CN1244771A - Agricultural compositions containing bacteria - Google Patents
Agricultural compositions containing bacteria Download PDFInfo
- Publication number
- CN1244771A CN1244771A CN 96180578 CN96180578A CN1244771A CN 1244771 A CN1244771 A CN 1244771A CN 96180578 CN96180578 CN 96180578 CN 96180578 A CN96180578 A CN 96180578A CN 1244771 A CN1244771 A CN 1244771A
- Authority
- CN
- China
- Prior art keywords
- composition
- bacillus
- plant growth
- described reinforcement
- active component
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
An agriculturally effective active ingredient is applied to plant foliage before, after, or simultaneously with an enhancer component containing a substantially pure bacterial culture, suspension, spores, or cells of a bacteria selected from the genus Bacillus or a soil bacteria.
Description
Invention field
The present invention relates to a kind of compositions-treated plant, said composition contains effective active component and a kind of enhancer additives that contains the pure substantially culture of the bacterium that is selected from bacillus or soil bacteria on a kind of agricultural.The culture that is added can be cell, brood cell or form of suspension.
Background of invention
Pesticide producing merchant is seeking the way that improves the effectiveness of using the active component on plant always.When employed material was plant growth regulator (growth inhibition or growth), weed killer herbicide or inner absorbent (for example, insecticide or fungicide), special hope so.Enter the transport mechanism of plant, and the transfer between various plant tissues is very important, and under some occasion, may is the principal element of the effectiveness of the decision composition that uses.For some active component, the improvement of transport mechanism may show as the raising of effect under existing usage amount, needs active component still less, maybe can handle the ability of new species, and these species had resistance to this active component in the past.
Helping strong element is a kind of active component, and for this composition, absorption of plant or conveying are important.Help strong plain (N, N-lupetidine cation chloride) to be used as active component and use once a year on up to a million acres cotton,, and strengthen the fruit retention effect so that suppress nourishing and growing of cotton plant.Help strong element that potato, sweet potato, peanut, grape, corn, wheat, oranges and tangerines, tomato and onion are also had some effect.
Help strong element that cotton plants is had the effect that inhibition is nourished and grown, thereby force this plant that its energy is used for fruit (cotton boll) production.The plant that begins to occur the overnutrition growth suitably used help strong plainly, can keep or improve the output of cotton plant, and can not damage this plant.When crop is grown in the fertile soil or helping needing described growth inhibition effect especially after the weather condition that plant strain growth rather than fruit form.
Cotton plants has foreseeable life cycle and vegetative period.Cotton seeds being planted in the trench after 7-10 days, grow cotton plants.The root growth of cotton plants and the plant height elongation of being undertaken by cane, and branch's growth is to carry out with the form that is called as " nourishing and growing ", this growth lasts till the 4-8 joint always.Then, cotton plant produces a reproduction branch (first " solid site "), and all branches subsequently all are genitalities.Cotton growers attempts to control the growth of plant, helps the expected range of reproductive growth with the ratio of reproductive growth (knot bell) to guarantee to nourish and grow.
Cotton growers likes generally seeing that the length between the stem joint is approximately 2 inches (5cm).This ratio mean undue reproductive growth (knot bell) or too nourish and grow between balance, excessive reproductive growth can cause the growth of plant to surpass the speed of nourishing and growing, and before output reaches maximum, finish growth, excessive nourishing and growing can be reduced the quantity of ripe cotton boll.
The cotton plant that can utilize plant energy to be used to nourish and grow major part is known as " overgrowing cotton ", and that this plant produces is less, subsequently can ripe cotton boll, and is subject to the influence of extreme weather in a long time.The cotton that shows the overgrowing sign can be at an easy rate by the unusual plant height of knot bell plant and the quantity identification of genitality stem joint relatively.Can will help strong element to be used to stop the overgrowing of cotton, this purpose be to realize by the growth characteristics that change cotton plants.
Generally, the branch of stem always stretches out from an alternately side of stem.Each branch sites is known as one " joint ", before the first setting branch that has true leaf forms, can form 5-7 joint on cotyledon.The counting of joint starts from the bottom of plant, and extends upward along stem." length of internode " was the distance between the branch point, approximately formed a new joint every 3 days.In order to measure and to compare, use above joint number and the length of internode of the 8th joint usually, so that eliminate the variation of setting joint between plant, because setting branch must be formed by the 8th joint.Therefore, the counting of setting joint is normally since the 1st gonosomite, and this gonosomite is generally the 7th or the 8th joint.
Setting site on the cotton plant is called as " square ".Each setting branch can form 1-6 site, common about 3 setting sites (" square "), and the time interval that forms square in each branch is approximately 6 days.The beginning of new square on the cotton plants and reproductive growth is known as " syringe needle " square, because its size hardly as seen.Approximately after 5-10 days, this square has grown to approximately near the match end size, and this stage is known as " match end square " in the cycle plant.The continued growth of match end square is near the normal adult human thumbnail, bloom then (" flower just ").After 3 days, formation cotton boll below flower.After first flower about 30 days, the product cotton boll is full maturity, and can gather.Generally speaking, about 80% of cotton gross yield is formation in 3 weeks after initial bloom stage, and gross yield 95% is to form within 5 weeks of initial bloom stage.
Generally, will help strong element to use on the cotton plants with a kind of in two kinds of methods.Till 1986, the method for Shi Yonging is that to give the percentage by weight of the every acre of disposable use 8-16 of cotton ounce at initial bloom stage be 4.2% solution always.The method of this disposable processing can be controlled the height of plant really, but, has found that plant products can reduce once in a while, when particularly plant is subjected to coercing during use or afterwards.
From later trend in 1987 be, by a series of should be used for using help strong plainly, the dosage of Shi Yonging all is lower than the dosage of single use each time.The 1st processing carried out in the match end square phase, carried out the 2nd time after 7-14 days and handled.The usage amount of twice processing all is 4.2% solution at every acre of about 0-8 ounce percentage by weight, concrete usage amount depends on whether cotton plants following sign occurs: coerced (not using), moderate growth (every acre of about 2 ounces of solution), or active growth (every acre of about 4 ounces of solution).Then, carry out twice processing again with 7-14 days intervals, it is 4.2% the strong cellulose solution that helps that usage amount can be brought up to about 8 ounces of percentage by weights, and concrete consumption depends on big Tanaka's the amount of nourishing and growing.By the further test that indivedual growers carry out, can draw various nonexpondable amounts.
If it will be favourable will helping strong plain use to be integrated into a treatment system, can improve the plant tissue quality in root, stem, the leaf like this, so that being provided, shifts higher levels of nutrient, and meanwhile, restriction is nourished and grown, to improve fruit yield.
The plant herbicide technology wishes to improve grass effectiveness extremely always, and don't the consumption of the corresponding increase weed killer herbicide of meeting.A lot of weed killer herbicides also can use a kind of accelerative activator, and do not improve the amount of employed weed killer herbicide.Farmer and weed killer herbicide manufacturer are faced with control weeds and noxious plant growth simultaneously and don't surpass the needs of the weed killer herbicide usage amount of verifying, if plant can be fundamentally controlled.Certain plants, as the Florida kitchen garden, the bull grass, Bermuda grass, dog fennel and primrose all are the high resistance weed killer herbicides, have confirmed these plants effective.
If have a kind of effectiveness that can improve such as the active component on the agricultural of weed killer herbicide, and don't the method that improves this absorption of active ingredient will be very useful.
Summary of the invention
An object of the present invention is to provide a kind of composition and using method, to improve the effectiveness that agricultural goes up effective active component.
Another object of the present invention is, a kind of composition is provided and said composition is used in method on the solid Plants and Seeds, and to increase the setting number of sites of handling on the plant, its target provides the fruit of high yield.
A further object of the present invention provides a kind of compoistion and method of use, and wherein, the plant of handling can be with more healthy state growth.
According to above-mentioned and other purpose (these purposes by this specification as can be seen) of the present invention, composition of the present invention comprises: (a) effective active component on a kind of agricultural, (b) a kind of reinforcement composition that contains culture, this culture contains the bacterium that comes from bacillus or soil bacteria of cell, culture or form of suspension, and its consumption is enough to improve the effect of described active component.Preferably, described reinforcement composition with do not contain auxin when helping strong plain plant growth inhibitor to be used in combination, help the strong plain auxin that can suppress in the processing plant.
Composition of the present invention can improve the effectiveness of the active component on the employed agricultural.Usually the active material of use same amount can be more effective.Use more a spot of active component can obtain same effect with higher conventional amount used.In addition, more healthy and strong with the plant that the present composition was handled, its attendant advantages is that disease or other effect of coercing are had higher resistance, and has the setting site of larger amt and higher output.
Describe in detail
The invention provides the method that a kind of usefulness contains the compositions-treated plant of effective active component on a kind of agricultural and a kind of reinforcement composition that contains the bacterial cultures that is selected from bacillus or soil bacteria, the consumption of described reinforcement composition be enough to improve use this reinforcements composition simultaneously, before or the effect of effective active component on the agricultural used afterwards.The effect of described raising gives the credit to the reinforcement composition that contains bacterium, this reinforcement composition can be used for reducing agricultural and goes up effective absorption of active ingredient, or when using described active component, improve the effect of effective active component on the employed agricultural by described bacillus with identical consumption.The described effect that has improved can be used for controlling with the restive weeds of conventional weed killer herbicide.
The reinforcement composition that contains bacterium
Described reinforcement composition contains brood cell, culture and the suspension of the bacterium that comes from bacillus or soil bacteria.Preferably, the bacterium that is used to strengthen composition is brood cell's form, because need suitably adjust temperature, pH, salinity.
Be applicable to that bacterium of the present invention comprises having and can improve the upward bacterium of the ability of the effect of effective active component of agricultural with any mechanism.By reading explanation and the example that this paper provided, those of ordinary skills can understand the method for the bacterial isolates of screening biologically active, and this bacterial strain can improve the ability of the effect of plant growth regulator, weed killer herbicide, interior suction fungicide or interior suction insecticide.For example, can under standard laboratory conditions, cultivate and keep candidate's bacterial isolates such as Bacillus cercus (Bacillus cereus).(for example, referring to Sambrook etc., " molecular cloning: laboratory manual ", publishing house of cold spring harbor laboratory (1989)).Can select special plant responsive such as compounds effective on the specific agricultural of weed killer herbicide or plant growth regulator or that have resistance.For example, as what proposed in the embodiment of this paper part, cotton can be used to estimate the biologically active of the bacterial isolates that makes up with plant growth regulator, the quantity in the present setting of its active body site and/or the cotton boll quantity that is produced, and compare with the similar plant with plant growth regulator or bacterial treatment only.Perhaps, can under in check condition, plant the plant of some fungal disease of the leaf rust of susceptible such as tomato leaf rot, potato spot disease, wheat stalk rust, corn smut or various plant species, handle with bacteria culture with a kind of suitable fungicide, fungicide and candidate's bacteria culture or list, described processing before with described pathogenic organisms inoculation plant, simultaneously or carry out afterwards, to estimate the ability that described bacterial classification improves the activity of described fungicide.The test of above type can under the condition of half control system, as carrying out in the greenhouse, perhaps be carried out under the condition of control relatively big Tanaka, as carrying out in the growth case.More than test relates to the routine screening that biology is carried out, and screens its invigoration effect to compound or composition on the known plants kind, and the parameter that is used to estimate its effect is known equally, and is to measure with conventional method.
The screening of described similar type can be used for the disclosed bacterial isolates of this paper, to produce strengthening effect to effective active component on the specific agricultural, be that Bacillus cercus (Bacillus cereus) bacterial strain of ATCC55675 provides and helps strong plain and herbicide atrazine to produce invigoration effect to plant growth regulator promptly by preserving number by the American type culture collection preservation, with screening to such as other agricultural of interior suction insecticide and interior suction fungicide upward effective active component have the bacteria culture of potential invigoration effect.
Particularly preferably be culture, brood cell and the suspension of soil bacteria, described soil bacteria be between the root of the vegetation type of in the soil of locality, vegetating and among the bacterium that occurs.With about 0.1 * 10
10-10 * 10
10The soil bacteria of the described locality of consumption foliage-spray of CFU/ acre, can enhancing relevant with breeding with plant growing natural mechanism makes it reach foot in making the active component on the described agricultural produce the degree of strengthening activity to the plant of handling.
The various bacteria bacterial classification of bacillus and known soil bacteria bacterial classification can be used for the present invention.Below be the catalogue that is used for bacterial classification of the present invention:
Bacterium from bacillus
Bacillus acidocaldarius (Bacillus acidocaldarius)
Sour soil bacillus (Bacillus acidoterrestris)
Alkaliphilic bacillus (Bacillus alcalophilus)
Honeycomb series bacillus (Bacillus alvei) bacillus pumilus (Bacillus aminoglucosidicus) bites amine bacillus (Bacillus aminovorans) bacillus amyloliquefaciens (Bacillus amyloliquefaciens) bacillus amylolyticus (Bacillus amylolyticus)
Bacillus anthracis (Bacillus anthracis) bacillus aneurinolytieus (Bacillus aneurinolyticus)
Honeybee bacillus (Bacillus apiarius) fixed nitrogen series bacillus (Bacillus azotofixans)
Bacillus brevis (Bacillus brevis)
Bacillus badius (Bacillus badius)
Ovum head-germ spore bacillus (Bacillus capitovalis)
Bacillus cercus (Bacillus cereus) Bacillus circulans (Bacillus circulans) feathering bacillus (Bacillus cirroflagellosus) the smelly bacillus of bacillus coagulans (Bacillus coagulans) colon (Bacillus colofoetidus) suberyl bacillus (Bacillus cycloheptanicus)
Plant table bacillus (Bacillus epiphytus) bacillus fastidiosus (Bacillus fastidiosus) filmanetous colony bacillus (Bacillus filicolonicus)
Bacillus firmus (Bacillus firmus) Fu Shi urinates bacillus (Bacillus freudenreidii)
Bacillus megaterium (Bacillus fructosus)
Bacillus subtilis (Bacillus globigii) bacillus globisporus (Bacillus globisporus)
Unusual bacillus (Bacillus insolitus) D-lactic acid bacillus (Bacillus laevolacticus)
Paenibacillus larvae (Bacillus larvae) bacillus laterosporus (Bacillus laterosporus)
Bacillus lautus (Bacillus lautus) delays disease bacilli (Bacillus lentimorbus)
Bacillus lentus (Bacillus lentus)
Bacillus licheniformis (Bacillus lechenigormis)
Series bacillus (Bacillus macquariensis) in bacillus macerans (Bacillus macerans) Ma Kuo
Morocco bacillus (Bacillus maroccanus)
Prolong bacillus (Bacillus macroides)
Bacillus cercus (Bacillus medusa)
Bacillus megaterium (Bacillus megaterium)
Bacillus mycoides (Bacillus mycoides)
Bafillus natto (Bacillus natto)
Bacillus subtilis (Bacillus nigrificans)
Feed bacillus (Bacillus pabuli)
Pacific Ocean bacillus (Bacillus pacificus)
Pantothenic acid bacillus (Bacillus pantothenticus)
Secondary bacillus brevis (Bacillus parabrevis)
Pasteur bacillus (Bacillus pasteurii)
Bacillus polymyxa (Bacillus polymyxa)
Japanese beetle bacillus (Bacillus popilliae)
Bacillus?pulvifa
Dust bacillus (Bacillus pulvifaciens)
Bacillus pumilus (Bacillus pumilus)
Spirit bacterium bacillus (Bacillus prodigiosus)
Have a liking for that cold bacillus (Bacillus psychrophilus) is cold separates sugared bacillus (Bacillus psychrosaccharolyticus) racemic lactic acid bacillus (Bacillus racemilacticus)
Bacillus sphaericus (Bacillus sphaericus) bacillus stearothermophilus (Bacillus stearothermophilus)
The hot denitrification bacillus of bacillus subtilis (Bacillus subtilis) (Bacillus thermodenitrificans) bacillus aneurinolytieus (Bacillus thiaminolyticus)
Bacillus thuringiensis (Bacillus thuringiensis)
Bacillus subtilis (Bacillus uniflagellatus)
Strong bacillus (Bacillus validus)
Soil bacteria
Band epidemic disease achromobacter (Achromobacter pestifer)
Alcaligenes eutrophus (Alcaligenes eutrophus)
Alcaligenes latus (Alcaligenes latus)
Autotrophy does not have branch sour bacterium (Amycolata autotrophica)
The former capsule bacterium of transition (Archangium gephyra)
Sticking arthrobacterium (Arthrobacter viscosus)
Blown-ball Azotobacter (Azotobacter chroococcum)
Bacillus?acidovorans
Bacillus brevis (Bacillus brevis)
Bacillus cercus (Bacillus cereus)
Bacillus circulans (Bacillus circulans)
Unusual bacillus (Bacillus insolitus)
Bacillus laterosporus (Bacillus laterosporus)
Bacillus megaterium (Bacillus megaterium)
Mocha husband bacillus (Bacillus mojavensis)
Deep shape bacillus (Bacillus mycoides)
Pasteur bacillus (Bacillus pasteurii)
Bacillus polymyxa (Bacillus polymyxa) is cold separates sugared bacillus (Bacillus psychrosaccharolyticus)
Bacillus sphaericus (Bacillus sphaericus)
Bacillus subtilis (Bacillus subtilis)
Thickness bacillus (Bacillus viscosus)
The black blue Salmonella (Chainia hygroatrocyanea) of admiring of suction
Clostridium absonum (Clostridium absonum)
Dark brown sporangiocyst bacillus (Cystobacter fuscus)
Yue Shi Cytophaga (Cytophaga johnsonae)
Adhesion sword bacterium (Ensifer adhaerens)
Quick raw silk germ (Hyphomicrobium facilis)
The bronze micromonospora of micrococcus luteus (Microccoccus luteus) (Micromonospora chalcea) ultramarine micromonospora (Micromonospora coerulea)
The many curved micromonosporas (Micromonospora polytrota) of grey micromonospora (Micromonospora grisea)
Microteraspora?glauca
Field mycobacterium (Mycobacterium agri)
Like to know mycobacterium (Mycobacterium aichiense)
Golden mycobacterium (Mycobacterium aurum)
Thousand field mycobacteriums (Mycobacterium chitae)
Chu cloth mycobacterium (Mycobacterium chubuense)
Di Shi mycobacterium (Mycobacterium diernhoferi)
Mycobacterium fortutitum (Mycobacterium fortuitum)
The secondary mycobacterium fortuitum of new golden mycobacterium (Mycobacterium neoaurum) (Mycobacterium parafortuitum)
Mycobacterium terrae (Mycobacterium terrae) heat resistanceheat resistant mycobacterium (Mycobacterium thermoresistibile)
Myxococcus coralloides (Myxococcus coralloides)
Myxococcus fulvus (Myxococcus fulvus)
Myxococcus macrosporus (Myxococcus macrosporus)
Yellow myxobacter (Myxococcus xanthus)
Nannocystis exedens (Nannocystis exedens) nitrosolobus multiformis (Nitrosolobus multiformis)
Brazil's Nocard's bacillus (Nocardia brasiliensis)
White group Nocard's bacillus (Nocardioides albus)
Human pallid bacillus (Ochrobactrum anthropi)
Many cloth of fiber bacterium (Polyangium cellulosum) pseudomonas aeruginosa (Pseudomonas aeruginosa) pseudomonas fluorescens (Pseudomonas fluorescens)
Pseudomonas glathei (Pseudomonas glathei)
The aquatic green sugared sporangium of the pointed saccharobacillus of engler bacterium (Rahnella aquatilis) (Saccharobacterium acuminatum) (Saccharomonospora viridis) that draws
Serratia marcescens (Serratia marcescens)
A Nandeshi streptomycete (Streptomyces anandii)
Streptomyces aureofaciens (Streptomyces aureofaciens)
Streptomyces chartreusis (Strptomyces chartreusis)
Dark blue streptomycete (Strptomyces cyaneus)
Strptomyces cymarogenes streptomyces diastatochromogenes (Strptomyces diastatochromogenes)
Yellow grey streptomycete (Strptomyces flavogriseus)
Glue sample streptomycete (Strptomyces gelaticus)
Streptomyces hygroscopicus (Strptomyces hygroscopecus)
Indigo streptomycete (Strptomyces indigocolor)
Block special slide fastener mould (Strptomyces katrae)
Li Boman streptomycete (Strptomyces lipmanii)
Long spore streptomycete (Strptomyces longisporus)
Heap spore streptomycete (Strptomyces massasporeus)
Noble streptomycete (Strptomyces nobilis)
Soil flavor streptomycete (Strptomyces odorifer)
Big palace streptomycete (Strptomyces omiyaensis)
Small streptomycete (Strptomyces parvulus)
Abortion within the first month of pregnancy look streptomycete (Strptomyces phaeochromogenes)
False light gray streptomycete (Strptomyces pseudogriseolus)
Rose yellow streptomycete (Strptomyces roseoflavus) rust red wax yellow streptomycete (Strptomyces rubiginosohelvolus)
Ground, Shandong streptomycete (Strptomyces rutgersensis) sclerotium pellet chain mould (Strptomyces sclerogranulatus)
Poison three plain streptomycetes (Strptomyces toxytricini)
Streptomyces violaceoruber (Strptomyces violaceoruber)
Streptomyces violaceus (Strptomyces violaceus)
Slightly purple streptomycete (Strptomyces violarius) thermoactinomyces vulgaris (Thermoactinomyces vulgaris)
Thiobacillus denitrificans (Thiobacillus denitrificans)
The no pseudomonas fluorescens of thiobacillus thioparus (Thiobacillus thioparus) (non-fluorescent pseudomonas)
Rhizobium (Rhizobium)
Agrobacterium (Agrobacterium)
Produce urea rod bacillus (Corynebacterium ureafaciens)
Produce urea arthrobacterium (Arthrobacter ureafaciens)
Pseudomonas aeruginosa (Pseudomonas aeruginosa)
Bacillus fastidiosus (Bacillus fastidosus)
Micrococcus denitrificans (Micrococcus dentrificans)
Mycobacterium graminis (Mycobacterium phlei)
Aerobacter aerogenes (Aerobacter aerogenes)
String ball sickle spore (Fusarium moniliforme)
Capsule tissue spore slurry bacterium (Histoplasma capsulata)
Penicillium chrysogenum (Penicillinum chrysogenum)
Useful especially is bacillus subtilis, Bacillus cercus and bacillus megaterium.Bacillus subtilis and Bacillus cercus are naturally occurring in the world soil saprophytic bacterias.Enumerated 182 kinds of different bacillus subtilis strains in the American Type Culture Collection of version in 1992, this data is made the list of references of this paper by receipts.Below be to can be used for bacillus subtilis catalogue of the present invention:
Bacillus subtilis (B.Subtilis)
ATCC?10783
ATCC?15818
ATCC?15819
ATCC 15245 bafillus nattos (Bacillus natto)
ATCC 15134 bacillus subtilises (Bacillus uniflagellatus)
ATCC?13542
ATCC?13472
ATCC?15575
ATCC?27505
ATCC 21697 splits nitrile achromobacter (Achromobacter nitriloclastes)
ATCC?15811
ATCC 4925 ( Bacillus nigrificans ) ATCC 27370ATCC 6051aATCC 7003ATCC 11838ATCC 15563ATCC 33234ATCC 25369ATCC 27689ATCC 55033ATCC 13933ATCC 15244ATCC 27328ATCC 12695ATCC 12100ATCC 21554ATCC 21555ATCC 15561ATCC 15562ATCC 9799ATCC 12711ATCC14593ATCC4944ATCC31002ATCC31004ATCC9943ATCC13407ATCC7067ATCC29056ATCC31524ATCC31526ATCC21359ATCC21360ATCC13954ATCC13955ATCC15044ATCC33677ATCC31003ATCC31522ATCC465N.ATCC12432ATCC43223ATCC13952ATCC13953ATCC14662ATCC15039ATCC15040ATCC15041ATCC15042ATCC15043ATCC15181ATCC15182ATCC15183ATCC15184ATCC21183ATCC21336ATCC49343ATCC6537ATCC21394ATCC8473ATCC31523ATCC31525ATCC31527ATCC29233ATCC14660
ATCC14661
ATCC31268
ATCC4925
ATCC55405
ATCC9524
ATCC15476
ATCC23858
ATCC23859
ATCC7060
ATCC7058
ATCC7059
ATCC7480(Bacillus?endoparasiticus)
ATCC21584
ATCC31022
ATCC21331
ATCC21332
ATCC21777
ATCC21778ATCC6598 bacillus licheniformis (Bacillus licheniformis)
ATCC49822
ATCC23857
ATCC19221
ATCC9858
ATCC21742
ATCC4529
ATCC35148
ATCC33608
ATCC19549
ATCC19550
ATCC21556
ATCC31340
ATCC49760ATCC53325ATCC14807ATCC21228ATCC15512ATCC15841ATCC10774ATCC31091ATCC31092ATCC31094ATCC31096ATCC31097ATCC35946ATCC39374ATCC11774ATCC15116ATCC35021ATCC31954ATCC19062ATCC23059ATCC53115ATCC15115ATCC13956ATCC21952ATCC82ATCC21603ATCC31785ATCC21697ATCC15477ATCC31098ATCC19162ATCC14617ATCC14618
ATCC33713
ATCC33714
ATCC55422
ATCC6461
ATCC21007
ATCC21770
ATCC6984
ATCC19163
ATCC21663
ATCC19217
ATCC19219
ATCC19220
ATCC21005
ATCC21006
Be used for preferred bacillus subtilis strain of the present invention and comprise GB03.In the past, bacillus subtilis strain GB03 was considered to biological fungicide, and by commercialization as seed treatment, this inorganic agent by Gustafson company (Plano, Texas 75093) with KODIAK
TMHB or GUS2000
TMFor trade mark is sold (EPA number of registration 7501146).This product is to sell with 2.75% powderous preparations form, and each restrains to contain in this powderous preparations and is no less than 5.5 * 10
10Individual active brood cell, and use with the consumption of per 100 pounds of seed 2-4 ounces.Its guide for use shows that this product only can be used to handle crop seed.It is said that this bacterial strain can be settled in the root system of growth, and compete with the pathogenic organisms that might encroach on root system.Use on not mentioned blade face.
Below be to can be used for Bacillus cercus catalogue of the present invention:
Bacillus cercus (B.cereus)
ATCC55675(BP01)
ATCC13824 NCIB2600 Bacillus cercus fluorescence mutation (Bacillus cereus
var.fluorescens)
ATCC14603
ATCC15816
ATCC15817
ATCC13472
ATCC14737
ATCC9592(Bacillus?metiens)
ATCC27877
ATCC19637
ATCC11950
ATCC23261
ATCC6464
ATCC11773 deceives rot Erwinia (Erwinia atroseptica)
ATCC10876
ATCC10987
The ATCC4342 newborn bacillus (Bacillus lacticola) that dwells
ATCC21182
The milky white bacillus (Bacillus albolactis) of ATCC7004
ATCC10702
ATCC12480
ATCC49063
ATCC2 field bacillus (Bacillus agri) ATCC19265 Bacillus cercus A Lai subspecies (Bacillus cereus subsp.
alesti)
ATCC21634
ATCC12826
ATCC21768
ATCC21769
ATCC21771
ATCC21772
ATCC9139
ATCC21928
ATCC27522
ATCC31430
ATCC43881
ATCC246 bacillus lentimorbus (Bacillus lactimorbus)
ATCC21770 Bacillus cercus (Bacillus cereus) ATCC21929 Bacillus cercus (Bacillus cereus)
ATCC13367
ATCC31429
ATCC31293 ATCC21366 bacillus coagulans (Bacillus coagulans) ATCC25621 Bacillus cercus (Bacillus medusa)
ATCC7039 (Bacillus metiens) ATCC14893 bacillus subtilis (Bacillus subitilis) ATCC9818 breast bacillus (Bacillus lactis)
ATCC33018
ATCC33019
ATCC55055
ATCC53522
ATCC13366
ATCC7064(Bacillus?siamensis)
ATCC11949 ATCC10792 Bacillus cercus (Bacillus cereus)
ATCC27348
ATCC23260(Bacillus?endorhythmos)
ATCC13061ATCC13062 bacillus megaterium (Bacillus megaterium)
ATCC25972
ATCC14597
ATCC19646
ATCC49064
ATCC11778
ATCC39152
ATCC19146FDA pseudomonad (Pseudomonas sp.) bacterial strain PCI818
Be used for preferred bacillus cereus strain of the present invention and comprise strains A TCC No.55675.In another embodiment, be used for the characteristic that bacillus cereus strain of the present invention has ATCC No.55675.In another embodiment, be used for the characteristic that bacillus subtilis strain of the present invention has ATCCNo.55675.Below be the catalogue that can be used for the bacillus megaterium among the present invention:
Bacillus megaterium (B.Megaterium)
ATCC33166
ATCC33167
ATCC33168
ATCC33169
ATCC12872
ATCC33164
ATCC11478
ATCC33165
ATCC11561
ATCC11561a
ATCC11561b
ATCC11561c
ATCC11561d
ATCC11561e
ATCC6458
ATCC6459
ATCC14946
ATCC27327
ATCC33729
ATCC9885
ATCC13639
ATCC13632
ATCC15374
ATCC49099
ATCC49096
ATCC43725
ATCC19136
ATCC25848
ATCC4531ATCC13402ATCC15046ATCC15047ATCC25300ATCC19380ATCC13368ATCC21181ATCC35985ATCC39383ATCC11562ATCC15127ATCC15128ATCC7703ATCC14945ATCC15177ATCC19218ATCC25833ATCC15450ATCC19135ATCC19137ATCC21916ATCC8245ATCC15781ATCC31294ATCC15117ATCC15118ATCC19160ATCC19161ATCC89ATCC21209ATCC35076ATCC49098ATCC39118ATCC7051ATCC7052ATCC10778ATCC8011ATCC7056ATCC14581ATCC13062ATCC49097ATCC19213ATCC71ATCC35075ATCC21603ATCC21738ATCC72ATCC21737ATCC49095ATCC15451
Active component
Being used on the agricultural of the present invention effective active component can select from multiple material, and these materials can exert an influence to the metabolism of the plant that is subject to processing and by its metabolism.For pre-mixed material, need to select active component, selected composition non-degradable or under the condition of the reinforcement composition that contains bacterium that is used to keep brood cell's form long term storage can not suffer damage.
Generic noun " plant growth regulator " comprises the multiple active component that influences plant by different way.In general, plant growth regulator comprises plant growth inhibitor, plant growth promoter, and weed killer herbicide.
Be applicable to that plant growth promoter of the present invention comprises auxin, at least a as in 84 kinds of gibberellin of having identified, preferred GA
3, GA
4, GA
5, GA
7And GA
9Cytokinin (for example, zeatin, the basic element of cell division, benzyladenine, dihydro zeatin and isopentennyladenine); Auximone (for example, heteroauxin (IAA), indolebutyric acid (IBA), and methyl (NAA)); ONP sodium; Right-p-nitrophenol sodium; 5-nitro-healing wood phenol sodium; With 2,4, the multi-hydroxy carboxy acid of 5 and 6 carbon structures; Ethrel; With multiple nitrogenous or phosphorous fertilizer.
Be applicable to that plant growth inhibitor of the present invention comprises Chlormequat, help strong plain and maleic acid hydrazide and ester thereof.Above-mentioned plant growth regulator can influence and change the metabolic process of plant, to promote or the inhibition plant growing.Above-mentioned all conditioning agents all can use according to consumption that is indicated on Product labelling by the manufacturer and number of times.
Weed killer herbicide comprises triazine (for example, atrazine), urea, glyphosate, sulfosate, glyfosinate, and sethoxydim.
The suitable systemics of plant absorbing, transportation and the assimilation process that has had benefited from strengthening comprises systemic insecticide and interior suction fungicide.Have benefited from plant systemics of the present invention particularly including, insecticide aldicarb, orthene, furadan, Rogor, thimet, and Terbufos.
The interior suction fungicide that has benefited from mixture of the present invention comprises tridemorph, metalaxyl, the dichlorophenyl first and second basic dioxy imidazolidine carboxylic acid amides, fosetyl-aluminum, topsin, benomyl, triadimeson, carboxin, oxycarboxin, carbendazim, thiabendazole, topsin, Milstem, the phonetic bacterium spirit of sulphur, and Milcurb.
Can in fact comprise with the plant that the present invention handles be grown in the soil, cutting plant of being subjected to that agricultural goes up that effective active component influences.Typical plant comprises commodity food crop (for example, corn, wheat and soybean), and Chinese sorghum needs and unwanted herbage weeds, medicinal herbs etc.
The present invention is suitable for improving the fruit yield of plant, and described plant can produce the setting site, grows up to fruit by these setting sites.Described plant optimization comprises any of thick agricultural commodity grain, particularly cotton, soybean, peanut, grape, apple, oranges and tangerines (for example, lemon, bitter orange, orange, grape), berry (for example, strawberry, blackberry, raspberry), stem tuber (for example, potato, sweet potato), corn, cereal is (for example, wheat, rice, rye), tomato, onion, melon (for example, watermelon, cucumber and cantaloupe).
Using method
Composition of the present invention can adopt the various physical form of liquid, emulsion, suspension, solid particle, aggregate or composite particles (for example, the active component solid that is carried by a kind of inert carrier particle).Generally, by routine techniques the composition of each physical form is executed on plant leaf blade.
The Gram-positive bacteria bacterial strain can be used for described active component with cell, brood cell, culture or its form of suspension, in liquid or dispersible solid form, described reinforcement composition adds in the fluid dispenser container of spray tank or other form with stable moisture concentrated solution form, brood cell's farming degree of described concentrated solution is equivalent to about 300,000 colony-forming units/milliliter (CFU/ml) is to about 1.5 hundred ten thousand CFU/ml, 1-1.2 1,000,000 CFU/ml preferably approximately, making its consumption of composition that sprays to plant leaf blade is about 0.1 * 10
10-100 * 10
10The CFU/ acre is preferably about 0.1 * 10
10-10 * 10
10The CFU/ acre most preferably is about 0.5 * 10
10CFU/ acre (0.5fl.oz./acre concentrate) is to about 8 * 10
10CFU/ acre (2fl.oz./acre concentrate).In preferred embodiments, can also optionally contain effective active component on the described agricultural in the described spray tank, so that spray two kinds of compositions simultaneously.
Before sowing, can carry out dry mixed to the composition of solid form or make aggregate.One or more conventional adjuvants can be used to strengthen dispersiveness, degraded, to adhering to of blade etc.
According to the method difference that described solution is applied to plant surface, its concrete consumption can change to some extent.For example, the dilution ratio and the consumption that adopt of aerial spraying is different from wide cut spraying, manual sprayer or broadcasts sowing particle.Conventional equipment can be used for spraying.If desired, described reinforcement composition can be mixed with other inorganic agent, and simultaneously or with one independently treatment step spray.Foliage-spray is the method for optimizing that is used to improve the setting site quantity of fruit-bearing plant.
Also described concentrate can be used to prepare mixture that can use immediately, packaged.After such preparation, described reinforcement composition is diluted in this packing container about 150,000-600, the amount of 000CFU/ml, and employed condition is suitable for guaranteeing that the bacterium composition keeps its brood cell's form, but after using, can breed.
For a lot of bacteriums, use to be lower than 7 pH (being acid pH) and can to keep this bacterium to be in brood cell's form.If necessary, any common acidulant or buffer solution (preferred food grade or thought the reagent of " it is safe generally being considered as " by Environmental Protection Agency) can be used to the acid pH that keeps suitable, with the stability of guaranteeing to store.Under described acid condition, these brood cells keep stable, and show excellent storage stability.After diluting use and using, the pH of this solution can be elevated to and surpass 7, thereby causes the bacterium in the described reinforcement composition to form bacterium colony alive, genitality.Therefore, these bacteriums can breed on the plant surface of handling, and promote agricultural to go up the transfer of effective active component.
The described reinforcement composition that contains bacterium can be used as independently inorganic agent use, or goes up effective active component with multiple other agricultural and use simultaneously.Effective active component comprises plant growth promoter on the useful agricultural, plant growth inhibitor, weed killer herbicide, interior suction insecticide and interior suction fungicide.Preferably, described composition is the combination of the reinforcement composition of plant growth inhibitor or weed killer herbicide and the bacterial strain that contains pure substantially bacillus subtilis, Bacillus cercus or ATCC55675 (BP01), and the consumption of said composition is at least 0.1 * 10
10The CFU/ acre.
In a kind of particularly preferred embodiment, a kind of no gibberellin that contains described bacillus is strengthened composition be applied on the blade of cotton plants.Meanwhile, with helping strong plain this plant of handling.Containing the described reinforcement composition that contains bacterium (preferred ATCC55675) and helping the water tank mixture of strong element is the convenient method that uses above two kinds of compositions simultaneously.If carry out premixed, can under the pH of about 4-6.5, preserve the described reinforcement composition that helps strong element and contain bacterium, most preferably be under the pH of about 5-6.5 and preserve.
Should be pointed out that preparation of the present invention does not preferably comprise the combination that can act on the material of plant metabolism in conflicting mode.For example, normally will help strong element to be used on the cotton leaf, to suppress auxin and to suppress nourishing and growing of this plant.Preferably do not contain auxin in the prepared preparation,, help strong plain effectiveness thereby reduce, and cause inconsistent result because help strong element and this somatotropin to produce inconsistent effect to plant metabolism.But, by the present invention, help the strong plain consistent processing plant that has high yield, growth is more healthy and stronger and disease is had higher resistance that produces with the combination meeting of ATCC55675.
Although do not wish to be subjected to the restriction of any specific theory of operation, with regard to the reinforcement composition that contains bacterium with help with regard to strong plain being used in combination on cotton, as if this bacterium can influence the growth mechanism of plant, increasing the cotton boll maintenance dose on the first and second setting sites, and by on common vegetative shoot, producing and keeping the setting site to increase the cotton boll sum.
For the purpose of the present invention, helping strong element is to use with usage amount, and is to use in the conventional stage of cotton plants growth.Help strong plain conventional amount used up to about 60 gram/acres (25 gram/acre) or about 1-16 ounces/acre, consumption each time is about 2.5 gram/acres (1.0 gram/hectares), the percentage by weight that promptly uses 2 ounces/acre is 4.2% solution, to 10 gram/acres (4.1 gram/hectare), the identical percentage by weight that promptly uses 8 ounces/acre is 4.2% solution.If the employed strong plain purity and/or active higher or lower that helps, its concrete consumption should help strong plain active variation to raise or reduce according to commonly used.
Embodiment
Example 1
With 1: 1 volume ratio prepare percentage by weight be 4.2% help strong plain and 560,000CFU/ml Bacillus cercus (the sample BP01 of laboratory, aqueous mixture ATCC55675).With conventional sprayer by on the blade of 4 sprayings with the cotton plants in the experimental plot among the experimental plot of described spray solution in the A of land for growing field crops (in 50 acres 10 acres, Mississippi) and the land for growing field crops B (in 60 acres 8 acres, Tennessee).Liquid consumption with 4 ounces every acre carries out the processing of a dosage to land for growing field crops A, handles with the consumption of every acre 8 fluid ounce in 5 weeks afterwards.
The processing scheme difference of land for growing field crops B.3 consumptions of land for growing field crops B are that every acre 4 fluid ounce (was used 24 hours at interval for the first time and for the second time.Used for the second time and for the third time 6 days at interval).Last use among the B of land for growing field crops is after 11 days, and its consumption is every acre 8 fluid ounce.Under all occasions, the contrast land for growing field crops all is only with strong plain processing of helping of same amount.
After handling, from land for growing field crops A and B, to take a sample, sampling is carried out in representative plant with hand.All known deviations are all brought in the contrast, and this contrast is only with helping strong plain the processing.
In land for growing field crops A and B, measure initial plant height and the final plant height of handling cotton plants.Total plant height in described contrast land for growing field crops is suitable with the plant height of handling with the present invention.Referring to table 1.
Table 1
| The land for growing field crops | Starting altitude (inch) | Final height (inch) |
| The A-contrast | ????293 | ????601 |
| A-handles | ????291 | ????597 |
| The B-contrast | ????338 | ????580 |
| B-handles | ????334 | ????636 |
In vegetative period, adjoining tree and the difference of handling between the plant are found easily.When shoot only contains the square of first month of setting, the extra setting branch of unusual a large amount of and volume can appear, and at this moment, seat of honour fruit branch leaves stem.A lot of setting branches all with seat of honour fruit branch equally greatly or almost same big, and have fruit prior to first flower.Extra fruit also appears at the junction of setting branch and stem.
Along with flower extends to plant top, and only discovery cotton boll or deletion segment below these flowers, the background setting of amount beyond expectation also can appear.In square, flower, little cotton boll and " Kai Fang cotton boll " (rare), observed the two-seater fruit.Although two-seater fruit phenomenon is in contrast and handle all appearance to some extent in the plant, the ratio that occurs in handling plant is higher.
About 3-4 week before finishing vegetative period, the cotton boll number on the setting number of sites of inspection plant, first segment and second joint and the type of cotton boll.Testing result is as shown in table 2.
Table 2
| The land for growing field crops | The site | Cotton boll | ??％ | Open cotton boll | Open cotton boll+blue or green cotton boll | Other cotton boll |
| A-contrast A handles | ??240 ??292 | ??115 ??155 | ??48 ??53 | ??73 ??173 | ????270 ????359 | ???25 ???103 |
| The B-contrast | ??344 | ??207 | ??60 | -- | -- | ??81 |
| B-handles | ??366 | ??234 | ??64 | -- | -- | ??163 |
In the B of land for growing field crops, when experimental period finishes, 20 plant are checked.Described plant is cut out.In control group, do not have open cotton boll, the cotton boll of 7 openings is only arranged in processed group.But, compare with adjoining tree, the more immature fruit of handling on the plant is bigger, also more ripe.This shows that the plant of handling is with faster and more favourable speed growth.The plant of handling has the cotton boll that adds up 265, and corresponding therewith, control group only has 238 cotton bolls, and its recruitment is 11%.
The setting quantity of the plant of handling on vegetative shoot also increases to some extent, and its ratio is 88: 51, and recruitment is approximately 73%.The plant of handling (promptly present fruit branch position 3 and fruit in larger scope) aspect other setting also increases to some extent, and its ratio is 86: 50 (recruitment is 72%).Estimated weight (unginned cotton) from the fruits of 20 plant is also higher in handling plant, handles and the weight of contrast is respectively 1796 grams and 1456 and restrains (recruitment is 23%).
Extra fruit on the described plant can produce such imagination: promptly extra plant part and immature fruit can consume this plant system.Cotton boll can be opened faster, but can have a negative impact to output.This imagination also is unrealized.Handling plant can be with comparison according to faster speed and more healthy state growth.
In order to reduce error, all cottons all are to gather from testing big Tanaka with hand, comprise the unnapped cotton of gathering from the open cotton boll of part.Described unnapped cotton is usually from the cotton boll that can correctly not open, be positioned at the plant bottom forever or from the cotton boll of the firm opening that is positioned at this plant top.Unginned cotton weight and the blue or green cotton boll quantity handling the land for growing field crops and contrast per 10 a feet long cotton ridge of big Tanaka have been provided in the table 3.
Table 3
| The land for growing field crops | Cotton seeds (gram) | The quantity of blue or green cotton boll |
| The A-contrast | ????5322 | ????97 |
| A-handles | ????6287 | ????0 |
| The B-contrast | ????4532 | ????175 |
| B-handles | ????5058 | ????42 |
Result of the test shows, according to the present invention, helps strong element and the reinforcement composition that contains ATCC55675 to be used in combination simultaneously, can help higher output of cotton of strong plain generation and more healthy and stronger plant than only using.
Example 2
Bacillus cereus strain BP01 (ATCC55675) is used in combination with widely used herbicide atrazine, whether can the control that be acknowledged as the weeds that are difficult to kill with triazine be exerted an influence to measure BP01.Use atrazine that corn, Chinese sorghum and other are done the deposits yields control in one season, the usage amount of its suggestion is every acre of 2 pounds of active components.Under sufficiently high consumption, has non-selective weeds control ability as finding atrazine under the consumption of present embodiment.
In sand soil, each test is repeated 3 times, the sub-district area is 12 feet * 25 feet, adopts complete closed form immediately.Weeds highly are 2-6 inch (5-15cm) when handling.Use atrazine with aqueous solution form, its consumption is equivalent to every acre of 1 or 2 pound of active component.With the BP01 concentrate to be equivalent to every acre 0.5 or 1 fluid ounce (0.5-1 * 10
10The CFU/ acre) consumption adds in the described atrazine.In order to compare, in a usual manner crop oil concentrate (about 85% paraffin oil and about 17% surfactant) and triazine herbicides are used in combination, to improve its effectiveness.Provided control effect among the table 4-8 to Florida kitchen garden (table 4), bull grass (table 5), Bermuda grass (table 6), dog fennel (table 7) and primrose (table 8).
Table 4-Florida kitchen garden
| Handle | Control percentage after handling | |||
| 4 days | 9 days | 16 days | 23 days | |
| Contrast | ????0 | ????0 | ????0 | ????0 |
| Atrazine (1 1b.) | ????3 | ????33 | ????42 | ????45 |
| Atrazine (2 1b.) | ????17 | ????57 | ????72 | ????83 |
| Atrazine (1 1b.)+crop oil concentrate (1 gallon) | ????28 | ????62 | ????68 | ????68 |
| Atrazine (1 1b.)+0.5oz.BP01 | ????27 | ????43 | ????60 | ????60 |
| Atrazine (1 1b.)+1oz.BP01 | ????22 | ????53 | ????65 | ????67 |
Table 5-bull grass
| Handle | Control percentage after handling | |||
| 4 days | 9 days | 16 days | 23 days | |
| Contrast | ????0 | ????0 | ????0 | ????0 |
| Atrazine (1 1b.) | ????10 | ????40 | ????40 | ????55 |
| Atrazine (2 1b.) | ????25 | ????70 | ????80 | ????90 |
| Atrazine (1 1b.)+crop oil concentrate (1 gallon) | ????25 | ????40 | ????65 | ????60 |
| Atrazine (1 1b.)+0.5oz.BP01 | ????35 | ????40 | ????55 | ????63 |
| Atrazine (1 1b.)+1oz.BP01 | ????30 | ????60 | ????80 | ????90 |
Table 6-Bermuda grass
| Handle | Control percentage after handling | |||
| 4 days | 9 days | 16 days | 23 days | |
| Contrast | ????0 | ????0 | ????0 | ????0 |
| Atrazine (1 1b.) | ????0 | ????10 | ????15 | ????15 |
| Atrazine (2 1b.) | ????8 | ????15 | ????22 | ????27 |
| Atrazine (1 1b.)+crop oil concentrate (1 gallon) | ????25 | ????27 | ????30 | ????43 |
| Atrazine (1 1b.)+0.5oz.BP01 | ????7 | ????20 | ????37 | ????68 |
| Atrazine (1 1b.)+1oz.BP01 | ????10 | ????23 | ????53 | ????63 |
Table 7-dog fennel
| Handle | Control percentage after handling | |||
| 4 days | 9 days | 16 days | 23 days | |
| Contrast | ????0 | ????0 | ????0 | ????0 |
| Atrazine (1 1b.) | ????15 | ????40 | ????55 | ????60 |
| Atrazine (2 1b.) | ????17 | ????55 | ????70 | ????95 |
| Atrazine (1 1b.)+crop oil concentrate (1 gallon) | ????25 | ????75 | ????93 | ????98 |
| Atrazine (1 1b.)+0.5oz.BP01 | ????33 | ????88 | ????96 | ????99 |
| Atrazine (1 1b.)+1oz.BP01 | ????30 | ????90 | ????97 | ????100 |
Table 8-primrose
| Handle | Control percentage after handling | |||
| 4 days | 9 days | 16 days | 23 days | |
| Contrast | ????0 | ????0 | ????0 | ????0 |
| Atrazine (1 1b.) | ????10 | ????40 | ????50 | ????60 |
| Atrazine (2 1b.) | ????30 | ????65 | ????70 | ????80 |
| Atrazine (1 1b.)+crop oil concentrate (1 gallon) | ????20 | ????83 | ????88 | ????93 |
| Atrazine (1 1b.)+0.5oz.BP01 | ????20 | ????60 | ????72 | ????88 |
| Atrazine (1 1b.)+1oz.BP01 | ????23 | ????70 | ????65 | ????80 |
As can be seen, BP01 generally can improve the effect of atrazine in 23 days after handling from table 4-8.For Bermuda grass, dog fennel and primrose, use 1 pound atrazine and bacillus ratio to use 2 pounds atrazine to have better control effect, and these two kinds of processing have identical control effect to the bull grass.The phenomenon that control effect with 1 pound/acre atrazine and BP01 reduces than the control effect of using 2 pounds of/acre atrazines to handle has only appearred on the kitchen garden of Florida.
Similarly, in all weeds except Florida kitchen garden and primrose, compare with the mixture of crop oil concentrate with atrazine, BP01 can improve the control effect of atrazine.This raising shows that this bacillus is not to play a part surfactant, but metabolic activity or transfer characteristic by improving the reagent that uses simultaneously or reach these two kinds of purposes simultaneously and improve its effect.
More than Shuo Ming purpose only is that the present invention will be described, it should be considered as the qualification to the claims scope.
Claims (67)
1. one kind is used to improve the composition that agricultural goes up the activity of effective active component, and comprising: (a) effective active component on a kind of agricultural, this active component are plant growth regulator, weed killer herbicide, interior suction fungicide or interior suction insecticide; (b) a kind of suitable bacillus or brood cell, culture or the suspension of soil bacteria are strengthened composition, and its prerequisite is that when described plant growth regulator was a kind of plant growth inhibitor, said composition did not contain auxin.
2. composition as claimed in claim 1, wherein, described plant growth regulator comprises helping strengthens element.
3. composition as claimed in claim 1, wherein, described plant growth regulator comprises Chlormequat.
4. composition as claimed in claim 1, wherein, effective active component comprises weed killer herbicide on the described agricultural.
5. composition as claimed in claim 4, wherein, described weed killer herbicide is a triazine.
6. composition as claimed in claim 4, wherein, described weed killer herbicide is glyphosate or sulfosage.
7. composition as claimed in claim 1 wherein, is inhaled fungicide in effective active component comprises on the described agricultural.
8. composition as claimed in claim 1, wherein, effective active component comprises plant growth regulator on the described agricultural.
9. composition as claimed in claim 1, wherein, described plant growth regulator is a plant growth inhibitor.
10. composition as claimed in claim 1 wherein, is inhaled insecticide in effective active component comprises on the described agricultural.
11. composition as claimed in claim 1, wherein, described reinforcement composition contains a kind of bacterial isolates from bacillus.
12. composition as claimed in claim 1, wherein, described reinforcement composition contains a kind of soil bacteria bacterial strain.
13. composition as claimed in claim 1, wherein, described reinforcement composition contains a kind of Bacillus cercus.
14. composition as claimed in claim 1, wherein, described reinforcement composition contains the Bacillus cercus of the feature of a kind of ATCC55675 of having.
15. composition as claimed in claim 1, wherein, described reinforcement composition contains a kind of bacillus subtilis.
16. composition as claimed in claim 1, wherein, described reinforcement composition contains the bacillus subtilis of the feature of a kind of ATCC55675 of having.
17. composition as claimed in claim 1, wherein, described reinforcement composition contains a kind of bacillus megaterium.
18. composition as claimed in claim 1, wherein, described reinforcement composition contains ATCC55675.
19. an on-liquid composition comprises: (a) effective active component on a kind of agricultural, this active component are plant growth regulator, weed killer herbicide, interior suction fungicide or interior suction insecticide; (b) a kind of suitable bacillus or brood cell or the culture of soil bacteria are strengthened composition, and its prerequisite is that when described plant growth regulator was a kind of plant growth inhibitor, said composition did not contain auxin.
20. as the composition of claim 19, wherein, effective active component comprises plant growth regulator on the described agricultural.
21. as the composition of claim 19, wherein, described plant growth regulator is a plant growth inhibitor.
22. as the composition of claim 19, wherein, described plant growth regulator comprises helping strengthens element.
23. as the composition of claim 19, wherein, described plant growth regulator comprises Chlormequat.
24. as the composition of claim 19, wherein, effective active component comprises weed killer herbicide on the described agricultural.
25. as the composition of claim 24, wherein, described weed killer herbicide is a triazine.
26. as the composition of claim 24, wherein, described weed killer herbicide is glyphosate or sulfosage.
27., wherein, inhale fungicide in effective active component comprises on the described agricultural as the composition of claim 19.
28., wherein, inhale insecticide in effective active component comprises on the described agricultural as the composition of claim 19.
29. as the composition of claim 19, wherein, described reinforcement composition contains a kind of bacterial isolates from bacillus.
30. as the composition of claim 19, wherein, described reinforcement composition contains a kind of soil bacteria bacterial strain.
31. as the composition of claim 19, wherein, described reinforcement composition contains a kind of Bacillus cercus.
32. as the composition of claim 19, wherein, described reinforcement composition contains the Bacillus cercus of the feature of a kind of ATCC55675 of having.
33. as the composition of claim 19, wherein, described reinforcement composition contains a kind of bacillus subtilis.
34. as the composition of claim 19, wherein, described reinforcement composition contains the bacillus subtilis of the feature of a kind of ATCC55675 of having.
35. as the composition of claim 19, wherein, described reinforcement composition contains a kind of bacillus megaterium.
36. as the composition of claim 19, wherein, described reinforcement composition contains ATCC55675.
37. a method that increases the setting number of sites of solid plant, this method is with effective active component plant growth regulator on (a) agricultural; (b) brood cell of a kind of suitable bacillus or soil bacteria, culture or suspension are strengthened composition, spray application on the blade face of described plant, its prerequisite is that when described plant growth regulator was a kind of plant growth inhibitor, said composition did not contain auxin.
38. as the method for claim 37, wherein, effective active component and described reinforcement composition use with composition forms on the described agricultural.
39. as the method for claim 37, wherein, described solid plant is a cotton.
40. as the method for claim 37, wherein, described plant growth regulator is a plant growth inhibitor.
41. as the method for claim 37, wherein, described plant growth regulator is a plant growth promoter.
42. as the method for claim 37, wherein, described reinforcement composition contains the bacterial strain from the bacterium of bacillus.
43. as the method for claim 37, wherein, described reinforcement composition contains the soil bacteria bacterial strain.
44. as the method for claim 37, wherein, described reinforcement composition contains Bacillus cercus.
45. as the method for claim 37, wherein, described reinforcement composition contains the Bacillus cercus of the feature with ATCC55675.
46. as the method for claim 37, wherein, described reinforcement composition contains bacillus subtilis.
47. as the method for claim 37, wherein, described reinforcement composition contains the bacillus subtilis of the feature with ATCC55675.
48. as the method for claim 37, wherein, described reinforcement composition contains bacillus megaterium.
49. as the method for claim 37, wherein, described reinforcement composition contains ATCC55675.
50. a method that strengthens the effect of weed killer herbicide, this method are by giving effective active component plant growth inhibitor or weed killer herbicide on a kind of agricultural of plant-spraying (a); (b) a kind of suitable bacillus or brood cell, culture or the suspension of soil bacteria are strengthened composition.
51. as the method for claim 50, wherein, effective active component and described reinforcement composition are to use with composition forms on the described agricultural.
52. as the method for claim 50, wherein, effective active component is triazine, glyphosate or sulfosage on the described agricultural.
53. as the method for claim 50, wherein, described reinforcement composition contains the bacterial isolates from bacillus.
54. as the method for claim 50, wherein, described reinforcement composition contains the soil bacteria bacterial strain.
55. as the method for claim 50, wherein, described reinforcement composition contains Bacillus cercus.
56. as the method for claim 50, wherein, described reinforcement composition contains the Bacillus cercus of the feature with ATCC55675.
57. as the method for claim 50, wherein, described reinforcement composition contains bacillus subtilis.
58. as the method for claim 50, wherein, described reinforcement composition contains the bacillus subtilis of the feature with ATCC55675.
59. as the method for claim 50, wherein, described reinforcement composition contains bacillus megaterium.
60. as the method for claim 50, wherein, described reinforcement composition contains ATCC55675.
61. a composition that improves the activity of plant growth regulator, said composition contain a kind of suitable bacillus and brood cell, culture or suspension, also comprise a kind of plant growth inhibitor, wherein, said composition does not contain ATCC55675.
62. as the composition of claim 61, wherein, described plant growth regulator comprises helping strengthens element.
63. as the composition of claim 61, wherein, described plant growth regulator comprises Chlormequat.
64. as the composition of claim 61, wherein, described bacillus is a Bacillus cercus.
65. as the composition of claim 61, wherein, described bacillus has the feature of ATCC55675.
66. as the composition of claim 61, wherein, described bacillus is a bacillus subtilis.
67. as the composition of claim 61, wherein, described plant growth regulator is a plant growth inhibitor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 96180578 CN1244771A (en) | 1996-11-29 | 1996-11-29 | Agricultural compositions containing bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 96180578 CN1244771A (en) | 1996-11-29 | 1996-11-29 | Agricultural compositions containing bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1244771A true CN1244771A (en) | 2000-02-16 |
Family
ID=5128009
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 96180578 Pending CN1244771A (en) | 1996-11-29 | 1996-11-29 | Agricultural compositions containing bacteria |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1244771A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102065697A (en) * | 2008-04-07 | 2011-05-18 | 拜耳作物科学公司 | Combinations of biological control agents and insecticides or fungicides |
| CN103889235A (en) * | 2010-12-10 | 2014-06-25 | 奥本大学 | Inoculants including bacillus bacteria for inducing production of volatile organic compounds in plants |
| CN107058189A (en) * | 2017-06-05 | 2017-08-18 | 青岛农业大学 | Bacillus megaterium and its processing heavy metal glyphosate combined pollution in apply |
| CN108282996A (en) * | 2014-12-29 | 2018-07-13 | Fmc有限公司 | For being applied in combination with soil insecticide to promote the microbial composite of plant growth |
| CN109234187A (en) * | 2018-08-15 | 2019-01-18 | 李晓明 | One plant of atrazine degradation bacteria and its application |
-
1996
- 1996-11-29 CN CN 96180578 patent/CN1244771A/en active Pending
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102065697B (en) * | 2008-04-07 | 2017-07-14 | 拜耳知识产权有限责任公司 | The composition of biocontrol agent and insecticide or fungicide |
| CN105580844A (en) * | 2008-04-07 | 2016-05-18 | 拜耳知识产权有限责任公司 | Combinations Of A Biological Control Agent And Insecticides |
| CN102065697A (en) * | 2008-04-07 | 2011-05-18 | 拜耳作物科学公司 | Combinations of biological control agents and insecticides or fungicides |
| CN105580844B (en) * | 2008-04-07 | 2020-01-03 | 拜耳知识产权有限责任公司 | Composition of biological control agent and insecticide or fungicide |
| CN103889235A (en) * | 2010-12-10 | 2014-06-25 | 奥本大学 | Inoculants including bacillus bacteria for inducing production of volatile organic compounds in plants |
| US9282745B2 (en) | 2010-12-10 | 2016-03-15 | Auburn University | Inoculants including Bacillus simplex for inducing production of volatile organic compounds in plants |
| CN103889235B (en) * | 2010-12-10 | 2016-11-23 | 奥本大学 | The Inoculant comprising bacillus (BACILLUS) bacterium producing for induction VOC in plant |
| US9615583B2 (en) | 2010-12-10 | 2017-04-11 | Auburn University | Inoculants including Bacillus bacteria for inducing production of volatile organic compounds in plants |
| CN108282996A (en) * | 2014-12-29 | 2018-07-13 | Fmc有限公司 | For being applied in combination with soil insecticide to promote the microbial composite of plant growth |
| CN107058189A (en) * | 2017-06-05 | 2017-08-18 | 青岛农业大学 | Bacillus megaterium and its processing heavy metal glyphosate combined pollution in apply |
| CN107058189B (en) * | 2017-06-05 | 2019-05-28 | 青岛农业大学 | Bacillus megaterium and its processing heavy metal-glyphosate combined pollution in apply |
| CN109234187A (en) * | 2018-08-15 | 2019-01-18 | 李晓明 | One plant of atrazine degradation bacteria and its application |
| CN109234187B (en) * | 2018-08-15 | 2021-06-15 | 李晓明 | Atrazine degrading bacterium and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1091765C (en) | Novel 5-amino-3-cyano-4-ethylsulfinyl-1-phenyl-pyrazole compounds and their use as pesticides | |
| CN1568296A (en) | Micro-organisms for the treatment of soil and process for obtaining them | |
| CN1113604C (en) | Use of streptomyces WYEC 108 to control plant pathogens | |
| CN1162083C (en) | Pesticide | |
| CN1479577A (en) | Synergistic bioinoculant composistion comprising bacterial strains of accession NosNEEL B-30486, NRRL B-30487and NRRL B-30488 and method of producing said composition thereof | |
| CN1276007A (en) | Competitive exclusion culture for swine | |
| CN1531395A (en) | Method of improving yield and vigor of plants | |
| CN1145695C (en) | Antifungal protein | |
| CN1251131A (en) | Enhancement of growth in plant | |
| CN1074900C (en) | Biological control of insects | |
| CN101054568A (en) | Microorganism bacterium composition, special biological organic fertilizer for tobacco containing the same, preparation and use method for the microorganism bacterium composition | |
| CN1246890A (en) | Antifungal biocontrol agents, process for preparing and treating the same | |
| CN1463604A (en) | Pest control agent comprising multiple micro-organism | |
| CN88101628A (en) | The synthetic substrate that is used for filamentous fungus | |
| CN1102958C (en) | Microorganisms for biological control of plant diseases | |
| CN87103967A (en) | 1,2,3,6-tetrahydrochysene-5-nitro-pyrimidine derivatives | |
| CN1291078A (en) | Biocontrol agent for control of root diseases | |
| CN1350541A (en) | Plant activator, process for producing the same, actwation method, activity promoter and method for applying the promoter | |
| CN1016840B (en) | Physiologically active agent for agricultural use | |
| CN1904035A (en) | Combined nitrogen fixing phosphorus disintegrating potassium disintegrating micro organism composite fertilizer | |
| CN1037067A (en) | The Synergistic biocidal and the miticide composition that contain two or three active component | |
| CN86107988A (en) | Biological nitrogen fixation in the non-leguminous plant | |
| CN1244771A (en) | Agricultural compositions containing bacteria | |
| CN1102858A (en) | New variety of drechslera monoceras, weed control compositions containing the same as an effective ingredient and weed control methods using the same | |
| CN1110708A (en) | Bacterial preparation for agricultural use no priority right |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| REG | Reference to a national code |
Ref country code: HK Ref legal event code: WD Ref document number: 1025881 Country of ref document: HK |