CN1270963A - Longan polysaccharide extracting process - Google Patents
Longan polysaccharide extracting process Download PDFInfo
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- CN1270963A CN1270963A CN 00107538 CN00107538A CN1270963A CN 1270963 A CN1270963 A CN 1270963A CN 00107538 CN00107538 CN 00107538 CN 00107538 A CN00107538 A CN 00107538A CN 1270963 A CN1270963 A CN 1270963A
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Abstract
A longan polysaccharide extracting process includes such steps as defatting, enzymolysis and hydrolysis, water extraction, alcohol precipitation, drying, and crushing. The process can raise the activity of the extracted matter, maintain the effective component, color, fragrance and taste of longan, and raise longan polysaccharide extracting rate extremetly. The product is rich in protein, phosphorus, choline and trace elements and has the health functions of promoting intelligence growth, nourishing brain, resisting senility, invigorating heart, promoting blood circulation, etc.
Description
The invention relates to the extraction process of a kind of longan, more particularly about a kind of longan polysaccharide extracting process.
Longan aril Arillus Langan is the arillus of sapindaceous plant longan DimocanpusLangan Lour.Longan is one of four your name fruit of China people high praise, and Chinese materia medica claims that longan aril is garden, an osmanthus meat.Longan flavor sweet flat, nontoxic, go into spleen, the heart channel of Hang-Shaoyin.Ming Dynasty pharmacy man LI Shi-Zhen has been given very high evaluation to the nutritive value of longan with to the effect of human health, and he says in Compendium of Material Medica: food is expensive with lichee, and intelligence development then longan is good.LI Shi-Zhen is also pointed out: it is clever that longan is obeyed strong soul for a long time, and it is not old to make light of one's life by commiting suicide.The traditional Chinese medical science thinks that longan has the effect of " being better than genseng ".Modern science is discovered longan except that containing a large amount of glucose, fructose and sucrose, also contains abundant polysaccharide, but is not much accounted of and big quantity research always.
The value of the mensuration of relevant longan chemical ingredients, the characteristics of pharmacological action, health nutrient had some reports in recent years, but did not then appear in the newspapers as yet for the extracting method and the technology of longan polysaccharide.
The purpose of this invention is to provide a kind of longan polysaccharide that scientifically extracts, and can keep the activity of longan polysaccharide and the local flavor of former longan, improve the technology of the extraction yield of longan polysaccharide to greatest extent.
These purposes of the present invention and other purposes will further be set forth and illustrate by following detailed description.
Longan polysaccharide extracting process of the present invention comprises steps such as degreasing, double-enzyme hydrolysis, flooding, alcohol precipitation and drying and crushing.
Further, longan polysaccharide extracting process of the present invention comprises following concrete steps:
A, defatting step: will shell longan or dried longan are pulverized, and add 1.5～3.0 liter alcohol by 1 kilogram of shell longan or dried longan, 50～80 ℃ of backflow lixiviates 0.6～1.5 hour, or soak at room temperature 3～6 hours, solid collected by filtration thing, water flushing 1～2 time.
B, double-enzyme hydrolysis step: above-mentioned solids is added water 1.5～3.0 liters, use acid to transfer pH value to 1.8～3.0, press the per kilogram raw material and add 4～15 gram stomach en-s, in 30～40 ℃ of insulations, stirring 3～5 hours; Adding alkali accent pH value then is 7～9, presses the per kilogram raw material and adds 4～15 gram trypsinase, in 30～40 ℃ of insulations, stirring 3～5 hours, adds acid accent pH value then for acid.
C, flooding step: above-mentioned raw materials is added 1～5 liter water again, put pressure kettle, or in 100 ℃ of lixiviates 2～4 hours, filter and remove slag and insolubles, the filtrate neutralization in 105～115 ℃ of lixiviates 0.5～1.5 hour.
D, alcohol precipitation step: above-mentioned vat liquor is concentrated into half of former cumulative volume at 30～70 ℃, and adding medical alcohol is 50～70% to ethanol concn, and placement is spent the night, and leaches throw out, centrifuge dripping, the longan polysaccharide solids that must wet.
E, drying and crushing step: with above-mentioned wet solids, evenly spread and place drip pan,, dried 2～8 hours at 60～80 ℃, pulverize light grey longan polysaccharide finished product; Or adopt with above-mentioned wet solids centrifuge dripping, wash, drying under reduced pressure, drying pressure is 20～200Pa, the time is 5～8 hours, pulverize light grey longan polysaccharide finished product.
In the present invention, the water that is adopted is deionized water, distilled water, pure water and contains one of mineral ion water.
The longan polysaccharide that the present invention extracts is light gray-light salmon, and nothing is smelt, and water soluble is a kind of dextran that is similar to lentinan, tentatively is defined as beta-glucan, finds out to have typical dextran feature from infrared spectra.
The present invention adopts Composite Double zymotechnic and dry extraction novel process can greatly improve the activity and the immunity of longan extract, keeps longan effective constituent and original color, smell and taste, has improved the extraction yield of longan polysaccharide to greatest extent.
The product of preparing with method of the present invention has very high biological activity and immunity, and rich in proteins, phosphorus, choline, trace element, is present known intelligence development, brain tonic, anti-ageing, bushing is promoted blood circulation, relieving restlessness is dispelled dry, eyeshield eyesight-improving health care function treasure.
In the present invention, all raw material, half products all can be buied from market.
As non-refering in particular to, all parts, amount are the weight unit of gross weight for the basis in the present invention.
Below further specify the present invention by specific embodiment, but embodiment only be used for the explanation can not limit the scope of the invention.
Example 1: step is extracted longan polysaccharide by the following method.
A, defatting step: will shell longan or dried longan are pulverized, and add 2.5 liter alcohol by 1 kilogram of shell longan or dried longan, and 65 ℃ of backflow lixiviates 0.8 hour, the solid collected by filtration thing was with distilled water drip washing 1～2 time;
B, double-enzyme hydrolysis step: with above-mentioned solids adding distil water 2 liters, transfer pH value to 2, press the per kilogram raw material and add 8 gram stomach en-s, in 35～40 ℃ of insulations, stirring 4 hours with hydrochloric acid; Hydro-oxidation sodium accent pH value is 9 then, presses the per kilogram raw material and adds 8 gram trypsinase, and in 35～40 ℃ of insulations, stirring 4 hours, adding hydrochloric acid accent pH value then was 4;
C, flooding step: above-mentioned raw materials is added 3 liter distilled water again, put pressure kettle, filter and remove slag and insolubles, the filtrate neutralization in 110 ℃ of lixiviates 1 hour;
D, alcohol precipitation step: above-mentioned vat liquor is concentrated into half of former cumulative volume at 70 ℃, and adding medical alcohol is 60～70% to ethanol concn, and placement is spent the night, and leaches throw out, centrifuge dripping, the longan polysaccharide solids that must wet;
E, drying and crushing step: with above-mentioned wet solids, evenly spread and place drip pan,, dried 6 hours at 60～80 ℃, pulverize light grey longan polysaccharide finished product, yield be per kilogram longan (dry product) 50～80 the gram finished products.
Example 2: step is extracted longan polysaccharide by the following method.
A, defatting step: will shell longan or dried longan are pulverized, and add 2.0 liter alcohol by 1 kilogram of shell longan or dried longan, and 50 ℃ of backflow lixiviates 1.5 hours, the solid collected by filtration thing was with deionized water rinsing 1～2 time;
B, double-enzyme hydrolysis step: above-mentioned solids is added deionized water 1.9 liters, transfer pH value to 2.5, press the per kilogram raw material and add 10 gram stomach en-s, in 30～40 ℃ of insulations, stirring 4 hours with hydrochloric acid; Hydro-oxidation sodium accent pH value is 8 then, presses the per kilogram raw material and adds 10 gram trypsinase, and in 30～40 ℃ of insulations, stirring 4 hours, it was acid adding hydrochloric acid accent pH value then;
C, flooding step: above-mentioned raw materials is added 3 liter deionized waters again, put pressure kettle, filter and remove slag and insolubles, the filtrate neutralization in 110 ℃ of lixiviates 1.0 hours;
D, alcohol precipitation step: above-mentioned vat liquor is concentrated into half of former cumulative volume at 70 ℃, and adding medical alcohol is 60～70% to ethanol concn, and placement is spent the night, and leaches throw out, centrifuge dripping, the longan polysaccharide solids that must wet;
E, drying and crushing step: with above-mentioned wet solids, evenly spread and place drip pan,, dried 2～8 hours at 60～80 ℃, pulverize light grey longan polysaccharide finished product, yield be per kilogram longan (dry product) 50～80 the gram finished products.
Example 3: step is extracted longan polysaccharide by the following method.
A, defatting step: will shell longan or dried longan are pulverized, and add 2.0 liter alcohol by 1 kilogram of shell longan or dried longan, soak at room temperature 5 hours, and the solid collected by filtration thing is with purified rinse water 1～2 time;
B, double-enzyme hydrolysis step: above-mentioned solids is added pure water 4.0 liters, transfer pH value to 2.0, press the per kilogram raw material and add 6 gram stomach en-s, in 30 ℃ of insulations, stirring 3 hours with hydrochloric acid; Hydro-oxidation sodium accent pH value is 9 then, presses the per kilogram raw material and adds 6 gram trypsinase, and in 30 ℃ of insulations, stirring 3 hours, adding hydrochloric acid accent pH value then was 4;
C, flooding step: above-mentioned raw materials is added 4 liter pure water again, in 100 ℃ of lixiviates 2～4 hours, filter and remove slag and insolubles, the filtrate neutralization;
D, alcohol precipitation step: above-mentioned vat liquor is concentrated into half of former cumulative volume at 40 ℃, and adding medical alcohol is 70% to ethanol concn, and placement is spent the night, and leaches throw out, centrifuge dripping, the longan polysaccharide solids that must wet;
E, drying and crushing step: with above-mentioned wet solids centrifuge dripping, the washing, drying under reduced pressure, drying pressure is 100Pa, the time is 6 hours, pulverize light grey longan polysaccharide finished product, yield be per kilogram longan (dry product) 70～90 the gram finished products.
Claims (8)
1, a kind of longan polysaccharide extracting process is characterized in that comprising steps such as degreasing, double-enzyme hydrolysis, flooding, alcohol precipitation and drying and crushing.
2, longan polysaccharide extracting process as claimed in claim 1, it is characterized in that described defatting step comprises longan or the dried longan pulverizing of shelling, by 1 kilogram of shell longan or dried longan adding 1.5～3.0 liter alcohol, 50～80 ℃ of backflow lixiviates 0.6～1.5 hour, or soak at room temperature 3～6 hours, the solid collected by filtration thing, water flushing 1～2 time.
3, longan polysaccharide extracting process as claimed in claim 1, it is characterized in that described double-enzyme hydrolysis step comprises adds water 1.5～3.0 liters with above-mentioned solids, transfer pH value to 1.8～3.0 with acid, press the per kilogram raw material and add 4～15 gram stomach en-s, in 30～40 ℃ of insulations, stirring 3～5 hours; Adding alkali accent pH value then is 7～9, presses the per kilogram raw material and adds 4～15 gram trypsinase, in 30～40 ℃ of insulations, stirring 3～5 hours, adds acid accent pH value then for acid.
4, longan polysaccharide extracting process as claimed in claim 1, it is characterized in that described flooding step comprises adds 1～5 liter water again with above-mentioned raw materials, put pressure kettle in 105～115 ℃ of lixiviates 0.5～1.5 hour, or in 100 ℃ of lixiviates 2～4 hours, filter and remove slag and insolubles, the filtrate neutralization.
5, longan polysaccharide extracting process as claimed in claim 1, it is characterized in that described alcohol precipitation step comprises is concentrated into half of former cumulative volume with above-mentioned vat liquor at 30～70 ℃, adding medical alcohol to ethanol concn is 50～70%, placement is spent the night, leach throw out, centrifuge dripping, the longan polysaccharide solids that must wet.
6, longan polysaccharide extracting process as claimed in claim 1 is characterized in that described drying and crushing step comprises above-mentioned wet solids, evenly spreads and places drip pan, at 60～80 ℃, dries 2～8 hours, pulverize light grey longan polysaccharide finished product.
7, longan polysaccharide extracting process as claimed in claim 1 is characterized in that described drying and crushing step comprises above-mentioned wet solids centrifuge dripping, washing, drying under reduced pressure, drying pressure is 20～200Pa, and the time is 5～8 hours, pulverize light grey longan polysaccharide finished product.
8,, it is characterized in that described water is deionized water, distilled water, pure water and contains mineral ion water as the described longan polysaccharide extracting process of claim 1～7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00107538A CN1100792C (en) | 2000-05-18 | 2000-05-18 | Longan polysaccharide extracting process |
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| CN00107538A CN1100792C (en) | 2000-05-18 | 2000-05-18 | Longan polysaccharide extracting process |
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| CN1270963A true CN1270963A (en) | 2000-10-25 |
| CN1100792C CN1100792C (en) | 2003-02-05 |
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| CN00107538A Expired - Fee Related CN1100792C (en) | 2000-05-18 | 2000-05-18 | Longan polysaccharide extracting process |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1305908C (en) * | 2002-11-29 | 2007-03-21 | 湖北大学 | Extracting technology for mountain ophiopogon root polysaccharide |
| CN100429236C (en) * | 2006-11-03 | 2008-10-29 | 浙江大学 | Method of extracting soluble polysaccharide in bitter vegetable |
| CN102050882A (en) * | 2010-12-24 | 2011-05-11 | 广东省农业科学院农业生物技术研究所 | Longan proteoglycan as well as preparation method and application thereof |
| CN102070724A (en) * | 2010-12-24 | 2011-05-25 | 广东省农业科学院农业生物技术研究所 | Method for separating neutral longan polysaccharide fraction |
| CN101433620B (en) * | 2008-12-10 | 2011-09-28 | 广东省农业科学院农业生物技术研究所 | Longan water-soluble active substance as well as extraction method and use thereof |
| CN102234671A (en) * | 2010-05-07 | 2011-11-09 | 中国农业科学院农产品加工研究所 | Method for extracting polysaccharide from longan pulp |
| CN106167531A (en) * | 2016-08-16 | 2016-11-30 | 集美大学 | A kind of method of enzyme process assisted extraction Arillus Longan polysaccharide |
| CN109497119A (en) * | 2018-11-23 | 2019-03-22 | 翁航 | A kind of longan cake and preparation method |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0086233A1 (en) * | 1981-04-30 | 1983-08-24 | Zenyaku Kogyo Kabushiki Kaisha | Polysaccharide ps-a isolated from the plant of genus epimedium violaceum morr. et decne., process for its preparation, and infection-preventing agent and immunostimulating agent containing the polysaccharide as effective ingredient |
| JPH01261403A (en) * | 1988-04-12 | 1989-10-18 | Nippon Flour Mills Co Ltd | New substance and anticancer drug |
| CN1061976A (en) * | 1991-10-31 | 1992-06-17 | 张孟铸 | Utilize the fresh mushroom handle to extract the method for lentinan |
| CN1069650C (en) * | 1995-12-29 | 2001-08-15 | 中国科学院上海药物研究所 | Method for separation and purification of lentinan |
-
2000
- 2000-05-18 CN CN00107538A patent/CN1100792C/en not_active Expired - Fee Related
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1305908C (en) * | 2002-11-29 | 2007-03-21 | 湖北大学 | Extracting technology for mountain ophiopogon root polysaccharide |
| CN100429236C (en) * | 2006-11-03 | 2008-10-29 | 浙江大学 | Method of extracting soluble polysaccharide in bitter vegetable |
| CN101433620B (en) * | 2008-12-10 | 2011-09-28 | 广东省农业科学院农业生物技术研究所 | Longan water-soluble active substance as well as extraction method and use thereof |
| CN102234671A (en) * | 2010-05-07 | 2011-11-09 | 中国农业科学院农产品加工研究所 | Method for extracting polysaccharide from longan pulp |
| CN102234671B (en) * | 2010-05-07 | 2013-11-06 | 中国农业科学院农产品加工研究所 | Method for extracting polysaccharide from longan pulp |
| CN102050882A (en) * | 2010-12-24 | 2011-05-11 | 广东省农业科学院农业生物技术研究所 | Longan proteoglycan as well as preparation method and application thereof |
| CN102070724A (en) * | 2010-12-24 | 2011-05-25 | 广东省农业科学院农业生物技术研究所 | Method for separating neutral longan polysaccharide fraction |
| CN102070724B (en) * | 2010-12-24 | 2012-04-18 | 广东省农业科学院农业生物技术研究所 | Method for separating neutral longan polysaccharide fraction |
| CN102050882B (en) * | 2010-12-24 | 2012-05-23 | 广东省农业科学院农业生物技术研究所 | Longan proteoglycan as well as preparation method and application thereof |
| CN106167531A (en) * | 2016-08-16 | 2016-11-30 | 集美大学 | A kind of method of enzyme process assisted extraction Arillus Longan polysaccharide |
| CN106167531B (en) * | 2016-08-16 | 2018-09-21 | 集美大学 | A kind of method of enzyme process assisted extraction longan polysaccharide |
| CN109497119A (en) * | 2018-11-23 | 2019-03-22 | 翁航 | A kind of longan cake and preparation method |
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| Publication number | Publication date |
|---|---|
| CN1100792C (en) | 2003-02-05 |
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