CN117159785A - Anti-inflammatory antibacterial hydrogel and preparation method thereof - Google Patents
Anti-inflammatory antibacterial hydrogel and preparation method thereof Download PDFInfo
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- CN117159785A CN117159785A CN202311099632.3A CN202311099632A CN117159785A CN 117159785 A CN117159785 A CN 117159785A CN 202311099632 A CN202311099632 A CN 202311099632A CN 117159785 A CN117159785 A CN 117159785A
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- 239000000017 hydrogel Substances 0.000 title claims abstract description 70
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- 238000002360 preparation method Methods 0.000 title abstract description 11
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 claims abstract description 156
- 239000002131 composite material Substances 0.000 claims abstract description 61
- 229910021536 Zeolite Inorganic materials 0.000 claims abstract description 52
- 239000010457 zeolite Substances 0.000 claims abstract description 52
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- 239000002086 nanomaterial Substances 0.000 claims abstract description 33
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- 238000000034 method Methods 0.000 claims description 21
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- YDDGKXBLOXEEMN-IABMMNSOSA-N chicoric acid Chemical compound O([C@@H](C(=O)O)[C@@H](OC(=O)\C=C\C=1C=C(O)C(O)=CC=1)C(O)=O)C(=O)\C=C\C1=CC=C(O)C(O)=C1 YDDGKXBLOXEEMN-IABMMNSOSA-N 0.000 claims description 15
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- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 claims description 12
- 239000011780 sodium chloride Substances 0.000 claims description 12
- QNILTEGFHQSKFF-UHFFFAOYSA-N n-propan-2-ylprop-2-enamide Chemical compound CC(C)NC(=O)C=C QNILTEGFHQSKFF-UHFFFAOYSA-N 0.000 claims description 10
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- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 5
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- LXBGSDVWAMZHDD-UHFFFAOYSA-N 2-methyl-1h-imidazole Chemical compound CC1=NC=CN1 LXBGSDVWAMZHDD-UHFFFAOYSA-N 0.000 description 2
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- RJQXTJLFIWVMTO-TYNCELHUSA-N Methicillin Chemical compound COC1=CC=CC(OC)=C1C(=O)N[C@@H]1C(=O)N2[C@@H](C(O)=O)C(C)(C)S[C@@H]21 RJQXTJLFIWVMTO-TYNCELHUSA-N 0.000 description 1
- FCDLCPWAQCPTKC-UHFFFAOYSA-N Rhein Chemical compound C1=CC=C2C(=O)C3=CC(C(=O)O)=CC(O)=C3C(=O)C2=C1O FCDLCPWAQCPTKC-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The application discloses an anti-inflammatory and antibacterial hydrogel and a preparation method thereof. Belongs to the technical field of medical hydrogel. The anti-inflammatory antibacterial hydrogel prepared by the application has an inner layer and an outer layer which are combined to form a structure, wherein the outer layer part is a composite membrane formed by crosslinking carboxymethyl cellulose, sericin and gelatin, and the inner layer part is prepared by doping a black scale-zeolite imidazole framework composite antibacterial nanomaterial into hydrogel formed by the reaction of hydroxypropyl chitosan, poly (N-isopropyl acrylamide) and elastin; the anti-inflammatory and antibacterial hydrogel prepared by the application not only solves the problem of secondary fixation of the traditional dressing, but also has the technical problems of poor toughness and low strength, and also has excellent skin adaptability and excellent antibacterial and anti-inflammatory properties.
Description
Technical Field
The application relates to the technical field of medical hydrogels, in particular to an anti-inflammatory and antibacterial hydrogel and a preparation method thereof.
Background
The skin is used as the most fragile tissue of the human body, is an important barrier for the human body, and protects the human body from the external environment. However, as the largest organ of the human body, the skin is often easily damaged, and once the wound is infected in the process of repairing the wound surface injury, adverse reactions such as inflammation, ulcer and the like can occur, and finally, amputation and even death of partial patients are caused. The traditional treatment method has some defects such as dressing pasting, drug resistance caused by frequent use of antibiotics, long healing time of chronic wounds, severe scars and the like.
The hydrogel has good biocompatibility, can meet the moist environment required by repairing wound injury by virtue of the porous structure and swelling performance of the hydrogel, absorbs exuded body fluid and can not isolate external gas, so that the reproduction of anaerobic bacteria is reduced, the healing of skin wound is further promoted, the problems of wound protection, inherent antibacterial property, biocompatibility and the like of the traditional dressing are solved, and the hydrogel is favored in the field of biological medicine and has potential to become an ideal material of wound dressing.
However, the hydrogel dressing commonly used in clinic at present has low strength and poor toughness, cannot meet the requirement of actual use, has the problem of insufficient antibacterial property, and cannot avoid scar formation.
Disclosure of Invention
In view of the problems in the prior art, the application provides the hydrogel which has high strength and good toughness, can be used for diminishing inflammation, resisting bacteria and promoting the healing of skin wounds and the preparation method thereof.
In order to achieve the above purpose, the application adopts the following technical scheme:
an anti-inflammatory and antibacterial hydrogel, which has a structure formed by combining an inner layer and an outer layer,
the outer layer part is a composite membrane formed by crosslinking carboxymethyl cellulose, sericin and gelatin, and the inner layer part is obtained by doping a composite antibacterial nanomaterial of black scale-zeolite imidazole framework into hydrogel formed by the reaction of hydroxypropyl chitosan, poly (N-isopropyl acrylamide) and elastin.
In a further technical scheme of the application, the preparation method of the anti-inflammatory and antibacterial hydrogel comprises the following steps:
step one: mixing hydrolyzed elastin and hydroxypropyl chitosan, adding N-isopropyl acrylamide aqueous solution, and stirring until hydrolyzed elastin and hydroxypropyl chitosan are completely dissolved to obtain N-isopropyl acrylamide-elastin-hydroxypropyl chitosan solution;
step two: adding 0.5M sodium chloride into the solution prepared in the step one, and stirring until the sodium chloride is fully dissolved;
step three: adding a cross-linking agent and an initiator into the solution obtained in the second step, fully stirring for 5min, carrying out ultrasonic treatment for 10min after N, N' -methylene bisacrylamide and ammonium persulfate are completely dissolved, adding a black scale-zeolite imidazole framework composite antibacterial nano material and a catalyst, and incubating for 30min at 37 ℃ to form a black scale-zeolite imidazole framework composite antibacterial nano material, and doping a hydrogel formed by the reaction of hydroxypropyl chitosan poly (N-isopropyl acrylamide) and elastin;
step four: mixing carboxymethyl cellulose, sericin, gelatin and glycerin, dissolving in distilled water, stirring and reacting for 24 hours at 50 ℃ to obtain a film forming liquid, uniformly spreading the film forming liquid, and then drying for 1 hour in a constant temperature environment at 40 ℃ to obtain the carboxymethyl cellulose-sericin-gelatin composite film;
step five: and (3) compositing the hydrogel prepared in the step (III) on the surface of a carboxymethyl cellulose-sericin-gelatin composite film by a layer-by-layer assembly casting method, so as to prepare the anti-inflammatory antibacterial hydrogel with an inner and outer double-layer composite structure.
In a further technical scheme of the application, the concentrations of the carboxymethyl cellulose, the sericin, the gelatin and the glycerol are respectively 1% (w/v) carboxymethyl cellulose, 0.8% (w/v) sericin, 1% (w/v) gelatin and 2% (w/v) glycerol.
In a further technical scheme of the application, the weight ratio of the hydrolyzed elastin to the hydroxypropyl chitosan is 1.5:1 wherein the N-isopropylacrylamide concentration is 26% (w/v).
In a further technical scheme of the application, the cross-linking agent, the initiator and the catalyst are respectively N, N' -methylene bisacrylamide, ammonium persulfate and tetramethyl ethylenediamine.
In a further technical scheme of the application, the black scale-zeolite imidazole framework composite antibacterial nanomaterial, the cross-linking agent, the initiator and the catalyst are added in the amounts of 30-40 parts, 3.5-4.5 parts, 2-3 parts and 3-4 parts by weight respectively.
In a further technical scheme of the application, in the further technical scheme of the application, the black scale-zeolite imidazole framework composite antibacterial nanomaterial is a mixture of black scale-zeolite imidazole framework nanocomposite and antibacterial material.
In a further technical scheme of the application, the black scale-zeolite imidazole framework composite antibacterial nanomaterial comprises 80-85 parts of black scale-zeolite imidazole framework nanocomposite and 15-20 parts of antibacterial material in parts by weight.
In a further technical scheme of the application, the antibacterial material is one or a combination of a plurality of rhein, dipotassium glycyrrhizinate and chicoric acid; preferably rhein, dipotassium glycyrrhizinate and chicoric acid which are mixed according to the mass ratio of 2:1:2, combining.
In a further technical scheme of the application, the black scale-zeolite imidazole framework nano-composite is synthesized by a method of growing zeolite imidazole framework-8 on black scales in situ.
According to the technical scheme, the application discloses an inner-outer double-layer anti-inflammatory antibacterial hydrogel dressing system, wherein the inner layer is a nanocomposite synthesized by doping a zeolite imidazole framework-8 on black scales in situ in the middle of hydrogel reacted based on hydroxypropyl chitosan poly (N-isopropyl acrylamide) and elastin, and meanwhile, natural antibacterial materials extracted from natural materials are doped, such as: the rhein, dipotassium glycyrrhizinate, chicoric acid and the like have the advantages that the antibacterial and anti-inflammatory effects are enhanced, the wound can be effectively repaired, the generation of scars is restrained, a compound formed by reacting 2d nano materials with a metal organic framework is doped into the middle of hydrogel, the technical problems of poor toughness and low strength of common hydrogel are effectively solved, the black scale nano compound has a sharp edge structure, bacterial cell membranes can be directly damaged, ROS (reactive oxygen species) in local bacteria can be increased under irradiation of visible light, bacteria can be killed, the biological compatibility is excellent, the black scale can play a long-term and stable role through compounding on the metal organic framework, the outer layer is a composite membrane formed by reacting and crosslinking carboxymethyl cellulose, sericin and gelatin, the composite membrane has excellent mechanical strength and strong tissue adhesiveness through a non-covalent crosslinking network, and can adapt to dynamic wound changes, and the membrane has good biodegradability.
The anti-inflammatory and antibacterial hydrogel with an inner layer is prepared by compounding the hydrogel dressing of the inner layer on the surface of a carboxymethyl cellulose-sericin-gelatin composite film through a layer-by-layer assembly casting method, the anti-inflammatory and antibacterial hydrogel with an inner and outer double-layer composite structure is prepared, the technical problems of secondary fixation of the traditional dressing, poor toughness and low strength of the hydrogel are solved, the hydrogel has excellent skin adaptability and excellent antibacterial and anti-inflammatory properties, has no stimulation effect, no anaphylactic phenomenon and no toxic or side effect on skin, has wide application prospect in the aspect of treating infectious inflammation as an external preparation, and can comprehensively solve the problems of wound pain, infectious inflammation and the like.
Detailed Description
The embodiment of the application discloses an anti-inflammatory antibacterial hydrogel which has a structure formed by compositing an inner layer and an outer layer, wherein the outer layer part is a composite membrane formed by crosslinking carboxymethyl cellulose, sericin and gelatin, and the inner layer part is formed by doping a black scale-zeolite imidazole framework composite antibacterial nano material into a hydrogel formed by reacting hydroxypropyl chitosan, poly (N-isopropyl acrylamide) and elastin.
The preparation method of the anti-inflammatory and antibacterial hydrogel comprises the following steps:
step one: mixing hydrolyzed elastin and hydroxypropyl chitosan, adding N-isopropyl acrylamide aqueous solution, and stirring until hydrolyzed elastin and hydroxypropyl chitosan are completely dissolved to obtain N-isopropyl acrylamide-elastin-hydroxypropyl chitosan solution;
step two: adding 0.5M sodium chloride into the solution prepared in the step one, and stirring until the sodium chloride is fully dissolved;
step three: adding a cross-linking agent and an initiator into the solution obtained in the second step, fully stirring for 5min, carrying out ultrasonic treatment for 10min after N, N' -methylene bisacrylamide and ammonium persulfate are completely dissolved, adding a black scale-zeolite imidazole framework composite antibacterial nano material and a catalyst, and incubating for 30min at 37 ℃ to form a black scale-zeolite imidazole framework composite antibacterial nano material, and doping a hydrogel formed by the reaction of hydroxypropyl chitosan poly (N-isopropyl acrylamide) and elastin;
step four: mixing carboxymethyl cellulose, sericin, gelatin and glycerin, dissolving in distilled water, stirring and reacting for 24 hours at 50 ℃ to obtain a film forming liquid, uniformly spreading the film forming liquid, and then drying for 1 hour in a constant temperature environment at 40 ℃ to obtain the carboxymethyl cellulose-sericin-gelatin composite film;
step five: and (3) compositing the hydrogel prepared in the step (III) on the surface of a carboxymethyl cellulose-sericin-gelatin composite film by a layer-by-layer assembly casting method, so as to prepare the anti-inflammatory antibacterial hydrogel with an inner and outer double-layer composite structure.
Wherein the concentrations of the carboxymethyl cellulose, the sericin, the gelatin and the glycerin are respectively 1% (w/v) carboxymethyl cellulose, 0.8% (w/v) sericin, 1% (w/v) gelatin and 2% (w/v) glycerin.
Wherein, the weight ratio of the hydrolyzed elastin to the hydroxypropyl chitosan to the N-isopropyl acrylamide aqueous solution is 1.5:1 wherein the N-isopropylacrylamide concentration is 26% (w/v).
Wherein the cross-linking agent, the initiator and the catalyst are respectively N, N' -methylene bisacrylamide, ammonium persulfate and tetramethyl ethylenediamine.
Wherein, the black scale-zeolite imidazole framework composite antibacterial nano material, the cross-linking agent, the initiator and the catalyst are respectively added in the weight parts of 30-40 parts, 3.5-4.5 parts, 2-3 parts and 3-4 parts.
Wherein the black scale-zeolite imidazole framework composite antibacterial nanomaterial is a mixture of black scale-zeolite imidazole framework nanocomposite and antibacterial material; the black scale-zeolite imidazole framework composite antibacterial nano material consists of 80-85 parts of black scale-zeolite imidazole framework nano composite and 15-20 parts of antibacterial material according to parts by weight.
Wherein the antibacterial material is selected from one or more of rhein, dipotassium glycyrrhizinate and chicoric acid; such as: two-by-two combinations (rhein and dipotassium glycyrrhizinate, rhein and chicoric acid or dipotassium glycyrrhizinate and chicoric acid, wherein the mass ratio of the rhein to the chicoric acid is 1:2-3-4 when two-by-two combinations, such as three-three combinations (rhein, dipotassium glycyrrhizinate and chicoric acid), wherein the mass ratio of the rhein to the dipotassium glycyrrhizinate to the chicoric acid is 2:1:2 when three-three combinations are performed, and preferably, rhein, dipotassium glycyrrhizinate and chicoric acid are combined according to the mass ratio of 2:1:2.
Wherein, the black scale-zeolite imidazole framework nano-composite is synthesized by a method of growing zeolite imidazole framework-8 on black scale in situ; the specific synthesis method comprises the following steps: dispersing polyvinylpyrrolidone with methanol, adding black scales into the solution, performing ultrasonic treatment for 30min, and adding 2-methylimidazole into the solution to obtain solution A; dissolving hexahydrate and zinc nitrate in methanol to obtain solution B; and (3) mixing the solution A and the solution B, magnetically stirring at a rotating speed of 1200r/min for 15min, standing for 24h, centrifugally cleaning with methanol, and drying the crystals in a baking oven at 60 ℃ until the weight is constant, thus obtaining the black scale-zeolite imidazole framework nano-composite.
The following description of the technical solutions in the embodiments of the present application will be clear and complete, and it is obvious that the described embodiments are only some embodiments of the present application, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
Example 1
An anti-inflammatory and antibacterial hydrogel, which has a structure formed by combining an inner layer and an outer layer,
the outer layer part is a composite membrane formed by crosslinking carboxymethyl cellulose, sericin and gelatin, and the inner layer part is obtained by doping a composite antibacterial nanomaterial of black scale-zeolite imidazole framework into hydrogel formed by the reaction of hydroxypropyl chitosan, poly (N-isopropyl acrylamide) and elastin.
The preparation method of the anti-inflammatory and antibacterial hydrogel comprises the following steps:
step one: the hydrolyzed elastin and hydroxypropyl chitosan are mixed according to the weight ratio of 1.5: mixing in proportion 1, adding 26% (w/v) of N-isopropyl acrylamide aqueous solution, and stirring until hydrolyzed elastin and hydroxypropyl chitosan are completely dissolved to obtain N-isopropyl acrylamide-elastin-hydroxypropyl chitosan solution;
step two: adding 0.5M sodium chloride into the solution prepared in the step one, and stirring until the sodium chloride is fully dissolved;
step three: adding 3.5 parts of N, N '-methylene bisacrylamide and 2 parts of ammonium persulfate into the solution obtained in the step two according to parts by weight, fully stirring for 5min until the N, N' -methylene bisacrylamide and the ammonium persulfate are completely dissolved, performing ultrasonic treatment for 10min, adding 30 parts of black scale-zeolite imidazole framework composite antibacterial nano material and 3 parts of tetramethyl ethylenediamine, and incubating for 30min at 37 ℃ to form a black scale-zeolite imidazole framework composite antibacterial nano material, wherein the black scale-zeolite imidazole framework composite antibacterial nano material is doped with hydrogel formed by the reaction of hydroxypropyl chitosan poly (N-isopropyl acrylamide) and elastin; the black scale-zeolite imidazole framework composite antibacterial nanomaterial comprises 80-85 parts of black scale-zeolite imidazole framework nanocomposite and 15 parts of antibacterial material in parts by weight; the antibacterial material is prepared from rhein and dipotassium glycyrrhizinate according to a weight ratio of 1:3, composing;
step four: mixing 1% (w/v) carboxymethyl cellulose, 0.8% (w/v) sericin, 1% (w/v) gelatin and 2% (w/v) glycerin, dissolving in distilled water, stirring and reacting for 24 hours at 50 ℃ to obtain a film forming liquid, uniformly tiling the film forming liquid, and then drying for 1 hour in a constant temperature environment at 40 ℃ to obtain the carboxymethyl cellulose-sericin-gelatin composite film;
step five: and (3) compositing the hydrogel prepared in the step (III) on the surface of a carboxymethyl cellulose-sericin-gelatin composite film by a layer-by-layer assembly casting method, so as to prepare the anti-inflammatory antibacterial hydrogel with an inner and outer double-layer composite structure.
Example 2
An anti-inflammatory and antibacterial hydrogel, which has a structure formed by combining an inner layer and an outer layer,
the outer layer part is a composite membrane formed by crosslinking carboxymethyl cellulose, sericin and gelatin, and the inner layer part is obtained by doping a composite antibacterial nanomaterial of black scale-zeolite imidazole framework into hydrogel formed by the reaction of hydroxypropyl chitosan, poly (N-isopropyl acrylamide) and elastin.
The preparation method of the anti-inflammatory and antibacterial hydrogel comprises the following steps:
step one: the hydrolyzed elastin and hydroxypropyl chitosan are mixed according to the weight ratio of 1.5: mixing in proportion 1, adding 26% (w/v) of N-isopropyl acrylamide aqueous solution, and stirring until hydrolyzed elastin and hydroxypropyl chitosan are completely dissolved to obtain N-isopropyl acrylamide-elastin-hydroxypropyl chitosan solution;
step two: adding 0.5M sodium chloride into the solution prepared in the step one, and stirring until the sodium chloride is fully dissolved;
step three: adding 4.5 parts by weight of N, N '-methylene bisacrylamide and 3 parts by weight of ammonium persulfate into the solution obtained in the step two, fully stirring for 5min until the N, N' -methylene bisacrylamide and the ammonium persulfate are completely dissolved, performing ultrasonic treatment for 10min, adding 40 parts by weight of black scale-zeolite imidazole framework composite antibacterial nano material and 4 parts by weight of tetramethyl ethylenediamine, and incubating for 30min at 37 ℃ to form a black scale-zeolite imidazole framework composite antibacterial nano material, wherein the black scale-zeolite imidazole framework composite antibacterial nano material is doped with hydrogel formed by the reaction of hydroxypropyl chitosan poly (N-isopropyl acrylamide) and elastin; the black scale-zeolite imidazole framework composite antibacterial nanomaterial comprises, by weight, 85 parts of black scale-zeolite imidazole framework nanocomposite and 15 parts of antibacterial material; the antibacterial material is prepared from dipotassium glycyrrhizinate and chicoric acid according to a weight ratio of 2:4, the composition is formed;
step four: mixing 1% (w/v) carboxymethyl cellulose, 0.8% (w/v) sericin, 1% (w/v) gelatin and 2% (w/v) glycerin, dissolving in distilled water, stirring and reacting for 24 hours at 50 ℃ to obtain a film forming liquid, uniformly tiling the film forming liquid, and then drying for 1 hour in a constant temperature environment at 40 ℃ to obtain the carboxymethyl cellulose-sericin-gelatin composite film;
step five: and (3) compositing the hydrogel prepared in the step (III) on the surface of a carboxymethyl cellulose-sericin-gelatin composite film by a layer-by-layer assembly casting method, so as to prepare the anti-inflammatory antibacterial hydrogel with an inner and outer double-layer composite structure.
Example 3
An anti-inflammatory and antibacterial hydrogel, which has a structure formed by combining an inner layer and an outer layer,
the outer layer part is a composite membrane formed by crosslinking carboxymethyl cellulose, sericin and gelatin, and the inner layer part is obtained by doping a composite antibacterial nanomaterial of black scale-zeolite imidazole framework into hydrogel formed by the reaction of hydroxypropyl chitosan, poly (N-isopropyl acrylamide) and elastin.
The preparation method of the anti-inflammatory and antibacterial hydrogel comprises the following steps:
step one: the hydrolyzed elastin and hydroxypropyl chitosan are mixed according to the weight ratio of 1.5: mixing in proportion 1, adding 26% (w/v) of N-isopropyl acrylamide aqueous solution, and stirring until hydrolyzed elastin and hydroxypropyl chitosan are completely dissolved to obtain N-isopropyl acrylamide-elastin-hydroxypropyl chitosan solution;
step two: adding 0.5M sodium chloride into the solution prepared in the step one, and stirring until the sodium chloride is fully dissolved;
step three: adding 4 parts of N, N '-methylene bisacrylamide and 2.5 parts of ammonium persulfate into the solution obtained in the step two according to parts by weight, fully stirring for 5min until the N, N' -methylene bisacrylamide and the ammonium persulfate are completely dissolved, performing ultrasonic treatment for 10min, adding 35 parts of black scale-zeolite imidazole framework composite antibacterial nano material and 3.5 parts of tetramethyl ethylenediamine, and incubating for 30min at 37 ℃ to form a hydrogel formed by the reaction of hydroxypropyl chitosan poly (N-isopropyl acrylamide) and elastin; the black scale-zeolite imidazole framework composite antibacterial nanomaterial comprises 82 parts of black scale-zeolite imidazole framework nanocomposite and 18 parts of antibacterial material in parts by weight; the antibacterial material is prepared from rhein, dipotassium glycyrrhizinate and chicoric acid according to a mass ratio of 2:1:2, composing;
step four: mixing 1% (w/v) carboxymethyl cellulose, 0.8% (w/v) sericin, 1% (w/v) gelatin and 2% (w/v) glycerin, dissolving in distilled water, stirring and reacting for 24 hours at 50 ℃ to obtain a film forming liquid, uniformly tiling the film forming liquid, and then drying for 1 hour in a constant temperature environment at 40 ℃ to obtain the carboxymethyl cellulose-sericin-gelatin composite film;
step five: and (3) compositing the hydrogel prepared in the step (III) on the surface of a carboxymethyl cellulose-sericin-gelatin composite film by a layer-by-layer assembly casting method, so as to prepare the anti-inflammatory antibacterial hydrogel with an inner and outer double-layer composite structure.
Example 4
The black scale-zeolite imidazole framework nanocomposite described in examples 1-3 above was synthesized by growing zeolite imidazole framework-8 in situ on black scale; the specific synthesis method comprises the following steps: dispersing polyvinylpyrrolidone with methanol, adding black scales into the solution, performing ultrasonic treatment for 30min, and adding 2-methylimidazole into the solution to obtain solution A; dissolving hexahydrate and zinc nitrate in methanol to obtain solution B; and (3) mixing the solution A and the solution B, magnetically stirring at a rotating speed of 1200r/min for 15min, standing for 24h, centrifugally cleaning with methanol, and drying the crystals in a baking oven at 60 ℃ until the weight is constant, thus obtaining the black scale-zeolite imidazole framework nano-composite.
Experimental example
30 mice with the age of 10-12 weeks are selected and randomly divided into 3 groups, each group is divided into half male and half female, 10 mice are used for establishing a skin defect infection model, namely, a full-layer skin defect with the diameter of about 0.8cm is formed on the back of the mice, 0.05ml of 2X 108CFU/ml methicillin-resistant staphylococcus aureus bacterial liquid is injected, after the bacterial liquid is completely absorbed and dried, the anti-inflammatory antibacterial hydrogel prepared in the embodiment 3 is respectively used for covering a wound surface to serve as an experimental group, a blank control group is arranged, the defect infection model is not treated, and a wound is covered by 3M Tegaderm application. In addition, a positive control group was set, and the wound was covered with 3M Tegaderm dressing, which was applied to the affected area of the defect with the BAIDOUNGMUpirocin ointment.
Experimental results: referring to the blank control group, the sterilization rate of the experimental group and the positive control group to which the anti-inflammatory sterilization hydrogel of the application is applied is 75.4% and 72.1% respectively on day 3; the sterilization rate on day 8 was 98.1% and 97.1%. The wound healing rate results of the anti-inflammatory bactericidal hydrogel and the mupirocin positive control show that the time required for the wound surface average wound healing of the wound surface of the mice in the experimental group, the wound surface of the mice in the blank control group and the mupirocin positive control group to be 9.5 days, 14.8 days and 20.1 days respectively.
Conclusion: the anti-inflammatory and bactericidal hydrogel prepared by the application has excellent promoting effect on wound healing and good applicability.
In the present specification, each embodiment is described in a progressive manner, and each embodiment is mainly described in a different point from other embodiments, and identical and similar parts between the embodiments are all enough to refer to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the application. Thus, the present application is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (8)
1. An anti-inflammatory and antibacterial hydrogel, characterized in that: the anti-inflammatory antibacterial hydrogel has an inner layer and an outer layer which are combined to form a structure, wherein the outer layer part is a composite membrane formed by crosslinking carboxymethyl cellulose, sericin and gelatin, and the inner layer part is formed by doping a black scale-zeolite imidazole framework composite antibacterial nano material into hydrogel formed by the reaction of hydroxypropyl chitosan, poly (N-isopropyl acrylamide) and elastin;
the black scale-zeolite imidazole framework composite antibacterial nanomaterial is a mixture of black scale-zeolite imidazole framework nanocomposite and antibacterial material;
the antibacterial material is one or a combination of a plurality of rhein, dipotassium glycyrrhizinate and chicoric acid;
the black scale-zeolite imidazole framework nano-composite is synthesized by a method of growing zeolite imidazole framework-8 on black scales in situ.
2. The anti-inflammatory and antibacterial hydrogel according to claim 1, wherein: the black scale-zeolite imidazole framework composite antibacterial nanomaterial consists of 80-85 parts of black scale-zeolite imidazole framework nanocomposite and 15-20 parts of antibacterial material.
3. The anti-inflammatory and antibacterial hydrogel according to claim 1, wherein: the antibacterial material is rhein, dipotassium glycyrrhizinate and chicoric acid, and the mass ratio of the rhein to the dipotassium glycyrrhizinate to the chicoric acid is 2:1:2, combining.
4. A method for preparing the anti-inflammatory and antibacterial hydrogel according to any one of claims 1 to 3, comprising the steps of:
step one: mixing hydrolyzed elastin and hydroxypropyl chitosan, adding N-isopropyl acrylamide aqueous solution, and stirring until hydrolyzed elastin and hydroxypropyl chitosan are completely dissolved to obtain N-isopropyl acrylamide-elastin-hydroxypropyl chitosan solution;
step two: adding 0.5M sodium chloride into the solution prepared in the step one, and stirring until the sodium chloride is fully dissolved;
step three: adding a cross-linking agent and an initiator into the solution obtained in the second step, fully stirring for 5min, carrying out ultrasonic treatment for 10min after N, N' -methylene bisacrylamide and ammonium persulfate are completely dissolved, adding a black scale-zeolite imidazole framework composite antibacterial nano material and a catalyst, and incubating for 30min at 37 ℃ to form a black scale-zeolite imidazole framework composite antibacterial nano material, and doping a hydrogel formed by the reaction of hydroxypropyl chitosan poly (N-isopropyl acrylamide) and elastin;
step four: mixing carboxymethyl cellulose, sericin, gelatin and glycerin, dissolving in distilled water, stirring and reacting for 24 hours at 50 ℃ to obtain a film forming liquid, uniformly spreading the film forming liquid, and then drying for 1 hour in a constant temperature environment at 40 ℃ to obtain the carboxymethyl cellulose-sericin-gelatin composite film;
step five: and (3) compositing the hydrogel prepared in the step (III) on the surface of a carboxymethyl cellulose-sericin-gelatin composite film by a layer-by-layer assembly casting method, so as to prepare the anti-inflammatory antibacterial hydrogel with an inner and outer double-layer composite structure.
5. The method for preparing the anti-inflammatory and antibacterial hydrogel according to claim 4, wherein the method comprises the following steps: the carboxymethyl cellulose, sericin, gelatin and glycerin concentrations in step four were 1% (w/v) carboxymethyl cellulose, 0.8% (w/v) sericin, 1% (w/v) gelatin and 2% (w/v) glycerin, respectively.
6. The method for preparing the anti-inflammatory and antibacterial hydrogel according to claim 4, wherein the method comprises the following steps: in the first step, the weight ratio of the hydrolyzed elastin to the hydroxypropyl chitosan is 1.5:1, a step of; the N-isopropylacrylamide concentration was 26% (w/v).
7. The method for preparing the anti-inflammatory and antibacterial hydrogel according to claim 4, wherein the method comprises the following steps: in the third step, the cross-linking agent, the initiator and the catalyst are respectively N, N' -methylene bisacrylamide, ammonium persulfate and tetramethyl ethylenediamine.
8. The method for preparing the anti-inflammatory and antibacterial hydrogel according to claim 4, wherein the method comprises the following steps: the black scale-zeolite imidazole framework composite antibacterial nano material, the cross-linking agent, the initiator and the catalyst are respectively added in the weight portions of 30-40 parts, 3.5-4.5 parts, 2-3 parts and 3-4 parts.
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011036622A (en) * | 2009-08-12 | 2011-02-24 | Nagasuna Mayu Inc | Method of manufacturing film containing silk fibroin or sericin |
| CN111558051A (en) * | 2020-07-08 | 2020-08-21 | 中国科学院深圳先进技术研究院 | Composite nano-microsphere with rapid mucus penetration effect and preparation method and application thereof |
| CN112957343A (en) * | 2021-02-10 | 2021-06-15 | 浙江工业大学 | Protein @ ZIF-8N nano material and preparation and application thereof |
| CN113150362A (en) * | 2021-04-02 | 2021-07-23 | 淮阴工学院 | Preparation method and application of porous hydrogel integrating capturing and killing of bacteria |
| CN113181421A (en) * | 2021-05-12 | 2021-07-30 | 广州贝奥吉因生物科技股份有限公司 | Hydrogel wound dressing with antibacterial and immunoregulation functions and preparation method thereof |
| CN114045172A (en) * | 2021-09-15 | 2022-02-15 | 青海大学 | Preparation method of novel hydrophilic black phosphorus quantum dot-zeolite imidazolate framework fluorescent probe material |
| CN114479479A (en) * | 2022-02-23 | 2022-05-13 | 上海忒尔苏斯环境科技合伙企业(有限合伙) | Preparation method for synthesizing black phosphorus-metal organic framework composite material in limited domain |
| CN115297905A (en) * | 2020-01-20 | 2022-11-04 | 皇家墨尔本理工大学 | Antimicrobial coatings |
| CN115887647A (en) * | 2022-11-01 | 2023-04-04 | 石河子大学 | Temperature-sensitive black phosphorus hydrogel for promoting wound healing and preparation method thereof |
| CN116440317A (en) * | 2023-04-06 | 2023-07-18 | 福州大学 | Photothermal antibacterial hydrogel and preparation method thereof |
-
2023
- 2023-08-28 CN CN202311099632.3A patent/CN117159785A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011036622A (en) * | 2009-08-12 | 2011-02-24 | Nagasuna Mayu Inc | Method of manufacturing film containing silk fibroin or sericin |
| CN115297905A (en) * | 2020-01-20 | 2022-11-04 | 皇家墨尔本理工大学 | Antimicrobial coatings |
| CN111558051A (en) * | 2020-07-08 | 2020-08-21 | 中国科学院深圳先进技术研究院 | Composite nano-microsphere with rapid mucus penetration effect and preparation method and application thereof |
| CN112957343A (en) * | 2021-02-10 | 2021-06-15 | 浙江工业大学 | Protein @ ZIF-8N nano material and preparation and application thereof |
| CN113150362A (en) * | 2021-04-02 | 2021-07-23 | 淮阴工学院 | Preparation method and application of porous hydrogel integrating capturing and killing of bacteria |
| CN113181421A (en) * | 2021-05-12 | 2021-07-30 | 广州贝奥吉因生物科技股份有限公司 | Hydrogel wound dressing with antibacterial and immunoregulation functions and preparation method thereof |
| CN114045172A (en) * | 2021-09-15 | 2022-02-15 | 青海大学 | Preparation method of novel hydrophilic black phosphorus quantum dot-zeolite imidazolate framework fluorescent probe material |
| CN114479479A (en) * | 2022-02-23 | 2022-05-13 | 上海忒尔苏斯环境科技合伙企业(有限合伙) | Preparation method for synthesizing black phosphorus-metal organic framework composite material in limited domain |
| CN115887647A (en) * | 2022-11-01 | 2023-04-04 | 石河子大学 | Temperature-sensitive black phosphorus hydrogel for promoting wound healing and preparation method thereof |
| CN116440317A (en) * | 2023-04-06 | 2023-07-18 | 福州大学 | Photothermal antibacterial hydrogel and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 焦体峰等: "《纳米复合水凝胶》", 31 October 2022, 中国建材工业出版社, pages: 7 * |
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