CN116941620A - 具有防治苹果根茎腐病功能的组合物及其应用 - Google Patents
具有防治苹果根茎腐病功能的组合物及其应用 Download PDFInfo
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- CN116941620A CN116941620A CN202310734746.4A CN202310734746A CN116941620A CN 116941620 A CN116941620 A CN 116941620A CN 202310734746 A CN202310734746 A CN 202310734746A CN 116941620 A CN116941620 A CN 116941620A
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- DHRLEVQXOMLTIM-UHFFFAOYSA-N phosphoric acid;trioxomolybdenum Chemical compound O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.OP(O)(O)=O DHRLEVQXOMLTIM-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds containing nitrogen-to-oxygen bonds
- A01N33/18—Nitro compounds
- A01N33/20—Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group
- A01N33/22—Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group having at least one oxygen or sulfur atom and at least one nitro group directly attached to the same aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/02—Other organic fertilisers from peat, brown coal, and similar vegetable deposits
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Engineering & Computer Science (AREA)
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- General Health & Medical Sciences (AREA)
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Inorganic Chemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
本发明涉及防治苹果根茎腐病领域,公开了一种具有防治苹果根茎腐病功能的组合物及其应用。该组合物包括:A组分:吲丁·诱抗剂、复硝酚钠和氯溴异氰尿酸;B组分:芽孢杆菌菌剂和含腐殖酸水溶肥料;其中,A组分和含腐殖酸水溶肥料的干重重量比为0.15‑0.3:1。本发明提供的组合物既可以提供苹果生长所需要的营养,又可以防治苹果根茎腐病害,同时也能够增强苹果抗逆性、提高产量,对苹果增产控病具有良好的效果。
Description
技术领域
本发明涉及防治苹果根茎腐病领域,具体涉及一种具有防治苹果根茎腐病功能的组合物及其应用。
背景技术
苹果为我国主要栽培水果种类。苹果产业在我国农业生产中占有重要地位。苹果根茎腐病害是引起苹果根部或茎部侵害而引起的病害,是苹果中普遍发生且危害严重的侵染性病害。近年来由于气候、耕作制度和农业产业结构的调整,苹果根茎腐病害对苹果的危害日益严重,苹果产区一旦发生,严重影响苹果的产量和质量,并且苹果根茎腐病害随土壤、雨水、病残体残留在土壤中很彻底难清除。然而,现有技术中,对苹果根茎腐病害的防治方法比较单一,如单一使用大量化学药剂进行防治,使用剧毒化学药剂进行土壤熏蒸等,忽略了土壤有益微生物的作用以及植株本身的营养需求与抗性培养,并且大量单一化学药的使用使得有害生物抗性增强,难以防治,因此,生物农药与化学农药合理搭配使用技术急需提高。亟待开发一种生物农药化学农药合理混配使用技术,增强苹果抗性、提高苹果产量并且能够有效防控苹果根茎腐病害的组合产品,实现苹果产业可持续发展。
发明内容
本发明的目的是为了克服现有技术存在的问题,提供一种具有防治苹果根茎腐病功能的组合物及其应用。
为了实现上述目的,本发明第一方面提供了一种具有防治苹果根茎腐病功能的组合物,该组合物包括:
A组分:吲丁·诱抗剂、复硝酚钠和氯溴异氰尿酸;
B组分:芽孢杆菌菌剂和含腐殖酸水溶肥料;
其中,A组分和含腐殖酸水溶肥料的干重重量比为0.15-0.3:1。
本发明第二方面提供了一种防治苹果根茎腐病的方法,所述方法包括:将上述的组合物施用于苹果植株。
本发明第三方面提供了上述的组合物在苹果增产和/或防治苹果根茎腐病中的应用。
通过上述技术方案,本发明提供的组合物既可以提供苹果生长所需要的营养,又可以防治苹果根茎腐病害,同时也能够增强苹果抗逆性、提高产量,对苹果增产控病具有良好的效果。
具体实施方式
在本文中所披露的范围的端点和任何值都不限于该精确的范围或值,这些范围或值应当理解为包含接近这些范围或值的值。对于数值范围来说,各个范围的端点值之间、各个范围的端点值和单独的点值之间,以及单独的点值之间可以彼此组合而得到一个或多个新的数值范围,这些数值范围应被视为在本文中具体公开。
未作相反说明的情况下,所有物质的重量以其干重计。
本发明第一方面提供了一种具有防治苹果根茎腐病功能的组合物,该组合物包括:
A组分:吲丁·诱抗剂、复硝酚钠和氯溴异氰尿酸;
B组分:芽孢杆菌菌剂和含腐殖酸水溶肥料;
其中,A组分和含腐殖酸水溶肥料的干重重量比为0.15-0.3:1,可以为0.15:1、0.16:1、0.17:1、0.18:1、0.19:1、0.2:1、0.21:1、0.22:1、0.23:1、0.24:1、0.25:1、0.26:1、0.27:1、0.28:1、0.29:1、3:1或以上任意两数值形成的范围及范围内的值。
本发明中,为了使得苹果防治苹果根茎腐病害和增加产量的效果更好,所述吲丁·诱抗剂和复硝酚钠的重量比为1:0.9-7.5,可以为1:0.9、1:1、1:1.5、1:2、1:2.5、1:3、1:3.5、1:4、1:4.5、1:5、1:5.5、1:6、1:6.5、1:7、1:7.5或以上任意两数值形成的范围及范围内的值,优选为1:1.5-5.5。
本发明中,为了使得苹果防治苹果根茎腐病害和增加产量的效果更好,所述吲丁·诱抗剂和氯溴异氰尿酸的重量比为1:25-200,可以为1:25、1:30、1:40、1:50、1:60、1:70、1:80、1:90、1:100、1:110、1:120、1:130、1:140、1:150、1:160、1:170、1:180、1:190、1:200或以上任意两数值形成的范围及范围内的值,优选为1:50-150。
本发明中,为了使得苹果防治苹果根茎腐病害和增加产量的效果更好,相对于1g所述含腐殖酸水溶肥料干重,所述菌剂中的活菌数为109-1011CFU。
本发明中,所述吲丁·诱抗剂由吲哚丁酸与S-诱抗素复配而成,吲丁·诱抗剂中S-诱抗素和吲哚丁酸的重量比为1:8-12。
优选的,所述复硝酚钠包括5-硝基愈创木酚钠、邻硝基苯酚钠和对硝基苯酚钠,更优选的,所述5-硝基愈创木酚钠与邻硝基苯酚钠的重量比为1:0.2-2,所述5-硝基愈创木酚钠与对硝基苯酚钠的重量比为1:1.2-3。
本发明中,为了使得苹果防治苹果根茎腐病害和增加产量的效果更好,所述芽孢杆菌菌剂中包括枯草芽孢杆菌、地衣芽孢杆菌和多粘类芽孢杆菌中的至少一种,优选为枯草芽孢杆菌、地衣芽孢杆菌和多粘类芽孢杆菌,活菌数比例为1:0.5-2:0.5-2。所述枯草芽孢杆菌优选为保藏号为ACCC 19742的菌株,所述地衣芽孢杆菌优选为保藏号为ACCC 19744的菌株,所述多粘类芽孢杆菌优选为保藏号为ACCC 03605的菌株。
本发明中,为了使得苹果防治苹果根茎腐病害和增加产量的效果更好,本发明使用含腐殖酸水溶肥料,所述含腐殖酸水溶肥料通过在腐殖酸中加入氮元素、磷元素、钾元素、硼元素、锌元素、钼元素得到,所述氮元素、磷元素、钾元素、硼元素、锌元素、钼元素和腐殖酸重量比为1:1-2:1-2:0.1-0.5:0.1-0.5:0.1-0.5:3-5,优选为1:1-1.5:1-1.5:0.1-0.3:0.1-0.3:0.1-0.3:3-4,所述磷元素以P2O5计,所述钾元素以K2O计。
本发明中,在实际使用中,有些商购的试剂往往是已经稀释到一定浓度的,本发明的“干重”是指其中的溶质的重量。
本发明第二方面提供了一种防治苹果根茎腐病的方法,所述方法包括:将上述的组合物施用于苹果植株。
本发明中,为了使得苹果防治苹果根茎腐病害和增加产量的效果更好,所述方法包括:先将A组分施用于苹果植株,再将B组分施用于苹果植株,优选的,A组分与B组分的施用时间间隔为5-7天。所述A组分和B组分在施用前进行稀释;更优选的,所述A组分的稀释液中A组分含量为0.1-0.5g/L,可以为0.1g/L、0.15g/L、0.2g/L、0.25g/L、0.3g/L、0.35g/L、0.4g/L、0.45g/L、0.5g/L或以上任意两数值形成的范围及范围内的值;进一步优选的,所述B组分的稀释液中含腐殖酸水溶肥料的干重含量为0.1-0.7g/L,可以为0.1g/L、0.15g/L、0.2g/L、0.25g/L、0.3g/L、0.35g/L、0.4g/L、0.45g/L、0.5g/L、0.55g/L、0.6g/L、0.65g/L、0.7g/L或以上任意两数值形成的范围及范围内的值。
优选的,所述A组分的施用量为0.1-1.5g/株,可以为0.1g/株、0.15g/株、0.2g/株、0.25g/株、0.3g/株、0.35g/株、0.4g/株、0.45g/株、0.5g/株或以上任意两数值形成的范围及范围内的值,所述施用量可以分2-3次施用,每次间隔4-7天,B组分可以分3-5次施用,每次间隔4-7天。
本发明中,为使得本申请的组合物能更好的发挥效用,所述施用部位为植株根部。
本发明第三方面提供了上述的组合物在苹果增产和/或防治苹果根茎腐病中的应用。
本发明中,所述苹果根茎腐病为由病原菌侵染苹果树根部和/或茎基部引起的根茎腐烂病。优选的,所述病原菌为真菌,如小蜜环菌(Armillariella mellea)、褐座坚壳菌(Rosellinia necatrix)、桑卷担菌(Helicobasidium mompa)、紫卷担菌(Helicobasidiumpurpureum)、齐整小核菌(Sclerotium rolfsii)、尖镰孢菌(Fusarium oxysporium)、腐皮镰孢菌(Fusarium solani)、弯角镰孢菌(Fusarium camptoceras)或立枯丝核菌(Rhizoctonia solani)。更优选的,所述苹果根茎腐病包括苹果根朽病、白纹羽病、紫纹羽病、白绢病、圆斑根腐病和立枯病中的至少一种。
以下将通过实施例对本发明进行详细描述。以下实施例中,含腐殖酸水溶肥料、1重量%吲丁·诱抗剂药剂(S-诱抗素与吲哚丁酸与重量比为1:9)、1.8重量%复硝酚钠药剂(16重量%5-硝基愈创木酚钠、34重量%邻硝基苯酚钠和50重量%对硝基苯酚钠)、50重量%氯溴异氰尿酸药剂均购自河南逸农鸿扬生物科技有限公司。
复合芽孢杆菌菌剂为枯草芽孢杆菌(活菌数为1×109CFU/g,保藏号为ACCC19742)、地衣芽孢杆菌(活菌数为1×109CFU/g,保藏号为ACCC19744)和多粘类芽孢杆菌(活菌数为1×109CFU/g,保藏号为ACCC 03605)均购买自中国农业微生物菌种保藏管理中心。
肥料中的腐植酸含量采用酸沉淀后氧化还原滴定法测定,全氮含量采用凯氏定氮法测定,磷采用磷钼酸喹啉重量法测定,钾用四苯硼酸钾重量法测定,pH采用电位法测定,硼采用甲亚胺比色法测定;肥料中的锌、钼采用火焰原子吸收分光光度法测定;其中含腐殖酸水溶肥料的主要成分如表1所示(其中,水含量为以物料总质量为基准计算,其余成分为以物料中干物质为基准计算)。
表1
表2
实施例与对比例
由以下表3各重量份的试剂配制得到各实施例与对比例的组合物。
表3
实施例12
按照实施例1的方法,不同的是,将枯草芽孢杆菌更换成暹罗芽孢杆菌。暹罗芽孢杆菌菌株保藏号为CCTCC NO:M 2022925(公开号为CN115975855A)。
实施例13
按照实施例1的方法,不同的是,将地衣芽孢杆菌更换成卡式芽孢杆菌。卡式芽孢杆菌菌株保藏号为CCTCC NO:M 20221660(公开号为CN116254197A)。
实施例14
按照实施例1的方法,不同的是,将多粘类芽孢杆菌更换成短小芽孢杆菌。拟蕈状芽孢杆菌菌株保藏号CCTCC NO:M 2022924(公开号为CN115851502A)。
对比例3
按照实施例1的方法,不同的是,将其中的含腐殖酸水溶肥料替换为等重量份(以干物质计)的氨基酸类水溶肥,氨基酸类水溶肥购自四川沃梵生物科技有限责任公司,主要成分见表2。
对比例4
按照实施例1的方法,不同的是,将其中的复合芽孢杆菌菌剂替换为等活菌数的绿色木霉,绿色木霉购自水谷欣生物科技有限公司。
对比例5
按照实施例1的方法,不同的是,将其中的吲丁·诱抗剂替换为等重量份(以干物质计)的单剂的吲哚乙酸。
对比例6
按照实施例1的方法,不同的是,将其中的复硝酚钠替换为等重量份(以干物质计)的14-羟基天然芸苔素,的14-羟基天然芸苔素购自潍坊奥丰作物病害防治有限公司。
对比例7
按照实施例1的方法,将氯溴异氰尿酸换为等重量份(以干物质计)的春雷霉素,春雷霉素购自山东瑞星生物有限公司。
对比例8
按照实施例1的方法,不同的是,不加入复合芽孢杆菌菌剂。
对比例9
按照实施例1的方法,不同的是,不加入含腐殖酸水溶肥料。
测试例1
在田间出现叶片萎蔫树势弱的情况下,田间观察到轻微萎蔫后将根部刨开,进行观察发病达一级感染,通过鉴定病原菌来确定是哪种病。根和茎的少量表层组织受到感染,表现为轻微的褐色病斑情况下,刮除病部,并按上述实施例及对比例得到含A组分和含B组分别与水混合,A组分稀释液中A组分含量为0.3g/L,B组分稀释液中含腐殖酸水溶肥料的干重含量为0.3g/L。先根际施用A组分2次,两次施用间隔5天,5天后施用B组分于苹果植株根际,B组分使用频率4次,每次间隔6天。同时设置未施用该配方制剂(对照组)的患有苹果根茎腐病的植株(每个试验组选择发病达一级感染的病株20株挂牌记录进行实验,53株/亩)和未患有苹果根茎腐病的植株(正常组)。在上一年7月份苹果采收之后,在上一年9月份施加基肥(有机肥3500kg/亩,磷酸二铵21.7kg/亩,尿素9.1kg/亩,其中,有机肥猪粪发酵后的农家肥,N含量2重量%),施基肥深度为50-60公分,开沟施入,浅施覆土。2月份施加本发明的组合物,之后每个月调查发病情况,至采收结束。随滴灌***施入追肥:3月施加第一次追肥(尿素11kg/亩),并在叶面喷洒0.4重量%尿素液100g/亩,5月份施加第二次追肥(尿素9.1kg/亩,硫酸钾16kg/亩),并在叶面喷洒0.4重量%尿素液100g/亩,7月份施加第三次追肥(尿素11kg/亩,硫酸钾12kg/亩),并在叶面喷洒0.4重量%磷酸二氢钾100g/亩,8月份进行苹果采收,计算病害防控效果如表4所示,苹果产量如表5所示。
根据我国农业部发布的《果树病害诊断技术规范》中,对苹果根茎腐烂病的病害分级标准如下:
一级感染:根和茎的少量表层组织受到感染,表现为轻微的褐色病斑。
二级感染:根和茎部分组织已经受到感染,表现为局部的茎部皮层和木质部出现褐色至黑色的腐烂病斑,而茎部仍能保持正常形态。
三级感染:根的大部分组织和茎的表层和深层组织均已受到严重感染,表现为部分环状腐烂并有明显的坏死,严重损害树木的生长和形态。
防治效果的计算采用下列两公式:
病情指数=[(Σ(病级株数×代表级数))/(总株数×最高代表级值)]×100%;
防治效果(%)=[(对照组病情指数-处理组病情指数)/对照组病情指数]×100%。
表4
表5
生物制剂编号 | 亩产量(kg/亩) |
实施例1-1 | 5023 |
实施例1-2 | 4898 |
实施例1-3 | 4623 |
实施例2 | 4031 |
实施例3 | 4092 |
实施例4 | 3405 |
实施例5 | 3023 |
实施例6 | 3690 |
实施例7 | 3836 |
实施例8 | 4382 |
实施例9 | 4596 |
实施例10 | 3709 |
实施例11 | 3328 |
实施例12 | 2987 |
实施例13 | 2995 |
实施例14 | 2931 |
对比例1 | 2479 |
对比例2 | 2681 |
对比例3 | 2303 |
对比例4 | 2141 |
对比例5 | 2086 |
对比例6 | 1887 |
对比例7 | 1569 |
对比例8 | 2686 |
对比例9 | 1032 |
对照组 | 863 |
正常组 | 2409 |
通过以上结果可以看出,采用本发明提供的组合物可以更好的防治苹果根茎腐病和明显提高苹果产量效果。
以上详细描述了本发明的优选实施方式,但是,本发明并不限于此。在本发明的技术构思范围内,可以对本发明的技术方案进行多种简单变型,包括各个技术特征以任何其它的合适方式进行组合,这些简单变型和组合同样应当视为本发明所公开的内容,均属于本发明的保护范围。
Claims (10)
1.一种具有防治苹果根茎腐病功能的组合物,其特征在于,该组合物包括:
A组分:吲丁·诱抗剂、复硝酚钠和氯溴异氰尿酸;
B组分:芽孢杆菌菌剂和含腐殖酸水溶肥料;
其中,A组分和含腐殖酸水溶肥料的干重重量比为0.15-0.3:1。
2.根据权利要求1所述的组合物,其中,吲丁·诱抗剂、复硝酚钠和氯溴异氰尿酸的重量比为1:0.9-7.5:25-200。
3.根据权利要求1或2所述的组合物,其中,相对于1g所述含腐殖酸水溶肥料干重,所述芽孢杆菌菌剂中的活菌数为109-1011CFU。
4.根据权利要求1或2所述的组合物,其中,所述吲丁·诱抗剂中S-诱抗素和吲哚丁酸的重量比为1:8-12;
和/或,所述复硝酚钠包括5-硝基愈创木酚钠、邻硝基苯酚钠和对硝基苯酚钠;
优选的,所述5-硝基愈创木酚钠、邻硝基苯酚钠和对硝基苯酚钠的重量比为1:0.2-2:1.2-3。
5.根据权利要求1或2所述的组合物,其中,所述芽孢杆菌菌剂中的微生物包括枯草芽孢杆菌、地衣芽孢杆菌和多粘类芽孢杆菌中的至少一种;
优选的,所述芽孢杆菌菌剂中的微生物包括枯草芽孢杆菌、地衣芽孢杆菌和多粘类芽孢杆菌,活菌数比例为1:0.5-2:0.5-2。
6.根据权利要求1或2所述的组合物,其中,所述含腐殖酸水溶肥料通过在腐殖酸中加入氮元素、磷元素、钾元素、硼元素、锌元素、钼元素得到,所述氮元素、磷元素、钾元素、硼元素、锌元素、钼元素和腐殖酸重量比为1:1-2:1-2:0.1-0.5:0.1-0.5:0.1-0.5:3-5,优选为1:1-1.5:1-1.5:0.1-0.3:0.1-0.3:0.1-0.3:3-4,所述磷元素以P2O5计,所述钾元素以K2O计。
7.一种防治苹果根茎腐病的方法,其特征在于,所述方法包括:将权利要求1-6中任意一项所述的组合物施用于苹果植株。
8.根据权利要求7所述的方法,其中,所述方法包括:先将A组分施用于苹果植株,再将B组分施用于苹果植株;
和/或,所述A组分的施用量为0.1-1.5g/株。
9.根据权利要求8所述的方法,其中,A组分与B组分的施用时间间隔为5-7天;
和/或,所述A组分和B组分在施用前进行稀释;
优选的,所述A组分的稀释液中A组分的含量为0.1-0.5g/L;
优选的,所述B组分的稀释液中含腐殖酸水溶肥料的干重含量为0.1-0.7g/L。
10.权利要求1-7中任意一项所述的组合物在苹果增产和/或防治苹果根茎腐病中的应用;
优选的,所述苹果根茎腐病为由病原菌侵染苹果树根部和/或茎基部引起的根茎腐烂病;
优选的,所述苹果根茎腐病包括苹果根朽病、白纹羽病、紫纹羽病、白绢病、圆斑根腐病和立枯病中的至少一种。
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