CN112592814A

CN112592814A - Microorganism periodic sampling detection device and sampling method

Info

Publication number: CN112592814A
Application number: CN202011533582.1A
Authority: CN
Inventors: 石华平; 吉胜杰
Original assignee: Wenzhou Qiaoduan Information Technology Co ltd
Current assignee: Wenzhou Qiaoduan Information Technology Co ltd
Priority date: 2020-12-23
Filing date: 2020-12-23
Publication date: 2021-04-02

Abstract

The invention discloses a microorganism periodic sampling detection device and a sampling method, and the device comprises a culture mechanism, wherein the culture mechanism comprises an incubator, a transparent observation window is fixedly arranged on one side of the incubator, a culture dish is arranged at the bottom of an inner cavity of the incubator, a plugging mechanism is arranged at the upper end of the incubator, the plugging mechanism comprises an installation plate, the bottom of the installation plate is fixedly connected with the incubator, and a sampling box is arranged at the upper end of the installation plate; under the coordination action of the adjusting mechanism and the sampling mechanism, the invention provides convenience for the extraction of the culture solution containing the microorganisms, thereby facilitating the operation of a user and further realizing the regular sampling detection of the culture solution containing the microorganisms.

Description

Microorganism periodic sampling detection device and sampling method

Technical Field

The invention relates to the technical field of microorganism sampling detection, in particular to a microorganism regular sampling detection device and a sampling method.

Background

In the microorganism culture process, the cultured microorganism is required to be sampled and detected regularly, the mixed bacteria are caused to be mixed due to the nonstandard operation in the sampling process, the growth environment of the microorganism strains is influenced, the conventional microorganism regular sampling and detecting device has certain defects, if the sampling and detecting device used at present needs a manual handheld sampling mechanism to sample, a lot of inconvenience exists in the actual operation process, the device is not convenient for a user to use, and a plugging mechanism is not arranged between an incubator and a sampling box used in the conventional sampling and detecting device, but two groups are directly communicated, so that the air in the sampling box enters the incubator, the stability of the microorganism culture environment is further destroyed, the culture quality of the microorganism is reduced, in addition, a sterilization mechanism is not arranged in the conventional sampling box, and the bacteria contained in the external air are caused to be remained in the sampling box easily, therefore, a microorganism regular sampling detection device and a sampling method are provided for solving the problems.

Disclosure of Invention

The present invention is directed to a device and a method for sampling and detecting microorganisms periodically to solve the above problems.

In order to achieve the purpose, the invention provides the following technical scheme: a microorganism regular sampling detection device comprises a culture mechanism, the culture mechanism comprises an incubator, a transparent observation window is fixedly arranged on one side of the incubator, a culture dish is arranged at the bottom of the inner cavity of the incubator, the upper end of the incubator is provided with a plugging mechanism which comprises a mounting plate, the bottom of the mounting plate is fixedly connected with the incubator, the upper end of the mounting plate is provided with a sampling box, the incubator, the mounting plate and the sampling box can be communicated with each other, an adjusting mechanism is arranged in the inner cavity of the sampling box, the adjusting mechanism comprises a back-shaped frame, the back-shaped frame is slidably clamped in the inner cavity of the sampling box, the lower extreme fixed mounting who returns the type frame has sampling mechanism, the lower extreme one side of sampling case can be dismantled and be equipped with the slide glass that uses with the cooperation of sampling mechanism, the inner chamber top fixed mounting of sampling case has the sterilamp that the mirror image distributes.

As a preferred technical scheme of the invention, a plugging cavity is formed in the mounting plate, a rubber plugging plate is slidably clamped in the plugging cavity, a first lead screw is rotatably mounted at one end of the mounting plate, one end of the first lead screw extends into the plugging cavity and is inserted in an inner cavity of the rubber plugging plate in a threaded manner, the other end of the first lead screw penetrates through the mounting plate and is provided with a rotating wheel at the tail end thereof, second through grooves which are symmetrically distributed penetrate through the upper end and the lower end of the mounting plate, a first through groove which is matched with the second through grooves is formed in the middle of the top end of the incubator in a penetrating manner, a third through groove which is matched with the second through grooves is formed in one side of the bottom end of the sampling box in a penetrating manner, and the first through groove, the second through groove and the third through groove can be communicated with.

As a preferred technical scheme of the present invention, the adjusting mechanism includes a motor, the motor is fixedly installed at an upper end of one side of the sampling box, an output end of the motor is provided with a second lead screw, one end of the second lead screw penetrates through the sampling box and is rotatably connected with a side wall of an inner cavity of the sampling box, a moving block is inserted in the second lead screw in a threaded manner, one side of the moving block is provided with an L-shaped connecting plate, an air cylinder is fixedly installed on the L-shaped connecting plate, and an output end of the air cylinder penetrates through the L-shaped connecting plate and is fixedly connected with the back-shaped frame.

According to a preferable technical scheme of the invention, the tail end of the output end of the cylinder is provided with reinforcing members distributed in an array mode, and the bottoms of the reinforcing members are fixedly connected with the clip-shaped frame.

As a preferable technical scheme of the invention, one side of the moving block is fixedly provided with a guide ring, a guide rod matched with the guide ring is arranged in an inner cavity of the sampling box, and the guide ring is slidably clamped on the guide rod.

According to a preferable technical scheme of the invention, the sampling mechanism comprises a sampling sleeve, the sampling sleeve is fixedly mounted on the frame, a connecting piece is arranged at the upper end of the sampling sleeve, an electric telescopic rod is arranged on the connecting piece, a rubber plug disc is fixedly connected to the output end of the electric telescopic rod, and the rubber plug disc is slidably clamped in an inner cavity of the sampling sleeve.

As a preferable technical scheme of the invention, four groups of placing blocks are fixedly arranged on one side of the bottom of the inner cavity of the sampling box, a placing groove matched with a glass slide for use is formed in each placing block, and the glass slide can be slidably clamped in the placing groove.

As a preferred technical scheme of the invention, the lower end of one side of the sampling box is hinged with a sealing door, and one side of the sealing door is fixedly provided with a handle.

A sampling method of a microorganism periodic sampling detection device comprises the following steps:

step one, a user can carry out periodic sampling detection according to preset sampling time, when the sampling detection operation is required, the user can rotate the rotating wheel, so that the user drives the first screw rod to rotate, and further drives the rubber plugging plate to move to one side close to the rotating wheel, at the moment, the second through grooves formed in the upper side and the lower side of the mounting plate are gradually communicated, when one end of the rubber plugging plate is completely attached to the side wall of the inner cavity of the plugging cavity body, wherein the side wall of the inner cavity is close to one side of the rotating wheel, the first through groove, the second through groove and the third through groove are completely communicated, and then the user can stop rotating the rotating;

step two, a user can turn on the motor to drive the second lead screw to rotate, so that the moving block is driven to move towards one side close to the motor, the air cylinder is driven to move towards one side close to the motor through the L-shaped connecting plate, meanwhile, the air cylinder drives the returning frame to move synchronously, the sampling mechanism is driven to move towards one side close to the motor until the sampling mechanism moves to the position right above the third through groove, then the user can turn off the motor and turn on the air cylinder at the same time, the air cylinder pushes the returning frame to move downwards at the moment, so that the sampling mechanism is driven to move downwards until the bottom end of the sampling sleeve penetrates through the third through groove, the second through groove and the first through groove and contacts with the culture solution containing microorganisms in the culture dish, and at the moment, the user needs to turn off the air cylinder;

step three, a user needs to open the electric telescopic rod to pull the rubber plug disc upwards, so that a negative pressure environment is formed at the lower end of the inner cavity of the sampling sleeve, a culture solution containing microorganisms is further sucked into the inner cavity of the sampling sleeve, and when a proper amount of culture solution is contained in the inner cavity of the sampling sleeve, the user can close the electric telescopic rod;

step four, the user can open the air cylinder again to pull the return frame to move upwards so as to drive the sampling mechanism to move upwards until the sampling mechanism moves to the initial position, then the user can close the air cylinder and drive the motor to rotate reversely so as to drive the second lead screw to rotate reversely, further drive the moving block to move towards one side far away from the motor, meanwhile, the sampling mechanism moves synchronously with the moving block until the sampling mechanism moves right above the glass slide, and then the user can close the motor;

step five, the user can open the electric telescopic rod again to push the rubber plug disc to move downwards, so that the culture solution containing the microorganisms is led out of the sampling sleeve and laid on the glass slide until the culture solution in the sampling sleeve is completely laid on the glass slide, and then the user can close the electric telescopic rod;

step six, a user needs to rotate the rotating wheel reversely, so that the user drives the first screw rod to rotate reversely, and further drives the rubber plugging plate to move to one side far away from the rotating wheel until the rubber plugging plate moves to the initial position, and at the moment, the user can stop rotating the rotating wheel;

step seven, a user can pull the handle to open the sealing door, then the user can take the slide glass containing the culture solution out of the sampling box and carry out subsequent detection, and then the user can clamp a new slide glass in the placing groove again and close the sealing door;

step eight, the user can turn on the sterilizing lamp to perform sterilization operation until all bacteria remained in the sampling box are killed, so that the sterile environment is formed in the sampling box again, and then the user can turn off the sterilizing lamp;

step nine, when the time comes to the next sampling time, the user can repeat the steps to carry out the subsequent sampling detection operation.

Compared with the prior art, the invention has the beneficial effects that:

1. under the coordination action of the adjusting mechanism and the sampling mechanism, the invention provides convenience for the extraction of the culture solution containing the microorganisms, thereby facilitating the operation of a user and further realizing the regular sampling detection of the culture solution containing the microorganisms.

2. According to the invention, the plugging mechanism is arranged and used, so that the incubator can be isolated from the sampling box, and the contact of the air in the sampling box during the culture of the microorganisms is avoided, thereby ensuring the stability of the culture environment of the microorganisms and further improving the culture quality of the microorganisms.

3. The sterilization lamp is arranged and used, so that residual bacteria in the sampling box can be completely killed, a sterile environment is formed in the sampling box, and the condition that bacteria in the sampling box are mixed into the culture solution is effectively avoided.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of the backside structure of the present invention;

FIG. 3 is a schematic view of the structure of a culture mechanism according to the present invention;

FIG. 4 is a schematic structural view of the plugging mechanism of the present invention;

FIG. 5 is a schematic view of the internal structure of the sampling box of the present invention;

FIG. 6 is a schematic view of the internal structure of the sampling box of the present invention;

FIG. 7 is a schematic view of a part of the structure of the present invention;

FIG. 8 is a schematic view of the structure of the sampling mechanism of the present invention.

In the figure: 1. a culture mechanism; 11. an incubator; 12. a transparent viewing window; 13. a culture dish; 14. a first through groove; 2. a plugging mechanism; 21. mounting a plate; 22. plugging the cavity; 23. a rubber plugging plate; 24. a first lead screw; 25. a rotating wheel; 26. a second through groove; 3. a sampling box; 31. a third through groove; 32. a sealing door; 33. a handle; 4. an adjustment mechanism; 41. a back-shaped frame; 42. a motor; 43. a second lead screw; 44. a moving block; 45. an L-shaped connecting plate; 46. a cylinder; 47. a reinforcement; 48. a guide ring; 49. a guide bar; 5. a sampling mechanism; 51. a sampling sleeve; 52. a connecting member; 53. an electric telescopic rod; 54. a rubber plug disc; 6. a glass slide; 7. a sterilizing lamp; 8. placing the blocks; 81. and (6) placing the groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-8, the present invention provides a technical solution: a microorganism regular sampling detection device comprises a culture mechanism 1, the culture mechanism 1 comprises an incubator 11, a transparent observation window 12 is fixedly installed on one side of the incubator 11, the transparent observation window 12 is arranged and used to provide convenience for microorganism observation and subsequent sampling detection operation, a culture dish 13 is arranged at the bottom of an inner cavity of the incubator 11, a sealing mechanism is arranged on the incubator 11, a user can place raw materials required by microorganism culture in the culture dish 13 through the opening and closing sealing mechanism and perform the subsequent microorganism culture operation, a plugging mechanism 2 is arranged at the upper end of the incubator 11, the plugging mechanism 2 comprises a mounting plate 21, the bottom of the mounting plate 21 is fixedly connected with the incubator 11, a sampling box 3 is arranged at the upper end of the mounting plate 21, the incubator 11, the mounting plate 21 and the sampling box 3 can be mutually communicated, and an adjusting mechanism 4 is arranged in the inner cavity of the sampling box 3, adjustment mechanism 4 is including returning type frame 41, it establishes in the inner chamber of sampling case 3 to return type frame 41 slip card, the lower extreme fixed mounting who returns type frame 41 has sampling mechanism 5, sampling mechanism 5's principle is the same with the needle syringe principle, do not do too much repetitious here, the lower extreme one side of sampling case 3 can be dismantled and be equipped with the slide glass 6 that uses with the cooperation of sampling mechanism 5, the inner chamber top fixed mounting of sampling case 3 has the sterilamp 7 of mirror image distribution, sterilamp 7 can kill the whole of the inside remaining bacterium of sampling case 3, thereby make the inside sterile environment that forms of sampling case 3.

Plugging cavity 22 has been seted up to mounting panel 21's inside, the inside slip card of plugging cavity 22 is equipped with rubber shutoff board 23, mounting panel 21's one end is rotated and is installed first lead screw 24, in the shutoff cavity 22 that the one end of first lead screw 24 extended to and the screw thread was inserted and is established in the inner chamber of rubber shutoff board 23, the other end of first lead screw 24 runs through mounting panel 21 and is equipped with runner 25 at its end, the second through groove 26 of seting up symmetric distribution is run through with the lower extreme in the upper end of mounting panel 21, the first logical groove 14 of seting up with the cooperation of second through groove 26 and using is run through in the top middle part of incubator 11, the bottom one side of sampling box 3 runs through and sets up the third through groove 31 of using with the cooperation of second through groove 26, first logical groove 14, second through groove 26 and third through.

Adjustment mechanism 4 includes motor 42, and in this scheme: the type Y80M1-2 is preferably selected to the motor 42, the power supply interface of the motor is connected with a power supply system through a switch, the operation circuit of the motor 42 is a forward and reverse rotation control program of a conventional motor 42, the operation circuit is the conventional circuit, the circuits and the control related to the scheme are the prior art, redundant description is not performed herein, the motor 42 is fixedly installed at the upper end of one side of the sampling box 3, the output end of the motor 42 is provided with a second lead screw 43, one end of the second lead screw 43 penetrates through the sampling box 3 and is rotatably connected with the side wall of the inner cavity of the sampling box 3, a moving block 44 is inserted in the second lead screw 43 through threads, one side of the moving block 44 is provided with an L-shaped connecting plate 45, an air cylinder 46 is fixedly installed on the L-shaped connecting plate 45, and.

The end of cylinder 46 output is equipped with array distribution's reinforcement 47, and the bottom of reinforcement 47 and time type frame 41 fixed connection, and the setting of reinforcement 47 is used, has effectively improved the stability of returning type frame 41 and cylinder 46 output to be connected, thereby ensured the normal use of sampling mechanism 5.

A guide ring 48 is fixedly arranged on one side of the moving block 44, a guide rod 49 matched with the guide ring 48 for use is arranged in the inner cavity of the sampling box 3, the guide ring 48 is slidably clamped on the guide rod 49, and under the coordination action of the guide ring 48 and the guide rod 49, the moving block 44 is limited and guided.

Sampling mechanism 5 includes sampling sleeve 51, and sampling sleeve 51 fixed mounting is on returning type frame 41, and sampling sleeve 51's upper end is equipped with connecting piece 52, is equipped with electric telescopic handle 53 on the connecting piece 52, and electric telescopic handle 53's output fixedly connected with rubber plug disc 54, rubber plug disc 54 slip card are established in sampling sleeve 51's inner chamber.

One side fixed mounting of 3 inner chambers bottoms of sampling box has four groups to place piece 8, places and sets up on the piece 8 with the standing groove 81 that slide 6 cooperatees and use, and slide 6 can slide the card and establish in standing groove 81, and standing groove 81 sets up the use, has realized placing the stability of slide 6 to be convenient for lay the culture solution on slide 6.

The lower extreme of one side of sampling box 3 articulates there is sealing door 32, and one side fixed mounting of sealing door 32 has handle 33, and the setting of handle 33 is used, provides convenience for opening and shutting of sealing door 32.

step one, a user can perform periodic sampling detection according to preset sampling time, when the sampling detection operation is required, the user can rotate the rotating wheel 25, so that the user drives the first screw rod 24 to rotate, and further drives the rubber plugging plate 23 to move to one side close to the rotating wheel 25, at the moment, the second through grooves 26 formed in the upper side and the lower side of the mounting plate 21 are gradually communicated, when one end of the rubber plugging plate 23 is completely attached to the side wall of the inner cavity of the plugging cavity 22 close to one side of the rotating wheel 25, the first through groove 14, the second through groove 26 and the third through groove 31 are completely communicated, and then the user can stop rotating the rotating wheel 25;

step two, a user can open the motor 42 to drive the second screw rod 43 to rotate, so as to drive the moving block 44 to move towards one side close to the motor 42, and further drive the air cylinder 46 to move towards one side close to the motor 42 through the L-shaped connecting plate 45, at the same time, the air cylinder 46 drives the shaping frame 41 to synchronously move, and further drive the sampling mechanism 5 to move towards one side close to the motor 42 until the sampling mechanism 5 moves to the position right above the third through groove 31, then the user can close the motor 42 and simultaneously open the air cylinder 46, at this time, the air cylinder 46 pushes the shaping frame 41 to move downwards, so as to drive the sampling mechanism 5 to move downwards until the bottom end of the sampling sleeve 51 passes through the third through groove 31, the second through groove 26 and the first through groove 14 and contacts the culture solution containing microorganisms in the culture dish 13, and at this time, the user needs to close the air cylinder 46;

step three, the user needs to open the electric telescopic rod 53 to pull the rubber plug disc 54 upwards, so that a negative pressure environment is formed at the lower end of the inner cavity of the sampling sleeve 51, the culture solution containing the microorganisms is further sucked into the inner cavity of the sampling sleeve 51, and when a proper amount of culture solution is contained in the inner cavity of the sampling sleeve 51, the user can close the electric telescopic rod 53;

step four, the user can open the air cylinder 46 again to pull the return frame 41 to move upwards so as to drive the sampling mechanism 5 to move upwards until the sampling mechanism 5 moves to the initial position, then the user can close the air cylinder 46 and drive the motor 42 to rotate in the reverse direction so as to drive the second lead screw 43 to rotate in the reverse direction so as to drive the moving block 44 to move towards one side far away from the motor 42, meanwhile, the sampling mechanism 5 moves synchronously with the moving block 44 until the sampling mechanism 5 moves to the position right above the glass slide 6, and then the user can close the motor 42;

step five, the user can open the electric telescopic rod 53 again to drive the rubber plug disc 54 to move downwards, so that the culture solution containing the microorganisms is led out of the sampling sleeve 51 and is laid on the glass slide 6 until the culture solution in the sampling sleeve 51 is completely laid on the glass slide 6, and then the user can close the electric telescopic rod 53;

step six, the user needs to rotate the rotating wheel 25 in the reverse direction, so that the user drives the first screw rod 24 to rotate in the reverse direction, and further drives the rubber plugging plate 23 to move to one side far away from the rotating wheel 25 until the rubber plugging plate 23 moves to the initial position, and at the moment, the user can stop rotating the rotating wheel 25;

step seven, the user can pull the handle 33 to open the sealing door 32, then the user can take the slide 6 containing the culture solution out of the sampling box 3 and perform subsequent detection, and then the user can clamp a new slide 6 in the placing groove 81 again and close the sealing door 32;

step eight, the user can turn on the sterilizing lamp 7 to perform sterilization operation until all bacteria remained in the sampling box 3 are killed, so that the sterile environment is formed in the sampling box 3 again, and then the user can turn off the sterilizing lamp 7;

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A microorganism regular sampling detection device comprises a culture mechanism (1), and is characterized in that: the utility model discloses a novel culture medium, including train (11), one side fixed mounting of train (11) has transparent observation window (12), the inner chamber bottom of train (11) is equipped with culture dish (13), the upper end of train (11) is equipped with shutoff mechanism (2), shutoff mechanism (2) include mounting panel (21), the bottom and the train (11) fixed connection of mounting panel (21), the upper end of mounting panel (21) is equipped with sampling box (3), can communicate each other between train (11), mounting panel (21) and sampling box (3), be equipped with adjustment mechanism (4) in the inner chamber of sampling box (3), adjustment mechanism (4) are including returning type frame (41), returning type frame (41) slip the card and establishing in the inner chamber of sampling box (3), the lower extreme fixed mounting of returning type frame (41) has sampling mechanism (5), the utility model discloses a sterilization lamp, including sampling case (3), the lower extreme one side of sampling case (3) can be dismantled and be equipped with slide glass (6) that use with sampling mechanism (5) cooperation, the inner chamber top fixed mounting of sampling case (3) has sterilamp (7) that the mirror image distributes.

2. The periodic sampling and testing device for microorganisms according to claim 1, wherein: a plugging cavity (22) is arranged in the mounting plate (21), a rubber plugging plate (23) is arranged in the plugging cavity (22) in a sliding manner, a first lead screw (24) is rotatably arranged at one end of the mounting plate (21), one end of the first lead screw (24) extends into the plugging cavity (22) and is inserted into an inner cavity of the rubber plugging plate (23) in a threaded manner, the other end of the first lead screw (24) penetrates through the mounting plate (21) and is provided with a rotating wheel (25) at the tail end thereof, the upper end and the lower end of the mounting plate (21) penetrate through second through grooves (26) which are symmetrically distributed, a first through groove (14) matched with the second through groove (26) is formed in the middle of the top end of the incubator (11), a third through groove (31) matched with the second through groove (26) for use is formed in one side of the bottom end of the sampling box (3), the first through groove (14), the second through groove (26) and the third through groove (31) can be communicated with each other.

3. The periodic sampling and testing device for microorganisms according to claim 1, wherein: the adjusting mechanism (4) comprises a motor (42), the motor (42) is fixedly installed at the upper end of one side of the sampling box (3), a second lead screw (43) is arranged at the output end of the motor (42), one end of the second lead screw (43) penetrates through the sampling box (3) and is rotatably connected with the side wall of the inner cavity of the sampling box (3), a moving block (44) is inserted into the second lead screw (43) in a threaded mode, an L-shaped connecting plate (45) is arranged on one side of the moving block (44), an air cylinder (46) is fixedly installed on the L-shaped connecting plate (45), and the output end of the air cylinder (46) penetrates through the L-shaped connecting plate (45) and is fixedly connected with the loop-shaped frame (41).

4. A periodic sampling and testing device for microorganisms according to claim 3, wherein: the tail end of the output end of the air cylinder (46) is provided with reinforcing members (47) distributed in an array mode, and the bottoms of the reinforcing members (47) are fixedly connected with the die frame (41).

5. A periodic sampling and testing device for microorganisms according to claim 3, wherein: a guide ring (48) is fixedly mounted on one side of the moving block (44), a guide rod (49) matched with the guide ring (48) for use is arranged in an inner cavity of the sampling box (3), and the guide ring (48) is slidably clamped on the guide rod (49).

6. The periodic sampling and testing device for microorganisms according to claim 1, wherein: sampling mechanism (5) are including sampling sleeve (51), sampling sleeve (51) fixed mounting is on returning type frame (41), the upper end of sampling sleeve (51) is equipped with connecting piece (52), be equipped with electric telescopic handle (53) on connecting piece (52), the output fixedly connected with rubber stopper dish (54) of electric telescopic handle (53), rubber stopper dish (54) slip card is established in the inner chamber of sampling sleeve (51).

7. The periodic sampling and testing device for microorganisms according to claim 1, wherein: four groups of placing blocks (8) are fixedly mounted on one side of the bottom of the inner cavity of the sampling box (3), a placing groove (81) matched with the glass slide (6) for use is formed in each placing block (8), and the glass slide (6) can be slidably clamped in the placing groove (81).

8. The periodic sampling and testing device for microorganisms according to claim 1, wherein: one side lower extreme of sampling case (3) articulates there is sealing door (32), one side fixed mounting of sealing door (32) has handle (33).

9. A sampling method of a microorganism periodic sampling detection device is characterized by comprising the following steps:

step one, a user can carry out periodic sampling detection according to preset sampling time, when the sampling detection operation is required, the user can rotate the rotating wheel (25), so that the user drives the first screw rod (24) to rotate, and further drives the rubber plugging plate (23) to move to one side close to the rotating wheel (25), at the moment, the second through grooves (26) formed in the upper side and the lower side of the mounting plate (21) are gradually communicated, when one end of the rubber plugging plate (23) is completely attached to the side wall of the inner cavity of the plugging cavity (22) close to one side of the rotating wheel (25), the first through groove (14), the second through groove (26) and the third through groove (31) are completely communicated, and then the user can stop rotating the rotating wheel (25);

step two, a user can open the motor (42) to drive the second screw rod (43) to rotate, so as to drive the moving block (44) to move towards one side close to the motor (42), and further drive the air cylinder (46) to move towards one side close to the motor (42) through the L-shaped connecting plate (45), meanwhile, the air cylinder (46) drives the returning frame (41) to synchronously move, so as to drive the sampling mechanism (5) to move towards one side close to the motor (42) until the sampling mechanism (5) moves to the position right above the third through groove (31), then the user can close the motor (42) and open the air cylinder (46), at the moment, the air cylinder (46) pushes the returning frame (41) to move downwards, so as to drive the sampling mechanism (5) to move downwards until the bottom end of the sampling sleeve (51) penetrates through the third through groove (31), the second through groove (26) and the first through groove (14) and contacts with the culture solution containing microorganisms in the culture dish (13), at the moment, the user needs to close the air cylinder (46);

step three, a user needs to open the electric telescopic rod (53) to pull the rubber plug disc (54) upwards, so that a negative pressure environment is formed at the lower end of the inner cavity of the sampling sleeve (51), further, culture solution containing microorganisms is sucked into the inner cavity of the sampling sleeve (51), and when a proper amount of culture solution is contained in the inner cavity of the sampling sleeve (51), the user can close the electric telescopic rod (53);

fourthly, the user can open the air cylinder (46) again to pull the return frame (41) to move upwards so as to drive the sampling mechanism (5) to move upwards until the sampling mechanism (5) moves to the initial position, then the user can close the air cylinder (46) and drive the motor (42) to rotate reversely so as to drive the second lead screw (43) to rotate reversely, further drive the moving block (44) to move towards one side far away from the motor (42), meanwhile, the sampling mechanism (5) moves synchronously along with the moving block (44) until the sampling mechanism (5) moves right above the glass slide (6), and then the user can close the motor (42);

step five, the user can open the electric telescopic rod (53) again to drive the rubber plug disc (54) to move downwards, so that the culture solution containing the microorganisms is led out of the sampling sleeve (51) and is laid on the glass slide (6) until the culture solution in the sampling sleeve (51) is completely laid on the glass slide (6), and then the user can close the electric telescopic rod (53);

sixthly, the user needs to rotate the rotating wheel (25) in the reverse direction, so that the user drives the first screw rod (24) to rotate in the reverse direction, and further drives the rubber plugging plate (23) to move to one side far away from the rotating wheel (25) until the rubber plugging plate (23) moves to the initial position, and at the moment, the user can stop rotating the rotating wheel (25);

seventhly, a user can pull the handle (33) to open the sealing door (32), then the user can take the slide glass (6) containing the culture solution out of the sampling box (3) and carry out subsequent detection, and then the user can clamp a new slide glass (6) in the placing groove (81) again and close the sealing door (32);

step eight, the user can turn on the sterilizing lamp (7) to perform sterilization operation until all bacteria remained in the sampling box (3) are killed, so that the sterile environment is formed in the sampling box (3) again, and then the user can turn off the sterilizing lamp (7);