CN101709272B - Method for preparing feed yeast - Google Patents
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Abstract
The invention discloses a method for preparing feed yeast. The method comprises the following steps of: using waste solution obtained after phytin is extracted from water in which corn is soaked as a yeast culture substrate, using liquid ammonia and albumens as nitrogen sources, using mother solution of glucose, liquid glucose or other molasses as carbon sources, mixing the raw materials in a ratio, adding trace elements to the mixture to form a culture medium, adjusting the pH value and temperature of the culture medium, adding bacteria in a self priming fermentor, performing expansion culture for several times till the requirement for mass production is met, and finally obtaining the finished products of the feed yeast by evaporating concentration and centrifugal atomization drying. The method solves the bottleneck that the phytin waste solution and mother solution, which are generated during the production of inositol, cannot be effectively treated, so that the development is restricted and enforces the development of the inositol industry; and the method also has the advantages of maximizing the resource utilization, changing the wastes into the valuables, protecting the environment and increasing the added value of the products.
Description
Technical field
The invention belongs to the microbial fermentation technology field, be specifically related to a kind of method that waste liquid is produced fodder yeast behind the luxuriant and rich with fragrance spit of fland of corn soaking water extraction of utilizing.
Background technology
The fodder yeast rich in protein, amino acid is complete, and proportioning is formed rationally; And contain and enrich vitamin B group, mineral element, trace element, enzyme, glucide, physiologically active substance and positive growth factor; Be a kind of quality protein that is of high nutritive value and can substituted fish meal, it has nutrition, food calling and the probiotic bacterium multiple function of rising in value, and promotes livestock and poultry metabolism; Strengthen the livestock and poultry resistance against diseases, improve growth of animals or poultry speed, prolificacy, meat and fur quality.Being widely used in livestock and poultry breeding industry, is the best substitute of fish meal, can reduce the cost of aquaculture, has great economic benefit and social benefit.
But the domestic producer that utilizes liquid submerged fermentation method to produce fodder yeast is in end-of-life state basically, and fodder yeast is the yeast feed that adopts solid fermentation more than 95% on the market, and its nutritive value does not reach the standard of fodder yeast far away.Its reason is exactly the serious backwardness that yeast is produced zymotechnique.The domestic production fodder yeast mainly adopted airlift fermentor, bubbling fermentation tank and injection self-suction fermentor in the past, and air lift type and bubbling fermentation tank fermentation period are long, and energy consumption is quite big, and cost is high; It is low to spray self-suction fermentor later stage dissolved oxygen coefficient, and thalli growth is restricted, and quality product is bad.
At present the domestic production inositol mainly be sub product soak water of maize with W-Gum processing as raw material, from soak water of maize, extract luxuriant and rich with fragrance spit of fland earlier, luxuriant and rich with fragrance spit of fland pyrohydrolysis is produced inositol.But generation luxuriant and rich with fragrance spit of fland waste liquid and inositol mother liquor cause very big pollution to environment in producing the inositol process.At present domestic also do not have method to handle to the inositol mother liquor, is to mix with soak water of maize to luxuriant and rich with fragrance spit of fland method for treating waste liquid one, is added to after the evaporation concentration in the fibrous residue; The 2nd, direct discharging.First method has reduced the utilization ratio of soak water of maize, vaporizer easy scale formation, necessary low-temperature evaporation; Energy consumption is big, influences the fibrous residue palatability, and the present domestic sewage treatment process of second method has been handled not; Can only directly discharge, serious environment pollution causes the wasting of resources simultaneously.This is seriously restricting the development of corn deep processing industries such as inositol.
Present problem is: 1, adopts maize peel hydrolysis filtrating to do carbon source, also needs chromatographic separation and handle from handing over through the filtrating of high temperature, acid hydrolysis, and the technology very complicated, and also the filter residue that produces needs to handle.Hydrolysis sugar liquid hammer degree is very low in addition, needs fermentor tank quantity many, causes plant factor low, and production cost is very high.2, directly utilize soak water of maize to be raw material,, cause that fermented liquid deposition is many, the cycle is grown, the thalline number is not full less because soak water of maize impurity is many, ash content is high; Have a strong impact on fermentation culture, and in spray-drying process, atomizing effect is poor; Product lumps easily and becomes to stick together, and color is dark, and ash content is more than 25%; Belong to substandard product, it is feeding to be not suitable for animal.3, adopt sodium hydroxide to regulate substratum ph value, do not reach the effect of this technology, but also can increase the product ash content.4, sugar content 2-6% at the bottom of the fermentor tank.5, adopt stream to add acid control pH value, SO42-or cl-content are high in the product, to the poisonous secondary harm of animal; If the pH value is lower than 4, fermentation period obviously prolongs, and is unfavorable for fermentation culture.6, utilizing behind the luxuriant and rich with fragrance spit of fland of corn soaking water extraction waste liquid and Fei Ting pyrohydrolysis to produce inositol produces mother liquor and handles problems and can't be resolved.Therefore, utilize that waste liquid is the developing direction in this field with inositol mother liquor production fodder yeast behind the luxuriant and rich with fragrance spit of fland of corn soaking water extraction.Not only improve the soak water of maize added value, and solved the liquid waste disposal problem in the production inositol process, realized the maximization of the cleaner production and the utilization of resources.
Summary of the invention
In view of above-mentioned present situation; The invention provides a kind of preparation method of fodder yeast; Produce waste liquid and inositol mother liquor production fodder yeast after utilizing the luxuriant and rich with fragrance spit of fland of corn soaking water extraction,, reduced fermentation time and production cost through the self-suction fermentor optimization for fermentation technology; Improve dissolved oxygen coefficient, realize the utilization of cleaner production and resource.
Preparing method of the present invention comprises the steps:
The preparation substratum
(1) at first chooses the glucose mother liquid of concentration 42-50%, reducing sugar content >=80%;
(2) with waste liquid behind the luxuriant and rich with fragrance spit of fland of corn soaking water extraction, control pH value 5.8-6.0 is through reducing ash content and pH value wherein after the IX;
(3) waste liquid behind glucose mother liquid and the luxuriant and rich with fragrance spit of fland of corn soaking water extraction is mixed, control end sugar concentration 10-15% regulates pH value 4-6 with ammoniacal liquor, and it is subsequent use to process the fodder yeast substratum;
The preparation seed liquor
(4) get yeast and after the three grades of cultivations in inclined-plane, carry out the shaking table cultivation, culture temperature 28-32 ℃, time 10-14 hour, cultivate and finish residual sugar≤0.5%, OD value 1.20-1.35 obtains the shaking table seed liquor;
(5) the shaking table seed liquor is inoculated into fermentor tank, through the secondary enlarged culturing, culture condition: sugared content 2-4%, inoculum size 8-10%, seeding tank liquid amount 70% incubation time 8-10 hour, obtains secondary and cultivates seed liquor;
Ferment tank
(6) the fodder yeast substratum is squeezed in the self-suction fermentor with pump; (its end sugar content 2-4%, temperature 28-32 ℃; )
(7) getting secondary cultivates seed liquor and is inoculated in the self-suction fermentor and ferments inoculum size 8-10%, control end sugar content 1.5-3%; Temperature 28-32 ℃, ventilating ratio 1: 0.5-1, fermentation period 8-12 hour; Cultivate and finish OD value 1.6-1.8, cultivate and finish residual sugar≤0.5%, obtain fermented liquid;
Evaporation concentration
(8) adopt waste heat evaporator that fermented liquid is carried out evaporation concentration, steam secondary fermentation liquid concentration 15-40%;
Spraying drying
(9) will steam secondary fermentation liquid and carry out spraying drying, promptly obtain fodder yeast through the hig h-speed centrifugal spray drying tower.
In the present invention, the barms of indication is be selected from Candida utilis, candida tropicalis or cereuisiae fermentum a kind of.
Further, the fodder yeast substratum selects for use behind the luxuriant and rich with fragrance spit of fland of corn soaking water extraction waste liquid to extract behind the luxuriant and rich with fragrance spit of fland waste liquid directly or add glucose mother liquid or liquid sugar production fodder yeast substratum for utilizing soak water of maize or rice bran.
Further, waste liquid is through reducing ash content and ph value wherein after IX or the electrodialysis behind the luxuriant and rich with fragrance spit of fland of said corn soaking water extraction.
Further, the yeast culture base is cultivated and is finished fermented liquid bacterium inspection 15-30 hundred million/milliliter; Hundred million/gram of yeast product bacterium amount 150-300, crude protein>=45%, ash content≤10%, heavy metal (in Pb)≤10mg/kg.
Further, granularity keeps yeast product moisture 6-8% through the SSW0.400/0.250mm testing sieve after the fermented liquid drying.
Further, select waste liquid friendship back liquid mixing behind the luxuriant and rich with fragrance spit of fland of glucose mother liquid and corn soaking water extraction for use; Or waste liquid hands over back liquid to mix after selecting the luxuriant and rich with fragrance spit of fland of glucose mother liquid, inositol mother liquor and corn soaking water extraction for use.
In sum; Positively effect of the present invention is: 1 is raw material with waste liquid, glucose mother liquid and inositol mother liquor behind the luxuriant and rich with fragrance spit of fland of corn soaking water extraction; Be corn deep processing by product and waste; Through fermentation, concentrate, obtain product after the drying, have that operational path is short, the characteristics of instant effect, this is peerless.2 adopt treatment on ion exchange columns to extract waste liquid behind the luxuriant and rich with fragrance spit of fland, hand over back liquid clear, can reach unified specification, can effectively reduce the ash content in the raw material, and the product ash content is below 10%, heavy metal (in Pb)≤10mg/kg.3 use ammoniacal liquor to regulate the medium pH value, make bacterial classification not only absorb the organonitrogen in the substratum, also make full use of the inorganic nitrogen in the ammoniacal liquor; Improve growth speed greatly; Fermentation period is in 8 hours, and the thalline number is more than 18 hundred million/ml, and product thalline number reaches more than 18,000,000,000/gram.(4) sugar content 1.5-3% at the bottom of this technology fermentor tank, thus the glucose mother liquid consumption saved, reduce production costs.(5) this technology need not flow and add acid control pH value, and normal cultured gets final product.(6) utilize soak water of maize and fibrous residue hydrolyzed solution, crude protein, glucose mother liquid and inositol mother liquor to produce fodder yeast; Through the analysis-by-synthesis of aspects such as production cost, economic benefit, environmental pollution, energy-saving and emission-reduction, confirm to utilize waste liquid behind the luxuriant and rich with fragrance spit of fland of corn soaking water extraction, glucose mother liquid or and the luxuriant and rich with fragrance spit of fland of corn soaking water extraction after waste liquid, glucose mother liquid and inositol mother liquor produce fodder yeast and be preferred plan.
In addition, the self-suction fermentor optimization for fermentation technology reduces fermentation time, improves dissolved oxygen coefficient, has reduced production cost, makes the fodder yeast cost have market competition advantage.Simultaneously, guaranteeing that incubation time is constant, under the prerequisite that quality product does not reduce, will cultivate concentration has increased more than one times, greatly reduces production cost, has solved and has produced the high difficult problem of fodder yeast cost.In addition, waste liquid made full use of the nutritive substances such as soluble amino acid that wherein contain as the substratum of producing fodder yeast after the present invention will utilize the luxuriant and rich with fragrance spit of fland of corn soaking water extraction, produced fodder yeast.Solving on the basis of environmental issue like this, can realize that cleaning reduces discharging to produce, again through self-suction fermentor during the fermentation the dissolved oxygen utilization ratio can reach 50%; Producing the required air capacity of per kilogram yeast solid substance approximately is the 25-30% of bubbling fermentation tank, has high dissolved oxygen, energy-conservation characteristics.
Embodiment
Below in conjunction with embodiment the present invention is described further.
Embodiment one
The preparation method of fodder yeast, step is following:
According to the growth characteristics of candida tropicalis, growth factors such as the nitrogenous source in the main dependence of the candida tropicalis growth substratum, carbon source, inorganic salt, trace element, these nutritive substances mainly rely on the batching substratum to provide.Comprise:
One, medium preparation
1, glucose mother liquid is a sub product of producing glucose, gets the glucose mother liquid of concentration 42%, and the glucose mother liquid of reducing sugar content >=80% is subsequent use;
2, waste liquid clear behind the luxuriant and rich with fragrance spit of fland of corn soaking water extraction, control pH value 5.8, in order to reduce ash content and pH value wherein, luxuriant and rich with fragrance spit of fland waste liquid will pass through IX, and luxuriant and rich with fragrance spit of fland waste liquid hands over back liquid subsequent use;
3, hand over back liquid to mix waste liquid behind glucose mother liquid and the luxuriant and rich with fragrance spit of fland of corn soaking water extraction, controlling concn 10% is regulated pH value 4 with ammoniacal liquor, and sterilization is 30 minutes under 130 ℃ of conditions, and is subsequent use as the candida tropicalis bacterium culture medium;
Two, preparation seed liquor
4, the candida tropicalis bacterium is carried out shaking table after the three grades of cultivations in inclined-plane and cultivate, 28 ℃ of culture temperature, 10 hours time, to cultivate and finish residual sugar≤0.5%, OD value 1.35 gets one-level and cultivates seed liquor;
5, the shaking table seed liquor is inoculated into fermentor tank, through the secondary enlarged culturing, culture condition: sugared content 4%, inoculum size 8-10%, seeding tank liquid amount 70%, incubation time 8 hours gets secondary and cultivates seed liquor;
Three, fermentor cultivation
6, the substratum of preparation is squeezed in the self-suction fermentor sugared content 2%, 28 ℃ of temperature with pump;
7, the secondary of preparation is cultivated seed liquor and be inoculated in the self-suction fermentor, inoculum size 8%, ventilating begins to cultivate;
8, processing condition are: dissolved oxygen>=15%, and fermentation period 14 hours is cultivated and is finished OD value 1.68, cultivates and finishes residual sugar≤0.5%;
Four, evaporation concentration
9, adopt waste heat evaporator that liquid is accomplished in fermentation and carry out evaporation concentration, the fermented liquid concentration 25% after the evaporation;
Five, spraying drying
10, steam secondary fermentation liquid and carry out spraying drying, obtain the fodder yeast product through the hig h-speed centrifugal spray drying tower.
The crude protein content of the fodder yeast product that the present invention produces is more than 45%, and several 150 of thalline/more than the gram, moisture content is below 8%.
Embodiment two
The step of producing fodder yeast is following:
One, medium preparation
According to the growth characteristics of Candida utilis, growth factors such as the nitrogenous source in the main dependence of the Candida utilis growth substratum, carbon source, inorganic salt, trace element, these nutritive substances mainly rely on the batching substratum to provide.Prepare as follows:
1, glucose mother liquid is a sub product of producing glucose, gets concentration 42%, and the glucose mother liquid of reducing sugar content >=80% is subsequent use;
2, waste liquid clear behind the luxuriant and rich with fragrance spit of fland of corn soaking water extraction, ph5.8, in order to reduce ash content and pH value wherein, waste liquid will pass through IX behind the luxuriant and rich with fragrance spit of fland of corn soaking water extraction, hands over back liquid subsequent use;
3, liquid mixes after glucose mother liquid and the friendship, and controlling concn 10% is regulated pH4 with ammoniacal liquor, and sterilization is 30 minutes under 130 ℃ of conditions, and is subsequent use as the Candida utilis bacterium culture medium;
Two, preparation seed liquor
4, the Candida utilis bacterium is carried out shaking table after the three grades of cultivations in inclined-plane and cultivate, 28 ℃ of culture temperature, 10 hours time, to cultivate and finish residual sugar≤0.5%, OD value 1.20 gets one-level and cultivates seed liquor;
5, the shaking table seed liquor is inoculated into fermentor tank, through the secondary enlarged culturing, and culture condition: sugared content 4%, inoculum size 8%, seeding tank liquid amount 70%, incubation time 8 hours gets secondary and cultivates seed liquor;
Three, fermentor cultivation
6, the substratum of preparation is squeezed in the self-suction fermentor sugared content 4%, 28 ℃ of temperature with pump;
7, the secondary of preparation is cultivated seed liquor and be inoculated in the self-suction fermentor, inoculum size 8%, ventilating begins to cultivate;
8, processing condition are: dissolved oxygen>=15%, and fermentation period 14 hours is cultivated and is finished OD value 1.68, cultivates and finishes residual sugar≤0.5%;
Four, evaporation concentration
9, adopt waste heat evaporator that liquid is accomplished in fermentation and carry out evaporation concentration, the fermented liquid concentration 25% after the evaporation;
Five, spraying drying
10, steam secondary fermentation liquid and carry out spraying drying, obtain the fodder yeast product through the hig h-speed centrifugal spray drying tower.Fodder yeast product crude protein content is more than 45%, and several 150 of thalline/more than the gram, moisture content is below 8%.
Embodiment three
The step of producing fodder yeast is following:
According to the growth characteristics of cereuisiae fermentum, growth factors such as the nitrogenous source in the main dependence of the cereuisiae fermentum growth substratum, carbon source, inorganic salt, trace element, these nutritive substances mainly rely on the batching substratum to provide.Prepare as follows:
One, medium preparation
1, glucose mother liquid is a sub product of producing glucose, gets concentration 42%, and the glucose mother liquid of reducing sugar content >=80% is subsequent use;
2, get waste liquid clear behind the luxuriant and rich with fragrance spit of fland of corn soaking water extraction, pH value 5.8, in order to reduce ash content and pH value wherein, waste liquid will pass through IX behind the luxuriant and rich with fragrance spit of fland of corn soaking water extraction, hands over back liquid subsequent use;
3, liquid mixes after glucose mother liquid and the friendship, and controlling concn 10% is regulated pH4 with ammoniacal liquor, and sterilization is 30 minutes under 120 ℃ of conditions, and is subsequent use as the cereuisiae fermentum bacterium culture medium;
Two, preparation seed liquor
4, saccharomyces cerevisiae is carried out shaking table after the three grades of cultivations in inclined-plane and cultivate, 28 ℃ of culture temperature, are cultivated and are finished residual sugar≤0.5%, OD value 1.20 at 10 hours time;
5, the shaking table seed liquor is inoculated into fermentor tank, through the secondary enlarged culturing, and culture condition: sugared content 4%, inoculum size 8%, seeding tank liquid amount 70%, incubation time 8 hours gets secondary and cultivates seed liquor;
Three, fermentor cultivation
6, the substratum of preparation is squeezed in the self-suction fermentor its sugared content 4%, 28 ℃ of temperature with pump;
7, the secondary of preparation is cultivated seed liquor and be inoculated in the self-suction fermentor, inoculum size 8%, ventilating begins to cultivate;
8, processing condition are: dissolved oxygen>=15%, and fermentation period 14 hours is cultivated and is finished OD value 1.68, cultivates and finishes residual sugar≤0.5%;
Four, evaporation concentration
9, adopt waste heat evaporator or other common vaporizers that liquid is accomplished in fermentation and carry out evaporation concentration, the fermented liquid concentration 25% after the evaporation;
Five, spraying drying
10, steam secondary fermentation liquid and carry out spraying drying, obtain the fodder yeast product through the hig h-speed centrifugal spray drying tower.
The crude protein content of the fodder yeast product that the present invention produces is more than 45%, and several 150 of thalline/more than the gram, moisture content is below 8%.
Embodiment four
According to the growth characteristics of cereuisiae fermentum, growth factors such as the nitrogenous source in the main dependence of the cereuisiae fermentum growth substratum, carbon source, inorganic salt, trace element, these nutritive substances mainly rely on the batching substratum to provide.Prepare as follows:
One, medium preparation
1, glucose mother liquid is a sub product of producing glucose, gets concentration 42%, and the glucose mother liquid of reducing sugar content >=80% is subsequent use;
2, get waste liquid clear behind the luxuriant and rich with fragrance spit of fland of corn soaking water extraction, pH value 5.8, in order to reduce ash content and pH value wherein, waste liquid will pass through IX behind the luxuriant and rich with fragrance spit of fland of corn soaking water extraction, hands over back liquid subsequent use;
3, waste liquid hands over back liquid to mix behind the luxuriant and rich with fragrance spit of fland of glucose mother liquid, inositol mother liquor and corn soaking water extraction, and controlling concn 10% is regulated pH4 with ammoniacal liquor, and sterilization is 30 minutes under 120 ℃ of conditions, and is subsequent use as the cereuisiae fermentum bacterium culture medium;
Other step is same as the previously described embodiments, the Therefore, omited.
The present invention is not limited to above-mentioned specific embodiment, and anyone can draw other various forms of variations under enlightenment of the present invention, no matter but make which kind of alternative and change, the every and same or analogous technical scheme of the application is all in protection scope of the present invention.
Claims (4)
1. the preparation method of a fodder yeast comprises:
(1) preparation substratum
Get glucose mother liquid concentration 42-50%, reducing sugar content >=80%;
With waste liquid clear behind the luxuriant and rich with fragrance spit of fland of corn soaking water extraction, ph5.8-6.0 is through reducing ash content and ph value wherein after the IX;
Hand over back liquid to mix waste liquid behind glucose mother liquid and the luxuriant and rich with fragrance spit of fland of corn soaking water extraction, control end sugar concentration 1.5-3.0% regulates pH4-6 with ammoniacal liquor, and is subsequent use as the fodder yeast substratum;
(2) preparation seed liquor
Choose yeast and behind slant culture, carry out the shaking table cultivation, culture temperature 28-32 ℃, time 10-14 hour, cultivate and finish residual sugar≤0.5%, OD value 1.20-1.35 gets one-level and cultivates seed liquor;
Shaker fermentation liquid is inoculated into fermentor tank, through the secondary enlarged culturing, culture condition: sugared content 1.5-3.0%, inoculum size 10-20%, seeding tank liquid amount 70% incubation time 8-10 hour, gets secondary and cultivates seed liquor;
(3) ferment tank
The yeast culture base of preparation is squeezed in the self-suction fermentor with pump;
The secondary of preparation is cultivated seed liquor be inoculated in the self-suction fermentor inoculum size 10-20%;
Processing condition are: control end sugar content 1.5-3%, and temperature 28-32 ℃, ventilating ratio 1: 0.5-1, fermentation period 8-12 hour, cultivate and finish OD value 1.6-1.8, cultivate and finish residual sugar≤0.5%;
(4) evaporation concentration
Adopt waste heat evaporator or triple-effect evaporator that liquid is accomplished in fermentation and carry out evaporation concentration, steam secondary fermentation liquid concentration 15-40%;
(5) spraying drying
To steam secondary fermentation liquid and carry out spraying drying, obtain fodder yeast through the hig h-speed centrifugal spray drying tower.
2. the preparation method of fodder yeast according to claim 1 is characterized in that, said barms is selected from a kind of of Candida utilis, candida tropicalis or cereuisiae fermentum.
3. the preparation method of fodder yeast according to claim 1 is characterized in that, the yeast culture base is cultivated and finished fermented liquid bacterium inspection 15-30 hundred million/milliliter; Hundred million/gram of yeast product bacterium amount 150-300, crude protein>=45%, ash content≤10%, heavy metal (in Pb)≤10mg/kg.
4. the preparation method of fodder yeast according to claim 1 is characterized in that, granularity keeps yeast product moisture 6-8% through the SSW0.400/0.250mm testing sieve after the fermented liquid drying.
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Families Citing this family (10)
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| CN101880634A (en) * | 2010-06-11 | 2010-11-10 | 秦皇岛骊骅淀粉股份有限公司 | Method for producing feed yeast by using corn steep liquor |
| CN101919488B (en) * | 2010-09-21 | 2012-08-15 | 哈尔滨工业大学 | Method for producing bioactive feed by utilizing yellow seriflux and waste molasses through fermentation |
| CN101919489B (en) * | 2010-09-21 | 2012-10-03 | 哈尔滨工业大学 | Method for producing high protein feed by utilizing yellow seriflux and waste molasses through fermentation |
| CN102321546B (en) * | 2011-09-15 | 2013-09-25 | 西北农林科技大学 | Fermentation process of top-quality yeast |
| CN102702250A (en) * | 2012-05-23 | 2012-10-03 | 山东省鲁洲食品集团有限公司 | Production method for extracting phytine from corn steep liquor to realize fodder yeast co-production |
| CN109351390B (en) * | 2016-11-30 | 2020-09-04 | 宁夏鑫德粮油工贸有限公司 | Safe and efficient echelon wheat wetting process |
| CN109694164B (en) * | 2019-02-24 | 2019-12-13 | 内蒙古拜克生物有限公司 | Recovery processing method of amino acid mother liquor |
| CN110150468A (en) * | 2019-04-30 | 2019-08-23 | 海南久纳生物科技开发有限公司 | A kind of dedicated production technology of herbage biological fermentation feed |
| CN113455584B (en) * | 2021-07-28 | 2022-06-07 | 山西长清生物科技有限公司 | Method for producing feed yeast particles by using corn steep liquor |
| CN116584576A (en) * | 2023-05-25 | 2023-08-15 | 寿光玖鼎生物科技有限公司 | Method for mixed fermentation of feed yeast protein particles by amino acid mother liquor and corn steep liquor |
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