CN109619245A - A kind of method that instant active double protein exempts from digesting protein for raw material preparation solid-state - Google Patents
A kind of method that instant active double protein exempts from digesting protein for raw material preparation solid-state Download PDFInfo
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- CN109619245A CN109619245A CN201811547058.2A CN201811547058A CN109619245A CN 109619245 A CN109619245 A CN 109619245A CN 201811547058 A CN201811547058 A CN 201811547058A CN 109619245 A CN109619245 A CN 109619245A
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G1/00—Cocoa; Cocoa products, e.g. chocolate; Substitutes therefor
- A23G1/30—Cocoa products, e.g. chocolate; Substitutes therefor
- A23G1/32—Cocoa products, e.g. chocolate; Substitutes therefor characterised by the composition containing organic or inorganic compounds
- A23G1/44—Cocoa products, e.g. chocolate; Substitutes therefor characterised by the composition containing organic or inorganic compounds containing peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
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- General Chemical & Material Sciences (AREA)
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Abstract
It is that digestible protein chocolate is exempted from carrier preparation with cocoa butter liquid Guyuan reason that the invention discloses a kind of using cocoa slurry, solves the problems, such as peptide poor taste and fast food.Using fertilized eggs egg through hatching live embryo albumen and soybean protein as raw material, living body embryo mixes with soybean protein to be digested through ultra-long time, and the concentrated spray drying of digesting protein liquid form product of exempting from for obtaining double protein obtains the cooling finished product of the Hybrid Heatings molding such as powder product and cocoa slurry cocoa butter.The product locating students release brainfag and the pressure effect of improving learning efficiency, and fatigue is that the human body pressure closely related with body and mind with spirit is reacted, and most important elimination fatigue is quick supplement protein, and exempting from digestible protein is that unique most quick supplement does not have to digestion.Brain fag reflects that index is oxygenated haemoglobin, and when brain fag is that the index will increase, making us thought slowness learning efficiency is reduced.When testing edible peptide study, oxygenated haemoglobin is reduced, it inhibits to increase, and enables brain awake, resourceful.
Description
Technical field
The present invention relates to a kind of biological food of enzyme engineering technology production, specifically a kind of instant active double protein is
Raw material preparation solid-state exempts from the class chocolate method that digesting protein is mixed with cocoa liq-uor cocoa butter.Exempting from digesting protein is by dipeptides
Tri-peptide molecule amount is formed in the compound amino acid of 300DA to 500DA and average molecular weight in 120DA.
Background technique
Amino acid is the basic unit of constitutive protein matter, and guarantees one of the important substance of human homergy.Human body
The amino acid that can be digested and assimilated only has 20 kinds.Wherein having 9 kinds of amino acid (baby is 10 kinds) cannot be synthesized or close in adult body
The needs of body it are not able to satisfy at speed, referred to as necessary amino acid, it must be supplemented from diet.Scientist thought only have in the past
Amino acid can be absorbed by organisms utilization by small intestine, mention so developing and exempting from the nutritional supplements of the various amino acid of digesting protein
It supplies patient to use, and most of is that the product that biotechnology synthesizes is expensive.But scientist has found small point with the development of science and technology
Sub- dipeptides tripeptides can also be directly absorbed and utilized by small intestine.This invention is exactly to be provided for society with biological enzymolysis technology containing small point
Sub- activity dipeptides, tripeptides and compound amino acid, also exempt from digesting protein product containing embryo viability.
The existing amino acid products for exempting from digesting protein, the obtained amino acid of synthesis generally produced with chemical synthesis is all
It is DL type amino acid, at high cost, reaction is complicated, and step is more, and by-product is more.The many amino acid whole quilts of other methods such as acid-base method
It destroys, and generates racemization.Fermentation method equipment investment is big, and production concentration is low, production cycle long uncomfortable industrialization.
Goal of the invention
Newest human senility theory thinks human body due to aging, and human body causes human body using endogenous protein deficiency
It is to cause one of body aging main cause that internal nitrogen source is unbalance.The daily small intestine of normal human will fall off 250 grams or so of epithelial cell
Albumen, the epithelial cell worked hard averagely only have two day service life, and newborn cell generates its removal of bottom of old cell simultaneously
Old cell is substituted, these aged cells are digested as food, but it is through gastric acid denaturation treatment, protein molecular spiral winding
, protease does not reach the surface of protein, so this part cell protein of normal person digests just for a long time in small intestine lower end
It can be absorbed by the human body.Since aging digestive function reduces, digestive ferment decreased, and activity also reduces, and makes that the elderly's is interior
Property cell protein in source cannot be recycled sufficiently, and daily albumen more than half jin, which cannot supplement, causes nitrogen source unbalance, this is new
It was found that one of the elderly's aging main cause.At this moment you eat how much protein since degenerate your digestive function of body reduces, and obtain not
To digestion just let alone absorb.What if, for the elderly of aging, to provide good digesting protein product of exempting from be unique
The method for solving this health problem.
Fatigue refers to from the human body to spirit and the concentrated expression of the closely related a variety of pressure of our bodies and minds.Fatigue can be seperated
Power fatigue and mental fatigue, for how eliminating fatigue, mental fatigue and physical fatigue are the same.Fatigue is eliminated rapidly,
Protein is most importantly quickly supplemented, exempting from digesting protein is unique first choice, it does not have to digestion and directly absorbs.
Scientific experimentation 1: the reflection index of influence of the soybean peptide to brain activity, brain fag is oxygenated haemoglobin.Greatly
The concentration of brainfag, oxygenated haemoglobin will increase, and make us thought slowness, and work and study efficiency reduces.Test is not eaten
The concentration of oxygenated haemoglobin before study than increasing when soybean peptide learns: and after human consumption soybean peptide, compared with before study, oxidation
Hemoglobin reduces, this proof, soybean peptide inhibits the raising of oxygenated haemoglobin concentration.Test proves: soybean peptide can
Inhibit the concentration of oxygenated haemoglobin to stablize in a certain range, enables brain awake, it is resourceful, it improves learning efficiency.Attached drawing 1
Soybean peptide eliminates brainfag comparison diagram.
Scientific experimentation 2: soybean peptide mitigates the effect of stress, and the reflection index of stress is cortin on kidney.For
For determining soybean peptide to mitigating stress role, when study, takes soybean peptide comparison, human consumption soybean peptide when study, kidney
The concentration of adrenal cortex hormone is considerably lower.Therefore, the human consumption soybean peptide in work or study, being capable of substantially reduced spirit pressure
Power enables brain light, the concentration of attention when keeping learning.The effect picture of 2 soybean peptide of attached drawing mitigation stress.
Peptide is a revolutionary product, it will lead 21 century human nutrition revolution.It undergoes in total since the dawn of human civilization
Health revolution three times is crossed, first time smallpox occurs, and solution is vaccination, and second with mould extract for treating due to viral thin
Microbial disease, third time are exactly all roots for utilizing small active peptides to treat including all major diseases, that is,
Cell failure problems.
Scientist has found a kind of drug that can promote longevity, and is tested with small white mouse and obtained for the first time
Success.The drug of this mystery is exactly the mixture scientific name being made of leucine, isoleucine and valine these three amino acid
These three amino acid are added in the water that mouse drinks in " branched-chain amino acid " experiment, as a result, it has been found that their service life averagely reaches
869 days, and common small white mouse can only then survive about 774 days.That is, the life that " branched-chain amino acid " is small white mouse prolongs
12% has been grown while extending the service life, the biological respinse of series of active also has occurred in the body of small white mouse, as cell is able to
Absorb more energy, free radical reduction etc..Free radical is the hazardous compound generated in body oxidation reaction, has Strong oxdiative
Property, the tissue and cell of body can be damaged, and then cause chronic disease and aging effect.It can prove that this three kinds of amino acid can be with
The intracorporal free radical of animal is removed, by these variation in vivo, small white mouse seems dynamic, and Coordinating Muscle ability mentions
Gao Liao, life extend.It is a kind of particularly significant and effective nutritional supplement that researcher, which introduces " branched-chain amino acid ", it can be with
It helps organism to enhance muscle without any side effect naturally and obtains more energy, remove interior free yl effect.?
Invention amino acid supplements on the basis of this have very great help to the elderly or patient then it can promote the well-being of mankind in future.
Do not find this invention also currently on the market exempts from digesting protein product, which is by molecular weight in 300DA
Compound amino acid in 120DA of small molecule dipeptides, tripeptides and average molecular weight to 500DA (dalton) is formed.Upper interview
Having arrived small active peptides is a revolutionary product, treats all including all major diseases using small active peptides
Root, that is, cell failure problems.How that mends peptide, and there are two types of one, edible small molecule dipeptides and tripeptides for mode.Two, it is
Peptide is squeezed into body by injecting method.Calculating dipeptides by 20 kinds of amino acid has 400 kinds, and tripeptides has to be eaten in 8000 kinds of combinatorial theorys
There are 8400 kinds of functions and effect with small active peptides.Country it is raw all can to press soybean peptide standard without the standard of food peptide now
Food peptide is produced, product can only absorb a little, and science, which has proven to human body, can only absorb the peptide of molecular weight once in 500DA.Soybean
The standard molecular weight of one superfine product of peptide is less than 2000DA and is greater than 80%, and there are also the generations of limiting amino acid, this is highly difficult.This hair
Bright difference be not limiting amino acid generation important molecule amount be less than 500DA be exactly to exempt from digesting protein product.Liberation army army
Bioengineering Research Institute, thing medical college professor Tang Guoying preside over complete soybean peptide imitate in vitro human stomach, trypsase experiment and
Foreign literature reports that result is completely the same, and this kind of product enters body cannot be digested substantially, and peptide surface produces a kind of protection
Film is no longer by enzyme effect.
The technical solution of invention is to use the fertilized eggs of egg to be hatched as live embryo and soybean protein double protein to be raw material, use
Biological enzymolysis technology reaches while saving embryo viability substance and low cost, containing small molecule active dipeptides tripeptides and again
That closes amino acid exempts from digesting protein product.Scientific experiment has proven to the active matter that living body embryonated egg body has a lot of life conversions
Matter is significant to human health.
Summary of the invention
Exempt from digesting protein production it is an object of the present invention to provide the high embryo viability albumen of fraction, based on soybean protein
Product.Predominantly student market provides the protein product quickly absorbed for eliminating fatigue with tension pressure.Raw material fertilized eggs egg
Embryo viability biology.This method be not required in enzymolysis process be afraid of amino acid generation, excessive enzymatic hydrolysis be existing peptide enterprise all
The thing of fearness controls bad product and soybean peptide national standard is just not achieved.The most fundamental problem is that human body can only absorb molecule
Measure 300DA-500DA dipeptides tripeptides.This method is not required to adjust pH value in enzymolysis process, and processing step is simple, at low cost, remain
Bioactive ingredients keep its nutrition, biological value high.
New human senility theory thinks to make due to aged human body insufficient using own endogenous albumen, causes human body
Nitrogen source is unbalance to cause body aging.The daily small intestine of the normal human 8 ounce epithelial cell albumen that will fall off have 249 grams, diligent
The epithelial cell of work averagely only has two day service life, and newborn cell results from the bottom of each villus, its removal and replacement
Old cell, these aged cells are digested as food, but it is not through gastric acid denaturation treatment, so this part of normal person is thin
Born of the same parents' albumen is digested for a long time in small intestine lower end, and be just absorbed by the body utilization.Human digestive function reduces due to aged, disappears
Change enzyme reducing, activity is also reducing.Recycle old man's endogenous cellular protein cannot sufficiently, it is daily excellent more than half jin
Matter albumen cannot supplement, and cause protein insufficient, this is one of the elderly's aging main cause.At this moment no matter you mend albumen more again
Matter is not all used, and cannot be digested just let alone be absorbed.What if, it is unique for providing good digesting protein product of exempting from
The method for solving this health problem.It is also the most important significance of this product exploitation.
Step of preparation process:
1., by taking fertilized eggs as an example, fertilization hatching egg is through artificial incubation (use incubator) Constant temperature hatch method, incubation temperature are as follows:
Hatch at a temperature of 37.8, fertile egg is made to be changed into chicken son's tire albumen mixing soya beans albumen living as double protein raw material, containing height
Active high nutrition ingredient；It is primary with candler candling through hatching the 5th day, remove no fertilized eggs；
2., pearl living will be hatched in 1 to except before young, using embryo's living body biological, to egg shell between hatching 17 to 19 days
Living body is removed after disinfection treatment, adding water to size mixing after low-temperature grinding processing makes it containing solid content between 12% to 15%；
3., with a small amount of disinfectant mixing compound protease, i.e., allotment liquid of protease, compound enzyme dosage are 0.3% -0.5%；
4., step 2 mixed into step 3 heating, temperature control between 54 DEG C to 56 DEG C, which achieves enzymatic hydrolysis simultaneously at a slow speed
Stirring, enzymolysis time are between 68 to 72 hours, and entire enzymolysis process is not required to adjust pH value；
5., the feed liquid after the completion of step 4 enzymatic hydrolysis is carried out to the lysate of the isolated pure small peptide of seperator and compound amino acid；
6., step 5 is carried out to sterilization and destroy the enzyme treatment, feed liquid reaches 75 DEG C -90 DEG C in temperature through coil sterilizer, heat preservation 15
- 16 seconds second, reach sterilising and enzyme inactivating purpose；
7., by step 6 by double effect evaporator concentration material 38% --- after between 43%, be spray-dried to obtain and exempt to digest
Small-molecular peptides (containing compound amino acid) protein product.It obtains 26kg and exempts from digesting protein powder, add 20kg cocoa liquor, add 16kg can
Can rouge, add 15kg granulated sugar.
With steamer discharge water heating then equipped with exempt from digestible protein powder, cocoa liquor, cocoa butter, white granulated sugar container be put into
On steamer all waters surface.Side heating is kept stirring, until container object in the pasty state, can be helped adjust sticky with a small amount of milk, reached
Spoon can descend drop, but not quickly, pour into mold, natural cooling, demoulding packaging after mixing evenly.
The present invention has the advantages that:
The present invention provides the good protein product for really exempting from digestion for society.And provide the dipeptides for exempting to digest small molecule active
Kyrine product, science have confirmed that edible small-molecular peptides, only dipeptides, tripeptides just exempt to digest, and directly pass through intestinal mucosa and enter
Body.
Hereby doctor says famous American biologist carat: small-molecular peptides therapy will be improved at least human longevity 20 years.The U.S.
World traditional medicine academy of sciences doctor, the Committee of Experts, Chinese traditional Chinese medical science great master association director, professor Li Chengsen introduce: having in the world
Eight scientists study a kind of substance peptide and obtain the Nobel Prize, modern science forefront, adjustment human body birth and old age, sickness and death it is most heavy
Substance is wanted, if there will be no life presence without peptide.
Peptide is a revolutionary product, it will lead 21 century human nutrition revolution.It undergoes in total since the dawn of human civilization
Health revolution three times is crossed, first time smallpox occurs, and solution is vaccination, and second with mould extract for treating due to viral thin
Microbial disease, third time are exactly all roots for utilizing small active peptides to treat including all major diseases, that is,
Cell failure problems.
The present invention also provides the compound amino acid protein products for exempting from digestion.Wherein contain strong body in compound amino acid
" branched-chain amino acid " produces " branched-chain amino acid " fluid infusion, will promote the well-being of mankind in future！
Scientist has found a kind of drug that can promote longevity, and is tested with small white mouse and obtained into for the first time
Function.The drug of this mystery is exactly the mixture scientific name " branch being made of leucine, isoleucine and valine these three amino acid
These three amino acid are added in the water that mouse drinks in chain amino acid " experiment, as a result, it has been found that their service life has averagely reached 869
It, and common small white mouse can only then survive about 774 days.That is, the life that " branched-chain amino acid " is small white mouse extends
12% while extending the service life, the biological respinse of series of active also has occurred in the body of small white mouse, as cell is taken the photograph
Take more energy, free radical reduction etc..Free radical is the hazardous compound generated in body oxidation reaction, has Strong oxdiative
Property, the tissue and cell of body can be damaged, and then cause chronic disease and aging effect.It can prove that this three kinds of amino acid can be with
The intracorporal free radical of animal is removed, by these variation in vivo, small white mouse seems dynamic, and Coordinating Muscle ability mentions
Gao Liao, life extend.It is a kind of particularly significant and effective nutritional supplement that researcher, which introduces " branched-chain amino acid ", it can be with
It helps organism to enhance muscle without any side effect naturally and obtains more energy, remove interior free yl effect.This
The left Ni Suoli professor of project researcher's Milan, ITA university grace, he points out invention amino acid supplements on this basis, then it
It can promote the well-being of mankind in future, have very great help to the elderly or patient.
New human senility theory thinks to make due to aged human body insufficient using own endogenous albumen, causes human body
Nitrogen source is unbalance to cause body aging.The daily small intestine of the normal human 8 ounce epithelial cell albumen that will fall off have 249 grams, diligent
The epithelial cell of work averagely only has two day service life, and newborn cell results from the bottom of each villus, its removal and replacement
Old cell, these aged cells are digested as food, but it is not through gastric acid denaturation treatment, so this part of normal person is thin
Born of the same parents' albumen is digested for a long time in small intestine lower end, and be just absorbed by the body utilization.Human digestive function reduces due to aged, disappears
Change enzyme reducing, activity is also reducing.Recycle old man's endogenous cellular protein cannot sufficiently, it is daily excellent more than half jin
Matter albumen cannot supplement, and cause protein insufficient, this is one of the elderly's aging main cause.At this moment no matter you mend albumen more again
Matter is not all used, and cannot be digested just let alone be absorbed.What if, it is unique for providing good digesting protein product of exempting from
The method for solving this health problem.It is also the most important significance of this product exploitation.
Detailed description of the invention
1 soybean peptide of attached drawing eliminates brainfag comparison diagram.
The effect picture of 2 soybean peptide of attached drawing mitigation stress.
Specific embodiment
Embodiment 1
Living body embryonated egg 0kg adds 50kg soybean protein isolate.First the kind after black line hair removes no fertilized eggs is blocked with candle
Egg 10kg, each hatching egg is based on 45 grams, and about 222.Hatch in 37.8 degree of artificial incubation device, is tested again with candler within the 5th day
Egg is primary, chooses no fertilized eggs.
Between during hatching 17 to 19 days, living body is removed to after the disinfection treatment of egg shell within this example selected 18 days, gone
After falling stillborn foetus, adding water and soybean protein isolate mixing to size mixing after low-temperature grinding processing makes it containing solid content between 12% to 15%.
With a small amount of disinfectant mixing compound protease, i.e. allotment liquid of protease, compound enzyme dosage is 0.3% -0.5%.This
75 grams of compound proteases are added by 0.3% allotment in secondary enzyme dosage.Then the protease of the protein raw materials and allotment that living body are crushed
Liquid mixing heating, temperature control which achieves enzymatic hydrolysis while mixing slowly, enzymolysis time arrives for 68 between 54 degree to 56 degree
Between 72 hours, this example 68 hours of selected enzymatic hydrolysis, entire enzymolysis process is not required to adjust pH value
Hatching egg after this example 222 screenings removes 2 again after hatching five days and there is not fertile egg, and disinfection shelling also removes 1
Stillborn foetus, it is real in total to obtain 219 living body embryos, remove eggshell 1.5kg and 0.5kg non-living body egg, obtain embryo 8.5kg embryo living body egg
White liquor.By 70% is aqueous plus water based on this example obtain 2.6kg, and 25kg protein isolate altogether in 27.6kg material protein addition 206.6kg
Water mixes embryo's albumen containing solid 12% feed liquid, through 68 hours enzymolysis processings.
Processing is centrifuged in feed liquid after enzymatic hydrolysis, large particulate matter is removed out, obtains fully dissolved and exempt from digesting protein
Liquid form product.
Exempt from digestible protein liquid to fully dissolved to be concentrated through double effect evaporator, is concentrated to 40% or more solid content, is spray-dried
Obtain powdery exempts from digesting protein product.It obtains 26kg and exempts from digesting protein powder, add 20kg cocoa liquor, add 16kg cocoa butter,
Add 15kg granulated sugar.
With steamer discharge water heating then equipped with exempt from digestible protein powder, cocoa liquor, cocoa butter, white granulated sugar container be put into
On steamer all waters surface.Side heating is kept stirring, until container object in the pasty state, can be helped adjust sticky with a small amount of milk, reached
Spoon can descend drop, but not quickly, pour into mold, natural cooling, demoulding packaging after mixing evenly.
Example 2
Living body embryonated egg 10kg adds 20kg soybean protein isolate.First the kind after black line hair removes no fertilized eggs is blocked with candle
Egg 10kg, each hatching egg is based on 45 grams, and about 222.Hatch in 37.8 degree of artificial incubation device, is tested again with candler within the 5th day
Egg is primary, chooses no fertilized eggs.
Between during hatching 17 to 19 days, living body is removed to after the disinfection treatment of egg shell within this example selected 18 days, gone
After falling stillborn foetus, adding water and soybean protein isolate mixing to size mixing after low-temperature grinding processing makes it containing solid content between 12% to 15%.
With a small amount of disinfectant mixing compound protease, i.e. allotment liquid of protease, compound enzyme dosage is 0.3% -0.5%.This
75 grams of compound proteases are added by 0.3% allotment in secondary enzyme dosage.Then the protease of the protein raw materials and allotment that living body are crushed
Liquid mixing heating, temperature control which achieves enzymatic hydrolysis while mixing slowly, enzymolysis time arrives for 68 between 54 DEG C to 56 DEG C
Between 72 hours, this example 68 hours of selected enzymatic hydrolysis, entire enzymolysis process is not required to adjust pH value.
Hatching egg after this example 222 screenings removes 2 again after hatching five days and there is not fertile egg, and disinfection shelling also removes
1 stillborn foetus, real 219 living body embryos in total, remove eggshell 1.5kg and 0.5kg non-living body egg, embryo 8.5kg embryo
Living body protein liquid.By 70% is aqueous plus water based on this example obtain 2.6kg, and 20kg protein isolate altogether in the addition of 22.6kg material protein
117.5kg water mixes embryo's albumen containing solid 12% feed liquid, through 68 hours enzymolysis processings.
Processing is centrifuged in feed liquid after enzymatic hydrolysis, large particulate matter is removed out, obtains fully dissolved and exempt from digesting protein
Liquid form product.
Exempt from digestible protein liquid to fully dissolved to be concentrated through double effect evaporator, is concentrated to 40% or more solid content, is spray-dried
Obtain powdery exempts from digesting protein product.It obtains 21.5kg and exempts from digesting protein powder, add 25kg cocoa liquor, add 16kg cocoa
Rouge adds 10kg granulated sugar.
With steamer discharge water heating then equipped with exempt from digestible protein powder, cocoa liquor, cocoa butter, white granulated sugar container be put into
On steamer all waters surface.Side heating is kept stirring, until container object in the pasty state, can be helped adjust sticky with a small amount of milk, reached
Spoon can descend drop, but not quickly, pour into mold, natural cooling, demoulding packaging after mixing evenly.
Claims (4)
1. a kind of instant active double protein is the method that raw material prepares that solid-state exempts from digesting protein, it is characterised in that: double protein is
The solid-state of raw material exempts from digestible protein class chocolate；Function is freezed off by the hydrothermal solution of cocoa liq-uor rouge, product is become instant
Exempt from digestible protein class chocolate；The fragrance of cocoa liq-uor and cocoa butter has been completely covered by the offending taste of peptide, ideal;
Manufacturing process steps are:
1., by taking fertilized eggs as an example, fertilization hatching egg is through artificial incubation (use incubator) Constant temperature hatch method, incubation temperature are as follows:
Hatch at a temperature of 37.8, fertile egg is made to be changed into chicken son's tire albumen mixing soya beans albumen living as double protein raw material, containing height
Active high nutrition ingredient；It is primary with candler candling through hatching the 5th day, remove no fertilized eggs；
2., pearl living will be hatched in 1 to except before young, using embryo's living body biological, to egg shell between hatching 17 to 19 days
Living body is removed after disinfection treatment, adding water to size mixing after low-temperature grinding processing makes it containing solid content between 12% to 15%；
3., with a small amount of disinfectant mixing compound protease, i.e., allotment liquid of protease, compound enzyme dosage are 0.3% -0.5%；
4., step 2 is mixed with step 3 with soybean protein raw material after (animal protein and vegetable protein ratio are 1 to 10 or so)
Heating, temperature control which achieves enzymatic hydrolysis while mixing slowly between 54 DEG C to 56 DEG C, and enzymolysis time is 68 hours to 72
Between hour, entire enzymolysis process is not required to adjust pH value；
5., by step 4 enzymatic hydrolysis after the completion of feed liquid carry out the isolated lysate for exempting from digestible protein of seperator；
6., step 5 is carried out to sterilization and destroy the enzyme treatment, feed liquid reaches 75 DEG C -90 DEG C in temperature through coil sterilizer, heat preservation 15
- 16 seconds second, reach sterilising and enzyme inactivating purpose；
7., by step 6 by double effect evaporator concentration material to solid content 40% or more, be spray-dried to obtain and exempt to digest
Small-molecular peptides (containing compound amino acid) protein powder product.
2. the preparation method of digesting protein is exempted from a kind of activated protein original preparation according to claim 1, feature exists
In: activated protein is former.
3. using kind of a fertilized eggs egg to be hatched live embryo as albumen and soybean protein is raw material, embryo viability substance is saved；
Enzymatic hydrolysis obtains tool placenta is active exempts from digesting protein product for a long time；The product by dipeptides tri-peptide molecule amount 300DA to
The compound amino acid of 500DA and average molecular weight in 125DA forms.
4. uncomfortable ph value freely digests in technique, the temperature for carrying out sterilization and enzyme deactivation controls the heat preservation 15-16 at 75 DEG C-90 DEG C
Second, biological embryo viability is not destroyed.
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| CN111616371A (en) * | 2019-12-26 | 2020-09-04 | 长春大学 | Preparation method for producing digestion-free protein by using various egg white proteins as raw materials |
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Effective date of registration: 20190723 Address after: 154011 Sihe Village, Changqing Township, Jiamusi Suburb, Heilongjiang Province (Jiamusi Economic and Technological Development Zone, West Friendship Road) Applicant after: JIAMUSI DONGMEI SOYBEAN FOOD Co.,Ltd. Address before: 130022 Changchun City, Jilin province Satellite Road, No. 6543 Applicant before: Changchun University |
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