Lentinan, trade(brand)name power are carried energy, English name Lentinan; Be a kind of immunity regulating type antitumour auxiliary drug.
The lentinan system polysaccharide that the extraction separation purifying obtains from the umbrella mushroom section fungi mushroom fruiting body of artificial culture.The various countries scientist just carries out extensive studies to all kinds of polysaccharide as far back as early seventies, the discovery polysaccharide not only can be treated the cancer that body immune system is subjected to major injury, can treat the damaged disease of panimmunity again, as chronic viral hepatitis and some drug-resistant bacteria or the viral chronic disease that causes, can also treat the autoimmune disease such as rheumatosis.The sugar chain of discovered in recent years polysaccharide has conclusive effect in molecular biology, it can control cell fission and differentiation, regulates the growth and the aging of cell, and in addition, polysaccharide pharmaceutically is being a kind of good adjuvant again.
Because polysaccharide is the polarity macromolecular cpd, can cause the polysaccharid glucoside chain break under the acidic conditions.Its extraction separation is very complicated, adopts boiling water mostly, and alkaline solution extracts.Free protein in the extracting solution is mainly removed with three kinds of methods such as Sevage method, Freon 113 method and Tricholroacetic Acid method, the first two kind method is applied in the microbial polysaccharide more, the latter is applied in the vegetable polysaccharides more, conjugated protein remove available proteolytic enzyme, small molecular weight impurity is removed available dialysis method, the continuous dialysis apparatus dialysis of external employing, also available ultra-filtration method obtains the polysaccharide of certain molecular weight scope.
The purification process of polysaccharide is a lot, and branch's precipitator method, salting-out process, metal complex method, the quaternary amine precipitator method are arranged.Diethylamino ethyl-Mierocrystalline cellulose method (DEAE-Mierocrystalline cellulose method) and other various dissimilar gel filtration chromatography methods.Measure polysaccharide with colorimetric methods such as sulfuric acid-phynol methods.
The one-component (I) that lentinan system obtains from mushroom fruiting body
Its extraction separation is very complicated and yield is very low, the cost height.The clear 48-6767 of Japanese Patent (1973); Clear 49-484 (1974).Recently once had report to adopt the gel filtration chromatography method, but this method can only obtain use sample for research on a small quantity, can't be applied to produce, and since gel cost an arm and a leg and make cost very high, owing to prior art remains in many shortcomings.The object of the invention is produced the separation purification method of the lentinan that cost is low in order to seek a kind of new comparatively easy being easy to.
The present invention implements through the following steps:
Concentrate → the enriched material lyophilize.
Get the raw material mushroom, use hot water extraction, extracting solution is evaporated to small volume, stirs slowly to add ethanol down, make it produce precipitation, precipitation is placed in the dialysis tubing and dialyses through water-soluble Jie, and dialyzate concentrates, ethanol sedimentation, after washing with alcohol, vacuum-drying gets raw product to precipitation again.The thick lentinan of gained is soluble in water, after the DEAE-cellulose adsorption, washes with water, uses the dilute alkaline soln wash-out, and used alkali can be Na
2CO
3, K
2CO
3, NaHCO
3, KHCO
3, NaOH or KOH, the concentration of alkali elutriant can be 1~2mol/L, the elutriant fraction collection is got sugared reacting positive partly, with the hydrochloric acid neutralization, places on the ultra-fine filter and filters, and does not get to see through liquid and concentrate, lyophilize promptly gets the single component of lentinan.
Embodiment 1
Get 10 kilograms of qualified raw material dried thin mushrooms, after the pulverizing, place the reactor of strap clamp cover, add clear water, the add-on of water is about 1.5 times of raw material volume, jacket steam is heated to boiling, kept 6 hours under boiling then, filter, residue extracts three times with aforesaid method again, merge whole filtrates, be evaporated to small volume, stir the ethanol that slowly adds isopyknic 95% above concentration down, leave standstill and placed 4 hours, precipitation produces, the supernatant liquor that inclines, lower sediment is through the centrifugal precipitation that obtains, and precipitation is behind ethanol Xian Di, soluble in water, place dialysis tubing to tap water dialysis 3 days, dialyzate is evaporated to small volume, and the concentration that adds equal-volume under stirring is the ethanol more than 95%, after static 4 hours, centrifugal must the precipitation, precipitation are through ethanol, and ether is in succession behind the Xian Di, dry in 60 ℃ vacuum drier, get about 100 grams of lentinan crude product.
Crude product 100g is soluble in water, and is injected in the 1500g DEAE-Mierocrystalline cellulose at first water Xian Di, uses NaOH 1mole/L eluant solution then, collects the elutriant HCl neutralization of the sugared reacting positive part of NaOH wash-out.Ultra-filtration on amicon, the fenestra of ultra-filtration is got not permeation parts through being 100,000, is concentrated into small volume, and lyophilize gets single component lentinan 2g, and total recovery is 0.02%, lentinan content 97.3%.
Embodiment 2:
Get qualified raw material dried thin mushroom 12Kg, after the pulverizing, place the reactor of strap clamp cover, add clear water, the add-on of water is about 1.8 times of raw material volume, jacket steam is heated to boiling, under boiling, kept 7 hours then, filter, residue is pressed said process again and is extracted 2 times, merges all filtrates, be evaporated to small volume, stirring the concentration that adds equal-volume down is 95% above ethanol, and supernatant liquor is toppled in static placement 5 hours, lower sediment is through the centrifugal precipitation that obtains, precipitation is after washing with alcohol, and is soluble in water, places dialysis tubing to water dialysis 3 days, dialyzate is evaporated to small volume, stir the ethanol that adds equal-volume down, static 4 hours, topple over supernatant liquor, lower sediment is through centrifugal, obtain precipitation, precipitation is again through ethanol, and ether washs in succession, get the about 126g of lentinan crude product 60 ℃ of following vacuum-dryings, crude product 126g is soluble in water, is added to (1500g) in the DEAE-Mierocrystalline cellulose, and waste liquid discards, the DEAE-Mierocrystalline cellulose that contains lentinan washes with water earlier, collect the alkali elutriant and do sugared reaction test with the dilute NaOH solution wash-out then, collect the part of sugared reacting positive, sugared reacting positive portion is done ultra-filtration.The resistance filter value molecular weight of filtering membrane is 100,000, gets and is not excluded part, is concentrated to the lentinan 2.64g that the small volume lyophilize gets single component, total recovery is 0.022%, product is through Congo red reaction, and ultraviolet determination is found to be maximum absorption band at 480~495nm place then.Survey ultraviolet after the neutralization again, its maximum absorption is moved to 505-520nm, and two scopes of this reaction uv-absorbing are peculiar by β (1 → 3) dextran.Lentinan belongs to β (1 → 3) dextran, so two obtained the maximum absorption also should be arranged.
Infrared spectra has 3500~3300cm
-1(the O-H stretching vibration that the sugar ring is gone up OH); 2920~2800cm
-1(the sugar ring is gone up the vibration of C-H angle); 1100~1000cm
-1(the sugar ring is gone up the C-O stretching vibration); 890cm
-1(the anomeric carbon characteristic absorbance of beta comfiguration) waits four charateristic avsorption bands,
13C NMR (Nuclear Magnetic Resonance) spectrum tool 104.66ppm (the C-1 signal of β-1,3 dextran); (88.04ppm C-3 signal); (77.92ppm C-5 signal); (75.19ppm C-2 signal); (70.13ppm C-4 signal) and; (62.17ppm C-6 signal).
Assay:
Lentinan peak and the impurity peaks that obtains with high performance liquid chromatography, be converted into concentration, can try to achieve the content of lentinan, or adopt anthrone colorimetry (contrast is the standard lentinan) to measure content by peak area.