagent: aspirin (available from attached first hospital of Traditional Chinese Medicine University Of Guangzhou), Rhizoma Zingiberis Recens extract, Pericarpium Citri Reticulatae extract, Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture (volume ratio 2: 1)
1.1.5 reagent: platelet aggregation derivant ADP sodium salt, isoproterenol hydrochloride inj (Shanghai Hefeng Pharmaceutical Co., Ltd., lot number 000703 is available from attached first hospital of Traditional Chinese Medicine University Of Guangzhou), sodium citrate
1.2 experimental procedure
1.2.1 animal grouping: 70 of Wistar rats, male and female dual-purpose.Be divided into 7 groups at random, 10/group, be made as the normal control group, model control group, positive drug (aspirin) matched group, Rhizoma Zingiberis Recens extract group, Pericarpium Citri Reticulatae extract group, Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture low dose group, Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture high dose group.Except that normal group, all the other all use the modeling of epinephrine frozen water.
1.2.2 dosage regimen: ig administration, normal group, modeling group are given aqueous solution, positive controls gives aspirin, Rhizoma Zingiberis Recens extract group dosage is 24 μ l/100g, Pericarpium Citri Reticulatae extract group dosage is 12 μ l/100g, Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture low dose group dosage are 36 μ l/100g, and Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture low dose group dosage are 9 μ l/100g.
1.2.3 modeling method: the epinephrine of 1mg/mL is diluted 1 times with normal saline, pressed 0.1ml/100g subcutaneous injection (SC) every 4 hours 1 time, totally 3 times.0～2 ℃ of frozen water cold stimulation was put into 4 minutes with rat in centre in per twice administration.Modeling was carried out 2 days altogether.
1.2.4 administration totally 5 days.(modeling finishes the back and continues medication 3 days).Heart is got whole blood behind the 5th day administration 1h, inserts in the anticoagulant test tube and (handles with 1% heparin).With the centrifugal 5min of 1000rpm, draw upper strata PRP, remaining blood plasma is again with the centrifugal 10min of 4000rpm, and its supernatant is platelet-poor plasma PPP (platelet poor plasma).
1.2.5 transfer PRP with PPP, make that platelet count on average is about 450,000/μ l among the PRP.(blood pool instrument LBY-NJ2 counting)
1.2.6 get PRP0.3ml in opacity tube, transfer transmittance with PPP, detect with blood pool instrument LBY-NJ2 and trace the maximum aggregation extent of platelet in 5 minutes among the PRP.
1.2.7 calculate both ratio and platelet aggregation rate.
2 results
Table 4 is influences of Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract and the inductive platelet aggregation of mixture antagonism ADP
Table 4
| Group | n | Platelet maximum agglutination rate (%) |
| Normal control group model matched group positive drug control group Rhizoma Zingiberis Recens extract group Pericarpium Citri Reticulatae extract group Rhizoma Zingiberis Recens, the Pericarpium Citri Reticulatae mixture hangs down agent group Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae mixing object height agent group | 10 10 10 10 10 10 10 | 41.98±6.471# 48.64±5.339 *## 28.50±5.323 **## 33.07±4.767 **## 34.39±5.804 **## 33.45±4.226 **## 31.88±5.083 **## |
*Compare with the normal control group: p＜0.05;
*Compare with the normal control group: p＜0.01
# and model control group compare: p＜0.05; ## and model control group compare: p＜0.01
The result shows, model control group and the more variant p of normal control group＜0.05 illustrate modeling success: each administration group and positive drug control group all have in various degree inhibitory action to the inductive platelet aggregation of ADP, with relatively equal p＜0.01 of model control group.Wherein, the effect of Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixing object height agent group is stronger than the effect of Rhizoma Zingiberis Recens extract, Pericarpium Citri Reticulatae extract.
3 conclusions
Rhizoma Zingiberis Recens extract, Pericarpium Citri Reticulatae extract, Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture all have the obvious suppression effect to the inductive platelet aggregation of ADP, and be the strongest with the effect of Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture.
Embodiment 5: Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract and mixture free radical resisting oxidation test
1 material and method
1.1 material
1.1.1 animal: male SD rat, body weight 260～300 gram, medical experiment animal field, Guangdong Province provides, the quality certification number: (the Guangdong Province State Scientific and Technological Commission is sent out) 2001A046.Feedstuff is provided by medical experiment animal field, Guangdong Province.
1.1.2 be subjected to reagent thing and preparation: Rhizoma Zingiberis Recens extract, Pericarpium Citri Reticulatae extract, Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture (volume ratio 2: 1), vitamin E (positive control drug) (Guangzhou group of stars Pharmaceutical limited company provides).Rhizoma Zingiberis Recens extract, Pericarpium Citri Reticulatae extract are made into 2.5 μ L/mL, 1.25 μ L/mL respectively with 2% tween 80, and Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture also are made into aforementioned concentration with 2% tween 80.It is standby that vitamin E is made into 3.125mg/mL concentration with 2% tween normal saline solution.
1.1.3 reagent: (bio-engineering research institute, lot number: 20011210) are built up in Nanjing to detection kit.
1.2 method
1.2.1 the animal grouping: animal is divided into 5 groups at random by body weight, 10 every group.Be made as normal control group, positive drug control group, Rhizoma Zingiberis Recens extract group, Pericarpium Citri Reticulatae extract group, Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture.
1.2.2 administration: each organizes the equal gastric infusion of experimental rat, irritates the long-pending 10mL/kg of being of body of stomach, and the normal control group is given isopyknic 2% tween 80 normal saline solution, and positive controls is given Vit E solution, successive administration 5 days, 1 time/day.
1.2.3 sampling and measure: 30min after the last administration, rat carotid artery is got blood, and 4 ℃ leave standstill, and get serum in the centrifugal 10min of 1500rpm after separating out serum, and every Guan Junyong normal saline dilution is 20 times.By kit method operation (seeing Table 5-1), measure the OD value then.
Table 5-1 is that the Fenton reaction method is measured the antioxidant radical experimental working technique
Table 5-1
| Reagent | The blank pipe of standard | Standard pipe | Control tube | Measure pipe |
| Distilled water (mL) | 0.2 | 0.1 | 0.1 | |
| 0.03％H 2O 2Standard substance are used liquid (mL) | 0.1 | |||
| Substrate is used liquid (mL) | 0.1 | 0.1 | ||
| Sample (mL) | 0.1 | |||
| Reagent three (mL) | 0.2 | 0.2 | 0.2 | 0.2 |
| Developer (mL) | 1 | 1 | 1 | 1 |
Stipulate that every milliliter of serum reacted 1 minute down at 37 ℃, made H in the reaction system
2O
2It is an active oxygen unit that concentration reduces 1mmoL/L.Result of calculation unit is a U/mL serum.Computing formula is as follows:
1.3 statistical procedures: use SPSS statistical software 10.0 editions, carry out One-WayANOVA and analyze.
2 results
The Fenton reaction is the chemical reaction of modal generation hydroxy radical.Add electron acceptor in the hydroxy radical that Fenton reaction produces, and use the gress reagent colour development, the result presents redness, its color and hydroxy radical how much proportional.Under 550nm, measure the OD value with spectrophotometer, can predict and be subjected to the inhibitory action of reagent hydroxy radical.
The result shows (seeing Table 5-2), Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture group, Pericarpium Citri Reticulatae extract group, positive drug control group be the active oxygen units of the remarkable serum of energy all, compare p＜0.01, Rhizoma Zingiberis Recens extract group and normal group more variant (p＜0.05) with the normal control group; Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture group and positive drug control group be zero difference relatively, Rhizoma Zingiberis Recens extract group, Pericarpium Citri Reticulatae extract group and positive controls more variant (p＜0.05,0.01).
Table 5-2 is the influence to the active oxygen units of Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract and mixture
Table 5-2
| Group | n | Active oxygen units (U/mL) |
| Normal control group positive drug control group Rhizoma Zingiberis Recens extract group Pericarpium Citri Reticulatae extract group Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture group | 10 10 10 10 10 | 752.06±89.80 1075.6±08.8 **843.43±75.13 *△△971.67±108.17 **△1039.8±128.4 ** |
Annotate:
*Compare p＜0.05 with the normal control group
*Compare p＜0.01 with the normal control group
△Compare p＜0.05 with positive controls
△ △Compare p＜0.01 with positive controls
3 conclusions
Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture have significant free radical resisting Oxidation, and effect is similar to Vit E; Rhizoma Zingiberis Recens extract, the equal Wheat Protein of Pericarpium Citri Reticulatae extract list, but antioxidation does not have compatibility to make good use of.
Embodiment 6: Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract and mixture are to the experimental study of mouse anti stress ability influence
1 material and method
1.1 material
1.1.1 animal: the NIH mice, body weight 18～22 gram, male and female half and half, medical experiment animal field, Guangdong Province provides, the quality certification number: (the Guangdong Province State Scientific and Technological Commission is sent out) 2001A044.Feedstuff is provided by medical experiment animal field, Guangdong Province.
1.1.2 reagent: Rhizoma Zingiberis Recens extract, Pericarpium Citri Reticulatae extract, Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture (volume ratio 2: 1)
1.2 method
1.2.1 the animal grouping: animal is divided into 4 groups at random by body weight, 12/group: be made as normal control group (normal saline), Rhizoma Zingiberis Recens extract group, Pericarpium Citri Reticulatae extract group, Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture group.
1.2.2 test method: select the swimming with a load attached to the body test method, observe Rhizoma Zingiberis Recens extract list usefulness respectively, Pericarpium Citri Reticulatae extract list usefulness and compatibility are used the influence to the animal anti-stress ability.
1.2.3 dosage: the equal gastric infusion of each experimental mice, irritate the long-pending 20ml/kg of being of body of stomach.Rhizoma Zingiberis Recens extract group dosage is 24 μ l/100g, and Pericarpium Citri Reticulatae extract group dosage is 12 μ l/100g, and Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture group dosage are 36 μ l/100g.The normal control group is irritated stomach equal-volume 2% tween 80 normal saline solution, successive administration (water) 5 days, 1 time/day.Back 30 minutes of last administration (water), each mouse tail bears a heavy burden for 10% of body weight, carries out the normal-temperature water swimming test.Observe and write down each mice swimming time.
1.3 statistical procedures
Use SPSS statistical software 10.0 editions, carry out One-Way ANOVA and analyze.
2 results
Table 6 is the influences to the mice swimming with a load attached to the body time of Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract and mixture
Table 6
| Group | n | The mice swimming with a load attached to the body time (s) |
| Normal control group Rhizoma Zingiberis Recens extract group Pericarpium Citri Reticulatae extract group Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture group | 12 12 12 12 | 59.2±15.7 57.5±21.3 97.6±20.7 ** 98.4±22.6 ** |
Annotate: compare with the normal control group,
*P＜0.05
*P＜0.01
Result of the test shows that Pericarpium Citri Reticulatae extract group and Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture group and normal control group compare, and there is significant difference (p＜0.01) the mice swimming with a load attached to the body time, and the Rhizoma Zingiberis Recens extract group is there was no significant difference then.
3 conclusions
Pericarpium Citri Reticulatae extract and Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae extract mixture have the anti-stress ability that strengthens mice significantly and antifatigue effect significantly, and the Rhizoma Zingiberis Recens extract list is with then there not being this effect.
Embodiment 7: the Rhizoma Zingiberis Recens extract acute toxicity test
1 material and method
1.1 material
1.1.1 reagent: Hager's reagent, distilled water, Tween 80
1.1.2 reagent: Rhizoma Zingiberis Recens supercritical CO
2Extract
1.1.3 animal: NIH mice (regular grade), 18～22g, male and female half and half
1.1.4 experiment condition: open systems
1.2 method
Equal fasting be can't help water 14 hours before the experiment, the ig administration, and the administration capacity is 0.3ml/10g.Feeding behind the administration 1h is dissected dead mice, the record death condition.Observed 7 days after the administration.
1.2.1 preliminary experiment: 12 mices, male Mus numbering 1～6, female Mus numbering 7～12 is divided into three groups at random.Preliminary definite three groups of dosages are respectively per 10 gram 0.2ml, 0.10ml, 0.05ml.(dosage is 1: 0.5 between group)
1.2.1.1 preliminary experiment the results are shown in Table 7-1, is oil of ginger chmice acute toxicity preliminary experiment result.
Table 7-1
| Group | Quantity | Death toll | Dosage | The result |
| Organize two group three | 4 4 4 | 4 2 1 | 0.2ml/10g 0.1ml/10g 0.05ml/10g | All dead 2 dead 1 |
1.2.1.2, can tentatively determine the minimum whole dead dosage (D of mice oil of ginger by the preliminary experiment result
m) be 0.2ml/10g, maximum all survival dose (D
n) be 0.03ml/10g.For carrying out smoothly of guaranteeing to test, with D
mEnlarge 1.5 times and be decided to be 0.3ml/10g.
1.2.2 formal experiment: 100 of NIH mices, male and female half and half are divided into 10 groups.At D
nAnd D
mScope in be divided into 10 dose gradients, determine that dosage was with 1: 0.75 (1: K) successively decrease between group.The 1st dosage is 0.3ml/10g, and the 2nd dosage is 0.225ml/10g, and the 10th dosage is 0.0225ml/10g, and being extended to the 11st dosage is 0.01689ml/10g.Concrete dosage situation sees Table 7-2.
1.2.2.1 earlier since the 2nd dosage administration, this dosage group is promptly all dead during administration, so the 1st dosage needn't be done again.
Experimental result is as table 7-2.
1.2.2.2 experiment situation: the dead basic major part of mice occurs in first and second day, and two days later, dead quantity subtracts greatly.After the 4th day, group 10 is normal with the not dead mice outward appearance action recovery of group 9; Group 7, group 8 mices recovered normal in the 5th day: feed drinking-water, and stool, activity are all seen normally; The 7th day, group 6 not dead mices recovered normal.Mice activity of appearance successively before dead reduces, and rolls up, repose, and rapid breathing, shallow, fur does not have relaxation, does not see sialorrhea, rhinorrhea phenomenon, does not see the tic phenomenon.Dissect dead back, sees: flatulence, hyperemia, the 2nd, 3 day dead mice dissected visible stool dry and hard phenomenon, oliguria (bladder sky).Pulmonary, the heart, liver,spleen,kidney outward appearance Non Apparent Abnormality.
1.2.3 one mice day in week death condition see Table 7-2, totally experiment situation sees Table 7-3.
Table 7-2 death condition of formal experiment mice day
| Group | Death toll (only) | ||||||
| The 1st day | The 2nd day | The 3rd day | The 4th day | The 5th day | The 6th day | The 7th day | |
| Organize 1 group of 2 groups of 3 groups of 4 groups of 5 groups of 6 groups of 7 groups of 8 groups of 9 groups of 10 ∑ | 10 10 7 5 6 4 2 2 2 0 48 | 0 0 3 4 3 1 1 0 0 0 12 | 0 0 0 1 1 1 0 0 0 0 3 | 0 0 0 0 0 0 0 0 0 0 0 | 0 0 0 0 0 0 0 0 0 0 0 | 0 0 0 0 0 0 0 0 0 0 0 | 0 0 0 0 0 0 0 0 0 0 0 |
Table 7-3 oil of ginger mouse experiment acute toxicity testing situation
| Group | Death toll/number of animals | Dosage (ml/kg) | Log10 dose | Calculating chart | P [notes] | P 2 |
| Organize 1 group 2 groups 3 groups 4 groups 5 groups 6 groups 7 groups 8 groups 9 groups 10 | 10/10 10/10 10/10 10/10 10/10 6/10 3/10 2/10 2/10 0/10 | 22.50 16.88 12.66 9.49 7.12 5.34 4.00 3.00 2.25 1.69 | 1.3522 1.2272 1.1023 0.9774 0.8524 0.7275 0.6025 0.4776 0.3527 0.2277 | Dm＝22.5 Xm＝1.3522 n＝10 K＝6 i＝0.1249 | 1.0 1.0 1.0 1.0 1.0 0.6 0.3 0.2 0.2 0 | 1.0 1.0 1.0 1.0 1.0 0.36 0.09 0.04 0.04 0 |
Annotate: P is a mouse death rate, P
2Square value for mortality rate
2 results
According to experiment, calculate by improvement karber's method formula:
Oil of ginger mice median lethal dose(LD 50) LD
50=4.562ml/kg
LD
5095% fiducial limit=lg
-1(0.8552 ± 1.96 * 0.0407)=5.0211～3.6158ml/kgLD
50Average fiducial limit=4.261 ± (5.0211-3.6158)/2=4.261 ± 0.7026ml/kg
Linear regression equation is: Y=5.77x-1.19
3 conclusions
The acute toxicity testing of oil of ginger shows, the LD of its mice
50Being 4.261ml/kg, being equivalent to the about 25ml/60kg of people, be equivalent to crude drug 500g, is 50～100 times of clinical usual amounts.Thus, can think tentatively that oil of ginger is safe in clinical practice.
Dissect in the experimentation and find that oil of ginger has bigger stimulation to gastrointestinal tract, causes gastrointestinal mucosa hyperemia, and large intestine has the constipation with dry stool phenomenon.Organ outward appearances such as the heart, lung, liver,spleen,kidney do not have significant change.Mice most of death about 1～2 hour in the 1st day, the 2nd day then few, do not have the phenomena of mortality substantially, then recovered normal on the 7th day in the 3rd day.
Embodiment 8: the Pericarpium Citri Reticulatae extract acute toxicity test
1 material and method
1.1 material
1.1.1 reagent: distilled water, Pericarpium Citri Reticulatae extract, Tween 80
The common mice of 1.1.2 animal: NIH, 18～22g, male and female half and half
1.1.3 experiment condition: weather is fine; Room temperature, 26 ℃～29 ℃; Relative humidity, 50%～74%
1.2 method
Undertaken by the improvement karber's method.
1.2.1 water is can't help in experiment fasting evening before that day.The ig administration, the administration capacity is decided to be 0.3ml/10g substantially, and feeding behind the administration 1h was observed 7 days.
1.2.2 carry out trial test, try to achieve D
m(minimum whole dead dosage) and D
n(maximum all survival doses).Preliminary experiment has carried out 2 times, sees Table 8-1, table 8-2.
1.2.3 preliminary experiment result: can tentatively determine D
mBe 0.15ml/10g, D
nBe 0.025ml/10g.Anatomy: be GI irritation, stomachus pyloricus hyperemia, gastrointestinal mucosa hyperemia, outward appearances such as all the other organ hearts, lung, spleen, liver, kidney are not seen significant change.
Table 8-1 Pericarpium Citri Reticulatae extract chmice acute toxicity preliminary experiment () dead mouse situation
| Group | Quantity (only) | Death toll (only) | Ig dosage (ml/10g) | The result |
| Organize 1 group 2 groups 3 groups 4 groups 5 | 4 4 4 4 4 | 3 4 4 4 4 | 0.1 0.2 0.3 0.4 0.5 | Dead 3 all dead all death are all dead all dead |
Table 8-2. Pericarpium Citri Reticulatae extract chmice acute toxicity preliminary experiment (two) dead mouse situation
| Group | Quantity (only) | Death toll (only) | Ig dosage (ml/10g) | The result |
| Organize 1 group 2 groups 3 groups 4 | 5 5 5 5 | 4 1 0 0 | 0.1 0.05 0.025 0.012 | 1 survival, 4 dead 1 death, 4 all survivals of survival of survival |
1.2.4 according to D
mAnd D
nCarry out formal acute toxicity testing.
Formal experiment: 60 of NIH mices, male and female half and half.At D
nAnd D
mScope in be divided into 6 groups at random, 10 every group, male and female half and half.
1.2.4.1 with 2.5% Tween 80 aqueous solution preparating liquid, administration.
1.2.4.2 dosage: group 1 is a high dose, and dosage is the 0.15ml/10g group; Group 6 is a low dose group, and dosage is 0.036ml/10g.The dosage ratio is 1: 0.75 between group.
Table 8-3 death toll of formal experiment mice day
| Group | Date | ||||||
| The 1st day | The 2nd day | The 3rd day | The 4th day | The 5th day | The 6th day | The 7th day | |
| Organize 1 group of 2 groups of 3 groups of 4 groups of 5 groups of 6 ∑ | 5 4 3 2 2 0 16 | 2 1 1 1 1 0 6 | 1 2 0 0 0 0 3 | 0 0 0 0 0 0 0 | 0 0 0 0 0 0 0 | 0 0 0 0 0 0 0 | 0 0 0 0 0 0 0 |
Table 8-4 Oleum Citri Reticulatae chmice acute toxicity test dead mouse situation
Annotate: P is a mouse death rate, P
2Square value for mortality rate
2 results
The experiment situation: the activity of appearance successively reduces before the dead mouse, rolls up, reposes, and rapid breathing, shallow, fur does not have relaxation, does not see sialorrhea, rhinorrhea phenomenon.
Dead mouse situation record saw Table 8-3, did not promptly have dead the generation later at the 4th day.
Dead mice anatomy: be GI irritation, stomachus pyloricus hyperemia, gastrointestinal mucosa hyperemia, all the other organs are not seen obvious pathological change.Only the 4th group has 2 dead mices except that above situation is arranged, and it is redder to be also shown in pulmonary's color.
Calculate LD according to experimental result
50(seeing Table 8-4)
Calculate by improvement karber's method formula:
The mice median lethal dose(LD 50) LD of Pericarpium Citri Reticulatae extract
50=8.5612ml/kg; LogLD
50Standard error S
X50=0.0408;
Its LD
5095% fiducial limit: L
95=8.1747 ± 1.5715;
LD
5099% fiducial limit: L
99=8.1747 ± 2.0604
The toxic regression beeline equation of Pericarpium Citri Reticulatae extract chmice acute is: Y=4.2532x-1.0338
3 conclusions
The LD of Pericarpium Citri Reticulatae extract chmice acute toxicity test
50Be 8.5612ml/kg.
Dead mice is dissected discovery, and its toxicity performance is the same with oil of ginger, and mainly concentrating on stimulates gastrointestinal.Lung, heart, liver,spleen,kidney, reproductive system etc., outward appearance is not seen significant change.
The mice recovery situation: dead mouse generally all betides 1～2h after the administration, and dead mice subtracted greatly in the 2nd day.Organize 6 mices and promptly recovered in the 2nd day, movable normal, feed drinking-water is normal; By the 5th day, group 4, group 5 mices recovered normal; By the 6th day, all survival mice was all recovered fully.
In the experimentation, the 4th group has 2 dead mouse lung color exceptions, and other mices do not see this phenomenon, doubts to irritating the stomach operation unskilledly, has a little medicinal liquid to choke due to the pulmonary.
Embodiment 9: Rhizoma Zingiberis Recens extract gas chromatogram fingerprint map analyzing
1 material and method
1.1 sample: Rhizoma Zingiberis Recens extract (supercritical CO
2Extract).
1.2 instrument: Autosystem XL gas chromatograph, U.S. PERKIN-ELMER company.
1.3 experimental technique
1.3.1 chromatographic condition
Chromatographic column: PEG-20M, the quartz capillary column of 15m * 0.32mmID * 0.4 μ m thickness; Furnace temperature: 80 ℃, per minute raises 5 ℃ to 250 ℃ and keeps 26min; Injector temperature: 100 ℃, per minute raises 30 ℃ to 280 ℃ and keeps 54min; Carrier gas: high purity nitrogen; Post is pressed: 3.5PSI; FID:300 ℃; Split ratio: 20: 1; Hydrogen flow rate: 45ml/min; Air
Flow velocity: 450ml/min.
1.3.2 assay method
The accurate sample 0.2 μ l that draws, sample introduction is measured and the record collection of illustrative plates.
2 results
2.1 methodological study
In order to investigate the reliability of analytical method, the repeatability of its stability, instrument precision, experimental technique has been done corresponding investigation, the result proves that stability and repeatability are good.
2.1.1 stability test
Get same sample, detect every day in a week respectively, selects a peak in every group of peak, as Fig. 1, investigates the concordance of its retention time, the RSD of each chromatographic peak retention time≤0.58%.See Table 9-1.
The retention time of the selected chromatographic peak of table 9-1
| Chromatogram | Peak retention time (min) | ||||
| 1 | 2 | 3 | 4 | 5 | |
| ZING061 ZING068 ZING074 ZING079 ZING085 ZING091 | 2.087 2.061 2.084 2.089 2.094 2.092 | 9.930 9.887 9.933 9.967 9.994 9.960 | 22.615 22.625 22.645 22.650 22.643 22.642 | 29.288 29.101 29.109 29.122 29.097 29.090 | 43.555 43.579 43.292 43.614 43.586 43.529 |
| RSD(％) | 0.58 | 0.37 | 0.060 | 0.26 | 0.27 |
The retention time of the selected chromatographic peak of table 9-2
| Chromatogram | Peak retention time (min) | ||||
| 1 | 2 | 3 | 4 | 5 | |
| ZING092 ZING093 ZING094 ZING095 ZING096 ZING097 | 2.102 2.101 2.099 2.093 2.097 2.087 | 9.960 9.951 9.978 9.978 9.971 9.960 | 22.643 22.636 22.650 22.634 22.651 22.639 | 29.095 29.096 29.094 29.089 29.098 29.089 | 43.562 43.546 43.556 43.526 43.546 43.523 |
| RSD(％) | 0.27 | 0.11 | 0.032 | 0.013 | 0.036 |
2.1.2 instrument precision test
Get same duplicate samples, continuous sample introduction 6 times, the concordance of the retention time of investigation chromatographic peak, the RSD of each main chromatographic peak retention time≤0.27%.See Table 9-2.
2.1.3 repeatability test
Get 6 parts of the test samples of same lot number, detect, investigate the concordance of the retention time of chromatographic peak, the RSD of each main chromatographic peak retention time≤0.27% with method.See Table 9-3 and Fig. 2.
The retention time of the selected chromatographic peak of table 9-3
| Chromatogram | Peak retention time (min) | ||||
| 1 | 2 | 3 | 4 | 5 | |
| ZING086 ZING087 ZING088 ZING089 ZING090 ZING091 | 2.098 2.084 2.094 2.098 2.087 2.092 | 9.971 9.956 9.967 9.978 9.967 9.960 | 22.650 22.633 22.648 22.649 22.641 22.642 | 29.102 29.087 29.085 29.094 29.092 29.090 | 43.562 43.559 43.545 43.571 43.553 43.529 |
| RSD(％) | 0.27 | 0.078 | 0.029 | 0.023 | 0.034 |
2.2 the foundation of Rhizoma Zingiberis Recens extract GC fingerprint image
As Chinese medicine chromatographic fingerprint figure, it is simple, accurate that the assay method of its foundation is not only wanted, and will reflect the internal information of medicine as much as possible simultaneously in its chromatogram, so that can hold its feature on the whole, thus the reliability that assurance is identified.Because the different chromatogram that chromatographic column obtained may be different, the feature that reflects is also not exclusively the same, so this experiment selects the pillar of three kinds of opposed polarities: OV-1 for use, and OV-1701 and PEG-20M post have obtained 3 chromatograms, as Fig. 3.As can be seen from FIG., OV-1 and OV-1701 post can provide 109 and 105 chromatographic peaks, and the PEG-20M post can provide nearly 125 peaks, and chromatogram has a feature clearly, it is become by several peak groups, we can be divided into 5 groups to it artificially, retention time is first group from 1min～5min (80～105 ℃ of furnace temperature), 5min～15min (105～155 ℃ of furnace temperature) is second group, 15min～25min (155～205 ℃ of furnace temperature) is the 3rd group, 25min～40min (205～250 ℃ of furnace temperature) is the 4th group, and 40min～55min (250 ℃ of furnace temperature) is the 5th group.From the aspect of amount, its area of the content at second group of peak reaches 60%, has accounted for the overwhelming majority; And on the whole, each peak group is clear to debate, and separating degree is better, and these have all constituted the feature of Rhizoma Zingiberis Recens extract finger printing.
Chromatographic fingerprinting is used for TCD identification, all will enrich manyly with any single chemical reference substance as the information of differentiating that foundation provided.In the research of Rhizoma Zingiberis Recens volatile oil, often just a certain composition or certain constituents are measured [3-5], this obviously lacks specificity.Certainly, in research in recent years, also there are a lot of application GC/MS that it is carried out the analysis of qualitative, quantitative, but because the GC/MS price is comparatively expensive, and be unfavorable for applying.This research by repetition test relatively filters out the method for recording and narrating in the literary composition, standard experiment condition, the finger printing that makes acquisition separating degree, resolution, repeatability with stable aspect equal ideals comparatively.Can be used as the method for quality control of Rhizoma Zingiberis Recens medical material and preparation thereof.
3 conclusions
Originally studies show that and use method, condition described in GC chromatographic fingerprinting and the literary composition, is a kind of Rhizoma Zingiberis Recens extract to be differentiated simple, easy capable, accurate, controlled method with quality-monitoring.
Claims (3)
1, a kind of cardiovascular diseases's Rhizoma Zingiberis Recens, mixture of Pericarpium Citri Reticulatae extract for the treatment of is characterized in that its parts by weight are composed as follows:
Rhizoma Zingiberis Recens extract 100
Pericarpium Citri Reticulatae extract 0.1-200
Described Rhizoma Zingiberis Recens extract is the supercritical carbon dioxide extraction thing of Rhizoma Zingiberis Recens rhizome, and described Pericarpium Citri Reticulatae extract is the vapor distillation extract of the mature peel of Fructus Citri tangerinae and variety thereof.
2, treatment cardiovascular diseases's according to claim 1 Rhizoma Zingiberis Recens, the mixture of Pericarpium Citri Reticulatae extract is characterized in that its parts by weight are composed as follows:
Rhizoma Zingiberis Recens extract 100
Pericarpium Citri Reticulatae extract 50-100.
3, treatment cardiovascular diseases's according to claim 1 and 2 Rhizoma Zingiberis Recens, the mixture of Pericarpium Citri Reticulatae extract is characterized in that described Rhizoma Zingiberis Recens extract prepares by following method: exsiccant raw material Rhizoma Zingiberis Recens is dropped in the extraction kettle, fed supercritical CO
2Fluid at 15-30MPa, extracted 0.5-2 hour under 25-40 ℃; Extract enters in the knockout drum, at 8-15MPa, separates 0.5-2 hour under 40-60 ℃, obtains the Rhizoma Zingiberis Recens supercritical CO
2Extract; Described Pericarpium Citri Reticulatae extract is the vapor distillation extract of the mature peel of Fructus Citri tangerinae and variety thereof, prepares by following method: Pericarpium Citri Reticulatae is used vapor distillation 3-8 hour, collect distillate, get the Pericarpium Citri Reticulatae volatile oil extract after the oil-water separation.
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| CN102091315B (en) * | 2011-03-02 | 2012-05-23 | 青岛大学 | Ginger and dark plum fruit composition and preparation method thereof, and application of ginger and dark plum fruit composition in preparation of attenuation and synergy medicaments for radiotherapy and chemotherapy of cancers |
| CN102133384B (en) * | 2011-03-02 | 2012-03-14 | 青岛大学 | Ginger and dried orange peel composition, preparation method thereof and application of ginger and dried orange peel composition in preparation of attenuated synergistic medicaments in cancer radiotherapy and chemotherapy |
| CN102091312B (en) * | 2011-03-02 | 2012-05-23 | 青岛大学 | Ginger and wild pepper composition and preparation method thereof, and application of ginger and wild pepper composition in preparation of attenuation and synergy medicaments for radiotherapy and chemotherapy of cancers |
| CN102166335A (en) * | 2011-03-31 | 2011-08-31 | 青岛大学 | Ginger and long pepper composition as well as preparation method and application thereof in preparation of toxicity reducing and efficacy enhancing medicament in radiotherapy and chemotherapy of cancer |
| CN104982849B (en) * | 2015-06-09 | 2018-04-27 | 浙江茗皇天然食品开发股份有限公司 | Instant orange peel powder of aged odor type and preparation method thereof |
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| CN107375857A (en) * | 2016-05-16 | 2017-11-24 | 重庆百笑医疗设备有限公司 | A kind of moxibustion liver and gall system channels and network vessels regulation essential oils |
| CN109298089A (en) * | 2018-10-19 | 2019-02-01 | 南京中医药大学 | The method of quality control of rhizoma zingiberis |
| CN113952443A (en) * | 2021-12-06 | 2022-01-21 | 北京理工亘舒科技有限公司 | Traditional Chinese medicine composition with functions of dispelling cold dampness and reducing blood fat, preparation method and application thereof |
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| 姜对心脑血管***的药理作用 卢传坚，中药新药与临床药理，第14卷第5期 2003;浅述陈皮的药理及炮制研究 张群智，中国药业，第11卷第5期 2002 * |
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