CN117551537B - Bacteria detection sampling device for hemodialysis machine - Google Patents

Bacteria detection sampling device for hemodialysis machine Download PDF

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Publication number
CN117551537B
CN117551537B CN202410038858.0A CN202410038858A CN117551537B CN 117551537 B CN117551537 B CN 117551537B CN 202410038858 A CN202410038858 A CN 202410038858A CN 117551537 B CN117551537 B CN 117551537B
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centrifugal
plate
cylinder
collecting
shell
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CN117551537A (en
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高翔
徐德超
吴建祥
卞蓉蓉
钱一欣
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Second Affiliated Hospital Army Medical University
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Second Affiliated Hospital Army Medical University
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/10Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Centrifugal Separators (AREA)

Abstract

The invention relates to the field of hemodialysis detection, in particular to a bacteria detection sampling device for a hemodialysis machine, which comprises a shell, wherein a collection mechanism is arranged in the shell and is used for collecting bacteria; the collecting mechanism is provided with an adjusting mechanism which is used for isolating collected bacteria from the outside; a centrifugal mechanism is arranged in the shell and is used for centrifuging collected bacteria; the fluorescent instrument is arranged at the top of the shell, a centrifugal mechanism is arranged below the fluorescent instrument, a driving mechanism for driving the centrifugal mechanism to rotate is arranged inside the shell, a waste bucket is arranged below the centrifugal mechanism, and the waste bucket is arranged inside the shell; the inside of shell is provided with the sterilamp, the sterilamp is used for killing the bacterium on centrifugal mechanism, the collection mechanism.

Description

Bacteria detection sampling device for hemodialysis machine
Technical Field
The invention relates to the field of hemodialysis detection, in particular to a bacteria detection sampling device for a hemodialysis machine.
Background
Hemodialysis is mainly to filter blood through an external device so as to discharge impurities in the blood. The blood needs to be detected at the water inlet and the bypass of the dialysis machine before dialysis, so that the dialysis machine can ensure that a patient subjected to dialysis can reduce bacterial infection during dialysis. The existing sampling device is required to sample, and then cannot detect in time, the sampled sample is required to be sent to a corresponding detection place in the middle for detection, detection time is prolonged, the middle part drives the sampling device to move and is exposed in the air, other bacteria are easy to be stained, detection results are not accurate enough, A liquid and B liquid of a connecting pipe of an external dialysis machine are easy to be polluted, and risks of infection of other diseases of patients in the dialysis process are increased.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a bacteria detection sampling device for a hemodialysis machine.
The technical scheme adopted for solving the technical problems is as follows: the bacteria detection sampling device for the hemodialysis machine comprises a shell, wherein an acquisition mechanism is arranged in the shell and is used for acquiring bacteria; the collecting mechanism is provided with an adjusting mechanism which is used for isolating collected bacteria from the outside; a centrifugal mechanism is arranged in the shell and is used for centrifuging collected bacteria; the fluorescent instrument is arranged at the top of the shell, a centrifugal mechanism is arranged below the fluorescent instrument, a driving mechanism for driving the centrifugal mechanism to rotate is arranged inside the shell, a waste bucket is arranged below the centrifugal mechanism, and the waste bucket is arranged inside the shell; the inside of shell is provided with the sterilamp, the sterilamp is used for killing the bacterium on centrifugal mechanism, the collection mechanism.
Preferably, the collecting mechanism comprises a collecting cylinder, a collecting cavity is arranged at the end part of the collecting cylinder, and a cotton swab for adsorbing bacteria is placed in the collecting cavity; the inside of gathering the section of thick bamboo is provided with and is located gather the otter board of chamber top, the inside of gathering the section of thick bamboo is provided with the board groove, the top of board groove is provided with the upset board, the inside of gathering the section of thick bamboo is provided with the stabilizer bar, rotate on the stabilizer bar and be connected with the upset board.
Preferably, the collecting mechanism further comprises a push rod arranged on the collecting cylinder in a sliding manner, a piston positioned in the collecting cylinder is arranged at the end part of the push rod, an inner cavity is formed in the push rod, and the inner cavity is used for storing purified water; two first one-way valves are arranged on the rod wall of the push rod, and two second one-way valves are arranged on the inner wall of the collection cylinder; when the push rod moves towards the outside of the collection cylinder, the second one-way valve is used for water inflow, and when the push rod moves towards the inside of the collection cylinder, the first one-way valve is used for water inflow.
Preferably, the collecting mechanism further comprises a sliding block arranged on the outer wall of the collecting cylinder, and external threads are arranged on the outer portion of the end portion of the collecting cylinder.
Preferably, the adjusting mechanism comprises a rotary drum which is arranged outside the collecting cylinder and is in spiral connection with the collecting cylinder, and the rotary drum is in rotary connection with the sleeve through a support; the bottom end of the sleeve is provided with a stabilizing plate, and the stabilizing plate is fixed with the sleeve through a plurality of fixing rods; an inner cylinder is rotatably arranged in the sleeve, and the inner cylinder synchronously rotates along with the rotation of the rotary cylinder; the middle part of stabilizer board is provided with the sealed hole, be provided with on the stabilizer board and be used for carrying out the shutoff to the sealed hole and open and close the subassembly, be provided with the promotion subassembly on the stabilizer board, the promotion subassembly is used for the opening and shutting of drive opening and close the subassembly.
Preferably, the pushing component comprises a rotating rod arranged on the inner wall of the inner cylinder, a rotating rod is rotatably arranged on the stabilizing plate, and a plurality of stop rods matched with the rotating rod are arranged on the outer wall of the rotating rod; the outer surface of the rotating rod is provided with a second clamping tooth, and the second clamping tooth is meshed with the opening and closing assembly.
Preferably, the opening and closing assembly comprises a driven ring meshed with the second latch, a guide groove is formed in the driven ring, a guide column is arranged in the guide groove, the guide column is fixed on the stabilizing plate, and the driven ring rotates on the stabilizing plate; the automatic sealing device is characterized in that a pull rod is rotatably arranged on the driven ring, the end part of the pull rod is rotatably connected with a sealing plate, the sealing plate is rotatably arranged on the stabilizing plate through a rotary pin, and the sealing plate is used for sealing the sealing hole.
Preferably, the centrifugal mechanism comprises a centrifugal cylinder rotatably arranged in the shell, a rotary table is arranged on the centrifugal cylinder, a bearing is arranged on the rotary table, and the bearing is rotatably arranged in the shell; a plurality of unidirectional rotating ratchets are arranged on the outer wall of the turntable; a plurality of placing grooves are formed in the centrifugal cylinder and used for placing cotton swabs after bacteria are collected; a centrifugal plate in spiral connection with the centrifugal cylinder is arranged below the centrifugal cylinder, a blanking port is arranged in the middle of the centrifugal plate, and a waste material cylinder is arranged right below the blanking port; a plurality of first latches are arranged on the outer wall of the centrifugal plate.
Preferably, the driving mechanism comprises a driving wheel meshed with the first latch, a motor is arranged in the shell, the driving wheel is arranged on an output shaft of the motor, and when the motor rotates, the centrifugal plate is driven to drive the centrifugal cylinder to synchronously rotate.
The beneficial effects are that: the collecting mechanism is arranged to directly collect the pipeline interface of the hemodialysis machine and store the collected sample, and the collected bacteria can be separated from the outside through the adjusting mechanism, so that the sample can be placed to be polluted when the sample is stored; the collected sample can be subjected to centrifugal treatment through the centrifugal mechanism, so that bacteria can be detected through a fluorometer in the later period; bacteria after detection can be killed through the sterilizing lamp, so that bacteria residues and detection results cannot be influenced when the collecting mechanism and the centrifugal mechanism are used next time; can collect the bacterium after killing through the waste bin that sets up, handle through taking out the waste bin from the inside of shell after collecting.
Drawings
The invention will be further described with reference to the drawings and examples.
FIG. 1 is a schematic diagram of the overall structure of the present invention;
FIG. 2 is a schematic view of the position structure of the centrifugal mechanism of the present invention;
FIG. 3 is a schematic view of the internal structure of the present invention;
FIG. 4 is a schematic view of the centrifugal mechanism of the present invention;
FIG. 5 is a schematic diagram of the connection of the adjustment mechanism and the collection mechanism of the present invention;
FIG. 6 is an enlarged schematic view of the structure of FIG. 5 at A;
Fig. 7 is a schematic view of the internal structure of the collecting mechanism of the present invention. In the figure: 1. a housing; 11. a mounting plate; 12. a sterilization tank; 13. a clamping plate; 2. a fluorometer; 4. a centrifugal mechanism; 41. a centrifugal barrel; 42. a turntable; 43. a ratchet; 44. a bearing; 45. a placement groove; 46. a feed opening; 47. a centrifugal plate; 48. a first latch; 5. an adjusting mechanism; 51. a sleeve; 52. a rotating drum; 53. an inner cylinder; 54. a stabilizing plate; 55. a pushing assembly; 551. a rotating rod; 552. a second latch; 553. a stop lever; 554. a rotating lever; 56. a fixed rod; 57. an opening and closing assembly; 571. a slave ring; 572. a guide groove; 573. a guide post; 574. a pull rod; 575. a plugging plate; 576. a rotary pin; 58. sealing the hole; 6. a collection mechanism; 61. a collection cylinder; 62. a slide block; 63. an external thread; 64. a push rod; 65. a top cover; 66. an inner cavity; 67. a first one-way valve; 68. a second one-way valve; 69. a turnover plate; 600. a piston; 610. a stabilizer bar; 611. a plate groove; 612. a screen plate; 613. a collection chamber; 7. a driving mechanism; 71. a driving wheel; 72. a motor; 8. a waste bin; 9. and a sterilizing lamp.
Detailed Description
The invention is further described in connection with the following detailed description in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the invention easy to understand.
In one embodiment, referring to fig. 1-7 of the specification, the bacteria detection sampling device for a hemodialysis machine of the present invention includes a housing 1, a collection mechanism 6 is installed in the housing 1, and the collection mechanism 6 is used for collecting bacteria; an adjusting mechanism 5 is arranged on the collecting mechanism 6, and the adjusting mechanism 5 is used for isolating collected bacteria from the outside; a centrifugal mechanism 4 is arranged in the shell 1, and the centrifugal mechanism 4 is used for centrifuging collected bacteria; the fluorescent lamp is characterized in that a fluorescent instrument 2 is arranged at the top of the shell 1, a centrifugal mechanism 4 is arranged below the fluorescent instrument 2, a driving mechanism for driving the centrifugal mechanism 4 to rotate is arranged inside the shell 1, a waste bin 8 is arranged below the centrifugal mechanism 4, and the waste bin 8 is arranged inside the shell 1; the inside of shell 1 is provided with sterilamp 9, sterilamp 9 is used for killing the bacterium on centrifugal mechanism 4, the collection mechanism 6.
The sampling device can directly collect the pipeline interface of the hemodialysis machine through the collection mechanism 6, store the collected sample, and separate the collected bacteria from the outside through the regulating mechanism 5, so that the sample can be placed to be polluted when the sample is stored; the collected sample can be subjected to centrifugal treatment through the centrifugal mechanism 4, so that bacteria can be detected through the fluorometer 2 in the later period; bacteria after detection can be killed through the sterilizing lamp 9, so that bacteria residues are avoided when the collecting mechanism 6 and the centrifugal mechanism 4 are used next time, and the detection result is not affected; bacteria after killing can be collected through the waste barrel 8, and after the bacteria are well collected, the bacteria are taken out from the shell 1 through the waste barrel 8 for treatment.
The collecting mechanism 6 comprises a collecting barrel 61, a collecting cavity 613 is arranged at the end part of the collecting barrel 61, and a cotton swab for adsorbing bacteria is placed in the collecting cavity 613; the inside of collection section of thick bamboo 61 is provided with the otter board 612 that is located collection chamber 613 top, the inside of collection section of thick bamboo 61 is provided with board groove 611, the top of board groove 611 is provided with the upset board 69, the inside of collection section of thick bamboo 61 is provided with the stabilizer bar 610, rotate on the stabilizer bar 610 and be connected with upset board 69.
The collecting mechanism 6 further comprises a push rod 64 arranged on the collecting barrel 61 in a sliding manner, a piston 600 positioned in the collecting barrel 61 is arranged at the end part of the push rod 64, an inner cavity 66 is arranged in the push rod 64, and the inner cavity 66 is used for storing purified water; the rod wall of the push rod 64 is provided with two first one-way valves 67, and the inner wall of the collection cylinder 61 is provided with two second one-way valves 68; the second check valve 68 is filled with water when the push rod 64 moves toward the outside of the collection tube 61, and the first check valve 67 is filled with water when the push rod 64 moves toward the inside of the collection tube 61.
The collection mechanism 6 further comprises a sliding block 62 arranged on the outer wall of the collection barrel 61, and an external thread 63 is arranged on the outer part of the end part of the collection barrel 61.
By adding a cotton swab inside collection chamber 613, so that cotton swab blocks collection chamber 613, cotton swab cannot enter plate groove 611 due to otter board 612, otter board 612 can also provide enough flow space for water flow during flushing; when the cotton swab is installed, a small part of cotton swab heads need to be left outside the collecting cavity 613; the end part of the cotton swab stretches into the collecting pipeline, and after the collection is completed, the adjusting mechanism 5 is rotated to enable the cotton swab to be accommodated in the adjusting mechanism 5; when the cotton swab is required to be placed in the centrifugal mechanism 4, the cotton swab extends out of the regulating mechanism 5 by rotating the regulating mechanism 5, the push rod 64 is slowly extruded, and the cotton swab is slowly pulled outwards after extrusion, so that a certain amount of water is stored between the turnover plate 69 and the piston 600; when the push rod 64 is pushed again so that the push rod 64 moves towards the inside of the collecting barrel 61, the piston 600 at the end part of the push rod 64 moves synchronously, the piston 600 pushes purified water below to squeeze the turnover plate 69, the turnover plate 69 turns downwards, water flow can be impacted on a cotton swab through the screen 612 after the turnover plate 69 turns downwards along the plate groove 611, the cotton swab is separated from the inside of the collecting cavity 613, and the whole cotton swab can enter the inside of the centrifugal mechanism 4.
The adjusting mechanism 5 comprises a rotary drum 52 which is arranged outside the collecting drum 61 and is in spiral connection with the collecting drum 61, and the rotary drum 52 is in rotary connection with the sleeve 51 through a support; the bottom end of the sleeve 51 is provided with a stabilizing plate 54, and the stabilizing plate 54 is fixed with the sleeve 51 through a plurality of fixing rods 56; an inner cylinder 53 is rotatably arranged in the sleeve 51, and the inner cylinder 53 rotates synchronously along with the rotation of the rotary cylinder 52; the middle part of stabilizer plate 54 is provided with sealed hole 58, be provided with on the stabilizer plate 54 and be used for carrying out the shutoff to sealed hole 58 and open and close subassembly 57, be provided with on the stabilizer plate 54 and promote the subassembly 55, promote the subassembly 55 and be used for the opening and shutting of drive opening and close subassembly 57.
The pushing assembly 55 comprises a rotating rod 554 arranged on the inner wall of the inner cylinder 53, a rotating rod 551 is rotatably arranged on the stabilizing plate 54, and a plurality of stop rods 553 matched with the rotating rod 554 are arranged on the outer wall of the rotating rod 551; the outer surface of the rotating rod 551 is provided with a second latch 552, and the second latch 552 is meshed with the opening and closing component 57.
The opening and closing assembly 57 includes a driven ring 571 engaged with the second latch 552, a guide groove 572 is provided on the driven ring 571, a guide post 573 is provided inside the guide groove 572, the guide post 573 is fixed on the stabilizing plate 54, and the driven ring 571 rotates on the stabilizing plate 54; the pull rod 574 is rotatably arranged on the driven ring 571, a sealing plate 575 is rotatably connected to the end part of the pull rod 574, the sealing plate 575 is rotatably arranged on the stabilizing plate 54 through a rotating pin 576, and the sealing plate 575 is used for sealing the sealing hole 58.
When the position of the collecting barrel 61 in the sleeve 51 needs to be adjusted, the rotary barrel 52 is rotated, the rotary barrel 52 rotates to drive the external screw threads 63 arranged on the outer wall of the collecting barrel 61, and the sliding block 62 slides on the inner wall of the sleeve 51, so that the sliding block 62 plays a guiding role, and the rotary barrel 52 is forced to rotate to drive the collecting barrel 61 to linearly move and cannot rotate.
In order to improve the collection and protection efficiency, when the rotary drum 52 is rotated, the rotary drum 52 and the sleeve 51 are rotated relatively, so that the rotary drum 52 drives the inner drum 53 to rotate, the inner drum 53 rotates to drive the rotating rod 554 arranged on the inner wall of the inner drum to synchronously rotate, the rotating rod 554 rotates to abut against one side of the stop rod 553, and the rotating rod 554 drives the stop rod 553 to synchronously rotate until the rotating rod 554 rotates to a position separated from the stop rod 553 along with the inner drum 53; the rotating rod 554 continuously rotates along with the inner cylinder 53, when the inner cylinder 53 rotates for one circle, the rotating rod 554 pushes one stop lever 553 to rotate once, and when the stop lever 553 rotates, the stop lever 553 pushes the rotating rod 551 to rotate, and the rotating rod 551 rotates and then drives the second clamping tooth 552 to rotate; the second latch 552 rotates to drive the driven ring 571 to rotate, the driven ring 571 rotates to drive the pull rod 574 arranged on the inner part of the driven ring 571 to rotate, the end part of the pull rod 574 is connected with the sealing plate 575, and when the pull rod 574 pulls the sealing plate 575, the sealing plate 575 rotates around the rotating pin 576; until the plurality of closure plates 575 exit from the sealing aperture 58, at which point the cotton bud extends just outside the sleeve 51 through the sealing aperture 58; at this time, the cotton swab is put into the inside of the centrifugal mechanism 4, and is flushed into the inside of the centrifugal mechanism 4 by pushing the push rod 64.
The centrifugal mechanism 4 comprises a centrifugal cylinder 41 rotatably arranged in the shell 1, a rotary table 42 is arranged on the centrifugal cylinder 41, a bearing 44 is arranged on the rotary table 42, and the bearing 44 is rotatably arranged in the shell 1; a plurality of ratchet teeth 43 which rotate unidirectionally are arranged on the outer wall of the rotary table 42; a plurality of placing grooves 45 are formed in the centrifugal barrel 41, and the placing grooves 45 are used for placing cotton swabs after bacteria are collected; a centrifugal plate 47 in spiral connection with the centrifugal cylinder 41 is arranged below the centrifugal cylinder 41, a feed opening 46 is arranged in the middle of the centrifugal plate 47, and a waste material cylinder 8 is arranged right below the feed opening 46; the outer wall of the centrifugal plate 47 is provided with a plurality of first latches 48.
The driving mechanism 7 comprises a driving wheel 71 meshed with the first latch 48, a motor 72 is arranged in the casing 1, the driving wheel 71 is arranged on an output shaft of the motor 72, and when the motor 72 rotates, the centrifugal plate 47 is driven to drive the centrifugal cylinder 41 to synchronously rotate.
When the centrifugation is needed, the motor 72 is driven to rotate to drive the driving wheel 71 to rotate, the driving wheel 71 is driven to rotate by the first latch 48, the first latch 48 drives the centrifugal plate 47 to rotate, at the moment, the centrifugal plate 47 and the centrifugal cylinder 41 are connected together through a screw, the centrifugal plate 47 drives the centrifugal cylinder 41 to synchronously rotate, and further, the cotton swab in the placing groove 45 is centrifuged, after the centrifugation is completed, the fluorescent instrument 2 detects the centrifuged cotton swab, and when bacteria are detected, the data are displayed on the display screen of the shell 1; medical staff can judge whether bacteria exceed the standard according to the data; when the detection is completed, bacteria are required to be killed, the bacteria are irradiated on the centrifugal cylinder 41 and the centrifugal plate 47 through the sterilizing lamp 9, and the collecting cylinder 61 placed in the shell 1 is killed, the ultraviolet sterilizing lamp 9 can be selected by the sterilizing lamp 9, or the high-temperature sterilizing lamp 9 is adopted, after the inactivation is completed, the driving motor 72 reversely rotates, and when the motor 72 reversely rotates, the centrifugal cylinder 41 cannot synchronously rotate along with the centrifugal plate 47 due to the action of the ratchet 43, at the moment, the centrifugal plate 47 can downwards rotate along the direction of the centrifugal cylinder 41, and after the centrifugal plate 47 downwards rotates for a certain distance, a cotton swab in the placing groove 45 can enter the waste barrel 8 from the discharging opening 46. The motor 72 is started again, the driving wheel 71 drives the centrifugal plate 47 to rotate, and the centrifugal plate 47 is pushed to the uppermost side due to the fact that the centrifugal plate 47 and the centrifugal cylinder 41 are connected in a spiral mode, and the centrifugal cylinder 41 is driven to synchronously rotate by continuing to rotate, at the moment, the motor 72 is stopped to rotate, and the cotton swab is waited to enter the placing groove 45.
When the invention is used, before hemodialysis, the pipeline connected to the dialysis machine is required to be cleaned, and after the cleaning is finished, the bacterial residue in the pipeline is required to be detected, so that the bacteria in the connecting pipeline of the hemodialysis machine after the liquid A and the liquid B are connected are prevented from being simultaneously input into the human body, and bacterial infection is caused to a patient; before the sampling device is used, the mounting plate 11 is opened from the shell 1, and the collection cylinder 61 and the adjusting mechanism 5 are taken out from the clamping plate 13; rotating the rotary drum 52, the rotary drum 52 rotates by driving the external screw thread 63 arranged on the outer wall of the collecting drum 61, and the sliding block 62 slides on the inner wall of the sleeve 51, so that the sliding block 62 plays a guiding role and forces the rotary drum 52 to rotate so as to drive the collecting drum 61 to linearly move but not rotate; when the rotary drum 52 is rotated, the rotary drum 52 and the sleeve 51 are rotated relatively, so that the collecting drum 61 extends out of the inner drum 53, and a cotton swab is placed in the collecting cavity 613; when the drum 52 synchronously drives the inner drum 53 to rotate, the inner drum 53 rotates to drive a rotating rod 554 arranged on the inner wall of the inner drum to synchronously rotate, the rotating rod 554 rotates to abut against one side of the stop rod 553, and the rotating rod 554 drives the stop rod 553 to synchronously rotate until the rotating rod 554 rotates to a position separated from the stop rod 553 along with the inner drum 53; the rotating rod 554 continuously rotates along with the inner cylinder 53, when the inner cylinder 53 rotates for one circle, the rotating rod 554 pushes one stop lever 553 to rotate once, and when the stop lever 553 rotates, the stop lever 553 pushes the rotating rod 551 to rotate, and the rotating rod 551 rotates and then drives the second clamping tooth 552 to rotate; the second latch 552 rotates to drive the driven ring 571 to rotate, the driven ring 571 rotates to drive the pull rod 574 arranged on the inner part of the driven ring 571 to rotate, the end part of the pull rod 574 is connected with the sealing plate 575, and when the pull rod 574 pulls the sealing plate 575, the sealing plate 575 rotates around the rotating pin 576; until the plurality of closure plates 575 exit from the sealing aperture 58, at which point the cotton bud extends just outside the sleeve 51 through the sealing aperture 58; at this time, when the cotton swab is put into the centrifugal barrel 41 and the push rod 64 is pushed to move towards the inside of the collecting barrel 61, the piston 600 at the end part of the push rod 64 synchronously moves, the piston 600 pushes purified water below to extrude the turnover plate 69, the turnover plate 69 turns downwards along the plate groove 611 to impact water flow on the cotton swab through the screen 612, the cotton swab is separated from the inside of the collecting cavity 613, the whole cotton swab enters the inside of the centrifugal barrel 41, the detachable top cover 65 is arranged at the top part of the push rod 64, and purified water can be added into the inside of the inner cavity 66 after the top cover 65 is detached; when centrifugation is needed, the driving motor 72 rotates, the driving wheel 71 is driven to rotate by the rotation of the driving motor 72, the first clamping tooth 48 is driven to rotate by the driving wheel 71, the centrifugal plate 47 and the centrifugal cylinder 41 are connected together through screws, the centrifugal plate 47 drives the centrifugal cylinder 41 to synchronously rotate, so that the cotton swab inside the placing groove 45 is centrifuged, after centrifugation is finished, the fluorescent instrument 2 detects the centrifuged cotton swab, and when bacteria are detected, data are displayed on the display screen of the shell 1; medical staff can judge whether bacteria exceed the standard according to the data; when the detection is completed, it is necessary to kill bacteria, irradiate the centrifugal cylinder 41 and the centrifugal plate 47 with the sterilizing lamp 9, and kill the collection cylinder 61 placed inside the sterilizing tank 12 provided inside the housing 1. The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the foregoing examples, and that the foregoing description and description are merely illustrative of the principles of this invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims (1)

1. The bacteria detection sampling device for the hemodialysis machine comprises a shell (1), and is characterized in that an acquisition mechanism (6) is installed in the shell (1), and the acquisition mechanism (6) is used for acquiring bacteria; an adjusting mechanism (5) is arranged on the collecting mechanism (6), and the adjusting mechanism (5) is used for isolating collected bacteria from the outside; a centrifugal mechanism (4) is arranged in the shell (1), and the centrifugal mechanism (4) is used for centrifuging collected bacteria; the fluorescent lamp is characterized in that a fluorescent instrument (2) is arranged at the top of the shell (1), a centrifugal mechanism (4) is arranged below the fluorescent instrument (2), a driving mechanism (7) for driving the centrifugal mechanism (4) to rotate is arranged inside the shell (1), a waste barrel (8) is arranged below the centrifugal mechanism (4), and the waste barrel (8) is arranged inside the shell (1); a sterilizing lamp (9) is arranged in the shell (1), and the sterilizing lamp (9) is used for killing bacteria on the centrifugal mechanism (4) and the collecting mechanism (6);
The collecting mechanism (6) comprises a collecting barrel (61), a collecting cavity (613) is arranged at the end part of the collecting barrel (61), and a cotton swab for adsorbing bacteria is placed in the collecting cavity (613); the inside of the collection cylinder (61) is provided with a screen plate (612) positioned above the collection cavity (613), the inside of the collection cylinder (61) is provided with a plate groove (611), the upper part of the plate groove (611) is provided with a turnover plate (69), the inside of the collection cylinder (61) is provided with a stabilizer bar (610), and the stabilizer bar (610) is rotationally connected with the turnover plate (69);
The collecting mechanism (6) further comprises a push rod (64) arranged on the collecting barrel (61) in a sliding mode, a piston (600) positioned in the collecting barrel (61) is arranged at the end portion of the push rod (64), an inner cavity (66) is formed in the push rod (64), and the inner cavity (66) is used for storing purified water; two first one-way valves (67) are arranged on the rod wall of the push rod (64), and two second one-way valves (68) are arranged on the inner wall of the collection cylinder (61); the second one-way valve (68) is fed when the push rod (64) moves towards the outside of the collection cylinder (61), and the first one-way valve (67) is fed when the push rod (64) moves towards the inside of the collection cylinder (61);
The collecting mechanism (6) further comprises a sliding block (62) arranged on the outer wall of the collecting cylinder (61), and an external thread (63) is arranged on the outer part of the end part of the collecting cylinder (61);
The adjusting mechanism (5) comprises a rotary drum (52) which is arranged outside the collecting drum (61) and is in spiral connection with the collecting drum (61), and the rotary drum (52) is in rotary connection with the sleeve (51) through a support; the bottom end of the sleeve (51) is provided with a stabilizing plate (54), and the stabilizing plate (54) is fixed with the sleeve (51) through a plurality of fixing rods (56); an inner cylinder (53) is rotatably arranged in the sleeve (51), and the inner cylinder (53) synchronously rotates along with the rotation of the rotary cylinder (52); the middle part of the stabilizing plate (54) is provided with a sealing hole (58), the stabilizing plate (54) is provided with an opening and closing component (57) for sealing the sealing hole (58), the stabilizing plate (54) is provided with a pushing component (55), and the pushing component (55) is used for driving the opening and closing component (57) to open and close;
The pushing assembly (55) comprises a rotating rod (554) arranged on the inner wall of the inner cylinder (53), a rotating rod (551) is rotatably arranged on the stabilizing plate (54), and a plurality of stop rods (553) matched with the rotating rod (554) are arranged on the outer wall of the rotating rod (551); a second clamping tooth (552) is arranged on the outer surface of the rotating rod (551), and the second clamping tooth (552) is meshed with the opening and closing component (57);
The opening and closing assembly (57) comprises a driven ring (571) meshed with the second latch (552), a guide groove (572) is formed in the driven ring (571), a guide column (573) is arranged in the guide groove (572), the guide column (573) is fixed on the stabilizing plate (54), and the driven ring (571) rotates on the stabilizing plate (54); a pull rod (574) is rotatably arranged on the driven ring (571), a blocking plate (575) is rotatably connected to the end part of the pull rod (574), the blocking plate (575) is rotatably arranged on the stabilizing plate (54) through a rotary pin (576), and the blocking plate (575) is used for blocking the sealing hole (58);
The centrifugal mechanism (4) comprises a centrifugal cylinder (41) rotatably arranged in the shell (1), a rotary table (42) is arranged on the centrifugal cylinder (41), a bearing (44) is arranged on the rotary table (42), and the bearing (44) is rotatably arranged in the shell (1); a plurality of ratchets (43) which rotate unidirectionally are arranged on the outer wall of the rotary table (42); a plurality of placing grooves (45) are formed in the centrifugal barrel (41), and the placing grooves (45) are used for placing cotton swabs after bacteria are collected; a centrifugal plate (47) in spiral connection with the centrifugal cylinder (41) is arranged below the centrifugal cylinder (41), a feed opening (46) is arranged in the middle of the centrifugal plate (47), and a waste material cylinder (8) is arranged right below the feed opening (46); the outer wall of the centrifugal plate (47) is provided with a plurality of first clamping teeth (48);
The driving mechanism (7) comprises a driving wheel (71) meshed with the first latch (48), a motor (72) is arranged in the shell (1), the driving wheel (71) is arranged on an output shaft of the motor (72), and when the motor (72) rotates, the centrifugal plate (47) is driven to drive the centrifugal barrel (41) to synchronously rotate.
CN202410038858.0A 2024-01-11 2024-01-11 Bacteria detection sampling device for hemodialysis machine Active CN117551537B (en)

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Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112592814A (en) * 2020-12-23 2021-04-02 温州乔断信息科技有限公司 Microorganism periodic sampling detection device and sampling method
CN213266500U (en) * 2020-08-28 2021-05-25 四川大学华西第二医院 Bacterium detects sampling device
CN113640533A (en) * 2021-09-07 2021-11-12 刘洪� Veterinary livestock and poultry disease detection method
CN214730671U (en) * 2021-04-06 2021-11-16 长沙医学院 Novel blood clinical examination data is collected device
CN214830347U (en) * 2021-04-19 2021-11-23 南京华晟医学检验实验室有限公司 Brucellosis serum detection device
CN215961439U (en) * 2021-07-01 2022-03-08 中山大学附属第三医院(中山大学肝脏病医院) Hemodialysis filters replacement liquid sample thief
CN216645953U (en) * 2021-12-27 2022-05-31 上海长海医院 Wound surface repairing bacteria sampling device with built-in return structure
CN218710337U (en) * 2022-11-08 2023-03-24 广西桂科院康脉生物科技有限公司 Sampler for preparing stem cell sample
CN116473553A (en) * 2023-03-10 2023-07-25 江苏省中医药研究院 Hemodialysis sampling device

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN213266500U (en) * 2020-08-28 2021-05-25 四川大学华西第二医院 Bacterium detects sampling device
CN112592814A (en) * 2020-12-23 2021-04-02 温州乔断信息科技有限公司 Microorganism periodic sampling detection device and sampling method
CN214730671U (en) * 2021-04-06 2021-11-16 长沙医学院 Novel blood clinical examination data is collected device
CN214830347U (en) * 2021-04-19 2021-11-23 南京华晟医学检验实验室有限公司 Brucellosis serum detection device
CN215961439U (en) * 2021-07-01 2022-03-08 中山大学附属第三医院(中山大学肝脏病医院) Hemodialysis filters replacement liquid sample thief
CN113640533A (en) * 2021-09-07 2021-11-12 刘洪� Veterinary livestock and poultry disease detection method
CN216645953U (en) * 2021-12-27 2022-05-31 上海长海医院 Wound surface repairing bacteria sampling device with built-in return structure
CN218710337U (en) * 2022-11-08 2023-03-24 广西桂科院康脉生物科技有限公司 Sampler for preparing stem cell sample
CN116473553A (en) * 2023-03-10 2023-07-25 江苏省中医药研究院 Hemodialysis sampling device

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