CN116406679A - Application of formula composition containing plant extract in plant growth regulating activity - Google Patents

Application of formula composition containing plant extract in plant growth regulating activity Download PDF

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Publication number
CN116406679A
CN116406679A CN202310294517.5A CN202310294517A CN116406679A CN 116406679 A CN116406679 A CN 116406679A CN 202310294517 A CN202310294517 A CN 202310294517A CN 116406679 A CN116406679 A CN 116406679A
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active ingredient
plant growth
growth regulating
plant
crops
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Inventor
冉兆晋
王仲民
甘伟江
周繁
崔顺艳
刘兴斌
魏广
许黄文
庞锐
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Guangxi Duodele Biotechnology Co ltd
Guangxi Academy of Sciences
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Guangxi Academy of Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00Plant growth regulators
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Pest Control & Pesticides (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Botany (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention provides the field of agriculture, and particularly relates to application of a formula combination containing plant extracts to plant growth regulating activity. The active components of the formula combination are an active component A and an active component B, wherein the active component A is persimmon tannin, and the active component B is chitosan oligosaccharide. Compared with a single dose, the plant growth regulating formula combination has more remarkable effect, can enhance the stress resistance of crops, promote the growth of the crops, increase the yield of the crops and improve the quality of the fruits of the crops, and can be used as a emasculation agent.

Description

Application of formula composition containing plant extract in plant growth regulating activity
Technical Field
The invention relates to the field of agriculture, in particular to application of a formula composition containing plant extracts to plant growth regulating activity.
Background
The plant growth regulator is a hormone substance which is widely applied to the agricultural production for regulating the growth and development of plants, can effectively regulate and control the growth and development of plants, enhance stress resistance, increase yield and improve quality, and has the characteristics of small dosage, quick response, less residue and strong pertinence. The effects on growth can be classified into a plant growth promoter, a plant growth inhibitor and a plant growth retardant, which are selected according to plant varieties and application purposes. Common growth promoters are indolepropionic acid, naphthylacetic acid, 6-benzyladenine; common growth inhibitors are triiodobenzoic acid, a juglandins; common growth retardants are uniconazole, paclobutrazol and chlormequat chloride. The research shows that when two or more plant growth regulators are used in a mixed mode or used in sequence, the effect is better than that of single application, and the regulation and control effects are better exerted; however, it is also possible that the use of two or more plant growth regulators in combination inhibits the growth of crops, resulting in lower crop yields or a yield improvement which is less than the effect of the use of a single dose, and therefore rational use of plant growth regulation is of paramount importance.
The preparation of excellent high-yield hybrid seeds by using the regulator is an important method for improving grain yield, the key problem of regulator breeding is to have an excellent regulator with high emasculation rate without affecting female organs, and the emasculation agent is an important agricultural production application of the regulator. The existing emasculation agents are few in variety, most of chemical synthesis products such as maleic hydrazide and the like, and green chemicals lack of the emasculation agents in the market are one main direction of the existing emasculation agent research.
In recent years, plant growth regulators are becoming more and more important, so that not only can agricultural cost be reduced, but also crop growth and yield can be improved, fruit quality and fertilizer utilization efficiency can be improved, soil environment can be protected, and diseases can be reduced.
Disclosure of Invention
The present invention aims to overcome the above-mentioned drawbacks of the prior art and to provide the use of a formulation combination comprising a plant extract for plant growth regulating activity.
The technical idea is as follows: the persimmon tannin extracted from green persimmon is used as an active ingredient, and is added with chitosan oligosaccharide according to a certain proportion to form a novel plant growth promoting regulator, so that the growth and development of plants can be promoted, the yield of the plants can be improved, the stress resistance of crops can be improved, and the quality of the crops can be improved.
And the technical idea is as follows: persimmon tannin extracted from green persimmon is used as an active ingredient, and is added with chitosan oligosaccharide according to a certain proportion to form a new green chemical plant emasculation agent, so that the persimmon tannin can be used for high-yield hybrid seed breeding.
The invention adopts the technical scheme that:
the formula combination of the plant growth regulating activity is characterized by comprising an active ingredient A and an active ingredient B, wherein the active ingredient A is persimmon tannin, the active ingredient B is chitosan oligosaccharide, and the weight ratio of the active ingredient A to the active ingredient B is 1-20:20-200.
In the technical scheme, the formula is used for promoting plant growth, and the weight ratio of the active ingredient A to the active ingredient B is as follows: 1-2.5:75-100.
Further, the concentration range of the active ingredient A is 0.1-0.25mg/L, and the concentration range of the active ingredient B is 7.5-10mg/L.
In the technical scheme, the formula is used for a plant emasculation agent, and the weight ratio of the active ingredient A to the active ingredient B is as follows: 1-3:1-10.
Further, the concentration range of the active ingredient A is 200mg-300mg/L, and the concentration range of the active ingredient B is 500mg-1000mg/L.
Further, the concentration of the active ingredient A is 200mg/L, and the concentration of the active ingredient B is 500mg/L.
In the above technical scheme, the active ingredient A is derived from persimmon tannin extracted from green persimmon.
And the technical idea is as follows: the plant growth regulator composition prepared by the method is applied to peppers and other crops such as peanuts, wheat, rice and potatoes.
Based on the technical thought, the technical scheme adopted by the invention is as follows:
the use of a formulation combination of plant growth regulating activity for promoting or regulating crop growth in the agricultural field or in a breeding emasculation agent.
In the technical scheme, the composition is applied to wheat, rice, peanut, potato or capsicum.
The invention has the beneficial effects that:
1. the active ingredients of the regulator are persimmon tannin and chitosan oligosaccharide, and the weight ratio is 1-20:20-200 has remarkable synergistic effect in the aspect of regulating or promoting plant growth, and has optimal effect when the concentration of chitosan oligosaccharide is 7.5mg/L and the concentration of persimmon tannin is 0.25mg/L, thus being capable of improving stress resistance and crop yield of crops, improving crop quality and improving economic and social benefits.
2. The active ingredients of the regulator are persimmon tannin and chitosan oligosaccharide, the regulator has remarkable synergistic effect in the aspect of inducing plant emasculation, and has optimal wheat emasculation rate and outcrossing setting rate when the concentration of persimmon tannin and chitosan oligosaccharide is 200mg/L+500mg/L, thereby greatly improving the breeding efficiency and promoting the agricultural production to improve the benefit.
3. Meanwhile, the use frequency and the use amount of the plant growth regulator are adjusted along with the crops, the growth environment and the climate temperature, and the production purpose can be achieved by using proper dosage.
4. The plant growth regulator is a natural product, meets the environmental-friendly industrial requirement, can be rapidly and naturally degraded in plant bodies or soil, and is not easy to generate residues in plant bodies and soil.
5. The plant growth regulator disclosed by the invention can promote the increase of chlorophyll content of plants, improve photosynthetic efficiency, be beneficial to accumulation of plant dry matters and further promote the increase of yield.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below.
Example 1
Active ingredients a and B were dissolved in water and formulated at different concentrations (table 1).
Table 1: persimmon tannin and chitosan oligosaccharide compound
Figure SMS_1
Application example: indoor potting test verification
Test object: gala small bell pepper (supplied by Harbin golden dragon agriculture Co., ltd.)
The test method comprises the following steps: the experiments were carried out in a glass greenhouse. Uniformly mixing turfy soil and sand according to a mass ratio of 1:1, loading the mixture into a 54 cm-27 cm-6 cm seedling tray, uniformly sowing seeds into the seedling tray, covering a soil layer with a thickness of about 0.5cm, watering from the bottom, and sprouting and seedling under the condition of abundant sunlight at 24-28 ℃. After 2 true leaves of the pepper plants grow, selecting pepper seedlings with consistent growth vigor, transplanting the pepper seedlings into a flowerpot, keeping the growth conditions of each pot seedling consistent, and applying a base fertilizer after seedling reviving, wherein the base fertilizer is conventional compound fertilizer 15-15-15, and the dosage is 600kg/hm 2 And (3) application. The first agent spraying was performed 15 days after transplanting, and the second agent spraying was performed 30 days later, the yield of capsicum was investigated in the fruiting period, each treatment was repeated 5 times, and the test results are shown in table 2.
The reagent is an experiment group reagent, a control group reagent and a blank; wherein the experimental group is a composition of chitosan oligosaccharide and persimmon tannin according to different compounding; the control group is chitosan oligosaccharide or persimmon tannin with different concentrations; the blank is clear water.
Table 2: measurement data of pepper pot experiment
Figure SMS_2
Figure SMS_3
Figure SMS_4
As shown in Table 2, the results of the potting experiments show that the foliar spray of chitosan oligosaccharide and persimmon tannin can promote the increase of the yield of the capsicum to different degrees, and the effect of the compound composition in the study is more obvious. As shown in the table, when the concentration of the sprayed chitosan oligosaccharide is 7.5-10mg/L and the concentration of the persimmon tannin is 0.1-0.25mg/L, the effect is most remarkable, and when the concentration of the sprayed chitosan oligosaccharide is 7.5mg/L and the concentration of the persimmon tannin is 0.25mg/L, the yield increase of the pepper is most remarkable, and the yield increase effect is higher than that of each single agent and other compound treatments, so that the synergistic effect is obvious. The plant growth regulator can obviously promote the accumulation and transportation of dry matters of plants, thereby promoting the improvement of the yield of the capsicum.
Example 2
Active ingredients a and B were dissolved in water and formulated at different concentrations (table 3).
Table 3: persimmon tannin and chitosan oligosaccharide compound
Figure SMS_5
Application example: wheat emasculation Activity test
Spraying the agent with the concentration shown in Table 3 on the wheat in the field 3-5 days before heading (the length of the main spike of the wheat is 1.0-1.2 cm), wherein each concentration sample is sprayed with 0.2m 2 The spraying amount was 100ml. The sterility rate in the emasculation effect adopts a bagging isolation method, 10 ears are taken from each cell of a female parent to be sleeved, and the number of the bagged ears is B; taking 10 ears which are subjected to emasculation treatment but are not covered with bags, wherein the grain number after threshing is W; the control area takes an equal amount of untreated ears, and the grain number after threshing is K. Relative sterility rate rmsr= (K-B)/kx 100%, relative outcrossing seed setting rate cfr= (W-B)/kx 100%.
Table 4: wheat relative sterility RMSR assay data
Figure SMS_6
From the data, it can be obtained that the persimmon tannin has better wheat emasculation effect compared with the chitosan oligosaccharide mixture, and has optimal effect when the concentration of persimmon tannin and chitosan oligosaccharide is 200mg/L+500 mg/L. The concentration is less than the value, and the emasculation rate is reduced; the concentration is higher than the value, and the castration rate is equivalent.
Table 5: CFR measurement data of wheat relative outcrossing fruiting rate
Figure SMS_7
Figure SMS_8
From the data, it can be obtained that the persimmon tannin has better wheat outcrossing fruiting rate effect compared with the chitosan oligosaccharide mixture, and has optimal effect when the concentration of persimmon tannin and chitosan oligosaccharide is 200mg/L+500 mg/L. The concentration is less than the value, the outcrossing setting rate is equivalent, but the emasculation rate is reduced; the concentration was higher than this, the emasculation rate was comparable, but the outcrossing setting rate was decreased.
As shown in the experiment, when the concentration of the persimmon tannin and the chitosan oligosaccharide is 200mg/L+500mg/L, the persimmon tannin and the chitosan oligosaccharide have optimal wheat emasculation rate and outcrossing setting rate, and the persimmon tannin and the chitosan oligosaccharide are green chemicals, so that the persimmon tannin and the chitosan oligosaccharide are expected to be applied to the agricultural field as green chemical emasculation agents.
The obtained water agent can also act on other crops, has obvious synergistic effect in the aspect of regulating or promoting plant growth, and can improve crop yield and crop quality; can promote the efficacy of chemical hybridization breeding and greatly improve the breeding efficiency. The water aqua can promote agricultural production to raise economic and social benefit.
The above-described embodiments of the present invention do not limit the scope of the present invention. Any of various other corresponding changes and modifications made according to the technical idea of the present invention should be included in the scope of the claims of the present invention.

Claims (9)

1. The formula combination of the plant growth regulating activity is characterized by comprising an active ingredient A and an active ingredient B, wherein the active ingredient A is persimmon tannin, the active ingredient B is chitosan oligosaccharide, and the weight ratio of the active ingredient A to the active ingredient B is 1-20:20-200.
2. A plant growth regulating active ingredient formulation according to claim 1, wherein the weight ratio of active ingredient a to active ingredient B is: 1-2.5:75-100.
3. A plant growth regulating active formulation combination according to claim 2, wherein: the concentration range of the active ingredient A is 0.1-0.25mg/L, and the concentration range of the active ingredient B is 7.5-10mg/L.
4. A plant growth regulating active ingredient formulation according to claim 1, wherein the weight ratio of active ingredient a to active ingredient B is: 1-3:1-10.
5. The combination of claim 4, wherein the plant growth regulating activity is: the concentration range of the active ingredient A is 200mg-300mg/L, and the concentration range of the active ingredient B is 500mg-1000mg/L.
6. The combination of claim 4, wherein the plant growth regulating activity is: the concentration range of the active ingredient A is 200mg/L, and the concentration range of the active ingredient B is 500mg/L.
7. The combination of claim 1, wherein the active ingredient a is derived from persimmon tannin extracted from green persimmon.
8. The use of a formulation combination of plant growth regulating activity for promoting or regulating crop growth in the agricultural field or in a breeding emasculation agent.
9. The use according to claim 8, wherein the composition is applied to wheat, rice, peanut, potato, or pepper.
CN202310294517.5A 2023-03-24 2023-03-24 Application of formula composition containing plant extract in plant growth regulating activity Pending CN116406679A (en)

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