CN117136950B - 一种楚雄腮扁叶蜂引诱剂 - Google Patents
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Abstract
本发明属于病虫害防治技术领域,具体公开一种楚雄腮扁叶蜂引诱剂,该引诱剂具体应用于楚雄腮扁叶蜂防治中,所述引诱剂由α‑石竹烯、木罗烯、毕澄茄烯、β‑杜松烯构成,α‑石竹烯、木罗烯、毕澄茄烯、β‑杜松烯的质量比为0.5~1:1~2:0.5~1:1,本发明获得的植物挥发性物质组合形成的引诱剂,具有活性高、特异性强、使用简便、不污染环境、不伤害天敌等优点,作为楚雄腮扁叶蜂防治措施具有非常明显的优势和益处。
Description
技术领域
本发明属于病虫害防治技术领域,具体涉及一种楚雄腮扁叶蜂引诱剂。
背景技术
楚雄腮扁叶蜂(Cephalcia chuxiongica)隶属膜翅目(Hymenoptera)扁叶蜂科(Pamphiliidae)腮扁叶蜂属(Cephalcia),是近年来云南省新发现的重大食叶害虫,研究显示,云南松及华山松最易遭受楚雄腮扁叶蜂的危害,雪松、滇油杉等其他针叶树种受到的危害较轻,楚雄腮扁叶蜂严重影响西南地区林业产业发展,且该害虫一直呈现扩散蔓延之势,每年都造成很大的损失,昆虫化学信息物质具有活性高、特异性强、使用简便、不污染环境、不伤害天敌等优点,作为害虫防治措施具有非常明显的优势和益处所以利用昆虫化学信息物质这种无毒害的新型环保方法对其进行有效监测和防治意义重大。
植物挥发性物质是指植物在代谢过程中产生的一些短链的碳氢化合物及其衍生物,具有含量微小、成分多样以及易变化等特点。挥发性物质在植物体内分为两大类,一类是一般植物都有的成分,另一类是具有高度特异性的, 植物挥发性物质在昆虫的寄主植物定位、寻找合适产卵场所、取食行为、天敌寻找寄主和作为植物的间接防御手段等方面起重要作用,已有研究显示,寄主松树的针叶粗提物对楚雄腮扁叶蜂雄蜂和抱卵雌蜂具有明显的引诱作用,但是,植物挥发性物质的组成除了因不同种类和品系而不同外,同一植株由于发育阶段不同,部位不同以及昼夜节律的变化,挥发性物质的成分也发生较大变化,而且虫害与机械损伤也能诱导植物散发更多的挥发性物质,故而松树的针叶粗提物成分并不稳定,无法进行广泛的推广使用。
楚雄腮扁叶蜂一般情况下进行有性生殖,在缺乏雄虫时,楚雄腮扁叶蜂雌虫也可进行孤雌生殖,即不经交配所产下的卵也可发育成幼虫,雌性个体产下的卵不经雄性受精就能发育成完整新个体的生殖方式称为孤雌生殖(parthenogenesis),孤雌生殖在昆虫纲特别是膜翅目中有很高的发生比例,昆虫的孤雌生殖能力是造成农林灾害的一个重要而常被忽视的因素。孤雌生殖具有效率高、繁殖速度快等特点,由于楚雄腮扁叶蜂孤雌生殖方式,基于此,研究对楚雄腮扁叶蜂雌性更有吸引力的引诱剂,对害虫进行诱杀,探索新的防治方法十分必要。
昆虫对不同寄主植物偏好性有很大差异。如华北大黑鳃金龟( Holotrichiaoblita) 的偏好寄主为梨树(Pyrus),暗黑鳃金龟的偏好寄主为榆树( Ulmuspumila) 、山楂( Crataegus pinnatifida) 和梨树,研究发现,楚雄腮扁叶蜂雌蜂偏向选择云南松、华山松作为其产卵寄主,而避免在滇油杉(Keteleeria evelyniana)上产卵,且对云南松的危害更加严重。这种选择偏好很大程度与植物挥发物有关。在复杂环境中,植物挥发物在害虫寻找食物、产卵地点和交配等一系列生命活动中发挥着重要作用,昆虫依据寄主所散发的特殊气味物质来辨别寄主,选择合适的产卵场所,以保证下一代能有足够的食料,伍苏然等运用 GC-MS 方法对云南松健康松与衰弱松针叶挥发物化学成分做了比较,结果表明,云南松由健康松转向衰弱松的过程中挥发性成分及含量发生了很大变化,两种状态下针叶挥发性物质中共有15种相异化合物成分,18种相同化合物成分,相同化合物成分在含量上存在很大差异,陈敏等运用 GC-MS 方法分析了云南松健康松针和衰弱松针不同部位挥发性物质的组分及含量变化,结果表明,健康松针中部挥发性物质对雌蜂具有较强的引诱作用,健康松针和衰弱松针不同部位挥发性物质的种类及挥发量存在显著差异,但具体是哪些特殊气味能对雌性楚雄腮扁叶蜂有引诱作用,并未有进一步的研究。
发明内容
本发明的主要目的是提供一种针对楚雄腮扁叶蜂的引诱剂,以解决背景技术中存在的问题。
为实现以上目的,本发明提供以下技术方案:
一种楚雄腮扁叶蜂引诱剂在楚雄腮扁叶蜂防治领域的应用,所述引诱剂由α-石竹烯、木罗烯、毕澄茄烯、β-杜松烯构成,α-石竹烯、木罗烯、毕澄茄烯、β-杜松烯的质量比为0.5~1:1~2:0.5~1:1。
进一步的,使用时,将楚雄腮扁叶蜂引诱剂溶于溶剂中,每毫升溶剂中含有α-石竹烯0.5~1mg、木罗烯1~2 mg、毕澄茄烯0.5~1mg、β-杜松烯1mg。
优选的,每毫升溶剂中含有α-石竹烯0.5mg、木罗烯1mg、毕澄茄烯1mg、β-杜松烯1mg。
优选的,每毫升溶剂中含有α-石竹烯1mg、木罗烯2mg、毕澄茄烯0.5mg、β-杜松烯1mg。
优选的,所述溶剂为无水乙醇。
本发明还提供了一种诱芯应用在楚雄腮扁叶蜂防治领域,诱芯含有上述的楚雄腮扁叶蜂引诱剂。诱芯作为诱捕器结构的一部分,一般由诱芯载体和引诱剂构成,诱芯载体可以是橡皮头、橡胶塞、海绵、毛细管等任何现有技术公开的载体类型。
本发明还提供了一种诱捕器应用在楚雄腮扁叶蜂防治领域,其组成部件中包括所述引诱剂制备的诱芯。诱捕器的类型可以是粘胶式或捕集式等多种类型,粘胶式如三角型诱虫板、昆虫信息素小/大船型诱捕器,捕集式如通用桶型诱捕器、天牛诱捕器等。
本发明达到的技术效果:
本发明获得的植物挥发性物质组合形成的引诱剂,具有活性高、特异性强、使用简便、不污染环境、不伤害天敌等优点,作为楚雄腮扁叶蜂防治措施具有非常明显的优势和益处。
本发明的引诱剂是特别的针对雌性楚雄腮扁叶蜂而进行的研发,对未交配雌性楚雄腮扁叶蜂和已交配雌性楚雄腮扁叶蜂都具有较强的引诱活性,从而进一步的抑制了由楚雄腮扁叶蜂孤雌生殖方式带来的害虫大量繁殖的情况发生,对楚雄腮扁叶蜂的有效监测和防治意义重大。
附图说明
图1不同化合物对未交配雌蜂行为影响统计图(*表示P <0 .05;**表示P <0.01);
图2不同化合物组合引诱剂对未交配雌蜂行为影响统计图(不同标记字母代表差异显著,P <0 .01);
图3不同化合物组合引诱剂对已交配雌蜂行为影响统计图(不同标记字母代表差异显著,P <0 .01);
图4 林间平均诱捕量比较统计图(*表示P <0 .05;**表示P <0 .01);
图5 林间诱捕效果图(A为对照组,B为处理组)。
具体实施方式
除非特别指明,否则基本上按照本领域内熟知的以及在各种参考文献中描述的常规方法进行实施例中描述的实验和方法。实施例中未注明具体条件者,按照常规条件或制造商建议的条件进行。所用试剂或仪器未注明生产厂商者,均为可以通过市购获得的常规产品。
实施例1
一种应用于楚雄腮扁叶蜂防治的引诱剂,由α-石竹烯、木罗烯、毕澄茄烯、β-杜松烯构成,α-石竹烯、木罗烯、毕澄茄烯、β-杜松烯的质量比为0.5:1:1:1。
实施例2
一种应用于楚雄腮扁叶蜂防治的引诱剂,由α-石竹烯、木罗烯、毕澄茄烯、β-杜松烯构成,α-石竹烯、木罗烯、毕澄茄烯、β-杜松烯的质量比为1:2:0.5:1。
实施例3
一种应用于楚雄腮扁叶蜂防治的引诱剂,溶剂为无水乙醇,每毫升溶剂中含有α-石竹烯0.5mg、木罗烯1mg、毕澄茄烯1mg、β-杜松烯1mg。
实施例4
一种应用于楚雄腮扁叶蜂防治的引诱剂,溶剂为无水乙醇,每毫升溶剂中含有α-石竹烯1mg、木罗烯2mg、毕澄茄烯0.5mg、β-杜松烯1mg。
为了进一步说明,本发明提供的实施方案中引诱剂效果的可行性,下面结合具体的试验进行说明。
1、试验材料
1.1化合物
在现有研究的基础上,综合考虑云南松健康松针与衰弱松针不同挥发性物质的组分及含量变化,针对健康松针和衰弱松针中挥发物种类及挥发量存在的显著差异,选择如表1所示的8种化合物,测试其对雌性楚雄腮扁叶蜂的引诱活性。
表1 标准化合物纯度和来源
1.2试验用虫
供试昆虫采自云南省昆明市云南松林地内,收集经过越冬即将羽化的楚雄腮扁叶蜂蛹或者已经羽化尚未出土的成虫,带回室内。将蛹放入透明塑料圆盒内,覆盖湿润的原土待其羽化。采集的成虫辨别雌雄蜂,放入底部用滤纸保湿装有新鲜云南松松梢的养蜂笼内,定期添加新鲜松针保证充足的食物来源,饲养备用,养蜂笼温度为 20~28℃,湿度(60±10)%,试验前两天,让部分雌蜂和雄蜂交配,获得已交配雌蜂和未交配雌蜂,并取出食物,间隔3小时后进行试验。
2、试验方法
2.1 室内选择试验
室内趋向试验参考:冯丹,王艺璇,管国伟等.伴生菌诱导的云南松挥发性化合物对云南切梢小蠹嗅觉行为影响研究[J].西部林业科学,2021,50(03):151-156,使用多臂嗅觉仪,将不同的化合物用无水乙醇稀释成浓度 1mg/mL备用,分别在2cm×4 cm 的灭菌滤纸上加入200uL化合物,对照为等计量的无水乙醇,然后将滤纸放到多臂嗅觉仪的各个气味瓶中,进气流量控制在250mL/min,室内温度控制在 25℃左右,测试期间用黑布遮光。采用饥饿雌蜂300头备用。每次将30头雌蜂放入多臂嗅觉仪的接虫室后计时,10 min 后记录各气味瓶中雌蜂的数量。重复10次,每次测试后顺时针更换各气味瓶的位置,并更换滤纸。试验结束后用95%乙醇冲洗整个装置待用。
选择率(%)= Σ各气味瓶中的蜂数/测试总蜂数×100
校正选择率(%)=(处理组选择率对照组选择率)/对照组选择率×100
2.2林间引诱试验
试验地位于云南省昆明市寻甸回族彝族自治县(25°18′N,103°12′E)楚雄腮扁叶蜂发生较重的区域,试验地为云南松纯林,林组为成熟林,平均树龄为20年以上,郁闭度0.6,土壤以红壤为主,土层厚度约 40cm,平均海拔1970m,气候属于典型的北亚热带季风气候,年平均气温14.9℃。
林间试验用诱捕器采用市场购买的黄色粘虫板(23*10cm)。每个粘虫板上喷引诱剂1ml,即为诱捕装置。将诱捕装置悬挂于云南松约 2.5~3.0 m 高度的侧枝上,诱捕装置之间间距最小30m,设10个重复,以未带诱芯的诱捕器为对照,8d 后检查诱捕情况。
3、结果与分析
3.1 室内选择行为
室内选择行为试验结果见图1~3,由图1,未交配雌性楚雄腮扁叶蜂对8种化合物中的α-石竹烯、木罗烯、毕澄茄烯、β-杜松烯的引诱活性最强,表现出明显的趋向反应,未交配雌性楚雄腮扁叶蜂选择率分别是:18%、16%、15%、22%,萜品油烯、β-榄香烯、对苯二甲酸二辛酯、杜松烯对未交配雌性楚雄腮扁叶蜂引诱活性相对较差,特别是β-榄香烯引诱活性最差。
根据未交配雌蜂对8种化合物的引诱活性反应,设计如表2所示的正交实验组合,进一步测试不同组合对未交配雌蜂和已经交配雌蜂的引诱活性,由图2可以看出,T2、T6组合对未交配雌性楚雄腮扁叶蜂的引诱活性最强,选择率分别是:25%、28%,表现出极显著的趋向性,其次是T3、T5组合对未交配雌性楚雄腮扁叶蜂表现出相对显著的趋向性,选择率分别是:10%、11.5%,最后是T1、T4、T7、T8、T9组合对未交配雌性楚雄腮扁叶蜂引诱活性最差。
由图3可以看出,不同组合对未交配的雌性楚雄腮扁叶蜂和已经交配的雌性楚雄腮扁叶蜂展现出的活性有所不同,T2组合对已交配雌性楚雄腮扁叶蜂的引诱活性最强,趋向性最明显,选择率是21%,T6组合也展现出相对较强的引诱活性,但是对比未交配的雌性楚雄腮扁叶蜂引诱活性相对降低,选择率是15%,T3、T5、T7、T8、T9组合都相对展现出了一定的引诱活性,以T1、T4组合对已经交配的雌性楚雄腮扁叶蜂引诱活性最差。
表2不同引诱剂组合设计表
3.2林间引诱分析
由图2、图3的统计数据可以看出,不同浓度搭配组合下,对已交配雌蜂和未交配雌蜂的引诱活性有所不同,基于最优数据选择,选择T2的搭配组合,以无水乙醇为溶剂,配置含量为α-石竹烯0.5mg/mL、木罗烯1mg/m L、毕澄茄烯1mg/m L、β-杜松烯1mg /m L组合的引诱剂,进一步的做林间试验。
由图4、图5可以看出,与对照相比,添加引诱剂的诱捕器,对雌蜂显示出了极显著的引诱活性,其中,对未交配的雌性楚雄腮扁叶蜂引诱活性最强,其次是已经交配的雌性楚雄腮扁叶蜂,同时,林间试验显示,对雄性楚雄腮扁叶蜂也表现了一定的引诱活性,但是引诱活性相对雌性并不显著。
影响昆虫选择寄主植物的因素很多,如植物化学成分、植物形态特征、各种环境因子光、温度、湿度等和其它生物等,但是,植物的化学成分即植物挥发性物质在植食性昆虫的寄主选择中起着最重要的作用,植食性昆虫利用寄主植物所释放的化学信号来确定自己的飞行行为,从而准确地找到寄主植物,几乎所有种类的昆虫都利用寄主散发的化学物质来发现适合于自己的寄主,如果没有植物气味的存在,多数植食性昆虫找到寄主植物的概率非常低,将直接影响这些种类昆虫的生存繁殖。
基于前期已有研究对云南松健康松针与衰弱松针成分的分析对比,本研究选择了8种挥发性成分,探索了未交配和已交配雌性楚雄腮扁叶蜂对这些挥发性成分的趋向行为反应,结果表明雌性楚雄腮扁叶蜂对8种化合物有不同程度的响应,对α-石竹烯、木罗烯、毕澄茄烯、β-杜松烯四种化合物显示出较强的引诱活性,在生产实践中,单一植物挥发物往往无法达到理想的诱虫效果,将不同挥发物按一定比例混合使用可增强对昆虫的引诱作用。本研究进一步的设计了不同组合的化合物配比,结果表明,α-石竹烯0.5mg/ml、木罗烯1mg/ml、毕澄茄烯1mg/ml、β-杜松烯1mg/ml的组合配比下,引诱效果最好,对未交配和已经交配的雌性楚雄腮扁叶蜂都具有较好的引诱效果,且林间试验显示,该组合下,对雄性楚雄腮扁叶蜂也具有一定的引诱活性,对雄性楚雄腮扁叶蜂的引诱活性,可能是基于引诱剂本身的效果,也可能是未交配或已经交配的雌性楚雄腮扁叶蜂散发的性信息素的影响,需要进一步的研究。
本发明旨在涵盖落入所附权利要求的宽泛范围之内的所有这样的替换、修改和变型。因此,凡在本发明的精神和原则之内,所做的任何省略、修改、等同替换、改进等,均应包含在本发明的保护范围之内。
Claims (6)
1.一种楚雄腮扁叶蜂引诱剂在楚雄腮扁叶蜂防治领域的应用,其特征在于,所述引诱剂由α-石竹烯、木罗烯、毕澄茄烯、β-杜松烯构成,α-石竹烯、木罗烯、毕澄茄烯、β-杜松烯的质量比为0.5~1:1~2:0.5~1:1。
2.根据权利要求1所述的应用,其特征在于,将所述楚雄腮扁叶蜂引诱剂溶于溶剂中,每毫升溶剂中含有α-石竹烯0.5~1mg、木罗烯1~2 mg、毕澄茄烯0.5~1mg、β-杜松烯1mg。
3.根据权利要求2所述的应用,其特征在于,每毫升溶剂中含有α-石竹烯0.5mg、木罗烯1mg、毕澄茄烯1mg、β-杜松烯1mg。
4.根据权利要求2所述的应用,其特征在于,每毫升溶剂中含有α-石竹烯1mg、木罗烯2mg、毕澄茄烯0.5mg、β-杜松烯1mg。
5.根据权利要求2所述的应用,其特征在于,所述溶剂为无水乙醇。
6. 如权利要求1-5 任一所述应用中使用的引诱剂制备的诱芯或诱捕器在楚雄腮扁叶蜂防治领域的应用。
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