CN117837565B - Raising method for improving appearance quality of hepialus larva - Google Patents

Raising method for improving appearance quality of hepialus larva Download PDF

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CN117837565B
CN117837565B CN202410263017.XA CN202410263017A CN117837565B CN 117837565 B CN117837565 B CN 117837565B CN 202410263017 A CN202410263017 A CN 202410263017A CN 117837565 B CN117837565 B CN 117837565B
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hepialus
feeding
larva
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raising
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CN117837565A (en
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牟雪
张翰熙
余欢
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Sichuan Zhenyuan Biotechnology Co ltd
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Abstract

The invention belongs to the technical field of indoor artificial cultivation of cordyceps sinensis, and particularly relates to a raising method for improving appearance quality of hepialus armoricanus larvae. The invention provides a raising method for improving the appearance quality of hepialus larvae, which comprises the following steps: raising hepialus larva at the ambient temperature of 5-16 ℃ and the humidity of 60-90% under the dark condition, selecting 5-8-year-old hepialus larva to be put into a raising box for raising, and placing one hepialus larva in each raising box; the fodder is placed in the feeding box, and the fodder is radix Potentillae Anserinae, herba Polygoni Avicularis and radix Dauci Sativae, or radix Potentillae Anserinae, herba Polygoni Avicularis and rhizoma Fagopyri Dibotryis tuber; the feeding substrate is not placed in the feeding box. After the hepialus larva fed by the method disclosed by the invention is formed into the finished cordyceps sinensis, the appearance of the spots of the worm bodies can be effectively controlled, and the overall appearance quality of the finished cordyceps sinensis is improved.

Description

Raising method for improving appearance quality of hepialus larva
Technical Field
The invention belongs to the technical field of indoor artificial cultivation of cordyceps sinensis, and particularly relates to a raising method for improving appearance quality of hepialus armoricanus larvae.
Background
The Cordyceps sinensis host hepialus larva mainly living in a plateau soil layer in a natural environment, the activity depth is about 20 cm-100 cm, single or mixed culture matrixes such as plateau soil, peat soil and coconut husk are mainly adopted for artificially culturing the hepialus larva at present, and the finished product Cordyceps sinensis can be obtained through culturing, however, spots and uneven colors appear on the body of the produced finished product Cordyceps sinensis.
Disclosure of Invention
The invention provides a breeding method of high-age hepialus larva, which can effectively control the appearance of spots on worm bodies after forming finished cordyceps sinensis products, and improve the overall appearance quality of the finished cordyceps sinensis products.
According to a specific embodiment of the invention, a raising method for improving the appearance quality of hepialus larvae, namely a method for raising high-age hepialus larvae, comprises the following steps:
Selecting 5-8-year-old hepialus larvae to be fed in feeding boxes, wherein one hepialus larva is arranged in each feeding box;
Placing a feed in the feeding box, wherein the feed comprises one or more of radix potentillae anserinae tubers, polygonum aviculare tubers, carrots and wild buckwheat tubers;
The feeding substrate is not placed in the feeding box.
In the invention, the feeding substrate is a substance providing an environmental basis for the growth of hepialus larvae, the wild hepialus larvae live in plateau soil, the plateau soil is a growth substrate of the wild hepialus larvae, the existing artificial feeding technology simulates the growth substrate of the wild hepialus larvae, for example, the feeding substrate mainly selected by the artificial feeding hepialus larvae comprises organic soil without pesticide residues, coco coir, or soil/coco coir mixed substrate prepared in different proportions, and the like.
According to the invention, the clanis hepialid larva is bred in a soilless (feeder matrix-free) mode, the control difficulty of the soilless breeding environment is high, the environmental stability is lower than that of the soil breeding, in order to ensure the survival rate of the clanis hepialid larva in the soilless environment, the clanis hepialid larva with 5-8 ages is selected, and the clanis hepialid larva is bred in a breeding box for 40-60 days, so that the hardening or pupation of the clanis hepialid larva can be completed, the survival rate of the clanis hepialid larva is relatively high, and the occurrence of spots on an insect body can be effectively controlled.
In the invention, the hepialus larva in the 5-8-year-old hepialus larva refers to the hepialus larva which is already infected with hirsutella sinensis or the hepialus larva which is not already infected with hirsutella sinensis.
According to the breeding method for improving the appearance quality of hepialus larvae, according to the specific embodiment of the invention, the hepialus larvae are bred under the condition that the environment temperature is 5-16 ℃ and the humidity is 60-90% and no light exists.
According to the feeding method for improving the appearance quality of hepialus larvae in the specific embodiment of the invention, the feed placed in the feed box is replaced every 8-12 days.
According to the feeding method for improving the appearance quality of hepialus larvae, according to the specific embodiment of the invention, the feed is silverweed cinquefoil root tuber, polygonum perfoliatum root tuber and carrot (root tuber), or the feed is silverweed cinquefoil root tuber, polygonum perfoliatum root tuber and wild buckwheat tuber.
According to the feeding method for improving the appearance quality of hepialus larvae, which is provided by the specific embodiment of the invention, the weight ratio of the silverweed cinquefoil root tuber to the polygonum orientale root tuber to the carrot is 1-2:1-3:1-3; the weight ratio of the radix potentillae anserinae tubers, the polygonum capitatum tubers and the wild buckwheat tubers is 1-2:1-3:1-3.
According to the feeding method for improving the appearance quality of hepialus larvae, which is an embodiment of the invention, the total weight of feed placed in a feeding box is 20-30g.
According to the breeding method for improving the appearance quality of hepialus larvae, which is an embodiment of the invention, the breeding time of the hepialus larvae is 40-60 days.
According to the breeding method for improving the appearance quality of the hepialus larva, according to the specific embodiment of the invention, after the hepialus larva is cultivated for 30 days, stiff worms and dead worms are screened out, then the stiff worms and the dead worms are screened every 10 days, the hepialus larva is cultivated for 60 days, and the cultivation is finished after the 4 th screening is finished.
In order to avoid the contamination of mixed bacteria, the feeding boxes and feeds used in the invention are strictly sterilized and used after the bacteria inspection is qualified;
Preferably, the breeding environment of hepialus larvae is a clean room, which is also called a clean room, and is a special environment control, in which the number of particles in the air, humidity, temperature, static electricity and other factors can be controlled to achieve a specific cleaning standard. Clean rooms are divided into four classes, respectively: A. b, C, D. Preferably, the breeding environment for hepialus larvae is a class C clean room, also known as a ten-thousand clean room, or class C clean room, which has a level of cleanliness that allows for up to 352000 (static) 352,0000 particles per cubic meter of air of 0.5um or more.
The culture box used in the utility model is a single-bin culture box, the structure of the culture box comprises a box body and a box cover, the box body is divided into a plurality of feeding bins for feeding one larva independently through a partition, one side of the box cover is provided with a caulking groove matched with the partition, the upper part of the partition can be embedded into the caulking groove, the box cover is covered on the box body through the matching of the caulking groove and the partition and seals the feeding bins, the positions of the box cover corresponding to the feeding bins are all provided with ventilation holes, the ventilation holes are covered with ventilation films, and the structure of the single-bin culture box can be seen in the patent of the utility model with the application number 202322730792.5.
In order to further improve the effect of the single-bin culture box, the single-bin culture box is improved to be of a net structure on the basis of the single-bin culture box, the aperture is 2mm x 2mm and is used for leaking and dropping the excrement and the feed residues of the larvae, meanwhile, the bottom is additionally provided with the material receiving tray for containing the excrement and the feed residues of the hepialus larvae, and timely cleaning can be conveniently carried out.
The invention has the beneficial effects that:
Firstly, under the condition of soil feeding, the invention finds that the excrement and rotten feed of the hepialus larva can influence the small environment for the growth of the hepialus larva, the humidity in the feeding matrix is uneven or mixed bacteria are generated in the feeding matrix, so as to infect the surface of the stiff worm, and finally, the situation that spots and uneven colors appear on the surface of the semi-finished product stiff worm is caused, and the appearance quality is seriously influenced.
Secondly, the invention adopts a mode of soilless (without a feeding matrix) and single-bin feeding of hepialus larvae, correspondingly determines the age of the hepialus larvae capable of being fed in a soilless mode, simultaneously controls the environmental temperature and humidity and the variety proportion of feeds, and finally realizes that the survival rate and the euzoon (high in appearance quality) proportion of the hepialus larvae are higher than those of the hepialus larvae in the soil feeding method.
Finally, the soil-free cultivation method is difficult to find when stiff insects are selected due to stiff larvae in soil and other matrixes, so that the difficulty is completely solved, the stiff larvae can be found very simply after stiff larvae, and the operation of later-stage stiff insects selection of semi-finished products is facilitated.
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In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a photograph of a high quality muscardine insect cultivated in the present invention;
FIG. 2 is a photograph of stiff spot;
FIG. 3 is a photograph of a stiff worm with uneven color;
fig. 4 is a photograph of a small insect, a malformed insect, and a flat insect.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It will be apparent that the described embodiments are only some, but not all, embodiments of the invention. All other embodiments, based on the examples herein, which are within the scope of the invention as defined by the claims, will be within the scope of the invention as defined by the claims.
The appearance quality of the hepialus larva in the technical scheme of the invention comprises the aspects of whether the body of the hepialus larva is full, whether the color of the body is uniform, whether spots exist in the body and the like.
Stiff worms: and (3) successfully infecting the hepialus larva of the hirsutella sinensis, and under proper time and conditions, the hirsutella sinensis hyphae germinate in a large amount in the larva body, so that the larva dies and hardens to become stiff worms.
High-quality stiff worms: the appearance quality is good, the appearance is full, the color is milky white or light yellow, no obvious spots exist, and the stiff insects with uneven color are avoided, as shown in figure 1;
Stiff worm spot: namely, the appearance is full as a whole, the color is milky white or light yellow, and the surface of the insect body is provided with spots, small scars and other stiff insects, as shown in figure 2;
The color is uneven stiff worm: namely, the appearance is full, the color is milky white or light yellow, and stiff worms with large-area dark yellow/gray/black abnormal body colors are mixed, as shown in figure 3;
Small insects and malformed insects: i.e. the bodies are 4 years old and less, or stiff insects with the heads obviously bigger than the bodies appear, as shown in figure 4;
A shrunken insect: the body is stiff and flat, as shown in figure 4;
The raising method for improving the appearance quality of hepialus larvae, namely the raising method for the hepialus larvae in the high-age period, comprises the following steps of:
5-8-year-old hepialus larva is selected to be placed in a feeding box for feeding,
One hepialus larva is placed in each feeding box (namely single-bin feeding);
Placing a feed in the feeding box, wherein the feed comprises one or more of radix potentillae anserinae tubers, polygonum aviculare tubers, carrots and wild buckwheat tubers;
The feeding substrate is not placed in the feeding box.
More specifically, the invention completes the culture of hepialus larva in C-level purifying workshop, and is carried out under the conditions of 5-16 deg.c, 60-90% humidity and no light, and the used raising box and feed are strictly disinfected and used after bacteria inspection is qualified.
1. And (5) feeding larvae of 5 years old and above in a single bin.
2. And selecting radix potentillae anserinae tubers, polygonum aviculare tubers, carrots and wild buckwheat tubers as larva feeds.
3. One hepialus larva is placed in each breeding box, namely, a single-bin breeding method (the single-bin breeding box can be selected from the patent of the utility model) is adopted for soilless breeding, and the single-bin breeding can provide a relatively closed environment for each hepialus larva, so that the temperature and the humidity in any single-bin small space are relatively stable.
4. Within each feeding box, only feed and larvae were placed, without the addition of soil or other feeding substrates.
5. Each feed is specially proportioned, so that the humidity in a single-bin small space of each feed box is controlled through the moisture of the feed, the relative humidity in each single bin is ensured to be 60% -90%, and a proper environment is provided for the larva growth environment.
The feed proportioning condition is as follows: 25 g-30 g of feed.
Example 1
The raising method for improving the appearance quality of hepialus larvae comprises the following steps:
Selecting 5-year-old hepialus larvae to be placed in a feeding box for feeding, wherein one hepialus larva is placed in each feeding box;
The fodder is placed in the feeding box, and the fodder is radix Potentillae Anserinae root tuber 10g, herba Polygoni Perfoliati root tuber 10g and radix Dauci Sativae 10g. The feeding cassette does not house a feeding substrate.
The feeding conditions are as follows: the ambient temperature is 5-16 ℃, the humidity is 60-90%, and no light exists.
The used feeding boxes and feeds are strictly sterilized and used after the bacteria inspection is qualified.
The feed was changed every 10 days, and the excrement and feed residues of hepialus larvae in the feed box were cleaned, and after 30 days of incubation in a dark environment, the selection of stiff and dead insects was started, and then once every 10 days, and the selection was ended after the 4 th selection was completed to 60 days.
Experimental example 1
In a C-level purifying workshop, the environment temperature is 5-16 ℃, the humidity is 30-60%, and the used feeding boxes and feeds are strictly sterilized under the dark condition, and the bacteria inspection is qualified for use.
Selecting more than 5-year hepialus larvae which are already infected with hirsutella sinensis, wherein the selected hepialus larvae are the same batch of infected larvae, so that the infected rate is consistent;
Experimental group: only hepialus larva and feeds with different proportions are put into a layered single-bin feeding box for cultivation, and a plurality of groups of feeds are selected for proportion, so that soilless feeding is performed.
Control group: the method is characterized in that the plateau ecological soil is used as a feeding matrix, and hepialus larva, high-primordial soil and feed are put into a single-bin feeding box for cultivation. The experimental setup was as follows:
The selection of stiff and dead insects was started after 30 days of incubation in a dark environment, followed by a selection every 10 days, and ended after the 4 th selection was completed to 60 days, and the hepialus larvae survival and rigidification were counted, with the following results:
The results are shown in Table 1, the environmental temperature is 5-16 ℃, the humidity is 30% -60%, and the survival rate of the larva is lower in the aspect of the survival rate of the larva of hepialus cultivated in a single-bin soilless mode than in the aspect of the soil cultivation in a single bin.
In the aspect of feed samples, the survival rate of the larvae of the hepialus fed with the feeds matched with the silverweed cinquefoil, the polygonum perfoliatum and the carrot is highest, the survival rate of the larvae fed with the feeds matched with the carrot and the wild buckwheat is lowest, and the survival rate of the larvae fed with the feeds together containing the silverweed cinquefoil and the polygonum perfoliatum is higher than that of the larvae fed with the feeds singly containing the silverweed cinquefoil or the polygonum perfoliatum.
TABLE 2 quality Condition of stiff worms
As can be seen from Table 2, the environment temperature is 5-16 ℃, the humidity is 30% -60%, the number of stiff worms is 438 in the whole stiff worms of 8 experimental groups under the soilless single-bin culture condition, and the total number of stiff worms obtained by 8 control groups is 548 under the soil single-bin culture condition; in the aspect of high-quality stiff worms, under the soilless single-bin culture condition, the total number of the high-quality stiff worms is 368, the proportion of the high-quality stiff worms is 84.02%, and under the soil single-bin culture condition, the total number of the high-quality stiff worms is 408, and the proportion of the high-quality stiff worms is 74.54%.
Experimental example 2
Experimental example 2 differs from experimental example 1 in that the humidity is 60% -90%, and other experimental conditions are the same.
The settings of the experimental group and the control group were also the same as those of experimental example 1. The experimental results are as follows:
The result shows that under the conditions of the environmental temperature of 5-16 ℃ and the humidity of 60-90%, the single-bin soilless culture is obviously improved compared with the 30-60% humidity condition in terms of the survival rate of the hepialus larva, the single-bin soilless culture is not obviously improved compared with the 30-60% humidity condition, and the single-bin soilless culture is slightly higher than the single-bin soilless culture.
In terms of feed proportioning, three matched feeds of potentilla anserina, polygonum fagopyrum and golden buckwheat are used, the survival rate of the hepialus larva is highest, the survival rate of the two matched larvae of the carrot and the golden buckwheat is lowest, and when the potentilla anserina and the polygonum fagopyrum are contained in the feed together, the survival rate of the hepialus larva is higher than that of the single potentilla anserina or the polygonum fagopyrum.
The result shows that under the conditions that the ambient temperature is 5-16 ℃ and the humidity is 60-90%, the number of stiff worms is 534 in the whole experimental group, and the total number of stiff worms is 532 in the control group; in terms of high-quality stiff worms, the whole experimental group is high-quality stiff worms in 437, the high-quality stiff worms account for 81.84 percent, the total stiff worms in 385 of the experimental group account for 72.37 percent.
Experimental examples 1 and 2 show that the environment temperature is 5-16 ℃, the humidity is 60-90%, and the high-quality cordyceps sinensis stiff worms are produced in a good proportion by cultivating under the condition of no light and no culture medium; the feed ratio is selected to be the main feed of potentilla anserina and the polygonum fagopyrum, and the survival rate of the hepialus larva is better under the condition that the golden buckwheat/carrot is used as the auxiliary feed.
Experimental example 3
1. In a C-level purification workshop, the environment temperature is 5-16 ℃, the humidity is 60-90% and the environment is in a dark condition, and the used feeding boxes and feeds are strictly sterilized and used after the bacteria inspection is qualified;
2. selecting 5-year or more hepialus larvae which are not infected with hirsutella sinensis;
3. Selecting a plurality of groups of feed proportions, and carrying out soilless breeding, namely placing hepialus larvae and feeds with different proportions into a layered single-bin breeding box for breeding. Larval survival was observed after 60 days incubation in the absence of light.
The experimental setup was as follows:
Sequence number Feed proportioning Number of hepialus larva samples
1 5G of potentilla anserina, 5g of polygonum aviculare and 20g of carrot 100
2 5G of potentilla anserina, 10g of polygonum aviculare and 15g of carrot 100
3 10G of potentilla anserina, 5g of polygonum aviculare and 15g of carrot 100
4 15G of potentilla anserina, 5g of polygonum aviculare and 10g of carrot 100
5 5G of potentilla anserina, 15g of polygonum aviculare and 10g of carrot 100
6 10G of potentilla anserina, 10g of polygonum aviculare and 10g of carrot 100
7 10G of potentilla anserina, 15g of polygonum aviculare and 5g of carrot 100
8 Radix potentillae anserinae 15g, herba polygoni orientalis 10g, and radix Dauci Sativae 5g 100
9 5G of potentilla anserina, 5g of polygonum aviculare and 20g of wild buckwheat 100
10 5G of potentilla anserina, 10g of polygonum aviculare and 15g of wild buckwheat 100
11 10G of potentilla anserina, 5g of polygonum aviculare and 15g of wild buckwheat 100
12 15G of potentilla anserina, 5g of polygonum aviculare and 10g of wild buckwheat 100
13 5G of potentilla anserina, 15g of polygonum aviculare and 10g of wild buckwheat 100
14 Radix potentillae anserinae 10g, herba polygoni multiflori 10g, rhizoma Fagopyri Dibotryis 10g 100
15 10G of potentilla anserina, 15g of polygonum aviculare and 5g of golden buckwheat 100
16 Radix potentillae anserinae 15g, herba polygoni multiflori 10g, rhizoma Fagopyri Dibotryis 5g 100
TABLE 5 feeding of the resulting hepialus larvae
Sequence number Small and malformed insects Shrivelled insect Dead insects High quality hepialus larva
1 15 0 12 73
2 19 0 3 78
3 23 0 6 71
4 17 4 4 75
5 14 6 5 75
6 10 3 5 82
7 16 11 7 66
8 12 10 8 70
9 28 2 8 62
10 15 5 4 76
11 16 6 4 74
12 15 5 4 76
13 11 7 5 77
14 9 4 6 81
15 19 10 3 68
16 14 8 6 72
The high quality hepialus larval rates in table 5 were ranked, wherein,
High quality hepialus larva rate = high quality hepialus larva number/sample number x 100%;
The feed proportioning conditions corresponding to the sorted feed are shown in the following table:
Ordering of Feed proportioning High quality hepialus larva rate
1 10G of potentilla anserina, 10g of polygonum aviculare and 10g of carrot 82.0%
2 Radix potentillae anserinae 10g, herba polygoni multiflori 10g, rhizoma Fagopyri Dibotryis 10g 81.0%
3 5G of potentilla anserina, 10g of polygonum aviculare and 15g of carrot 78.0%
4 5G of potentilla anserina, 15g of polygonum aviculare and 10g of wild buckwheat 77.0%
5 5G of potentilla anserina, 10g of polygonum aviculare and 15g of wild buckwheat 76.0%
6 15G of potentilla anserina, 5g of polygonum aviculare and 10g of wild buckwheat 76.0%
7 15G of potentilla anserina, 5g of polygonum aviculare and 10g of carrot 75.0%
8 5G of potentilla anserina, 15g of polygonum aviculare and 10g of carrot 75.0%
9 10G of potentilla anserina, 5g of polygonum aviculare and 15g of wild buckwheat 74.0%
10 5G of potentilla anserina, 5g of polygonum aviculare and 20g of carrot 73.0%
11 Radix potentillae anserinae 15g, herba polygoni multiflori 10g, rhizoma Fagopyri Dibotryis 5g 72.0%
12 10G of potentilla anserina, 5g of polygonum aviculare and 15g of carrot 71.0%
13 Radix potentillae anserinae 15g, herba polygoni orientalis 10g, and radix Dauci Sativae 5g 70.0%
14 10G of potentilla anserina, 15g of polygonum aviculare and 5g of golden buckwheat 68.0%
15 10G of potentilla anserina, 15g of polygonum aviculare and 5g of carrot 66.0%
16 5G of potentilla anserina, 5g of polygonum aviculare and 20g of wild buckwheat 62.0%
According to the experimental example, uninfected hepialus larva is selected for experiment to determine the optimal feed, and according to the result, the optimal feed proportion is 10g of potentilla anserina, 10g of polygonum perfoliatum, 10g of carrot/golden buckwheat, and the recommended proportion is 5-10 g of potentilla anserina, 10-15 g of polygonum perfoliatum and 10-15 g of carrot/golden buckwheat by considering the actual filling condition and the raw material price condition in mass production.
The foregoing is merely illustrative of the present invention, and the present invention is not limited thereto, and any person skilled in the art will readily recognize that variations or substitutions are within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims (3)

1. A feeding method for improving the appearance quality of hepialus larvae, characterized in that the method comprises the following steps: raising hepialus larva at 5-16 deg.c and 60-90% humidity and no light,
The hepialus larva with 5-8 years old is selected to be put into a feeding box for feeding,
One hepialus larva is placed in each feeding box;
The fodder is placed in the raising box, the fodder is radix potentillae anserinae root tuber, polygonum mulberosum root tuber and carrot, the weight ratio of radix potentillae anserinae root tuber, polygonum mulberosum root tuber and carrot is 1-2:1-3:1-3; or alternatively
The feed is radix potentillae anserinae, rhizoma polygoni cyrtomii and rhizoma Fagopyri Dibotryis tuber; the weight ratio of the radix potentillae anserinae tubers, the polygonum capitatum tubers and the wild buckwheat tubers is 1-2:1-3:1-3; the feed placed in the feed box is replaced every 8-12 days;
The feeding matrix is not placed in the feeding box;
The culture time of hepialus larva is 40-60 days.
2. A feeding method for improving the appearance quality of hepialus larvae according to claim 1, wherein the total weight of the feed placed in the feeding box is 20-30g.
3. The raising method for hepialus larva appearance quality improvement according to claim 1, wherein after 30 days of hepialus larva cultivation, stiff and dead insects are selected, and thereafter, stiff and dead insects are selected every 10 days until the hepialus larva cultivation is completed for 60 days, and the cultivation is ended after completion of 4 th selection.
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