CN116574771B - Alginic acid fermentation broth and preparation method and application thereof - Google Patents
Alginic acid fermentation broth and preparation method and application thereof Download PDFInfo
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- C05F11/00—Other organic fertilisers
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- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/10—Addition or removal of substances other than water or air to or from the material during the treatment
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- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
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- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0084—Guluromannuronans, e.g. alginic acid, i.e. D-mannuronic acid and D-guluronic acid units linked with alternating alpha- and beta-1,4-glycosidic bonds; Derivatives thereof, e.g. alginates
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Abstract
The application provides alginic acid fermentation broth, a preparation method and application thereof, and relates to the technical field of fermentation. The preparation method of the alginic acid fermentation broth comprises the following steps: step one, cleaning and crushing kelp, adding water, stirring to obtain kelp paste, removing salt by electrodialysis, and freeze-drying to obtain kelp powder; step two, uniformly mixing kelp powder with water, and adding a compound enzyme preparation for enzymolysis to obtain an enzymolysis liquid; and thirdly, adding pediococcus pentosaceus into the enzymolysis liquid, and performing low-temperature fermentation to obtain alginic acid fermentation liquid. According to the application, the Pediococcus pentosaceus CICC 22239 is combined with a specific compound enzyme preparation to perform joint fermentation treatment on the kelp for the first time, so that the alginic acid fermentation liquor with high alginic acid content and high humic acid content is obtained, the yield of tomatoes can be effectively improved, and the kelp fermentation liquor has a certain effect on preventing and treating tomato soft rot.
Description
Technical Field
The application relates to the technical field of fermentation, in particular to alginic acid fermentation broth, and a preparation method and application thereof.
Background
The seaweed resources in China are rich, the problems of disaster inundation, difficult treatment and high cost of the seaweed resources caused by water eutrophication also exist, and the problem of seaweed pollution can be effectively solved by recycling the seaweed resources. The seaweed fertilizer prepared from the kelp at present has great advantages in the aspects of yield increase, stress resistance and soil pollution reduction, and is a natural, green and efficient novel agricultural fertilizer. Research shows that kelp contains great amount of mineral elements and vitamins, especially algal polysaccharide with high bioactivity, highly unsaturated fatty acid and natural plant growth regulator, and can stimulate the production of non-specific active factors in plant body and regulate the balance of endogenous hormone. However, the content of alginic acid in the existing alginic acid water-soluble fertilizer is only 3% -6%, namely the content of alginic acid in the alginic acid water-soluble fertilizer still needs to be improved, and the existing alginic acid water-soluble fertilizer often has the problems of poor stability and incapability of long-term storage. And the research on the seaweed fertilizer products on improving the yield and quality of the solanaceous vegetables and fruit trees is more at present, but the report on the influence of the seaweed fertilizer products on the disease resistance of plants is less. The patent CN111387211A discloses a composite microbial agent containing alginic acid, which can effectively prevent and treat root knot nematode disease, cabbage soft rot, pepper root rot, tomato damping off and pepper epidemic disease. However, no precedent for preventing and treating tomato soft rot by using alginic acid related products exists at present.
The tomato soft rot is a disease caused by Pythium aphanidermatum and occurring in tomatoes. The diseased fruits are in water immersion yellow brown or brown spots, are softened and fermented rapidly, so that the whole fruits are rotted, dense white mould layers are formed, the diseased fruits are more shed, and the light is quickly rotten. The tomato soft rot is taken as one of main diseases of tomatoes, is called three diseases of tomato seedling stage together with tomato damping-off and tomato retting, has serious damage to tomato planting and is easy to cause huge economic loss. Because agricultural control is time-consuming and labor-consuming, most of rural areas are elderly people for vegetable planting, and in order to save time and labor, medicament control is mostly adopted, but the medicament control method is improper, and the medicament resistance is easy to generate.
Disclosure of Invention
The application aims to provide an alginic acid fermentation broth with good stability, capable of improving tomato yield and improving tomato soft rot resistance, and a preparation method and application thereof.
In one aspect, the application provides a method for preparing an alginic acid fermentation broth, the method comprising the steps of:
step one, cleaning and crushing kelp, adding water, stirring to obtain kelp paste, removing salt by electrodialysis, and freeze-drying to obtain kelp powder;
step two, uniformly mixing the kelp powder with water, and adding a compound enzyme preparation accounting for 1-10% of the kelp powder in percentage by mass for enzymolysis to obtain an enzymolysis liquid; the compound enzyme preparation consists of ficin, hemicellulase, pectase and neutral protease with the mass ratio of 1 (2-4) to 4-6 to 1-2;
thirdly, adding pediococcus pentosaceus into the enzymolysis liquidPediococcus pentosaceus) CICC 22239 with the inoculation amount of 1-5 percent is fermented to obtain alginic acid fermentation liquor; the pediococcus pentosaceus is [ ]Pediococcus pentosaceus) OD of CICC 22239 600 The value is 3-4.
Preferably, the addition amount of the compound enzyme preparation is 5% of the mass of the kelp powder.
Preferably, the complex enzyme preparation consists of ficin, hemicellulase, pectase and neutral protease in the mass ratio of 1:3:5:1.
In a preferred embodiment, the complex enzyme preparation is a liquid enzyme preparation having an enzyme activity of 3 kilo-3 kilo U/g.
Preferably, the inoculum size is 2%.
The inoculation amount refers to the percentage of the volume of the transferred seed liquid to the volume of the culture liquid after inoculation.
Preferably, the OD 600 4.
OD 600 Refers to the absorption of seed liquid at 600 nm wavelengthValues.
In a preferred embodiment, the pediococcus pentosaceus is a pediococcus pentosaceus CICC 22239 seed solution, and the preparation method of the seed solution comprises the following steps:
inoculating Pediococcus pentosaceus CICC 22239 into MRS culture medium at 30deg.C, rotation speed of 200 rpm, pH of 6.8, and culturing to OD 600 4.
Pediococcus pentosaceus (L.) HeimPediococcus pentosaceus) CICC 22239, purchased from China center for type culture Collection of microorganisms.
Further, the electrodialysis desalination condition is that the voltage is 30-35V and the flow rate is 10-20L h -1 The desalting time is 1-2 h.
Preferably, the electrodialysis desalination condition is that the voltage is 31V and the flow rate is 15L.h -1 The desalting time was 1.5. 1.5 h.
Further, the kelp powder and water are mixed according to a mass ratio of 1: mixing the components (3-5).
Preferably, the kelp powder and water are mixed according to a mass ratio of 1:4, and mixing the components in proportion.
In a preferred embodiment, the kelp powder to water feed ratio is 1 g:4 mL.
Further, the enzymolysis condition is that the enzymolysis temperature is 25-40 ℃, the enzymolysis time is 0.4-0.6 h, and the enzymolysis pH value is 5-6.5.
Preferably, the enzymolysis condition is that the enzymolysis temperature is 37 ℃, the enzymolysis time is 0.5 and h, and the enzymolysis pH value is 6.
Further, the fermentation conditions are as follows: fermenting at 25-35deg.C, pH of 6-7, rotation speed of 100-300 rpm, and fermentation time of 2-4 h.
Preferably, the fermentation conditions are: the fermentation temperature was 30℃and the fermentation speed was 200 rpm, the fermentation pH was 6.8 and the fermentation time was 3 h.
Since alginic acid is easily damaged by high temperature, the application selects complex enzyme and specific fermentation strain to carry out low temperature enzymolysis and low temperature fermentation, and can keep alginic acid component in fermentation liquor to the greatest extent.
Preferably, the third step further comprises a step of inactivating and filtering, wherein the inactivating temperature is 70-80 ℃ and the inactivating time is 1-5 min; more preferably, at 75℃for 5 min.
Under the inactivation condition, the activity of most enzymes and bacteria can be effectively inactivated, and the destruction of effective substances in the alginic acid fermentation broth can be avoided.
In a preferred embodiment, the preparation method of the alginic acid fermentation broth specifically comprises the following steps:
step one, cleaning kelp to remove impurities, crushing, adding water with the same volume, stirring uniformly to obtain kelp paste, and carrying out electrodialysis desalination on the kelp paste, wherein the voltage is 30-35V, and the flow rate is 10-20L h -1 Desalting 1-2-h, and freeze-drying the desalted kelp paste to obtain kelp powder;
step two, mixing the kelp powder with water according to a feed liquid ratio of 1 g: (3-5) mixing the materials evenly, adding a compound enzyme preparation accounting for 1-10% of the mass of the kelp powder for enzymolysis, wherein the temperature is 25-40 ℃, the pH=5-6.5 and the enzymolysis is 0.4-0.6 and h to obtain an enzymolysis solution; the compound enzyme preparation consists of ficin, hemicellulase, pectase and neutral protease with the mass ratio of 1 (2-4) to 4-6 to 1-2;
thirdly, adding pediococcus pentosaceus with the inoculum size of 1% -5% into the enzymolysis liquid, fermenting at low temperature under the specific fermentation conditions of 25-35 ℃, 100-300 rpm, pH=6-7 and 2-4 h, inactivating for 1-5 min at 70-80 ℃ after the fermentation is finished, and filtering to obtain the alginic acid fermentation liquid.
Pediococcus pentosaceus is Pediococcus pentosaceusPediococcus pentosaceus) CICC 22239, purchased from China center for type culture Collection of microorganisms.
The culture method of the seed liquid comprises the following steps: inoculating Pediococcus pentosaceus CICC 22239 into MRS culture medium at 25-40deg.C, rotation speed of 100-300 rpm, pH of 6-7, culturing about 12-36 h until OD 600 3-4.
On the other hand, the application also provides the alginic acid fermentation broth prepared by the method.
On the other hand, the application also provides the alginic acid fermentation liquor prepared by the method or the application of the alginic acid fermentation liquor in preparing fertilizer.
In another aspect, the application also provides an alginic acid fermentation broth prepared by the above method or the use of an alginic acid fermentation broth as described above for promoting tomato growth, increasing tomato yield and/or enhancing tomato disease resistance.
Further, the disease is soft rot.
As described above, the application provides a preparation method of an alginic acid fermentation broth for enhancing the resistance of tomatoes to soft rot and the alginic acid fermentation broth.
In another aspect, the application also provides a fertilizer product comprising an alginic acid fermentation broth prepared by the method described above or an alginic acid fermentation broth as described above.
The application has the following beneficial effects:
1. the application discovers a compound enzyme composition for efficiently producing alginic acid, wherein a compound enzyme preparation consists of ficin, hemicellulase, pectase and neutral protease in a mass ratio of 1:3:5:1, and the alginic acid content in fermentation broth can be effectively improved by treating the compound enzyme preparation, wherein the alginic acid content can reach 30.88% at maximum, and the humic acid content can reach 42.08% at maximum;
2. according to the application, the kelp can be treated by adopting pediococcus pentosaceus CICC 22239 combined with a specific compound enzyme preparation for co-fermentation to obtain the alginic acid fermentation liquor with high alginic acid content, and humic acid is also enriched in the alginic acid fermentation liquor obtained by fermentation, so that a new production engineering bacterium is provided for the alginic acid fermentation liquor;
3. according to the application, the electrodialysis method is adopted to effectively remove inorganic salts in kelp, so that efficient desalination is realized, the influence of the inorganic salts on subsequent enzymolysis and fermentation treatment is successfully avoided, namely, the electrodialysis desalination is matched with the enzymolysis combined fermentation method in the application to maximally improve the content of active ingredients in the alginic acid fermentation broth, and compared with the existing alginic acid fermentation broth products, the alginic acid fermentation broth prepared by the method has more excellent room temperature and high temperature stability, and the shelf life of the products can be effectively prolonged under the condition that other preservative ingredients are not added;
4. the alginic acid fermentation broth prepared by the method is used as a fertilizer to be sprayed on tomatoes, so that the yield of the tomatoes can be effectively improved, and the tomato fermentation broth can play an excellent role in preventing and treating tomato soft rot.
Detailed Description
In order to more clearly illustrate the general concept of the present application, the following detailed description is given by way of example. In the following description, numerous specific details are set forth in order to provide a more thorough understanding of the present application. It will be apparent, however, to one skilled in the art that the application may be practiced without one or more of these details. In other instances, well-known features have not been described in detail in order to avoid obscuring the application.
In the following embodiments, unless specified otherwise, the reagents or apparatus used are conventional products available commercially without reference to the manufacturer.
Wherein, MRS culture medium is purchased from Beijing Soy Bao technology Co., ltd; ficin (CAS number: 9001-33-6), hemicellulase (CAS number: 9025-56-3), pectase (CAS number: 9032-75-1), neutral protease (CAS number: 9014-01-1) were purchased from Sigma-Aldrich; pediococcus pentosaceus is Pediococcus pentosaceusPediococcus pentosaceus) CICC 22239, purchased from China center for type culture Collection; the tomatoes used in the experiment adopt tomato medium vegetable No. 5; the electrodialysis device adopts an experimental electrodialysis system BONA-ED-18, which is purchased from Shandong Bona group.
The culture method of the seed liquid comprises the following steps: inoculating Pediococcus pentosaceus in MRS medium at 30deg.C and rotation speed of 200 rpm at pH of 6.8, culturing at about 24. 24 h to OD 600 Is 4 (OD) 600 At 4, the viability of the strain can be maximized).
MRS medium: casein 10.0 g, beef extract 10.0 g, yeast powder 5.0 g, glucose 5.0 g, sodium acetate 5.0 g, diammonium citrate 2.0 g, tween-80.0 g, K 2 HPO 4 2.0 g、MgSO 4 ·7H 2 O 0.2 g、MnSO 4 .H 2 O 0.05 g、CaCO 3 20.0 g, agar 15.0 g, distilled water 1.0L.
The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer.
Example 1 screening experiment of Complex enzyme
In this embodiment, the complex enzyme species for preparing the alginic acid fermentation broth by enzymolysis of kelp are screened, and the specific screening process includes: cleaning herba Zosterae Marinae, removing impurities, and dividing into pieces (each piece is about 1 cm) 2 ) Pulverizing, and lyophilizing to obtain herba Zosterae Marinae powder; mixing herba Zosterae Marinae powder with water, and adding compound enzyme preparations of different quality and different types for enzymolysis to obtain enzymolysis solution, i.e. alginic acid fermentation liquor. Alginic acid content in the fermentation broth was determined using the NY/T3174-2017 specification and repeated three more times per group, all data were averaged and specific results are shown in Table 1.
TABLE 1
Note that: the proportion of the complex enzyme is the mass ratio of the complex enzyme.
As can be seen from the results in Table 1, the present application found a complex enzyme composition for efficiently producing alginic acid, and the alginic acid content in the fermentation broth can be effectively improved by treating the complex enzyme preparation. The application also detects the alginic acid content in a commercially available alginic acid water-soluble fertilizer, and the content is only 6.34%, while the alginic acid content in the alginic acid fermentation liquid produced by the fermentation of the specific complex enzyme in the embodiment can reach 16.01% at the highest.
In addition, in the embodiment, the parameters such as the feed liquid ratio of the kelp powder to water, the type and the combination proportion of the compound enzyme preparation, the addition amount of the compound enzyme, the enzymolysis temperature, the enzymolysis time, the enzymolysis pH and the like have certain influence on the alginic acid content in the final alginic acid fermentation broth. In this embodiment, the preferable ratio of kelp powder to water is 1 g:4 mL, wherein the compound enzyme preparation comprises ficin, hemicellulase, pectase and neutral protease in a mass ratio of 1:3:5:1, and the compound enzyme is added to 5% of the kelp powder by mass for enzymolysis at 37 ℃ for 0.5 h, and the alginic acid content in the obtained alginic acid fermentation broth is highest under the condition of enzymolysis pH of 6.
Example 2 screening experiments for fermentation broths
In order to further increase the alginic acid content and other beneficial components in the alginic acid fermentation broth, the present application attempted to use different strains for the secondary fermentation of the fermentation broth of example 1. Specifically, the enzymatic hydrolysate of example 1 was subjected to low-temperature fermentation (25℃to 35℃at a rotational speed of 100 to 300 rpm and pH=6 to 7) by adding different strains to obtain an alginic acid fermentation broth, and the alginic acid content in the fermentation broth was measured by the same method as in example 1, and the specific strains, fermentation conditions and measurement results are shown in Table 2.
TABLE 2
As can be seen from the results in Table 2, the application discovers for the first time that the kelp can be treated by co-fermentation by adopting Pediococcus pentosaceus CICC 22239 or Saccharomyces cerevisiae CICC31624 in combination with a specific complex enzyme preparation to obtain an alginic acid fermentation broth with high alginic acid content. The specific alginic acid content in the alginic acid fermentation broth is affected by the seed inoculum size and fermentation time, and can be optimized when the seed inoculum size is 2% and the fermentation time is 3 h.
In addition, in the specific operation process, the application also finds that the alginic acid fermentation broth obtained after fermentation by using Pediococcus pentosaceus CICC 22239 has obvious color change, and is supposed to have increased humic acid content, so that the humic acid content in the alginic acid fermentation broth is further measured by using DB21T 1322-2004 standard, each group is repeated three times or more, all the data are averaged, and the measurement results are shown in Table 3.
TABLE 3 Table 3
As can be seen from the results in Table 3, the humic acid content in the alginic acid fermentation broth can be increased by co-fermentation treatment of kelp using Pediococcus pentosaceus CICC 22239 in combination with a specific complex enzyme preparation. The humic acid content can reach 36.20 percent, which is larger than the humic acid content (21.18 percent) in the conventional alginic acid water-soluble fertilizer in the market.
EXAMPLE 3 kelp desalination experiments
In the actual operation process, the large pH difference between different groups of samples before enzymolysis is found, which is caused by the fact that inorganic salts in kelp cannot be completely removed in the cleaning and impurity removing process. In this example, the step of sea tangle desalination is added before the enzymolysis, considering that part of the inorganic salt may affect the subsequent enzymolysis and fermentation treatment.
The specific desalting step comprises the following steps: the kelp blocks after the impurities are removed by cleaning are crushed, the kelp blocks are mixed with water in equal volume to form kelp mud, the kelp mud is poured into a liquid medicine tank in electrodialysis equipment, tap water is injected into a liquid electrode tank, deionized water is injected into a concentrated salt liquid tank, influences of different voltages and flow rates on the desalting effect are explored, the desalting is stopped until the conductivity is stable (the treatment time is recorded), the desalting rate is measured, each group is repeated for more than three times, and all data are averaged. The salt rejection was calculated by the following formula, and the specific results are shown in Table 4.
Desalination rate = (pre-treatment conductivity-post-treatment conductivity)/(pre-treatment conductivity)
TABLE 4 Table 4
As can be seen from the results in Table 4, the electrodialysis method can effectively remove inorganic salts in kelp, realize efficient desalination, and successfully avoid the influence of inorganic salts on subsequent enzymolysis and fermentation treatment.
Example 4
Test example 1
The test example provides a preparation method of alginic acid fermentation broth, which comprises the following steps:
step one, cleaning kelp to remove impurities, crushing, adding water with the same volume, stirring uniformly to obtain kelp paste, and carrying out electrodialysis desalination on the kelp paste, wherein the voltage is 31V, and the flow rate is 15L hours -1 Desalting 1.5. 1.5 h, and freeze-drying the desalted kelp paste to obtain kelp powder;
step two, mixing the kelp powder with water according to a feed liquid ratio of 1 g:4 mL, adding a compound enzyme preparation accounting for 5% of the mass of the kelp powder for enzymolysis, wherein the temperature is 37 ℃, the enzymolysis is carried out for 0.5 h, and the pH=6, so as to obtain an enzymolysis liquid; the compound enzyme preparation consists of ficin, hemicellulase, pectase and neutral protease in the mass ratio of 1:3:5:1;
and thirdly, adding pediococcus pentosaceus into the enzymolysis liquid, wherein the inoculation amount is 2%, fermenting at low temperature, wherein the specific fermentation conditions are 30 ℃, the rotating speed is 200 rpm, the pH=6.8, the fermentation is 3 h, inactivating the fermentation liquid at 75 ℃ for 5 min after the fermentation is finished, and filtering to obtain the alginic acid fermentation liquid.
Pediococcus pentosaceus is Pediococcus pentosaceusPediococcus pentosaceus) CICC 22239 purchased from China center for type culture Collection of Industrial microorganisms
The culture method of the seed liquid comprises the following steps: inoculating Pediococcus pentosaceus in MRS medium at 30deg.C and rotation speed of 200 rpm at pH of 6.8, culturing at about 24. 24 h to OD 600 4.
The alginic acid content obtained in this test example was 30.88% and the humic acid content was 42.08% by the same method as in example 1.
Test example 2
The test example differs from test example 1 only in that the complex enzyme preparation was directly added to the kelp puree without performing the freeze-drying treatment.
Test example 3
The test example differs from test example 1 only in that the low temperature fermentation conditions were 40℃and the rotation speed was 200 rpm, and in that the pH=6.8.
Test example 4
The test example differs from test example 1 only in that the low temperature fermentation conditions were 30℃and the rotation speed was 200 rpm, and the pH=8.
Test example 5
The test example differs from test example 1 only in that the inactivation temperature is 85 ℃.
Test example 6
The test example differs from test example 1 only in that the inactivation time is 10 min.
Test example 7
The test example differs from test example 1 only in that no inactivation was performed.
Test example 8
The test example differs from test example 1 only in that no desalting was performed.
Comparative example
Commercially available alginic acid water-soluble fertilizer products.
Stability test
In this example, stability tests were conducted using the alginic acid fermentation broths of test examples 1 to 8 and comparative example, and specifically, the alginic acid fermentation broths were respectively placed in a room temperature environment (25 ℃) and a high temperature environment (50 ℃) for 6 months, the states of the alginic acid fermentation broths were observed, and the observation results are shown in table 5.
TABLE 5
As shown in table 5, test example 1, test example 3 and test example 4 can maintain long-term system stability both in a room temperature environment and in a high temperature environment.
The comparison between test example 8 and test example 1 shows that the difference is that the test example 8 does not perform the desalting operation, but the alginic acid fermentation broth obtained in the test example 8 is subjected to flocculent precipitation after being placed at room temperature for 6 months, so that the effect of enzymolysis and fermentation can be affected by too much inorganic salt in kelp, the stability of the final product can be affected, the product can not be stored for a long time, and the problem of too short shelf life exists.
In addition, as can be seen from comparison of test example 2 and test example 1, on the one hand, in the technical scheme of the application, the electrodialysis desalination is matched with the freeze drying treatment to remove inorganic salt and impurity components in kelp to the greatest extent, because the electrodialysis desalination can only remove charged ions in water, and the removal effect of the electrodialysis is not good for uncharged ions or impurities such as silicon, boron and the like in water, other impurities are removed by adopting the freeze drying treatment, the subsequent enzymolysis and fermentation treatment are facilitated, the stability of the product in a high-temperature environment is improved, on the other hand, the freeze drying to remove the moisture in kelp mud is facilitated, the accurate control of the feed-liquid ratio and other enzymolysis conditions in the subsequent enzymolysis step is also facilitated, the content of effective substances in the product is improved, and the product quality is ensured.
In addition, it can be seen from the comparison of test examples 5 to 7 with test example 1 that the inactivation temperature, the inactivation time and whether the inactivation is performed affect the stability of the final alginic acid fermentation broth because alginic acid is easily decomposed under high temperature environment to form unstable components, whereas in order to avoid this, the complex enzyme preparation used in the present application is a low temperature enzymolysis preparation, the zymophyte is also a low temperature fermentation strain, and both of them can be inactivated at a lower temperature. The method can avoid damage to the alginic acid product to the greatest extent in the use process so as to obtain a fermentation product with high alginic acid content, and can inactivate enzymes and bacteria at a lower temperature after fermentation is completed so as to prevent the enzymes and bacteria from continuing fermentation or enzymolysis so as to influence the product performance (like test example 7). Thus, test example 5 demonstrates that when the inactivation temperature reaches 85 ℃, alginic acid components in the product are destroyed, resulting in unstable system. Test example 6 demonstrates that too long an inactivation time can also have an effect on the stability of the system. As shown in test example 1, the inactivation at 75 ℃ for 5 min can realize the low-temperature rapid inactivation of specific complex enzymes and fermentation strains, and avoid adverse effects on the product components and the product performance.
Field test
In this example, field trials were conducted using the alginic acid fermentation broths of test examples 1 to 8 and comparative examples. The growth condition of tomato seedlings is detected, specifically, the alginic acid fermentation liquor and the comparative example in test examples 1-8 are respectively sprayed on tomato seedlings in different groups in an equivalent amount, water is sprayed as a blank control, at least 50 tomato seedlings in each group are selected for experiment, healthy tomato seedlings with consistent growth period are selected for experiment, and finally, the single fruit quality of the tomatoes is detected in the same day in the mature period (50 days after pollination), and all the data are averaged. Meanwhile, tomato seedlings are planted in soil with high soft rot (containing soft rot strains) and soil with high damping-off respectively by adopting the same method for grouping, the tomato seedlings are treated until the mature period (50 days after pollination), the morbidity of the tomato plants is recorded, and the calculating method of the morbidity is as follows. The detection results of tomato growth and disease resistance are shown in Table 6.
Incidence (%) = number of plants to be affected/total number of plants×100%
TABLE 6
As can be seen from the data in Table 6, the alginic acid fermentation broth prepared by the method of test example 1 is applied to tomatoes as fertilizer, which is helpful for improving tomato yield, and has excellent effect especially on preventing and treating tomato soft rot.
The foregoing is merely exemplary of the present application and is not intended to limit the present application. Various modifications and variations of the present application will be apparent to those skilled in the art. Any modification, equivalent replacement, improvement, etc. which come within the spirit and principles of the application are to be included in the scope of the claims of the present application.
Claims (4)
1. A preparation method of alginic acid fermentation broth for improving cotton rot resistance of tomatoes is characterized by comprising the following steps:
step one, cleaning kelp to remove impurities, crushing, adding water with the same volume, stirring uniformly to obtain kelp paste, and carrying out electrodialysis desalination on the kelp paste, wherein the voltage is 31V, and the flow rate is 15L hours -1 Desalting 1.5. 1.5 h, and desaltingFreeze-drying the kelp paste to obtain kelp powder;
step two, mixing the kelp powder with water according to a feed liquid ratio of 1 g:4 mL, adding a compound enzyme preparation accounting for 5% of the mass of the kelp powder for enzymolysis, wherein the temperature is 37 ℃, the enzymolysis is carried out for 0.5 h, and the pH=6, so as to obtain an enzymolysis liquid; the compound enzyme preparation consists of ficin, hemicellulase, pectase and neutral protease in the mass ratio of 1:3:5:1;
adding pediococcus pentosaceus into the enzymolysis liquid, wherein the inoculation amount is 2%, fermenting at low temperature, wherein the specific fermentation conditions are 30 ℃, the rotating speed is 200 rpm, the pH=6.8, the fermentation is 3 h, inactivating the fermentation liquid at 75 ℃ for 5 min after the fermentation is finished, and filtering to obtain alginic acid fermentation liquid; the pediococcus pentosaceus is @ Pediococcus pentosaceusPediococcus pentosaceus）CICC 22239，OD 600 The value is 4.
2. An alginic acid fermentation broth prepared by the method of claim 1.
3. Use of an alginic acid fermentation broth prepared by the method of claim 1 or an alginic acid fermentation broth according to claim 2 for preparing fertilizers.
4. A fertilizer product comprising the alginic acid fermentation broth prepared by the method of claim 1 or the alginic acid fermentation broth of claim 2.
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| CN109734527A (en) * | 2019-03-25 | 2019-05-10 | 广西中连智浩科技有限公司 | A kind of preparation method of novel alga Controlled Release Fertilizer |
| CN111348977A (en) * | 2020-04-23 | 2020-06-30 | 青岛海力源生物科技有限公司 | Bacterial enzyme linked alginic acid fertilizer synergist and preparation method thereof |
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| CN109734527A (en) * | 2019-03-25 | 2019-05-10 | 广西中连智浩科技有限公司 | A kind of preparation method of novel alga Controlled Release Fertilizer |
| CN111348977A (en) * | 2020-04-23 | 2020-06-30 | 青岛海力源生物科技有限公司 | Bacterial enzyme linked alginic acid fertilizer synergist and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 海带酶解液的戊糖片球菌发酵及其产物的抗氧化性、抑菌性;张丽姣等;食品科学;第35卷(第21期);164-169 * |
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