CN1110708A - Bacterial preparation for agricultural use no priority right - Google Patents

Abstract

The agricultural bacterial preparation for soil improvement contains the Bacillus' bacteria for generating lipopeptide to reduce surface tension of water and under anaerobic condition, able to reproduce in soil containing plant fibre, the Bacillus' or Fusiformis' bacteria for generating cellulase and able to reproduce in soil containing plant fibre, and the preferred Bacillus' or Fusiformis' bacteria for fixing nitrogen and under anaerobic condition, able to reproduce in soil containing plant fibre.

Description

Bacterial preparation for agricultural use no priority right

The invention relates to the bacteria preparation that on the agriculture field of arviculture, cattle breeding, uses.In more detail, the invention relates to soil improvement bacteria preparation, the feed efficiency promotor of the reodorant of livestock waste and domestic animal into plant growing.

In plant growing, existing many kinds of soil improvement agents are used to boost productivity, and some of them are microbial preparations, and many in them are aerobic preparations.Though the fertilizer of fully fermenting (high quality fertilizer) can utilize these conditioning agent production, but most of peasants are not positive to the mixed fertilizer that promptly uses these aerobic microorganism preparations to produce, its reason has following: when at first in mixed fertilizer processing, using them, and essential ventilation and mixing in particular facility; The second, need more facility expense and more labour, meanwhile, in fertilizer processing, can produce stench.In addition, be used for common farmland as the mixed fertilizer that will finish fermentation, then annual every 10a ground needs to drop into several tons of this fertilizer and uses continuously and just can reach required result in several years, and promptly competent humic forms suitable granule degree etc.Therefore, this soil improvement method only just can be accomplished by small portion typical case peasant.

Some are used for the disappear anaerobion of smelly purpose of plant growing, animal manure is known.But, these microorganisms, generally speaking the overwhelming majority is made up of the vegetative propagation cell, and major part is not told from soil.Therefore when being applied in soil, domestic animal, the persistence of effect, use neutralization stable not ideal enough in preserving.It is wideless to exist purposes, and access times, usage quantity are more, shortcomings such as effect, deficiency in economic performance.

Once the someone used the single bacterial strain of bacillus and Pseudomonas, make cellulosic become thoroughly decomposed promotion, soil improvement, fecaluria disappear smelly and feed efficiency promotes be the bacteria preparation of purpose, did trial sale or tested.But, all fail on market, to take root and disappear.This because, soil, animal manure, domestic animal digester tube content etc., conditions such as its composition, potential of hydrogen, moisture differ separately, therefore, it is impossible using single bacterial strain to satisfy various uses, adapting to various conditions.

In recent years, the high-yield variety that is used on many farm crop is gone on the market, and the peasant wishes by reaching high yield with these kinds.But the peasant seldom uses mixed fertilizer (or filamentary material), but uses chemical fertilizer too much, the result be with the microbiota in the soil change into up-set condition and since microbial reproduction be easy to cause special disease.Just be to use a large amount of fumigant and sterilant controlling these microorganisms then, and the composition in the chemical fertilizer builds up in soil, the infringement that causes of farming has taken place continuously.At present, there is not known means to finish this infringement of prevention.

At present, though prevent animal house fecaluria malodorant,, these preparations, the root that cut-out stench that can not essence takes place.Therefore, disappear smelly effect, economic benefit is all undesirable.At present, also do not see, from the fresh fecaluria that animal house is collected, directly use the farmland at once after, do not produce any harm of growth of the roots of farm crop and farm crop, and preparation with essence effect.In addition, though have some antiseptic-germicides to can be used to promote feed efficiency as fodder additives,, also do not see at present, can essence when promoting feed efficiency prevent the malodorous preparation of livestock waste.

The present invention relates to the soil improvement bacteria preparation, it comprises can produce the lipopeptid with water surface tension reduction effect, and have can be under anaerobic, the bacillus of in the soil that vegetable fibre exists, breeding (producing bacterium) hereinafter to be referred as lipopeptid, and production of cellulose enzyme and have can be under anaerobic, bacillus that breeds in the soil that sponge exists or fusobacterium bacterium (producing bacterium hereinafter to be referred as the plain class of cellulase).

The invention still further relates to the soil improvement bacteria preparation, it comprises lipopeptid production bacterium, and cellulase production bacterium and energy fixed nitrogen also can be under anaerobic, the bacillus or the fusobacterium bacterium that breed in the soil that plant cellulose material exists.

The invention still further relates to by aforementioned lipopeptid production bacterium and the plain class of aforementioned fibers and produce the livestock waste deodorant that bacterium is formed.

The invention still further relates to by aforementioned lipopeptid production bacterium and the plain class of giving birth to of aforementioned fibers and produce the cattle food efficient promotor that bacterium is formed.

Lipopeptid (lipopeptide) also claims peptiolipid (peptidelipid), generally comprises the amino acid of several ring structures and encircles the material of terminal bonded fatty acid structure at these, and it is a kind of have hydrophilic group and hydrophobic group tensio-active agent.In the present invention, selectivity uses those lipopeptids to produce bacterium, and it can secrete lipopeptid, and lipopeptid can be reduced to lowest surface tension (MN/m) in its aqueous solution and be less than 40, and preferred 35(is in 25 ℃, the value that is recorded by Fisber Autoten-siomat).

The typical example that these lipopeptids are produced bacterium has bacteriums such as subtilis, Bacillus licheniformis, Bacillus circulans, and they all are sporeformers.

The Mierocrystalline cellulose enzyme of being carried in the present invention means, to the edaphophyte cellulose materials, promptly, remaining cauline leaf after the farm crop results, stalk class, fallen leaves, husk, sawdust, wood shavings, wooden shavings, bark etc., cellulase (cellulase), hemicellulase (hemicellulase), polygalacturonase enzymes such as (pectinase) with Degradation.

The typical example that these fiber enzymes are produced bacterium has: subtilis, Bacillus licheniformis, Bacillus circulans, bacillus polymyxa, Bacillus coagulans, soak bacteriums such as numb genus bacillus and Clostridium cellulolyticum, Cl.aerotolerans, clostridium acetobutylicum.It all is the spore bacterium.

All bacterium of using among the present invention are under anaerobic, reproducible bacterium in the soil in the presence of the vegetative fiber material.Because these bacterium are isolated and have the sporulation ability from soil, so these bacterium are highly stable in preservation, and can vigorous breeding in soil, animal manure, feed.

In the present invention, if, when same bacterial strain can be produced lipopeptid and cellulase, or same bacterium is can the production of cellulose enzyme time, when having nitrogen fixing capacity, as far as possible preferably use different strains,, can breed under the composition, potential of hydrogen, temperature, moisture content as them so that they are under the different condition of the digestion organs content of soil, animal manure, domestic animal.Say that more specifically bacteria preparation of the present invention should produce bacterium being selected from lipopeptid, several or a few strain bacteriums of cellulase generation enzyme and vinelandii combine and are used for each purpose.

When wanting to degrade effectively (becoming thoroughly decomposed) vegetative fiber matter, need the carbon element rate (C/N ratio) of certain limit.But, Zuo Yong vinelandii in the present invention, can fixed air in soil in nitrogen, therefore, do not replenish nitrogenous fertilizer in addition and also can promote cellulosic degraded.

The typical example of vinelandii in the present invention has: Bacillus azotofixans, invade bacteriums such as numb genus bacillus, bacillus polymyxa and clostridium acetobutylicum, Pasteur's gemma clostridium.They all are that the spore bacterium is arranged, and can not produce collaborative biological action with roots of plants.

In its aqueous solution, can reduce the surface tension of water at the lipopeptid of the present invention's use powerfully.That is, reduce the viscosity of water, increase mobile, thereby the micro-pore of water by biological surface can be invaded cell interior.The excretory lipopeptid is easy to become solution in water in soil.The water that contains lipopeptid by the micro-pore on fibrocyte surface, is invaded the intrusion pressure of following them, and they further enlarge the diameter of micro-pore.Therefore, normocrinic by other microorganism, can the degrading plant cell walls and the Mierocrystalline cellulose enzyme of intercellular substance, can pass through extended hole, invade cell interior effectively, promote the degraded of cell walls and entocyte.

In addition, excretory lipopeptid in the soil, in the dissolving water after, micro-pore that can be by clay particle surface is with water intrusion.Therefore, they can littleization clay particle, owing to granule be that complicated littleization clay particle is formed, and little chemical fibre dimension cellulosic material is by cellulose degraded, therefore, and the formation that lipopeptid has promoted granule.Lipopeptid, (lipoanino-acid) compares with the lipoamino acid with similar effect, and the stability in soil is higher.

Because an outside air permeability is good, become the Steady breed place of the aerophil of perch in soil.On the other hand, the aerobism bacterium at the process consumption oxygen of the outside breeding of granule, is promoted an inner anoxic condition, and an inside becomes the Steady breed place of anerobe.

Under micro-situation, aerobic space and anaerobism space existence near each other in the soil, lipopeptid is produced bacterium for this and the Mierocrystalline cellulose enzyme is produced bacterium, and stable breeding place is provided, and being becomes the key factor of keeping each bacteria paragenesis breeding for a long time.

Lipopeptid is vigorous secretion under the anaerobic condition in soil, and is especially, more vigorous in fiber degradation initial stage (arriving fiber softening with the last stage).After decomposing further progress, promptly reduce the secretion of lipopeptid, that is, the lipopeptid secretory volume in the complete ripeness compost is also few.Therefore, finish the field of the mixed fertilizer of fermentation and drop into, the granule of not necessarily giving full play to lipopeptid forms facilitation effect.

Because unfermentable filamentary material can produce phenol and volatile nitrogen material to the crop root part infringement in degradation process, therefore say that generally the fertilizer of fully fermenting can not use.But the peasant is selected from the preparation of the present invention of not secreting the objectionable impurities bacterium as using the present invention, then not only the filamentary material commitment can vigorous secretion lipopeptid and also the objectionable impurities of the fiber that can prevent from not ferment produce.In other words, when suitably using preparation of the present invention, the formation that can promote granule is even and produce in filamentary material degraded commitment also can prevent from the not ferment objectionable impurities of filamentary material.

For avoiding the above-mentioned infringement problem in the continuous farming, improve productivity, the secular stable high yield of maintenance of soil, necessary will control spreading of specific pathogeny bacterium.For this reason, high-caliberly maintain that the breeding of multiple microorganism is necessary in the soil.For microorganism provides the good environment that adapts to vigorous breeding, just mean a promotion that forms and under the condition of microbial reproduction long term maintenance they.

Because lipopeptid slowly decomposes in soil, therefore, a formation is also destroyed gradually.The destruction of granule is also quickened in the field farming.Want to keep the above granule of certain level and form, need to continue a certain amount of new lipopeptid of supply., continue to drop into, the bacterium and a certain amount of undecomposed cellulosic that can play long term in soil, have reliable effectiveness are necessary for this reason.

In the present invention, bring into play the smelly effect of disappearing of fecaluria by following method.Find the bacterium to produce lipopeptid in the digester tube of domestic animal.Produce bacterium though in internal organ, found the Mierocrystalline cellulose enzyme,, the Mierocrystalline cellulose enzyme that does not have lipopeptid to exist can not be given full play to forceful action.That is, the cell walls of the vegetable cell in the feed of can not degrading, intercellular substance (forming) by polyose, most of feeds are along with ight soil is discharged from by original state.If, spreading fertilizer over the fields on the ight soil and of the present inventionly disappear smellyly when using bacteria preparation, the output lipopeptid works to cell walls and intercellular substance remaining in the ight soil, enlarges its surperficial micro-pore.For this reason, make the Mierocrystalline cellulose enzyme of coexistence produce bacterium excretory Mierocrystalline cellulose enzyme raising effect, effectively, thinlyyer cell walls and intercellular substance are degraded to littler composition.As a result, can reduce the positive fusobacterium bacterium (4 * 10 of repugnant substance generation main cause-indoles (indole) that (comprises in the excrement) perch in the domestic animal large intestine significantly ⁵→ 3 * 10 ³/ gram), on the other hand, increase considerably the negative fusobacterium bacterium (2 * 10 of the indoles that repugnant substance does not take place ³→ 3 * 10 ⁵/ gram) perch.Though similar effect is produced bacterium by lipopeptid ight soil is spread fertilizer over the fields also and can reach, (the positive fusobacterium bacterium (4 * 10 of indoles ⁵→ 2 * 10 ⁴/ gram), but that its effect be it seems is undesirable, unless it produces bacterium when shared, the ability reinforced effects with the fiber enzyme.

Aforementioned bacterium of the present invention has the characteristic of the volatility helium composition that do not produce the stench reason, hydrogen sulfide, indoles.Say that more specifically because bacterium of the present invention can consume repugnant substances such as the ammonia that exists, hydrogen sulfide, indoles, butanic acid in ight soil, therefore, these bacteriums can be eliminated the distinctive stench of animal house.

Preparation of the present invention can promote feed efficiency by following method.Phyteral is as the main raw material of cattle food, and compositions such as protein wherein, starch are closed in cell walls, and these compositions are degraded by the various digestive ferments that can invade cell walls.But under the normal circumstances, lipopeptid produces the interior breeding of intestines that bacterium is stayed out and raises, and therefore, the efficient that digestive ferment penetrates cell walls and intercellular substance is not high, and the degraded of protein, starch etc. is abundant inadequately.But,, lipopeptid can make several digestive ferments be easy to invade in the cell by cell walls because of producing bacterium owing to expanded the cell walls micro-pore from bacterium excretory lipopeptid.(lipopeptid produces bacterium can not constant breeding in pluck: they can be used as the breeding of transition bacterium when continuous oral).Therefore, protein and starch in cell at first the degraded, their molecular weight be degraded to enough little after, the material of degraded is very easy to overflow cell by micro-holes.In the cell outside, protein and starch degradation are further promoted by digestive ferment in the feed then.The method of Here it is enhancing digestion or feed efficiency.

By aforesaid method, oral bacteria preparation also can suppress the generation of repugnant substance in intestines.The result greatly reduces the stench of ight soil and assists to have alleviated the physiological load of domestic animal, therefore further improves the utilization ratio of feed.Though perch Mierocrystalline cellulose enzyme is produced bacterium in the domestic animal intestines, lipopeptid produces bacterium and the Mierocrystalline cellulose enzyme is produced bacterium and time spent, and this effect is more remarkable.

The above is to the description of soil improvement of the present invention with preparation, disappear smelly usefulness preparation and feed efficiency promotion usefulness preparation role.Though by forming with the same bacterium, lipopeptid production bacterium and Mierocrystalline cellulose enzyme production bacterium are united use and can enough satisfy each purpose basically for they.

When using soil improvement agent of the present invention, also can be incorporated in the soil, as required, also an amount of nitrogenous source that is used to ferment can be executed at an upper portion thereof as the cellulosic of bacteriotrophy thing.In order to keep anaerobic condition, must fully cover them with soil and contact with air avoiding.Usually, lipopeptid is produced bacterium, after week, begins breeding through 1-2 at Mierocrystalline cellulose with on as nutraceutical nitrogenous source.The bacterium of the Mierocrystalline cellulose enzyme of symbiotic relationship production meanwhile also begins breeding, has strengthened the formation of cellulosic degraded and granule thus.

The other method of improving edaphic condition with preparation of the present invention is after said preparation is incorporated in the fiber material fiber material short period of time to be piled up, and then they is used as fertilizer, and this can use on the fiber material such as bark of difficult fermentation.Also can after preparation of the present invention applies on the littering of animal house, littering of recovery be applied on the soil.In addition, the fecaluria of the animal of oral preparation of the present invention also can be applicable on the soil.

Say that more specifically this preparation is all right, spread fertilizer over the fields on the animal house fecaluria, or, sneak into cattle food and use as feed efficiency promotor as deodorant.

In the animal house of chicken, pig, ox etc., often use straw, sawdust etc. as auxilliary grass on the ground.In animal house as the preparation of the present invention of the deodorant of fecaluria, when on these litter, spreading fertilizer over the fields preparation of the present invention in right amount, behind certain hour (2-3 week usually), by consume as nutrient source litter with excrement in the remaining lipopeptid that does not digest in cellulosic and nitrogen composition and the said preparation produce bacterium, meanwhile, Mierocrystalline cellulose enzyme in the said preparation is produced bacterium and is also begun breeding with symbiotic relationship, and beginning actively breeding and suppress in the ight soil, bacterium takes place in the stench in the digester tube.

Because, do not exist in the animal waste as exist in the granule in the soil the micro-pore that is used to breed bacterium, therefore, in animal waste, often shorten the bacterium life-span in the said preparation.For more economical, take said preparation and porous matter silicic acid matter (zeolite, diatomite, green tuff etc.) to spread fertilizer over the fields on the excrement together, like this, bacterium energy Steady breed makes their prolongs lifes, thereby saves usage quantity and access times.

Granule the small degradation product of formation degree, cellulosic (corruption plant etc.) how much be to judge the principal element whether plant growing soil (ploughing native) is enhanced.In the present invention,, adopted this area to be considered to adapt to and the loading capacity (CEC) and the conductivity (EC) of widespread use most as the index of determine effect.As abundant formation granule and small degradation of fibers material, then the CEC value of Geng Diing has increased, and this is because the surface-area of these particles has enlarged and can absorb whole and more fertilizer composition (positively charged ion and negatively charged ion).On the contrary, reduce the fertilizer composition of not sorption, cause the reduction of the EC numerical value of ploughing soil.But these numerical value can produce difference according to the difference of ploughing soil, therefore, should note the relative value in the same test.

Feed efficiency is the heavy ratio of feed intake (air-dry thing conversion) and Livestock Production thing, is the index of the food utilization efficiency of livestock products things such as expression meat, egg, milk when producing.That is, this numerical value is big more shows that the utilization ratio of feed is high more.

The bacterium of Shi Yonging is in the present invention cultivated under substratum that bacterium separately adapts to and condition of compatibility, makes them form spore as much as possible.If, when having the vegetative cell beyond the spore, usually and they dry together, then,, adjust separately the bacterium of bacterium according to purpose and count ratio, and make anhydrous formulation with the forming agent of adaptation.

The spore of collecting from substratum of the present invention can carry out according to a conventional method.The substratum that the present invention uses can contain any nutrition source of being useful on bacterium of the present invention such as various carbon source, nitrogenous source, inorganic metal salt source.

Especially when cultivating the bacillus bacterium, preferably use the Sifco sporulation substratum (Difco Sporulation Medium) or the similar substratum of Difco society.When cultivating the cultivation of fusobacterium bacterium, consider this genus bacterium relatively poor characteristic of sporulation ability on the eutrophy substratum, preferably use, for example, (Long S.et al such as Long; Appl.Environ.Mi-crobio., 45 1389-1393,1983) minimal medium such as CAMM:Clostridium acetobutylicum Sporulaion Minimal Medium or similar substratum.

For the breeding of bacterium of the present invention, can adopt aeration-agitation commonly used to cultivate, also can take solid culture method.After cultivate finishing, the separating of thalline and substratum can take the separation method used always promptly, centrifugal separation, filtration method, membrane separation process etc. still, when needing to keep centrifugal condition, are preferably taked centrifugal separation.

When needing spore to separate with vegetative cell, as required, method such as the thermal treatment that can take to use always, N,O-Diacetylmuramidase (lysozyme) processing, alcohol processing is destroyed vegetable cell.

Oven dry is to make the preceding last process of said preparation, can take methods such as warm air drying, spraying drying, lyophilize.

Bacteria preparation of the present invention can be made by mixing with appropriate carrier according to the above-mentioned dry bacterial of obtaining.Kind of carrier does not have particular restriction, the powder of silicic acid matter, and for example, zeolite, diatomite, green travertine etc. are all passable.

Though the suitable total count in the bacteria preparation of the present invention is different variant with bacterial classification, general range is 10 ⁴-10 ¹⁰/ gram, 10 ⁵-10 ⁸/ restraining to more satisfactory, spore count is 10 ⁴-10 ⁸The scope of/gram, 10 ⁴-10 ⁶/ restrain to more satisfactory.

The usage quantity of bacteria preparation of the present invention is not crucial, and can determine flexibly according to object, condition etc.The usage quantity of soil improvement agent changes according to dropping into cellulosic amount, and its scope is every 10a 50-400kg, and 100-200kg is more satisfactory.The usage quantity of deodorant is that when executing 1 time in 7-8 days, it is more satisfactory that animal house is executed 20-160g, 40-80g for every square metre.As feed efficiency promotor, can be by adding 0.03-0.3% in the feed, 0.05-0.2% is more satisfactory.

Following examples are used for further specifying the present invention.

The manufacturing of embodiment 1 dry bacteria preparation

Produce subtilis ATCC 21332 and Bacillus licheniformis ATCC 39307, subtilis ATCC 6051, the Bacillus licheniformis ATCC 14580 of production of cellulose enzyme and the bacterium of bacillus such as Bacillus circulans ATCC 9500 and sticking genus bacillus ATCC 842 of lipopeptid, use Difco sporulation substratum (the Difco Sporulation Medium that adapts to sporulation, Difco society), cultivate respectively in following condition.Incubation time according to the cultivation progress of bacterium separately, is suitably adjusted in 12-48 hour scope.

Culture apparatus: 1 liter of 2 liters of miniature fermenters (Mitsuwa physics and chemistry system) substratum

Culture condition: 1 liter/minute of air flow

Stir 400RPM

37 ℃ of temperature

Incubation time 12-48 hour

After cultivating end, carry out centrifugation (10,000G, 20 minutes), collect bacterial cell, carry out lyophilize, make drying agent according to well-established law.Drying agent, under aseptic condition, to pulverize, a certain amount of being suspended in 0.85% normal saline solution wherein asked total count after cultivating with plain agar substratum (Japanese pharmacy society system).In addition, after the thermal treatment in 85 ℃, 15 minutes, cultivate equally, measure spore count.

The cultivation of fusobacterium bacterium needs anaerobic condition.At the Cl.cellulolyticum of production of cellulose enzyme A TCC 35319, in the cultivation of Cl.aerotorerans ATCC 43524, use (CAMM:Clostridium acetobutylicum Sporula-tion Minimal Mediium) such as Long, cultivate respectively by following condition.Incubation time and last same.

Culture apparatus: 1 liter of 2 liters of miniature fermenters (Mitsuwa physics and chemistry system) substratum

Culture condition: 1 liter/minute of air flow

Stir 400RPM

37 ℃ of temperature

Incubation time 12-48 hour

After cultivating end, under the anaerobic condition that nitrogen exists, carry out centrifugation and collect bacterial cell.Under anaerobic carry out drying, afterwards drying agent nitrogen is preserved down.Said preparation is pulverized under aseptic condition, then, except using CAM bouillon(Japan pharmacy society system), add agar plate, and beyond under anaerobic cultivating, measure total count and spore count according to above-mentioned same quadrat method.

Have the cultivation of the bacillus bacterium and the fusobacterium bacterium of nitrogen fixing capacity, carry out in the following order.Belong to bacillus Bacillus azotofixans ACTT 35681, soak numb genus bacillus ATCC 8244 and cultivate, collect by the method for above-mentioned genus and dry.

The bacterium that must need the fusobacterium of anaerobic condition during cultivation, is cultivated, collects and dry according to the culture condition of above-mentioned anerobe Clostridium aceto-butylicum ATCC 824, Cl pasteurianum ATCC 6013.Under aseptic condition, pulverize above-mentioned preparation, measure total count and spore count by the method for above-mentioned bacillus bacterium, fusobacterium bacterium.

The preparation of embodiment 2 bacteria preparations

The dry bacteria preparation that obtains at embodiment 1 suitably mixes and mixes with zeolite the bacteria preparation 1-6 that obtains adjusting to the bacterium number of hoping.The bacterium of 1-6 bacteria preparation is formed oblatio at table 1.

The present invention further describes by following application.

Application examples 1: the embodiment of soil improvement agent

Farm crop: wheat breed: No. 61, agricultural

Culture time; Late October sowing, results mid-June in next year (group Ma Xian)

The fiber material

Straw

Test site: 530

Listing production area: 530(kg/10a)

Fermentation urea: test site: 5(kg/10a), listing production area: 4(kg/10a) bacteria preparation: test site: the bacteria preparation 1 of application examples 2: listing production area: use SU-PER KARUSON NR-C(soil improvement anerobe preparation, ClBA B10 fermentation KK society system, total count: 3 * 10 ⁸/ gram).Consumption: test site: 10; Listing production area: 20(kg/10a).Bacteria preparation is set does not in addition have application zone.3 districts of the Fertilization Level of n p k fertilizer all consistent (, not having the same chemical fertilizer level of filling person) in following plant growing test.

Use situation; After the straw spreading of producing per year, spread fertilizer over the fields preparation and urea equably, be embedded in the dark soil of 10-15cm, cover with soil.Use sowing after 1 day.No application zone straw does not have input yet.

Result's general introduction;

Output: test site 405, listing production area 382 does not have the district of use 345(kg/10a).

Thousand seed weight: test site 37.5, listing production area 36.8 does not have the district of use 36.1(g).

Index; (with down to test example 7, all the measured value of the soil of gathering from the dark part of face of land 15cm) measured value at the beginning of 6 months.

CEC: test site 13.6, listing production area 11.2, no application zone 8.9(me/100g)

EC: test site 0.2, listing production area 0.3, no application zone 0.7(ms/cm[1:5]).

Illustrate: the wheat of test site and listing production area, root growth is good, takes root tightly, and is later because of phase in spring jointing, avoided the harm of late frost.Two growths of district after equal May are smooth.The wheat of listing production area, although use 1 times preparation than the test site more,, the rate ratio test site is poor slightly.No application zone, the growth of root is poor, and phase in spring jointing is very fast, and the late frost that runs into late April is killed, and growth is afterwards postponed to some extent, has reduced output.

Analyze:, learn the degree of soil improvement by near the results CEC and the EC numerical value in period.Though the bacterium of preparation passes the winter,, in the test site, from the spring to the early summer, the activity of bacterium is active, so soil has obtained enough improvement.Show on the output and thousand seed weight of wheat.

The embodiment of application examples 2 soil improvements

Farm crop: rice varieties: KINUHIKARI

Culture time: (Nagano county) gathered in the crops in rice transplanting by the end of October at the beginning of 6 months

Fiber material: straw:

Test site: 300

Listing production area: 300(kg/10a)

Rice hulls:

Test site: 200

Listing production area: 200(kg/10a)

Fermentation urea: test site: 5

Listing production area: 4(kg/10a)

Bacteria preparation: test site: the bacteria preparation 2 of application examples 2, listing production area: use same preparation in the application examples 1.Amount of application, test site, listing production area are 10(kg/10a), preparation is set does not in addition have application zone.

Preparation uses: cellulosic is dropped into equably all rice transplanting harrowing a field period before 3 days in test site, listing production area, spreads fertilizer over the fields preparation and urea then equably.Be embedded in 10-15cm part in the soil with cellulosic then.Do not have to execute and use the district, cellulosic does not have input yet.

Result's general introduction;

Every strain effective tillering number: test site 29.3, listing production area 26.7, nothing are used district 22.3.

Brown rice output: test site 684, listing production area 648 does not have the district of use 633(kg/10a).

Brown rice thousand seed weight: the test site 20.8. production area 20.6 of going on the market, do not have the district of use 20.6(g).

Index: the numerical value at the beginning of 8 months

CEC: test site 31.6, listing production area 26.4, no application zone 23.6(me/100g)

EC: test site 0.05, listing production area 0.2, no application zone 0.7(ms/cm[1:5]).

Illustrate: think that always like this, transplanting date is during near the planting type of early summer, if dropped into cellulosic before early summer, along with anaerobically fermenting is quickened in the rapid rising of water temperature, because the generation of hydrogen sulfide, biogas, foamed phenomenon occurs, crop root is produced infringement, influence the healthy growth of crop.But,,, do not produce foamed phenomenon though the cellulosic material used in rice transplanting in preceding 3 days in the test site.This is because the bacterium in the preparation secretes lipopeptid and Mierocrystalline cellulose enzyme, and promptly decomposes cellulosic powerfully, and the cause that does not produce the root objectionable impurities in the process that cellulosic decomposes.On the other hand, in the listing production area, the foamed phenomenon by the anaerobically fermenting cause appears in early summer.This reason is that degradation of fibers mass-energy power is relatively poor, and fails promptly to decompose cellulosic, and to the generation of root objectionable impurities.Thus, postponed growth, compared with the test site, minimizing by a relatively large margin tiller number and output.

Analyze: the early August after 2 months on-tests, from the dark soil of each about 1.5cm in district, take soil, the 100g sample in each district is put beaker into 400ml water, fully after the stirring, puts into the graduated cylinder of 5 * 30cm.Though the macroparticle of soil promptly precipitates, continue to suspend at the top fine particle.Measure the transparent required time of arrival of top 6cm part.Arriving the transparent time is, no application zone 4.5 hours, listing production area 18 hours, in the test site, through 30 hours also not transparent.This means, by miniaturization, form small at test site clay particle.This conclusion also can be proved by CEC, EC numerical value.Because, paddy field soil clay content height, therefore, the CEC value in 3 districts is all high, and still, the numerical value of test site is outstanding, and a progress that forms is described.

Application examples 3 soil improvement embodiment (example that uses after the composting)

Crop: melon variety: ANDES

Bacteria preparation: the test site, the bacteria preparation 3 of application examples 2, the listing production area is used and application examples 1 same preparation.Preparation is set does not in addition have application zone.

Test period; Mixed fertilizer heap matter begins: the test site: early March, and the listing production area, late Febuary, no application zone, early September begins.Cultivation, 3 districts are all from early March.(outstanding Yu Xian)

Preparation uses: collect in the kitchen, center at chain restaurant, is mainly vegetables, fruit, and part contains the mixed fertilizer at the not edible position of fish, meat, and each collects 4 tons (C/N is than 30-35, moisture 75-80%).Each 2kg of bacteria preparation, 4kg with porous matter (the diameter 3-30 μ m in hole) each 40kg of zeolite powder and 4 tons of mixed fertilizer thorough mixing, are piled into coniform, surperficial with plastic covered blocking air on concrete floor.Original state was stacked each 4 days and 8 days.No application zone is piled up mixed fertilizer by original state, per 2 weeks have piled up 6 months all stirring ventilation.Through the mixed fertilizer that these processing obtain,, directly use on the arable land of not having the plastic greenhouse of heating by the ratio of every 10a/4 ton.Execute the back and cover 10-15cm with soil.After straight, transplant the muskmelon seedling.

Result's general introduction, 3 young melons stay smoothly in each district all growth on every young plant of pruning.

Mid-June, every weight in average of the muskmelon during results: test site 920, listing production area 908, no application zone 842(g).

Pol: test site 14.8%, listing production area 14.4%, no application zone 13.4%.

The accumulation time of test site has only 4 days, and listing production area 8 days is all very short, still, when using bacteria preparation, bright mixed fertilizer utmost point in soil became thoroughly decomposed in the short period of time, in addition, do not produce objectionable impurities at the initial stage of becoming thoroughly decomposed yet, therefore, obstacle is not seen in the growth of muskmelon seedling root.

Index: the numerical value at the beginning of 6 months

CEC: test site 10.5, listing production area 9.1, no application zone 6.8(me/100g)

EC: test site 1.8, listing production area 2.2, no application zone 3.2(ms/cm[1: 5]).

Illustrate: though the preparation consumption of test site is only for 50% of listing production area, and is and shorter between accumulational stage, in addition, under the lower condition of growth front-end temperature, an effect of sufficient is sent out in performance.This, with these floras, the soil affinity is strong, the sporulation ability is strong, can stablize under cold condition, character such as breeding is relevant energetically.

Analyze:, can judge the soil improvement progress in each district by the CEC before harvesting time, EC numerical value.Because secular greenhouse cultivation makes the accumulation volume of salt more, therefore, the EC value in 3 districts is all higher, and however, the numerical value of test site is lower than other districts significantly, but EC and CEC numerical value are reflected on the output and pol of muskmelon.

Application examples 4 soil improvement embodiment

Crop; Burdock kind: MITOYO-SHIROHDA

Culture time; Sowing mid-October (fertilizer used after 3 days), late June in next year results (Chiba county)

Cellulosic: straw: test site, the listing equal 300kg/10a in production area, rice hulls: test site: 350, listing production area 350(kg/10a)

Fermentation urea; Test site 0, is provided with preparation in addition and does not have application zone listing production area 4kg/10a.Cultivation is all gone up at sand (clay matter is difficult to form granule less) by the whole district.

Bacteria preparation: the test site, with the bacteria preparation 4 of application examples 2, the listing production area is used and application examples 1 same preparation.Consumption: test site 10, listing production area 20(kg/10a)

Preparation uses: test site preparation 10kg, with the mixture 50kg of rapeseed cake, thick fish meal, bone meal, rice chaff, soya-bean cake, porous matter (diameter 3-30 μ m) green tuff powder 100g, bacterial flora can be bred in the hole of porous ashtuff.The listing production area, amount of formulation increases by 1 times, takes same measure, but, extend to 20 days during the stacking.No application zone same organic fertilizer, stirs at any time and has stacked 3 months.In this test, 3 districts all do not use chemical fertilizer.Above-mentioned cellulosic and organic fertilizer to after ploughing soil and spreading fertilizer over the fields equably, cover 15cm with soil.No application zone, cellulosic there are not input yet.Result's general introduction: listing output: test site 2.46, listing production area 2.34, no application zone 2.15(t/10a).

Index; Numerical value at the beginning of 6 months

CEC: test site 6.4, listing production area 5.2, no application zone 3.6(me/100g)

EC: test site 0.06, listing production area 0.2, no application zone 0.4(ms/cm[1: 53]).

Illustrate: this crop, the cultivation cycle is grown and survives the winter.The stability of bacteria preparation in sandy soil the flora of listing in the production area is not good, dead parts of fine flora in the way, therefore, though used than Duoing 1 times dosage in the test site,, achievement is lower than the test site.

Analyze:, can judge the soil improvement degree in each district by CEC, the EC numerical value of harvesting time.Because sandy soil, the CFC numerical value in 3 districts is all low.However, the numerical value of test site is quite high.This tendency, on the output of burdock also as can be seen.

Application examples 5 soil improvement embodiment

Object crop: rice varieties: HANANO-MAI

Culture time; Rice transplanting the first tenday period of a month in May, results mid-Octobers (chevron county)

Cellulosic: test site, listing production area are straw 580kg/10a

Fermentation urea; The test site production area 4kg/10a that do not have, goes on the market.

Bacteria preparation; The test site, with the bacteria preparation 5 of application examples 2, the preparation same with application examples 1 used in the listing production area.Consumption: test site 10, the M.P.(production area of going on the market) #1:10(four) month uses; The M.P.#2:20(12 month uses) (kg/10a).

In addition, preparation is set and does not have application zone.

Preparation uses: the test site, behind the rice harvest, abandon straw in the field, and spread fertilizer over the fields preparation at the beginning of 12 months, be placed into early April in next year (mid-December first snow, late December accumulated snow, mid-March defrost) by original state.When late April harrows a field and straw bury 10-15cm part in the ground together.Almost at the same time between, use said preparation toward listing among the #2 of production area, implement same processing basically.Use when in addition, 4kg urea harrows a field.In listing production area #1, while administered formulation and urea when harrowing a field.No application zone, straw there are not input yet.

Result's general introduction;

Every strain effective tillering number: test site 22.5, listing production area #1 22.2, listing production area #2 22.4, no application zone 21.0.

Brown rice output: test site 620, listing production area #1 612, listing production area #2615, no application zone 583(kg/10a)

Brown rice thousand seed weight: test site 22.5, listing production area #1 22.2, listing production area #2 22.3, no application zone 21.3(g).

Index; Numerical value at the beginning of 7 months

CEC: test site 30.2, listing production area 28.1, no application zone 23.1(me/100g)

EC: test site 0.2, listing production area 0.3, no application zone 0.5(ms/cm[1: 53]).

Illustrate: because listing production area #2 mainly is made of vegetative cell, expose the part flora that died in the air passing the winter, therefore, use the effect of can not remaining valid of 20kg/10a preparation the December before passing the winter.On the contrary, in the test site, during passing the winter, bacterial flora almost do not have death, therefore, December, the consumption of 10kg/10a was enough, than the 20kg/10a of listing production area #2 or listing at the beginning of 4 months of production area #1 with the using of 10kg/10a, brought into play equal above effect (use the December of phase in slack season, the work branch is mixed favourable).In addition, the preparation that the test site is used contains vinelandii, therefore, compares with the listing production area, does not need input to become thoroughly decomposed and uses urea.

Analyze: though do not measure the arrival clearing time of the floating suspension of paddy field soil,, early August, when coming into each experimental plot barefoot, the test site is softer, and slippery sensation is arranged.Production area #2 is harder in listing, and rough sensation is arranged.No application zone is harder, walks the sensation on the sand.CEC, the EC numerical value in these sensation He Ge districts shows and fully forms granule in the test site.

Application examples 6 soil improvement embodiment (example that the bark compost utilizes)

Crop: tomato kind: Momotaro

Trial period:

Mixed fertilizer is initial:

Test site: early December

Listing production area: early November

No application zone: the early September the year before last

Farming is initial: all in mid-February

Bacteria preparation; The test site, with the bacteria preparation 6 of application examples 2, the listing production area is used and application examples 1 same preparation.

In addition, preparation is set and does not have the district of use.

Use situation; Between accumulational stage for the test site since the first ten days of a month in December, listing production area first ten days of a month in September from the first ten days of a month in November, no application zone from the previous year.3 districts are all since cultivation mid-February (Shizuoka county)

Deciduous tree, acerose bark (part is sneaked into wooden shavings) are 10 tons in collection paper mill, the wood ability factory, pulverize to be several centimeters long.C/N compares and promotion is become thoroughly decomposed in order to adjust, and adds 2 tons of chicken manures, 1 ton in shell fossil powder, 1 ton of rice chaff, 1 ton of residue from beans after making, their is mixed and are adjusted to contain moisture 75%.Each 10kg of preparation, 20kg and their difference thorough mixing are surperficial with plastic film covering (no application zone does not in statu quo cover) then, pile up separately 3 months and 4 months.No application zone is piled up 1 year 6 months (this separates 1 month and ventilates from the bottom surface with air pump).The compost that these barks are made directly drops in the ratio of 3 tons/10a, and the plastic greenhouse during mid-November-early March is heated is ploughed on the soil.Cover 10-15cm with soil then, transplant the luxuriant seedling that adds afterwards at once.

Result's general introduction; Tomato has entered harvesting time from April.In the test site, economical level continues to gather in the crops late November, 11 layers of fruit clump, and the listing production area is the first ten days of a month in November, 10 layers of fruit clump, and no application zone is taked same planting form and period, continuous long-term planting tomato.As a result, in the test site, through also not reducing the output in every year in 7 years.The output in the 6th year of listing production area has reduced by 12% than the mean yield of 1-5, and no application zone the output reduction by 22% in the 5th year, therefore, was replaced other crops on the 6th year.

Index; The numerical value of in mid-May, the 5th

CEC: test site 8.7, listing production area 7.1, no application zone 3.7(me/100g)

EC: test site 0.8, listing production area 1.8, no application zone 3.4(ms/cm[1: 5]).

(the numerical value CEC of in mid-May, the 6th: test site 8.4, listing production area 6.4(me/100g), EC: test site 0.9, listing production area 2.0)

Illustrate: solanaceous crops such as tomato, predispose to damage during farming continuously, generally speaking, even annually drop into a large amount of compost, the continuous cropping more than 5 years is difficult to accomplish.But, as the test site, when correctly using bacteria preparation, can be for a long time on the piece soil farming they and need not change soil in the booth.This is unusual advantageous method.

Each usage quantity of test site preparation only is half of listing production area, and the accumulation time of bark is very short, winter also only 3 months, in addition, the digest process after dropping into soil does not produce the root objectionable impurities, therefore, to the growth of plant without any harm.This to the bark compost produce the dealer, the dealer is favourable in cultivation.

Analyze: from the CEC in the 5th year of continuous cropping, EC value as can be seen, do not have the soil that uses the district, the improvement progress is very poor, the difficulty that tomato continues cultivation occurs.To this, in the test site, though planted continuously 6 years, CEC, EC value representation, soil is very sound, Here it is, the background that continuous cropping is possible.

Application examples 7 soil improvement embodiment (particularly the example of farm crop) all the year round

Crop: pears kind: good fortune water

Culture time: all the year round: test site, listing production area, preparation do not have to execute to use distinguishes 20 pear trees of life in equal 5 years (Ibaraki county)

Cellulosic: around the dried 2-2.5 rice of pear tree, dig the round shape of 60 * 70cm and cheat 4 places,, put in each hole in the test site, be cut into that the long training of 10-15cm trims down branches that 6kg and the bright sawdust of collecting from cowshed and cow dung mix thing 6kg and as the 70gLP nitrogenous fertilizer material of nitrogenous fertilizer.100g urea is executed in listing every hole, production area.

Bacteria preparation; The test site, with the bacteria preparation 4 of application examples 2, the preparation same with application examples 1 used in the listing production area.Preparation is set does not in addition have application zone.Consumption: 150g(1 is used with 1 time in every hole, test site), listing production area 150g(1 is with 2 times), 1 year total consumption: test site: 600g; The listing production area: every of 1200g(),

Behaviour in service; In the test site, late November drops in the cellulosic hole and spreads fertilizer over the fields preparation, suppresses blinding 15cm behind the thorough mixing.The listing production area, in mid-September and mid-March to use with quadrat method with above-mentioned basically.

(but, usefulness be rice hulls 6kg rather than branch).No application zone, cellulosic are also useless.

Result's general introduction;

The output of per 20 listings in the 2nd year: 2.34 tons of test sites, 2.24 tons of production areas of listing, 2.04 tons of no application zone.

Weight in average: test site 29 3g, listing production area 288g, no application zone 274g,

Pol: test site 13.2%, listing production area 13.0%, no application zone 12.3%.

The 2nd year, two districts handled with quadrat method at all the stagger position in hole and preceding 1 year.

Index; The numerical value in July, the 2nd

CEC: test site 9.2, listing production area 8.2, no application zone 5.8(me/100g)

EC: test site 0.3, listing production area 0.4, no application zone 0.8(ms/cm[1: 5]).

Illustrate: test site 1 is only the result who uses 1 preparation and has been surpassed the effect that the production area of going on the market was used 2 preparations in 1 year.

In the listing production area, have any problem as the utilization of cellulosic branch, still,, utilize and also obtained good result as the branch difficult degradation in the test site.This is because the bacterium of using in the test site secretes lipopeptid, Mierocrystalline cellulose enzyme in a large number, and the branch of the difficult decomposition of having degraded powerfully.

Analyze: fruit tree is a property plant all the year round, and the scope of taking root is wide, and therefore, the soil improvement in orchard needs the long years.But, the CEC in the 2nd year, EC numerical value, the soil improvement progress of test site is apparent in view, and this tendency also shows on the output and pol of pears.

The application examples 8 smelly embodiment (fecaluria disappears smelly and cowshed discharge opeing to the harmless example of herbage) that disappears

Object: milk cow (Holstein) herbage (mixed seeding of trefoil, orchardgrass)

Test period; From April in May to next year herbage be perennial (Hokkaido band is wide)

Animal house litters; Test site, listing production area, preparation do not have application zone and all use straw.

Bacteria preparation; The test site is that LISAL C-20(total count is 3 * 10 with the bacteria preparation 2 of application examples 2, the production area of going on the market ⁸/ g, the smelly anerobe preparation of using that disappears, the KK of BIO-LISAL institute goods).Porous matter (the diameter 3-30 μ m) zeolite powder of 9 times of amounts is all added in test site, listing production area to preparation, thorough mixing spreads fertilizer over the fields on littering.

Use situation; The test site, the preparation of above-mentioned processing spreads fertilizer over the fields 400g for every square metre to house floor at every turn, executes 1 time every 8 days.

The same preparation of handling, spread fertilizer over the fields 1 time every 4 days with amount the listing production area.

Result's general introduction; May, use 2 weeks of beginning after, almost the disappeared stench of cowshed of 2 districts.

After 20 days, the ammonia of the position that cowshed central part 1m is high and butanic acid concentration: test site (3 mean value) each 2.5ppm and 0.001ppm, listing production area (3 mean value) 3.5ppm and 0.003ppm, all can not hear stink.No application zone (2 mean value) 17ppm and 0.03ppm.Suitable intensive stench (not feeling stench when ammonia concentration 5ppm is following) is arranged.

After the test site begins 10 days, can not see fly in the cowshed, in the listing production area, disappearance fly after 2 weeks.

Per 1 cow head milk amount of giving milk every year on average: test site, (92 of milk cows) 390kg, listing production area (87) 382kg, no application zone: the 370kg(85 head).

These 2 districts, than no application zone (85) fecund each 5.4%, 4.6%.

Use beginning after 3 months,, observe when flexible pipe is injected in discharge opeing for getting rid of from the drainage tray of cowshed in the test site, float on straw waterborne and all soften, be easy to get rid of draining, still, the difficulty of eliminating work is felt in the 12-3 production area of going on the market between the month.Then very difficult to no application zone.The discharge opeing of these test sites is directly spilt when executing herbage, any bad influence is not seen in the growth of herbage.The listing production area too.In the listing production area, during the 12-3 month, do not smell the smell (not detecting) of hydrogen sulfide.In no application zone, especially, phase in summer stench is very big, and in addition, buoyant straw in the drainage tray has hindered the eliminating drainage works.The draining of no application zone is directly spilt when executing herbage, and part herbage overstrike is delayed growth, recovers normal through 4 months latter.This means that if this draining needs 3 months shelf-time harmless to herbage.

Illustrate: although ratio listing production area, test site, spreading fertilizer over the fields number of times reduces half,, equal above effect appears.This is because in cow dung, the discrepant cause of the reproductivity of bacterium.The test site, the listing production area fly that all disappeared, this point prevents on the viewpoint of livestock products public hazards significant.

Analyze: the listing production area, owing to reduce, postponed the softening progress of straw of drainage tray at the vigor of teletostage bacterial flora, then produced hydrogen sulfide, still, this phenomenon is not observed in the test site.In the increase of the milk yield of test site, reduce with repugnant substance in the cowshed, and it is relevant to alleviate the physiological load of ox.

The application examples 9 smelly embodiment (ight soil disappear smelly and from the harmless compost of chicken manure) that disappears

Object: laying hen (HICH LINE) broccoli (give birth to No. 2 middle morning)

Test period; From May in October to next year, broccoli is planted late May in next year results (outstanding Yu Xian) mid-November

Animal house litters; Test site, listing production area are sawdust, and it is straw that preparation does not have application zone.

Bacteria preparation; The same preparation of application examples 8 is all used in test site, listing production area.Each distinguishes equal 1000 chickens (raise the ground of windowless hen house)

Use situation; From mid-October, per 8 days on the floor of test site and listing production area every square meter spread fertilizer over the fields above-mentioned preparation 500g, spread fertilizer over the fields beginning after 1 month at hen house, sawdust and chicken manure mixture are collected, drop into the field by 2 tons/10a ratio, cover 10-15cm with soil.Plantation broccoli seedling after 4 days.In no application zone, the mixture of stone and chicken manure, the garden taper is piled up on the concrete floor, stirs at any time and deposits 2 months, after the fermentation, by the ratio input field of per 30 tons/10a.Result's general introduction: from mid-October, hen house begins to spread fertilizer over the fields preparation, spends for 2 weeks afterwards in the high 1 meter part of hen house central part, has test ammonia and butanic acid concentration.As a result, each 2.0ppm of test site, nothing detect, listing production area 4.3ppm, 0.0007ppm, and stench has all disappeared.No application zone 7.5ppm, 0.03ppm have felt stench, from (7-12 month laying hen) between 6 months mid-Mays of mid-November to next year average laying rate: test site 85.2%, listing production area 84.9%, no application zone 83.9%.

Average egg weight; Test site 65.2g, listing production area 64.1g, no application zone 63.3g.

The broccoli output of listing: test site 5.84, listing production area 5.61, no application zone 5.12(t/10a)

Broccoli weight in average: test site 1.5, listing production area 1.48, no application zone 1.35kg.

Illustrate: the listing production area, spread fertilizer over the fields 1 times the number of times of Duoing than the test site, still, the result of laying hen, broccoli all is lower than the test site slightly.Reason is to there are differences on the reproductivity of bacterium in chicken manure of preparation.

Analyze: average total egg size (laying rate * egg size) of test site increases by 4.6% than no application zone, this is because the ventilation condition is relatively poor on the windowless hen house structure, and the repugnant substances such as ammonia that chicken manure produces are difficult to get rid of, thus, the chicken of no application zone, be subjected to physiological load (stress).

Application examples 10 feed efficiencies promote embodiment

Animal: beef breed: Dutch ox

Bacteria preparation; The preparation same with application examples 8 used in the test site.Other be provided with preparation do not have to the district.

Test period; From late December to late March.(Wakayama Ken)

The enforcement situation; 1 ton of blankings (moisture 75%) such as the pericarp that produces in citrus fruit juice factory and other byproduct are deposited on the concrete floor, add the diammonium phosphate of preparation 3kg and 0.7%, and after the surface covered with plastic film, original state was stacked 3 days.Afterwards, by weight should product 30% and beef cattle mix with the ratio of mixed feed 70%, to 10 oxen of mean body weight 450kg, blended mixed feed and straw with the method freely absorbed to.Give and the district in nothing, tangerine peel etc. are taked and last same measure, have stacked 6 days.

Result's general introduction; Give with beginning after, every the Niu Tichong that measures two districts for 30 days 1 time.Between 3 months of this duration of test, every day, every the scale of construction that increases was, test site 1.12kg does not have and gives the 1.03kg with the district.Give with 2 weeks of beginning after, the stench that has disappeared and voided excreta in the test site, after 1 month, the stench (nitrogen concentration 4ppm) that the fecaluria of the whole cowshed that disappears produces.Do not have to give and the district stench that the voids excreta (ammonia content: 14-18ppm) that exists.

Feed efficiency; Numerical value between these 3 months (feed, dry converts): test site 15.6%, nothing are given and district 14.3%.

Illustrate: in the test site, form the bacterium of spore, when digester tube (especially in large intestine) passes through, can breed vigorously, thus, can make ight soil disappearance stench.

Analyze; The feed efficiency of test site has not been improved 9% than having with the district.This because, in the test site, lipopeptid and cellulose family are produced bacterium and are worked in digester tube, promote that the digestion of roughage is relevant.

Application examples 11 feed efficiencies promote embodiment (feed add and to the example of crop applying fecaluria compost)

Object: fattening pig (LAND RACE); Onion (celestial platform Huang)

Test period; From June in September to next year, onion late October plantation, mid-June gather in the crops.(city wall county)

The animal house stone; Test site wood shavings, preparation do not have to the district straw.

Bacteria preparation; The test site preparation same with application examples 8.

Use situation; To pig give with the fattening mixed feed, add preparation 0.1%, behind the thorough mixing to.Two districts fatten and go too far after 2 months all for the pig with mean body weight 60kg.

In the test site, this feed give with beginning 1 month after, the mixture of wood shavings and pig manure is collected, the ratio by 2 tons/10a drops into the field at once, afterwards, covers 10-15cm with soil.Planted onion set in 4 days later on.Preparation do not have to the district, pig manure is stacked on the concrete floor, stir at any time, after becoming ripe compost in 2 months, drop into the field by the ratio of 3 tons/10a.

Result's general introduction; Since September to preparation, the stench of the test site that after 2 weeks, almost disappeared (after 1 month, the ammonia concentration 4ppm of pig house).

Every of every day between 2 months on average increase the scale of construction: test site 739g, do not have and give and district 702g.

The onion output of listing: test site 5.88, nothing are given and district 5.27(t/10a)

The onion weight in average: test site 395g, nothing are given and district 345g.

Feed efficiency: test site 25.7%, nothing are given and district 24.4%.

Illustrate: give and the district in nothing, though dropped into the fertilizer of 3 tons/10a,, the output of onion compares low 14% with the test site.The product of test site, balling are very tight, and the percentage of germination in storage obviously reduces, the performance good preservation.In the test site, though use jackleg, as not have composting pig manure,, be not only crop root is not had the infringement of producing, and, promote soil improvement, provide good influence to the growth of crop.

Analyze: the feed efficiency of test site has not been improved 5.3% than having with the district.This effect is produced the digestibility that the vigorous breeding of bacterium causes with lipopeptid and Mierocrystalline cellulose enzyme in the digester tube of pig and is improved, and disappearance pig house stench to alleviate the physiological load of pig relevant.

At first, following description is as the preparation effect of soil improvement.As, application examples 2 and 5 is seen that preparation of the present invention promotes the formation of granule effectively.In case fully form granule, the surface-area of clay particle sharply enlarges, thus, the down leaching of fertilizer composition is reduced on the easier surface (increase of CEC) that absorbs of fertilizer composition, improves the utilising efficiency and the persistence of fertilizer, as a result, can increase economic efficiency.Simultaneously, the fertilizer absorbing power of plant is strengthened, benefit the vigorous growth of plant.

Just, also can obtain the long-term effect of using complete fermentation fertilizer field to be produced continuously with the field of this preparation.This means that the height granule that always has only the outstanding peasant household of minority to obtain forms the result, common peasant also can be at short notice, and laborsaving, province can obtain with taking.We can say to have the useful technology of promotional value.

Shown in application examples 1-5 and 7, preparation of the present invention can overcome the trouble of piling up the cellulosic material and save time.Displaced is before plantation or sowing crop the cellulosic material to be piled up in the concrete facility up to they fully fermentings, then they is mixed in the soil; It is harmless to plant growth, and is then laborsaving economical again.

In addition, preparation of the present invention can be prevented the infringement to crop root that principal vertical line goes out to cause, and this is because nematode fears in the cellulosic material degradation process in anthropogenic soil by bacterium excretory organic acid of the present invention, as formic acid, and acetate, lactic acid.See that with this viewpoint preparation of the present invention can prevent the infringement that causes because of continuous farming.

If the peasant with preparation of the present invention, uses 2-3 together continuously with cellulosic, the locating of 30-40cm under the face of land obviously increases cellulosic fine resolvent (humic etc.), simultaneously a very easy formation.At underground water, because capillary phenomenon by the interstitial diffusion between the granule, after a seizure, is slipped at intragranular than the depths.In other words, the formation of granule is many more, and therefore the moisture holding capacity (this means, provide stable more breeding place to bacterium) that improves soil moisture more runs into prolonged drought and also can keep root moisture on every side, can improve drought-resistant ability.Because the water flowing on every side of granule is good, get rid of superfluous moisture (draining is good) effectively, therefore, run into the damage or crop failure caused by waterlogging that heavy rain also can alleviate crop.Preparation of the present invention provides good condition to the growth of plant.

Air permeability is good around granule, and increases by the fertilizer of a sorption, can reduce the ion (reduction of EC value) of fertilizer composition.These situations, help the level of plant roots, the vigorous growth of vertical direction, and enlarge the root circle, promote the root hair flourishing well, because nutrition is by the easier absorption of soil root, therefore can increase the soil nutrient absorbing power of root, make plant vigorous growth, with this, base of leaf portion also grows well, the rising photosynthetic capacity, the result improves the yield and quality of producing thing.

Because the life-span of preparation of the present invention in soil is longer, when using, can reduce preparation of the present invention and cellulosic access times according to following method, 1 year with once just bringing into play abundant effect, especially under the prerequisite of vinelandii and usefulness.When more difficult dispersive rice hulls, branch, sawdust, wooden shavings and the straw that decomposes easily, remaining cauline leaf, fallen leaves etc. drop into the field together, at the cultivation initial stage, consume the latter as vegetative nutrition source, and then consume the former as vegetative nutrition source.In this process, long-term excretory lipopeptid and deepen cellulosic decomposition by the Mierocrystalline cellulose enzyme of its output.Simultaneously, provide for a long time, the condition of output lipopeptid constantly.Meanwhile, for long during the cultivation, perennial, the also usefulness of the LP nitrogenous fertilizer that specially designs of living crop all the year round can keep long-acting (application examples 7).Object is made in preparation of the present invention is perennial to long evil spirit of cultivation phase, Rhizome and root of Udo, bamboo shoot, fruit tree, tea, mulberry tree etc., the flowers of the grass of living plant, or perennial root all the year round etc., can laborsaving, application effectively.Therefore, not remarkable for the agent effect of last Chinese system of weights, difficult crop of using also can make object of the present invention.

Because the life-span of preparation of the present invention in soil be long, and forms, therefore,, pass the winter and the next spring can both be brought into play positive effect (application 1,4,5,7) in farming late fall or early winter of sudden strain of a muscle phase by the high sporeformer of stability.Owing to can not give full play to the object of effect to the listing preparation good effect is arranged also, therefore mix also very favourable at rural labour's branch.Say that more specifically except that wheat, broccoli, onion, burdock, the staple crop of the open-air cultivation of passing the winter also has crops such as barley, Chinese cabbage, sieve fore-telling, spinach, lettuce lettuce, shallot, potato, Hu Luobu, garlic, pea.To these crops, going into before the winter,, accurately use preparation of the present invention, from the spring to the harvesting time, can give full play to effect according to general use standard.

When using under the condition of preparation of the present invention easy formation granule in soil, can bring into play better effect.But,, in the subtropics or the pineapple in Okinawa of tropical red earth cultivation, sugarcane etc.,, be difficult to form the adaptation place of microbial reproduction because the content of clay or humic is few the ox (application examples 4) of sand cultivation, sweet potato etc.Therefore, when using preparation of the present invention in this field, need take measures in advance the bacterial colonisation of this agent after the hole of porous powders such as silicic acid, slower cellulosic spreads fertilizer over the fields with becoming thoroughly decomposed.Like this, can bring into play abundant effect.

From the rubbish in food or the food-processing, produce stench at digest process, therefore,, be difficult to handle near the resident living area.But, when accurately using preparation of the present invention, at digest process stench takes place hardly, the compost of output thus is not even fermentation produces objectionable impurities (application examples 3) to crop root yet.When preparation of the present invention is suitably used, do not produce stench, therefore, the household garbage that local government collects can be handled in the resident living area.In addition, do not need ventilation between accumulational stage and stir, the peasant can pile up the cellulosic material at short notice, does not need to set up special in addition treating refuse or compost facility, therefore is economical and economizes the labor force's.

When the cellulosic that difficulties such as bark are become thoroughly decomposed is made the raw material production compost, need the heap more than 2 years could get the complete ripeness compost that does not produce objectionable impurities after only usually.If use common composting to promote microbial preparation, also need to ventilate at any time and stir operation, and need the above accumulation time of half a year at least, therefore, the bark compost of general rare good matter.To this, when accurately using preparation of the present invention, can obtain the compost harmless to plant root, and the time of piling up 3-4 month just can use, be very laborsaving, the method that economic benefit is high (application examples 6).The application of this agent can be got rid of, and is at present especially common in the plastic greenhouse cultivation, uses complete ripeness bark compost not and the root injury problem that causes.

Recently, common peasant carries out arable farming with organic fertilizer (or mainly using organic fertilizer).When organic fertilizer is directly used the field, can produce volatile nitrogen composition etc., be subjected to their influence can reduce productivity the root objectionable impurities.Therefore, take usually,, during certain, pile up, decompose to a certain degree back input field as compost production.As, state in the situation of the using place of application examples 4, preparation of the present invention, when using with organic fertilizer, can be unharmful even do not pile up to root yet, because there is not the volatile nitrogen composition.

Unstable situation appears in other anerobe preparations when contacting with air, to this, preparation of the present invention is only by being made up of the spore bacterium, therefore, contact with air or and under other materials coexist, also can keep stability and keeping quality highly.Therefore, can impact effect even before using, preparation of the present invention is mixed with organic fertilizer yet.Many labours can bought and save to the product that comprises dry organic fertilizer and preparation of the present invention on market.In addition, also can take the water-soluble back of a preparation of the present invention to use.

Describe below, on livestock products, use the effect of preparation of the present invention.When correctly using this agent, in animal house, produce stench hardly.We can say that preparation of the present invention prevents that near the livestock products Odour nuisance that produces animal house and the resident living area truly having of being from imitating and useful cheaply preparation (application examples 8,9).In application examples 8, also can see.If preparation of the present invention is used,, therefore can't see fly owing to do not produce volatility repugnant substance such as ammonia in cowshed.

If the animal house that is difficult to topple over or decomposes litters the mixture of material and fecaluria and suitably handles with preparation of the present invention, then can be used as mixed fertilizer.At present, the peasant once attempted they are used as fertilizer, but because they contain unfermentable cellulosic material such as sawdust, wooden shavings etc. (can produce the deleterious material of crop root such as phenol and volatility nitrogenous source) usually, therefore, they can cause the infringement to plant growth.

If certainly, want these raw materials are resolved into the stage that does not produce objectionable impurities fully, also needed to pile up 5-6 month this difficult in practice doing in the warm phase.On the other hand, shown in application examples 9, preparation of the present invention does not only produce objectionable impurities but also can suppress to produce the bacterial reproduction of objectionable impurities, therefore, need not pile up and just can add in the soil, be difficult owing to obtain the high quality compost in the crop farming, therefore, said preparation is very useful.

An amount of when dropping into preparation of the present invention in animal house, the livestock products discharge opeing, can not damage even receive from animal house that the discharge opeing of collection is sprayed onto on the field of firm farming plant-growth yet.This is because lipopeptid and cellulase are secreted in discharge opeing, its quick degradation of rice straw and other cellulosic material.The flowability of discharge opeing has increased and has been easy to and has been decomposed in addition.

Especially, preparation of the present invention by stability strong the spore bacterium form, its can pass the winter highly stablely and when producing stench or discharge opeing problem in generation effect in early spring, therefore, even they are sprayed onto on the animal house during from the late fall to the early spring, also be very effectively (application examples 8).

At last, preparation of the present invention is as the following description of effect of feed effect promotor.

Shown in application examples 10, in the application of preparation of the present invention as feed effect promotor, when it is added in the roughage of multifilament matter, its effect highly significant.In addition, the stench that is produced by animal excrement can suppress by adding said preparation.Shown in application examples 11, when it is added in the mixed feed, can see its effect.It also can reduce and not digest feed and ight soil viscosity.Therefore, it is useful for decomposing fecaluria.

Claims (8)

1, the bacteria preparation of soil improvement, it comprises that can produce the lipopeptid that can reduce water surface tension and have can be under anaerobic, the bacillus that in the soil that sponge exists, breeds, the bacillus or the fusobacterium bacterium that breed can lacked under the anaerobic condition with producing cellulase and having in the soil that sponge exists.

2, according to the bacteria preparation of claim 1, the wherein said bacterium that can produce lipopeptid is selected from subtilis and Bacillus licheniformis-or various bacteria, the described bacterium that can produce cellulase is to be selected from subtilis, Bacillus licheniformis, bacillus polymyxa, one or more bacteriums of Cl.Cellulolyticum and Cl.aerotolerans.

3, the bacteria preparation of soil improvement, it comprises the described bacterium that produces lipopeptid, can be under anaerobic but can produce the bacterium and the fixed nitrogen of cellulase and have, the bacillus or the fusobacterium bacterium that in the soil that sponge exists, breed.

4, according to the soil improvement bacteria preparation of claim 3, the wherein said bacterium that produces lipopeptid is to be selected from subtilis, Bacillus licheniformis, Bacillus circulans, bacillus polymyxa, one or more bacteriums and the described nitrogen-fixing bacteria of Cl.Cellulolyticum and Cl.aerotolerans are to be selected from B.azotofixans, soak numb genus bacillus, one or more bacterium of acetone clostridium butylicum and Pasteur's gemma rib bacterium.

5, the reodorant of animal fecaluria, it comprises described bacterium and the described bacterium that produces cellulase that produces lipopeptid.

6, the reodorant of claim 5, it is wherein said that to produce the lipopeptid bacterium be one or more bacterium that is selected from subtilis and Bacillus licheniformis, the described bacterium that produces cellulase is to be selected from subtilis and Bacillus licheniformis, Bacillus circulans, bacillus polymyxa, one or more bacteriums of Cl.Cellulolyticum and Cl.aerotolerans

7, the feed efficiency promotor of animal-feed, it comprises and describedly produces the lipopeptid bacterium and describedly produce the cellulase bacterium.

8, according to the feed efficiency promotor of claim 7, the bacterium of wherein said generation lipopeptid is one or more bacterium that is selected from subtilis and Bacillus licheniformis, the described bacterium that produces cellulase is to be selected from subtilis, Bacillus licheniformis, Bacillus circulans, bacillus polymyxa, one or more bacteriums of Cl.Cellulolyticum and Cl.aerotolerans