CN1262439A - Contraceptive test paper for safe period and its test method - Google Patents

Abstract

A contraceptive test paper for safe period and its test method are disclosed. The specific LH-resistant monoclonal antibody and another LH-resistant golen mark probe are respectively fixed to some linked regional band on cellulose nitrate membrane. Said membrane is immersed in the sampled urine and a specific combination takes place between relative LH antigen and LH-resistant golden mark antibody, which can be recognized by LH-resistant monoclonal antibody while the golden mark probe stayed in the region shows relative red, so determining the LH peak in one month and in turn the ovulation period correctly.

Description

A kind of safe period test paper and detection method thereof

The present invention relates to a kind of contraception of woman articles for use and detection method thereof, promptly a kind of safe period test paper and detection method thereof.

Ovulation is the critical event that betides in the ovarian cycle.Usually, once ovulation only took place in every month in normal women of child-bearing age.If can measure this onset of ovulation exactly, avoid just reaching the purpose of contraception effectively in onset of ovulation side together.The result of reproductive endocrine research shows that LH (lutropin) can promote the discharging of mature egg and the formation of corpus luteum.Thereby before ovulation, the LH value in blood and the urine raises, and reaches peak value.Most of women (95%) after LH reaches peak value soon (behind the LH peak 9.5-23 hour, average 16.5 hours) normal ovulation promptly takes place, thereby LH can be used as the accurate sign that advance notice is ovulated to time of peaking.Therefore, correctly judge ovulation period, extremely important for instructing safe period.Simultaneously, the monitoring of ovulation also is the important means of sterility treatment, indispensable composition link in the medical skills such as still artificial insemination, test-tube baby, for those fallopian tubals lack as: stop up or salpingoplasty loser or the uncomfortable patients with infertility that is fit to do anastomosis of tube, in vitro fertilization is a kind of therapy measure, and can the crux of the success or failure of in-vitro fertilization (IVF) then be obtain ripe egg mother cell in time in the spontaneous ovulation first few minutes.Therefore, forecast with unerring accuracy ovulation period for determining that the best artificial insemination moment and the success or failure of in vitro being fertilized are most important.Ovulation prediction also has profound practical value far away at precious mammal and breeding stock aspect such as cultivate, develop animal husbandry except the future that is widely used in mankind fertility, sterile and birth control, THE REPRODUCTION OF THE GIANT PANDA is exactly a lively example.This shows that the ovulation prediction technology has important practice significance.Existing ovulation prediction method has women's oneself's detection method and clinical detection method etc. following several: 1, women oneself detection method: this type of ovulation prediction method mostly is the couple at child-bearing age greatly and grasps voluntarily, they need not the instrument and equipment by means of precision, more simple and easy to do, thereby used till today by people, specifically comprise: (1) predication method onset of ovulation: it can only predict possible ovulation date and scope, can't definitely predict the accurate date, thereby it can only reach fertilization in vitro and can not be used for artificial insemination as a kind of reference of safe period.Moreover human ovulation often is subjected to the restriction of factors such as physiology and psychology, and under given conditions, also may not be a relative constant onset of ovulation, thus this method easily error to take place and cause failure, its mortality be the 14.4-47.0/100 women years.(2) examination of basal body temperature method: this method is with the formation of basal body temperature as the detection corpus luteum, measure the onset of ovulation to reach, because it can only show indirectly that ovulation has taken place or corpus luteum forms, but it can't predict the generation of ovulation, so this method can only may be ovulated in retrospective indication, and can not predict moment of ovulation perspectively, according to Marshall, its mortality is 6.6/100 women years.(3) cervical mucus observation: it with " dry epoch " that and then occur behind menstrual onset as pregnancy period not, dry epoch after 4 days about, gluing sense is arranged and inextensibility mucus occurs as G type mucus, the preovulatory phase, it is rare full thin that cervical mucus begins to become, transparent, limpid and lubricious as E type mucus, be called the last day of appearance E type mucus " rush day ", i.e. ovulation is about to take place, after rush day the 3rd day, be can the conceptive date, cervical mucus becomes thickness again again after the ovulation, opaque, secretory volume also reduces, begin to next menstrual onset about totally 11 days from this moment, this phase is pregnancy period not, and this method also is applicable to puerperium, lactation, puberty and climacteric; Billings instructs contraception with this method, and pregnancy rate is the 0.5-2.9/100 women years, thus it than the onset of ovulation predication method and examination of basal body temperature method slightly excellent, yet it can't calculate to a nicety the ovulation date, reach in vitro and be fertilized so can not be used for artificial insemination.2, modern clinical detection method: the mensuration of (1) luteinizing principle (LH) peak value: arrive the sign of the time of peaking with LH as the advance notice ovulation.The movable definite time was difficult to estimate before the irregularities that anterior pituitary LH discharges made ovulation, owing to need frequently get blood specimen, made the accurate mensuration of LH peak value that certain difficulty be arranged.(2) mensuration of progesterone: the obvious rising according to ovulation back blood progesterone concentration comes predicting ovulation, and this method can obtain the result in back 3 hours in blood sampling, and can repeat repeatedly every day.(3) blood estradiol is measured: come predicting ovulation with estradiol value in the mensuration blood, because the blood estradiol value can only reflect the growth and the maturity of folliculus, be difficult to independent sign as predicting ovulation.(4) mensuration of N-acetyl-(NAG) in the saliva: to measure in the saliva NAG vigor as a kind of means of ovulation prediction.(5) other methods of clinical observation: adopt and observe the uterine neck mouth, analyze the wire drawing degree of cervical mucus and the formation of fernlike crystal, check the cylinder PI of vaginal cell, the supplementary means of the methods such as size of ovarian follicle as predicting ovulation checked in the ultrasound wave video picture.In addition to the above methods, developed new ovulation prediction methods such as reproductive hormone glucosiduronate, electronic temperature measurement device, electrolytes determined in the urine in recent years again, but be not used for clinical at present as yet on a large scale.In the above-mentioned the whole bag of tricks, though that the method that every women can often grasp after the doctor instructs voluntarily is simple and easy to is feasible, accuracy is not high, the failure of instructing contraception to cause sometimes with it.All genus clinical observations and measuring method (wherein particularly the mensuration of blood LH rising initial value and blood progesterone value), though accuracy height, but method is easy inadequately, and need certain instrument and equipment, in general only be used for artificial insemination and fertilization in vitro, inconvenience is used for family and instructs safe period.Utilize the immunochromatography single stage method of gold mark probe development to be widely used in various clinical diagnosises in recent years, the immunochromatography single stage method is a certain district band that special antibody is fixed in nitrocellulose filter earlier, after this dry nitrocellulose filter one end immerses sample (urine or serum), because capillary action, sample will move forward along this film, when moving to when being fixed with antibody regional, to be discerned by this antibody with the corresponding antigen of golden labeling antibody generation specific bond in the sample with different binding sites, thus the specific bond of generation sandwich style.Like this, be stranded in this regional collaurum and will show corresponding redness, can detect the antigen of different content, reach specific immunodiagnosis.At present, the detection medicine box of existing panimmunity collaurum, its finished product have paper disk method, bag by various ways such as globule or film methods, and the wherein the most frequently used early pregnancy that has detects test paper, ovulation tests test paper etc.These test paper are easy and simple to handle fast, only need an end of test strips is immersed in the Women's Urine, take out traverse after 1 minute, can judge whether to become pregnant or not ovulation according to the change in color degree after 1-10 minute, thereby be subjected to very much practitioner and the user's of family welcome, the production and selling of this class box is all arranged as many countries such as the U.S., Britain, Canada and Japan.Also there are many companies in China at present at production and selling gold mark early pregnancy test paper, these test paper can detect by WHO standard that the HCG minimum flow is 25mIU/ml in the urine, can in conceived 7-10 days, detect early, the cell strain of monoclonal antibody of its employed anti-lutropin (LH) is provided by U.S. co-partnership company, simultaneously used paper shape material is also available from the U.S., thereby cost is higher, and price is more expensive.In addition, it all is with reference to foreign standard that these two kinds of LH detect test paper, is foundation level with urine LH＜20mIU/ml promptly, is about to occur for LH peak in this menstrual cycle urine more than or equal to 40mIU/ml.And detect the result of nearly 300 routine normal women of child-bearing age urina sanguinis with radioimmunoassay according to some domestic research datas and applicant, a lot of women's urine LH peak value is lower than 40mIU/ml, like this, above-mentioned two kinds of test paper just can't detect this class women's LH peak value exactly, cause false negative, omission occurs.

The present invention is directed to above-mentioned existing problems, its purpose is to provide a kind of cheap, the safe period test paper and detection method thereof that can accurately measure the LH peak value.

The present invention utilizes immunochromatography single stage method principle, special anti-LH monoclonal antibody is fixed in a certain district band of nitrocellulose filter earlier, after this dry cellulose nitrate one end immerses urine, because capillarity, sample will move forward along this film, when moving to when being fixed with antibody regional, with the corresponding LH antigen of another anti-LH gold labeling antibody generation specific bond the anti-LH antibody on the tunicle is discerned in the sample, the specific bond of sandwich style takes place, meanwhile, being stranded in this regional collaurum will show corresponding red.Like this, can measure women of child-bearing age's LH peak value per month by measuring the content of the lutropin (LH) in the urine, thereby determine its corresponding onset of ovulation.It is measured the ria-determination kit of LH and enzyme with traditional being used to and exempts to measure kit and compare, LH detects test paper and has simple to operate quick, the result is easy to the characteristics of judgement, it only needs an end of test strips is immersed in women's urine sample, take out traverse after 1 minute, can disease judge whether ovulation according to the change in color degree after 5 minutes.

The present invention has accurate, quick, convenient, the with low cost characteristics of test, it can be widely used in family, hospital and other place, because it can accurately measure the ovulation period in each midcycle of normal women of child-bearing age, thereby scientifically instruct " safe period " contraception, the fundamental state policy that can be family planning provides a kind of up-to-date product, and this product also can be used for the detection of artificial insemination, test-tube baby and infertility.

Fig. 1 is the main TV structure figure of LH test strips of the present invention.

Fig. 2 is that LH test strips of the present invention is shown as positive findings figure when being applied to test.

Fig. 3 is that LH test strips of the present invention is shown as negative findings figure when being applied to test.

Fig. 4 is the sectional structure chart of overlooking of Fig. 1 of the present invention.

Below in conjunction with drawings and Examples the present invention is described in detail.

The present invention utilizes immunochromatography single stage method principle, special anti-LH monoclonal antibody and another anti-LH gold are marked a certain contiguous area band that probe is individually fixed in nitrocellulose filter, utilization from menstruation immerse every day later the urine sample of this film corresponding LH antigen can with anti-LH gold mark probe antibody generation specific bond after and by anti-LH monoclonal antibody identification, the specific bond of sandwich style promptly takes place, thereby by measuring the content of the lutropin LH in the urine, can measure women of child-bearing age's LH peak value per month, accurately to determine its corresponding onset of ovulation, the gold mark probe that promptly is stranded in this zone will show corresponding red, when the red display line occurs, just indication enters the onset of ovulation, when continuous 2 days of the same day was measured and not have colour band always and manifest during from the red display heading line off, before to next menstruation was absolute " safe period " during this period of time.On base plate (7), be fixed with the fine plain rete (5) of the nitric acid of anti-LH monoclonal antibody, be fixed with one deck absorbent paper layer (6) and glass fiber paper layer (1) at the two ends of this rete (5) respectively, and on folder between glass fiber paper layer (1) and the nitrocellulose filter (5), have the plain rete (2) of glass fibre of anti-LH Au probe.Bring into use later the test paper inspection from menstruation, use one every day, measured the red display line always and occurred, just indication enters the onset of ovulation; Measure again when display line is red to disappear, and measured when not having colour band always and manifesting from continuous 2 days of this day, can stop using, and before at this moment come to next menstruation be absolute " safe period " during this period of time, promptly when recording LH＜25mIU/ml, all do not have colour band and manifest, equal color band manifests during LH＞25mIU/ml, and the concentration of LH is high more, and the colour band on the test strips is also just dark more.Cellulose nitrate rete (5) is provided with p-wire (3) and control line (4).The present invention has done following test:

One, the mensuration of sensitivity: contain LH standard items (Sigma company product) 0,5,10,20,25,50,100,200mIU/ml with this detection paper, draw lowest detection and be limited to 25mIU/ml.Be that LH all has band when being higher than 25mIU/ml, and the color of band increase with the content of LH and corresponding intensification; All there is not band when being lower than 25mIU/ml and 0mIU/ml.

Two, prozone phenomenon is observed: with negative urine LH is mixed with 100 respectively, 200,300IU/ml concentration found that under 100-300IU/ml concentration, it is existing that the paper slip of having a try all has red color area to take out of, illustrates that the structure setting of this test strips got rid of the interference of Hooke phenomenon.

Three, cross reaction: the short ovarian follicle of glycoprotein hormones is generated plain (FSH) add in the urine sample that does not contain LH, make that its concentration reaches 0,100,250 respectively, 500IU/ml, this test strips measurement result is all negative, and illustrating when originally being determined at FSH concentration up to 500IU/ml does not still have cross reaction with this contraception test strips.FSH physiology peak value is 10.57mIU/ml in the general normal women of child-bearing age midcycle urine, therefore under physiological conditions, does not have the cross reaction that causes because of FSH is too high.

Four, interference test: following material is added to the urine that does not contain LH or contains in the urine of LH200mIU/ml and tests.Interfering material comprises: acetylsalicylic acid, and concentration is 200mg/L, estradiol 14mg/L, protein 20g/L, glucose 2g/dl, haemoglobin 250g/dl, cholerythrin 10g/dl, triglyceride 970mg/dl, and change PH5 to PH9.Every group of test done three parts.The gained result is: all samples that does not contain LH no matter whether contain interfering material, are negative findings; All samples that contains LH200mIU/ml no matter whether contain interfering material, are positive findings, illustrate that they all do not have obvious interference to the mensuration of LH.

Five, storage stability: the sealing aluminium foil bag that test strips and dry bag will be housed is put respectively in 37 ℃ of incubators and the 4 ℃ of refrigerators, takes out every 2 days and 15 days respectively, and is each respectively with identical a positive sample and identical negative sample detection.Put 37 ℃ and preserve the measurement result of preserving 7 months in 1 month and 4 ℃ and to preserve preceding result consistent.

Embodiment 1: the preparation test strips, the corresponding antibodies that anti-LH monoclonal body H8 that the required main raw material(s) of this test paper is cultivated by Yunnan University and G6 cell line are produced is a main source, gold mark probe is selected the 3H8E3 of Yunnan University's preparation for use, during the assembling test strips, choose long for 100mm, wide for 80mm, thickly be the white PVC plastic bottom board of 1mm, by double faced adhesive tape stick in the centre earlier long for 100mm, wide be the nitrocellulose filter of the G6 monoclonal antibody of 30mm, intrinsic anti-LH; Again thereon section stick long for 100mm, widely be the thieving paper of 30mm, as filter paper for oil etc.; Its hypomere then stick long for 100mm, widely be the all-glass paper of 30mm, and on folder between the lower end of the upper end of this paper and nitrocellulose filter long for 100mm, wide for 10mm, be added with in advance and the gold of dried intrinsic anti-H8 is marked the glass fibre element film of probe; Carry out sealing with adhesive sticker respectively again at the two ends up and down of test strips then, the test strips that is cut into 80mm * 2mm afterwards again gets final product.This product through first affiliated hospital of Kunming medical college, Yunnan Province red cross hospital and first people of Yunnan Province adopt this test paper to 120 examples totally 1200 parts of normal women of child-bearing age midcycle urine samples measure, the result shows that this LH test paper and clinical verification degree of conformity reach 99.2%.Test method: (1) chooses the age the women of child-bearing age in 18-45 year, but must be not adopt the steroid contraception medical instrument in three months, does not do induced abortion in six months, not fertility in a year, and the women is volunteered in health check-up normally; (2) each experimenter of Can Jiaing all requires at test period, by its menstrual cycle length, respectively at urinating the first time that the time of appointment collects after getting up every day, it is urina sanguinis, it needs to collect 10 days continuously, the urine of collecting every day needs quick-frozen immediately in refrigerator, go to again after a while preserve in-20 ℃ of refrigerators stand-by; (3) select blank urine for use and contain Different L H concentration (10,25,50,100, urine 200mlU/ml), do not detect this test strips colour developing situation under blank, feminine gender and positive: later promptly from menstruation, use one every day, an end that will have the test strips of arrow inserts above-mentioned urine respectively, takes out after 10 seconds, observations after 5 minutes: measured the red display line always and occurred, just indication enters the onset of ovulation; Measure again when display line is red to disappear, and when measuring first always colour band and manifest, can stop using, and before at this moment come be absolute " safe period " during this period of time to next menstruation from continuous 4 days of this day; Treat that current menstruation carries out later the same test of following one-period.(4) contrast is selected luteinizing principle (LH) the ria-determination kit that Beijing biotechnology research produced for use (lot number: RH-035-100) carry out the mensuration of 1200 parts of urine samples, concrete assay method and computing method is carried out according to the experimental arrangement that accords with in the kit; (5) experimental result and statistical study: a. obtains 120 examples totally 1200 parts of urine samples in collection, and after women's " safe period " contraception test strips test and the test of LH radioimmunological kit, its result is a table 1:

Table: LH detects test paper and LH is put inspection-free test agent box clinical examination result:

Group	Umber	LH detects test paper		The LH radioimmunological kit
						Positive	Negative	Positive	Negative
		Ovulatory cycle is arranged	?97	??96	??1					??97	??0
Anovulatory cycle	?23					??0	??23	??0	??23

Compare by putting the agent Force orange survey result that is excused from an examination with LH, as can be known: b. is comparison sheet 2 as a result:

Group	Ovulatory cycle is arranged		Anovulatory cycle
					Positive	Negative	Positive	Negative
	LH is put inspection-free survey LH detection paper result as a result	??97 ??96	??0 ??1	??0 ??0					??23 ??23
The sensitivity of LH detection paper					??96/97＝99％
	LH detection paper specificity			??23/23＝100％
With clinical degree of conformity						??(96+23)/(97+23)＝99.2％

From above clinical verification 120 mid-term routine normal women of child-bearing age menstrual cycle urine sample not, wherein through LH radio-immunity kit checking normal ovulation person being taken place has 97 examples, belonging to the anovulatory cycle person then has 23 examples.In this 120 example, 23 example persons' anovulatory cycle survey through the LH test strip and belong to negative patient totally 23 examples, and the 97 examples person that has the ovulatory cycle through the test of LH test strip positive person to be arranged be 96 examples, so when checking LH to ovulate to indicate with the LH test strip, itself and clinical coincidence rate can reach 99.2%.

Claims (4)

1, a kind of safe period test paper detection method, it is characterized in that: utilize immunochromatography single stage method principle, special anti-LH monoclonal antibody and another anti-LH gold are marked a certain contiguous area band that probe is individually fixed in nitrocellulose filter, utilization from menstruation immerse every day later the urine sample of this film corresponding LH antigen can with anti-LH gold mark probe antibody generation specific bond after and by anti-LH monoclonal antibody identification, the specific bond of sandwich style promptly takes place, thereby by measuring the content of the lutropin LH in the urine, can measure women of child-bearing age's LH peak value per month, accurately to determine its corresponding onset of ovulation, the gold mark probe that promptly is stranded in this zone will show corresponding red, when the red display line occurs, just indication enters the onset of ovulation, when continuous 2 days of the same day was measured and not have colour band always and manifest during from the red display heading line off, before to next menstruation was absolute " safe period " during this period of time.

2, a kind of safe period test paper, it comprises base plate (7), it is characterized in that: on base plate (7), be fixed with the fine plain rete (5) of the nitric acid of anti-LH monoclonal antibody, be fixed with one deck absorbent paper layer (6) and glass fiber paper layer (1) at the two ends of this rete (5) respectively, and on folder between glass fiber paper layer (1) and the nitrocellulose filter (5), have the plain rete (2) of glass fibre of anti-LH Au probe.

3, by the detection method of claim 1 test paper of described safe period, it is characterized in that: bring into use the test paper inspection later from menstruation, use one every day, measured the red display line always and occurred, just indication enters the onset of ovulation; Measure again when display line is red to disappear, and measured when not having colour band always and manifesting from continuous 2 days of this day, can stop using, and before at this moment come to next menstruation be absolute " safe period " during this period of time, promptly (during 25mIU/ml, all do not have colour band and manifest, equal color band manifests during LH＞25mIU/ml when recording LH, and the concentration of LH is high more, and the colour band on the test strips is also just dark more.

4, by the described employing domestic type of the claim 1 LH monoclonal antibody ovulation tests detection paper method of the onset of ovulation, it is characterized in that: cellulose nitrate rete (5) is provided with p-wire (3) and control line (4).

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