CN109957139A - A kind of post-processing approach for nitrocellulose membrane - Google Patents
A kind of post-processing approach for nitrocellulose membrane Download PDFInfo
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- CN109957139A CN109957139A CN201910194326.5A CN201910194326A CN109957139A CN 109957139 A CN109957139 A CN 109957139A CN 201910194326 A CN201910194326 A CN 201910194326A CN 109957139 A CN109957139 A CN 109957139A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J9/00—Working-up of macromolecular substances to porous or cellular articles or materials; After-treatment thereof
- C08J9/36—After-treatment
- C08J9/40—Impregnation
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2301/00—Characterised by the use of cellulose, modified cellulose or cellulose derivatives
- C08J2301/08—Cellulose derivatives
- C08J2301/16—Esters of inorganic acids
- C08J2301/18—Cellulose nitrate
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- Separation Using Semi-Permeable Membranes (AREA)
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Abstract
The invention discloses a kind of post-processing approach for nitrocellulose membrane, sufficiently dry including carrying out hydrophobic nitrocellulose membrane to be processed；Hydrophobic nitrocellulose membrane after drying is put into hydrophilic treated solution and carries out continuous dipping processing or spray process is carried out to it using hydrophilic treated solution；The hydrophilic treated solution is alkyl polyglucoside surfactant solution；The nitrocellulose membrane that can solve " path " problem after sufficiently drying is carried out at 20 DEG C -75 DEG C.The present invention not only may insure to examine product testing line edge fastly clearly have without diffusion and climb plate speed faster, it is often more important that can substantially weaken or eliminate " the path phenomenon " examining occur in product fastly, guarantee the accuracy rate for examining product fastly.
Description
Technical field
Present invention relates particularly to a kind of post-processing approach for nitrocellulose membrane.
Background technique
Nitrocellulose is the product of cellulose Yu nitric acid esterification, has good film forming, is earliest in the world
It is used to prepare one of the high molecular polymer of film.In recent years, due to the macromolecule feature of nitrocellulose itself, application by
Gradually from be separated by filtration be transferred to higher bioanalysis of added value etc. application, this mainly has benefited from nitrocellulose to egg
The non-specific adsorption ability of the substances such as white matter, the preparation method of this kind of film are found in the Publication No. delivered
The Chinese invention patent application of CN103819710A.
On the other hand, since the application requirement film of quick diagnosis class product must have a hydrophily, and nitrocellulose
The hydrophobicity of itself proposes requirement to the hydrophiling post-processing of film again.Different hydrophilic treateds can generate the performance of film
Strong influence, to change the product performance of diagnostic products.
It often will appear " path " phenomenon in colloidal gold quick diagnosis product, be in product film colour developing pillar location two
There is sharp keen filament in side, so that being easy user is allowed to be mistaken for false positive leads to serious product quality problem." path "
Occur having very big relationship with the antigen-antibody of colloidal gold product label gold particle；But then, the position that " path " occurs
It is located exactly at the diffusion edge of antigen-antibody albumen coating buffer salt ion buffer band, due to the edge effect washed away and evaporated
It answers, diffusion edge is film hydrophilic agent and buffer salt ionic concentration highest point, shows that the hydrophilic agent of path and film is handled
There is very big association.It is therefore possible to can weaken or eliminate " path " phenomenon by specifically post-processing formula.
Summary of the invention
In view of the above-mentioned problems, the present invention proposes a kind of post-processing approach for nitrocellulose membrane, it can reduce and disappear
Except " path " problem in colloidal gold diagnosis product.
In order to achieve the above technical purposes, reach above-mentioned technical effect, the invention is realized by the following technical scheme:
A kind of post-processing approach for nitrocellulose membrane, comprising the following steps:
Hydrophobic nitrocellulose membrane to be processed is carried out sufficiently dry；
Hydrophobic nitrocellulose membrane after drying is put into hydrophilic treated solution and carries out continuous dipping processing or utilization
Hydrophilic treated solution carries out spray process to it；The hydrophilic treated solution is alkyl polyglucoside surfactant solution；
Sufficiently drying is carried out at 20 DEG C -75 DEG C can solve the nitrocellulose membrane of " path " problem.
As a further improvement of the present invention, the hydrocarbyl group of the alkyl polyglycoside surface activating solution contains 6-20
Carbon atom.
As a further improvement of the present invention, the hydrocarbyl group of the alkyl polyglycoside surface activating solution contains 8-18
Carbon atom.
As a further improvement of the present invention, the preparation process of the hydrophilic treated solution are as follows:
One or more alkyl polyglycoside surface activating solutions are added into deionized water, one or more alkyl are more
The total accounting of quality for the mixture that polyglycoside surfactant living solution is formed is 0.01-1%；
It is stirred evenly at 10-60 DEG C, stands after eliminating bubble, obtain the hydrophilic treated solution.
As a further improvement of the present invention, the hydrophobic nitrocellulose membrane by after drying is put into hydrophilic treated solution
The middle soaking time for carrying out continuous dipping processing is 0.1-30 minutes.
As a further improvement of the present invention, the hydrophobic nitrocellulose membrane by after drying is put into hydrophilic treated solution
The middle soaking time for carrying out continuous dipping processing is 0.2-20 minutes.
As a further improvement of the present invention, the aperture of the hydrophobic nitrocellulose membrane is 6-12um.
Compared with prior art, beneficial effects of the present invention:
A kind of post-processing approach for nitrocellulose membrane proposed by the present invention, not only may insure to examine product testing fastly
Line edge has clearly without diffusion and climbs plate speed faster, it is often more important that can substantially weaken or eliminate and examine in product fastly
" the path phenomenon " occurred guarantees the accuracy rate for examining product fastly, while having de- plate faster using the film that this method obtains
Tomography Velocity allows colloidal gold product faster to carry out reading measurement to guarantee that background quickly elutes completely.
Detailed description of the invention
Fig. 1 is the flow diagram of the post-processing approach for nitrocellulose membrane of an embodiment of the present invention；
Fig. 2 is the surface texture schematic diagram of hydrophobic nitrocellulose membrane employed in an embodiment of the present invention；
Fig. 3 is the surface texture schematic diagram of the product of comparative example preparation in an embodiment of the present invention；
Fig. 4 is the detection comparison diagram of embodiment product and contrast product in an embodiment of the present invention.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
It limits the scope of protection of the present invention.
Application principle of the invention is explained in detail with reference to the accompanying drawing.
The preparation method of hydrophobic nitrocellulose membrane mentioned in the present invention is found in the Publication No. delivered
The Chinese invention patent application of CN103819710A, the present invention involved in hydrophobic nitrocellulose membrane i.e. publication number
Film disclosed in Chinese invention patent application for CN103819710A.
As shown in Figure 1, providing a kind of post-processing approach for nitrocellulose membrane in the present invention, specifically include following
Step:
(1) hydrophobic nitrocellulose membrane to be processed is subjected to sufficiently dry and dedusting；
In a specific embodiment of the invention, the hydrophobic nitrocellulose membrane to be processed be without appoint
The hydrophobic nitrocellulose membrane of where reason and dedusting, the aperture of the hydrophobic nitrocellulose membrane are 6-12um；
(2) the hydrophobic nitrocellulose membrane after drying is put into hydrophilic treated solution and carries out continuous dipping processing or benefit
Spray process is carried out to it with hydrophilic treated solution, and guarantees spray sufficiently, i.e., all surface in hydrophobic nitrocellulose membrane
Product is arrived by spray；The hydrophilic treated solution is alkyl polyglucoside surfactant solution；
In a specific embodiment of the invention, the hydrophobic nitrocellulose membrane by after drying is put into hydrophilic treated
The soaking time that continuous dipping processing is carried out in solution is 0.1-30 minutes, preferably 0.2-20 minutes；
In a specific embodiment of the invention, the preparation process of the hydrophilic treated solution are as follows:
One or more alkyl polyglycoside surface activating solutions are added into deionized water, one or more alkyl are more
The total accounting of quality for the mixture that polyglycoside surfactant living solution is formed is 0.01-1%；
It is stirred evenly at 10-60 DEG C, stands after eliminating bubble, obtain the hydrophilic treated solution；
Wherein, the hydrocarbyl group of the alkyl polyglycoside surface activating solution contains 6-20 carbon atom, preferably 8-18
A carbon atom；
(3) sufficiently drying is carried out at 20 DEG C -75 DEG C can solve the nitrocellulose membrane of " path " problem.
The nitrocellulose membrane obtained by above-mentioned steps is tested below, detailed process are as follows:
1) nitrocellulose membrane for cutting one section of 4cm long observes and records time of the distilled water from bottom chromatography to top；
2) one section is taken to pass through above-mentioned steps 1) processed nitrocellulose membrane, and it is quick to apply to double-antibody sandwich hCG
Diagnostic products carry out antibody protein point film, and carry out test sample, shoot and record test features quality.
Embodiment 1:
The embodiment of the invention provides a kind of post-processing approach for nitrocellulose membrane, specifically include:
Hydrophilic treated solution is prepared, quality composition is as follows: lauryl glucoside 0.15%, remaining group are divided into deionization
Water stirs evenly set aside for use.The hydrophobic nitrocellulose membrane for drying dedusting in advance is placed in the hydrophilic treated solution
Middle progress continuous dipping processing, soaking time 5min, film are dried at 50 DEG C.Films test the results are shown in Table 2 and Fig. 4
In (a), film surface structure is shown in Fig. 2；
Embodiment 2:
The processing step that embodiment 1 prepares film is repeated, hydrophilic treated solution is n-hexyl glucoside, and concentration is
0.03%, film is dried at 30 DEG C.Films test the results are shown in Table (b) in 2 and Fig. 4.
Embodiment 3:
The processing step that embodiment 1 prepares film is repeated, hydrophilic treated solution is the mixing of oligomeric C8-C16 glucose alkylglycoside
Liquid, total concentration 0.20%, soaking time 20min.Films test the results are shown in Table (c) in 2 and Fig. 4.
Comparative example 1:
The step of embodiment 1 prepares film is repeated, hydrophilic treated solution is lauryl sodium sulfate, and concentration is
0.15%.Films test the results are shown in Table (d) in 2 and Fig. 4.
Comparative example 2:
Certain import cellulose nitrate film is taken to carry out equal conditions test.Surface texture is shown in Fig. 3, test result
(e) being shown in Table in 2 and Fig. 4.
It is fine that hydrophily nitrification in EXPERIMENTAL EXAMPLE 1 is repeated by the nitrocellulose solution constituent content specified in following table 1
The preparation method for tieing up plain film, respectively obtains different hydrophily nitrocellulose membrane products.
Table 1
By embodiment 1 into embodiment 3 preparation method product 1 obtained to product 3, with existing comparative example to product 1,
Product 2 are compared, experimental result is shown in Fig. 2 to Fig. 4, data are as shown in table 2:
Table 2
It can see and find from 2 test result of table, in the case of identical membrane aperture, alkyl polyglucoside surfactant can be mentioned
The Tomography Velocity for rising film can be more adaptive to hCG early pregnancy class product to the fast demand for reading reading.And from the comparison knot of Fig. 4
From the point of view of fruit, in same specific lot number antibody, product 1-3 equal clean background of T line position in negative test is noiseless；
And have the presence of a small amount of sharp light filament in T line upper and lower side to product 1 and to product 2, as " path ", it be easy to cause the false sun of product
Erroneous judgement.It can be seen that the film using post-processing approach as described herein is wider to the selectivity of antibody, it can be to avoid " rail
The appearance of line " problem.
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (7)
1. a kind of post-processing approach for nitrocellulose membrane, which comprises the following steps:
Hydrophobic nitrocellulose membrane to be processed is carried out sufficiently dry；
Hydrophobic nitrocellulose membrane after drying is put into hydrophilic treated solution and carries out continuous dipping processing or utilizes hydrophilic
Processing solution carries out spray process to it；The hydrophilic treated solution is alkyl polyglucoside surfactant solution；
The nitrocellulose membrane that can solve " path " problem after sufficiently drying is carried out at 20 DEG C -75 DEG C.
2. a kind of post-processing approach for nitrocellulose membrane according to claim 1, it is characterised in that: the alkyl
The hydrocarbyl group of polysaccharide glycosides surface activating solution contains 6-20 carbon atom.
3. a kind of post-processing approach for nitrocellulose membrane according to claim 2, it is characterised in that: the alkyl
The hydrocarbyl group of polysaccharide glycosides surface activating solution contains 8-18 carbon atom.
4. a kind of post-processing approach for nitrocellulose membrane according to any one of claim 1-3, feature exist
In: the preparation process of the hydrophilic treated solution are as follows:
One or more alkyl polyglycoside surface activating solutions, one or more alkyl polyglycoside are added into deionized water
The total accounting of quality for the mixture that surface activating solution is formed is 0.01-1%；
It is stirred evenly at 10-60 DEG C, stands after eliminating bubble, obtain the hydrophilic treated solution.
5. a kind of post-processing approach for nitrocellulose membrane according to claim 1, it is characterised in that: described to do
Hydrophobic nitrocellulose membrane after dry, which is put into hydrophilic treated solution, carries out the soaking time of continuous dipping processing as 0.1-30 points
Clock.
6. a kind of post-processing approach for nitrocellulose membrane according to claim 5, it is characterised in that: described to do
Hydrophobic nitrocellulose membrane after dry, which is put into hydrophilic treated solution, carries out the soaking time of continuous dipping processing as 0.2-20 points
Clock.
7. a kind of post-processing approach for nitrocellulose membrane according to claim 1, it is characterised in that: described hydrophobic
The aperture of nitrocellulose membrane is 6-12um.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910194326.5A CN109957139A (en) | 2019-03-14 | 2019-03-14 | A kind of post-processing approach for nitrocellulose membrane |
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| CN201910194326.5A CN109957139A (en) | 2019-03-14 | 2019-03-14 | A kind of post-processing approach for nitrocellulose membrane |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116731563A (en) * | 2023-05-22 | 2023-09-12 | 北京北方世纪纤维素技术开发有限公司 | Flexible modified nitrocellulose casting solution, nitrocellulose membrane, preparation method and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1262439A (en) * | 1999-02-03 | 2000-08-09 | 昆明云大生物技术有限公司 | Contraceptive test paper for safe period and its test method |
| WO2003102246A1 (en) * | 2002-05-31 | 2003-12-11 | Penn State Research Foundation | Dot-elisa for the detection of animal viruses |
| CN102077091A (en) * | 2008-06-30 | 2011-05-25 | 积水医疗株式会社 | Porous solid phase for binding assay, and binding assay method using the same |
| CN103819710A (en) * | 2014-03-04 | 2014-05-28 | 苏州奥特福环境科技有限公司 | Method for preparing hydrophilic nitrated cellulose film |
| CN106918698A (en) * | 2015-12-25 | 2017-07-04 | 广州瑞博奥生物科技有限公司 | A kind of phospho-AB chip agent box for detecting human receptor tyrosine kinase enzyme |
| CN107328941A (en) * | 2017-06-24 | 2017-11-07 | 梧州市兴能农业科技有限公司 | It is a kind of to detect the antibody chip of various kinds of cell adhesion factor simultaneously |
-
2019
- 2019-03-14 CN CN201910194326.5A patent/CN109957139A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1262439A (en) * | 1999-02-03 | 2000-08-09 | 昆明云大生物技术有限公司 | Contraceptive test paper for safe period and its test method |
| WO2003102246A1 (en) * | 2002-05-31 | 2003-12-11 | Penn State Research Foundation | Dot-elisa for the detection of animal viruses |
| CN102077091A (en) * | 2008-06-30 | 2011-05-25 | 积水医疗株式会社 | Porous solid phase for binding assay, and binding assay method using the same |
| CN103819710A (en) * | 2014-03-04 | 2014-05-28 | 苏州奥特福环境科技有限公司 | Method for preparing hydrophilic nitrated cellulose film |
| CN106918698A (en) * | 2015-12-25 | 2017-07-04 | 广州瑞博奥生物科技有限公司 | A kind of phospho-AB chip agent box for detecting human receptor tyrosine kinase enzyme |
| CN107328941A (en) * | 2017-06-24 | 2017-11-07 | 梧州市兴能农业科技有限公司 | It is a kind of to detect the antibody chip of various kinds of cell adhesion factor simultaneously |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116731563A (en) * | 2023-05-22 | 2023-09-12 | 北京北方世纪纤维素技术开发有限公司 | Flexible modified nitrocellulose casting solution, nitrocellulose membrane, preparation method and application thereof |
| CN116731563B (en) * | 2023-05-22 | 2024-03-01 | 北京北方世纪纤维素技术开发有限公司 | Flexible modified nitrocellulose casting solution, nitrocellulose membrane, preparation method and application thereof |
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