CN118084573A - Aquatic product compound fertilizer based on fermented cow dung and use method and application thereof - Google Patents

Aquatic product compound fertilizer based on fermented cow dung and use method and application thereof Download PDF

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Publication number
CN118084573A
CN118084573A CN202410480298.4A CN202410480298A CN118084573A CN 118084573 A CN118084573 A CN 118084573A CN 202410480298 A CN202410480298 A CN 202410480298A CN 118084573 A CN118084573 A CN 118084573A
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cow dung
compound fertilizer
fermented cow
fertilizer
aquatic
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董晓庆
王桂芹
曲桂娟
牛小天
陈秀梅
姜丹
吴雪芹
李畅
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Jilin Agricultural University
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Jilin Agricultural University
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Abstract

The invention provides an aquatic compound fertilizer based on fermented cow dung, and a use method and application thereof, belonging to the technical field of aquatic compound fertilizers; the aquatic compound fertilizer comprises the following raw materials in parts by weight: fermented cow dung, inorganic fertilizer and biological water modifier = (75-78): (2.8-6.1): (18.9-19.2). The invention adopts cow dung ferment, inorganic fertilizer and water quality modifier to prepare the special fertilizer for fish through scientific compound proportioning, has the advantages of low consumption, quick effect, lasting fertilizer efficiency and no pollution to the cultivation ecology, forms cultivation water with rich algae groups and light green and fresh water color, and increases natural bait organisms.

Description

Aquatic product compound fertilizer based on fermented cow dung and use method and application thereof
Technical Field
The invention belongs to the technical field of aquatic compound fertilizers, and particularly relates to an aquatic compound fertilizer based on fermented cow dung, and a use method and application thereof.
Background
Filter feeding fishes feed on plankton (algae, cladocera, copepods, rotifers and the like), and are key in water quality fertilization. At present, the fertilizer application of the culture water body mainly comprises organic fertilizer, inorganic fertilizer and compound fertilizer. The organic fertilizer has the advantages of comprehensive nutrition as a traditional fertilizer, but has slower fertilizer efficiency, longer utilization process of plankton, consumption of a large amount of oxygen in water in the decomposition and mineralization process, easy water pollution, adverse effect on the growth of fish, limitation of seasons in use of the organic fertilizer, and use of the organic fertilizer as a base fertilizer mainly in seasons with lower water temperature in winter and spring. Compared with organic fertilizers, inorganic fertilizers are quick in fertilizer application, but have single functions, a large amount of application can pollute water quality, harmful bacteria cannot be controlled, fish diseases cannot be effectively prevented, and therefore the water quality of aquatic products is affected.
Therefore, there is a need to provide an aquatic compound fertilizer special for cultivating freshwater fish and a preparation method thereof, wherein the aquatic compound fertilizer has the advantages of low consumption, quick response, lasting fertilizer efficiency and no pollution to cultivation ecology.
Disclosure of Invention
In order to solve the technical problems, the invention provides an aquatic compound fertilizer based on fermented cow dung, and a use method and application thereof.
In order to achieve the above purpose, the present invention provides the following technical solutions:
One of the technical schemes of the invention is as follows:
an aquatic compound fertilizer based on fermented cow dung comprises the following raw materials in parts by weight:
Fermented cow dung, inorganic fertilizer and biological water modifier = (75-78): (2.8-6.1): (18.9-19.2).
Preferably, the aquatic compound fertilizer based on the fermented cow dung comprises the following raw materials in percentage by weight:
fermented cow dung, inorganic fertilizer and biological water quality modifier=75:6.1:18.9.
Preferably, the aquatic compound fertilizer based on the fermented cow dung comprises the following raw materials in percentage by weight:
Fermented cow dung, inorganic fertilizer and biological water quality improver=78:2.8:19.2.
Preferably, the preparation method of the fermented cow dung comprises the following steps: and composting and fermenting cow dung, rice bran, corn flour, brown sugar and microbial agent. Wherein the cow dung is fresh cow dung with the water content of 70%; the rice bran as an organic matter can provide energy required by growth and reproduction for the organic matter, promote the growth and reproduction of microorganisms, reduce the generation of ammonia gas, and is one of the sources of carbon sources in the aquatic compound fertilizer; the effects of adjusting moisture, ventilation and carbon-nitrogen ratio can be achieved; the brown sugar provides energy sources in the fermentation process, promotes the propagation and activity of microorganisms, accelerates the decomposition process of organic matters, is one of the sources of carbon sources in the aquatic compound fertilizer, and can also promote the fermentation.
Further, the mass ratio of the cow dung to the rice bran to the corn flour is 14:3:3;
The addition amount of the brown sugar and the microbial agent is 0.2wt.% and 0.3wt.% respectively (wherein the mass percentage is the mass percentage of the sum of the cow dung, the rice bran and the corn meal).
The beneficial effects are that: the existing carbon source for aquaculture has no support of 'carbon fertilizer', the bacterial effect is short and is not sustainable, the 'bacterial-algae balance' is difficult to maintain, and the 'fermentation carbon source' is an important carbon source in the carbon source, and the brown sugar defined by the invention is a quick-acting carbon source, can promote fermentation and has good effect.
Further, the microbial agent consists of cellulolytic bacteria and bacillus subtilis according to the mass ratio of 3:1. Wherein the cellulolytic bacteria is Aureobasidium pullulans (Aureobasidium pullulans); the bacillus subtilis is bacillus subtilis (Bacillus subtilis).
Furthermore, the effective viable bacteria number of the cellulolytic bacteria and the bacillus subtilis is more than or equal to 10 8/mL.
Further, the composting fermentation conditions are as follows: the water content (mixture) is kept at 60 percent, and the fermentation is carried out for 5 to 10 days under the sealing condition at the temperature higher than 22 ℃.
Preferably, the inorganic fertilizer is nitrogen, phosphorus and potassium fertilizer, wherein the mass ratio of nitrogen, phosphorus and potassium is 1:1:0.5.
Further, the inorganic fertilizer is a plurality of urea, diammonium phosphate, monopotassium phosphate and calcium superphosphate.
Preferably, the biological water quality improver is one or more of enterococcus faecalis, bacillus licheniformis or saccharomycetes.
Further, the biological water quality improver is enterococcus faecalis (Enterococcus faecalis), bacillus licheniformis (Bacillus licheniformis) and saccharomyces cerevisiae (Saccharomyces cerevisiae).
The beneficial effects are that: the invention takes lactic acid enterococcus faecalis as a main material and is matched with bacillus licheniformis and saccharomycetes. The lactobacillus phase of the water body can be periodically supplemented, and the assimilation ammonia nitrogen and nitrate can be absorbed; forming a symbiotic coexisting ecological water body of a lactobacillus phase and a diatom phase; and can decompose organic scraps, tender and cool water quality, promote the formation and stability of beneficial algae phases.
The second technical scheme of the invention is as follows:
the application method of the aquatic compound fertilizer based on the fermented cow dung comprises the following steps:
(1) Weighing the raw material dosage of the aquatic compound fertilizer based on the fermented cow dung for standby;
(2) Adding biological water quality modifier into fermented cow dung, mixing, and sprinkling to pond; then applying inorganic fertilizer.
The beneficial effects are that: the biological water quality modifier is used for adjusting water quality after adding organic fertilizer, belongs to biological modifier, and is obtained by calculating according to pond water quantity; the water quality improver is added after being mixed with the organic fertilizer to ensure that the water quality improver can be uniformly dispersed in the water body; the inorganic fertilizer and the organic fertilizer are added separately because the inorganic fertilizer needs to be fully dissolved and then used, so that the fertilizer efficiency is quickly started in the water body, and the independent addition is used for avoiding uneven mixing of solid (organic fertilizer) liquid (inorganic fertilizer) and is more convenient to operate than the addition after mixing.
Preferably, the inorganic fertilizer application sequence is as follows: firstly, applying calcium superphosphate and then applying nitrogen, phosphorus and potassium fertilizers; wherein, the nitrogen, phosphorus and potassium fertilizers are directly mixed evenly or are sprayed after being dissolved. Wherein, because the superphosphate belongs to acid chemical phosphate fertilizer, and contains 3.5% -5% of phosphorus, calcium and phosphorus can be decomposed in water, which is beneficial to the growth of algae. The calcium superphosphate is firstly applied, and then the nitrogen, phosphorus and potassium fertilizers are applied because the calcium superphosphate and the nitrogenous fertilizers such as urea are mixed to generate chemical reaction to form ammonium phosphate salts, so that the fertilizer efficiency is reduced, and the calcium superphosphate, the nitrogen, the phosphorus and the potassium fertilizers are independently used.
The third technical scheme of the invention:
the invention also discloses application of the aquatic compound fertilizer based on the fermented cow dung in the field of freshwater aquaculture.
Compared with the prior art, the invention has the following advantages and technical effects:
The invention is mainly used for aquaculture, is developed aiming at the problem of carbon deficiency in aquaculture water, and solves the problem of carbon deficiency in aquaculture water by adding rice bran, corn flour and brown sugar into fermented cow dung as main sources of carbon sources. Carbon is an important nutrient element of algae, carbon dioxide is needed during photosynthesis of the algae, and as the photosynthesis of the algae continuously consumes bicarbonate ions in water, the carbon source is less and less, the carbon source is insufficient, the algae can not absorb and utilize the carbon source, ammonia nitrogen is increased, and finally nitrite is increased. The lack of carbon source and the lack of energy of microorganisms in the water body can not effectively decompose organic matters in the water body, and can cause water quality deterioration. Therefore, the invention increases the carbon source in the culture water body, thereby accelerating the decomposition of nitrogen element and improving the ecological environment of the culture water. The carbon-nitrogen ratio of the water body is regulated by increasing the carbon source, so that the virtuous circle of the culture water body is promoted. The addition of the nitrogen, phosphorus and potassium fertilizer can quickly fertilize water, is beneficial to algae propagation, and can reflect the growth condition of algae.
In conclusion, the special fertilizer for fish is developed by adopting cow dung ferment, inorganic fertilizer and water quality modifier in proportion and scientific compound proportion, has the advantages of low consumption, quick response, lasting fertilizer efficiency and no pollution to the cultivation ecology, forms a cultivation water body with rich algae groups and light green and fresh water color, and increases natural bait organisms.
Drawings
The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this specification, illustrate embodiments of the application and together with the description serve to explain the application. In the drawings:
FIG. 1 is a bar graph of phytoplankton levels before and after application of the aquatic compound fertilizer prepared in example 1 to pond 1;
FIG. 2 is a bar graph of phytoplankton levels before and after pond 2 is subjected to the aquatic compound fertilizer prepared in example 2;
FIG. 3 is a graph showing statistics of various algae occupancy rates after pond 1 is applied with the aquatic compound fertilizer prepared in example 1;
FIG. 4 is a graph showing statistics of various algae occupancy rates after pond 2 is applied with the aquatic compound fertilizer prepared in example 2;
FIG. 5 is a bar graph of zooplankton amounts before and after pond 1 is applied with the aquatic compound fertilizer prepared in example 1;
FIG. 6 is a bar graph of zooplankton amounts before and after pond 2 is applied with the aquatic compound fertilizer prepared in example 2;
FIG. 7 is a graph showing the statistics of the ratio of zooplankton to the water-based compound fertilizer prepared in example 1 applied to pond 1;
FIG. 8 is a graph showing the statistics of the ratio of zooplankton to the water-based compound fertilizer prepared in example 2 applied to pond 2;
FIG. 9 is a graph of the amount of phytoplankton in four pond bodies of water to which the fertilizer of examples 1-2 and comparative examples 1-2 were applied;
FIG. 10 is a graph of the zooplankton content of four pond bodies of water to which the fertilizers of examples 1-2 and comparative examples 1-2 were applied.
Detailed Description
Various exemplary embodiments of the invention will now be described in detail, which should not be considered as limiting the invention, but rather as more detailed descriptions of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. In addition, for numerical ranges in this disclosure, it is understood that each intermediate value between the upper and lower limits of the ranges is also specifically disclosed. Every smaller range between any stated value or stated range, and any other stated value or intermediate value within the stated range, is also encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the invention described herein without departing from the scope or spirit of the invention. Other embodiments will be apparent to those skilled in the art from consideration of the specification of the present invention. The specification and examples of the present invention are exemplary only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are intended to be inclusive and mean an inclusion, but not limited to.
The raw materials used in the examples of the present invention are all commercially available. The invention relates to a preservation unit of microorganisms, which is China center for type culture Collection of industrial microorganisms, wherein enterococcus faecalis (Enterococcus faecalis) is preserved: CICC 23211; bacillus subtilis (Bacillus subtilis), accession number: CICC 24713; cellulolytic bacteria (aureobasidium pullulans Aureobasidium pullulans), deposit number: CICC 2696; bacillus licheniformis (Bacillus licheniformis), accession number CICC 10084; yeast (Saccharomyces cerevisiae Saccharomyces cerevisiae), accession number CICC 31965.
The technical scheme of the invention is further described by the following examples.
Example 1
The preparation process of the fermented cow dung is as follows:
(1) Weighing the raw material components according to the following dosage for standby; the raw materials of the fermented cow dung comprise cow dung, rice bran and corn flour, and the mass ratio of the cow dung to the rice bran to the corn flour is 14:3:3. In addition, the microbial agent also comprises brown sugar (the addition amount of the brown sugar is 0.2wt.% of the total weight of the three raw materials) and a compound microbial agent (the microbial agent consists of cellulolytic bacteria and bacillus subtilis, wherein the effective viable count of the cellulolytic bacteria and the bacillus subtilis is more than or equal to 10 8/mL, the mass ratio of the cellulolytic bacteria to the bacillus subtilis is 3:1, and the addition amount of the compound microbial agent is 0.3wt.% of the total weight of the three raw materials).
(2) According to the dosage, the composite microbial agent is firstly mixed with rice bran and corn meal, then evenly mixed with cow dung, and then added with brown sugar for even stirring (wherein, the addition of a proper amount of brown sugar can promote fermentation), and composting fermentation is carried out; the fermentation conditions are as follows: adjusting water content to 60%, sealing and fermenting at 25deg.C for 5 days to obtain fermented cow dung with yeast flavor and acid flavor.
The fermented cow dung is detected by referring to a NY 525-2012 organic fertilizer detection method, and the detection results are shown in Table 1.
TABLE 1 detection results of related indicators of fermented cow dung
Conclusion: the results in Table 1 show that the produced fermented cow dung completely meets the NY 525-2012 organic fertilizer standard.
(II) aquatic compound fertilizer based on fermented cow dung and application method thereof
The aquatic compound fertilizer based on the fermented cow dung comprises the following raw material components in percentage by mass:
The mass ratio of the fermented cow dung to the inorganic fertilizer to the water quality improver is 75:6.1:18.9;
wherein the mass ratio of nitrogen, phosphorus and potassium in the inorganic fertilizer is 1:1:0.5;
The biological water quality modifier consists of lactic acid bacteria enterococcus faecalis, bacillus licheniformis and saccharomycetes, wherein the mass ratio of the lactic acid bacteria enterococcus faecalis to the bacillus licheniformis to the saccharomycetes is 1:2:1.
The application method of the aquatic compound fertilizer based on the fermented cow dung comprises the following steps: the method comprises the following specific steps of:
(1) Adding biological water quality modifier into fermented cow dung, mixing thoroughly, and sprinkling in a whole pond;
(2) The inorganic fertilizer is prepared by firstly applying calcium superphosphate (the phosphorus in the calcium superphosphate accounts for 7.64wt.% of the total phosphorus), and then applying nitrogen, phosphorus and potassium fertilizer; wherein, the nitrogen, phosphorus and potassium fertilizers are directly and evenly mixed and then are sprinkled.
Example 2
Compared with the example 1, the mass ratio of the fermented cow dung, the inorganic fertilizer and the water quality modifier in the aquatic compound fertilizer is 78:2.8:19.2. Other conditions were the same as in example 1.
Example 3
In comparison with example 1, the fermentation time in step (2) was 7 days during the preparation of fermented cow dung. Other conditions were the same as in example 1.
Example 4
In comparison with example 1, the fermentation time in step (2) was 10 days during the preparation of fermented cow dung. Other conditions were the same as in example 1.
Comparative example 1
The difference from example 1 is that only an organic fertilizer (organic cow dung) was applied, and the other conditions were the same as in example 1.
Comparative example 2
The difference from example 1 is that only the inorganic fertilizer was applied, and the other conditions were the same as in example 1.
Compared with the condition of singly using inorganic fertilizer and organic fertilizer, the aquatic compound fertilizer has better fertilizer-water effect.
Effect verification
1. Amount of phytoplankton after fertilization in pond
According to the aquatic compound fertilizer based on fermented cow dung in the embodiment 1 and the application method thereof, the ponds 1 and 2 (the water capacity of the two ponds is 2 multiplied by 10 3 L) are respectively fertilized, after the fertilization, the phytoplankton in the ponds 1 and 2 begin to appear in peak period from 8 days to 10 days, the phytoplankton types mainly comprise blue algae, green algae and diatom, and a small amount of cryptophycin, dinoflagellate and euglena also appear, and the method is particularly shown in the figure 1 and the figure 2.
Figures 3 and 4 show statistical graphs of average ratios of various algae in pond 1 and pond 2, respectively, after fertilization, wherein green algae account for 61.89%, blue algae account for 17.28%, diatom account for 20.53%, dinoflagellate 0.06% and cryptophycin 0.24% in phytoplankton in pond 1;
in the pond 2, phytoplankton green algae account for 80.254%, blue algae account for 9.121%, diatom accounts for 9.734%, dinoflagellate 0.817% and cryptophycin 0.074%.
2. Zooplankton amount after pond fertilization
As shown in fig. 5, the zooplankton in pond 1 starts to peak at day 8, with zooplankton species being predominantly rotifers, copepods, cladocera and nauplii. Wherein, the ratio of rotifer is 54.41%, the ratio of copepods is 40.07%, the ratio of cladocera is 0.37%, and the ratio of nauplii is 5.15%, and the detailed description is shown in figure 7.
As shown in fig. 6, pond 2 zooplankton started to peak at day 10, with zooplankton species being predominantly rotifers, copepods, cladocera and nauplii. Wherein, the ratio of rotifer is 61.41%, the ratio of copepods is 26.56%, the ratio of cladocera is 1.24%, and the ratio of nauplii is 10.79%, and the detailed description is shown in figure 8.
3. According to examples 1 and 2 and comparative examples 1 and 2 (examples 1 and 2, comparative examples 1 and 2 correspond to pond 1, pond 2, pond 3, pond 4, respectively), it can be seen from fig. 9 and 10 that the phytoplankton has started to reproduce from the next day and has doubled on day 6, the phytoplankton has reached the peak on day 8, and the phytoplankton is the best biological feed for the zooplankton, and the zooplankton amounts in both ponds have reached the peak on days 8 and 10, respectively, as can be seen from the change in pond water color and the measurement of the phytoplankton in 3-5 days after fertilization, which indicates that the zooplankton has rapidly proliferated with the nutrients of the fertilizer after fertilization, and that the fertilizer efficiency is rapid. Meanwhile, the water quality of the pond is monitored, and the water quality parameters of the pond after fertilization completely meet the requirements of the freshwater fish-farming water body. Namely, the aquatic compound fertilizer achieves a better fertilizer-water effect than the case of using the inorganic fertilizer and the organic fertilizer alone (wherein, in fig. 9 and 10, the compound fertilizer # 1 is the compound fertilizer of example 1, the compound fertilizer # 2 is the compound fertilizer of example 2, the organic fertilizer # 3 is the inorganic fertilizer # 1, and the inorganic fertilizer # 4 is the compound fertilizer # 2). The parameters of the culture water bodies in the fresh water pond 1 and the fresh water pond 2 are shown in table 2.
Table 2 parameters of the bodies of aquaculture water in freshwater ponds 1 and 2
Conclusion: the results in table 2 show that pond water quality after fertilization according to examples 1 and 2 meets the requirements of freshwater aquaculture.
In conclusion, the aquatic compound fertilizer defined by the invention has the advantages of low consumption, quick response, lasting fertilizer efficiency and no pollution to the culture ecology, forms a culture water body with rich algae groups and light green and fresh water color, and increases natural bait organisms.
The present application is not limited to the above-mentioned embodiments, and any changes or substitutions that can be easily understood by those skilled in the art within the technical scope of the present application are intended to be included in the scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims (10)

1. An aquatic compound fertilizer based on fermented cow dung is characterized in that the raw materials comprise the fermented cow dung, an inorganic fertilizer and a biological water quality modifier; wherein, according to the weight ratio:
Fermented cow dung, inorganic fertilizer and biological water modifier = (75-78): (2.8-6.1): (18.9-19.2).
2. The aquatic compound fertilizer based on fermented cow dung according to claim 1, wherein the aquatic compound fertilizer is characterized by comprising the following components in percentage by weight:
fermented cow dung, inorganic fertilizer and biological water quality modifier=75:6.1:18.9.
3. The aquatic compound fertilizer based on fermented cow dung according to claim 1, wherein the aquatic compound fertilizer is characterized by comprising the following components in percentage by weight:
Fermented cow dung, inorganic fertilizer and biological water quality improver=78:2.8:19.2.
4. The aquatic compound fertilizer based on fermented cow dung according to claim 1, wherein the preparation method of the fermented cow dung is as follows: and composting and fermenting cow dung, rice bran, corn flour, brown sugar and microbial agent.
5. The aquatic compound fertilizer based on fermented cow dung according to claim 4, wherein the mass ratio of cow dung to rice bran to corn flour is 14:3:3;
the addition amounts of the brown sugar and the microbial agent are 0.2wt.% and 0.3wt.%, respectively.
6. The aquatic compound fertilizer based on fermented cow dung of claim 4, wherein the microbial agent consists of cellulolytic bacteria and bacillus subtilis according to a mass ratio of 3:1.
7. The aquatic compound fertilizer based on fermented cow dung according to claim 4, wherein the composting fermentation conditions are as follows: keeping water content at 60%, and sealing and fermenting at a temperature higher than 22deg.C for 5-10 days.
8. The aquatic compound fertilizer based on fermented cow dung according to claim 1, wherein the biological water quality modifier is one or more of enterococcus faecalis, bacillus licheniformis or saccharomycetes.
9. The application method of the aquatic compound fertilizer based on the fermented cow dung is characterized by comprising the following steps of:
(1) Weighing the raw material amount of the aquatic compound fertilizer based on fermented cow dung according to any one of claims 1-8 for later use;
(2) Adding biological water quality modifier into fermented cow dung, mixing, and sprinkling to pond; and then inorganic fertilizer is sprayed.
10. Use of an aquatic compound fertilizer based on fermented cow dung according to any one of claims 1-8 in the field of freshwater aquaculture.
CN202410480298.4A 2024-04-22 2024-04-22 Aquatic product compound fertilizer based on fermented cow dung and use method and application thereof Pending CN118084573A (en)

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CN104206716A (en) * 2014-08-27 2014-12-17 武汉瑞泽园生物环保科技有限公司 Biological organic aquaculture nutrition fertilizer powder and preparation method thereof
CN110973027A (en) * 2019-12-31 2020-04-10 天津开发区坤禾生物技术有限公司 Method for ecologically breeding litopenaeus vannamei by replacing part of feed with live bait

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