CN117646054A - Process for producing, separating and purifying bone polypeptide for treating arthritis - Google Patents
Process for producing, separating and purifying bone polypeptide for treating arthritis Download PDFInfo
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- CN117646054A CN117646054A CN202410117641.9A CN202410117641A CN117646054A CN 117646054 A CN117646054 A CN 117646054A CN 202410117641 A CN202410117641 A CN 202410117641A CN 117646054 A CN117646054 A CN 117646054A
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 24
- 210000000988 bone and bone Anatomy 0.000 title claims abstract description 22
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 22
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 22
- 206010003246 arthritis Diseases 0.000 title claims abstract description 21
- 230000008569 process Effects 0.000 title claims description 22
- 210000000845 cartilage Anatomy 0.000 claims abstract description 59
- 239000000706 filtrate Substances 0.000 claims abstract description 22
- 230000001680 brushing effect Effects 0.000 claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 238000000926 separation method Methods 0.000 claims abstract description 14
- 239000000463 material Substances 0.000 claims abstract description 13
- 102000004190 Enzymes Human genes 0.000 claims abstract description 12
- 108090000790 Enzymes Proteins 0.000 claims abstract description 12
- 229940088598 enzyme Drugs 0.000 claims abstract description 12
- 241001465754 Metazoa Species 0.000 claims abstract description 10
- 230000009849 deactivation Effects 0.000 claims abstract description 9
- 239000004365 Protease Substances 0.000 claims abstract description 8
- 238000011010 flushing procedure Methods 0.000 claims abstract description 8
- 238000004519 manufacturing process Methods 0.000 claims abstract description 8
- 238000010411 cooking Methods 0.000 claims abstract description 7
- 238000010979 pH adjustment Methods 0.000 claims abstract description 7
- 238000001914 filtration Methods 0.000 claims abstract description 6
- 108090000526 Papain Proteins 0.000 claims abstract description 4
- 108091005804 Peptidases Proteins 0.000 claims abstract description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 4
- 238000001035 drying Methods 0.000 claims abstract description 4
- 229940055729 papain Drugs 0.000 claims abstract description 4
- 235000019834 papain Nutrition 0.000 claims abstract description 4
- 239000000843 powder Substances 0.000 claims abstract description 4
- 235000019419 proteases Nutrition 0.000 claims abstract description 4
- 238000001694 spray drying Methods 0.000 claims abstract description 4
- 150000001875 compounds Chemical class 0.000 claims abstract description 3
- 230000008093 supporting effect Effects 0.000 claims description 41
- 230000007246 mechanism Effects 0.000 claims description 33
- 230000005540 biological transmission Effects 0.000 claims description 24
- 239000012528 membrane Substances 0.000 claims description 12
- 238000009826 distribution Methods 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000005507 spraying Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 6
- 238000000746 purification Methods 0.000 abstract description 5
- 239000000047 product Substances 0.000 abstract description 4
- 235000013372 meat Nutrition 0.000 description 9
- 239000004519 grease Substances 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 3
- 102100027995 Collagenase 3 Human genes 0.000 description 2
- 108050005238 Collagenase 3 Proteins 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 201000008482 osteoarthritis Diseases 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 102000003777 Interleukin-1 beta Human genes 0.000 description 1
- 108090000193 Interleukin-1 beta Proteins 0.000 description 1
- 102000003814 Interleukin-10 Human genes 0.000 description 1
- 108090000174 Interleukin-10 Proteins 0.000 description 1
- 108090001007 Interleukin-8 Proteins 0.000 description 1
- 102000004890 Interleukin-8 Human genes 0.000 description 1
- 102000002274 Matrix Metalloproteinases Human genes 0.000 description 1
- 108010000684 Matrix Metalloproteinases Proteins 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 1
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- 210000001188 articular cartilage Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/01—Hydrolysed proteins; Derivatives thereof
- A61K38/012—Hydrolysed proteins; Derivatives thereof from animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/02—Cleaning by the force of jets or sprays
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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- Pharmacology & Pharmacy (AREA)
- Genetics & Genomics (AREA)
- Animal Behavior & Ethology (AREA)
- Biochemistry (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Water Supply & Treatment (AREA)
- Epidemiology (AREA)
- Analytical Chemistry (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Biophysics (AREA)
- Rheumatology (AREA)
- Physical Education & Sports Medicine (AREA)
- Biotechnology (AREA)
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- General Engineering & Computer Science (AREA)
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- Peptides Or Proteins (AREA)
Abstract
The invention discloses a bone polypeptide production separation and purification process for treating arthritis, which comprises the following steps: step one, preparing materials; step two, curing, which comprises the following steps: placing animal cartilage in a preset temperature environment and an air pressure environment for cooking; step three, residue separation, which comprises: brushing the cured cartilage by a preset multi-brush-roller staggered brushing device; step four, high-pressure flushing, which comprises the following steps: a plurality of fine high-pressure water columns are distributed to flush the brushed cartilage; step five, PH adjustment; step six, enzymolysis, it includes: adding compound protease and papain into an enzymolysis container, and keeping the temperature at a first temperature for a period of time T2; step seven, enzyme deactivation treatment; step eight, filtering; step nine, drying and producing, which comprises the following steps: spray drying the concentrated final filtrate to obtain cartilage peptide powder. The method has the effect of improving the separation convenience and the processing efficiency of the peptide product.
Description
Technical Field
The application relates to the technical field of bone polypeptide separation, in particular to a bone polypeptide production separation and purification process for treating arthritis.
Background
Arthritis, an inflammatory disease of the joints of the human body and surrounding tissues, and the common type of arthritis is osteoarthritis; osteoarthritis can exercise strong muscles and maintain healthy weight loss symptoms by exercise and healthy diet.
At present, animal experiments show that the joint peptide can down regulate MMP-13 activity in arthritic rats to a certain extent (MMP-13 is one of matrix metalloproteinases, can degrade collagen in articular cartilage and aggravate joint abrasion), shows good joint protection effect, can obviously reduce IL-8, IL-1 beta, IL-10 and TNF-alpha in blood, and shows that the joint peptide has good anti-inflammatory activity.
Currently, peptide products are produced by separation from animal cartilage, however, residual meat and fat residues on animal cartilage are removed. At present, residual meat and grease are manually removed by workers, so that the peptide product separation process is relatively time-consuming and labor-consuming, and a new technical scheme is provided.
Disclosure of Invention
In order to improve the separation convenience and the processing efficiency of peptide products, the application provides a bone polypeptide production separation and purification process for treating arthritis.
The application provides a bone polypeptide production separation purification process for treating arthritis, which adopts the following technical scheme:
a process for producing, separating and purifying bone polypeptide for treating arthritis, which comprises the following steps:
step one, preparing materials, which comprises the following steps: taking animal cartilage conforming to a production plan;
step two, curing, which comprises the following steps: placing animal cartilage in a preset temperature environment and an air pressure environment for cooking, wherein the cooking time is T1;
step three, residue separation, which comprises: brushing the cured cartilage by a preset multi-brush-roller staggered brushing device;
step four, high-pressure flushing, which comprises the following steps: a plurality of fine high-pressure water columns are distributed to flush the brushed cartilage;
step five, PH adjustment, which comprises: placing the washed cartilage in an enzymolysis container, adding water according with the formula amount, detecting the PH value in real time, and adding a preselected acid-base regulator for PH adjustment until the PH value meets the standard value;
step six, enzymolysis, it includes: adding compound protease and papain into an enzymolysis container, and keeping the temperature at a first temperature for a period of time T2;
step seven, enzyme deactivation treatment, which comprises the following steps: heating to a second temperature to deactivate enzyme for a period of time T3; the second temperature is greater than the first temperature;
step eight, filtering, which comprises the following steps: the solution obtained after the enzyme deactivation treatment is subjected to an inorganic membrane to obtain primary filtrate, the primary filtrate is subjected to an organic membrane to obtain final filtrate, and the final filtrate is evaporated and concentrated;
step nine, drying and producing, which comprises the following steps: spray drying the concentrated final filtrate to obtain cartilage peptide powder.
Optionally, the multi-brush roller staggered brushing device comprises:
the upper part of the shell is provided with a feed inlet, and the lower part of the shell is overhead by a stay bar and is provided with a discharge outlet;
the staggered roller sets are arranged in the shell and are in multiple groups and vertically distributed;
a reciprocating supporting plate which is positioned below each staggered roller group and is provided with brush hair at the upper part;
the driving mechanism is used for driving the staggered roller groups to operate and driving the reciprocating supporting plates to transversely reciprocate;
the staggered roller set comprises two parallel roller bodies, wherein the roller bodies are rotationally connected to the machine shell, bristles are arranged on the outer wall of the roller bodies, the two roller bodies of the same staggered roller set are identical in rotation direction, the bristles are staggered with each other, and cartilage falls between the two roller bodies of the same staggered roller set;
the reciprocating supporting plate is also provided with a through hole for cartilage to pass through;
the driving mechanism enables the reciprocating supporting plate to reciprocate at a first movable distance, and then enables the reciprocating supporting plate to reciprocate at a second movable distance, and different movable distances are circularly alternated; when the reciprocating supporting plate reciprocates in the second movement, the through hole moves to a position between the two staggered roller groups at least once and stays for a period of T3.
Optionally, the bristle density on the staggered roller set of the next layer is greater than the bristle density on the staggered roller set of the previous layer, and the bristle softness on the staggered roller set of the next layer is greater than the bristle softness on the staggered roller set of the previous layer.
Optionally, the driving mechanism comprises a first motor, a second motor, a belt transmission mechanism and a reciprocating transmission mechanism;
at least one first motor drives the staggered roller set to operate through a belt transmission mechanism;
one of the staggered roller sets is matched with two second motors, the two second motors are respectively positioned at two ends of the reciprocating supporting plate, and the second motors drive the reciprocating supporting plate to move through a reciprocating transmission mechanism;
the reciprocating transmission mechanism comprises an incomplete gear, an elliptical ring and a connecting rod, one end of the connecting rod is fixed on an end plate of the reciprocating supporting plate, and the other end of the connecting rod is fixed on the outer end of the elliptical ring; the upper inner wall of the elliptical ring is respectively provided with a rack, the incomplete gear is positioned in the elliptical ring and is alternately meshed with the racks on the upper and lower inner walls of the elliptical ring when rotating, and the output shaft of the second motor is linked with the incomplete gear;
the reciprocating supporting plate is connected to the frame in a sliding manner, and the diameters and the numbers of teeth of the incomplete gears at the two ends of the reciprocating supporting plate are different.
Optionally, two downward flitch of slope are provided with in the feed inlet both sides of casing, two the lower extreme of flitch is close to each other, and two the direction of arranging of flitch is on a parallel with the direction of arranging of two roll bodies of same staggered roller group.
Optionally, a mounting frame is arranged below the lowest reciprocating supporting plate, two rows of vertical brush rollers are rotationally connected to the mounting frame, the distribution directions of a plurality of brush rollers in the same row are perpendicular to the roller bodies, and a dead zone between the two rows of brush rollers is opposite to the position between the two roller bodies of a certain group of staggered roller groups; a gear transmission mechanism and a third motor are arranged in the shell, and the third motor drives each brush roller to rotate through the gear transmission mechanism;
lifting plates are arranged between the two rows of brush rollers, a plurality of vertical positioning columns are arranged on the upper portions of the lifting plates, and an electric cylinder is arranged on the lower portions of the lifting plates.
Optionally, the lower part of mounting bracket is fixed with the riser, the riser is two and is located the both sides of lifter plate's lift route, install a plurality of shower nozzles that are used for spraying high-pressure water column on the riser, and from the top down has at least a set of shower nozzle slope downwards, a set of shower nozzle level, a set of shower nozzle slope upwards on every riser.
Optionally, the casing inner wall is provided with the spacing, the spacing is a plurality of and is located respectively under each roll body, the length of spacing extends along the length of roll body and separates with roll body and reciprocal fagging.
Optionally, the solution obtained after the enzyme deactivation treatment is filtered through an inorganic membrane of 0.22 mu m to obtain a primary filtrate, and the primary filtrate is filtered through an organic membrane of 2000 daltons to obtain a final filtrate.
Optionally, the first temperature comprises 50 ℃ and the second temperature comprises 95 ℃.
In summary, the present application includes at least one of the following beneficial technical effects:
1) The cartilage is relatively easier to separate bones and meat after being steamed, so that fat and residual meat on the cartilage can be conveniently and effectively removed;
2) The multi-brush-roller staggered brushing device is adopted to brush the cooked cartilage, then the high-pressure water column is used for flushing, the process of manually removing residual meat and grease is not needed, the labor is saved, the efficiency is relatively high, the cleaning effect is relatively stable, the raw material purity is higher, and the amount of impurity polypeptide generated after enzymolysis is less;
3) Filtering with inorganic membrane, removing unwanted macromolecular components, and retaining desired target components;
4) And the optimized enzymolysis process ensures that target molecules are released to the greatest extent, the yield is high, and the active ingredients are high.
Drawings
FIG. 1 is a schematic flow chart of the present application;
FIG. 2 is a schematic view of a multi-brush staggered brushing apparatus and a high pressure flushing configuration of the present application;
fig. 3 is a schematic diagram of the control structure of the present application.
Reference numerals illustrate: 1. a housing; 11. a limiting frame; 12. a material plate; 2. a staggered set of rollers; 21. a roller body; 3. reciprocating supporting plates; 4. a first motor; 5. a second motor; 6. a controller; 7. a reciprocating transmission mechanism; 71. an incomplete gear; 72. an elliptical ring; 73. a connecting rod; 8. a mounting frame; 81. a brush roller; 82. a gear transmission mechanism; 83. a third motor; 84. a riser; 85. a spray head; 91. a lifting plate; 92. positioning columns; 93. and (5) an electric cylinder.
Detailed Description
The present application is described in further detail below in conjunction with figures 1-3.
The embodiment of the application discloses a bone polypeptide production separation and purification process for treating arthritis.
Referring to fig. 1, the process for producing, isolating and purifying bone polypeptides for treating arthritis comprises:
step one, preparing materials, which comprises the following steps: and taking animal cartilage conforming to the production plan.
Step two, curing, which comprises the following steps: the animal cartilage is steamed in a preset temperature environment and an air pressure environment for a time period of T1.
The steps can be realized by a high-temperature high-pressure cooking pot, and the cooking time length T1 can be 60-120 min.
Step three, residue separation, which comprises: brushing the cured cartilage by a preset multi-brush-roller staggered brushing device.
Step four, high-pressure flushing, which comprises the following steps: and a plurality of fine high-pressure water columns are distributed to flush the brushed cartilage.
Step five, PH adjustment, which comprises: placing the washed cartilage in an enzymolysis container, adding water (for example, 4 times of cartilage) according with the formula amount, detecting the PH value in real time, and adding a preselected acid-base regulator for PH adjustment until the PH value meets the standard value.
Step six, enzymolysis, it includes: the composite protease and papain are added into an enzymolysis vessel, and the enzymolysis vessel is kept at a first temperature (such as 50 ℃) for a period of time (such as 30 minutes).
Step seven, enzyme deactivation treatment, which comprises the following steps: heating to a second temperature (95 ℃ for example) to deactivate enzyme for a period of time T3 (30 min for example); the second temperature is greater than the first temperature.
Step eight, filtering, which comprises the following steps: the solution obtained after the enzyme deactivation treatment is filtered by an inorganic membrane of 0.22 mu m to obtain primary filtrate, the primary filtrate is filtered by an organic membrane of 2000 daltons to obtain final filtrate, and the final filtrate is evaporated and concentrated.
Step nine, drying and producing, which comprises the following steps: spray drying the concentrated final filtrate to obtain cartilage peptide powder.
According to the above arrangement, the method comprises:
1) The cartilage is relatively easier to separate bones and meat after being steamed, so that fat and residual meat on the cartilage can be conveniently and effectively removed;
2) The multi-brush-roller staggered brushing device is adopted to brush the cooked cartilage, then the high-pressure water column is used for flushing, the process of manually removing residual meat and grease is not needed, the labor is saved, the efficiency is relatively high, the cleaning effect is relatively stable, the raw material purity is higher, and the amount of impurity polypeptide generated after enzymolysis is less;
3) Filtering with inorganic membrane, removing unwanted macromolecular components, and retaining desired target components;
4) And the optimized enzymolysis process ensures that target molecules are released to the greatest extent, the yield is high, and the active ingredients are high.
Referring to fig. 2, in one embodiment of the present application, the multi-brush roller interlacing and brushing device includes a housing 1, an interlacing roller set 2, a reciprocating stay 3, and a driving mechanism.
Wherein, the shell 1 can be made of alloy sheet, is a vertical long box structure and is provided with a feed inlet at the upper part, and the cooked cartilage is directly sent into the shell 1 in a toppling way.
The staggered roller sets 2 have a plurality of groups, in this embodiment, two groups are taken as an example, and the two groups of staggered roller sets are vertically distributed in the casing 1. The staggered roller set 2 comprises two parallel roller bodies 21, and the roller bodies 21 are horizontally arranged and connected with a bearing seat pre-fixed on the inner wall of the shell 1 through a rotating shaft so as to realize rotating connection; the outer wall of the roller body 21 is provided with bristles, and the bristles of the two roller bodies 21 of the same staggered roller group 2 are mutually staggered. Cartilage entering from the feed inlet of the casing 1 falls between the two roller bodies 21, and the rotation directions of the two roller bodies 21 are the same, so that the cartilage placed between the two roller bodies 21 is brushed by bristles in two opposite staggered movement directions, and even the cartilage is driven to roll between the two roller bodies 21, so that the residual meat and grease on the cartilage are removed.
The reciprocating supporting plate 3 is positioned below each group of staggered roller groups 2, and the upper parts of the reciprocating supporting plate are also fixed with bristles; the side part of the reciprocating supporting plate 3 is horizontally connected to the inner wall of the shell 1 in a sliding way, and moves back and forth when the staggered roller set 2 runs, so that on one hand, the brush hair is utilized to jack up cartilage, and the supporting effect is achieved; on the other hand, when cartilage is brushed again by the bristles, that is, when cartilage is placed between the two roller bodies 21, the cartilage is brushed by the bristles in all directions, thereby improving the brushing effect.
The reciprocating supporting plate 3 is also provided with a through hole for the cartilage to pass through. The reciprocating supporting plate 3 has two movable distances, the first movable distance is relatively smaller, and at the moment, one section of the reciprocating supporting plate 3 with bristles is always arranged in the middle area of the staggered roller set 2, namely, the reciprocating supporting plate has supporting and brushing functions; the second moving distance is relatively large, and at this time, the section of the reciprocating stay plate 3 with the through hole can move to the middle area of the staggered roller set, namely, the cartilage is not blocked from falling any more, so that the cartilage enters the next stage of treatment structure.
Referring to fig. 2 and 3, the above-mentioned staggered roller set 2 and reciprocating stay plate 3 are driven by a driving mechanism, and the driving mechanism includes: a first motor 4, a second motor 5, a belt transmission mechanism and a reciprocating transmission mechanism 7.
The belt transmission mechanism comprises a belt pulley and a belt, wherein the belt pulley is fixed on the rotating shaft of the roller body 21 and the output shaft of the first motor 4, and the belt is arranged on a plurality of belt pulleys so that the first motor 4 can drive the roller body 21 to rotate. In order to save cost, the belt pulley on the output shaft of the first motor 4 may be a double-grooved pulley, so that two belts can be utilized to drive two roller bodies 21 of the same staggered roller set 2 to rotate; even the double sheave may be replaced with a four sheave if necessary.
The reciprocating transmission mechanism 7 comprises an incomplete gear 71, an elliptical ring 72 and a connecting rod 73, one end of the connecting rod 73 is fixed at one end of the reciprocating supporting plate 3, and the other end is fixed at the outer end of the elliptical ring 72; the elliptical ring 72 is horizontally arranged, and racks are respectively formed on the upper and lower inner walls of the elliptical ring 72; the incomplete gear 71 is located in the elliptical ring 72 and alternately engages racks of the upper and lower inner walls of the elliptical ring 72 when rotated. The second motor 5 is fixed to the rotation shaft of the incomplete gear 71 through a coupling.
The two ends of the reciprocating supporting plate 3 are respectively provided with a group of reciprocating transmission mechanisms 7, and are respectively matched with a second motor 5. The diameters and the number of teeth of the incomplete gears 71 at the two ends of the same reciprocating supporting plate 3 are different, and the incomplete gears are one by one so as to meet the use requirements of the first movable distance and the second movable distance.
In the use process, the second motor 5 at one side of the small incomplete gear 71 actively rotates to brush the brush hair on the reciprocating supporting plate 3 to brush cartilage; brushing for a certain period of time, such as: after 15 min; the second motor 5 on one side of the large incomplete gear 71 actively rotates, so that the through hole on the reciprocating supporting plate 3 moves between the two roller bodies 21, and cartilage can drop downwards.
Referring to fig. 2, in order to prevent cartilage from being inadvertently moved between the two roller bodies 21 by the bristles of the roller bodies 21, a plurality of limiting frames 11 are fixed to the inner wall of the housing 1, the limiting frames 11 are respectively located right below the respective roller bodies 21, and the length of the limiting frames 11 extends along the length of the roller bodies 21 and is separated from the roller bodies 21 and the reciprocating stay plates 3.
According to the above arrangement, cartilage can be blocked by the stopper 11.
Preferably, the bristle density of the staggered roller set 2 of the lower layer is greater than the bristle density of the staggered roller set 2 of the upper layer, and the bristle softness of the staggered roller set 2 of the lower layer is greater than the bristle softness of the staggered roller set 2 of the upper layer. This arrangement is to enhance the brushing effect.
Referring to fig. 2, two inclined downward material plates 12 are welded and fixed at two sides of a feed inlet of the casing 1, the lower ends of the two material plates 12 are close to each other, and the arrangement direction of the two material plates 12 is parallel to the arrangement direction of two roller bodies 21 of the same staggered roller set 2. The existence of the material plate 12 can guide cartilage to slide between the roller bodies 21 on one hand, and can prevent the probability that the brush hair which is thrown when the roller bodies 21 rotate plays juice, residues and the like from the material inlet on the other hand.
In this embodiment, one side of the casing 1 is designed to be an open structure and a side door is hinged by a hinge, which is provided to facilitate the user to wash the inside of the casing 1.
In one embodiment, referring to the figure, a mounting frame 8 is arranged below the lowermost reciprocating supporting plate 3, two rows of vertical brush rollers 81 are rotatably connected to the mounting frame 8, the distribution direction of a plurality of brush rollers 81 in the same row is perpendicular to the roller bodies 21, and a dead zone between the two rows of brush rollers 81 is opposite to a space between the two roller bodies 21 of a certain group of staggered roller groups 2; a gear transmission mechanism 82 and a third motor 83 are arranged in the machine shell 1, and the third motor 83 drives each brush roller 81 to rotate through the gear transmission mechanism 82.
The third motor 83 is mounted on a certain frame in the casing 1, and the gear transmission mechanism 82 includes gears fixed on the rotation shaft of the brush roller 81, and gears fixed on the output shaft of the third motor 83, and the gears are engaged with each other.
A lifting plate 91 is arranged between the two rows of brush rollers 81, a plurality of vertical positioning columns 92 are fixed on the upper portion of the lifting plate 91, and an electric cylinder 93 is arranged on the lower portion of the lifting plate. The lifter plate 91 has a grid structure.
Cartilage separated from the staggered roller set 2 falls between the positioning columns 92 on the lifting plate 91 and is suspended and fixed under the action of the positioning columns 92, and in the process, each brush roller 81 rapidly rotates to brush the cartilage again; and in the process, the electric cylinder 93 is gradually shortened to drive the cartilage to slowly move downwards.
Referring to fig. 2, a riser 84 is fixed to the lower portion of the mounting frame 8, two risers 84 are located on both sides of the lifting path of the lifting plate 91, a plurality of nozzles 85 for spraying high-pressure water columns are installed on the riser 84, and at least one group of nozzles are inclined downward, one group of nozzles are horizontal, and one group of nozzles are inclined upward from top to bottom on each riser.
According to the above arrangement, the cartilage can be washed by the high-pressure water column during the descent of the electric cylinder 93.
Referring to fig. 2, the lower part of the casing 1 is overhead by supporting feet and is provided with a discharge port; the discharge hole can be used for the lifting plate 91 to pass through, and the cylinder body of the electric cylinder 93 is fixed on the supporting feet at the bottom of the shell 1 by a transverse frame.
It will be appreciated that a great deal of residue is produced in brushing the cartilage using the above structure, so that a plurality of flat nozzles facing the roller structure can be further arranged inside the housing 1, and the flat nozzles are connected with a water pump to wash the cartilage during the rotation of the roller structure.
The above process produces more waste water, in order to reduce the influence on discharging and high-pressure flushing, the inner bottom of the casing 1 is inclined towards the side by taking two rows of brush rollers 81 as dividing lines, and water discharging ports are opened at two sides of the bottom of the casing 1.
The discharged waste water is filtered, and the residue is recovered for use as feed, biological fertilizer and the like.
In order to facilitate the automatic completion of the cartilage cleaning work, the controller 6 is configured for the above structure, and the controller 6 is mounted on the outer wall of the casing 1 and electrically connected to each electrical appliance, so as to achieve the purpose of use.
The foregoing are all preferred embodiments of the present application, and are not intended to limit the scope of the present application in any way, therefore: all equivalent changes in structure, shape and principle of this application should be covered in the protection scope of this application.
Claims (10)
1. A process for producing, separating and purifying bone polypeptide for treating arthritis, which is characterized by comprising the following steps:
step one, preparing materials, which comprises the following steps: taking animal cartilage conforming to a production plan;
step two, curing, which comprises the following steps: placing animal cartilage in a preset temperature environment and an air pressure environment for cooking, wherein the cooking time is T1;
step three, residue separation, which comprises: brushing the cured cartilage by a preset multi-brush-roller staggered brushing device;
step four, high-pressure flushing, which comprises the following steps: a plurality of fine high-pressure water columns are distributed to flush the brushed cartilage;
step five, PH adjustment, which comprises: placing the washed cartilage in an enzymolysis container, adding water according with the formula amount, detecting the PH value in real time, and adding a preselected acid-base regulator for PH adjustment until the PH value meets the standard value;
step six, enzymolysis, it includes: adding compound protease and papain into an enzymolysis container, and keeping the temperature at a first temperature for a period of time T2;
step seven, enzyme deactivation treatment, which comprises the following steps: heating to a second temperature to deactivate enzyme for a period of time T3; the second temperature is greater than the first temperature;
step eight, filtering, which comprises the following steps: the solution obtained after the enzyme deactivation treatment is subjected to an inorganic membrane to obtain primary filtrate, the primary filtrate is subjected to an organic membrane to obtain final filtrate, and the final filtrate is evaporated and concentrated;
step nine, drying and producing, which comprises the following steps: spray drying the concentrated final filtrate to obtain cartilage peptide powder.
2. The process for producing, separating and purifying a bone polypeptide for treating arthritis according to claim 1, wherein the multi-brush roll interlacing brushing device comprises:
the upper part of the shell (1) is provided with a feed inlet, and the lower part of the shell is overhead by a stay bar and is provided with a discharge outlet;
the staggered roller sets (2) are arranged in the shell (1) and are in multiple groups and vertically distributed;
a reciprocating supporting plate (3) which is positioned below each staggered roller group (2) and is provided with brush hair at the upper part;
the driving mechanism is used for driving the staggered roller groups (2) to operate and driving the reciprocating supporting plates (3) to transversely reciprocate;
the staggered roller set (2) comprises two parallel roller bodies (21), the roller bodies (21) are rotatably connected to the machine shell (1), bristles are arranged on the outer wall of the roller bodies, the two roller bodies (21) of the same staggered roller set (2) are identical in rotation direction, the bristles are staggered, and cartilage falls between the two roller bodies (21) of the same staggered roller set (2);
the reciprocating supporting plate (3) is also provided with a through hole for cartilage to pass through;
the driving mechanism enables the reciprocating supporting plate (3) to reciprocate at a first movable distance, and then enables the reciprocating supporting plate (3) to reciprocate at a second movable distance, and different movable distances are circularly alternated; when the reciprocating supporting plate (3) reciprocates in a second movement, the through hole moves to a position between the two staggered roller groups (2) at least once and stays for a period of T3.
3. The process for producing, separating and purifying a bone polypeptide for treating arthritis according to claim 2, wherein: the bristle density on the staggered roller set (2) of the next layer is greater than the bristle density on the staggered roller set (2) of the previous layer, and the bristle softness on the staggered roller set (2) of the next layer is greater than the bristle softness on the staggered roller set (2) of the previous layer.
4. The process for producing, separating and purifying a bone polypeptide for treating arthritis according to claim 2, wherein: the driving mechanism comprises a first motor (4), a second motor (5), a belt transmission mechanism and a reciprocating transmission mechanism (7);
at least one first motor (4) drives the staggered roller set (2) to operate through a belt transmission mechanism;
one staggered roller set (2) is matched with two second motors (5), the two second motors (5) are respectively positioned at two ends of the reciprocating supporting plate (3), and the second motors (5) drive the reciprocating supporting plate (3) to move through a reciprocating transmission mechanism (7);
the reciprocating transmission mechanism (7) comprises an incomplete gear (71), an elliptical ring (72) and a connecting rod (73), one end of the connecting rod (73) is fixed on an end plate of the reciprocating supporting plate (3), and the other end of the connecting rod is fixed on the outer end of the elliptical ring (72); the upper inner wall of the elliptical ring (72) is respectively provided with a rack, the incomplete gear (71) is positioned in the elliptical ring (72) and is alternately meshed with the racks on the upper inner wall and the lower inner wall of the elliptical ring (72) when rotating, and the output shaft of the second motor (5) is linked with the incomplete gear (71);
the reciprocating supporting plate (3) is connected to the frame in a sliding way, and the diameters and the tooth numbers of the incomplete gears (71) at the two ends are different.
5. The process for producing, separating and purifying a bone polypeptide for treating arthritis according to claim 2, wherein: two obliquely downward material plates (12) are arranged on two sides of a feed inlet of the shell (1), the lower ends of the two material plates (12) are close to each other, and the arrangement directions of the two material plates (12) are parallel to the arrangement directions of two roller bodies (21) of the same staggered roller group (2).
6. The process for producing, separating and purifying a bone polypeptide for treating arthritis according to claim 2, wherein: the lower part of the lowest reciprocating supporting plate (3) is provided with a mounting frame (8), two rows of vertical brush rollers (81) are rotationally connected to the mounting frame (8), the distribution direction of the plurality of brush rollers (81) in the same row is perpendicular to the roller bodies (21), and a dead zone between the two rows of brush rollers (81) is opposite to the space between the two roller bodies (21) of a certain group of staggered roller groups (2); a gear transmission mechanism (82) and a third motor (83) are arranged in the shell (1), and the third motor (83) drives each brush roller (81) to rotate through the gear transmission mechanism (82);
lifting plates (91) are arranged between the two rows of brush rollers (81), a plurality of vertical positioning columns (92) are arranged on the upper portions of the lifting plates (91), and electric cylinders (93) are arranged on the lower portions of the lifting plates.
7. The process for producing, isolating and purifying a bone polypeptide for treating arthritis according to claim 6, wherein: the lower part of mounting bracket (8) is fixed with riser (84), riser (84) are two and are located the both sides of the lift route of lifter plate (91), install a plurality of shower nozzles (85) that are used for spraying high-pressure water column on riser (84), and from the top down on every riser (84) have at least a set of shower nozzle (85) slope down, a set of shower nozzle (85) level, a set of shower nozzle (85) slope up.
8. The process for producing, isolating and purifying a bone polypeptide for treating arthritis according to claim 3, wherein: the utility model discloses a roller machine, including casing (1), roller body (21), casing (1) inner wall is provided with spacing (11), spacing (11) are a plurality of and are located respectively under each roller body (21), the length of spacing (11) extends along the length of roller body (21) and separates with roller body (21) and reciprocal fagging (3).
9. The process for producing, isolating and purifying a bone polypeptide for treating arthritis according to claim 1, wherein: the solution obtained after the enzyme deactivation treatment is filtered by an inorganic membrane with the thickness of 0.22 mu m to obtain primary filtrate, and the primary filtrate is filtered by an organic membrane with the thickness of 2000 daltons to obtain final filtrate.
10. The process for producing, isolating and purifying a bone polypeptide for treating arthritis according to claim 1, wherein: the first temperature comprises 50 ℃ and the second temperature comprises 95 ℃.
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA506108A (en) * | 1954-09-28 | Krijgsman Centinus | Separating method and apparatus | |
| KR20030046810A (en) * | 2001-12-06 | 2003-06-18 | 은삼제약 주식회사 | Pharmaceutical composition for prevention or treatment of arthritis containing degraded chondroitin sulfate |
| CN104450841A (en) * | 2013-06-27 | 2015-03-25 | 青岛贝尔特生物科技有限公司 | Method for producing chondroitin sulfate and co-producing hydrolyzed collagen from fish cartilage |
| CN206851916U (en) * | 2017-06-22 | 2018-01-09 | 胜田(福清)食品有限公司 | A kind of cleaning sea cucumber machine |
| CN111134256A (en) * | 2018-11-06 | 2020-05-12 | 湖南天劲制药有限责任公司 | Bone peptide beverage formula with blood replenishing effect and preparation method thereof |
| CN112494633A (en) * | 2020-11-30 | 2021-03-16 | 湖南天劲制药有限责任公司 | Bone peptide oral liquid production preparation system |
| CN112956583A (en) * | 2021-02-24 | 2021-06-15 | 北京金华雅轩健康科技有限公司 | Extraction process of fish collagen peptide |
| CN116590370A (en) * | 2023-04-24 | 2023-08-15 | 安徽盛美诺生物技术有限公司 | Cartilage peptide capable of promoting chondrocyte growth and preparation method thereof |
-
2024
- 2024-01-29 CN CN202410117641.9A patent/CN117646054B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA506108A (en) * | 1954-09-28 | Krijgsman Centinus | Separating method and apparatus | |
| KR20030046810A (en) * | 2001-12-06 | 2003-06-18 | 은삼제약 주식회사 | Pharmaceutical composition for prevention or treatment of arthritis containing degraded chondroitin sulfate |
| CN104450841A (en) * | 2013-06-27 | 2015-03-25 | 青岛贝尔特生物科技有限公司 | Method for producing chondroitin sulfate and co-producing hydrolyzed collagen from fish cartilage |
| CN206851916U (en) * | 2017-06-22 | 2018-01-09 | 胜田(福清)食品有限公司 | A kind of cleaning sea cucumber machine |
| CN111134256A (en) * | 2018-11-06 | 2020-05-12 | 湖南天劲制药有限责任公司 | Bone peptide beverage formula with blood replenishing effect and preparation method thereof |
| CN112494633A (en) * | 2020-11-30 | 2021-03-16 | 湖南天劲制药有限责任公司 | Bone peptide oral liquid production preparation system |
| CN112956583A (en) * | 2021-02-24 | 2021-06-15 | 北京金华雅轩健康科技有限公司 | Extraction process of fish collagen peptide |
| CN116590370A (en) * | 2023-04-24 | 2023-08-15 | 安徽盛美诺生物技术有限公司 | Cartilage peptide capable of promoting chondrocyte growth and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| TATEIWA D等: "Cartilage and Bone Destruction in Arthritis: Pathogenesis and Treatment Strategy: A Literature Review", CELLS, vol. 08, no. 08, 2 August 2019 (2019-08-02), pages 818 * |
| 周婷等: "鸡膝软骨胶原蛋白肽的超声辅助酶提工艺优化及抗氧化性", 肉类研究, vol. 35, no. 04, 30 April 2021 (2021-04-30), pages 7 - 15 * |
| 王瑞琦等: "鸡胸软骨糖/肽水解物的结构表征及其抗炎活性", 现代食品科技, vol. 37, no. 08, 21 May 2021 (2021-05-21), pages 44 - 52 * |
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