CN103301504A - Preparation method of gamma-polyglutamate/sericin hydrogel dressing - Google Patents
Preparation method of gamma-polyglutamate/sericin hydrogel dressing Download PDFInfo
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- CN103301504A CN103301504A CN2013102644545A CN201310264454A CN103301504A CN 103301504 A CN103301504 A CN 103301504A CN 2013102644545 A CN2013102644545 A CN 2013102644545A CN 201310264454 A CN201310264454 A CN 201310264454A CN 103301504 A CN103301504 A CN 103301504A
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Abstract
The invention discloses a preparation method of a gamma-polyglutamate/sericin hydrogel dressing. In the preparation method, gamma-polyglutamate and sericin serve as main raw materials; and ethylene glycol diglycidyl ether serves as a cross-linking agent. The method for preparing the hydrogel dressing comprises the following steps: dissolving the gamma-polyglutamate with the concentration of 15 to 30 percent and a certain mass of sericin in deionized water; adding 16 to 65 percent of the molar weight of the ethylene glycol diglycidyl ether into the gamma-polyglutamate; stirring uniformly at room temperature; injecting the solution into a homemade mould; and reacting for 5 hours in water bath at the temperature of 40 to 80 DEG C to obtain the gamma-polyglutamate/sericin hydrogel dressing. The hydrogel dressing is easy to prepare, short in time, high in efficiency and large in large liquid adsorption amount and has high biocompatibility and biodegradability.
Description
[technical field]: the present invention relates to a kind of preparation method of biocompatible hydrogel dressing, be specifically a kind of be the method that primary raw material prepares aerogel dressing with gamma-polyglutamic acid-, sericin.
[background technology]: conventional medical dressing mainly comprises materials such as gauze, binder, and is current more extensive in the skin trauma clinical practice.This dressing source is wide, material is soft, cost is low, and absorbing wound exudate and seepage-proof liquid, to gather ability stronger, though can play certain protective role to wound, the healing of wound there is not facilitation.When changing wound dressing, the granulation tissue president of wound in gauze and inter-adhesive with it, thereby cause the wound secondary damage, prolong the healing time of wound surface.Because traditional medical accessory does not have antibiotic property, so generally can use antibiotic in the wound, add antibiotic and cause that easily the wound bacterial drug resistance strengthens, wound subjects to bacterial infection and is difficult to healing.
Britain scientist Gcorge doctor Winter found in 1962, and moist environment is conducive to wound healing.Therefore, various new-type dressing have obtained widely development based on this thought, as aerogel dressing etc.Aerogel dressing and desirable dressing performance are more approaching, aerogel dressing is except having excellent biological compatibility, also has the transparent wound situation that is easy to check own, can a large amount of penetrating fluids of fast Absorption, thus provide moistening environment to promote advantages such as granulation tissue growth for the quickly-healing of wound.Even wound surface injustice, aerogel dressing also can fit tightly with it and can not adhere to wound, reduced the probability of bacterial infection.
Gamma-polyglutamic acid-(as Fig. 1) is a kind of macromolecular homopolymer, passes through γ-amido link be combined into by L-glutamic acid and D-glutamic acid.γ-PGA as biological product has the following advantages: (1) biodegradability and catabolite are to human body and environmentally friendly; (2) good water solublity can obtain cleaning tasteless solution; (3) and cross-linking agent easily be cross-linked into well behaved hydrogel; (4) superpower adsorptivity can Adsorption of Heavy Metal Ions, is of value to the friendly type of built environment society.
Sericin is a kind of protein that is the sericin shape, include 18 kinds of amino acid needed by human body, appropriate aminoacid ratio is conducive to people's epidermis fibroblastic growth, propagation, promote wound to repair fast, and have calm the nerves, the magical effect of skin moistening and balance human skin, have characteristics such as easy dissolving, good water absorption, antioxidation, correctability, have good exploitation potential at food, cosmetics, biomaterial etc.
Consult domestic and foreign literature and find the preparation method of gamma-polyglutamic acid-hydrogel, comprise physical crosslinking method and chemical crosslink technique.Wherein, it is simple that the crosslinked method of physics radiation prepares the hydrogel production process, and mild condition need not to add cross-linking agent, need not repurity and handle.But side reaction easily takes place in crosslinking with radiation.
[summary of the invention]: in order to overcome above-mentioned the deficiencies in the prior art, the invention provides a kind of gamma-polyglutamic acid-/sericin aerogel dressing.Sericin is blended into is dissolved in the water around the gamma-polyglutamic acid-molecule, the sericin side chain contains a large amount of hydrophilic groups, and serine helps immunoglobulin and production of antibodies to wound healing and antibacterial very big facilitation arranged in the sericin.It has good absorbency, biocompatibility and biological degradability, also has certain bacteriostasis.
Advantage of the present invention and good effect:
The technical solution adopted in the present invention is: a kind of gamma-polyglutamic acid-/sericin aerogel dressing comprises at least: gamma-polyglutamic acid-, sericin, Ethylene glycol diglycidyl ether, deionized water, and the mass percent of each component is:
The method for preparing aerogel dressing comprises: be that 15%～30% gamma-polyglutamic acid-and the sericin of certain mass are dissolved in the deionized water with mass concentration, press the Ethylene glycol diglycidyl ether that 16%～65% of gamma-polyglutamic acid-monomer molar amount adds respective amount, stir under the room temperature, the solution injection is reacted 5h from molding jig in 40～80 ℃ of water-baths, can make gamma-polyglutamic acid-/sericin aerogel dressing.
The inventive method is dissolved in the water around sericin is blended into the gamma-polyglutamic acid-molecule, the sericin side chain contains a large amount of hydrophilic groups, and serine helps immunoglobulin and production of antibodies to wound healing and antibacterial very big facilitation is arranged in the sericin, and the appropriate ratio of 18 seed amino acids is conducive to people's epidermis fibroblast proliferation in the sericin.Therefore, compare with traditional dressing, this aerogel dressing just has high absorbent, excellent biological compatibility, biological degradability and bacteriostasis.And compare with commercially available aerogel dressing, the aerogel dressing that makes by this method has better biocompatibility and bacteriostasis.
By swelling ratio and porosity measurement, this aerogel dressing porosity is up to 98%, has very strong absorbent, so because big porosity, can in this aerogel dressing, add medicine, effectively promote wound healing and anti-inflammation drugs such as YUNNAN BAIYAO, ofloxacin, Mupirocin Ointment etc., make this aerogel dressing have functions such as good blood coagulation, antiinflammatory and promotion wound healing.
Compared with prior art, the invention has the beneficial effects as follows: be reflected at aqueous phase and carry out, be conducive to environmental protection, the response time is short, and the aerogel dressing absorbency is very strong, has excellent biological compatibility, biological degradability and bacteriostasis.
[description of drawings]:
Fig. 1 is the chemical structural formula of gamma-polyglutamic acid-
Fig. 2 is γ-PGA/SS hydrogel preparating mechanism
Fig. 3 (a) is the SEM figure of example 1 hydrogel, (b) is the SEM figure of example 4 hydrogels
[specific embodiment]:
Embodiment 1:
Weighing gamma-polyglutamic acid-1.29g, sericin 2.58g are in there-necked flask, in there-necked flask, add the 7.31g deionized water, after waiting to stir, weighing Ethylene glycol diglycidyl ether 0.87g is in there-necked flask, stir, HCl with 1M regulates initial pH=3～5, after stirring solution is injected homemade mould.60 ℃ of isothermal reaction 5h form gel, and the hydrogel for preparing is soaked to remove not monomer and the cross-linking agent of complete reaction in deionized water, hydrogel is soaked in normal saline reach swelling equilibrium afterwards.Aerogel dressing thickness 1mm.The equilibrium swelling degree of the aerogel dressing that makes in this example in normal saline is 37, and porosity is 98%.The aerogel dressing that makes in this example is dipped in 1640 cell culture medium 24h, is 48h with soaking culture medium culturing logarithmic (log) phase growth L929 l cell, observes and finds that cell grows fine, and has not observed overt toxicity.
Embodiment 2:
Weighing gamma-polyglutamic acid-1.29g, sericin 2.58g are in there-necked flask, in there-necked flask, add the 7.31g deionized water, after waiting to stir, weighing Ethylene glycol diglycidyl ether 1.33g is in there-necked flask, stir, HCl with 1M regulates initial pH=3～5, after stirring solution is injected homemade mould.70 ℃ of isothermal reaction 5h form gel, and the hydrogel for preparing is soaked to remove not monomer and the cross-linking agent of complete reaction in deionized water, hydrogel is soaked in normal saline reach swelling equilibrium afterwards.Aerogel dressing thickness 1mm.The equilibrium swelling degree of the aerogel dressing that makes in this example in normal saline is 26, and porosity is 96%.The aerogel dressing that makes in this example is dipped in 1640 cell culture medium 24h, with soaking culture medium culturing logarithmic (log) phase growth L929 l cell system, the most of cell attachment of 5h, the cell growing way is normal behind the 48h, but not having example 1 growing way good, may be because much more slightly dosage of crosslinking agent causes.
Embodiment 3:
Weighing gamma-polyglutamic acid-1.29g, sericin 2.58g are in there-necked flask, in there-necked flask, add the 7.31g deionized water, after waiting to stir, weighing Ethylene glycol diglycidyl ether 0.87g is in there-necked flask, stir, HCl with 1M regulates initial pH=3～5, after stirring solution is injected homemade mould.40 ℃ of isothermal reaction 5h form gel, and the hydrogel for preparing is soaked to remove not monomer and the cross-linking agent of complete reaction in deionized water, hydrogel is soaked in normal saline reach swelling equilibrium afterwards.Aerogel dressing thickness 1mm.The equilibrium swelling degree of the aerogel dressing that makes in this example in normal saline is 39, and porosity is 98%.The aerogel dressing that makes in this example is dipped in 1640 cell culture medium 24h, is 48h with soaking culture medium culturing logarithmic (log) phase growth L929 l cell, observes and finds that cell grows fine, and has not observed overt toxicity.
Embodiment 4:
Weighing gamma-polyglutamic acid-1.29g, sericin 1.29g are in there-necked flask, in there-necked flask, add the 7.31g deionized water, after waiting to stir, weighing Ethylene glycol diglycidyl ether 0.44g is in there-necked flask, stir, HCl with 1M regulates initial pH=3～5, after stirring solution is injected homemade mould.60 ℃ of isothermal reaction 5h form gel, and the hydrogel for preparing is soaked to remove not monomer and the cross-linking agent of complete reaction in deionized water, hydrogel is soaked in normal saline reach swelling equilibrium afterwards.Aerogel dressing thickness 1mm.The equilibrium swelling degree of the aerogel dressing that makes in this example in normal saline is 35, and porosity is 97%.The aerogel dressing that makes in this example is dipped in 1640 cell culture medium 24h, is 48h with soaking culture medium culturing logarithmic (log) phase growth L929 l cell, observes and finds that cell grows fine, and has not observed overt toxicity.And this aerogel dressing degraded is very fast, and degradation rate is 65% during 6d.
Claims (6)
1. a gamma-polyglutamic acid-/sericin (the aerogel dressing preparation method of γ-PGA/SS), adopt micromolecular cross-linking agent Ethylene glycol diglycidyl ether, in aqueous solution, be prepared, it is characterized in that having introduced the sericin monomer, advantage such as sericin has that excellent biological compatibility, biodegradability and catabolite are nontoxic, environmental protection, good springiness, breathability are good; The method for preparing aerogel dressing comprises: the gamma-polyglutamic acid-with 15%～30% and the sericin of certain mass are dissolved in the deionized water, press the Ethylene glycol diglycidyl ether that 16%～65% of gamma-polyglutamic acid-monomer molar amount adds respective amount, stir under the room temperature, the solution injection is reacted 5h from molding jig in 40～80 ℃ of water-baths, can make gamma-polyglutamic acid-/sericin aerogel dressing.
2. gamma-polyglutamic acid-/sericin according to claim 1 (aerogel dressing of γ-PGA/SS), it is characterized in that: this aerogel dressing comprises: gamma-polyglutamic acid-, sericin, deionized water, Ethylene glycol diglycidyl ether, and the mass percent of each component is:
3. (aerogel dressing of γ-PGA/SS), it is characterized in that: gamma-polyglutamic acid-concentration is 15%～25% aerogel dressing best performance that makes to gamma-polyglutamic acid-/sericin according to claim 1.
4. (aerogel dressing of γ-PGA/SS), it is characterized in that: reaction temperature is 40～60 ℃ of aerogel dressing best performances that make to gamma-polyglutamic acid-/sericin according to claim 1.
5. (aerogel dressing of γ-PGA/SS) is characterized in that gamma-polyglutamic acid-/sericin according to claim 1: the aerogel dressing best performance that makes by 25～32.5% Ethylene glycol diglycidyl ethers that add respective amount of gamma-polyglutamic acid-monomer molar amount.
6. (aerogel dressing of γ-PGA/SS), it is characterized in that: in normal saline, the equilibrium swelling degree is 20～40 to gamma-polyglutamic acid-/sericin according to claim 1.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105079877A (en) * | 2014-05-20 | 2015-11-25 | 中国科学院苏州纳米技术与纳米仿生研究所 | Silk fibroin periodontal patch and preparation method thereof |
| CN105601952A (en) * | 2016-01-14 | 2016-05-25 | 北京化工大学 | Polyaspartic acid composite hydrogel and preparation method thereof |
| CN105920659A (en) * | 2016-05-11 | 2016-09-07 | 温州医科大学 | Bacteriostatic hydrogel dressing for wound repair and preparation method thereof |
| CN108478527A (en) * | 2018-04-19 | 2018-09-04 | 中原工学院 | A kind of Preparation method and use of sericin and gamma-polyglutamic acid self-assembly nano-micelle |
| CN109453410A (en) * | 2018-11-24 | 2019-03-12 | 李小军 | A kind of degradable dressing of animal doctor's antibiotic-free and preparation method thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105079877A (en) * | 2014-05-20 | 2015-11-25 | 中国科学院苏州纳米技术与纳米仿生研究所 | Silk fibroin periodontal patch and preparation method thereof |
| CN105601952A (en) * | 2016-01-14 | 2016-05-25 | 北京化工大学 | Polyaspartic acid composite hydrogel and preparation method thereof |
| CN105601952B (en) * | 2016-01-14 | 2018-07-10 | 北京化工大学 | A kind of poly-aspartate composite hydrogel and preparation method thereof |
| CN105920659A (en) * | 2016-05-11 | 2016-09-07 | 温州医科大学 | Bacteriostatic hydrogel dressing for wound repair and preparation method thereof |
| CN108478527A (en) * | 2018-04-19 | 2018-09-04 | 中原工学院 | A kind of Preparation method and use of sericin and gamma-polyglutamic acid self-assembly nano-micelle |
| CN108478527B (en) * | 2018-04-19 | 2020-05-22 | 中原工学院 | Preparation method and application of sericin and gamma-polyglutamic acid self-assembly nano micelle |
| CN109453410A (en) * | 2018-11-24 | 2019-03-12 | 李小军 | A kind of degradable dressing of animal doctor's antibiotic-free and preparation method thereof |
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Application publication date: 20130918 |