CN112679272B - Biological bacterial fertilizer for saffron crocus and preparation method thereof - Google Patents
Biological bacterial fertilizer for saffron crocus and preparation method thereof Download PDFInfo
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Abstract
The invention relates to the field of traditional Chinese medicine cultivation, and particularly discloses a biological bacterial fertilizer for saffron crocus and a preparation method thereof, wherein the biological bacterial fertilizer comprises the following components in parts by weight: 35-45% of microbial bacteria, 15-25% of bentonite, 25-30% of soybean meal and 10-20% of auxiliary agent, wherein the microbial bacteria are saffron endophytic fungi and sophora tonkinensis endophytic fungi. The invention utilizes two microorganisms of saffron endophytic fungi and sophora tonkinensis endophytic fungi, and prevents and treats the root rot of the corm of saffron while improving the yield and the content of effective components of saffron; meanwhile, the application of the biological bacterial manure can avoid complicated planting operation process, reduce labor and time cost, improve the yield and the content of effective components of the saffron crocus and increase the economic benefit of the saffron crocus.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicine cultivation, and particularly relates to a saffron biological bacterial fertilizer and a preparation method thereof.
Background
Saffron (with the scientific name: Crocus sativus L.), also called Crocus sativus and saffron, is a perennial flower of Crocus in iridaceae, is a common spice and is a rare traditional Chinese medicinal material in China. The saffron is used as medicine in the upper part of pistil, and has the functions of promoting blood circulation to disperse blood clots, cooling blood, detoxicating, dispelling melancholy and tranquilizing. The saffron is originally produced in the Mediterranean region, the introduction of the crocus was successfully introduced in the Chongming island in the Shanghai in the last 80 th century, and the Jiande city in Zhejiang is the main production area of the saffron in China at present. In the origin place, the growth condition of saffron is good, most of saffron is wild, and after the saffron is introduced into China, due to the change of conditions such as climate, soil environment and the like, particularly in the Yangtze triangle area of China, the air is humid, the rainfall is abundant, the underground corms of the saffron are easy to rot, and the yield and the quality of the saffron are influenced, so a two-stage type bud picking cultivation method is adopted, but the annual average reproduction rate of the saffron seedballs in China is only about 1.2, the annual average yield of the saffron seedballs can only be increased by about 20 percent, and a large amount of imports are still needed. Therefore, how to improve the reproductive capacity and yield of saffron crocus seed balls is an urgent problem to be solved in the aspect of cultivation technology, and is an important way to improve the quality of medicinal materials.
The endophytic fungi refer to bacteria living in healthy plant tissues and organs, are microorganisms derived from host plants in a long-term co-evolution process, and are fungi which live in stems and leaves of the host plants and complete life cycles. Research shows that the plant endophytic fungi can be produced from metabolites of similar or same active ingredients of plants, the plants infected by the endophytic fungi have the potential advantages of increased vegetative growth, enhanced drought resistance and toxin production by the endophytic fungi, the endophytic fungi are easy to ferment, culture and industrially produce, the industrial production of bioactive substances is realized by utilizing the fermentation of the endophytic fungi, the product yield can be improved, the production cost is reduced, and the market demand is met.
In the prior art, CN201810823419.5 discloses an application method of a biological bacterial fertilizer for increasing saffron yield in cultivation, in the saffron cultivation process, a biological bacterial fertilizer containing saffron endophytic fungi is applied before the saffron seed bulbs balling up, wherein the biological bacterial fertilizer is prepared by activation, cultivation and fungus cake fermentation cultivation of endophytic fungi (hansis endophytic fungi), and in the using process, tap water needs to be proportioned for spraying, so as to increase the yield of saffron and the content of active ingredients. However, during spraying, a spray with a velocity of approximately 1: 33, the ratio of tap water is matched for spraying, and considering the current climate moistening of the saffron main production area in China, the method is not particularly suitable for the saffron with root rot which can occur in each growth stage, and the root rot are easy to cause; in addition, CN201610757180.7 discloses a method for breeding saffron seedballs, wherein the method applies a chemical and a biological comprehensive antiseptic method to the saffron seedballs, and interplanting the saffron seedballs with grapes in a greenhouse, and combines the application of the existing cultivation technology, so as to improve the moderate temperature of the saffron in the winter breeding period and reduce rain in the summer dormancy period, so that the incidence rate of the saffron seedball rot is greatly reduced, thereby changing the original 'two-stage' cultivation technology and increasing the yield of the saffron. Therefore, the following problems still exist in the prior art: can not only improve the yield and the effective components of saffron crocus, but also prevent root rot and simultaneously achieve the aim of reducing the cost.
Disclosure of Invention
The invention aims to overcome the defects of the prior art, and provides a biological bacterial fertilizer for saffron crocus and a preparation method thereof, so as to solve the problems that the yield and effective components cannot be increased, root rot is prevented, and the cost cannot be reduced simultaneously in the prior art.
In order to solve the technical problems, the invention adopts a technical scheme that: the biological bacterial fertilizer for saffron crocus is provided and consists of the following components in parts by weight: 35-45% of microbial bacteria, 15-25% of bentonite, 25-30% of soybean meal and 10-20% of auxiliary agent, wherein the microbial bacteria are saffron endophytic fungi and sophora tonkinensis endophytic fungi.
Preferably, the biological bacterial fertilizer consists of the following components in parts by weight: 40% of microbial bacteria, 20% of bentonite, 25% of soybean meal and 15% of an auxiliary agent.
The biological bacterial fertilizer is a microbial fertilizer, is a product which leads crops to obtain a specific fertilizer effect due to the life activity of microorganisms, and is a fertilizer used in agricultural production. The biological fertilizer in the broad sense is a product which enables crops to obtain specific fertilizer effects through the life activities of microorganisms, is also called as an inoculant or a bacterial fertilizer, contains nutrient elements required by the crops, contains products of the microorganisms, is a combination of organisms, organic matters and inorganic matters, and can provide various nutrient elements required by the growth and development of the crops. Researches show that the endophytic fungi can produce metabolites with active components similar to or the same as those of host plants, and most of the metabolites are in a reciprocal symbiotic relationship with the host plants, the endophytic fungi live in the plants and grow and develop by utilizing nutrient substances of the plants, and the endophytic fungi can produce substances for stimulating the growth of the plants to promote the growth of the plants, so that the product yield is improved, the cost is reduced, and the market demands are met. In the above technical scheme, the saffron endophytic fungi and the sophora tonkinensis endophytic fungi are used as microbial sources of the biological bacterial manure, wherein the biological bacterial manure prepared from the saffron endophytic fungi is used, researches show that the yield of saffron and the content of effective components can be greatly improved, the number of necrotic bulbs is reduced, the yield of medicinal materials is improved by 60%, the yield of single-plant corm medicinal materials is increased by 41.8%, the weight of 30 saffron stigma is improved by 18%, it can be seen that the yield can be obviously improved by using the saffron endophytic fungi, and the crude saffron endophytic fungi polysaccharide has the effects of removing DPPH free radicals, hydroxyl free radicals and superoxide anion free radicals, and has obvious antioxidant activity. The sophora tonkinensis endophytic fungus has a strong inhibiting effect on root rot germs as an endophytic fungus for preventing and treating the root rot, has outstanding potential in ecological prevention and treatment on the root rot, and has a good preventing and treating effect on the problem that the corm of saffron crocus is easy to rot. In addition, bentonite is used as a filler to dilute the fertilizer raw materials, so that the using amount of the fertilizer raw materials is reduced, the dispersibility of the fertilizer is improved, and the mechanical crushing is facilitated; the soybean flour is used as an adhesive, thereby increasing the adhesion to other components of the bio-fertilizer, and the soybean flour is rich in protein and unsaturated fatty acid, can provide a nitrogen source for the fertilizer, and simultaneously improves the land quality. By using the biological bacterial fertilizer in the technical scheme, the yield of saffron crocus and the content of effective components can be effectively improved, and the root rot can be prevented and treated.
Preferably, the auxiliary agent comprises fatty alcohol-polyoxyethylene ether, sodium dodecyl benzene sulfonate, sodium methylene dinaphthalene sulfonate and dichloropropene.
In the technical scheme, the fatty alcohol-polyoxyethylene ether, the sodium dodecyl benzene sulfonate, the sodium methylene dinaphthalene sulfonate and the dichloropropene are used as the auxiliary agents, the fatty alcohol-polyoxyethylene ether, the sodium dodecyl benzene sulfonate and the sodium methylene dinaphthalene sulfonate are used as the surfactants, wherein the fatty alcohol-polyoxyethylene ether is also called peregal in industrial production and has the functions of moistening and moisture permeability, the sodium dodecyl benzene sulfonate simultaneously has hydrophilic groups and hydrophilic groups in molecules and can reduce the surface tension so as to play a role in emulsification, the sodium methylene dinaphthalene sulfonate plays a role in dispersing agent, the dichloropropene is used as a pesticide and comprises the mixed dichloropropene and cis-dichloropropene, the fatty alcohol-polyoxyethylene ether, the sodium dodecyl benzene sulfonate, the sodium methylene dinaphthalene sulfonate and the dichloropropene are used as the auxiliary agents, and the effective utilization efficiency of the biological bacterial fertilizer can be improved, time cost and labor cost are reduced.
Preferably, the number ratio of the concealed safflower endophytic fungi to the sophora tonkinensis endophytic fungi in unit volume is 3-7.
Further preferably, the number ratio of the concealed safflower endophytic fungi to the sophora tonkinensis endophytic fungi in unit volume is 5.
In the technical scheme, the quantity ratio of the concealed safflower endophytic fungi to the Vietnamese sophora endophytic fungi in unit volume is set to be 3-7, so that the quantity of the saffron endophytic fungi is greater than that of the Vietnamese sophora endophytic fungi, the effect of improving the yield and the content of effective components of the saffron endophytic fungi is fully exerted, otherwise, the quantity of the Vietnamese sophora endophytic fungi is greater than that of the saffron endophytic fungi, the generation effect of the saffron endophytic fungi is not obvious due to the fact that the Vietnamese sophora endophytic fungi robusts survival and growth resources, and the yield and the effective components of the saffron cannot be effectively improved. The quantity ratio of the hidden safflower endophytic fungi to the sophora tonkinensis endophytic fungi in unit volume is 3-7, and particularly when the quantity ratio is 5, the saffron endophytic fungi and the sophora tonkinensis endophytic fungi can respectively play a role, the corm of the obtained saffron is basically not rotten in the growth process, the yield of the obtained saffron filaments is obviously increased, and the effective content is obviously increased.
Preferably, the collection number of the saffron endophytic fungi is CCTCC No. M2013472.
Preferably, the preservation number of the sophora tonkinensis endophytic fungi is CGMCC No. 10108.
The saffron endophytic fungi is selected from saffron endophytic fungi (Penicillium sp.), the preservation number of the saffron endophytic fungi (Penicillium sp.) is CCTCC No. M2013472, and crude saffron endophytic fungi polysaccharide has remarkable antioxidant activity; at 1.00mg/mL, the clearance rate of DPPH free radicals is 83.45 +/-0.18%; at 1.600mg/mL, the clearance rate of hydroxyl radical is 81.31 +/-1.33%; at 6.0000mg/mL, the scavenging rate of superoxide anion free radicals is 53.28 +/-1.72%, the saffron endophytic fungi has obvious antioxidation effect, the number of bad bulbs can be obviously reduced, the yield of medicinal materials is improved by 64%, the yield of medicinal materials of a single plant corm is improved by 43.6%, the weight of 30 saffron stigma is improved by 20%, and therefore, the saffron endophytic fungi is selected as one of the microbial compositions of the biological bacterial manure. The sophora tonkinensis endophytic fungus is selected from sophora tonkinensis endophytic fungus (Rheoxocercosporidium sp.), and the research shows that the deposit number of the sophora tonkinensis endophytic fungus (Rheoxocercosporidium sp.): CGMCC No.10108 has better prevention and treatment effects on root rot, for example, CN201510979067.9 provides that sophora tonkinensis endophytic fungus TRXY-46 has obvious prevention and treatment effects on panax notoginseng root rot, the inhibition rate on panax notoginseng root rot can reach 58%, and in the planting process of saffron, the sophora tonkinensis endophytic fungus can be used for effectively preventing the root rot, and the necrotic tissue and the root rot rate are obviously reduced to 2.3%. Therefore, the two microorganisms can solve the problems of increasing yield and effective components, preventing root rot and reducing cost in the prior art
The invention also provides a preparation method of the biological bacterial fertilizer for saffron crocus, which is characterized by comprising the following steps of:
s1 culture of microorganism: respectively placing saffron endophytic fungi and sophora tonkinensis endophytic fungi on respective nutrient agar culture medium, culturing at 28 deg.C for 7-10 days;
s2 fermented mushroom cake: respectively preparing the saffron endophytic fungi and the sophora tonkinensis endophytic fungi obtained in the step S1 into fungus cakes, inoculating the fungus cakes to respective culture media for fermentation and culture, wherein the fermentation temperature of the saffron endophytic fungi is 25-28 ℃, the rotating speed of a shaking table is 100-200rpm, and the fermentation period is 5-10 days; the fermentation temperature of the sophora tonkinensis endophytic fungi is 25-28 ℃, the rotation speed of a shaking table is 100-200rpm, and the fermentation period is 50-60 days;
s3, preparing the biological bacterial fertilizer: mixing the solid saffron endophytic fungi obtained in the step S2 and the sophora tonkinensis endophytic fungi according to the following components by weight: 35-45% of microbial bacteria, 15-25% of bentonite, 25-30% of soybean meal and 10-20% of an auxiliary agent; and grinding the obtained mixture to obtain the biological bacterial fertilizer.
Preferably, the culture medium of saffron endophytic fungi in S2 comprises the following components by weight: 2g of yeast extract, 3g of peptone, 7g of glucose and 2g of sodium chloride, and water was added to obtain 1000ml of PYG liquid medium.
Preferably, the culture medium of the sophora tonkinensis endophytic fungi in the S2 is a sterile solid culture medium and consists of the following components in parts by weight: 400g potato, 20g dextrose, and 20g sucrose.
In the technical scheme, the saffron endophytic fungi and the sophora tonkinensis endophytic fungi are respectively cultured and fermented, the growth condition change of the two fungi caused by the co-culture and fermentation of two microorganisms is avoided, so that the crude polysaccharide and other various chemical substances are qualitatively changed, the aims and effects of increasing the yield and the effective components and preventing the root rot are not achieved, the culture and fermentation conditions of the two endophytic fungi are also different, the saffron endophytic fungi uses a PYG liquid culture medium, the fermentation temperature is 25-28 ℃, the fermentation period is shorter by 5-10 days, the sophora tonkinensis endophytic fungi uses a solid culture medium, the fermentation period is longer by 50-60 days, and the saffron endophytic fungi and the sophora tonkinensis endophytic fungi are separately cultured and fermented, so that the saffron endophytic fungi and the sophora tonkinensis endophytic fungi with higher quality are obtained.
The invention has the advantages that:
1. the two microorganisms of saffron endophytic fungi and sophora tonkinensis endophytic fungi are utilized to prevent and control the root rot of the corm of saffron while improving the yield and the content of effective components of saffron;
2. The biological bacterial fertilizer is applied, the complex planting operation process is avoided, the labor and time cost can be effectively reduced, the yield and the content of effective components of saffron crocus are improved, and the economic benefit is increased.
Detailed Description
The invention discloses a biological bacterial fertilizer for saffron crocus and a preparation method thereof, and the invention is described in more detail below.
The invention is characterized in that the invention utilizes two microorganisms of saffron endophytic fungi and sophora tonkinensis endophytic fungi to prevent and control the root rot of the corm of saffron while improving the yield and the content of effective components of saffron.
The following is a description of the preferred embodiments of the present invention and a further description of the technical solutions of the present invention, but the following embodiments are only preferred embodiments of the present invention and do not limit the present invention.
Example 1:
a biological bacterial fertilizer for saffron crocus, which consists of the following components in parts by weight: 40% of microbial bacteria, 20% of bentonite, 25% of soybean flour and 15% of auxiliary agents, wherein the microbial bacteria are saffron endophytic fungi and sophora tonkinensis endophytic fungi, the number ratio of the concealed saffron endophytic fungi to the sophora tonkinensis endophytic fungi in unit volume is 5, the saffron endophytic fungi (Pelionium sp.) with the preservation number of CCTCC No. M2013472 is used, the sophora tonkinensis endophytic fungi (Rheoxocrosporium sp.) with the preservation number of CGMCC No.10108 is used as the sophora tonkinensis endophytic fungi, and the auxiliary agents are fatty alcohol polyoxyethylene ether, sodium dodecyl benzene sulfonate, sodium methylene dinaphthalene sulfonate and dichloropropene.
Example 2:
a biological bacterial fertilizer for saffron crocus, which consists of the following components in parts by weight: 35% of microbial bacteria, 15% of bentonite, 30% of soybean flour and 20% of auxiliary agents, wherein the microbial bacteria are saffron endophytic fungi and sophora tonkinensis endophytic fungi, the number ratio of the concealed saffron endophytic fungi to the sophora tonkinensis endophytic fungi in unit volume is 3, the saffron endophytic fungi (pellicium sp.) with the preservation number of CCTCC No. M2013472 are used, the sophora tonkinensis endophytic fungi (Rheoxocrosporium sp.) with the preservation number of CGMCC No.10108 are used as the sophora tonkinensis endophytic fungi, and the auxiliary agents are fatty alcohol polyoxyethylene ether, sodium dodecyl benzene sulfonate, sodium methylene dinaphthalene sulfonate and dichloropropene.
Example 3:
a biological bacterial fertilizer for saffron crocus, which consists of the following components in parts by weight: 45% of microbial bacteria, 25% of bentonite, 25% of soybean flour and 10% of auxiliary agents, wherein the microbial bacteria are saffron endophytic fungi and sophora tonkinensis endophytic fungi, the number ratio of the concealed saffron endophytic fungi to the sophora tonkinensis endophytic fungi in unit volume is 7, the saffron endophytic fungi (Pelionium sp.) with the preservation number of CCTCC No. M2013472 is used, the sophora tonkinensis endophytic fungi (Rheoxocrosporium sp.) with the preservation number of CGMCC No.10108 is used as the sophora tonkinensis endophytic fungi, and the auxiliary agents are fatty alcohol polyoxyethylene ether, sodium dodecyl benzene sulfonate, sodium methylene dinaphthalene sulfonate and dichloropropene.
Example 4:
a preparation method of a biological bacterial fertilizer for saffron crocus is used for preparing the biological bacterial fertilizer of the embodiment 1, and comprises the following specific steps:
s1 culture of microorganism: culturing stigma croci Sativi endophytic fungi in culture medium (1000 ml PYG liquid culture medium prepared from 2g yeast extract, 3g peptone, 7g glucose, and 2g sodium chloride, and water at 28 deg.C for 7-10 days); the sophora tonkinensis endophytic fungi is placed on a culture medium for culturing, the culture medium is a sterile solid culture medium prepared from 400g of potatoes, 20g of dextrose and 20g of sucrose, the culture temperature is 28 ℃, and the culture time is 7-10 days.
S2 fermented mushroom cake: preparing the saffron endophytic fungi obtained in the step S1 into fungus cakes, inoculating the fungus cakes to a culture medium for sterilization and preservation, and performing fermentation culture at the fermentation temperature of 25-28 ℃, the rotating speed of a shaking table of 100-200rpm and the fermentation period of 5-10 days; preparing the sophora tonkinensis endophytic fungi obtained in the step S1 into a fungus cake, inoculating the fungus cake to a sterilized and preserved culture medium, and performing fermentation culture at the fermentation temperature of 25-28 ℃, the rotation speed of a shaking table of 100-200rpm and the fermentation period of 50-60 days.
S3, preparing the biological bacterial fertilizer: mixing the solid saffron endophytic fungi and the solid sophora tonkinensis endophytic fungi obtained in the step S2 according to the weight components in the example 1, and grinding the obtained mixture to obtain the biological bacterial fertilizer.
Example 5:
a method for preparing a biological bacterial fertilizer for saffron, which is different from the method in example 4, and is used for preparing the biological bacterial fertilizer in example 2, wherein the components are mixed according to the weight in example 2 in step S3.
Example 6:
a method for preparing a biological bacterial fertilizer for saffron, which is different from the embodiment 4, the method is used for preparing the biological bacterial fertilizer of the embodiment 3, wherein the components are mixed according to the weight in the embodiment 3 in the step S3.
Comparative example 1:
a biological bacterial fertilizer for saffron crocus, which consists of the following components in parts by weight: 40% of microbial bacteria, 20% of bentonite, 25% of soybean flour and 15% of an auxiliary agent, wherein the microbial bacteria are saffron endophytic fungi, the saffron endophytic fungi (Peniciliumpsp) with the preservation number of CCTCC No. M2013472 are used, and the auxiliary agent is fatty alcohol-polyoxyethylene ether, sodium dodecyl benzene sulfonate, sodium methylene dinaphthalene sulfonate and dichloropropene.
Comparative example 2:
a biological bacterial fertilizer for saffron crocus, which consists of the following components in parts by weight: 40% of microbial bacteria, 20% of bentonite, 25% of soybean meal and 15% of an auxiliary agent, wherein the microbial bacteria are sophora tonkinensis endophytic fungi, the sophora tonkinensis endophytic fungi (Rhexocerosporium sp.) with the preservation number of CGMCC No.10108 are used, and the auxiliary agent is fatty alcohol polyoxyethylene ether, sodium dodecyl benzene sulfonate, sodium methylene dinaphthalene sulfonate and dichloropropene.
Comparative example 3:
a fertilizer is prepared from bentonite 33.3%, soybean powder 41.7% and assistant 25%, wherein the assistant is fatty alcohol-polyoxyethylene ether, sodium dodecylbenzenesulfonate, sodium methylenedinaphthalene sulfonate and dichloropropene.
Test examples 1-3 and comparative examples 1-3:
the biological bacterial fertilizers obtained in the examples 1 to 3 and the comparative examples 1 to 3 are respectively and sequentially marked as biological bacterial fertilizer 1, biological bacterial fertilizer 2, biological bacterial fertilizer 3, biological bacterial fertilizer 4, biological bacterial fertilizer 5 and biological bacterial fertilizer 6.
Selecting 18.0 + -2.0 g stigma croci Sativi corm, and performing two-stage cultivation, i.e. indoor flower collection and field seed ball breeding, wherein in the field seed ball breeding process, the row spacing is 10cm × 20cm, and the depth is 8-10cm, covering soil of 5-8cm, weeding in time, draining and loosening soil after rain, and fertilizing biological bacterial fertilizer 1-6 with proper amount of tap water before pilling, wherein the fertilizing amount is 6ml/m2. The yield, effective component content and root damage rate of saffron are shown in table 1:
the embodiments described herein are merely exemplary and not restrictive, and all changes that come within the meaning and range of equivalents of the claims and the equivalents thereof or the equivalents thereof may be embraced therein.
Claims (7)
1. The biological bacterial fertilizer for saffron crocus is characterized by comprising the following components in parts by weight: 35% -45% of microbial bacteria, 15% -25% of bentonite, 25% -30% of soybean flour and 10% -20% of an auxiliary agent, wherein the microbial bacteria are saffron endophytic fungi with the preservation number of CCTCC No. M2013472 and Vietnamese sophora endophytic fungi with the preservation number of CGMCC No.10108, and the quantity ratio of the saffron endophytic fungi to the Vietnamese sophora endophytic fungi in unit volume is 3-7.
2. The biological bacterial fertilizer for saffron crocus according to claim 1, characterized in that it consists of the following components by weight: 40% of microbial bacteria, 20% of bentonite, 25% of soybean meal and 15% of an auxiliary agent.
3. The biological bacterial fertilizer for saffron crocus according to claim 1, wherein said adjuvant comprises fatty alcohol-polyoxyethylene ether, sodium dodecylbenzene sulfonate, sodium methylene dinaphthalene sulfonate and dichloropropene.
4. The biological bacterial fertilizer for saffron crocus according to claim 1, wherein the ratio of the number of said saffron endophytic fungi and said sophora tonkinensis endophytic fungi per unit volume is 5.
5. A process for the preparation of biological bacterial manure for saffron crocus according to any of the claims from 1 to 4, characterized in that it comprises the following steps:
s1 culture of microorganisms: respectively placing saffron endophytic fungi and sophora tonkinensis endophytic fungi on respective nutrient agar culture medium, culturing at 28 deg.C for 7-10 days;
s2 fermented mushroom cake: respectively preparing the saffron endophytic fungi and the sophora tonkinensis endophytic fungi obtained in the step S1 into fungus cakes, inoculating the fungus cakes to respective culture mediums for fermentation and culture, wherein the fermentation temperature of the saffron endophytic fungi is 25-28 ℃, the rotating speed of a shaking table is 100-200rpm, and the fermentation period is 5-10 days; the fermentation temperature of the sophora tonkinensis endophytic fungi is 25-28 ℃, the rotation speed of a shaking table is 100-200rpm, and the fermentation period is 50-60 days;
s3, preparing the biological bacterial fertilizer: mixing the solid saffron endophytic fungus obtained from S2 and the solid Vietnamese sophora endophytic fungus according to the following components by weight: 35-45% of microbial bacteria, 15-25% of bentonite, 25-30% of soybean meal and 10-20% of auxiliary agent; and grinding the obtained mixture to obtain the biological bacterial fertilizer.
6. The preparation method of biological bacterial manure for saffron crocus according to claim 5, wherein the culture medium of saffron endophytic fungi in S2 comprises the following components by weight: 2g of yeast extract, 3g of peptone, 7g of glucose and 2g of sodium chloride, and water was added to obtain 1000ml of PYG liquid medium.
7. The preparation method of biological bacterial manure for saffron crocus according to claim 5, wherein the culture medium of the sophora tonkinensis endophytic fungi in S2 is a sterile solid culture medium, which consists of the following components by weight: 400g potato, 20g dextrose, and 20g sucrose.
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| CN104726342B (en) * | 2013-12-20 | 2018-02-13 | 温露 | Safflower endogenetic fungus, Thick many candies and its production and use |
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| CN105462850B (en) * | 2015-12-23 | 2019-03-01 | 广西大学 | Application of the sophora tonkinensis Gapnep endogenetic fungus SDTE-P in prevention and treatment notoginseng root rot |
| CN105462847B (en) * | 2015-12-23 | 2019-01-01 | 广西大学 | Application of the sophora tonkinensis Gapnep endogenetic fungus TRXY-46 in prevention and treatment notoginseng root rot |
| CN106376322B (en) * | 2016-08-29 | 2019-12-24 | 湖州市中心医院 | Saffron crocus seed ball propagation method |
| CN108069776A (en) * | 2017-11-22 | 2018-05-25 | 山东京青农业科技有限公司 | A kind of microbial-bacterial fertilizer for preventing potato wilt and its preparation method and application |
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