CN116426440B - Novel marine sulfur oxidizing bacteria strain and application thereof - Google Patents
Novel marine sulfur oxidizing bacteria strain and application thereof Download PDFInfo
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
- A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/48—Sulfur compounds
- B01D53/52—Hydrogen sulfide
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/345—Biological treatment of water, waste water, or sewage characterised by the microorganisms used for biological oxidation or reduction of sulfur compounds
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/40—Inorganic substances
- A62D2101/47—Inorganic substances containing oxygen, sulfur, selenium or tellurium, i.e. chalcogen
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- C02F2101/10—Inorganic compounds
- C02F2101/101—Sulfur compounds
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
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Abstract
The invention relates to the field of marine microorganism application, in particular to a marine sulfur oxidation strain and application thereof. The invention provides a strainPseudoruegeria diazotropha) And the application thereof, the strain is [ ], the strain isPseudoruegeria diazotropha) The preservation number is CGMCC No.1.16685, and the strain is a new marine strain. Experiments prove that the strain of the inventionPseudoruegeria diazotropha) Has stronger capability of rapidly oxidizing sulfide and thiosulfate ions, so that the catalyst can be used for removing sulfide and thiosulfate ions and has wide application prospect.
Description
Technical Field
The invention relates to the field of marine microorganism application, in particular to a marine sulfur oxidation strain and application thereof.
Background
Elemental sulfur is a nutritive element essential for biological growth and is widely availableIn nature, it exists in a variety of chemical forms including elemental sulfur, reduced sulfides, sulfates, and sulfur-containing organics. Common reducing sulfides include H 2 S, sulfide, thiosulfate (S) 2 O 3 2– ) And Sulfite (SO) 3 2– ) Etc. In the natural sulfur cycle, sulfur oxidation is an important component of the sulfur element bio-geochemical cycle, and generally refers to the process of oxidizing elemental sulfur or reduced sulfides by microorganisms. Sulfur Oxidizing Bacteria (SOB) are drivers of biological oxidation of elemental sulfur or reduced sulfides, and play an important role in the sulfur oxidation process. In nature, sulfur oxidizing bacteria are various, and sulfur oxidation related genes, enzymes and pathways are also various.
Industrial and agricultural production and life can produce and discharge a large amount of sulfides to the environment, resulting in air pollution, material corrosion and biological poisoning. SOB plays an important role in the treatment of pollutants such as wastewater, waste gas, solid waste and the like and the recovery of sulfur resources, and therefore SOB is applied to the problem of sulfide pollution generated in the fields of agriculture, environmental engineering, metallurgical industry and the like.
SOB is not only various, but also widely distributed, and found in various environments such as oceans, hot liquid ports, cold springs, soil, rivers, lakes and the like. SOB has been found to be a green sulfur bacterium, a purple non-sulfur bacterium, a colorless sulfur bacterium, etc., and other groups of SOB are among FirmicsAlicyclobacillusspp., in green non-sulfur bacteriaChloroflexus aurantiacusAnd AquificaeSulfurihydrogenibiumspp.
Since a large amount of novel SOB exists in the marine environment, the excavation of SOB resources in the marine environment is required to be increased, and the acquisition of more SOB is also helpful for discovering new sulfur-oxidizing genes, enzymes or paths and the like, so that strain resource guarantee is provided for pollution control, resource recovery and the like.
Disclosure of Invention
In view of this, the present invention provides marine sulfur oxidizing strains and uses thereof. Experiments prove that the strain HB172150 has strong capability of rapidly oxidizing sulfide and thiosulfate ions, so that the strain HB172150 can be used for removing sulfide and thiosulfate ions and has wide application prospect.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a strain with a preservation number of CGMCC No.1.16685.
In some embodiments of the invention, the strain is used in a form that includes one or more of its live bacteria, inactivated bacteria, fermentation broth, exosomes or metabolites thereof.
In some embodiments of the invention, the strain is cultured in 2216E medium at 28 ℃ for 48 hours, and the bacterial cells are observed by a transmission electron microscope and are elliptical (shown in figure 2); the growth on nutrient agar (Haibo organism, product number HB 0109), R2A agar (Haibo organism, product number HB 0167) and 2216E agar medium (Haibo organism, product number HB 0132) is good, clear colony is visible, the colony is elliptic, and the color is milky to yellowish; in D Ö bereiner agar medium (sucrose 10g, malic acid 5.0g, K) 2 HPO 4 ·H 2 O 0.1g，KH 2 PO 4 ·H 2 O 0.4g，MgSO 4 ·7H 2 O 0.2g，CaCl 2 0.02g，Na 2 MOO 4 ·H 2 O 0.002g，FeCl 3 0.01g,50% aged seawater to 1L, pH 7.0), the colony is elliptical and translucent in color.
In some embodiments of the invention, the strain may utilize mannitol but D-glucose, L-arabinose, D-mannose, N-acetyl-glucosamine, maltose, gluconate, decanoic acid, adipic acid, malic acid, citric acid, and phenylacetic acid cannot be utilized in the 12 carbon sources of API 20 NE (Biomerieux, cat No. 20050).
In some embodiments of the invention, the strain produces cytochrome oxidase, b-glucosidase, b-galactosidase, and nitrate reductase. Tryptophan hydrolase, arginine bishydrolase, urease and protease are not produced.
Some of the inventions haveIn embodiments, the strain is capable of degrading gelatin, acidifying glucose, and does not produce H 2 S and melanin.
In some specific embodiments of the invention, the strain NaCl concentration growth adaptation range is 2% -7%. The pH suitable for growth is 5-8.
The invention also provides a microbial agent, which comprises the strain.
The invention also provides the strain; or the application of the microbial agent in preparing any of the following products:
(I) Producing cytochrome oxidase, beta-glucosidase, beta-galactosidase, and/or nitrate reductase; and/or
(II) oxidizing sulfur-containing species; and/or
(III) sulfide removal; and/or
(IV) preventing and treating sulfide pollution.
In some embodiments of the invention, the sulfur-containing species comprises one or more of elemental sulfur, sulfate, or reduced sulfide.
In some embodiments of the invention, the sulfur species further comprises sulfur-containing organics.
In some embodiments of the invention, the reducing sulfide includes H 2 S, one or more of sulfide, thiosulfate or sulfite.
In some embodiments of the invention, the reducing sulfide comprises sodium sulfide or sodium thiosulfate.
The invention also provides a product comprising any of the following:
(I) The strain; and/or
(II) the microbial agent.
In some embodiments of the invention, the strain has desirable sulfide removal in sodium sulfide medium, and the removal rate of sulfide in the medium after 24h culture is 90% ± 3%; the method has the capability of oxidizing thiosulfate ions quickly in an SOB culture medium, and the removal rate of sodium thiosulfate in the culture medium after 24 hours of culture is 82% +/-4%.
The invention also provides an SOB enrichment medium, the mass ratio of the components of the SOB enrichment medium comprises NH 4 Cl：Na 2 S 2 O 3 ：K 2 HPO 4 ：MgSO 4 •7H 2 O：CaCl 2 •2H 2 O=0.5：8.0：0.5：0.2：0.1；
The SOB enrichment medium pH comprised 7.2.
The invention also provides a method for removing sulfides based on any of the following:
(I) The strain; and/or
(II) the microbial agent.
The invention also provides a high-throughput culture screening method of sulfur-oxidizing bacteria, which is based on the SOB enrichment medium screening of claim 9, and 16S rDNA sequence molecular identification to obtain sulfur-oxidizing bacteria;
the mass ratio of the components of the SOB enrichment medium comprises NH 4 Cl：Na 2 S 2 O 3 ：K 2 HPO 4 ：MgSO 4 •7H 2 O：CaCl 2 •2H 2 O=0.5：8.0：0.5：0.2：0.1；
The SOB enrichment medium pH comprised 7.2.
The invention includes, but is not limited to, achieving the following benefits:
the novel resource strain has ideal sulfide removal capacity in a sodium sulfide culture medium, and the removal rate of sulfide in the culture medium after 24h is 90% +/-3%; the method has the capability of oxidizing thiosulfate ions quickly in an SOB culture medium, and the removal rate of sodium thiosulfate in the culture medium after 24 hours of culture is 82% +/-4%. Experiments prove that the strain HB172150 has strong capability of rapidly oxidizing sulfide and thiosulfate ions, so that the strain HB172150 can be used for removing sulfide and thiosulfate ions and has wide application prospect.
Preservation of organisms
Biological material: HB172150 was deposited at the China general microbiological culture Collection center, the accession number of which is: the collection number of the microbiological institute of China academy of sciences is CGMCC No.1.16685, and the North Chen Xili No.1, 3 of the Chaoyang area of Beijing city.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below.
FIG. 1 shows the phylogenetic similarity comparison of strain HB 172150;
FIG. 2 shows a transmission electron micrograph (10000X) of strain HB172150 after 48h incubation in 2216E medium at 28 ℃.
Detailed Description
The invention discloses a marine sulfur oxidizing strain and application thereof, and a person skilled in the art can properly improve the process parameters by referring to the content of the marine sulfur oxidizing strain. It is expressly noted that all such similar substitutions and modifications will be apparent to those skilled in the art, and are deemed to be included in the present invention. While the methods and applications of this invention have been described in terms of preferred embodiments, it will be apparent to those skilled in the relevant art that variations and modifications can be made in the methods and applications described herein, and in the practice and application of the techniques of this invention, without departing from the spirit or scope of the invention.
In the marine sulfur oxidation strain and the application thereof, the raw materials and the reagents used in the marine sulfur oxidation strain can be purchased from the market.
The invention is further illustrated by the following examples:
example 1 enrichment and isolation of Marine Sulfur-oxidizing bacteria
The SOB enrichment medium is an inorganic medium prepared by taking sodium thiosulfate as a main energy source, and because the metabolic pathway of the sodium thiosulfate is similar to that of sulfide, microorganisms with ideal growth conditions often have better sulfide oxidizing capability when taking the sodium thiosulfate as the metabolic energy source.
Taking 5-10 cm sediment from Hainan Wenchang Gao Longwan mangrove, taking 500-g sediment, adding into sterilized 50% aged seawater with the same volume, and placing into glass beadsShaking for 15 min, and inoculating into SOB enrichment medium (main energy substance is Na 2 S 2 O 3 The carbon source and the nitrogen source are respectively CO 2 And NH 4 The specific formula of Cl is as follows: NH (NH) 4 Cl 0.5 g/L，Na 2 S 2 O 3 8.0 g/L，K 2 HPO 4 0.5 g/L，MgSO 4 •7H 2 O 0.2 g/L，CaCl 2 •2H 2 O0.1 g/L) was formulated with 50% aged seawater and the pH was adjusted to 7.2.Na (Na) 2 S 2 O 3 Dissolving in sterilized ultrapure water, filtering with 0.22 μm filter membrane, sterilizing other components at 121deg.C for 20 min, wherein MgSO 4 •7H 2 O and CaCl 2 •2H 2 O was sterilized separately and added to the medium.
To determine the optimal inoculum size, 2%, 5%, 10% and 20% inoculum sizes were inoculated into 96-well plates containing SOB liquid medium, and enrichment cultured at 28℃and 150 r/min for 7 days, OD600 of the microorganisms was measured, and the optimal inoculum size of sulfur-oxidizing bacteria was determined, and the results showed that 10% inoculum size was optimal. OD600 and pH were observed daily in the medium during the culture. The medium was transferred to freshly prepared SOB broth at 20% volume each time the OD600 was above 1.0 or the medium pH was below 3.0.
EXAMPLE 2 isolation of Marine Sulfur-oxidizing bacteria
After 5 times of transfer of the sulfur oxidizing bacteria enriched culture solution in example 1, the enriched sulfur oxidizing bacteria were inoculated with 10 -1 ，10 -2 ，10 -3 ，10 -4 Diluted at double concentration and spread evenly onto 2216E medium solid plates. And (3) placing the solid culture medium into a constant temperature incubator, and culturing for 2-3 days at the temperature of 28 ℃. After bacterial colonies grow out on the solid plate, picking the bacterial colonies, re-inoculating the bacterial colonies on 2216E solid culture medium, and continuously streaking and culturing for a plurality of times until purified bacterial strains are obtained.
Example 3 high throughput screening of marine sulfur oxidizing bacteria
The purified strain obtained in example 2 was inoculated back into SOB medium using sodium thiosulfate as a substrate, and the medium became turbid after 24 hours. The specific operation is as follows:
250. Mu.L of SOB culture solution using sodium thiosulfate as a substrate is added into a 96-well plate, the separated strains are inoculated into the culture medium in an inoculation amount of 25. Mu.L, a group of parallel strains are prepared by each inoculation, and 25. Mu.L of sterilized phosphate buffer solution is added into the remaining 2 holes to serve as a blank control group. The OD600 value in each well on a 96-well plate was measured with a microplate reader before the start of culture. The OD600 was measured by incubating the 96-well plates at 28℃for 48 h. 13 strains were co-selected, and after 24 hours of culture, SOB culture solution using sodium thiosulfate as a substrate became turbid (OD 600 values before and after the culture of 13 strains are shown in Table 1).
TABLE 1 OD600 values before and after culture of 13 strains
Example 4 identification of bacterial strains of marine sulfur oxidizing bacteria
2 mu LHB172150 bacterial liquid is taken out in a centrifuge tube of 1.5 mL, 30 mu L of sterile water is added, the mixture is evenly mixed and then is rapidly centrifuged to prevent the liquid from hanging on the wall, and the centrifuge tube is placed in a metal bath at 100 ℃ for incubation for 10 min.12000 And centrifuging at rpm for 1 min, taking 2 mu L of supernatant as template DNA, and amplifying and sequencing the 16S rRNA of the strain to be tested. The 16S rDNA sequences of the purified strains were PCR amplified using bacterial universal primers 27f (5 '-AGAGTTTGAT CMTGCCTCAG-3', SEQ ID No. 1) and 1492r (5 '-TACGGYTACCTTGTTACGACTT-3', SEQ ID No. 2), and the 16S rDNA sequences were submitted to EzBioCloud (http:// www.ezbiocloud.net /) for BLAST alignment analysis.
The 13 strains are identified by 16S rDNA sequence molecules, wherein the strain HB172150 is a model strain with the closest relationship with the genetic relationshipPseudoruegeria marinistellaeSF-16 T The 16S rDNA homology is 96.33%, and the similarity between the other 12 strains and the mode strain with the closest relatedness is more than 99% (see Table 2 for details). According to the reference, the strain with the 16S rDNA homology less than 97% with the mode strain of the recent relationship is a new strain, so that only the strain HB172150 is used as a target strain for subsequent research, and a new strain resource is provided for developing a sulfur oxidation functional preparation.
TABLE 2 model strains with closest affinity for 13 strains and their similarity
A phylogenetic tree (shown in FIG. 1) was constructed by the proximity ligation method (Neighbor-Joining) using MEGA 7.0 according to the gene sequence of 16S rRNA of strain HB172150 (shown in SEQ ID No. 3) and the closely related model strain.
The results of the physiological and biochemical experiments in example 5 below further indicate that the bacterium belongs to ParacoceceaePseudoruegeriaBelongs to a new species with sulfur oxidation function. The strain HB172150 is preserved in China general microbiological culture Collection center (CGMCC) for 9 months and 9 days in 2021, the preservation address is 1 # 3 of North West Lu 1 of the Korean area of Beijing, the post code is 100101, and the preservation number is CGMCC 1.16685.
The 16S rDNA sequence of strain HB172150 is:
CTTGAGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGCACTTTCGGGTGAGCGGCGGACGGGTGAGTAACGCGTGGGAATATGCCCAAAGGTAGGGAATAGCCTCTGGAAACGGAGAGTAATACCCTATGTGCCCTACGGGGGAAAGATTTATCGCCTTTGGATTAGCCCGCGTTGGATTAGGTAGTTGGTGGGGTAATGGCCTACCAAGCCGACGATCCATAGCTGGTTTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATCTTAGACAATGGGGGAAACCCTGATCTAGCCATGCCGCGTGAGTGACGAAGGCCTTAGGGTCGTAAAGCTCTTTCGCTGGGGAAGATAATGACGGTACCCAGTAAAGAAACCCCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGGGTTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTAGGCGGACTATTAAGTCAGGGGTGAAATCCCGCGGCTCAACCGCGGAACTGCCTTTGATACTGGTAGTCTAGAGTTCTGGAGAGGTGAGTGGAATTCCGAGTGTAGAGGTGAAATTCGTAGATATTCGGAGGAACACCAGTGGCGAAGGCGGCTCACTGGCCAGATACTGACGCTGAGGTGCGAAAGTGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACACCGTAAACGATGAATGCCAGACGTCGGCAAGCATGCTTGTCGGTGTCACACCTAACGGATTAAGCATTCCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGCAGAACCTTACCAACCCTTGACATCCTGATCGCGGTTACCAGAGATGGTTTCCTTCAGTTCGGCTGGATCAGTGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTCGGTTAAGTCCGGCAACGAGCGCAACCCACACCCTTAGTTGCCAGCAGTTCGGCTGGGCACTCTAGGGGAACTGCCCGTGATAAGCGGGAGGAAGGTGTGGATGACGTCAAGTCCTCATGGCCCTTACGGGTTGGGCTACACACGTGCTACAATGGTGGTGACAGTGGGTTAATCCCCAAAAGCCATCTCAGTTCGGATTGTCGTCTGCAACTCGACGGCATGAAGTCGGAATCGCTAGTAATCGCGTAACAGCATGACGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTTGGGTTTACCCGAAGACGGTGCGCCAACCTTTATAGGGGGCAGCTGGCCACGGTAAGCTCAGCGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTT（SEQ ID No.3）
example 5 cultivation and microbial Properties of Marine Sulfur Oxidation Strain HB172150
1) Morphology and culture Properties
After strain HB172150 was cultured in 2216E medium at 28℃for 48 hours, the cells were observed by a transmission electron microscope and were oval (see FIG. 2).
Strain HB172150 grows well on nutrient agar (sepiole, cat# HB 0109), R2A agar (sepiole, cat# HB 0167) and 2216E agar medium (sepiole, cat# HB 0132) and shows clear colonies, oval, milky to yellowish in color; in D Ö bereiner agar medium (sucrose 10g, malic acid 5.0g, K) 2 HPO 4 ·H 2 O 0.1g，KH 2 PO 4 ·H 2 O 0.4g，MgSO 4 ·7H 2 O 0.2g，CaCl 2 0.02g，Na 2 MOO 4 ·H 2 O 0.002g，FeCl 3 0.01g,50% aged seawater to 1L, pH 7.0), the colony is elliptical and translucent in color. The specific characteristics are shown in Table 3.
TABLE 3 culture characteristics of strain HB172150
2) Physiological and biochemical characteristics
Strain HB172150 mannitol can be used in 12 carbon sources of API 20 NE (Biomerieux, cat. No. 20050), but D-glucose, L-arabinose, D-mannose, N-acetyl-glucosamine, maltose, gluconate, decanoic acid, adipic acid,Malic acid, citric acid and phenylacetic acid cannot be utilized. Producing cytochrome oxidase, beta-glucosidase, beta-galactosidase, and nitrate reductase. Tryptophan hydrolase, arginine bishydrolase, urease and protease are not produced. Can not degrade gelatin, acidify glucose, and does not produce H 2 S and melanin, see in particular Table 4.
The strain growth pH (4-12 in 1 unit increments) and tolerance to NaCl (0-12% in 1% (w/v)) were tested on 2216E medium. The result shows that the pH of the strain suitable for growth is 5-8, and the growth adaptation range of NaCl concentration is 2% -7%.
TABLE 4 physiological and biochemical characteristics of strain HB172150
Effect example 1 Marine sulfur Oxidation Strain HB172150 Effect on sodium sulfide removal
The strain stored on the solid 2216E slant was inoculated into 2216E medium, and cultured with shaking (28 ℃ C., 120 r/min) for 24h as seed solution, and the seed solution was inoculated into 50 mL sodium sulfide medium (Na 2 S 0.1g/L，NH 4 Cl 0.5 g/L，K 2 HPO 4 0.5 g/L, pH to 7.2), shaking culture at 28deg.C with a shaking table of 120 r/min for 24h, according to national environmental protection agency standard "method for measuring iodine amount of Water quality sulfide" (standard number: HJ/T60-2000) and the removal rate was calculated by measuring the concentration of sodium sulfide by the iodometry method. The strain used in the experiment was new strain HB172150 compared with other strains obtained in the laboratory (selection of strains growing faster) (Table 5), and the results show that strain HB172150 has ideal sulfide removal capacity in sodium sulfide medium, and the removal rate of sulfide in the medium after 24% h cultivation is 90% + -3%.
TABLE 5 removal of sulfide in Medium after 24h Strain culture (%)
Effect example 2 Marine sulfur Oxidation Strain HB172150 oxidizing Capacity for sodium thiosulfate
Strain HB172150 was inoculated into 2216E liquid medium, cultured at 28 ℃ with shaking (120 r/min) for 24h, seed solution was inoculated into 50 mL sodium thiosulfate solution (final concentration 5 mmol/L) of SOB medium (final concentration of the remaining components is unchanged) at 10% of the inoculum size, shake culture was performed at 28 ℃ with shaking (120 r/min) for 24h, and the concentration of sodium thiosulfate was determined by an iodometric method according to the reference.
Strain HB172150 was compared to other laboratory-obtained strains (selection of faster growing strains) (table 6), and the results indicated that strain HB172150 had the ability to oxidize thiosulfate ions faster in the SOB medium and that the removal rate of sodium thiosulfate in the medium after 24h of culture was 82% ± 4%.
TABLE 6 removal of sodium thiosulfate from the Medium after 24h Strain culture (%)
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (9)
1. A bacterial strain is characterized in that the preservation number is CGMCC No.1.16685.
2. The strain of claim 1, wherein the form of use comprises one or more of a live bacterium, an inactivated bacterium, a fermentation broth, an exosome, or a metabolite thereof.
3. Microbial agent, characterized in that it comprises a strain according to any one of claims 1 to 2.
4. Use of a strain according to any one of claims 1 to 2 or a microbial agent according to claim 3 for the preparation of any one of the following products:
(I) For oxidizing sulfides;
(II) for sulfide removal;
the sulfide is thiosulfate.
5. The use according to claim 4, wherein said removal of sulfides comprises for controlling sulfide contamination.
6. The use according to claim 5, wherein the sulphide is sodium sulphide or sodium thiosulfate.
7. A product comprising any one of:
I. the strain of claim 1 or 2;
II. The microbial agent according to claim 3.
8. A method for removing sulfides, characterized by removing sulfides based on any one of the following:
I. the strain of claim 1 or 2;
II. A microbial agent according to claim 3;
the sulfide is thiosulfate.
9. The method of claim 7, wherein the sulfide is sodium sulfide or sodium thiosulfate.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103667096A (en) * | 2013-06-14 | 2014-03-26 | 江南大学 | Sulfur-oxidizing bacterium and method for removing sulfide by using sulfur-oxidizing bacterium |
| CN103966128A (en) * | 2014-04-30 | 2014-08-06 | 江南大学 | Thiobacillus neapolitanus and application of thiobacillus neapolitanus in biological desulfurization |
| KR101957208B1 (en) * | 2017-12-11 | 2019-07-04 | 한국생명공학연구원 | Pseudoruegeria sp. M32A2M strain having algicidal activity against Alexandrium tamarense and uses thereof |
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| CN103667096A (en) * | 2013-06-14 | 2014-03-26 | 江南大学 | Sulfur-oxidizing bacterium and method for removing sulfide by using sulfur-oxidizing bacterium |
| CN103966128A (en) * | 2014-04-30 | 2014-08-06 | 江南大学 | Thiobacillus neapolitanus and application of thiobacillus neapolitanus in biological desulfurization |
| KR101957208B1 (en) * | 2017-12-11 | 2019-07-04 | 한국생명공학연구원 | Pseudoruegeria sp. M32A2M strain having algicidal activity against Alexandrium tamarense and uses thereof |
Non-Patent Citations (1)
| Title |
|---|
| Pseudoruegeria limi sp. nov. isolated from mud flats in the Yellow Sea in Korea;Jae-Bong Lee 等;《Antonie Van Leeuwenhoek》;第105卷(第5期);第987-994页 * |
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