CN1254440C - Method for preparing granular ammonia oxidative mud with anaerobic bacteria from mixed mud - Google Patents
Method for preparing granular ammonia oxidative mud with anaerobic bacteria from mixed mud Download PDFInfo
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- CN1254440C CN1254440C CNB200410042793XA CN200410042793A CN1254440C CN 1254440 C CN1254440 C CN 1254440C CN B200410042793X A CNB200410042793X A CN B200410042793XA CN 200410042793 A CN200410042793 A CN 200410042793A CN 1254440 C CN1254440 C CN 1254440C
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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Abstract
The present invention relates to a method for preparing granular ammonia oxidative mud with anaerobic bacteria from mixed mud, which belongs to the technical field of biological wastewater treatment and aims to solve the problems of the low volume load, the low removal rate, etc. of a reactor due to low sludge concentration in the existing anaerobic ammonia oxidation reactor. The present invention discloses a novel cultural method for anaerobic granular ammonia oxidative mud, which comprises the following steps: common granular anaerobic mud and active aerobic mud are uniformly mixed and are put into an upflow anaerobic reactor; simulated sewage is compounded and is pumped from the bottom of the reactor; the sewage flows out from the upper part of the reactor after flowing across the mixed mud, and partial effluent water flows back; the ascending velocity of the water power of the reactor is controlled between 1 m/h and 2 m/h; under the condition of keeping the temperature, the pH value, etc. in the reactor, after the stable operation is lasted for a period of time, the granular mud is obtained. The anaerobic granular ammonia oxidative mud cultured by the present invention can make the volume load, the removal efficiency, the operating stability, etc. of the reactor greatly enhanced.
Description
Technical field
The present invention relates to a kind of method of utilizing mixing sludge to cultivate anaerobic ammonium oxidation granular sludge, belong to the biological wastewater treatment technology field.
Background technology
Along with the aggravation of nitrate pollution and the enhancing of people's environmental protection consciousness, the wastewater biological denitrificaion technology has caused the common concern of countries in the world, becomes the important research direction of water pollution control.The ultimate principle of traditional biological denitrogenation is by nitration reaction ammonia nitrogen to be oxidized to nitrate earlier under aerobic condition, becomes gaseous nitrogen to overflow from water nitrate reduction by the anoxic denitrification reaction under organism participates in again.In this process, the nitrogen transformation path is very long, causes the power consumption of traditional biological denitrification process excessive, and needs organic participation, has increased the working cost and the complexity of technology.By comparison, the anaerobic ammonia oxidation process of discovered in recent years can utilize anaerobic ammonium oxidizing bacteria with NO under anaerobism or anoxia condition
2 -Be electron acceptor(EA), with NH
4 +Direct oxidation is nitrogen (N
2), be the simplest and the most direct biological denitrification process of having found at present, it is low and need not to add organic advantage to have energy consumption.But, because anaerobic ammonium oxidizing bacteria is a growth autotrophic bacteria very slowly, it it be unclear that in natural characteristic distributions, thereby ubiquity problems such as load is lower, clearance is on the low side, operational conditions requirement harshness in the anaerobic ammonia oxidation reactor of successful operation at present.Granule sludge is that a large amount of bacteriums assemble the particulate state microbial aggregate with good settling property that growth forms, and granule sludge is key and the prerequisite that realizes efficient operation in high efficiency anaerobic reactor such as UASB (upflow type anaerobic reactor), EGSB (expanded granular bed bioreactor) and IC (anaerobism inner circulation reactor) etc.In anaerobic ammonia oxidation reactor, turn out anaerobic ammonium oxidation granular sludge, can improve the biomass in the reactor, improve operation stability and treatment efficiency with greater activity.
Summary of the invention
The objective of the invention is to hang down and problems such as clearance is low in order to solve the reactor volume load that causes owing to sludge concentration is low in the existing anaerobic ammonia oxidation reactor.At the problems referred to above, the invention provides a kind of anaerobic ammonium oxidation granular sludge and its cultural method.
The method of utilizing mixing sludge to cultivate anaerobic ammonium oxidation granular sludge disclosed by the invention is characterized in that this method comprises the steps:
1) chooses common anaerobic grain sludge and aerobic activated sludge respectively, and the two is mixed, pack into and be furnished with the upflow type anaerobic reactor of triphase separator;
2) preparation contains ammonia nitrogen and nitrous acid nitrogen, does not contain the simulated wastewater of organic carbon source, and ammonia nitrogen concentration is 200～800mg/L in the described simulated wastewater, and the ratio of ammonia nitrogen and nitrous acid nitrogen is between 0.7～1.2; In simulated wastewater, also need add the normal growth that an amount of phosphoric acid salt, trace element and yeast extract guarantee microorganism;
3) bottom from described reactor pumps into described simulated wastewater, and waste water is flowed through and flowed out from reactor top behind the described mixing sludge, and the part effluent recycling is controlled at the waterpower upflow velocity of reactor between 1～2m/h;
4) keep the interior temperature of reactor between 28～33 ℃, the pH value between 7.5～8.5, the redox potential value 1～-50mv between, hydraulic detention time is 8～14 hours, after steady running for some time, in reactor, obtain anaerobic ammonium oxidation granular sludge.
In the present invention, the blending ratio of described common anaerobic grain sludge and aerobic activated sludge is preferably (1～10): 1, and preferred blending ratio is (1～2): 1.
In the present invention, described trace element is one or more among Zn, Co, Mn, Cu, Mo, Ca and the Fe, and every kind of trace element is 0.0005～0.005g/L in the concentration of simulated wastewater.
In the present invention, described phosphoric acid salt is K
2HPO
4, KH
2PO
4, Na
2HPO
4And NaH
2PO
4In one or more, phosphoric is 0.01～0.1g/L in the concentration of simulated wastewater.
In the present invention, the concentration of yeast extract in simulated wastewater is 0.01～0.1g/L.
The anaerobic ammonium oxidation granular sludge of turning out according to the method for the invention has superperformance, and the aspects such as volumetric loading, removal efficient and operation stability of reactor all are greatly improved.
Embodiment
At first common anaerobic grain sludge and aerobic activated sludge are mixed, common anaerobic grain sludge and aerobic activated sludge blending ratio are preferably (1～10): 1, and preferred ratio is (1～2): 1.Then mixed soil is packed into and be furnished with in the upflow type anaerobic reactor of triphase separator, the effect of the triphase separator cotton-shaped mud in the seed sludge that is to dam.The simulated wastewater that employing contains ammonia nitrogen and nitrous acid nitrogen starts reactor, ammonia nitrogen concentration is 200～800mg/L in the waste water, the ratio control of ammonia nitrogen and nitrous acid nitrogen is between 0.7～1.2, in simulated wastewater, also added an amount of phosphoric acid salt, trace element and yeast extract are to guarantee the normal growth of microorganism, phosphoric is 0.01～0.1g/L in the concentration of simulated wastewater, Zn, Co, Mn, Cu, Mo, trace element such as Ca and Fe is 0.0005～0.005g/L in the concentration of simulated wastewater, yeast extract is 0.01～0.1g/L in the concentration of simulated wastewater, does not contain organic carbon source in the waste water.Pump into described simulated wastewater from the bottom of described reactor, waste water is flowed through and is flowed out from reactor top behind the described mixing sludge, the part effluent recycling is controlled at the waterpower upflow velocity of reactor between 1～2m/h, has certain rate of expansion to guarantee the mud bed.For guaranteeing the steady running of reactor, following operating parameter is controlled: 28～33 ℃ of temperature, pH value 7.5～8.5, ORP value-100～-50mv, hydraulic detention time 8～14 hours after steady running for some time, just can obtain anaerobic ammonium oxidation granular sludge in the reactor.
Embodiment 1
Even the aerobic activated sludge in certain CASS pond of anaerobic grain sludge in certain UASB reactor of brewery wastewater treatment and processing sanitary sewage by 1: 1 mixed.Then mixed soil is inoculated in the upflow type anaerobic reactor, the cumulative volume of this reactor is 3.05L, and wherein the reaction zone volume is 1.60L, and settling region 1.04L is furnished with triphase separator in the settling region, and the sludge concentration in the inoculation post-reactor is 13.2gSS/L.Ammonia nitrogen concentration is 200～600mg/L in the simulated wastewater, and ammonia nitrogen is 0.7 with the ratio of nitrous acid nitrogen, has also added KH in the above-mentioned in addition waste water
2PO
4, trace element and yeast extract, concentration is respectively 0.05gP/L, 0.001g Zn/L, 0.0005g Co/L, 0.002g Mn/L, 0.0008g Cu/L, 0.0012g Mo/L, 0.003g Ca/L, 0.002g Fe/L and 0.05g yeast extract/L, does not contain organic carbon source in the simulated wastewater.The operational condition of reactor is: 29～31 ℃ of temperature, and pH value 7.9～8.3, hydraulic detention time 8～12 hours, the ORP in the reactor be controlled at-80～-50mv, the waterpower upflow velocity is controlled at 1～2m/h.Steady running was observed tan anaerobic ammonium oxidation granular sludge in the post-reactor of the left and right sides in 200 days.The main particle size range of granule sludge is 0.4～1.2mm, wherein 0.6～1.0mm account for 55.8%, account for 20.9% greater than 1.0mm; The settling velocity average out to 53.5m/h of granule sludge; Anammox speed is about 0.2kgN/ (kgVSSd).
The anaerobic ammonium oxidation granular sludge of turning out is put into another upflow type anaerobic reactor treatment of simulated waste water, at total nitrogen volumetric loading 2.5kg/ (m
3D) under the condition, the clearance of ammonia nitrogen and nitrous acid nitrogen all can reach more than 95%, and this reactor serviceability is good, and has advantages of higher stability.
Embodiment 2
Even the aerobic activated sludge in certain aeration tank of anaerobic grain sludge in certain EGSB reactor of handling starch wastewater and processing sanitary sewage by 10: 1 mixed.All the other operating process are identical with embodiment 1, and cultured continuously anaerobic ammonium oxidation granular sludge occurred in the post-reactor of the left and right sides in 320 days.
Embodiment 3
Adopt the seed sludge identical, KH with embodiment 1
2PO
4, trace element and yeast extract concentration be 0.01g P/L, 0.001g Co/L, 0.003g Mn/L, 0.002g Mo/L, 0.002g Ca/L, 0.003g Fe/L and 0.01g yeast extract/L.All the other operating process are identical with embodiment 1, and cultured continuously anaerobic ammonium oxidation granular sludge occurred in the post-reactor of the left and right sides in 280 days.
Claims (2)
1. utilize mixing sludge to cultivate the method for anaerobic ammonium oxidation granular sludge, it is characterized in that this method comprises the steps:
1) chooses common anaerobic grain sludge and aerobic activated sludge respectively, and the two is mixed, pack into and be furnished with the upflow type anaerobic reactor of triphase separator; The blending ratio of described common anaerobic grain sludge and aerobic activated sludge is (1～10): 1;
2) preparation contains ammonia nitrogen and nitrous acid nitrogen, does not contain the simulated wastewater of organic carbon source, and ammonia nitrogen concentration is 200～800mg/L in the described simulated wastewater, and the ratio of ammonia nitrogen and nitrous acid nitrogen is between 0.7～1.2; In simulated wastewater, also need add the normal growth that an amount of phosphoric acid salt, trace element and yeast extract guarantee microorganism, trace element is one or more among Zn, Co, Mn, Cu, Mo, Ca and the Fe, every kind of trace element is 0.0005～0.005g/L in the concentration of simulated wastewater, and phosphoric acid salt is K
2HPO
4, KH
2PO
4, Na
2HPO
4And NaH
2PO
4In one or more, phosphoric is 0.01～0.1g/L in the concentration of simulated wastewater; The concentration of yeast extract in simulated wastewater is 0.01～0.1g/L;
3) bottom from described reactor pumps into described simulated wastewater, and waste water is flowed through and flowed out from reactor top behind the described mixing sludge, and the part effluent recycling is controlled at the waterpower upflow velocity of reactor between 1～2m/h;
4) keep the interior temperature of reactor between 28～33 ℃, the pH value between 7.5～8.5, the redox potential value 1～-50mv between, hydraulic detention time is 8～14 hours, steady running-section obtains anaerobic ammonium oxidation granular sludge after the time in reactor.
2. the method for utilizing mixing sludge to cultivate anaerobic ammonium oxidation granular sludge according to claim 1 is characterized in that: the blending ratio of described common anaerobic grain sludge and aerobic activated sludge is (1～2): 1.
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| CNB200410042793XA CN1254440C (en) | 2004-05-28 | 2004-05-28 | Method for preparing granular ammonia oxidative mud with anaerobic bacteria from mixed mud |
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|---|---|---|---|
| CNB200410042793XA CN1254440C (en) | 2004-05-28 | 2004-05-28 | Method for preparing granular ammonia oxidative mud with anaerobic bacteria from mixed mud |
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| CN1583600A CN1583600A (en) | 2005-02-23 |
| CN1254440C true CN1254440C (en) | 2006-05-03 |
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Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100404436C (en) * | 2005-06-03 | 2008-07-23 | 清华大学 | Culture method of aerobic nitrosated granular sludge |
| CN100378008C (en) * | 2005-10-08 | 2008-04-02 | 钱俊 | High-concentration ammonia oxidation waste water treatment |
| CN100400650C (en) * | 2006-02-14 | 2008-07-09 | 浙江大学 | Separation and authentication method for Planctomyces with anaerobic ammoxidation activity |
| CN102190371B (en) * | 2010-03-18 | 2013-01-30 | 华东师范大学 | Breeding method for anaerobic ammonium oxidation granular sludge |
| CN101838051B (en) * | 2010-04-02 | 2011-12-07 | 中煤能源黑龙江煤化工有限公司 | Anaerobic acclimation-culturing process and device for sludge |
| CN101805060B (en) * | 2010-04-23 | 2011-07-06 | 山东大学 | Method for fast realizing anaerobic ammonium oxidation granulation |
| CN102219300B (en) * | 2011-05-16 | 2012-11-21 | 苏州科技学院 | Method for culturing anaerobic granular sludge in anaerobic baffled reactor |
| CN102432097B (en) * | 2011-10-31 | 2013-11-20 | 北京桑德环境工程有限公司 | Method for quickly starting anaerobic ammonium oxidation in upflow packed bed reactor |
| CN102976485B (en) * | 2012-12-10 | 2013-12-04 | 东北电力大学 | Method and device for fast cultivation of anaerobic granular sludge for treating swine wastewater |
| CN103112948B (en) * | 2013-02-03 | 2014-09-24 | 北京工业大学 | Method for rapidly culturing autotrophic nitrogen removal granule sludge under conditions of low substrate concentration and high ascending velocity |
| CN105036321B (en) * | 2015-07-09 | 2017-05-03 | 杭州师范大学 | Method for culturing anammox sludge with high temperature resistance |
| CN106396096A (en) * | 2016-11-04 | 2017-02-15 | 苏州依斯倍环保装备科技有限公司 | Method and device for culturing anaerobic granular sludge |
| CN108358310A (en) * | 2018-01-09 | 2018-08-03 | 桂林理工大学 | A kind of EGSB anaerobic bio-treated methods of artificial stone's processing waste water |
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- 2004-05-28 CN CNB200410042793XA patent/CN1254440C/en not_active Expired - Fee Related
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Granted publication date: 20060503 Termination date: 20140528 |