CN117618283B

CN117618283B - Oil-control anti-acne facial cleanser composition, application thereof and gel

Info

Publication number: CN117618283B
Application number: CN202311691564.XA
Authority: CN
Inventors: 丁文; 叶天赐; 凌沛学; 亓金亮; 王鹏
Original assignee: Shandong Meimei Pharmaceutical Co ltd; Meiye Biomedical Co ltd
Current assignee: Shandong Meimei Pharmaceutical Co ltd; Meiye Biomedical Co ltd
Priority date: 2023-12-11
Filing date: 2023-12-11
Publication date: 2024-06-14
Anticipated expiration: 2043-12-11
Also published as: CN117618283A

Abstract

The application belongs to the technical field of cosmetic production, and discloses an oil-control anti-acne facial cleanser composition which comprises the following components in percentage by mass of 0.01-5: 0.1 to 25: the oil-control anti-acne facial cleanser comprises 0.001-1 part of sodium hyaluronate HA60, PEG-20 triisostearate, butyl avocado oleate and 69-99.889 parts of gel carrier, wherein the oil-control anti-acne facial cleanser can deeply clean skin grease and is favorable for inhibiting sebum secretion so as to achieve the effects of controlling oil and resisting acne.

Description

Oil-control anti-acne facial cleanser composition, application thereof and gel

Technical Field

The invention relates to the technical field of cosmetic production, in particular to an oil-control anti-acne facial cleanser composition, application thereof and gel.

Background

The grease on the skin surface is mainly sebum secreted by sebaceous glands, and the secreted sebum reaches the skin surface along a follicular duct to finish the seborrhea. However, due to the special structure of the follicular orifice, follicular orifice corner plugs are easily formed by hyperkeratosis, normal sebum discharge is blocked, micro-acne is formed, meanwhile, acne shortrod bacterins are greatly proliferated, and visible acne-like symptoms such as nodules are formed by further development. The role of sebaceous glands in secreting sebum is mainly controlled by hormonal stimulation, with 5 a-reductase being one of the key hormones for acne production. Therefore, the preparation can inhibit hormone to stimulate sebaceous glands to generate sebum, dissolve follicular micro-acne, and make deep skin clean, thereby being beneficial to relieving acne and acnes. The effective treatment measures of the skin beauty center for acne are to remove the acne content in hair follicles by various modes, and finally to disinfect, anti-inflammatory, care and the like again so as to restore the skin to a healthy state.

The Chinese patent 202210982728.3 discloses a mild low-sensitivity moisturizing acne-removing gel and a preparation method thereof, wherein the gel comprises the following components in percentage by weight: 0.5-2% of salicylic acid and 0.5-6% of polyhydroxycarboxylic acid, wherein the solubilizer is selected from poloxamer.

In the mild and low-sensitivity moisturizing acne-removing gel prepared by the scheme, salicylic acid shallow layers are stripped, and excessive oil is prevented and treated, and closed mouth and acne (blackhead and whitehead) are treated; azelaic acid is anti-inflammatory and antibacterial, and is used for preventing and treating inflammatory red swelling type acnes and acne marks; the polyhydroxycarboxylic acid promotes biosynthesis of glycosaminoglycans or collagen in the skin, and plays a role in moisturizing, relieving and repairing. The proposal utilizes the specific proportion of the three components to make the effects complementary, synergize and moisten the skin mildly and lowly, and simultaneously has high-efficiency and comprehensive prevention and treatment effects on different types of acnes, and has excellent acne-removing effect.

Chinese patent 202010455320.1 discloses an oil-control acne-removing composition which comprises the following components: 5-10 parts of lactobacillus/pear juice fermentation product filtrate, 2-4 parts of white willow bark extract, 5-14 parts of cortex phellodendri extract, 5-12 parts of cordate houttuynia extract, 3-10 parts of Japanese beautyberry extract, 1-6 parts of matrine, 3-14 parts of centella asiatica extract, 2-6 parts of radix scutellariae extract, 0.1-4 parts of royal jelly oil control composition, 0.5-2 parts of olive leaf extract, 2-6 parts of peony root extract, 1-2 parts of mountain branchlet and 0.5-3 parts of bergamot leaf extract; the royal jelly oil control composition comprises the following components in percentage by weight of 80-82:4-8: 4-6:4-8 of 1, 3-butanediol, 10-hydroxydecanoic acid, sebacic acid and 1, 10-decanediol.

According to the scheme, lactobacillus/pear juice fermentation product filtrate with pore dredging effect and white willow bark extract, cortex phellodendri extract with anti-inflammatory effect, herba houttuyniae extract with anti-inflammatory effect, japanese beautyberry extract with skin antioxidation and sebum oxidation prevention function, matrine with antimicrobial activity, centella asiatica extract for relieving skin sensitivity, elastase-inhibiting, free radical-eliminating and antioxidation baical skullcap root extract, a royal jelly oil control composition for reestablishing sebum balance of acne-prone skin, an olive leaf extract with ultraviolet-proof effect, a peony root extract with inhibition effect on metalloprotease and elastase, a mountain-cloth dead leaf extract capable of controlling grease secretion frequency, and bergamot leaf extract with moisturizing and antioxidation effect are adopted, and the oil control acne removal composition realizes anti-acne maintenance stability and effective prevention of vaccinia repetition through compounding of the substances; at the same time, it can be seen that this solution does not achieve the anti-acne objective from the standpoint of inhibition of 5α -reductase.

Chinese patent 202110940452.8 discloses an oil control composition containing a plant active ingredient comprising a component that inhibits 5α -reductase, wherein the component employed that inhibits 5α -reductase comprises a bioflavonoid, preferably a plant acanthus (Maclura cochinchinensis) leaf extract.

According to the scheme, bioflavonoids, dog rose hip extracts and sarcosine are combined to form an oil control scheme, sebum secretion is regulated from two targets of inhibiting 5 a-reductase and sebaceous gland differentiation, meanwhile, skin is relieved, skin pressure is reduced, and the activity of sebaceous cells is reduced, so that the aim of controlling oil more efficiently is achieved.

The problem that this scheme needs to solve: a novel oil-controlling anti-acne composition capable of reducing excessive sebum secretion is developed.

Disclosure of Invention

The invention aims to provide an oil-control anti-acne cleansing composition, which has the capabilities of water locking, moisture preservation, oxidation resistance, inflammation resistance, oil control and the like, and the oil-control anti-acne cleansing gel can inhibit the expression of 5 a-reductase, further reduce sebum secretion, effectively deeply clean sebum and other secretions in facial hair follicles, and reduce hair follicle blockage.

In order to achieve the aim, the application discloses an oil-control anti-acne facial cleanser composition which comprises, by mass, sodium hyaluronate HA60, PEG-20 triisostearate and butyl avocado oleate, wherein the mass ratio of the sodium hyaluronate HA60 to the PEG-20 triisostearate to the butyl avocado oleate is 0.01-5: 0.1 to 25:0.001 to 1.

Preferably, the mass ratio of the sodium hyaluronate HA60, the PEG-20 glycerol triisostearate and the butyl avocado oleate is 0.01-2: 0.1 to 5:0.001 to 0.2.

In addition, the application also discloses application of the oil-control anti-acne facial cleanser composition as an oil-control anti-acne facial cleanser active ingredient in daily chemicals.

In addition, the application also discloses an oil-control anti-acne facial cleanser, which comprises, by mass, 0.111-31 parts of the oil-control anti-acne facial cleanser and 69-99.889 parts of a gel carrier.

Preferably, the gel carrier comprises the following components in parts by mass:

the beneficial effects of the application are as follows:

The sodium hyaluronate can form a layer of film on the surface of skin to lock moisture, so that the skin is kept moist, moisture loss is prevented, and the elasticity and luster of the skin are maintained; meanwhile, the sodium hyaluronate has good moisturizing and repairing effects on skin, can promote the growth and repairing of cells, improve the rough and dry skin and other problems, enhance the nutrition absorption capacity of skin cells, promote the generation of collagen and elastic fibers, improve the elasticity and compactness of the skin, reduce wrinkles and fine lines and effectively resist aging. The sodium hyaluronate can also absorb ultraviolet rays to prevent the skin from being damaged by the ultraviolet rays, and meanwhile, the sodium hyaluronate can influence the transparency of the stratum corneum to increase the luster and the transparency of the skin;

The PEG-20 triisostearate can form a protective film, prevent water loss, keep skin moist, and meanwhile, the PEG-20 triisostearate is an excellent emulsifier, and can uniformly mix water and oil, so that the cosmetic is more stable. And the PEG-20 glycerol triisostearate has cleaning and makeup removing effects and can be used in a face cleaning product or a makeup removing product. PEG-20 triisostearate can also improve the luster and transparency of the skin, and make the skin more attractive and attractive. PEG-20 triisostearate can relieve skin irritation of cosmetics and reduce skin allergy occurrence probability;

The butyl avocado oleate provided by the application can be quickly absorbed by skin, a protective layer is formed on the skin, moisture is locked, and the moisture retention capacity of the skin is enhanced, so that the skin is moistened and soft. Meanwhile, the avocado butyl oleate contains abundant vitamin E and various antioxidant substances, can help skin resist free radical attack, reduces oxidative damage, and is beneficial to skin health and luster. The avocado butyl oleate can promote the growth and repair of skin cells, improve the problems of skin texture, darkness and the like, and has good relieving effects on the problems of skin inflammation, sensitivity and the like. The avocado butyl oleate can also permeate into deep layers of skin, regulate skin grease balance and shrink pores, thereby improving skin texture and enabling skin to be smoother and finer. In addition, the avocado butyl oleate is a stable grease component, has a longer shelf life and can prolong the service life of cosmetics.

The application realizes the effective inhibition of the expression of 5 a-reductase by compounding the substances and using the substances together with gel, thereby reducing sebum secretion, and the oil-controlling anti-acne cleansing gel can effectively clean sebum and other secretions in the hair follicles of the face deeply and reduce the blockage of the hair follicles by the synergistic effect of the substances.

Detailed Description

The present invention will be described more fully hereinafter with reference to the accompanying drawings, in which specific conditions, either conventional or manufacturer-suggested, are not explicitly stated. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.

Before describing the examples in detail, the following description is made of the information of the raw materials used in the examples:

sodium hyaluronate HA60: purchased from biotechnology limited company under the east of shan through the sky;

PEG-20 glycerol triisostearate: purchased from irinotecan;

butyl avocado oleate: purchased from Laboratoires Expanscience;

Potassium cocoyl glycinate: purchased from Changsha Puji Biotech Co., ltd;

glycerol: purchased from taco brown chemical (Zhangjihong Kong) Co., ltd;

sodium cocoyl methyl taurate: zhengzhou ren Ming chemical products Co., ltd;

Sodium cocoamidopropionate: gano chemical industry Co., ltd;

Sodium PCA: nanjing Baimuda Biotechnology Co., ltd;

Trehalose: zhejiang Tianqi bioengineering Co., ltd;

p-hydroxyacetophenone: shaanxi Saen Biotech Co., ltd;

Sodium lauroyl oat amino acid: shanxi Shangcheng Biotech Co., ltd;

maltooligosaccharide glucoside: shaanxi, shengzhi Biotech Co., ltd;

Xylitol-based glucoside: peptide loving biotechnology (western) limited;

acrylic/stearyl polyether-20 methacrylate crosslinked polymer: beijing Huamei reciprocal biochemical engineering, model Carbop Smart C-F;

potassium hydroxide: henan He Yu Jia chemical products Co., ltd;

1, 2-hexanediol: gano chemical industry Co., ltd;

examples 1 to 6

Preparation of gel carrier:

step 1: firstly, mixing 8 parts of potassium cocoyl glycinate, 5 parts of glycerol, 2 parts of sodium cocoyl methyl taurate, 1.5 parts of sodium cocoyl amino propionate, 1.5 parts of PCA sodium, 0.75 part of trehalose, 0.6 part of p-hydroxyacetophenone, 0.2 part of lauroyl oat amino acid sodium, 0.2 part of maltooligosaccharide glucoside and 0.15 part of xylitol-based glucoside with part of water, heating, stirring and dissolving for later use;

step 2: mixing part of water with 2 parts of acrylic acid (ester) or stearyl alcohol polyether-20 methacrylate cross-linked polymer uniformly, adding into the solution in the step 1, and stirring uniformly;

step 3: mixing part of water with 0.5 part of potassium hydroxide uniformly, adding into the solution, and stirring uniformly;

step 4: adding 0.6 part of1, 2-hexanediol into the solution, and fully and uniformly stirring.

The formula of the oil-control anti-acne facial cleanser composition and the gel are shown in table 1, and the formula is that only the mass parts of water in a gel carrier are changed:

TABLE 1

Example 7

Substantially the same as in example 1, except that the gel carrier was prepared by:

Step 1: firstly, mixing 7 parts of potassium cocoyl glycinate, 4 parts of glycerol, 1 part of sodium cocoyl methyl taurate, 1 part of sodium cocoyl aminopropionate, 1 part of PCA sodium, 0.5 part of trehalose, 0.1 part of p-hydroxyacetophenone, 0.1 part of lauroyl oat amino acid sodium, 0.1 part of maltooligosaccharide glucoside and 0.1 part of xylitol-based glucoside with part of water, heating, stirring and dissolving for later use;

Step 2: mixing part of water with 1 part of acrylic acid (ester) or stearyl alcohol polyether-20 methacrylate cross-linked polymer uniformly, adding into the solution in the step 1, and stirring uniformly;

Step 3: mixing part of water with 0.1 part of potassium hydroxide uniformly, adding into the solution, and stirring uniformly;

step 4: adding 0.4 part of1, 2-hexanediol into the solution, and fully and uniformly stirring.

Example 8

Step 1: firstly, mixing 9 parts of potassium cocoyl glycinate, 6 parts of glycerol, 3 parts of sodium cocoyl methyl taurate, 2 parts of sodium cocoyl aminopropionate, 2 parts of PCA sodium, 1 part of trehalose, 1 part of p-hydroxyacetophenone, 1 part of sodium lauroyl oat amino acid, 1 part of maltooligosaccharide glucoside and 1 part of xylitol-based glucoside with part of water, heating, stirring and dissolving for later use;

Step 2: mixing part of water with 3 parts of acrylic acid (ester) or stearyl alcohol polyether-20 methacrylate cross-linked polymer uniformly, adding into the solution in the step 1, and stirring uniformly;

Step 3: mixing part of water with 0.8 part of potassium hydroxide uniformly, adding into the solution, and stirring uniformly;

Step 4: adding 1 part of 1, 2-hexanediol into the solution, and fully and uniformly stirring.

Comparative examples 1 to 3

A composition gel having a formulation as shown in table 2:

The formulation is shown in Table 2:

TABLE 2

Comparative example 4

Substantially the same as in example 4, except that sodium hyaluronate was replaced with the same parts by weight of sodium polyglutamate.

Comparative example 5

Substantially the same as in example 4, except that the same parts by weight of PEG-6 caprylic/capric glyceride was used instead of PEG20 triisostearate.

Comparative example 6

Substantially the same as in example 4, except that the same mass fraction of butyl oleate was used instead of butyl avocado oleate.

Performance test:

irritation evaluation:

The gel prepared in examples 1 to 8 was taken as a sample, and skin irritation test for skin irritation/corrosiveness test showed that the gel prepared in examples 1 to 8 had no irritation to skin. And the invention has no photosensitive reaction through skin phototoxicity test.

Spot-mapping experiments:

The gel prepared in examples 1-8 was taken as a sample, and the results of the skin patch test on human bodies showed that no adverse reaction occurred.

Sample inhibition intracellular lipid synthesis assay:

lipid synthesis is regulated primarily by the endocrine system, while androgens (testosterone and DHT) are the primary factors affecting sebum secretion, which regulate sebaceous gland differentiation, proliferation and sebum synthesis and secretion; the 5 alpha-reductase can promote the generation of DHT, so as to promote the synthesis of lipid;

Brief description of the method: the project mainly takes SZ95 sebaceous gland cells as a research object, and selects proper induction conditions and proper male standard substances to evaluate the inhibition effect of the invention on the sebum secretion of the cells so as to judge the oil control effect.

Verification of the ability of the samples to inhibit intracellular lipid synthesis: SZ95 sebaceous gland cells were inoculated into 6-well cell culture plates, cultured for 48h at 2x10 ⁵ per well, and medium containing the gel prepared in example 2 was added, and the concentration was determined to be 0.01%,0.04%,0.08% according to the maximum drug concentration at which the gel had no significant effect on cell proliferation.

Meanwhile, berberine hydrochloride is used for replacing gel to prepare a culture medium as a positive control group, and the concentration of the berberine hydrochloride is 0.08%;

deionized water is used for replacing berberine hydrochloride to serve as a blank control group;

After further culturing for 48h, digestion was terminated with 0.25% trypsin containing 0.02% EDTA, and the digestion was stopped with 10% calf serum in medium, washed 2 times with PBS, single cell suspensions (in PBS) were prepared, nile red fluorescent dye was added at a final concentration of 10ng/mL, incubated at room temperature for 15min, filtered with a 300 mesh nylon filter, 10000 cells in each sample were detected on a flow cytometer, the average fluorescence intensity of each cell was calculated, and 3 parallel wells were set for each concentration sample.

Discussion of results: compared with the control group, the three concentration samples and the positive control have the effect on the lipid synthesis in SZ95 cells, wherein the effect is reduced by 2.7% in the 0.01% group, 18.1% in the 0.04% group and 30.3% in the 0.08% group; the positive control group decreased by 41.7%, which indicates that the sample group can effectively inhibit the synthesis of lipid in SZ95 sebaceous gland cells.

Comparison of the ability to inhibit intracellular lipid synthesis between examples 1-6: SZ95 (human sebaceous gland cells) cells were inoculated into 6-well cell culture plates, each well was incubated for 48 hours at 2x10 ⁵, and the concentrations of the gels in the above culture media were 0.08% each, respectively, containing the gel prepared in example 1, the gel prepared in example 2, the gel prepared in example 3, the gel prepared in example 4, the gel prepared in example 5, and the gel prepared in example 6.

After further culturing for 48h, digestion was terminated with 0.25% trypsin containing 0.02% EDTA, and the digestion was stopped with 10% calf serum in medium, washed 2 times with PBS, single cell suspensions (in PBS) were prepared, nile red fluorescent dye was added at a final concentration of 10ng/mL, incubated at room temperature for 15min, filtered with a 300 mesh nylon filter, 10000 cells in each sample were detected on a flow cytometer, the average fluorescence intensity of each cell was calculated, and 3 parallel wells were provided for each sample.

Discussion of results: examples 1-6 were compared to each other for effects on lipid synthesis in SZ95 cells, 19.4% decrease in example 1, 32.5% decrease in example 2, 18.1% decrease in example 3, 19.9% decrease in example 4, 26.3% decrease in example 5, and 18.7% decrease in example 6. From the results, it can be demonstrated that the gel of example 2 has the most remarkable effect of inhibiting the synthesis of lipid in SZ95 sebaceous gland cells.

Comparison of the ability of example 2 to comparative examples 1-3 to inhibit intracellular lipid synthesis: SZ95 (human sebaceous gland cells) cells were inoculated into 6-well cell culture plates, each well was incubated for 48 hours at 2x10 ⁵, and the gel prepared in comparative example 1, comparative example 2, comparative example 3, and example 2 were added, respectively, at a concentration of 0.08% in the above medium. After further culturing for 48h, digestion was terminated with 0.25% trypsin containing 0.02% EDTA, and the digestion was stopped with 10% calf serum in medium, washed 2 times with PBS, single cell suspensions (in PBS) were prepared, nile red fluorescent dye was added at a final concentration of 10ng/mL, incubated at room temperature for 15min, filtered with a 300 mesh nylon filter, 10000 cells in each sample were detected on a flow cytometer, the average fluorescence intensity of each cell was calculated, and 3 parallel wells were provided for each sample.

Discussion of results: comparative examples 1-3 have 11.7% lower effect on SZ95 intracellular lipid synthesis compared to example 2, 13.9% lower in comparative example 1, and 10.3% lower in comparative example 3; whereas example 2 decreased by 29.1%, it was demonstrated that the gel inhibition effect of lipid synthesis in SZ95 sebaceous gland cells was decreased in the absence of any of sodium hyaluronate HA60, PEG-20 triisostearate and butyl avocado oleate.

Comparison of the ability of example 2 to comparative examples 4-6 to inhibit intracellular lipid synthesis: SZ95 (human sebaceous gland cells) cells were inoculated into 6-well cell culture plates, each well was incubated for 48 hours at 2x10 ⁵, and the gel prepared in comparative example 4, comparative example 5, comparative example 6, and example 2 were added, respectively, at a concentration of 0.08% in the above medium.

Discussion of results: comparative examples 4-6 have 12.5% lower effect on SZ95 intracellular lipid synthesis compared to example 2, 11.2% lower in comparative example 4, 11.1% lower in comparative example 5, and 11.1% lower in comparative example 6; while the decrease in the group of example 2 was 31.5%, it was revealed that the inhibition of the synthesis of lipid in SZ95 sebaceous gland cells by the gel was decreased by replacing any of the above components with components close to sodium hyaluronate HA60, PEG-20 glycerol triisostearate, and butyl avocado oleate.

From the results of the performance test, the inhibition capability of the gel on intracellular lipid synthesis is obviously reduced after the gel prepared by compounding sodium hyaluronate HA60, PEG-20 glycerol triisostearate and avocado butyl oleate is lack or any component is replaced by similar substances.

Oil control efficacy human body evaluation:

The safety evaluation of the sample before the experiment passes through cosmetic safety technical Specification (2022 edition) to confirm that the sample is safe and has no side reaction.

The experiment was divided into a positive control group, a blank group and a sample group, wherein the average grease secretion amount of volunteers changes with time after using the product, the sample group uses the gel prepared in example 2, the control group uses an equivalent amount of berberine hydrochloride solution with concentration of 0.2%, and the blank group uses an equivalent amount of deionized water.

Washing off with clear water after 15min, wherein the sebum secretion of the volunteers in the control group is reduced by 30.11% compared with that in the blank group, and the sebum secretion of the volunteers in the sample group is reduced by 25.32% compared with that in the blank group; after 6 hours, the control group and the sample group were reduced by 14.97% and 12.36% respectively from the blank group, and it was found that the control group and the sample group were significantly different from the blank group. Therefore, the sample group can effectively control oil and has anti-acne effect.

Claims

1. The oil-control anti-acne cleansing gel is characterized by comprising, by mass, 0.111-31 parts of oil-control anti-acne cleansing composition and 69-99.889 parts of gel carrier;

The oil-control anti-acne facial cleanser composition comprises sodium hyaluronate HA60, PEG-20 glycerol triisostearate and butyl avocado oleate, wherein the mass ratio of the sodium hyaluronate HA60 to the PEG-20 glycerol triisostearate to the butyl avocado oleate is 0.01-5: 0.1 to 25:0.001 to 1;

The gel carrier comprises the following components in parts by mass:

5-10 parts of potassium cocoyl glycinate;

2-8 parts of glycerol;

1-3 parts of acrylic acid (ester)/stearyl alcohol polyether-20 methacrylate cross-linked polymer;

1-3 parts of cocoyl methyl taurine sodium;

1-3 parts of sodium cocoamidopropionate;

1-3 parts of PCA sodium;

0.5-1 part of trehalose;

0.1 to 1 part of potassium hydroxide;

0.1-1 part of 1, 2-hexanediol;

0.1 to 1 part of p-hydroxyacetophenone;

0.1 to 1 part of lauroyl oat amino acid sodium;

0.1-1 part of malto-oligosaccharide glucoside;

0.1 to 1 part of xylitol-based glucoside;

the water is supplemented to 69-99.889 parts.

2. The oil-control anti-acne cleansing gel according to claim 1, wherein the gel carrier comprises the following components in parts by mass:

7-9 parts of potassium cocoyl glycinate;

4-6 parts of glycerol;

1-3 parts of cocoyl methyl taurine sodium;

1-2 parts of sodium cocoamidopropionate;

1-2 parts of PCA sodium;