CN116584576A - Method for mixed fermentation of feed yeast protein particles by amino acid mother liquor and corn steep liquor - Google Patents
Method for mixed fermentation of feed yeast protein particles by amino acid mother liquor and corn steep liquor Download PDFInfo
- Publication number
- CN116584576A CN116584576A CN202310600661.7A CN202310600661A CN116584576A CN 116584576 A CN116584576 A CN 116584576A CN 202310600661 A CN202310600661 A CN 202310600661A CN 116584576 A CN116584576 A CN 116584576A
- Authority
- CN
- China
- Prior art keywords
- liquor
- fermentation
- amino acid
- culturing
- corn steep
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000855 fermentation Methods 0.000 title claims abstract description 98
- 230000004151 fermentation Effects 0.000 title claims abstract description 98
- 240000008042 Zea mays Species 0.000 title claims abstract description 65
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 title claims abstract description 65
- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 65
- 235000005822 corn Nutrition 0.000 title claims abstract description 65
- 150000001413 amino acids Chemical class 0.000 title claims abstract description 49
- 239000012452 mother liquor Substances 0.000 title claims abstract description 43
- 239000002245 particle Substances 0.000 title claims abstract description 36
- 108010058643 Fungal Proteins Proteins 0.000 title claims abstract description 35
- 238000000034 method Methods 0.000 title claims abstract description 33
- 238000012258 culturing Methods 0.000 claims abstract description 82
- 230000001954 sterilising effect Effects 0.000 claims abstract description 49
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 43
- 239000007921 spray Substances 0.000 claims abstract description 35
- 238000001816 cooling Methods 0.000 claims abstract description 31
- 238000001035 drying Methods 0.000 claims abstract description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- 238000002156 mixing Methods 0.000 claims abstract description 21
- 239000000758 substrate Substances 0.000 claims abstract description 11
- 229920001817 Agar Polymers 0.000 claims abstract description 10
- 239000008272 agar Substances 0.000 claims abstract description 10
- 239000001963 growth medium Substances 0.000 claims description 54
- 239000007788 liquid Substances 0.000 claims description 50
- 239000000243 solution Substances 0.000 claims description 29
- 238000003756 stirring Methods 0.000 claims description 21
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 19
- 238000001704 evaporation Methods 0.000 claims description 19
- 239000008103 glucose Substances 0.000 claims description 19
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 18
- 230000008030 elimination Effects 0.000 claims description 18
- 238000003379 elimination reaction Methods 0.000 claims description 18
- 239000002054 inoculum Substances 0.000 claims description 18
- 238000009630 liquid culture Methods 0.000 claims description 18
- 235000019750 Crude protein Nutrition 0.000 claims description 17
- 239000000843 powder Substances 0.000 claims description 13
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 claims description 12
- 239000002918 waste heat Substances 0.000 claims description 12
- 235000019764 Soybean Meal Nutrition 0.000 claims description 10
- 239000004455 soybean meal Substances 0.000 claims description 10
- 239000001888 Peptone Substances 0.000 claims description 9
- 108010080698 Peptones Proteins 0.000 claims description 9
- 108010009736 Protein Hydrolysates Proteins 0.000 claims description 9
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 9
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 9
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 9
- 229940041514 candida albicans extract Drugs 0.000 claims description 9
- 239000013530 defoamer Substances 0.000 claims description 9
- 239000012153 distilled water Substances 0.000 claims description 9
- 230000008020 evaporation Effects 0.000 claims description 9
- 239000012530 fluid Substances 0.000 claims description 9
- 238000005469 granulation Methods 0.000 claims description 9
- 230000003179 granulation Effects 0.000 claims description 9
- 229940095686 granule product Drugs 0.000 claims description 9
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 9
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 9
- 239000010413 mother solution Substances 0.000 claims description 9
- 235000019319 peptone Nutrition 0.000 claims description 9
- 238000005086 pumping Methods 0.000 claims description 9
- 238000011218 seed culture Methods 0.000 claims description 9
- 239000007787 solid Substances 0.000 claims description 9
- 239000012138 yeast extract Substances 0.000 claims description 9
- 239000004472 Lysine Substances 0.000 claims description 8
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 8
- 239000004473 Threonine Substances 0.000 claims description 5
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims description 5
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims description 3
- 241000235649 Kluyveromyces Species 0.000 claims description 3
- 150000002148 esters Chemical class 0.000 claims description 3
- 239000003205 fragrance Substances 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 9
- 239000006227 byproduct Substances 0.000 abstract description 5
- 239000008187 granular material Substances 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 4
- 239000000463 material Substances 0.000 abstract description 3
- 239000002994 raw material Substances 0.000 abstract description 3
- 150000003839 salts Chemical class 0.000 abstract description 3
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- 238000004806 packaging method and process Methods 0.000 abstract description 2
- 230000008569 process Effects 0.000 description 7
- 239000000047 product Substances 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 238000005265 energy consumption Methods 0.000 description 3
- 238000002791 soaking Methods 0.000 description 3
- 238000001694 spray drying Methods 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 241001052560 Thallis Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- YNPNZTXNASCQKK-UHFFFAOYSA-N phenanthrene Chemical compound C1=CC=C2C3=CC=CC=C3C=CC2=C1 YNPNZTXNASCQKK-UHFFFAOYSA-N 0.000 description 2
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 239000005996 Blood meal Substances 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000004467 fishmeal Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
- Botany (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a method for fermenting feed yeast protein particles by mixing amino acid mother liquor and corn steep liquor, which comprises the following steps: step a: culturing yeast plates: preparing a YEPD agar medium, sterilizing at 121deg.C for 30min, and cooling to obtain a plate medium. The invention only uses the byproduct amino acid mother liquor and corn steep liquor of corn deep processing enterprises as fermentation substrates for industrialized production, does not add other fermentation nutrition auxiliary materials and inorganic salts, reduces the cost of raw materials, only uses the byproduct amino acid mother liquor and corn steep liquor of the corn deep processing enterprises as fermentation substrates for industrialized production, does not need to adjust the pH value of the fermentation substrates by alkaline chemicals, has 58-65% of dry matter content after concentration of the fermentation liquor, has very low water content, reduces the steam consumption in the drying process, saves the production cost, adopts a spray fluidized bed for drying and granulating, and has high volume weight and 0.7-1.7mm diameter of granules, thereby being more convenient for packaging and transportation and more convenient for feeding by feed enterprises.
Description
Technical Field
The invention relates to the technical field of microorganism submerged fermentation, in particular to a method for fermenting feed yeast protein particles by mixing amino acid mother liquor and corn steep liquor.
Background
The amino acid mother liquor and corn steep liquor are byproducts of corn deep processing enterprises, the amino acid mother liquor is mother liquor generated in the process of producing lysine and threonine by fermentation, contains part of mycoprotein, mineral substances and lysine or threonine remained in the extraction process, the corn steep liquor is produced by concentrating soaking liquid generated in the soaking process before corn crushing by an evaporator, contains protein, mineral substances, organic phosphorus and various amino acids, the pH value of the amino acid mother liquor and the corn steep liquor is 3.8-4.5 and is slightly acidic, COD is about 10 ten thousand mg/l, NH3-N is about 1000mg/l, the amino acid mother liquor and the corn steep liquor belong to high-concentration organic sewage, the treatment cost is high, the existing enterprises spray the amino acid mother liquor and the corn steep liquor into carriers such as corn husks, corn germ meal and peanut shells to produce spray products, and the spray products are used as feed raw materials at low end, the energy consumption in the drying process is high, and the added value is low.
The yeast has rich protein, nucleic acid, amino acid, vitamin and other contents, aromatic smell, and has the functions of easy absorption, food calling and other probiotics, and can replace high-value protein such as fermented soybean meal, blood meal, fish meal and the like partially, thereby reducing the cost of the feed and having considerable economic benefit.
Chinese patent CN101709272B describes a preparation method of feed yeast, which uses waste liquid after extracting feitin with corn soaking water as yeast culture substrate, uses liquid ammonia to adjust pH, uses glucose mother liquor or liquid sugar or other molasses as carbon source, and obtains feed yeast powder product by evaporating concentration and centrifugal spray drying; in the process, the ion exchange resin is adopted to extract the phenanthrene, and acid and alkali are required to elute and regenerate the resin in the production process, so that a large amount of salt-containing wastewater is generated; the ammonium salt is formed in the process of adjusting the pH by adopting liquid ammonia, so that the quality of the feed yeast powder is reduced, and the feed yeast powder cannot be used in a large amount in pig feed and poultry feed; the fermentation liquor is subjected to spray drying by adopting a centrifugal spray drying tower, so that the drying energy consumption is high, and the finished product is powder with low volume weight, which is not beneficial to being added into feed.
Chinese patent CN104726338B describes a method for producing granule proteins by using lysine mother liquor, wherein the lysine mother liquor is heated to 100 ℃, then the lower layer thalli and the upper layer liquid are separated and collected by a centrifuge, the thalli are hydrolyzed by hydrochloric acid, the cell walls are separated by the centrifuge, and the supernatant is concentrated and dried to form granule proteins; adding potassium humate, diatomite and the like into the upper liquid to prepare a compound fertilizer; the process is only physical separation of lysine mother liquor, does not carry out advanced treatment, and has a complex process flow.
Chinese patent CN113455584B describes a method for producing fodder yeast particles using corn steep liquor, which only uses corn steep liquor as a fermentation substrate to cultivate yeast, and the corn steep liquor is not subjected to actual or continuous sterilization; the corn steep liquor has low total sugar content per se, and has the problem of low yeast quantity caused by insufficient carbon source; the corn steep liquor is not subjected to actual sterilization or continuous sterilization, when the pH value is raised to 4.5-5.0 in the fermentation process, the mixed bacteria of the corn steep liquor can grow gradually, the quality and the flavor of the final finished product are affected, and the method for fermenting the feed yeast protein particles by mixing the amino acid mother liquor and the corn steep liquor is purposefully provided due to the problems.
Disclosure of Invention
The invention aims to provide a method for fermenting feed yeast protein particles by mixing amino acid mother liquor and corn steep liquor, which has the advantages of simplifying process flow and auxiliary material consumption, reducing energy consumption for concentration and drying, and solving the problems in the background art.
In order to achieve the above purpose, the present invention provides the following technical solutions: the method for fermenting the feed yeast protein particles by mixing the amino acid mother liquor and the corn steep liquor comprises the following steps:
step a: culturing yeast plates: preparing a YEPD agar culture medium, sterilizing at 121deg.C for 30min, cooling to obtain a plate culture medium, inoculating yeast to the plate culture medium in a sterile room, and culturing in a biochemical incubator at 31deg.C for 16-18 hr;
step b: preparing shake flask seed liquid: 30g of peptone, 20g of yeast extract powder, 30g of glucose, 2000g of distilled water and natural pH, sterilizing at 121 ℃ for 30min by a sterilizing pot, preparing a shake flask liquid culture medium, inoculating the prepared yeast flat plate into a shake flask in a sterile room, and delivering the shake flask liquid culture medium to shake flask for culturing at 31 ℃ for 20-22h at a shaking table rotating speed of 200r/min and an OD (10 times 600 nm) of about 1.6 after culturing to obtain shake flask seed liquid;
step c: preparing primary seed liquid: taking 1Kg of glucose, 0.1Kg of soybean meal hydrolysate, 5Kg of corn steep liquor, 2g of magnesium sulfate, 10g of ammonium sulfate and 10g of defoamer, preparing a first-stage seed culture medium after sterilizing at 121 ℃ for 60min and cooling, transferring the prepared shake flask seed liquid to a seed tank, stirring, ventilating and culturing, wherein the inoculum size is 0.1-0.2%, the culturing temperature is 30-38 ℃ and the time is 20-22h, and the OD (10 times 600 nm) is about 1.6 after culturing is finished, thus obtaining the first-stage seed liquid;
step d: industrial culture in a fermentation tank: uniformly mixing an amino acid mother solution and corn steep liquor in a ratio of 1:1 to 1:10, sterilizing by a solid elimination or continuous elimination system, pumping into a fermentation tank, cooling to 34 ℃, naturally adjusting pH, transferring the prepared primary seed solution to the fermentation tank, stirring, ventilating and culturing, wherein the inoculum size is 0.2-0.5%, the culturing temperature is 30-38 ℃, the fermentation time is 22-26h, and the fermentation end point pH value is 6.5-6.8;
step e: and (3) evaporating and concentrating: d, vacuum and low-temperature evaporation concentration is carried out on the fermentation liquor with the pH value of 6.5-6.8 produced in the step d by adopting a waste heat or MVR evaporator, and the dry matter content of the concentrated liquor is 58-65%;
step f: spray fluidized bed drying granulation: and c, drying and granulating the concentrated solution obtained in the step e by a two-fluid spray gun (air+concentrated solution) in a spray fluidized bed to obtain a feed yeast protein granule product, wherein the crude protein content is more than or equal to 53%, the water content is less than or equal to 10%, and the particle size is 0.7-1.7mm.
Preferably, the yeast is selected from one or two of candida, kluyveromyces or isaccharomyces.
Preferably, the fermentation tank used in the step d is a mechanically stirring and ventilating fermentation tank, and the amino acid mother liquor used in the step d is one or two of threonine mother liquor and lysine mother liquor and is mixed with corn steep liquor to form a fermentation culture substrate of the yeast.
Preferably, the evaporator adopted in the step e is a waste heat or MVR multi-effect forced circulation evaporator, the fermentation liquor is highly concentrated, the dry matter content of the obtained concentrated liquor is 58-65%, and the temperature of the concentrated liquor is 65-75 ℃.
Preferably, the fermentation liquor crude protein at the fermentation end point in the step d is more than or equal to 53%, the total amino acid is more than or equal to 48%, and the fermentation liquor crude protein has ester fragrance.
Preferably, in the step f, drying and granulating are carried out by adopting a spray fluidized bed, and the yeast protein particles are subjected to vibration screening and then cooled by a cooling fluidized bed to obtain the feed yeast protein particles with the diameter of 0.7-1.7mm, wherein the crude protein is more than or equal to 53%, the total amino acid is more than or equal to 48%, and the water content is less than or equal to 10%.
Compared with the prior art, the invention has the following beneficial effects:
the invention only uses the byproduct amino acid mother liquor and corn steep liquor of corn deep processing enterprises as fermentation substrates for industrialized production, does not add other fermentation nutrition auxiliary materials and inorganic salts, reduces the cost of raw materials, only uses the byproduct amino acid mother liquor and corn steep liquor of the corn deep processing enterprises as fermentation substrates for industrialized production, does not need to adjust the pH value of the fermentation substrates by alkaline chemicals, has 58-65% of dry matter content after concentration of the fermentation liquor, has very low water content, reduces the steam consumption in the drying process, saves the production cost, adopts a spray fluidized bed for drying and granulating, and has high volume weight and 0.7-1.7mm diameter of granules, thereby being more convenient for packaging and transportation and more convenient for feeding by feed enterprises.
Detailed Description
The following description of the technical solutions in the embodiments of the present invention will be clear and complete, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The method for fermenting the feed yeast protein particles by mixing the amino acid mother liquor and the corn steep liquor comprises the following steps:
step a: culturing yeast plates: preparing a YEPD agar culture medium, sterilizing at 121deg.C for 30min, cooling to obtain a plate culture medium, inoculating yeast to the plate culture medium in a sterile room, and culturing in a biochemical incubator at 31deg.C for 16-18 hr;
step b: preparing shake flask seed liquid: 30g of peptone, 20g of yeast extract powder, 30g of glucose, 2000g of distilled water and natural pH, sterilizing at 121 ℃ for 30min by a sterilizing pot, preparing a shake flask liquid culture medium, inoculating the prepared yeast flat plate into a shake flask in a sterile room, and delivering the shake flask liquid culture medium to shake flask for culturing at 31 ℃ for 20-22h at a shaking table rotating speed of 200r/min and an OD (10 times 600 nm) of about 1.6 after culturing to obtain shake flask seed liquid;
step c: preparing primary seed liquid: taking 1Kg of glucose, 0.1Kg of soybean meal hydrolysate, 5Kg of corn steep liquor, 2g of magnesium sulfate, 10g of ammonium sulfate and 10g of defoamer, preparing a first-stage seed culture medium after sterilizing at 121 ℃ for 60min and cooling, transferring the prepared shake flask seed liquid to a seed tank, stirring, ventilating and culturing, wherein the inoculum size is 0.1-0.2%, the culturing temperature is 30-38 ℃ and the time is 20-22h, and the OD (10 times 600 nm) is about 1.6 after culturing is finished, thus obtaining the first-stage seed liquid;
step d: industrial culture in a fermentation tank: uniformly mixing an amino acid mother solution and corn steep liquor in a ratio of 1:1 to 1:10, sterilizing by a solid elimination or continuous elimination system, pumping into a fermentation tank, cooling to 34 ℃, naturally adjusting pH, transferring the prepared primary seed solution to the fermentation tank, stirring, ventilating and culturing, wherein the inoculum size is 0.2-0.5%, the culturing temperature is 30-38 ℃, the fermentation time is 22-26h, and the fermentation end point pH value is 6.5-6.8;
step e: and (3) evaporating and concentrating: d, vacuum and low-temperature evaporation concentration is carried out on the fermentation liquor with the pH value of 6.5-6.8 produced in the step d by adopting a waste heat or MVR evaporator, and the dry matter content of the concentrated liquor is 58-65%;
step f: spray fluidized bed drying granulation: and c, drying and granulating the concentrated solution obtained in the step e by a two-fluid spray gun (air+concentrated solution) in a spray fluidized bed to obtain a feed yeast protein granule product, wherein the crude protein content is more than or equal to 53%, the water content is less than or equal to 10%, and the particle size is 0.7-1.7mm.
Example 1
The method for fermenting the feed yeast protein particles by mixing the amino acid mother liquor and the corn steep liquor comprises the following steps:
step a: culturing yeast plates: preparing a YEPD agar culture medium, sterilizing at 121deg.C for 30min, cooling to obtain a plate culture medium, inoculating yeast to the plate culture medium in a sterile room, and culturing in a biochemical incubator at 31deg.C for 16-18 hr;
step b: preparing shake flask seed liquid: 30g of peptone, 20g of yeast extract powder, 30g of glucose, 2000g of distilled water and natural pH, sterilizing at 121 ℃ for 30min by a sterilizing pot, preparing a shake flask liquid culture medium, inoculating the prepared yeast flat plate into a shake flask in a sterile room, and delivering the shake flask liquid culture medium to shake flask for culturing at 31 ℃ for 20-22h at a shaking table rotating speed of 200r/min and an OD (10 times 600 nm) of about 1.6 after culturing to obtain shake flask seed liquid;
step c: preparing primary seed liquid: taking 1Kg of glucose, 0.1Kg of soybean meal hydrolysate, 5Kg of corn steep liquor, 2g of magnesium sulfate, 10g of ammonium sulfate and 10g of defoamer, preparing a first-stage seed culture medium after sterilizing at 121 ℃ for 60min and cooling, transferring the prepared shake flask seed liquid to a seed tank, stirring, ventilating and culturing, wherein the inoculum size is 0.1-0.2%, the culturing temperature is 30-38 ℃ and the time is 20-22h, and the OD (10 times 600 nm) is about 1.6 after culturing is finished, thus obtaining the first-stage seed liquid;
step d: industrial culture in a fermentation tank: uniformly mixing an amino acid mother solution and corn steep liquor in a ratio of 1:1 to 1:10, sterilizing by a solid elimination or continuous elimination system, pumping into a fermentation tank, cooling to 34 ℃, naturally adjusting pH, transferring the prepared primary seed solution to the fermentation tank, stirring, ventilating and culturing, wherein the inoculum size is 0.2-0.5%, the culturing temperature is 30-38 ℃, the fermentation time is 22-26h, and the fermentation end point pH value is 6.5-6.8;
step e: and (3) evaporating and concentrating: d, vacuum and low-temperature evaporation concentration is carried out on the fermentation liquor with the pH value of 6.5-6.8 produced in the step d by adopting a waste heat or MVR evaporator, and the dry matter content of the concentrated liquor is 58-65%;
step f: spray fluidized bed drying granulation: and c, drying and granulating the concentrated solution obtained in the step e by a two-fluid spray gun (air+concentrated solution) in a spray fluidized bed to obtain a feed yeast protein granule product, wherein the crude protein content is more than or equal to 53%, the water content is less than or equal to 10%, and the particle size is 0.7-1.7mm.
Example 2
In example 1, the following steps are added:
the yeast is one or two selected from candida, kluyveromyces and isatus.
The method for fermenting the feed yeast protein particles by mixing the amino acid mother liquor and the corn steep liquor comprises the following steps:
step a: culturing yeast plates: preparing a YEPD agar culture medium, sterilizing at 121deg.C for 30min, cooling to obtain a plate culture medium, inoculating yeast to the plate culture medium in a sterile room, and culturing in a biochemical incubator at 31deg.C for 16-18 hr;
step b: preparing shake flask seed liquid: 30g of peptone, 20g of yeast extract powder, 30g of glucose, 2000g of distilled water and natural pH, sterilizing at 121 ℃ for 30min by a sterilizing pot, preparing a shake flask liquid culture medium, inoculating the prepared yeast flat plate into a shake flask in a sterile room, and delivering the shake flask liquid culture medium to shake flask for culturing at 31 ℃ for 20-22h at a shaking table rotating speed of 200r/min and an OD (10 times 600 nm) of about 1.6 after culturing to obtain shake flask seed liquid;
step c: preparing primary seed liquid: taking 1Kg of glucose, 0.1Kg of soybean meal hydrolysate, 5Kg of corn steep liquor, 2g of magnesium sulfate, 10g of ammonium sulfate and 10g of defoamer, preparing a first-stage seed culture medium after sterilizing at 121 ℃ for 60min and cooling, transferring the prepared shake flask seed liquid to a seed tank, stirring, ventilating and culturing, wherein the inoculum size is 0.1-0.2%, the culturing temperature is 30-38 ℃ and the time is 20-22h, and the OD (10 times 600 nm) is about 1.6 after culturing is finished, thus obtaining the first-stage seed liquid;
step d: industrial culture in a fermentation tank: uniformly mixing an amino acid mother solution and corn steep liquor in a ratio of 1:1 to 1:10, sterilizing by a solid elimination or continuous elimination system, pumping into a fermentation tank, cooling to 34 ℃, naturally adjusting pH, transferring the prepared primary seed solution to the fermentation tank, stirring, ventilating and culturing, wherein the inoculum size is 0.2-0.5%, the culturing temperature is 30-38 ℃, the fermentation time is 22-26h, and the fermentation end point pH value is 6.5-6.8;
step e: and (3) evaporating and concentrating: d, vacuum and low-temperature evaporation concentration is carried out on the fermentation liquor with the pH value of 6.5-6.8 produced in the step d by adopting a waste heat or MVR evaporator, and the dry matter content of the concentrated liquor is 58-65%;
step f: spray fluidized bed drying granulation: and c, drying and granulating the concentrated solution obtained in the step e by a two-fluid spray gun (air+concentrated solution) in a spray fluidized bed to obtain a feed yeast protein granule product, wherein the crude protein content is more than or equal to 53%, the water content is less than or equal to 10%, and the particle size is 0.7-1.7mm.
Example 3
In example 2, the following steps are added:
the fermentation tank used in the step d is a mechanically stirring and ventilating fermentation tank, and the amino acid mother liquor used is one or two of threonine mother liquor and lysine mother liquor and is mixed with corn steep liquor to form a fermentation culture substrate of the yeast.
The method for fermenting the feed yeast protein particles by mixing the amino acid mother liquor and the corn steep liquor comprises the following steps:
step a: culturing yeast plates: preparing a YEPD agar culture medium, sterilizing at 121deg.C for 30min, cooling to obtain a plate culture medium, inoculating yeast to the plate culture medium in a sterile room, and culturing in a biochemical incubator at 31deg.C for 16-18 hr;
step b: preparing shake flask seed liquid: 30g of peptone, 20g of yeast extract powder, 30g of glucose, 2000g of distilled water and natural pH, sterilizing at 121 ℃ for 30min by a sterilizing pot, preparing a shake flask liquid culture medium, inoculating the prepared yeast flat plate into a shake flask in a sterile room, and delivering the shake flask liquid culture medium to shake flask for culturing at 31 ℃ for 20-22h at a shaking table rotating speed of 200r/min and an OD (10 times 600 nm) of about 1.6 after culturing to obtain shake flask seed liquid;
step c: preparing primary seed liquid: taking 1Kg of glucose, 0.1Kg of soybean meal hydrolysate, 5Kg of corn steep liquor, 2g of magnesium sulfate, 10g of ammonium sulfate and 10g of defoamer, preparing a first-stage seed culture medium after sterilizing at 121 ℃ for 60min and cooling, transferring the prepared shake flask seed liquid to a seed tank, stirring, ventilating and culturing, wherein the inoculum size is 0.1-0.2%, the culturing temperature is 30-38 ℃ and the time is 20-22h, and the OD (10 times 600 nm) is about 1.6 after culturing is finished, thus obtaining the first-stage seed liquid;
step d: industrial culture in a fermentation tank: uniformly mixing an amino acid mother solution and corn steep liquor in a ratio of 1:1 to 1:10, sterilizing by a solid elimination or continuous elimination system, pumping into a fermentation tank, cooling to 34 ℃, naturally adjusting pH, transferring the prepared primary seed solution to the fermentation tank, stirring, ventilating and culturing, wherein the inoculum size is 0.2-0.5%, the culturing temperature is 30-38 ℃, the fermentation time is 22-26h, and the fermentation end point pH value is 6.5-6.8;
step e: and (3) evaporating and concentrating: d, vacuum and low-temperature evaporation concentration is carried out on the fermentation liquor with the pH value of 6.5-6.8 produced in the step d by adopting a waste heat or MVR evaporator, and the dry matter content of the concentrated liquor is 58-65%;
step f: spray fluidized bed drying granulation: and c, drying and granulating the concentrated solution obtained in the step e by a two-fluid spray gun (air+concentrated solution) in a spray fluidized bed to obtain a feed yeast protein granule product, wherein the crude protein content is more than or equal to 53%, the water content is less than or equal to 10%, and the particle size is 0.7-1.7mm.
Example 4
In example 3, the following steps are added:
and e, adopting an evaporator which is a waste heat or MVR multi-effect forced circulation evaporator to highly concentrate the fermentation liquor, wherein the dry matter content of the obtained concentrated liquor is 58-65%, and the temperature of the concentrated liquor is 65-75 ℃.
The method for fermenting the feed yeast protein particles by mixing the amino acid mother liquor and the corn steep liquor comprises the following steps:
step a: culturing yeast plates: preparing a YEPD agar culture medium, sterilizing at 121deg.C for 30min, cooling to obtain a plate culture medium, inoculating yeast to the plate culture medium in a sterile room, and culturing in a biochemical incubator at 31deg.C for 16-18 hr;
step b: preparing shake flask seed liquid: 30g of peptone, 20g of yeast extract powder, 30g of glucose, 2000g of distilled water and natural pH, sterilizing at 121 ℃ for 30min by a sterilizing pot, preparing a shake flask liquid culture medium, inoculating the prepared yeast flat plate into a shake flask in a sterile room, and delivering the shake flask liquid culture medium to shake flask for culturing at 31 ℃ for 20-22h at a shaking table rotating speed of 200r/min and an OD (10 times 600 nm) of about 1.6 after culturing to obtain shake flask seed liquid;
step c: preparing primary seed liquid: taking 1Kg of glucose, 0.1Kg of soybean meal hydrolysate, 5Kg of corn steep liquor, 2g of magnesium sulfate, 10g of ammonium sulfate and 10g of defoamer, preparing a first-stage seed culture medium after sterilizing at 121 ℃ for 60min and cooling, transferring the prepared shake flask seed liquid to a seed tank, stirring, ventilating and culturing, wherein the inoculum size is 0.1-0.2%, the culturing temperature is 30-38 ℃ and the time is 20-22h, and the OD (10 times 600 nm) is about 1.6 after culturing is finished, thus obtaining the first-stage seed liquid;
step d: industrial culture in a fermentation tank: uniformly mixing an amino acid mother solution and corn steep liquor in a ratio of 1:1 to 1:10, sterilizing by a solid elimination or continuous elimination system, pumping into a fermentation tank, cooling to 34 ℃, naturally adjusting pH, transferring the prepared primary seed solution to the fermentation tank, stirring, ventilating and culturing, wherein the inoculum size is 0.2-0.5%, the culturing temperature is 30-38 ℃, the fermentation time is 22-26h, and the fermentation end point pH value is 6.5-6.8;
step e: and (3) evaporating and concentrating: d, vacuum and low-temperature evaporation concentration is carried out on the fermentation liquor with the pH value of 6.5-6.8 produced in the step d by adopting a waste heat or MVR evaporator, and the dry matter content of the concentrated liquor is 58-65%;
step f: spray fluidized bed drying granulation: and c, drying and granulating the concentrated solution obtained in the step e by a two-fluid spray gun (air+concentrated solution) in a spray fluidized bed to obtain a feed yeast protein granule product, wherein the crude protein content is more than or equal to 53%, the water content is less than or equal to 10%, and the particle size is 0.7-1.7mm.
Example 5
In example 4, the following steps are added:
and d, the fermentation liquor crude protein at the fermentation end point in the step is more than or equal to 53%, the total amino acid sum is more than or equal to 48%, and the fermentation liquor has ester fragrance.
The method for fermenting the feed yeast protein particles by mixing the amino acid mother liquor and the corn steep liquor comprises the following steps:
step a: culturing yeast plates: preparing a YEPD agar culture medium, sterilizing at 121deg.C for 30min, cooling to obtain a plate culture medium, inoculating yeast to the plate culture medium in a sterile room, and culturing in a biochemical incubator at 31deg.C for 16-18 hr;
step b: preparing shake flask seed liquid: 30g of peptone, 20g of yeast extract powder, 30g of glucose, 2000g of distilled water and natural pH, sterilizing at 121 ℃ for 30min by a sterilizing pot, preparing a shake flask liquid culture medium, inoculating the prepared yeast flat plate into a shake flask in a sterile room, and delivering the shake flask liquid culture medium to shake flask for culturing at 31 ℃ for 20-22h at a shaking table rotating speed of 200r/min and an OD (10 times 600 nm) of about 1.6 after culturing to obtain shake flask seed liquid;
step c: preparing primary seed liquid: taking 1Kg of glucose, 0.1Kg of soybean meal hydrolysate, 5Kg of corn steep liquor, 2g of magnesium sulfate, 10g of ammonium sulfate and 10g of defoamer, preparing a first-stage seed culture medium after sterilizing at 121 ℃ for 60min and cooling, transferring the prepared shake flask seed liquid to a seed tank, stirring, ventilating and culturing, wherein the inoculum size is 0.1-0.2%, the culturing temperature is 30-38 ℃ and the time is 20-22h, and the OD (10 times 600 nm) is about 1.6 after culturing is finished, thus obtaining the first-stage seed liquid;
step d: industrial culture in a fermentation tank: uniformly mixing an amino acid mother solution and corn steep liquor in a ratio of 1:1 to 1:10, sterilizing by a solid elimination or continuous elimination system, pumping into a fermentation tank, cooling to 34 ℃, naturally adjusting pH, transferring the prepared primary seed solution to the fermentation tank, stirring, ventilating and culturing, wherein the inoculum size is 0.2-0.5%, the culturing temperature is 30-38 ℃, the fermentation time is 22-26h, and the fermentation end point pH value is 6.5-6.8;
step e: and (3) evaporating and concentrating: d, vacuum and low-temperature evaporation concentration is carried out on the fermentation liquor with the pH value of 6.5-6.8 produced in the step d by adopting a waste heat or MVR evaporator, and the dry matter content of the concentrated liquor is 58-65%;
step f: spray fluidized bed drying granulation: and c, drying and granulating the concentrated solution obtained in the step e by a two-fluid spray gun (air+concentrated solution) in a spray fluidized bed to obtain a feed yeast protein granule product, wherein the crude protein content is more than or equal to 53%, the water content is less than or equal to 10%, and the particle size is 0.7-1.7mm.
Example 6
In example 5, the following steps are added:
in the step f, a spray fluidized bed is adopted for drying and granulating, and after vibration screening, the yeast protein particles are cooled by a cooling fluidized bed to obtain the feed yeast protein particles with the diameter of 0.7-1.7mm, wherein the crude protein is more than or equal to 53%, the sum of amino acids is more than or equal to 48%, and the water content is less than or equal to 10%.
The method for fermenting the feed yeast protein particles by mixing the amino acid mother liquor and the corn steep liquor comprises the following steps:
step a: culturing yeast plates: preparing a YEPD agar culture medium, sterilizing at 121deg.C for 30min, cooling to obtain a plate culture medium, inoculating yeast to the plate culture medium in a sterile room, and culturing in a biochemical incubator at 31deg.C for 16-18 hr;
step b: preparing shake flask seed liquid: 30g of peptone, 20g of yeast extract powder, 30g of glucose, 2000g of distilled water and natural pH, sterilizing at 121 ℃ for 30min by a sterilizing pot, preparing a shake flask liquid culture medium, inoculating the prepared yeast flat plate into a shake flask in a sterile room, and delivering the shake flask liquid culture medium to shake flask for culturing at 31 ℃ for 20-22h at a shaking table rotating speed of 200r/min and an OD (10 times 600 nm) of about 1.6 after culturing to obtain shake flask seed liquid;
step c: preparing primary seed liquid: taking 1Kg of glucose, 0.1Kg of soybean meal hydrolysate, 5Kg of corn steep liquor, 2g of magnesium sulfate, 10g of ammonium sulfate and 10g of defoamer, preparing a first-stage seed culture medium after sterilizing at 121 ℃ for 60min and cooling, transferring the prepared shake flask seed liquid to a seed tank, stirring, ventilating and culturing, wherein the inoculum size is 0.1-0.2%, the culturing temperature is 30-38 ℃ and the time is 20-22h, and the OD (10 times 600 nm) is about 1.6 after culturing is finished, thus obtaining the first-stage seed liquid;
step d: industrial culture in a fermentation tank: uniformly mixing an amino acid mother solution and corn steep liquor in a ratio of 1:1 to 1:10, sterilizing by a solid elimination or continuous elimination system, pumping into a fermentation tank, cooling to 34 ℃, naturally adjusting pH, transferring the prepared primary seed solution to the fermentation tank, stirring, ventilating and culturing, wherein the inoculum size is 0.2-0.5%, the culturing temperature is 30-38 ℃, the fermentation time is 22-26h, and the fermentation end point pH value is 6.5-6.8;
step e: and (3) evaporating and concentrating: d, vacuum and low-temperature evaporation concentration is carried out on the fermentation liquor with the pH value of 6.5-6.8 produced in the step d by adopting a waste heat or MVR evaporator, and the dry matter content of the concentrated liquor is 58-65%;
step f: spray fluidized bed drying granulation: and c, drying and granulating the concentrated solution obtained in the step e by a two-fluid spray gun (air+concentrated solution) in a spray fluidized bed to obtain a feed yeast protein granule product, wherein the crude protein content is more than or equal to 53%, the water content is less than or equal to 10%, and the particle size is 0.7-1.7mm.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The method for mixed fermentation of the feed yeast protein particles by the amino acid mother liquor and the corn steep liquor is characterized by comprising the following steps: the method comprises the following steps:
step a: culturing yeast plates: preparing a YEPD agar culture medium, sterilizing at 121deg.C for 30min, cooling to obtain a plate culture medium, inoculating yeast to the plate culture medium in a sterile room, and culturing in a biochemical incubator at 31deg.C for 16-18 hr;
step b: preparing shake flask seed liquid: 30g of peptone, 20g of yeast extract powder, 30g of glucose, 2000g of distilled water and natural pH, sterilizing at 121 ℃ for 30min by a sterilizing pot, preparing a shake flask liquid culture medium, inoculating the prepared yeast flat plate into a shake flask in a sterile room, and delivering the shake flask liquid culture medium to shake flask for culturing at 31 ℃ for 20-22h at a shaking table rotating speed of 200r/min and an OD (10 times 600 nm) of about 1.6 after culturing to obtain shake flask seed liquid;
step c: preparing primary seed liquid: taking 1Kg of glucose, 0.1Kg of soybean meal hydrolysate, 5Kg of corn steep liquor, 2g of magnesium sulfate, 10g of ammonium sulfate and 10g of defoamer, preparing a first-stage seed culture medium after sterilizing at 121 ℃ for 60min and cooling, transferring the prepared shake flask seed liquid to a seed tank, stirring, ventilating and culturing, wherein the inoculum size is 0.1-0.2%, the culturing temperature is 30-38 ℃ and the time is 20-22h, and the OD (10 times 600 nm) is about 1.6 after culturing is finished, thus obtaining the first-stage seed liquid;
step d: industrial culture in a fermentation tank: uniformly mixing an amino acid mother solution and corn steep liquor in a ratio of 1:1 to 1:10, sterilizing by a solid elimination or continuous elimination system, pumping into a fermentation tank, cooling to 34 ℃, naturally adjusting pH, transferring the prepared primary seed solution to the fermentation tank, stirring, ventilating and culturing, wherein the inoculum size is 0.2-0.5%, the culturing temperature is 30-38 ℃, the fermentation time is 22-26h, and the fermentation end point pH value is 6.5-6.8;
step e: and (3) evaporating and concentrating: d, vacuum and low-temperature evaporation concentration is carried out on the fermentation liquor with the pH value of 6.5-6.8 produced in the step d by adopting a waste heat or MVR evaporator, and the dry matter content of the concentrated liquor is 58-65%;
step f: spray fluidized bed drying granulation: and c, drying and granulating the concentrated solution obtained in the step e by a two-fluid spray gun (air+concentrated solution) in a spray fluidized bed to obtain a feed yeast protein granule product, wherein the crude protein content is more than or equal to 53%, the water content is less than or equal to 10%, and the particle size is 0.7-1.7mm.
2. The method for mixed fermentation of feed yeast protein particles with amino acid mother liquor and corn steep liquor according to claim 1, wherein the method comprises the following steps: the yeast is one or two selected from candida, kluyveromyces and isaccharomyces.
3. The method for mixed fermentation of feed yeast protein particles with amino acid mother liquor and corn steep liquor according to claim 1, wherein the method comprises the following steps: the fermentation tank used in the step d is a mechanically stirring and ventilating fermentation tank, and the used amino acid mother liquor is one or two of threonine mother liquor and lysine mother liquor and is mixed with corn steep liquor to form a fermentation culture substrate of yeast.
4. The method for mixed fermentation of feed yeast protein particles with amino acid mother liquor and corn steep liquor according to claim 1, wherein the method comprises the following steps: and e, the adopted evaporator is a waste heat or MVR multi-effect forced circulation evaporator, the fermentation liquor is highly concentrated, the dry matter content of the obtained concentrated liquor is 58-65%, and the temperature of the concentrated liquor is 65-75 ℃.
5. The method for mixed fermentation of feed yeast protein particles with amino acid mother liquor and corn steep liquor according to claim 1, wherein the method comprises the following steps: and d, the fermentation liquor crude protein at the fermentation end point in the step is more than or equal to 53%, the total amino acid is more than or equal to 48%, and the fermentation liquor crude protein has ester fragrance.
6. The method for mixed fermentation of feed yeast protein particles with amino acid mother liquor and corn steep liquor according to claim 1, wherein the method comprises the following steps: in the step f, a spray fluidized bed is adopted for drying and granulating, and after vibration screening, the yeast protein particles are cooled by a cooling fluidized bed to obtain the feed yeast protein particles with the diameter of 0.7-1.7mm, wherein the crude protein is more than or equal to 53%, the total amino acid is more than or equal to 48%, and the water content is less than or equal to 10%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310600661.7A CN116584576A (en) | 2023-05-25 | 2023-05-25 | Method for mixed fermentation of feed yeast protein particles by amino acid mother liquor and corn steep liquor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310600661.7A CN116584576A (en) | 2023-05-25 | 2023-05-25 | Method for mixed fermentation of feed yeast protein particles by amino acid mother liquor and corn steep liquor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116584576A true CN116584576A (en) | 2023-08-15 |
Family
ID=87607854
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310600661.7A Pending CN116584576A (en) | 2023-05-25 | 2023-05-25 | Method for mixed fermentation of feed yeast protein particles by amino acid mother liquor and corn steep liquor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116584576A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6238728B1 (en) * | 1999-05-28 | 2001-05-29 | Ajinomoto Co., Inc. | Mixed feed with an amino acid |
| CN1449673A (en) * | 2002-11-07 | 2003-10-22 | 吉林省环宇饲料有限公司 | Single cell protein fodder and preparation process thereof |
| CN101709272A (en) * | 2009-11-26 | 2010-05-19 | 秦皇岛骊骅淀粉股份有限公司 | Method for preparing feed yeast |
| CN104726335A (en) * | 2015-04-13 | 2015-06-24 | 呼伦贝尔东北阜丰生物科技有限公司 | Method for manufacturing granule proteins by aid of waste mother liquid of lysine |
| CN109305831A (en) * | 2018-12-03 | 2019-02-05 | 寿光金远东变性淀粉有限公司 | A method of biological organic fertilizer is made using corn pulp and fermentation waste liquid of lysine |
| CN109793097A (en) * | 2018-12-26 | 2019-05-24 | 河南巨龙生物工程股份有限公司 | A kind of fermentation process using sugaring tailing and tryptophan mother liquor production biological feedstuff |
| CN113080307A (en) * | 2021-04-14 | 2021-07-09 | 廊坊梅花生物技术开发有限公司 | Method for producing feed by using amino acid fermentation mother liquor and corn sugar residues |
| CN113455584A (en) * | 2021-07-28 | 2021-10-01 | 山西长清生物科技有限公司 | Method for producing feed yeast particles by using corn steep liquor |
-
2023
- 2023-05-25 CN CN202310600661.7A patent/CN116584576A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6238728B1 (en) * | 1999-05-28 | 2001-05-29 | Ajinomoto Co., Inc. | Mixed feed with an amino acid |
| CN1449673A (en) * | 2002-11-07 | 2003-10-22 | 吉林省环宇饲料有限公司 | Single cell protein fodder and preparation process thereof |
| CN101709272A (en) * | 2009-11-26 | 2010-05-19 | 秦皇岛骊骅淀粉股份有限公司 | Method for preparing feed yeast |
| CN104726335A (en) * | 2015-04-13 | 2015-06-24 | 呼伦贝尔东北阜丰生物科技有限公司 | Method for manufacturing granule proteins by aid of waste mother liquid of lysine |
| CN109305831A (en) * | 2018-12-03 | 2019-02-05 | 寿光金远东变性淀粉有限公司 | A method of biological organic fertilizer is made using corn pulp and fermentation waste liquid of lysine |
| CN109793097A (en) * | 2018-12-26 | 2019-05-24 | 河南巨龙生物工程股份有限公司 | A kind of fermentation process using sugaring tailing and tryptophan mother liquor production biological feedstuff |
| CN113080307A (en) * | 2021-04-14 | 2021-07-09 | 廊坊梅花生物技术开发有限公司 | Method for producing feed by using amino acid fermentation mother liquor and corn sugar residues |
| CN113455584A (en) * | 2021-07-28 | 2021-10-01 | 山西长清生物科技有限公司 | Method for producing feed yeast particles by using corn steep liquor |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101709272B (en) | Method for preparing feed yeast | |
| US20150191754A1 (en) | Method for preparing short-chain fatty acid having high propanoic acid content by continuous fermentation | |
| CN109504720B (en) | Green production process of glutamic acid | |
| CN101457242A (en) | Novel process for greenly and high efficiently improving L-glutamic acid fermentation production rate | |
| CN101747093A (en) | Method for producing mycoprotein and polypeptide organic fertilizer by fermented waste of vitamin C | |
| CN104844468A (en) | Environment-friendly technology for treating threonine fermentation mother liquid | |
| CN109439702A (en) | The technique for handling threonine high gravity fermentation waste water | |
| CN110384178B (en) | Lactic acid bacteria culture prepared based on vinasse and application of lactic acid bacteria culture in animal feed | |
| CN105085023A (en) | Foliar fertilizer prepared through Chinese medicine residue fermentation and preparation method thereof | |
| CN102987063B (en) | Organic acid animal growth regulator and preparation method thereof | |
| CN1171539C (en) | Nutrients additive for biological feed of livestock and fowls and its preparing process | |
| CN113817635A (en) | Method for culturing bacillus by using soybean whey wastewater | |
| CN109607823A (en) | The method for administering threonine mother liquor and separation and Extraction albumen | |
| CN1912102A (en) | Method for producing fodde yeast using corn soaking water | |
| CN106244638B (en) | Comprehensive utilization process for producing lactic acid by biomass circulating fermentation | |
| CN105152805A (en) | Mineral foliar fertilizer suitable for fruit trees and preparation method of fertilizer | |
| CN106086093B (en) | Lactic acid fermentation bacteria residue pretreatment method and method for producing lactic acid by circular fermentation | |
| CN105110899A (en) | Humic acid foliar fertilizer and preparation method therefor | |
| CN1799363A (en) | Method for preparing bacillus thuringiensis microbiological pesticide by starch waste liquor | |
| CN104313105A (en) | Method for preparing 65% threonine by using threonine fermenting liquor and waste mother liquor | |
| CN101054558A (en) | Method for preparing feedstuff yeast from maize peel hydrolysis sugar solution after extracting xylose | |
| CN114958631B (en) | Method for producing single cell protein by using heavy phase lactic acid | |
| CN113455584B (en) | Method for producing feed yeast particles by using corn steep liquor | |
| CN116584576A (en) | Method for mixed fermentation of feed yeast protein particles by amino acid mother liquor and corn steep liquor | |
| CN105152710A (en) | Powdery foliar fertilizer prepared through milk fermentation and preparation method of powdery foliar fertilizer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |