CN116813419A - Water-soluble fertilizer and preparation method and application thereof - Google Patents
Water-soluble fertilizer and preparation method and application thereof Download PDFInfo
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- CN116813419A CN116813419A CN202310794751.4A CN202310794751A CN116813419A CN 116813419 A CN116813419 A CN 116813419A CN 202310794751 A CN202310794751 A CN 202310794751A CN 116813419 A CN116813419 A CN 116813419A
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- Prior art keywords
- water
- soluble fertilizer
- potassium
- fertilizer
- protein composition
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 97
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- 101710116034 Immunity protein Proteins 0.000 claims abstract description 45
- 239000000203 mixture Substances 0.000 claims abstract description 36
- 239000004021 humic acid Substances 0.000 claims abstract description 21
- 235000015097 nutrients Nutrition 0.000 claims abstract description 21
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000007921 spray Substances 0.000 claims abstract description 13
- 238000003973 irrigation Methods 0.000 claims abstract description 12
- 230000002262 irrigation Effects 0.000 claims abstract description 12
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 10
- 239000000843 powder Substances 0.000 claims abstract description 8
- 238000003756 stirring Methods 0.000 claims abstract description 6
- 238000002156 mixing Methods 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000008187 granular material Substances 0.000 claims abstract description 4
- 238000002791 soaking Methods 0.000 claims abstract description 4
- 241000196324 Embryophyta Species 0.000 claims description 62
- 239000007788 liquid Substances 0.000 claims description 23
- 235000021307 Triticum Nutrition 0.000 claims description 21
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 18
- 239000011591 potassium Substances 0.000 claims description 18
- 229910052700 potassium Inorganic materials 0.000 claims description 18
- 238000000855 fermentation Methods 0.000 claims description 15
- 230000004151 fermentation Effects 0.000 claims description 15
- 239000000126 substance Substances 0.000 claims description 15
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 claims description 14
- 240000008042 Zea mays Species 0.000 claims description 13
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 12
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 11
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 11
- 235000005822 corn Nutrition 0.000 claims description 11
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 9
- 240000003768 Solanum lycopersicum Species 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 9
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 8
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 8
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 8
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 8
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 8
- 239000004202 carbamide Substances 0.000 claims description 8
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 8
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 8
- 239000011734 sodium Substances 0.000 claims description 8
- 229910052708 sodium Inorganic materials 0.000 claims description 8
- 238000001914 filtration Methods 0.000 claims description 7
- 238000010438 heat treatment Methods 0.000 claims description 7
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 6
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 claims description 6
- 229910000387 ammonium dihydrogen phosphate Inorganic materials 0.000 claims description 6
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 claims description 6
- 235000019837 monoammonium phosphate Nutrition 0.000 claims description 6
- 239000006012 monoammonium phosphate Substances 0.000 claims description 6
- 239000004323 potassium nitrate Substances 0.000 claims description 6
- 235000010333 potassium nitrate Nutrition 0.000 claims description 6
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 6
- ONDPHDOFVYQSGI-UHFFFAOYSA-N zinc nitrate Chemical compound [Zn+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ONDPHDOFVYQSGI-UHFFFAOYSA-N 0.000 claims description 6
- 241000123650 Botrytis cinerea Species 0.000 claims description 5
- 239000011609 ammonium molybdate Substances 0.000 claims description 5
- 235000018660 ammonium molybdate Nutrition 0.000 claims description 5
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 claims description 5
- 229940010552 ammonium molybdate Drugs 0.000 claims description 5
- 239000000084 colloidal system Substances 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 5
- 239000000618 nitrogen fertilizer Substances 0.000 claims description 5
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 5
- 229940118149 zinc sulfate monohydrate Drugs 0.000 claims description 5
- RNZCSKGULNFAMC-UHFFFAOYSA-L zinc;hydrogen sulfate;hydroxide Chemical compound O.[Zn+2].[O-]S([O-])(=O)=O RNZCSKGULNFAMC-UHFFFAOYSA-L 0.000 claims description 5
- 239000004114 Ammonium polyphosphate Substances 0.000 claims description 4
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 4
- 235000019826 ammonium polyphosphate Nutrition 0.000 claims description 4
- 229920001276 ammonium polyphosphate Polymers 0.000 claims description 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 4
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 4
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 4
- CSGLCWIAEFNDIL-UHFFFAOYSA-O azanium;urea;nitrate Chemical compound [NH4+].NC(N)=O.[O-][N+]([O-])=O CSGLCWIAEFNDIL-UHFFFAOYSA-O 0.000 claims description 4
- 239000011575 calcium Substances 0.000 claims description 4
- 239000010949 copper Substances 0.000 claims description 4
- 244000037666 field crops Species 0.000 claims description 4
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 4
- 235000011147 magnesium chloride Nutrition 0.000 claims description 4
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 4
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 4
- 239000011572 manganese Substances 0.000 claims description 4
- 239000002686 phosphate fertilizer Substances 0.000 claims description 4
- 229940072033 potash Drugs 0.000 claims description 4
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims description 4
- 235000015320 potassium carbonate Nutrition 0.000 claims description 4
- 239000001103 potassium chloride Substances 0.000 claims description 4
- 235000011164 potassium chloride Nutrition 0.000 claims description 4
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims description 4
- 229910052939 potassium sulfate Inorganic materials 0.000 claims description 4
- 235000011151 potassium sulphates Nutrition 0.000 claims description 4
- LEZAYTDLNNEFJT-UHFFFAOYSA-N tetracosasodium octaborate tetrahydrate Chemical compound O.O.O.O.[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[O-]B([O-])[O-].[O-]B([O-])[O-].[O-]B([O-])[O-].[O-]B([O-])[O-].[O-]B([O-])[O-].[O-]B([O-])[O-].[O-]B([O-])[O-].[O-]B([O-])[O-] LEZAYTDLNNEFJT-UHFFFAOYSA-N 0.000 claims description 4
- 235000013311 vegetables Nutrition 0.000 claims description 4
- PUKLDDOGISCFCP-JSQCKWNTSA-N 21-Deoxycortisone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2=O PUKLDDOGISCFCP-JSQCKWNTSA-N 0.000 claims description 3
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 3
- 235000008534 Capsicum annuum var annuum Nutrition 0.000 claims description 3
- 240000008384 Capsicum annuum var. annuum Species 0.000 claims description 3
- 240000008067 Cucumis sativus Species 0.000 claims description 3
- 235000016623 Fragaria vesca Nutrition 0.000 claims description 3
- 240000009088 Fragaria x ananassa Species 0.000 claims description 3
- 235000011363 Fragaria x ananassa Nutrition 0.000 claims description 3
- FCYKAQOGGFGCMD-UHFFFAOYSA-N Fulvic acid Natural products O1C2=CC(O)=C(O)C(C(O)=O)=C2C(=O)C2=C1CC(C)(O)OC2 FCYKAQOGGFGCMD-UHFFFAOYSA-N 0.000 claims description 3
- 235000011430 Malus pumila Nutrition 0.000 claims description 3
- 244000070406 Malus silvestris Species 0.000 claims description 3
- 235000015103 Malus silvestris Nutrition 0.000 claims description 3
- 244000061176 Nicotiana tabacum Species 0.000 claims description 3
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims description 3
- 240000007594 Oryza sativa Species 0.000 claims description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims description 3
- 235000009754 Vitis X bourquina Nutrition 0.000 claims description 3
- 235000012333 Vitis X labruscana Nutrition 0.000 claims description 3
- 240000006365 Vitis vinifera Species 0.000 claims description 3
- 235000014787 Vitis vinifera Nutrition 0.000 claims description 3
- 230000003213 activating effect Effects 0.000 claims description 3
- 235000019270 ammonium chloride Nutrition 0.000 claims description 3
- NGLMYMJASOJOJY-UHFFFAOYSA-O azanium;calcium;nitrate Chemical compound [NH4+].[Ca].[O-][N+]([O-])=O NGLMYMJASOJOJY-UHFFFAOYSA-O 0.000 claims description 3
- 229910021538 borax Inorganic materials 0.000 claims description 3
- 229910052796 boron Inorganic materials 0.000 claims description 3
- 229910000365 copper sulfate Inorganic materials 0.000 claims description 3
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 3
- 239000002509 fulvic acid Substances 0.000 claims description 3
- 229940095100 fulvic acid Drugs 0.000 claims description 3
- 229940099596 manganese sulfate Drugs 0.000 claims description 3
- 239000011702 manganese sulphate Substances 0.000 claims description 3
- 235000007079 manganese sulphate Nutrition 0.000 claims description 3
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 3
- -1 nitro humic acid Chemical compound 0.000 claims description 3
- WFIZEGIEIOHZCP-UHFFFAOYSA-M potassium formate Chemical compound [K+].[O-]C=O WFIZEGIEIOHZCP-UHFFFAOYSA-M 0.000 claims description 3
- GZWNUORNEQHOAW-UHFFFAOYSA-M potassium;2-aminoacetate Chemical compound [K+].NCC([O-])=O GZWNUORNEQHOAW-UHFFFAOYSA-M 0.000 claims description 3
- 235000009566 rice Nutrition 0.000 claims description 3
- 235000010339 sodium tetraborate Nutrition 0.000 claims description 3
- 239000004328 sodium tetraborate Substances 0.000 claims description 3
- 239000011573 trace mineral Substances 0.000 claims description 3
- 235000013619 trace mineral Nutrition 0.000 claims description 3
- 239000011701 zinc Substances 0.000 claims description 3
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 3
- 229960001763 zinc sulfate Drugs 0.000 claims description 3
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 3
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 235000012055 fruits and vegetables Nutrition 0.000 claims description 2
- 235000007686 potassium Nutrition 0.000 claims description 2
- 239000002994 raw material Substances 0.000 claims description 2
- 239000000463 material Substances 0.000 claims 3
- 244000241235 Citrullus lanatus Species 0.000 claims 1
- 244000098338 Triticum aestivum Species 0.000 claims 1
- 239000000969 carrier Substances 0.000 claims 1
- 230000007123 defense Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 19
- 201000010099 disease Diseases 0.000 abstract description 10
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 10
- 241000700605 Viruses Species 0.000 abstract description 4
- 238000005507 spraying Methods 0.000 abstract description 3
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 239000002671 adjuvant Substances 0.000 abstract 1
- 241000209140 Triticum Species 0.000 description 20
- 241000219109 Citrullus Species 0.000 description 13
- 238000012360 testing method Methods 0.000 description 12
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 description 10
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 10
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 description 10
- 239000012752 auxiliary agent Substances 0.000 description 8
- GVGUFUZHNYFZLC-UHFFFAOYSA-N dodecyl benzenesulfonate;sodium Chemical compound [Na].CCCCCCCCCCCCOS(=O)(=O)C1=CC=CC=C1 GVGUFUZHNYFZLC-UHFFFAOYSA-N 0.000 description 7
- 229940080264 sodium dodecylbenzenesulfonate Drugs 0.000 description 7
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical group OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 6
- SNRUBQQJIBEYMU-UHFFFAOYSA-N dodecane Chemical compound CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 239000002689 soil Substances 0.000 description 6
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 5
- 241000223218 Fusarium Species 0.000 description 5
- 238000009630 liquid culture Methods 0.000 description 5
- 235000021049 nutrient content Nutrition 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 230000004913 activation Effects 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 4
- 230000001965 increasing effect Effects 0.000 description 4
- 241000702308 Tomato yellow leaf curl virus Species 0.000 description 3
- 238000011068 loading method Methods 0.000 description 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 3
- 238000012807 shake-flask culturing Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241001024327 Oenanthe <Aves> Species 0.000 description 2
- 241000082085 Verticillium <Phyllachorales> Species 0.000 description 2
- 235000013877 carbamide Nutrition 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 239000002552 dosage form Substances 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 241000223600 Alternaria Species 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 241000228197 Aspergillus flavus Species 0.000 description 1
- 241001465180 Botrytis Species 0.000 description 1
- 235000009849 Cucumis sativus Nutrition 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000244355 Ligusticum Species 0.000 description 1
- 241001344133 Magnaporthe Species 0.000 description 1
- 241001344131 Magnaporthe grisea Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241001361634 Rhizoctonia Species 0.000 description 1
- 241000813090 Rhizoctonia solani Species 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000015784 hyperosmotic salinity response Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
Landscapes
- Fertilizers (AREA)
Abstract
The invention provides a water-soluble fertilizer, a preparation method and application thereof, and belongs to the technical field of agriculture. The preparation method comprises the following steps: mixing plant immunity protein composition, humic acid, nutrient and adjuvant, and stirring. The water-soluble fertilizer can be prepared into powder, water and granules, and can be applied in the modes of drip irrigation, spray irrigation, seed soaking, leaf surface spraying, root irrigation and flight protection during application. Meanwhile, the water-soluble fertilizer has the effects of effectively improving crop yield and promoting crop growth, and can also be used for preventing and treating plant virus diseases and improving sugar content in fruits.
Description
Technical Field
The invention belongs to the technical field of agriculture, and particularly relates to a water-soluble fertilizer and a preparation method and application thereof.
Background
The plant immunity protein is called as a plant immunity resistance inducer or a plant vaccine, has the effects of obviously preventing and treating diseases, resisting stress, increasing yield and improving quality, and is harmless to human and livestock and does not pollute the environment. The plant immunity protein does not directly act on a target, but excites the plant to generate an immune response, and induces the expression of plant related genes through a signal transduction system, so that corresponding defensive substances are generated, the immunity of the plant is improved, the rooting and the leaf enlargement of the plant are promoted, the drought resistance and the salt tolerance of the plant are enhanced, and meanwhile, the occurrence of plant diseases is lightened. The research and development of the plant immunity protein are the important development direction of the current international novel fertilizer and biological pesticide, and have great development prospect.
Fungal sources of plant immunity proteins are mainly Botrytis, alternaria, magnaporthe grisea (Magnaporthe), verticillium (Verticillium), aspergillus flavus (Aspergillus), rhizoctonia solani (Rhizoctonia), trichoderma, fusarium (Fusarium). Plant immunity proteins induce plants to develop systemic resistance, thereby activating the autoreactive system, and induce plant resistance for about 22 days.
How to better utilize the performance of plant immunity proteins, and provide fertilizer with good effect and wide functions, and further research is still needed.
Disclosure of Invention
In view of the above, the invention aims to provide a plant immunity protein water-soluble fertilizer, a preparation method and application thereof, and the water-soluble fertilizer can promote the growth of crops, improve the yield of the crops, can be used for preventing and treating plant virus diseases, and has the function of improving the sugar content in fruits.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the invention provides a plant immunity protein water-soluble fertilizer, which comprises a plant immunity protein composition, humic acid and nutrient substances.
Preferably, the plant immunity protein composition: humic acid: the weight percentage of the nutrient substances is 1-100: 1 to 100: 1-100, preferably a plant immunity protein composition: humic acid: the weight percentage of the nutrient substances is 1-77: 3 to 79:20 to 96 percent.
Preferably, the plant immunity protein composition comprises one or more of three active proteins PebC1, pebC2 and BcGS1 with molecular weights of 36kDa, 14kDa and 80kDa respectively.
Preferably, the humic acid comprises one or more of potassium fulvate, sodium humate, potassium humate, nitro humic acid, brown fulvic acid and boron humate.
Preferably, the nutrient substances comprise one or more of nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, medium element and trace element.
Preferably, the nutrient is selected from one or more of urea, urea ammonium nitrate solution, ammonium sulfate, ammonium chloride, ammonium polyphosphate, monoammonium phosphate, monopotassium phosphate, potassium chloride, potassium nitrate, potassium sulfate, potassium glycinate, potassium formate, calcium ammonium nitrate, magnesium chloride, zinc sulfate, zinc nitrate, zinc sulfate monohydrate, EDTA-Zn, sodium octaborate tetrahydrate, borax, magnesium sulfate, calcium nitrate, EDTA-Ca, copper sulfate, EDTA-Cu, manganese sulfate, EDTA-Mn, EDTA-Fe and ammonium molybdate.
Preferably, the preparation method of the plant immunity protein composition comprises the following steps:
activating Botrytis cinerea strain, culturing with seed solution, fermenting to obtain fermentation liquid, crushing fermentation liquid with colloid mill cell, heating, filtering, and purifying to obtain plant immunity protein composition.
Preferably, the preparation method of the water-soluble fertilizer comprises the following steps:
mixing plant immunity protein composition, humic acid and nutrient substances to prepare various dosage forms; preferably, 3-10% of humic acid, 20-50% of nutrient substances, 1% of disodium ethylenediamine tetraacetate and required auxiliary agents are added into the plant immunity protein composition, and the mixture is dried by a spray dryer, and then 1% of sodium dodecyl benzene sulfonate is added to prepare the solid water-soluble fertilizer;
preferably, 3-10% of humic acid, 20-50% of nutrient substances, 1% of disodium ethylenediamine tetraacetate and required auxiliary agents are added into the plant immunity protein composition, and then 1% of sodium dodecyl benzene sulfonate is added, so that the liquid water-soluble fertilizer can be prepared.
Preferably, the dosage form of the water-soluble fertilizer comprises powder, water and granules.
The invention provides application of the water-soluble fertilizer in improving crop growth performance and/or crop yield.
Preferably, the application method of the plant immunity protein water-soluble fertilizer comprises drip irrigation, spray irrigation, seed soaking, leaf surface spraying, root irrigation and flight protection.
Preferably, the field crop is selected from wheat, rice, corn, tobacco; the vegetables are selected from tomato, cucumber and green pepper, and the fruits are selected from strawberry, grape, apple and watermelon.
Compared with the prior art, the invention has the following beneficial effects:
(1) The water-soluble fertilizer prepared by the invention can provide nutrient substances required by the growth of crops, promote the rooting of the crops, ensure that the leaves of the crops are dark green after the crops are used, and the crops are strong, disease-resistant and stress-resistant, and improve the quality.
(2) The water-soluble fertilizer prepared by the invention has the function of preventing and treating plant virus diseases, such as tomato yellow leaf curl virus diseases, watermelon fusarium wilt and the like.
(3) The water-soluble fertilizer prepared by the invention can improve the yield of crops, vegetables and fruits (such as corn, wheat, tomatoes and the like) and can improve the sugar content in the fruits (such as watermelons).
Detailed Description
The invention provides a water-soluble fertilizer which comprises the following raw material components in parts by weight: 1-100 parts of plant immunity protein composition, 1-100 parts of humic acid and 1-100 parts of nutrient substances.
In the present invention, the amount of the plant immunity protein composition is 1 to 100 parts, preferably 1 to 77 parts, more preferably 30 to 60 parts, still more preferably 40 to 50 parts; the plant immunity protein composition comprises one or more of three active proteins PebC1, pebC2 and BcGS1 with molecular weights of 36kDa, 14kDa and 80kDa respectively. In the present invention, the method for preparing the plant immunity protein composition preferably comprises the following steps: fermenting the botrytis cinerea to obtain fermentation liquor, and carrying out colloid mill, filtration and purification treatment on the fermentation liquor to obtain the plant immunity protein composition. In the invention, the botrytis cinerea is subjected to activation, primary seed liquid culture and secondary seed liquid culture before liquid fermentation; the activation temperature is preferably 22 to 27 ℃, more preferably 24 to 26 ℃, still more preferably 25 ℃; the activation time is preferably 5 to 9d, more preferably 6 to 8d, still more preferably 7d; the temperature of the enrichment culture is preferably 22-27 ℃, more preferably 24-26 ℃, still more preferably 25 ℃; the time of the enrichment culture is preferably 70 to 75 hours, more preferably 72 to 74 hours, still more preferably 73 hours; the culture mode of the first-stage seed liquid is preferably shake flask culture, and the rotation speed of the shake flask culture is preferably 150-250 r/min, more preferably 170-230 r/min, and even more preferably 200r/min; the temperature of the primary seed liquid culture is preferably 22 to 27 ℃, more preferably 24 to 26 ℃, still more preferably 25 ℃; the culture time of the primary seed liquid is preferably 55-65 h, more preferably 58-63 h, still more preferably 60h; the culture temperature of the secondary seed liquid is the same as that of the primary seed liquid; the time for culturing the secondary seed liquid is preferably 70-75 hours, more preferably 72-74 hours, even more preferably 73 hours; the primary seed liquid culture and the secondary seed liquid culture are accompanied by stirring, and the stirring speed is preferably 90-110 r/min, more preferably 95-105 r/min, and even more preferably 100r/min; the fermentation broth is preferably mixed with potassium dihydrogen phosphate and dipotassium hydrogen phosphate before passing through a colloid mill; the final concentration of the phosphate buffer in the fermentation liquid after adding potassium dihydrogen phosphate and dipotassium hydrogen phosphate is preferably 15 to 25mM, more preferably 17 to 24mM, still more preferably 20mM; the fermentation broth is preferably subjected to heating treatment before filtration, and the heating temperature is preferably 90-110 ℃, more preferably 95-105 ℃, and even more preferably 100 ℃; the heating time is preferably 25 to 35 minutes, more preferably 27 to 32 minutes, and still more preferably 30 minutes. In the present invention, the effect of the heating is to destroy and inactivate other proteins. The purification method of the present invention is not particularly limited, and may be carried out by a method of purifying a protein which is conventional in the art. In the implementation process of the invention, the filtrate collected after filtration can be directly used.
In the present invention, the amount of the humic acid is 1 to 100 parts, preferably 3 to 79 parts, more preferably 30 to 60 parts, still more preferably 40 to 50 parts; the humic acid preferably comprises one or more of potassium fulvate, sodium humate, potassium humate and nitro humic acid, brown fulvic acid and boron humate, more preferably comprises one or more of potassium fulvate, sodium humate and potassium humate, and still more preferably comprises sodium humate; the nutrient preferably comprises one or more of nitrogen fertilizer, phosphate fertilizer, potash fertilizer, medium element and trace element, more preferably comprises one or more of nitrogen fertilizer, phosphate fertilizer and potash fertilizer, and still more preferably comprises nitrogen fertilizer.
In the present invention, the amount of the nutrient is 1 to 100 parts, preferably 20 to 96 parts, more preferably 30 to 60 parts, still more preferably 40 to 50 parts; the nutrient preferably comprises one or more of urea, urea ammonium nitrate solution, ammonium sulfate, ammonium chloride, ammonium polyphosphate, monoammonium phosphate, monopotassium phosphate, potassium chloride, potassium nitrate, potassium sulfate, potassium fulvate, potassium glycinate, potassium formate, calcium ammonium nitrate, magnesium chloride, zinc sulfate, zinc nitrate, zinc sulfate monohydrate, EDTA-Zn, sodium octaborate tetrahydrate, borax, magnesium sulfate, calcium nitrate, EDTA-Ca, copper sulfate, EDTA-Cu, manganese sulfate, EDTA-Mn, EDTA-Fe, ammonium molybdate, further preferably comprises one or more of urea, potassium dihydrogen phosphate, potassium fulvate, magnesium sulfate, EDTA-Ca, ammonium molybdate, still further preferably comprises one or more of ammonium sulfate, potassium chloride, magnesium chloride, zinc sulfate monohydrate.
The invention also provides a preparation method of the water-soluble fertilizer, which comprises the following steps:
mixing and stirring the plant immunity protein combination, humic acid and nutrient substances uniformly to obtain the water-soluble fertilizer.
The specific type of the auxiliary agent is not particularly limited, and the preparation formulation of the water-soluble fertilizer is specifically selected according to the preparation. When preparing the solid water-soluble fertilizer, the preparation method is as follows:
drying the plant immunity protein composition, humic acid, nutrient substances, 1% of disodium ethylenediamine tetraacetate and required auxiliary agents by a spray dryer, and adding 1% of sodium dodecyl benzene sulfonate to prepare a solid water-soluble fertilizer;
when preparing the liquid water-soluble fertilizer, the preparation method is as follows:
humic acid, nutrient substances, 1% of disodium ethylenediamine tetraacetate and required auxiliary agents are added into the plant immunity protein composition, and then 1% of sodium dodecyl benzene sulfonate is added, so that the liquid water-soluble fertilizer can be prepared. In the preparation of the liquid water-soluble fertilizer, the auxiliary agent is preferably ethylene glycol.
In the present invention, the amount of sodium dodecylbenzenesulfonate added is preferably 0.5 to 1.5 parts, more preferably 0.8 to 1.2 parts, and still more preferably 1.0 part.
The invention also provides application of the water-soluble fertilizer in promoting crop growth.
In the present invention, the crops preferably include field crops, fruits and vegetables; the field crops preferably comprise wheat, rice, corn and tobacco; the fruit preferably comprises strawberry, grape, apple, watermelon; the vegetables preferably comprise tomatoes, cucumbers and green peppers.
The invention also provides application of the water-soluble fertilizer in preventing and treating plant virus diseases.
The invention also provides application of the water-soluble fertilizer in improving crop yield and sugar content.
In the invention, the water-soluble fertilizer is applied in the modes of drip irrigation, spray irrigation, seed soaking, leaf surface spraying, root irrigation and flight protection during use. The concentration of the water-soluble fertilizer is preferably 800-1200 times of dilution, more preferably 900-1100 times of dilution, and even more preferably 1000 times of dilution.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
EXAMPLE 1 preparation of plant immune proteins
(1) Inoculating a botrytis cinerea strain on a PDA culture medium, and performing activation culture for 7d at 25 ℃; meanwhile, the culture medium is sub-packaged in 1000ml triangular flasks for sterilization, and the liquid loading amount of each triangular flask is 500ml;
(2) Inoculating the activated strain into a triangular flask, and shake-flask culturing at 26 ℃ and 200r/min for 72 hours to obtain primary seed liquid;
(3) Inoculating 1L of primary seed liquid cultured in a triangular flask into a 500L fermentation tank, wherein the liquid loading amount in the fermentation tank is 300L, and culturing at 26 ℃ for 60h at 100r/min to obtain secondary seed liquid;
(4) According to the following steps of 1:10, inoculating the secondary seed liquid into a fermentation tank with the liquid loading amount of 2/3, and culturing at 26 ℃ for 72 hours at 100r/min to obtain fermentation liquid;
(5) Adding monopotassium phosphate and dipotassium phosphate into the fermentation liquor to obtain a phosphate buffer solution with the final concentration of 20mM, crushing cells by using three groups of colloid mills, heating at 100 ℃ for 30min, and filtering and purifying to obtain the plant immunity protein composition.
Example 2 preparation of Water-soluble Fertilizer powder
The plant immunity protein composition prepared in the embodiment 1 is added with potassium humate, urea, monoammonium phosphate, potassium nitrate, disodium ethylenediamine tetraacetate and required auxiliary agents according to mass percent, and the mixture is uniformly mixed and stirred, filtered, dried by a spray dryer, and added with sodium dodecyl benzene sulfonate, thus obtaining the plant immunity protein macroelement water-soluble fertilizer powder.
Wherein the plant immunity protein composition comprises 57.8% of plant immunity protein composition, 5% of potassium humate, 16.8% of urea, 8.4% of monoammonium phosphate, 10% of potassium nitrate, 1% of disodium ethylenediamine tetraacetate and 1% of dodecane basic sodium sulfonate.
Example 3 preparation of Water-soluble Fertilizer powder
The preparation method comprises the following steps: same as in example 2
Wherein the plant immunity protein composition comprises 55.7% of plant immunity protein composition, 8.5% of humic acid potassium, 21.8% of urea, 10% of monopotassium phosphate, 2% of potassium sulfate, 1% of disodium ethylenediamine tetraacetate and 1% of dodecane basic sodium sulfonate.
Example 4 preparation of Water-soluble fertilizer aqua
Adding potassium humate, urea, monoammonium phosphate, potassium nitrate, disodium ethylenediamine tetraacetate and required auxiliary agents into the plant immunity protein composition according to mass percent, finally adding sodium dodecyl benzene sulfonate, mixing and stirring uniformly, and filtering to prepare the plant immunity protein macroelement water-soluble fertilizer aqueous agent.
Wherein the plant immunity protein composition comprises 53.7% of plant immunity protein composition, 4.5% of potassium humate, 26% of urea ammonium nitrate, 4.2% of ammonium polyphosphate, 9.6% of dipotassium hydrogen phosphate, 1% of disodium ethylene diamine tetraacetate and 1% of ethylene glycol.
Example 5 preparation of Water-soluble Fertilizer powder
The preparation method comprises the following steps: same as in example 2
Wherein the plant immunity protein composition comprises 59.6% of plant immunity protein composition, 6.8% of potassium humate, 8.7% of zinc sulfate monohydrate, 4% of EDTA-Mn, 5.6% of EDTA-Cu, 8% of EDTA-Fe, 0.4% of ammonium molybdate, 4.9% of sodium octaborate tetrahydrate, 1% of disodium ethylenediamine tetraacetate and 1% of dodecane basic sodium sulfonate.
Test example 1 Effect of Water-soluble fertilizer on wheat growth and yield
2 mu of test wheat fields (pH 6.97, nitrogen content of 150mg/kg, phosphorus content of 150g/kg, potassium content of 287g/kg and organic matter of 30.74 g/kg) with the same soil type and nutrient content are selected from test wheat fields of the agricultural institute of ligusticum city in Hebei province, and wheat is planted. Respectively applying water-soluble fertilizer in the wheat green-returning and jointing period and the booting and heading period; the water-soluble fertilizer obtained in example 2 and example 3 was applied as a control group (CK) which was purchased conventionally in the market. The water-soluble fertilizer is applied in an amount of 15 g/(mu. Times), diluted 1000 times, applied at an interval of 20 days, sprayed 3 times, and applied in a total amount of 45 g per mu. The results are shown in tables 1-1, 1-2 and 2.
TABLE 1-1 Effect of Water-soluble fertilizers on wheat seedlings
TABLE 1-2 Effect of Water-soluble fertilizers on wheat seedlings
TABLE 2 Effect of Water-soluble fertilizers on wheat yield
As can be seen from tables 1-1, 1-2 and 2, after the water-soluble fertilizer prepared by the invention is applied, compared with a blank control, wheat seedlings grow in order, plants are strong, root systems are developed, tillering numbers are large, the stress resistance, cold resistance and drought resistance are strong, and the green turning is fast; the wheat in the flowering period is large in wheat ears, full in seeds, high in lodging resistance and thousand seed weight, and obvious in yield increasing effect.
Test example 2 Effect of Water-soluble fertilizer on wheat yield
In the test wheat fields in the Shahew towns of the Chen Tai, hebei, 3 mu of test wheat fields with basically the same soil type and nutrient content are selected, and wheat is planted. Applying a water-soluble fertilizer in the booting and heading period of wheat; the water-soluble fertilizer prepared in application example 3 was used as treatment group 1, the water-soluble fertilizer prepared in application example 2 was used as treatment 2, and the water-soluble fertilizer was not used as control group (CK). The application amount of the water-soluble fertilizer is 25 g/mu each time, the water-soluble fertilizer is diluted 1000 times for use, the use time interval is 7 days, the unmanned aerial vehicle sprays for 2 times, and the total fertilization amount per mu is 50 g. The results are shown in Table 3.
TABLE 3 Effect of Water-soluble fertilizers on wheat yield
As can be seen from Table 3, after the water-soluble fertilizer prepared by the invention is applied, wheat ears are dark green and glossy, the growth vigor is strong, the wheat stalks are strong, and the yield-increasing effect is expected to be obvious.
Test example 3 Effect of Water-soluble fertilizers on maize plants and yield
In the test corn fields in Wucheng county of Shandong province, 2 mu of test corn fields with basically same soil type and nutrient content are selected, and corn of Zhongdan 909 is planted. The conventional water-soluble fertilizer purchased on the market was used as a control CK, and the water-soluble fertilizer prepared in example 2 was used as a test treatment. The corn seedlings were applied 1 time in foliar spray and 1 time in foliar spray after 25 days. Wherein the application amount of the water-soluble fertilizer is 25 g/mu, the dilution is 1000 times, and the total fertilization amount per mu is 50 g. The results are shown in tables 4 and 5.
TABLE 4 Effect of Water-soluble fertilizers on maize plants
TABLE 5 Effect of Water-soluble fertilizers on corn yield
As can be seen from tables 4 and 5, after the water-soluble fertilizer prepared by the invention is applied, the leaves of the corn at the seedling stage are dark green, the plants are strong, and the leaves are wide; the corn ear length in the harvest period increases the number of grains in rows and the thousand grain weight, the length of the bald tip is reduced, and the yield is obviously increased.
Test example 4 Effect of Water-soluble fertilizer on tomato yellow leaf curl Virus disease control and yield
In the greenhouse of modern agriculture and technology garden in Hebei province, selecting soil with basically same soil type and nutrient content, and planting tomatoes with the variety of golden greenhouse autumn. Applying the water-soluble fertilizer prepared in the example 2 and a commercially available water-soluble fertilizer in a tomato seedling stage; and the water-soluble fertilizer prepared in application example 2 is used as a treatment group 1, and the water-soluble fertilizer of a company conventionally purchased in the market is used as a treatment group control CK. The application amount of the water-soluble fertilizer prepared by the method is 15 g/mu each time, the water-soluble fertilizer is diluted 1000 times and used, the foliar spray is carried out for 3 times, and the application interval of each time is about 20 days. The results are shown in tables 6 and 7.
TABLE 6 prevention and treatment effects of Water-soluble fertilizer on tomato yellow leaf curl virus disease
TABLE 7 Effect of Water soluble fertilizers on tomato yield
As can be seen from tables 6 and 7, after the water-soluble fertilizer prepared by the invention is applied, the leaves of tomato seedlings are dark green, the plants are strong, the fruit setting rate is high, and the disease resistance is enhanced; the tomato fruits in the mature period are round, bright in color, good in taste and obviously increased in yield.
Test example 5 Effect of Water-soluble fertilizer on control of watermelon fusarium wilt and sugar content
In the experimental watermelon greenhouse of Rao Yang county in Hebei province, selecting an experimental field with basically same soil type and nutrient content, and planting watermelons. Applying the water-soluble fertilizer prepared in example 2 and a commercially available water-soluble fertilizer in the mature period of the watermelon; the water-soluble fertilizer prepared in application example 2 was used as treatment group 1, and the conventional water-soluble fertilizer purchased in the market was used as control group (CK), and was used as treatment group 2. The application amount of the water-soluble fertilizer is 20 g/mu each time, the water-soluble fertilizer is diluted 1000 times and used, the foliar spray is carried out for 2 times, and the application interval of each time is 25 days. The results are shown in Table 8.
Table 8 effects of Water-soluble fertilizers on controlling sugar content of watermelon and fusarium wilt of watermelon
As can be seen from Table 8, after the water-soluble fertilizer prepared by the invention is applied, the watermelon leaves are dark green, the plant is strong, and the fruit setting rate is high; the application in the mature period can advance the time of watermelon marketing, the pulp of the watermelon is ruddy, the sugar content is high, and the taste is good.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (10)
1. The water-soluble fertilizer is characterized by comprising the following raw material components in parts by weight: 1-100 parts of plant immunity protein composition, 1-100 parts of humic acid and 1-100 parts of nutrient substances;
the plant immunity protein composition comprises one or more of PebC1, pebC2 and BcGS 1.
2. The water-soluble fertilizer of claim 1, wherein the humic acid comprises one or more of potassium fulvate, sodium humate, potassium humate, nitro humic acid, brown fulvic acid, boron humate.
3. The water-soluble fertilizer of claim 1, wherein the nutrient comprises one or more of a nitrogen fertilizer, a phosphate fertilizer, a potash fertilizer, a medium element, and a trace element.
4. The water-soluble fertilizer of claim 1, wherein the nutrients comprise one or more of urea, urea ammonium nitrate solution, ammonium sulfate, ammonium chloride, ammonium polyphosphate, monoammonium phosphate, monopotassium phosphate, potassium chloride, potassium nitrate, potassium sulfate, potassium fulvate, potassium glycinate, potassium formate, calcium ammonium nitrate, magnesium chloride, zinc sulfate, zinc nitrate, zinc sulfate monohydrate, EDTA-Zn, sodium octaborate tetrahydrate, borax, magnesium sulfate, calcium nitrate, EDTA-Ca, copper sulfate, EDTA-Cu, manganese sulfate, EDTA-Mn, EDTA-Fe, ammonium molybdate.
5. The water-soluble fertilizer according to claim 1, wherein the method for preparing the plant immunity protein composition comprises the following steps:
activating Botrytis cinerea strain, culturing with seed solution, fermenting to obtain fermentation liquid, crushing fermentation liquid with colloid mill cell, heating, filtering, and purifying to obtain plant immunity protein composition.
6. The method for preparing a water-soluble fertilizer according to any one of claims 1 to 5, comprising the steps of:
humic acid and nutrient substances are added into the plant immunity protein composition, and the water-soluble fertilizer is obtained after uniform mixing and stirring.
7. The method of claim 6, wherein the water-soluble fertilizer comprises a powder, a water or a granule;
auxiliary materials are added in the preparation process of the powder, the water agent or the granule, and the auxiliary materials comprise auxiliary materials or carriers.
8. Use of a water-soluble fertilizer as claimed in any one of claims 1 to 5 for improving crop growth performance and/or crop yield.
9. The use according to claim 8, wherein the water-soluble fertilizer is applied in the form of drip irrigation, spray irrigation, seed soaking, foliar spray, root irrigation and air defense at the time of use.
10. The use according to claim 8, wherein the crops include field crops, fruits and vegetables; the field crops comprise wheat, rice, corn and tobacco; the fruit comprises strawberry, grape, apple and watermelon; the vegetables include tomato, cucumber and green pepper.
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| CN111138231A (en) * | 2019-11-19 | 2020-05-12 | 西安交大凯达新技术有限责任公司 | Humic acid-containing high-concentration liquid water-soluble fertilizer and preparation method thereof |
| CN113603535A (en) * | 2021-08-19 | 2021-11-05 | 中农科生物工程技术(苏州)有限公司 | Combined water-soluble fertilizer and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104738094A (en) * | 2013-12-31 | 2015-07-01 | 北京绿色农华植保科技有限责任公司 | Plant immune induced resistance protein composition as well as preparation method and application thereof |
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