CN1181745C - Lactic-acid-bacteria peptide milk beverage and its preparation technology - Google Patents

Abstract

The present invention relates to a lactobacillus peptide milk beverage and a preparation process thereof. The beverage is prepared from fourteen raw materials comprising fresh milk, white granulated sugar, a stabilizing agent, FH9CMC-Na, water, marrow peptid, placenta peptid, collagen peptid, Danmark lactobacilli, acesulfame potassium, lemon acid, apple acid, citrate sodium and edible essence. The specific recipe and the specific preparation process are disclosed in the specification. The beverage has the advantages of containment of biological active peptide, small molecule, rapid absorption, strong activity and immunity adjusting function in addition to having the characteristics of delicious sweet and sour taste and easy digestion; the beverage has the advantages for promoting the metabolism of cells, balancing the nutrition of cells, and improving the immunity of human bodies. The present invention provides an ideal beverage with the effects for beautifying faces and keeping youthfulness on women. The beverage is suitable for persons who are in good health or subhealth states.

Description

Lactobacillus peptide milk beverage and preparation technology thereof

Technical field

The present invention relates to food required in the human daily life, specifically relate to lactobacillus peptide milk beverage and preparation technology thereof.

Background technology

Development along with protein biochemistry, physiology and nutrition, it is found that, the mankind ingest protein after the effect of digestive enzyme, are not to absorb with amino acid whose form as thinking in the past, more are to absorb with low peptide (as two, tripeptides etc.) form.The absorptivity of low peptide is absorbed by body than amino acid is easier, faster than amino acid height.Some low peptide can not only provide people's bulk-growth, grow required nutriment, and has the effect of preventing and curing diseases, regulating human physiological functions simultaneously, some is the physiological function of former food protein or the unexistent uniqueness of its composition amino acid, and the composition amino acid of many active peptides might not be essential amino acid.Therefore the research and development of biologically active peptide have become the urgent problem of food educational circles.

Summary of the invention

The objective of the invention is to overcome above-mentioned the deficiencies in the prior art part, and provide a kind of raw material sources abundant, lactobacillus peptide milk beverage and preparation technology thereof that immunoloregulation function is good.

Technical solution of the present invention is as follows: lactobacillus peptide milk beverage is to be made by following raw material and prescription by weight, and in 1000 portions of beverages, and water is adjusted to 1000 portions of beverages:

400 parts of fresh milks

60 parts of white granulated sugars

2 parts of stabilizing agent S-3503

2.8 parts of FH9 CMC-Na

Bone marrow peptide 10-12 part

Placenta peptide 0-2 part

Collagen peptide 0-2 part

0.010 part of Denmark lactic acid bacteria

Acesulfame potassium 0.110-0.120 part

0.462 part of citric acid

0.224 part of malic acid

0.346 part of natrium citricum

Flavoring essence 0.035-0.040 part.

The technology of preparation lactobacillus peptide milk beverage, following steps:

1, milk collection

Regain fresh milk from the pasture, go into compensating groove, outgas, filter, cool off, go into milk-storage tank;

2, pure milk gives processing

The pure milk of refrigeration is squeezed into compensating groove, heating, centrifugal clean breast heats up, and cooling adds bacterial classification, capping;

3, fermentation

About 7 hours of ferment at constant temperature is 96-100 ° of T up to acidity, cooling;

4, change glue

In fermentation, change glue, add water, stabilizing agent and granulated sugar is dried be mixed even after, go into tuner, be stirred to stabilizing agent and dissolve;

5, dissolving peptide powder

In fermentation ends preceding 20 minutes, dissolving peptide powder heated up 80 ℃, constant temperature 20 minutes, and pasteurize is cooled to 20 ℃, and is stand-by;

6, allotment

Acidified milk stirs, and goes into blend tank, surveys acidity, is sequentially added into the aqueous solution of acesulfame potassium, citric acid, malic acid and three kinds of mixed acid of natrium citricum and peptide that water mixes up.

7, the homogeneous degassing

With the peptide sour milk beverage of suckling, give heat to 45 ℃, through the degassing, homogeneous, homogenization pressure is 180-200bar;

8, sterilization, can

Through 110 ℃ of (keeping 6 seconds) ultra high temperature sterilizations, cooling, postprocessing working procedures such as packing.

Stabilizing agent S-3503-mixed mode yoghourt stabilizing agent, its composition comprises: high first Yankee pectin, sodium carboxymethylcellulose, xanthans, locust bean gum, phosphate, tamarind seed polysaccharide glue, guar gum.

FH9 CMC-Na: sodium carboxymethylcellulose, acidproof stabilizing agent.

The introduction of three kinds of peptide powder:

1, the bone marrow peptide that extracts from the fresh ox and sheep bone meat tissue

Nourishing peaceful polypeptide is the biologically active peptide that my company extracts from the fresh ox and sheep bone meat tissue, is had " regulating immunity " health care foods by health ministry approval production in 1997.This active peptide product is added in the milk, and the beverage of making has immunoregulatory effect undoubtedly.

2, from the placenta peptide of sheep placenta dish tissue extraction

Placenta has abundant nutrition and special physiological action, and the effective ingredient in the placenta has the function of beauty treatment and extensively approved.My company adopts biotechnology, nutriment in the placenta is separated purifies, and its macromolecular good protein is become active peptide through proteasome degradation, more after filtration, ultrafiltration, concentrate, drying forms the placenta peptide powder.With making beverage in this peptide powder adding milk, has the effect that promotes that cell metabolism, statocyte nutrition, beauty treatment are supported Yan, delayed senility.

3, the collagen peptide that from animal skin, the tendons of beef, mutton or pork, extracts

Animal skin, the tendons of beef, mutton or pork have abundant collagen and elastin laminin, through autoclaving, protein in the tissue is soluble in water, utilize bio protease to be degraded into active peptide, ultrafiltration more after filtration,, concentrate, drying forms collagen peptide powder, this peptide powder is added the beverage of making in the milk, the synthetic good active basic substance that provides of collagen in the cell metabolism procedure in the skin and elastin laminin can be provided.Long-term drinking makes skin bright and clean flexible.

The quality standard of three kinds of peptide powder:

The bone marrow peptide sample is delivered to nutrition and food hygiene teaching and research room of Beijing Medical University, and this teaching and research room has made health food immunoregulation effect survey report, now assay is presented below:

Test rating:

Spleen of mouse and thymus gland are heavy; Antibody forming cell's (PFC) mensuration improves slide method with Jerne; Delayed allergy (DTH) is measured with the pedal swelling method; The detection of macrophage phagocytic function adopts the carbon particle clearance method to measure statistical method: with two groups of mean checks.

Animal used as test:

Kunming kind male and female mouse, age in 6-8 week, 18-22 gram, scientific experiment animal portion of Beijing Medical University provides, and the quality certification number is: the moving word 01-3049 of doctor.

Experimental design:

Animal is divided control group, low dose group, middle dosage group and high dose group.The dosage of above-mentioned three experimental group is respectively 0.25g/Kg, 1.0g/Kg and 3.0g/Kg body weight.Be equivalent to 2.5,10 and 30 times of people's consumption every day respectively, the continuous irrigation stomach is 14 days altogether.

Measurement result:

1, thymic weight of each dosage group mouse and control group more all have tangible increase, and mouse spleen weight and control group are not seen significant change (table 1).

2, middle and high dosage group mouse IgM-PFC number/full spleen and apparent in view increase (table 2) of control group.

3, the delayed allergy of high dose group mouse (DTH) relatively has obvious enhancing with control group, and low, middle dosage group and control group are not seen significant change (table 3).

4, each dosage group mouse carbon particle clearance rate (K) and clean up index (a) and control group and do not see significant change (table 4).

Table 1 bone marrow peptide is to mouse immune organ weight's influence (X ± S)

Group	n	Spleen phase counterweight	Thymus gland phase counterweight
				Solvent control	12	5.82±1.31	3.70 ±0.68
Low dose group	12	5.81±1.56	4.58±1.10*
				Middle dosage group	12	5.71±1.13	4.54 ±1.27*
High dose group	12	5.54±1.16	4.48 ±1.02*

Heavy (the mg)/body weight (g) of phase counterweight=internal organs

Compare with control group ^*P＜0.05

Table 2 bone marrow peptide is to the influence (XS) of mouse IgM-PFC/ spleen

Group	n	The full spleen of IgM-PFC/
			Solvent control	12	91，201 2.24
Low dose group	12	117，490 1.41
			Middle dosage group	10	234，423 1.40 **
High dose group	12	213，796 1.44 **

Compare with control group ^*P＜0.01

Table 3 bone marrow peptide is to the influence of mouse DTH reaction (X ± S)

Group	n	The foot sole of the foot increases thickness (mm)
			Solvent control	12	0.52±0.14
Low dose group	12	0.52±0.17
			Middle dosage group	12	0.63±0.18
High dose group	12	0.66±0.18*

Compare with control group ^*P＜0.05

Table 4 bone marrow peptide is to the influence of mouse carbon particle clearance (X ± S)

Group	n	K	α
				Solvent control	11	0.0436±0.0180	6.80±0.77
Low dose group	11	0.0471±0.0077	6.94±0.62
				Middle dosage group	11	0.0559±0.0202	6.88±0.73
High dose group	12	0.0380±0.0082	6.81±0.49

Brief summary as a result:

Its mouse oral gave bone marrow peptide after 14 days, and testing result shows that bone marrow peptide is significantly increased mouse thymus weight, strengthened the effect of humoral immune function (IgM-PFC) and cellular immune function (DTH), and not seeing significantly influences macrophage phagocytic function.

" the health food function assessment is estimated and the method for inspection " according to the promulgation in 1996 of supervision department of the Ministry of Public Health carries out drosophila survival is tested in the delaying senility function detection and evaluation, now placenta peptide detected and estimates.

Animal used as test and tried thing: Drosophila melanogaster (Drosophila melanogaster).Breed according to conventional method, collect the adult that sprouts wings in 10 hours, etherization is liked, distinguishes male and female.Contain international (Shanghai/Zhangjiakou) bioengineering Co., Ltd by three batch number is provided: 970418.

Reagent and instrument: dark incubator, cultivation vial, ether, benzoic acid, beautiful material powder, brown sugar, dusty yeast, agar etc.

Animal divides into groups and is tried thing to give: animal used as test is divided into normal control (NC), low dosage (LD), middle dosage (MD) and 4 groups of high dose (HD) at random, about 100 of every group of female male drosophila, placenta peptide is added in the fruit bat basal feed of fusing, fully mix well, LD, MD and HD group are raised with the fruit bat basal feed that contains 0.2,1.0 and 5.0% placenta peptide respectively, the NC group is raised with normal diet, lasts till that experiment finishes.

Experimental procedure and method: per 25 culture tubes of weighing and be placed on of animal used as test (in 3 * 13cm), are raised in 28 ± 1 ℃, the dark incubator of relative humidity about 55%.Culture medium thickness is 0.5-1.0cm in every culture tube.Changed a subculture in every 3-4 days, regularly add up fruit bat death toll and the eliminating fruit bat because of other reasons death every day for 3 times, up to whole death.After the off-test, calculate maximum life span, average life span and half death time, maximum life span is the average of 10 fruit bat adults survival fates of every group of last death, average life span is pressed calculated with weighted average method with the death toll of difference time-to-live fruit bat, and the half death time tries to achieve with the cumulative mortality and the return law of the straight line of survival fate.

The result

Placenta peptide is to the influence of fruit bat average life span:

As can be seen from Table 1, placenta peptide low, middle dosage does not have the prolongation effect to the fruit bat average life span, and the placenta peptide of high dose is to the equal significant prolongation of fruit bat average life span.

Table 1, placenta peptide are to the influence of fruit bat average life span

Group	Sex	Sample number	Average weight (μ g)	Average life span (d) n	The P value
						Control group	Male	95	780	30.2±9.2
	Female	97	920	32.7±9.0
						Low dose group	Male	82	780	30.8±11.6	＞0.05
	Female	93	920	32.6±12.6	＞0.05
						Middle dosage group	Male	83	780	32.1±10.7	＞0.05
	Female	103	920	34.6±10.7	＞0.05
						High dose group	Male	93	780	34.0±9.7	＜0.05
	Female	96	920	35.4±10.0	＜0.05

a：X±SD

2, placenta peptide is to the influence of fruit bat half death time:

From table 2 can, the placenta peptide of low dose group is to the not prolongation effect of half death time of fruit bat, the placenta peptide of middle dosage is with female male drosophila half death time significant prolongation, the high dose placenta peptide is to female equal significant prolongation of male drosophila half death time.

Table 2, placenta peptide are to the influence of fruit bat half death time

Group	Sex	Sample number	Average weight (μ g)	Average life span (d) n	The P value
						Control group	Male	95	780	29.0±10.3
	Female	97	920	31.2±9.0
						Low dose group	Male	82	780	29.3±10.9	＞0.05
	Female	93	920	32.0±11.3	＞0.05
						Middle dosage group	Male	83	780	31.1±9.8	＞0.05
	Female	103	920	33.8±9.0	＜0.05
						High dose group	Male	93	780	32.7±10.9	＜0.05
	Female	96	920	34.2±12.0	＜0.01

a：X±SD

3, placenta peptide is to the influence of fruit bat maximum life span:

As can be seen from Table 3: the placenta peptide of low dosage does not have the prolongation effect to the maximum life span of fruit bat, in and the placenta peptide of high dose the maximum life span of female fruit bat is all had significant prolongation.

Table 3 placenta peptide is to the influence of fruit bat maximum life span

Group	Sex	Sample number	Average weight (μ g)	Average life span (d) n	The P value
						Control group	Male	95	780	44.8±2.7(n＝10)
	Female	97	920	47.6±2.1(n＝10)
						Low dose group	Male	82	780	44.9±2.9(n＝10)	＞0.05
	Female	93	920	47.4±3.9(n＝10)	＞0.05
						Middle dosage group	Male	83	780	45.8±2.0(n＝10)	＞0.05
	Female	103	920	49.6±2.0(n＝10)	＜0.05
						High dose group	Male	93	780	46.5±2.0(n＝10)	＞0.05
	Female	96	920	52.2±2.9(n＝10)	＜0.01

a：X±SD

Estimate:

From the result of above three indexs as can be seen, the placenta peptide of institute's amount of reagent all has more than one dosage that the prolongation of life span of drosophila melanogaster is had positive effect, can judge that the drosophila survival result of the test of placenta peptide is positive.

" the health food function assessment is estimated and the method for inspection " according to the promulgation in 1996 of supervision department of the Ministry of Public Health utilizes D-galactolipin aging model animal to carry out the detection and the evaluation of mouse lipid peroxide content and aging relevant enzyme, placenta peptide is detected and estimates, and its result is as follows:

1, animal:

Kunming kind female mice about 6 ages in week, weight range 18-22g is available from the PLA General Hospital Experimental Animal Center.

2, reagent:

Analyze pure D-galactolipin, thiobarbituricacid (TBA), available from Shanghai reagent two factories, tetraethoxypropane (TEP) is the Difico packing, available from chemical reagent shop, Beijing, folded ammonia sodium, reduced glutathione available from Huamei Bio-Engrg Co.,, two sulphur paranitrobenzoic acids (DTNB), available from Beijing hundred safe Biochem Technology, INC., other general reagent is all purchased medicines and appliances storehouse, the White Army thing Academy of Medical Sciences.

3, animal grouping and administration:

Animal is divided into normal control (NC), D-galactolipin model contrast (DC), placenta peptide low dosage (LD), middle dosage (MD) and 5 groups of high dose (HD) at random.Every group of 10 animals.Hypodermic injection physiological saline 0.2ml behind NC and the DC group difference every day neck, hypodermic injection D-galactolipin aqueous solution 0.2ml (60mg/kg body weight) behind DC, LD, MD and HD group neck every day, LD, MD and HD treated animal gavage placenta peptide content suspension every day, and dosage is respectively 20.8 (containing selenium 8.33 μ g) and 125.0 (containing selenium 25.0 μ g) prosperous pulvis/kg of mg selenium.Be equivalent to oral minimum recommended dosage 1 capsules of normal adult day, promptly 5,10 and 30 times of 250mg (containing selenium 50 μ g) 60/kg body weight, continue to live after 30 days and kill.

4, observation index and method:

MDA content in the mouse whole blood adopts the TBA fluorescence method; GSH-PX vigor in the mouse whole blood adopts the GSH-DTNB colorimetric method.

5, statistical disposition: all data are carried out variance analysis (ANOVA) with SAS software

The result

1, placenta peptide is to the influence of MDA content in the mouse whole blood:

From result shown in the table 1 as can be seen, the placenta peptide of comparing many amount of reagent with control group all can obviously reduce the lipid peroxide content of mouse.

Table 1, placenta peptide are to the influence of MDA content in the mouse whole blood

Group	Dosage (mg/kg)	Mouse number (only)	MDA (nmol/ml whole blood) n
				Model control group	0	12	3.63±0.17
The normal control group	0	11	3.80±0.15*
				Low dose group	20.8	11	3.63±0.082##
Middle dosage group	41.7	9	3.45±0.15*##
				High dose group	125.0	11	3.40±0.057**##

#: compare .P＜0.05:##:P＜0.01 with the DC group

*: compare .P＜0.05:**:P＜0.01 with the NC group

a：x±SD

2, placenta peptide is to the influence of mouse whole blood GSH-PX vigor:

The result shows shown in the table 2, the placenta peptide of the institute's amount of reagent GSH-PX vigor in the mouse whole blood that all can raise, and wherein, middle and high dosage group is compared remarkable rising with control group.

Table 2, placenta peptide are to the influence of mouse whole blood GSH-PX vigor

Group	Dosage (mg/kg)	Mouse number (only)	MDA (nmol/ml whole blood) n
				Model control group	0	12	41.2±3.27
The normal control group	0	11	33.1±3.74**
				Low dose group	20.8	11	35.6±4.70**
Middle dosage group	41.7	9	39.2±4.29##
				High dose group	125.0	11	42.0±3.78##

#: compare P＜0.05:##:P＜0.01 with the DC group

*: compare P＜0.05:##:P＜0.01 with the NC group

Brief summary:

This testing result shows that the placenta Toplink significantly reduces the content of MDA in the mouse whole blood, and therefore the middle and high dosage GSH-PX vigor in the mouse whole blood that can obviously raise, can judge that placenta peptide has delaying senility function.

The present invention's milk compared to existing technology has following advantage:

These product are sour-sweet tasty and refreshing except that having the ordinary lactic acid bacteria beverage, and outside the digestible characteristics, its distinctive feature is to contain biologically active peptide, and molecule is little, absorption is fast, activity is strong.Bone marrow peptide (nourishing peaceful peptide) has immunoloregulation function; Placenta peptide is a kind of cosmetology function factor of uniqueness; The collagen peptide is the good active basic substance of collagen and elastin laminin in the synthesized human skin.So children, the normal drink of adult this product can promote cells in vivo metabolism, statocyte nutrition, improve immunity of organisms.Lady type lactobacillus peptide milk beverage makes skin obtain from inside to outside nourishing effect, is modern lady's beauty, the desirable drink of supporting Yan.

The specific embodiment

Enumerate three embodiment below, the present invention is further specified.

Embodiment 1: the child form beverage

It is as follows to fill a prescription: the raw material kilogram

Fresh milk 400

White granulated sugar 60

Stabilizing agent 2

FH9CMC-Na 2.8

Bone marrow peptide 10

Denmark lactic acid bacteria 0.010

Acesulfame potassium 0.120

Citric acid 0.462

Malic acid 0.224

Natrium citricum 0.346

Flavoring essence 0.035

Water 524.003

With the fresh milk that regain in the pasture, pour compensating groove into, through outgasing, filtering, be cooled to 0-4 ℃, squeeze into milk-storage tank, refrigeration;

Pure milk give processing,

The plain chocolate of refrigeration is squeezed into compensating groove, be heated to 35-40 ℃ by plate type heat exchanger, centrifugal clean breast is squeezed into fermentation tank, is warming up to 85 ℃ of (keeping 30 minutes) pasteurizes, is cooled to 36 ℃ then, adds bacterial classification, capping immediately;

Fermentation

Capping immediately behind the adding bacterial classification, about 7 hours of ferment at constant temperature is 96-100 ° of T up to acidity, is cooled to 20 ℃, stops fermentation;

Change glue

In fermentation, change glue, add 60 ℃ of warm water of 2/3rds Total Waters in the blend tank, accurate weighing stabilizing agent, with granulated sugar dried be mixed even after, be spilled into blend tank, start stirring always, dissolve fully up to stabilizing agent;

Dissolving peptide powder

In fermentation ends preceding 20 minutes, dissolving peptide powder, earlier with 60 ℃ of warm water dissolvings of 4 letter peptide powder amounts, 80 ℃ of the peptide liquid intensifications after the dissolving, constant temperature 20 minutes, pasteurize is cooled to 20 ℃ afterwards, and is stand-by;

Allotment

Acidified milk stirs, and goes into blend tank, (its acidity is accurately measured in sampling simultaneously, calculates citric acid and malic acid and natrium citricum amount then).Acesulfame potassium dissolves with low amounts of water, and mixed acid is with 20 times of water-soluble separating, and order adds the aqueous solution of acesulfame potassium, mixed acid and peptide then, adds the water to constant at last;

The homogeneous degassing

With the peptide sour milk beverage of suckling, give heat to 45 ℃, through the degassing, homogeneous, homogenization pressure is 180-200bar;

Sterilization, can

Sterile filling: homogeneous lactobacillus peptide milk beverage through 110 ℃ of (keeping 6 seconds) ultra high temperature sterilizations, is cooled to 25 ℃, sterile filling, vanning, distribution again;

Bottled: without superhigh temperature, homogeneous lactobacillus peptide milk beverage is directly squeezed into another blend tank, is warming up to 80 ℃, and canned, 88 ℃ of re-pasteurization stills (protecting 20 minutes) sterilization is advanced in capping again, cooling, decals, vanning, distribution.

Product of the present invention mainly is applicable to the crowd of health, inferior health.

Claims (2)

1, lactobacillus peptide milk beverage is characterized in that it is to be made by following raw material and prescription by weight, and in 1000 portions of beverages, and water is adjusted to 1000 portions of beverages:

400 parts of fresh milks

60 parts of white granulated sugars

2 parts of stabilizing agent S-3503

2.8 parts of FH9 CMC-Na

Collagen peptide 0-2 part

Bone marrow peptide 10-12 part

Placenta peptide 0-2 part

0.010 part of Denmark lactic acid bacteria

Acesulfame potassium 0.110-0.120 part

0.462 part of citric acid

0.224 part of malic acid

0.346 part of natrium citricum

Flavoring essence 0.035-0.040 part;

Preliminary treatment, fermentation, change glue, dissolving peptide powder, allotment, the homogeneous degassing, sterilization, can through milk collection, pure milk make this product.

2, the technology of preparation lactobacillus peptide milk as claimed in claim 1 beverage is characterized in that following steps:

(1) milk collection

Regain fresh milk from the pasture, go into compensating groove, outgas, filter, cool off, go into milk-storage tank;

(2) pure milk gives processing

The pure milk of refrigeration is squeezed into compensating groove, heating, centrifugal clean breast heats up, and cooling adds bacterial classification, capping;

(3) fermentation

About 7 hours of ferment at constant temperature is 96-100 ° of T up to acidity, cooling;

(4) change glue

In fermentation, change glue, add water, stabilizing agent, with granulated sugar dried be mixed even after, go into tuner, be stirred to the stabilizing agent dissolving;

(5) dissolving peptide powder

In fermentation ends preceding 20 minutes, dissolving peptide powder heated up 80 ℃, constant temperature 20 minutes, and pasteurize is cooled to 20 ℃, and is stand-by;

(6) allotment

Acidified milk stirs, and goes into blend tank, surveys acidity, is sequentially added into the aqueous solution of acesulfame potassium, citric acid, malic acid and three kinds of mixed acid of natrium citricum and peptide that water mixes up;

(7) the homogeneous degassing

With the peptide sour milk beverage of suckling, give heat to 45 ℃, through the degassing, homogeneous, homogenization pressure is 180-200bar;

(8) sterilization, can

Keep 6 seconds ultra high temperature sterilizations, cooling, postprocessing working procedures such as packing through 110 ℃.