CN105557689A - Bacillus wettable powder containing brassinolide and preparation method thereof - Google Patents
Bacillus wettable powder containing brassinolide and preparation method thereof Download PDFInfo
- Publication number
- CN105557689A CN105557689A CN201510951072.9A CN201510951072A CN105557689A CN 105557689 A CN105557689 A CN 105557689A CN 201510951072 A CN201510951072 A CN 201510951072A CN 105557689 A CN105557689 A CN 105557689A
- Authority
- CN
- China
- Prior art keywords
- bacillus
- brassin lactones
- wettable powder
- powder containing
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000193830 Bacillus <bacterium> Species 0.000 title claims abstract description 68
- 239000004563 wettable powder Substances 0.000 title claims abstract description 39
- 238000002360 preparation method Methods 0.000 title claims abstract description 26
- IXVMHGVQKLDRKH-VRESXRICSA-N Brassinolide Natural products O=C1OC[C@@H]2[C@@H]3[C@@](C)([C@H]([C@@H]([C@@H](O)[C@H](O)[C@H](C(C)C)C)C)CC3)CC[C@@H]2[C@]2(C)[C@@H]1C[C@H](O)[C@H](O)C2 IXVMHGVQKLDRKH-VRESXRICSA-N 0.000 title abstract description 15
- IXVMHGVQKLDRKH-KNBKMWSGSA-N brassinolide Chemical compound C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@@H](O)[C@H](O)[C@@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-KNBKMWSGSA-N 0.000 title abstract description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000000843 powder Substances 0.000 claims abstract description 22
- 240000007594 Oryza sativa Species 0.000 claims abstract description 21
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 21
- 235000009566 rice Nutrition 0.000 claims abstract description 21
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims abstract description 16
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 12
- 229920000609 methyl cellulose Polymers 0.000 claims abstract description 12
- 239000001923 methylcellulose Substances 0.000 claims abstract description 12
- 235000020183 skimmed milk Nutrition 0.000 claims abstract description 12
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- 244000061176 Nicotiana tabacum Species 0.000 claims abstract 2
- 150000002596 lactones Chemical class 0.000 claims description 78
- 239000007788 liquid Substances 0.000 claims description 35
- 239000002609 medium Substances 0.000 claims description 21
- 239000000203 mixture Substances 0.000 claims description 17
- 241001037822 Bacillus bacterium Species 0.000 claims description 16
- 238000009736 wetting Methods 0.000 claims description 14
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims description 12
- -1 polyoxyethylene nonylphenol Polymers 0.000 claims description 12
- RNMDNPCBIKJCQP-UHFFFAOYSA-N 5-nonyl-7-oxabicyclo[4.1.0]hepta-1,3,5-trien-2-ol Chemical compound C(CCCCCCCC)C1=C2C(=C(C=C1)O)O2 RNMDNPCBIKJCQP-UHFFFAOYSA-N 0.000 claims description 11
- 241000726221 Gemma Species 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 230000001954 sterilising effect Effects 0.000 claims description 9
- 244000063299 Bacillus subtilis Species 0.000 claims description 8
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 230000001580 bacterial effect Effects 0.000 claims description 8
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- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 4
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- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 2
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- GVGUFUZHNYFZLC-UHFFFAOYSA-N dodecyl benzenesulfonate;sodium Chemical compound [Na].CCCCCCCCCCCCOS(=O)(=O)C1=CC=CC=C1 GVGUFUZHNYFZLC-UHFFFAOYSA-N 0.000 abstract 1
- SNQQPOLDUKLAAF-UHFFFAOYSA-N nonylphenol Chemical compound CCCCCCCCCC1=CC=CC=C1O SNQQPOLDUKLAAF-UHFFFAOYSA-N 0.000 abstract 1
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- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
- A01N25/14—Powders or granules wettable
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Toxicology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses bacillus wettable powder containing brassinolide and a preparation method thereof. The bacillus wettable powder containing brassinolide is mainly prepared from brassinolide, a bacillus solution, nonyl phenol polyoxyethylene ether, ethanol, skim milk powder, methylcellulose, D425, sodium dodecyl benzene sulfonate and kieselguhr. The brassinolide is capable of improving the activity of plant based enzymes, improving the seed vigor, promoting early development, enhancing the photosynthetic efficiency, increasing the crop yield, improving the crop quality, improving the cold resistance, drought resistance, waterlogging resistance and saline-alkaline tolerance of crops, improving the stress tolerance, and alleviating pesticide phytotoxicity and chemical pollution phytotoxicity, and is capable of resisting hazards of multiple types of virus, bacteria and fungus and enhancing the crop disease resistance. The bacillus is capable of directly restraining or killing multiple types of plant diseases. The wettable powder is safe, environmentally friendly and efficient, is stable in production process, and can be widely applied to crops such as rice, flue-cured tobacco, corn, wheat, stawberries, watermelons, hot pepper, tomatoes and cucumbers.
Description
Technical field
The present invention relates to microbial manure and field of biological pesticide, be specifically related to a kind of bacillus wettable powder containing brassin lactones and preparation method thereof.
Background technology
The people such as U.S. agronomist J.W.Mitchell studied in 1970 and are extracted and obtain a kind of material that can significantly promote bean seedlings to grow from rape pollen, name as rape element, also known as brassin (Brassin), take sterol as the plant endogenous steroid bioactivator of skeleton, Britain N.B.Mandava equals 1978 by after plain for rape refining, and obtains having highly active crystal.1979, Grove etc. determined that its chemical constitution belongs to sterol lactone, therefore called after brassin lactones (Brassionlide).Thereafter, from other plants, be extracted again tens kinds there is bioactive brassin steroid material, be generically and collectively referred to as brassin steroids (Brassinosteroids, be called for short BRs), wherein, the strongest with the physiologically active of brassin lactones, be considered to a kind of new plant hormone.The discovery of BR class material to be called as in plant physiology research a new milestone, being be known as highly active efficient, wide spectrum, nontoxic plant growth regulator in the world, is also a kind of new plant growth regulator that current China development highly efficient farming to provide greater and better yields and the ecological agriculture have vigor and vitality most.
Its primary efficacy of brassin lactones is the activity, the raising seed vitality that improve enzyme, promotes early development; Strengthen photosynthetic efficiency, improve crop yield, improve crop quality; Raising crop is cold-resistant, drought-enduring, waterlogging, Salt And Alkali Tolerance ability, improves anti-adversity ability; Alleviate pesticide injury and chemical contamination poisoning, obvious to herbicide damage detoxications such as symetryne, butachlor, metsulfuron-methyl, ethametsulfuron, glyphosates; The harm of anti-multiple virus, bacterium, fungi, and strengthen crop disease-resistant ability.Brassin lactones, since it is found, has had preliminary cognition to the understanding of its structure, biosynthesis and metabolism approach, physiological action and mechanism, and these achievements in research effectively can be promoted the use of aborning for it and lay a good foundation.Brassin lactones is the plant growth regulator type of at present state-of-the art wide spectrum, efficient, safety, strong stress resistance in the world, nearly 40 kinds of like derivatives are found at present, what wherein physiologically active was higher, with practical value about have four, five kind, and achieve Prof. Du Yucang, lipid material in low cost, extensive, suitability for industrialized production brassin is become a reality.The use of brassin lactones, shows stable effect of increasing production, and to environment, plant, person poultry safety, input-output ratio is considerable, cereal crops more than 1: 10, economic crops more than 1: 20.Test through 6 years 10 multiclass crops proves, its scope of application is wide and have no adverse effects to crops.Now all kinds of crops such as cotton, grain, oil plant, fruit, vegetables, tobacco, sugarcane, flowers are widely used in.Brassin lactones abroad a lot of application study shows, its biologically active is far better than other plant hormone as growth hormone, gibberellin, the basic element of cell division.It is absorbed by plants, and can promote that plant cell division extends and regulate body physiological function, strengthen metabolism, promote plant growth, increase yield, improve quality, promote root system development, strengthen photosynthesis, improves crop drought resistance, cold-resistant, disease-resistant etc.
The people such as U.S. Thompson, in field trial, have carried out foliage-spray with the table brassinolide solution of 0.01ppm concentration to various crop, have achieved good effect of increasing production.Rate of growth: paddy rice 15%, Pueraria lobota 30%, radish 15%, potato 25%, Kidney bean and green pepper 6% ~ 7%, tomato 20% ~ 56%.At present, apply for a large amount of agricultural application patents abroad, as special preparations such as paddy rice, corn, soybean, potato, radish, potato class, melon, fruit and flowers.Show thus, brassin lactones steps into the practical stage by the trial period in application agriculturally.China has also carried out a series of brassin lactones simulation experiments, as Shanghai plant physiology research institute of the Chinese Academy of Sciences, Shanghai institute of materia medica cooperate with Japan, in Shanghai Suburbs and Henan Province, simulation experiments is carried out to crops such as wheat, corn, watermelon, cucumber, flue-cured tobacco, grape, paddy rice, result shows, concentration is the brassin lactones parahormone of 0.01 ~ 0.lppm, can make wheat increase yield 10%, corn yield increasing 9.8% ~ 18.4%, cucumber production promoting 30%, increasing yield of water melons 20%.
Research finds, the former medicine of brassin lactones and multiple common fungicide, chemical fertilizer, and plant growth regulator mixture is applied, and has significant synergistic effect and additive effect, in most of the cases, can improve fertilizer efficiency and bactericide effect of chemical fertilizer, reduces pesticide injury; Due to the various obvious effect of brassin lactones in plantation, but single auximone still has certain limitation, because its brassin lactones compounded technology also has more people to study, if Xu Xuesong is the plant growth regulator disclosing a kind of chlormequat and brassinolide mixture in 2009102273591 in the patent No.; Zhang Wei is disclose a kind of plant growth regualting composition containing diethyl aminoethyl hexanoate and brassin lactones in 201210033990X in the patent No.; Gou osmanthus English discloses a kind of plant growth regulator containing benayl aminopurine and brassin lactones in the patent No. 2010102019998; Gou osmanthus English discloses a kind of plant growth regulator containing gibberellin and brassin lactones in the patent No. 2010102018302; Zhi Huyu etc. disclose a kind of composition containing alga fertilizer and brassin lactones in the patent No. 2012103277055.But yet there are no brassin lactones and the composite technology of bacillus is open.
Bacillus is easily separated and obtains from soil and plant, can produce gemma that is heat-resisting, resistance, have significant Biocontrol Potential, is beneficial to the production of biocontrol fungicide, formulation and survives in the environment, surely grows and breeding.At present, bacillus for controlling plant diseases mainly contains (WichitraL, 2008) such as bacillus subtilis (Bacillussubtilis), bacillus laterosporus (B.1aterosporus), bacillus cereus (B.cereus), bacillus licheniformis (B.licheniformis), bacillus thuringiensis (B.thuringiensis), Paenibacillus polymyxa (Paenibacilluspolymyxa), bacillus pumilus (B.pumilus).
The fungus and bacterium disease of the Bacillus strain that the rhizosphere soil from crop now reported, root table, plant and blade separate to different plant has preventive and therapeutic effect, as the rice sheath blight disease in cereal crops, rice blast, wheat sharp eyespot, beans root rot, Rice blast (Mu Changqing, 2006), (Zhang Xia such as take-all, 2007), cladosporium leaf and fruit mould of tomato in vegetable disease, cucumber fusarium axysporum, cucumber downy mildew, grey mould of egg plant, eggplant powdery mildew, capsicum epidemic disease, tomato banded sclerotial blight etc. (LiuY, 2011).Bacillus also can control the multiple epidemic disease of adopting rear fruit, as Apple Mould Core, nectarine brown rot, Penicillium italicum, grey mould fruit rot of strawberry, powdery mildew of strawberry, banana blight, banana crown rot, banana anthracnose, apple pear penicilliosis, apple pear black spot, apple pear canker and Jinhua Pear fruit rot.In addition, bacillus also has good preventive and therapeutic effect (Zhang Cheng economizes, 2008) to dothiorella gregaria, rot, black spot and anthracnose, tea zonate spot, anthracnose of tobacco, balck shank, root rot and cotton seedling blight etc.
Although many with the microbial pesticide product that bacillus is main preparation, but all things considered, also exist to comprise research and development and produce and disconnect, formulation difficulty, constant product quality is poor, production technology falls behind, homogeneity of product is not high, manufacturing enterprise's small scale, the problem that application difficulty is large, except a little, application technology problem is also comprised with the problem that other biological prevention and control agent is similar, when carrying out disease control as utilized these biological prevention and control agents, consider the impact of environmental factor on preparation effect, temperature in environment, moisture, PH, bactericide, metal ion, other microorganism etc. all can have an impact to the application effect of microbial inoculum.Therefore, compared with chemical bactericide, its result of use is unstable, cannot accomplish the effect of getting instant result.Can be absorbed by plants if add in bacillus, can promote that plant cell division extends and regulate body physiological function, strengthen metabolism, promote plant growth, increase yield, improve quality, promote root system development, strengthen photosynthesis, the brassin lactones that raising crop drought resistance, cold-resistant, disease-resistant etc. acts on, then can see effect fast, and effect is more remarkable.
Due to this kind of high-efficiency broad spectrum of brassin lactones, nontoxic high-tech novel plant growth regulation, with there is broad-spectrum antiseptic, the bacillus category fungicide compounding of plurality of plant diseases can be prevented and treated, effectively can reduce corps diseases, strengthen plant autoimmunity, agricultural product will be conducive to meet the needs of the market, and dominate the market, become growth of agricultural efficiency, an effective way of increasing peasant income, should have broad prospects.
Summary of the invention
The object of this invention is to provide a kind of bacillus wettable powder containing brassin lactones and preparation method thereof; primarily of brassin lactones; bacillus bacterium liquid; emulsifier: polyoxyethylene nonylphenol ether, solvent: ethanol, gemma protectant: skim milk powder; methylcellulose; dispersant: D425, wetting agent: neopelex, carrier: diatomite forms.Brassin lactones in the present invention can improve activity, the raising seed vitality of phytoenzyme, promotes early development; Strengthen photosynthetic efficiency, improve crop yield, improve crop quality; Raising crop is cold-resistant, drought-enduring, waterlogging, Salt And Alkali Tolerance ability, improves anti-adversity ability; Alleviate pesticide injury and chemical contamination poisoning, obvious to herbicide damage detoxications such as symetryne, butachlor, metsulfuron-methyl, ethametsulfuron, glyphosates; The harm of anti-multiple virus, bacterium, fungi, and strengthen crop disease-resistant ability; Bacillus in the present invention can directly suppress various plants multiple diseases or kill; Wetting powder of the present invention mainly has promotion crop production, the effect of increase yield and raising quality, this wetting powder safety, environmental protection, efficiently, stable processing technique, can extensively in paddy rice, flue-cured tobacco, corn, wheat, strawberry, watermelon, capsicum, tomato, the application on the crops such as cucumber.
To achieve these goals, the technical solution used in the present invention is: a kind of bacillus wettable powder containing brassin lactones, it is characterized in that, each composition part of institute's wetting powder is by weight: brassin lactones 0.001-0.01 part, bacillus bacterium liquid 100-200 part, emulsifier: polyoxyethylene nonylphenol ether 0.01-0.1 part, solvent: ethanol 0.15-1.5 part, gemma protectant: skim milk powder 2-10 part, methylcellulose 1-5 part, dispersant: D4252-12 part, wetting agent: neopelex 4-20 part, carrier: diatomite 50-100 part forms.
In order to achieve the above object, a kind of preparation method of the bacillus wettable powder containing brassin lactones, is characterized in that, the bacillus wettable powder of preparation containing brassin lactones comprises following steps:
, bacillus bacterium liquid preparation
Take out culture presevation pipe, draw flat board with LB solid culture medium and recover, cultivate 48 hours for 37 DEG C.Under flat board in the single colony inoculation of picking 50 milliliters of LB medium, in incubator, 37 DEG C shake cultivation 24 hours.Seed adopts the inoculum concentration of 5%, and be seeded to the bacillus liquid medium in the large triangular flask of 5L, liquid amount is 2L, and 20-28 hour is cultivated in 37 DEG C of concussions, and detect its bacterial concentration, spore content, more than 8,000,000,000 CFU//mL, namely can be used as bacillus bacterium liquid.Wherein said LB medium: peptone 5g, beef extract 3g, sodium chloride 5g, water 1000mL, pH7.2, add agar 2% in solid culture medium.Wherein said bacillus liquid medium: glucose 10g/L, corn flour 18g/L, wheat bran 25g/L, fish meal 5g/L, magnesium sulfate 0.5g/L, manganese sulphate 0.5g/L, sodium dihydrogen phosphate 0.5g/L, sodium hydrogen phosphate 2.3g/L, pH7.2.The condition of each medium sterilization is: 0.10-0.15MPa, 121 DEG C of sterilizings 30 minutes;
, brassin lactones pretreatment
First brassin lactones 0.001-0.01 part is joined in ethanol 0.15-1.5 part and dissolves, then add polyoxyethylene nonylphenol ether 0.01-0.1 part to it, mixing brassin lactones solution;
, by step
the brassin lactones solution of preparation is added to step
in 100-200 part bacillus bacterium liquid of preparation, then to adding skim milk powder 2-10 part successively, methylcellulose 1-5 part, D4252-12 part, neopelex 4-20 part, diatomite 50-100 part, spraying dry after mixing, water content is lower than 5%, and air-flow crushing crosses 400 mesh sieves, detect not low 10,000,000,000 CFU/g of spore content, suspensibility is not less than 80%, and wetting time, not higher than 70s, is the bacillus wettable powder containing brassin lactones.
Wherein, a kind of bacillus wettable powder containing brassin lactones; best each composition part is by weight: brassin lactones 0.005 part, bacillus bacterium liquid 150 parts, emulsifier: polyoxyethylene nonylphenol ether 0.05 part; solvent: ethanol 0.75 part; gemma protectant: skim milk powder 5 parts, methylcellulose 2 parts, dispersant: D4253 part; wetting agent: neopelex 5 parts, carrier: 70 parts, diatomite composition.
Wherein, described bacillus bacterium liquid is by bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens, Paenibacillus polymyxa, bacillus megaterium, bacillus cereus, bacillus laterosporus, bacillus marinus, bacillus firmus, atrophy bacillus, methyl type bacillus, waxy Bacillus, not Kazakhstan bacillus, Bacillus mucilaginosus, Bacillus cercus, bacillus pumilus, bafillus natto, one or more mixtures in bacillus coagulans.
Wherein, the content of described brassin lactones is not less than 90%.
Wherein, described a kind of gemma wetting powder containing brassin lactones in paddy rice, flue-cured tobacco, corn, wheat, strawberry, watermelon, capsicum, tomato, the application on the crops such as cucumber.
Wherein, the method for described application is for doubly having carried out foliage-spray or having filled with root by wettable powder dilution agent 500-2000.
The invention has the beneficial effects as follows: the bacillus of the bacillus wettable powder in the present invention both containing the various plant disease of control, again containing brassin lactones plant growth regulator, there is nutrition adjustment, the multi-efficiencies such as sterilization, significantly can promote plant growth, raising output, oil recovery enhancement, raising crops self are cold-resistant, the ability of drought resisting.The gemma shelf-life durations of wetting powder of the present invention is long, stable processing technique.
Embodiment
Be described further with embodiment below, but the present invention is not limited to these examples.
Embodiment 1
A kind of bacillus wettable powder containing brassin lactones, best each composition part is by weight: brassin lactones 0.005 part, bacillus amyloliquefaciens bacterium liquid 150 parts, emulsifier: polyoxyethylene nonylphenol ether 0.05 part, solvent: ethanol 0.75 part, gemma protectant: skim milk powder 5 parts, methylcellulose 2 parts, dispersant: D4253 part, wetting agent: neopelex 5 parts, carrier: 70 parts, diatomite composition;
The bacillus wettable powder of preparation containing brassin lactones comprises following steps:
, bacillus bacterium liquid preparation
Take out bacillus amyloliquefaciens culture presevation pipe, draw flat board with LB solid culture medium and recover, cultivate 48 hours for 37 DEG C.Under flat board in the single colony inoculation of picking 50 milliliters of LB medium, in incubator, 37 DEG C shake cultivation 24 hours.Seed adopts the inoculum concentration of 5%, and be seeded to the bacillus liquid medium in the large triangular flask of 5L, liquid amount is 2L, and 20-28 hour is cultivated in 37 DEG C of concussions, and detect its bacterial concentration, spore content is 9,200,000,000 CFU/mL, namely can be used as bacillus amyloliquefaciens bacterium liquid.Wherein said LB medium: peptone 5g, beef extract 3g, sodium chloride 5g, water 1000mL, pH7.2, add agar 2% in solid culture medium.Wherein said bacillus liquid medium: glucose 10g/L, corn flour 18g/L, wheat bran 25g/L, fish meal 5g/L, magnesium sulfate 0.5g/L, manganese sulphate 0.5g/L, sodium dihydrogen phosphate 0.5g/L, sodium hydrogen phosphate 2.3g/L, pH7.2.The condition of each medium sterilization is: 0.10-0.15MPa, 121 DEG C of sterilizings 30 minutes;
, brassin lactones pretreatment
First brassin lactones 0.005 part is joined in ethanol 0.75 part and dissolve, then add polyoxyethylene nonylphenol ether 0.05 part to it, mixing brassin lactones solution;
, by step
the brassin lactones solution of preparation is added to step
in 150 parts of bacillus amyloliquefaciens bacterium liquid of preparation, then to adding skim milk powder 5 parts successively, methylcellulose 2 parts, D4253 part, neopelex 5 parts, 70 parts, diatomite, spraying dry after mixing, water content is lower than 5%, air-flow crushing crosses 400 mesh sieves, and detecting spore content is 11,900,000,000 CFU/g, and suspensibility is 89.2%, wetting time is 59s, is the bacillus amyloliquefaciens wetting powder containing brassin lactones.
Embodiment 2
Described in a kind of bacillus wettable powder containing brassin lactones, each composition part of wetting powder is by weight: brassin lactones 0.002 part, bacillus subtilis bacterium liquid 120 parts, emulsifier: polyoxyethylene nonylphenol ether 0.02 part, solvent: ethanol 0.3 part, gemma protectant: skim milk powder 3 parts, methylcellulose 2 parts, dispersant: D4253 part, wetting agent: neopelex 5 parts, carrier: 75 parts, diatomite composition;
The bacillus wettable powder of preparation containing brassin lactones comprises following steps:
, bacillus bacterium liquid preparation
Take out Bacillus subtilis strain preservation pipe, draw flat board with LB solid culture medium and recover, cultivate 48 hours for 37 DEG C.Under flat board in the single colony inoculation of picking 50 milliliters of LB medium, in incubator, 37 DEG C shake cultivation 24 hours.Seed adopts the inoculum concentration of 5%, and be seeded to the bacillus liquid medium in the large triangular flask of 5L, liquid amount is 2L, and 20-28 hour is cultivated in 37 DEG C of concussions, and detect its bacterial concentration, spore content is 8,800,000,000 CFU/mL, namely can be used as bacillus subtilis bacterium liquid.Wherein said LB medium: peptone 5g, beef extract 3g, sodium chloride 5g, water 1000mL, pH7.2, add agar 2% in solid culture medium.Wherein said bacillus liquid medium: glucose 10g/L, corn flour 18g/L, wheat bran 25g/L, fish meal 5g/L, magnesium sulfate 0.5g/L, manganese sulphate 0.5g/L, sodium dihydrogen phosphate 0.5g/L, sodium hydrogen phosphate 2.3g/L, pH7.2.The condition of each medium sterilization is: 0.10-0.15MPa, 121 DEG C of sterilizings 30 minutes;
, brassin lactones pretreatment
First brassin lactones 0.002 part is joined in ethanol 0.3 part and dissolve, then add polyoxyethylene nonylphenol ether 0.02 part to it, mixing brassin lactones solution;
, by step
the brassin lactones solution of preparation is added to step
in 120 parts of bacillus subtilis bacterium liquid of preparation, then to adding skim milk powder 3 parts successively, methylcellulose 2 parts, D4253 part, neopelex 5 parts, 75 parts, diatomite, spraying dry after mixing, water content is lower than 5%, air-flow crushing crosses 400 mesh sieves, and detecting spore content is 11,400,000,000 CFU/g, and suspensibility is 84.3%, wetting time is 66s, is the bacillus wettable powder containing brassin lactones.
The test effect of bacillus wettable powder in rice field of the brassinolide-containing that example 3 utilizes example 1 to produce
1, test is selected to carry out in rice high yield district semilate rice;
2, address choice is tested in peasant household of Long Zhu village one, beachhead town, Longhui County, Hunan Province farmland covered by contract;
3, test method: medicament usage and consumption: experimental group: by the bacillus amyloliquefaciens wettable powder dilution agent 1000 containing brassin lactones doubly rice seedling two leaf wholeheartedly to four leaf one heart stages, transplant after 5-7 days paddy rice period of seedling establishments and cut heading stage, select fine day dispenser.Bacillus group: the bacillus amyloliquefaciens with amount is diluted 1000 times and uses simultaneously; Brassin lactones group: 1000 times of uses will be diluted with amount brassin lactones; Blank group: the clear water of equivalent is sprayed.Often organizing usable floor area is 2 mu.Fertilization Level is purity nitrogen 20kg/667m2, P2O510kg/667m2, K2O20kg/667m2, base: tiller: fringe=5: 2: 3.Spray apparatus and select peasant's Versatile manual knapsack sprayer, the extermination of disease and insect pest and field management are a little more than Production of Large Fields.Brassin lactones group and blank group, owing to having rice blast in process of growth, utilize conventional medicine to carry out having prevented and treated twice.Other control measures are identical;
4, result of the test:
The rice yield of each experimental group of table 1
Because this bacillus amyloliquefaciens has control rice blast, the plurality of plant diseases such as banded sclerotial blight, use as can be seen from Table 1 in the paddy field of bacillus amyloliquefaciens and just rice blast does not occur, and use brassin lactones group have the generation of rice blast with the field of control group, be prevent and treat with conventional medicine.Use the field volume increase of the bacillus amyloliquefaciens wetting powder of brassinolide-containing at most, units increased in production reaches 24.9%, is used alone the volume increase that also there is 12-14% in brassin lactones and the field of separating shallow lake bacillus bacterium, illustrates that it is used alone and also has fertilizer efficiency effect.
Embodiment 4 utilizes embodiment 2 to produce the effect of bacillus wettable powder in flue-cured tobacco containing brassin lactones
1. test site: Bai Ju village, beachhead town, Longhui County, Hunan Province Cotton Varieties by Small Farming Households ground
2. test method: flue-cured tobacco cultivars: middle cigarette 99-10-1, establishes 2 process altogether, repeats for 4 times.Test area 16 mu, peripheral hardware protection row.Experimental group: during transplanted seedling, with bacillus wettable powder 1500 times of liquid irrigating roots once; Within after slow seedling 20 days, spray first time with bacillus wettable powder 1000 times of liquid, spray second time and third time every 20 days then continuously.Check plot is sprayed at each clear water with same consumption;
3. result of the test:
Because flue-cured tobacco is all planted in continuous 2 years in the soil in Bai Ju village, although also plant every year one season late rice, but its bacterial wilt, balck shank and the mosaic disease incidence of disease are higher, but after experimental group uses the bacillus wettable powder of brassinolide-containing, the plant height of flue-cured tobacco as described in Table 2, stem girth, pitch, leaves number, maximum leaf area are all greater than control group, illustrate that the bacillus wettable powder of brassinolide-containing effectively can improve the economical character of cigarette strain, promote cigarette strain robust growth.Cigarette strain bacterial wilt, balck shank and the mosaic disease incidence of disease used as shown in table 3 is very low, and occurring degree significantly reduces, control group then has obvious bacterial wilt, balck shank and mosaic disease, also obviously there is disease after usage chain mycin and 50% fenaminosulf wetting powder;
The bacillus wettable powder of table 2 brassinolide-containing in flue-cured tobacco to plant height, stem girth, the impact of leaf area
Table 3 uses the bacillus wettable powder of brassinolide-containing to the control efficiency of land for growing field crops tobacco to balck shank, bacterial wilt and mosaic disease
The bacillus wettable powder of table 4 brassinolide-containing is to tobacco production output value the effect of increasing income
| Process | Output (kg/acre) | The output value (unit/mu) | Average price (unit/kilogram) |
| Check plot | 204.4 | 2828.9 | 13.84 |
| Test region | 276.7 | 4222.4 | 15.26 |
Use tobacco premature ripening after the bacillus wettable powder of brassinolide-containing, improve quality, improve the output of tobacco leaf, average price, the output value, upper medium grade cigarette ratio to a great extent.Can analyze from table 4 and draw, est and pilot project (treatment region) yield of tobacco, the output value, average price exceed 72.3 kgs/acre, 1393.5 yuan/mu, 1.42 yuan/kilogram than control zone respectively.Deduct the cost 440 yuan (wherein artificial 360 yuan) of increase, 953.5 yuan/mu can be had a net increase of, significantly can increase profit.
Claims (6)
1. the bacillus wettable powder containing brassin lactones; it is characterized in that; each composition part of described wetting powder is by weight: brassin lactones 0.001-0.01 part; bacillus bacterium liquid 100-200 part; emulsifier: polyoxyethylene nonylphenol ether 0.01-0.1 part; solvent: ethanol 0.15-1.5 part; gemma protectant: skim milk powder 2-10 part; methylcellulose 1-5 part; dispersant: D4252-12 part; wetting agent: neopelex 4-20 part, carrier: diatomite 50-100 part forms.
2. the preparation method of a kind of bacillus wettable powder containing brassin lactones according to claim 1, is characterized in that, the bacillus wettable powder of preparation containing brassin lactones comprises following steps:
, bacillus bacterium liquid preparation
Take out culture presevation pipe, draw flat board with LB solid culture medium and recover, cultivate 48 hours for 37 DEG C; Under flat board in the single colony inoculation of picking 50 milliliters of LB medium, in incubator, 37 DEG C shake cultivation 24 hours; Seed adopts the inoculum concentration of 5%, and be seeded to the bacillus liquid medium in the large triangular flask of 5L, liquid amount is 2L, and 20-28 hour is cultivated in 37 DEG C of concussions, and detect its bacterial concentration, spore content, more than 8,000,000,000 CFU/mL, namely can be used as bacillus bacterium liquid; Wherein said LB medium: peptone 5g, beef extract 3g, sodium chloride 5g, water 1000mL, pH7.2, add agar 2% in solid culture medium; Wherein said bacillus liquid medium: glucose 10g/L, corn flour 18g/L, wheat bran 25g/L, fish meal 5g/L, magnesium sulfate 0.5g/L, manganese sulphate 0.5g/L, sodium dihydrogen phosphate 0.5g/L, sodium hydrogen phosphate 2.3g/L, pH7.2; The condition of each medium sterilization is: 0.10-0.15MPa, 121 DEG C of sterilizings 30 minutes;
, brassin lactones pretreatment
First brassin lactones 0.001-0.01 part is joined in ethanol 0.15-1.5 part and dissolves, then add polyoxyethylene nonylphenol ether 0.01-0.1 part to it, mixing brassin lactones solution;
, by step
the brassin lactones solution of preparation is added to step
in 100-200 part bacillus bacterium liquid of preparation, then add skim milk powder 2-10 part successively, methylcellulose 1-5 part, D4252-12 part, neopelex 4-20 part, diatomite 50-100 part, spraying dry after mixing, water content is lower than 5%, air-flow crushing crosses 400 mesh sieves, and detect not low 10,000,000,000 CFU/g of spore content, suspensibility is not less than 80%, wetting time, not higher than 70s, is the bacillus wettable powder containing brassin lactones.
3. a kind of bacillus wettable powder containing brassin lactones according to claim 1; it is characterized in that it respectively forms part and is by weight: brassin lactones 0.005 part; bacillus bacterium liquid 150 parts; emulsifier: polyoxyethylene nonylphenol ether 0.05 part, solvent: ethanol 0.75 part, gemma protectant: skim milk powder 5 parts; methylcellulose 2 parts; dispersant: D4253 part, wetting agent: neopelex 5 parts, carrier: 70 parts, diatomite composition.
4. a kind of bacillus wettable powder containing brassin lactones according to claim 1, it is characterized in that, described bacillus bacterium liquid is by bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens, Paenibacillus polymyxa, bacillus megaterium, bacillus cereus, bacillus laterosporus, bacillus marinus, bacillus firmus, atrophy bacillus, methyl type bacillus, waxy Bacillus, not Kazakhstan bacillus, Bacillus mucilaginosus, Bacillus cercus, bacillus pumilus, bafillus natto, one or more mixtures in bacillus coagulans.
5. a kind of bacillus wettable powder containing brassin lactones according to claim 1, it is characterized in that, the content of described brassin lactones is not less than 90%.
6. a kind of bacillus wettable powder containing brassin lactones according to claim 1, is characterized in that, described a kind of bacillus wettable powder containing brassin lactones in paddy rice, flue-cured tobacco, corn, wheat, strawberry, watermelon, capsicum, tomato, the application on the crops such as cucumber.
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| CN106465732A (en) * | 2016-08-31 | 2017-03-01 | 赵呈浩 | A kind of fruit surface protecting film |
| CN106879585A (en) * | 2017-03-10 | 2017-06-23 | 上海交通大学 | Trichoderma brassin lactones wettable powder and its preparation method and application |
| CN107581198A (en) * | 2017-08-25 | 2018-01-16 | 河南中麦生物科技有限公司 | It is a kind of extremely to be brought back to life bacterium solution and its application for vegetable root |
| CN108101625A (en) * | 2017-12-01 | 2018-06-01 | 芮志行 | A kind of preparation method for the fertilizer for being used to prevent rice blast |
| CN109805007A (en) * | 2017-11-22 | 2019-05-28 | 河南福瑞得生物科技有限公司 | A kind of bacillus subtilis wettable powder |
| CN112314626A (en) * | 2020-11-09 | 2021-02-05 | 青岛农业大学 | Preparation and application method of biological stimulator for improving activity of continuous cropping salvia miltiorrhiza seedlings |
| CN113087572A (en) * | 2021-05-18 | 2021-07-09 | 阎昭良 | Hydrogen-containing fulvic acid-brassinolide stress-resistant nutrient solution and preparation and application thereof |
| WO2021240315A1 (en) * | 2020-05-27 | 2021-12-02 | Setter Dylan John | Probiotic bacillus sanitiser |
| CN114903055A (en) * | 2021-12-30 | 2022-08-16 | 云南省微生物发酵工程研究中心有限公司 | Wettable powder for preventing and treating black spot disease of Chinese cabbage, preparation method and application |
| CN115349519A (en) * | 2022-08-30 | 2022-11-18 | 山东大成生物化工有限公司 | Brassinolide-gibberellic acid-bacillus aryabhattai compound pesticide fertilizer and preparation method and application thereof |
| RU2800257C1 (en) * | 2022-06-10 | 2023-07-19 | Андрей Анатольевич Амелин | Growth accelerator of the root system of plants (embodiments) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106465732A (en) * | 2016-08-31 | 2017-03-01 | 赵呈浩 | A kind of fruit surface protecting film |
| CN106879585A (en) * | 2017-03-10 | 2017-06-23 | 上海交通大学 | Trichoderma brassin lactones wettable powder and its preparation method and application |
| CN106879585B (en) * | 2017-03-10 | 2020-11-17 | 上海交通大学 | Trichoderma brassinolide wettable powder and preparation method and application thereof |
| CN107581198A (en) * | 2017-08-25 | 2018-01-16 | 河南中麦生物科技有限公司 | It is a kind of extremely to be brought back to life bacterium solution and its application for vegetable root |
| CN109805007A (en) * | 2017-11-22 | 2019-05-28 | 河南福瑞得生物科技有限公司 | A kind of bacillus subtilis wettable powder |
| CN108101625A (en) * | 2017-12-01 | 2018-06-01 | 芮志行 | A kind of preparation method for the fertilizer for being used to prevent rice blast |
| WO2021240315A1 (en) * | 2020-05-27 | 2021-12-02 | Setter Dylan John | Probiotic bacillus sanitiser |
| CN112314626A (en) * | 2020-11-09 | 2021-02-05 | 青岛农业大学 | Preparation and application method of biological stimulator for improving activity of continuous cropping salvia miltiorrhiza seedlings |
| CN112314626B (en) * | 2020-11-09 | 2022-04-01 | 青岛农业大学 | Application of biological stimulin for improving activity of continuous cropping salvia miltiorrhiza seedlings |
| CN113087572A (en) * | 2021-05-18 | 2021-07-09 | 阎昭良 | Hydrogen-containing fulvic acid-brassinolide stress-resistant nutrient solution and preparation and application thereof |
| CN114903055A (en) * | 2021-12-30 | 2022-08-16 | 云南省微生物发酵工程研究中心有限公司 | Wettable powder for preventing and treating black spot disease of Chinese cabbage, preparation method and application |
| CN114903055B (en) * | 2021-12-30 | 2024-03-08 | 云南省微生物发酵工程研究中心有限公司 | Wettable powder for preventing and treating black spot of celery cabbage, preparation method and application |
| RU2800257C1 (en) * | 2022-06-10 | 2023-07-19 | Андрей Анатольевич Амелин | Growth accelerator of the root system of plants (embodiments) |
| CN115349519A (en) * | 2022-08-30 | 2022-11-18 | 山东大成生物化工有限公司 | Brassinolide-gibberellic acid-bacillus aryabhattai compound pesticide fertilizer and preparation method and application thereof |
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