CN116358980B - Tumor cell short-term test identification device - Google Patents
Tumor cell short-term test identification device Download PDFInfo
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- CN116358980B CN116358980B CN202310636483.3A CN202310636483A CN116358980B CN 116358980 B CN116358980 B CN 116358980B CN 202310636483 A CN202310636483 A CN 202310636483A CN 116358980 B CN116358980 B CN 116358980B
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- 210000004881 tumor cell Anatomy 0.000 title claims abstract description 92
- 238000012031 short term test Methods 0.000 title claims description 4
- 238000000605 extraction Methods 0.000 claims abstract description 132
- 239000008280 blood Substances 0.000 claims abstract description 72
- 210000004369 blood Anatomy 0.000 claims abstract description 72
- 239000011324 bead Substances 0.000 claims abstract description 41
- 238000001514 detection method Methods 0.000 claims abstract description 39
- 239000011248 coating agent Substances 0.000 claims abstract description 12
- 238000000576 coating method Methods 0.000 claims abstract description 12
- 238000010828 elution Methods 0.000 claims description 56
- 239000007788 liquid Substances 0.000 claims description 44
- 239000007921 spray Substances 0.000 claims description 21
- 238000007789 sealing Methods 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 238000011161 development Methods 0.000 claims description 12
- 230000000903 blocking effect Effects 0.000 claims description 6
- 230000008878 coupling Effects 0.000 claims description 6
- 238000010168 coupling process Methods 0.000 claims description 6
- 238000005859 coupling reaction Methods 0.000 claims description 6
- 238000005086 pumping Methods 0.000 claims description 5
- 238000009434 installation Methods 0.000 claims description 4
- 238000001179 sorption measurement Methods 0.000 abstract description 17
- 206010028980 Neoplasm Diseases 0.000 description 9
- 230000000694 effects Effects 0.000 description 6
- 230000006835 compression Effects 0.000 description 5
- 238000007906 compression Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 230000004888 barrier function Effects 0.000 description 3
- 238000010009 beating Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 230000030944 contact inhibition Effects 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000013100 final test Methods 0.000 description 1
- 230000003116 impacting effect Effects 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000001617 migratory effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/405—Concentrating samples by adsorption or absorption
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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Abstract
The invention discloses a tumor cell rapid detection and identification device, which relates to the technical field of tumor cell rapid detection and identification. The invention discloses a tumor cell rapid detection and identification device which is provided with an antibody coating magnetic bead at the outer side of a preliminary extraction arc plate for preliminary extraction of tumor cells in an electrified state when tumor cells in blood are extracted, the extracted blood enters a lower space through perforation, when the blood passes through each extraction hole, the antibody coating magnetic bead in the extraction hole adsorbs the tumor cells, the adsorbed blood falls into the lower space, and meanwhile, a driving motor I is started to drive a built-in extraction tube to rotate, so that the adsorption value of the antibody coating magnetic bead in each extraction hole can be realized, the extraction rate of tumor cells in the same batch of blood is improved, and the accuracy of a final detection result is improved.
Description
Technical Field
The invention relates to the technical field of tumor cell rapid detection and identification, in particular to a tumor cell rapid detection and identification device.
Background
The tumor cell parenchyma is a tumor. The tumor tissue consists of a parenchyma part and a stroma part, and the tumor parenchyma is tumor cells, is the main component of the tumor, and has tissue origin specificity. It determines the biological characteristics of the tumor and the specificity of each tumor. The tissue sources of various tumors are generally identified according to the substantial morphology of the tumor, classification, naming and histological diagnosis of the tumor are performed, and benign and malignant tumors and malignant tumor degrees are determined according to the differentiation maturity and the atypical size thereof. Tumor cells have three significant basic features: dead, migratory and loss of contact inhibition. In addition, tumor cells have many physiological, biochemical and morphological characteristics that differ from those of normal cells; in the treatment of patients, it is necessary to detect and identify the cells in their blood by means of a rapid detection and identification component.
When the existing tumor cell rapid detection and identification device is used, tumor cells are generally adsorbed through an adsorption rod coated with antibody coated magnetic beads, however, after blood passes through the adsorption rod, the antibody coated magnetic beads in an electrified state are coated with tumor cells after adsorbing the tumor cells, and when the blood passes through again, the antibody coated magnetic beads cannot continuously adsorb the tumor cells, so that the quantitative detection result of the content of the tumor cells in the blood deviates, and the use value of the tumor cell rapid detection and identification device is reduced.
As disclosed in chinese patent No. CN202210616326.1, a single-sampling and automatic identifying machine for multiple kinds of circulating tumor cells is disclosed, wherein a circulating pump is disposed on a side surface of an identifying body, a separation filter cavity is disposed in the identifying body, a movable lifting assembly is disposed in the identifying body, a magnetic control chuck extending into the separation filter cavity is disposed at an output end of the movable assembly, and an adsorption rod is fixedly mounted in the magnetic control chuck. This multiple circulation tumor cell singly adopts and automatic identification machine, dock two pipes with patient's blood vessel, then through the operation of circulating pump, the blood of extraction enters into the inside of separation filter chamber, the inside of separation filter chamber is provided with the adsorption pole, the surface of adsorption pole is adsorbed uniformly has antibody coated magnetic bead, antibody coated magnetic bead can combine together with the marker on tumor cell surface to adsorb the tumor cell at the surface of adsorption pole, reach the effect of filtering or screening tumor cell in the blood. However, after the antibody coated magnetic beads of the identification device adsorb tumor cells, the surfaces of the antibody coated magnetic beads are coated by the tumor cells, and when blood passes through again, the antibody coated magnetic beads cannot continuously adsorb the tumor cells, so that the screening and identification errors are large, and the identification speed is low.
Therefore, the problem of slow identification speed caused by the identification error due to the fact that tumor cells in all blood samples cannot be continuously detected and identified is solved.
Disclosure of Invention
The invention discloses a tumor cell rapid detection and identification device, which aims to solve the technical problems that when the traditional tumor cell rapid detection and identification device is used, tumor cells are generally adsorbed through an adsorption rod coated with antibody-coated magnetic beads, however, after blood passes through the adsorption rod, the antibody-coated magnetic beads in an electrified state are coated with tumor cells after adsorbing the tumor cells, and when the blood passes through again, the antibody-coated magnetic beads cannot continuously adsorb the tumor cells, so that the quantitative detection result of the content of the tumor cells in the blood is deviated.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the utility model provides a tumour cell short-term test identification device, includes base and extraction component, its characterized in that, extraction component includes water conservancy diversion case and sealing frame, and open the bottom of water conservancy diversion case has the mounting hole, and the inside fixedly connected with trapezoidal reposition of redundant personnel frame of mounting hole, and the top equidistance of trapezoidal reposition of redundant personnel frame leaves there is the inclined water conservancy diversion hole that runs through, and sealing frame is laminated with the bottom of trapezoidal reposition of redundant personnel frame mutually, the inboard fixedly connected with preliminary extraction arc board that is close to the top of sealing frame, and the outside equidistance of preliminary extraction arc board leaves there is the perforation, one side fixedly connected with driving motor one, and driving motor one's output shaft passes through shaft coupling fixedly connected with built-in extraction tube, and the other end of built-in extraction tube passes through the bearing to be connected to sealing frame's inner wall, and the outside equidistance of built-in extraction tube leaves there is the extraction hole, and the both sides that preliminary extraction arc board is close to the bottom are fixedly connected with barrier plate, and contact between barrier plate and the built-in extraction tube, the outside of preliminary extraction arc board and the inboard of extraction hole all are equipped with antibody coating magnetic bead;
the outside of the diversion box close to the lower part is fixedly connected with a mounting rod, the other end of the mounting rod is fixedly connected with a forward and backward rotating motor, an output shaft of the forward and backward rotating motor is rotationally connected with a rotating shaft, the bottom of the rotating shaft is fixedly connected with a rotating frame, the outside of the rotating frame is fixedly connected with a collecting frame, the collecting frame is used for storing a blood sample to be detected, the collecting frame is located the below of built-in extraction pipe, set up first drawing liquid pump on the rotating turret, the feed liquor pipe and the collecting frame bottom intercommunication of first drawing liquid pump, the drain pipe is connected to the liquid outlet of first drawing liquid pump, the shower nozzle in a inboard of water conservancy diversion case is fixed in the other end connection of drain pipe, just the spout direction of shower nozzle is aimed at the inboard that water conservancy diversion case and shower nozzle subtended.
Through being provided with extraction element, when carrying out the tumour cell in the blood and draw, in leading-in baffle box of this part blood, make it evenly scatter to preliminary extraction arc board through each put the water conservancy diversion hole to one side, preliminary extraction arc board outside antibody coating magnetic bead carries out preliminary extraction to tumour cell under the switch-on state, the blood after the extraction passes through the perforation and gets into the space of below, the space below forms a class enclosure space under the partial position effect of two barrier plates, preliminary extraction arc board and built-in extraction tube, when blood passes in each extraction hole, the antibody coating magnetic bead in the extraction hole adsorbs tumour cell, the blood after the absorption falls into the space of below, simultaneously, drive motor one drives built-in extraction tube and rotates, thereby make the inside antibody coating magnetic bead of each extraction hole all can realize its adsorption value, improve the tumour cell extraction rate in the same batch blood, thereby improve the accuracy of final testing result.
In a preferred scheme, the inside of built-in extraction tube is fixedly connected with interior pole, and the outside equidistance of interior pole distributes has elution auxiliary assembly, and elution auxiliary assembly includes the collar.
In a preferred scheme, the collar fixed connection is in the outside of interior pole, and one side fixedly connected with two pneumatic cylinders two of collar, the same clamping ring of output fixedly connected with of two pneumatic cylinders two, and the outside annular distribution that the collar is close to the central point has the elastic rod, and the other end of every elastic rod all is equipped with and strikes the ball, and the clamping ring is located the outside of a plurality of elastic rods.
Through being provided with elution auxiliary assembly, when extracting the tumor cell elution operation on the subassembly, adjust pneumatic cylinder two and drive the clamping ring and remove towards the position that is close to the collar for the clamping ring can't prescribe a limit to the elastic rod, and driving motor one drives built-in extraction tube and rotates, and the elastic rod is tensile under rotatory drive, thereby drives to strike the ball and beats the inboard of built-in extraction tube, accelerates that tumor cell drops from antibody coating magnetic bead, improves elution efficiency, improves elution effect.
In a preferred scheme, the top fixedly connected with cylinder of base, and the output fixedly connected with lifter of cylinder, lifter fixedly connected with is in the outside of sealing frame, and one side fixedly connected with erection column of base, the other end fixedly connected with of erection column is in the outside of water conservancy diversion case.
In a preferred scheme, the outside that the water conservancy diversion case is close to the below fixedly connected with installation pole, and the other end fixedly connected with positive and negative rotation motor of installation pole, positive and negative rotation motor's output shaft is connected with the axis of rotation through the shaft coupling.
In a preferred scheme, the bottom fixedly connected with rotating frame of axis of rotation, and the outside fixedly connected with of rotating frame collects the frame, collects the frame and is located the below of built-in extraction tube.
The top fixedly connected with elution pond of base, and elution pond is located the below of collecting the frame, one side fixedly connected with pump frame in elution pond, the top fixedly connected with elution pump of pump frame, the conveyer pipe of conveyer pipe fixedly connected with of elution pump, the top fixedly connected with cavity spouts the board that the elution pond is close to the elution pump, the other end of conveyer pipe peg graft in the inside of cavity spouts the board, and the outside equidistance that cavity spouts the board and leave to elution pond inside has the orifice.
In a preferred scheme, the first liquid pumping pump quantitatively pumps a part of blood samples and then closes the liquid pumping pump, the pumped blood is sprayed out through the spray head, the blood sprayed out by the spray head falls to the trapezoid flow distribution frame to enter the inclined flow guide hole after impacting the inner wall of the flow guide box, meanwhile, the first liquid pumping pump is started to drive the built-in extraction pipe to rotate, after the preset time is reached, the positive and negative rotation motor is started to drive the rotating frame and the collection frame to rotate 90 degrees to the left side at the same time, at the moment, the air cylinder is started to drive the lifting rod to move downwards, the extraction pipe is moved into the elution pool to perform elution, after the elution is finished, the air cylinder is started to drive the lifting rod to move upwards, the extraction pipe is moved out, then the first liquid pumping pump is started again, the blood in the collection frame is quantitatively extracted into the flow guide box, and the continuous detection is realized.
In a preferred scheme, the outside of elution pond is equipped with even chromogenic assembly, and even chromogenic assembly includes color development circle frame and movable rail, and movable rail fixed connection is in one side of elution pond.
In a preferred scheme, one end of the movable rail is fixedly connected with a first hydraulic cylinder, the output end of the first hydraulic cylinder is fixedly connected with a sliding block, the sliding block is slidably connected to the inside of the movable rail, the top of the sliding block is fixedly connected with a second driving motor, the output shaft of the second driving motor is fixedly connected with a driving shaft through a coupling, and the color display ring frame is fixedly connected to the top of the driving shaft.
In a preferred scheme, the top fixedly connected with second drawing liquid pump of elution pond, the elution pond is located the one side fixedly connected with dead lever of removal rail top, and the outside fixedly connected with burette of dead lever, burette are located the color ring frame directly over, and the extraction end fixedly connected with extraction pipe of second drawing liquid pump, and the extraction pipe is located the inside of elution pond, and the delivery end of second drawing liquid pump passes through the inside of pipe connection in the burette.
Through being provided with even chromogenic subassembly, when the tumour cell after will eluting is leading-in the chromogenic ring frame, start the second drawing liquid pump, the second drawing liquid pump is carrying out tumour cell extraction in-process, and driving motor two drives the chromogenic ring frame and rotates, simultaneously, and pneumatic cylinder one is driven the chromogenic ring frame and is removed for finally through the tumour cell of burette drip onto the chromogenic ring frame evenly spread on the chromogenic liquid, the staff can observe the chromogenic position fast, improves the chromogenic effect.
The invention provides a tumor cell rapid detection and identification device, which quantitatively extracts a part of blood sample through a first liquid extracting pump, then closes the device, the extracted blood is sprayed out through a spray head, the blood sprayed out by the spray head impacts the inner wall of a diversion box and then falls to a trapezoid diversion frame to enter an inclined diversion hole, meanwhile, a driving motor is started to drive a built-in extraction pipe to rotate, after reaching a preset time, a positive and negative rotating motor is started to drive the rotation frame and a collection frame to rotate 90 degrees to the left side at the same time, at the moment, a cylinder is started to drive a lifting rod to move downwards, the extraction pipe is moved into an elution pool for elution, after the elution is finished, the cylinder is started to drive the lifting rod to move upwards, the extraction pipe is moved out, then the first liquid extracting pump is started again, and the blood in the collection frame is quantitatively extracted into the diversion box, thus circulation is realized, and when the tumor cell in the blood is extracted, the part of blood is led into a baffle box, so that the blood is uniformly scattered to a preliminary extraction arc plate through each inclined baffle hole, antibody coated magnetic beads at the outer side of the preliminary extraction arc plate are used for preliminary extraction of tumor cells in an electrified state, the extracted blood enters a lower space through perforation, the lower space forms a similar closed space under the action of the two baffle plates, the preliminary extraction arc plates and part of the positions of the built-in extraction tubes, when the blood passes through each extraction hole, the antibody coated magnetic beads in the extraction holes adsorb the tumor cells, the adsorbed blood falls into the lower space, meanwhile, a driving motor drives the built-in extraction tubes to rotate, so that the antibody coated magnetic beads in each extraction hole can realize the adsorption value, the extraction rate of tumor cells in the same batch of blood is improved, therefore, the technical effect of the accuracy of the final detection result is improved, and the detection and identification are continuous due to the quantitative extraction and the cyclic detection of the blood sample, so that the detection accuracy is higher.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a tumor cell rapid detection and identification device according to the present invention;
FIG. 2 is a side view showing the overall structure of a device for rapidly detecting and identifying tumor cells according to the present invention;
FIG. 3 is a schematic diagram of the internal structure of an extraction assembly of a device for rapid detection and identification of tumor cells according to the present invention;
FIG. 4 is a partial cross-sectional view of the structure of FIG. 3;
FIG. 5 is a cross-sectional view of a built-in extraction tube of a tumor cell rapid detection and identification device according to the present invention;
FIG. 6 is a schematic diagram of an elution accessory assembly of a tumor cell rapid detection and identification device according to the present invention;
fig. 7 is a schematic diagram of a uniform color development assembly of a device for rapidly detecting and identifying tumor cells according to the present invention.
In the figure: 1. a base; 2. a rotating frame; 3. an elution pool; 4. a rotating shaft; 5. a forward and reverse rotation motor; 6. a mounting rod; 7. a collection frame; 701. a first liquid pump; 702. a liquid outlet pipe; 703. a spray head; 8. an extraction assembly; 801. a diversion box; 802. a sealing frame; 803. driving a first motor; 804. a trapezoidal shunt frame; 805. a deflector hole is obliquely arranged; 806. initially extracting an arc plate; 807. perforating; 808. a blocking plate; 809. an extraction tube is arranged in the device; 810. extracting holes; 9. a mounting column; 10. a lifting rod; 11. a cylinder; 12. a uniform color development assembly; 1201. color ring display frame; 1202. a moving rail; 1203. a second driving motor; 1204. a drive shaft; 1205. a sliding block; 1206. a first hydraulic cylinder; 1207. a dropper; 1208. a fixed rod; 1209. a second liquid pump; 1210. an extraction tube; 13. a pump frame; 14. an elution pump; 15. a delivery tube; 16. a hollow spray plate; 17. an inner rod; 18. an elution assist assembly; 1801. a mounting ring; 1802. a second hydraulic cylinder; 1803. a compression ring; 1804. striking a ball; 1805. an elastic rod.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments.
The invention discloses a tumor cell rapid detection and identification device which is mainly applied to the existing tumor cell rapid detection and identification device, when the tumor cell rapid detection and identification device is used, the adsorption of tumor cells is generally carried out through an adsorption rod coated with antibody coated magnetic beads, however, after blood passes through the adsorption rod, the antibody coated magnetic beads in an electrified state are coated with tumor cells after the adsorption of tumor cells, and when the blood passes through again, the adsorption of the tumor cells cannot be continuously completed by the antibody coated magnetic beads, so that the quantitative scene that the detection result of the content of the tumor cells in the blood deviates is caused.
Referring to fig. 1-7, a tumor cell rapid detection and identification device comprises a base 1 and an extraction assembly 8, the extraction assembly 8 comprises a flow guide box 801 and a sealing frame 802, a mounting hole is formed in the bottom of the flow guide box 801, a trapezoid flow distribution frame 804 is fixedly connected to the outer side of the mounting hole, a penetrating inclined flow guide hole 805 is formed in the top of the trapezoid flow distribution frame 804 in an equidistant manner, the sealing frame 802 is attached to the bottom of the trapezoid flow distribution frame 804, a primary extraction arc plate 806 is fixedly connected to the inner side of the sealing frame 802 close to the top, through holes 807 are formed in the outer side of the primary extraction arc plate 806 in an equidistant manner, a driving motor 803 is fixedly connected to one side of the sealing frame 802, an output shaft of the driving motor 803 is fixedly connected with a built-in extraction pipe 809 through a coupler, the other end of the built-in extraction pipe 809 is connected to the inner wall of the sealing frame 802 through a bearing, an extraction hole 810 is formed in the outer side of the built-in extraction pipe 809 in an equidistant manner, a blocking plate 808 is fixedly connected to the two sides of the primary extraction arc plate 806 close to the bottom, the blocking plate 808 is in contact with the built-in extraction pipe 809, and antibody coated magnetic beads are arranged on the outer side of the primary extraction arc plate 806 and the inner side of the extraction hole 810.
In a specific application scenario, when tumor cells in blood are extracted, the partial blood is led into the diversion box 801, so that the partial blood is uniformly scattered to the preliminary extraction arc plate 806 through each inclined diversion hole 805, the antibody coated magnetic beads outside the preliminary extraction arc plate 806 are used for preliminary extraction of the tumor cells in an electrified state, the extracted blood enters a space below through the perforation 807, a similar closed space is formed in the space below under the action of the two blocking plates 808, the preliminary extraction arc plate 806 and part of the built-in extraction tube 809, when the blood passes through each extraction hole 810, as the magnetic beads coated with the antibodies can be combined with the markers on the surfaces of the tumor cells, the tumor cells are adsorbed on the surfaces of the extraction holes 810, the adsorbed blood falls into the space below by the antibody coated magnetic beads in the extraction holes 810, and meanwhile, the driving motor is started to drive the built-in extraction tube 809 to rotate, so that the antibody coated magnetic beads in each extraction hole 810 can realize the adsorption value, the final detection result of the tumor cells 803 in the same batch can be improved, and the accuracy of the final detection result of the tumor cells 803 in the same batch can be improved.
Referring to fig. 1, 5 and 6, in a preferred embodiment, an inner rod 17 is fixedly connected to the inside of the built-in extraction tube 809, elution auxiliary assemblies 18 are equidistantly distributed on the outer side of the inner rod 17, each elution auxiliary assembly 18 comprises a mounting ring 1801, the mounting rings 1801 are fixedly connected to the outer side of the inner rod 17, two hydraulic cylinders 1802 are fixedly connected to one side of each mounting ring 1801, the output ends of the two hydraulic cylinders 1802 are fixedly connected to the same pressing ring 1803, elastic rods 1805 are annularly distributed on the outer side, close to the center point, of each mounting ring 1801, a beating ball 1804 is arranged on the other end of each elastic rod 1805, and the pressing rings 1803 are located on the outer sides of the plurality of elastic rods 1805.
Specifically, when the tumor cell on the extraction assembly 8 is eluted, the second hydraulic cylinder 1802 is adjusted to drive the compression ring 1803 to reciprocate, so that the compression ring 1803 cannot limit the elastic rod 1805, the first driving motor 803 drives the built-in extraction tube 809 to rotate, the elastic rod 1805 stretches under the driving of rotation, and accordingly the beating ball 1804 is driven to beat the inner side of the built-in extraction tube 809, the tumor cells are accelerated to fall off from the antibody coated magnetic beads, the eluting efficiency is improved, and meanwhile the eluting effect is improved.
Referring to fig. 1 and 2, in a preferred embodiment, a cylinder 11 is fixedly connected to the top of a base 1, a lifting rod 10 is fixedly connected to the output end of the cylinder 11, the lifting rod 10 is fixedly connected to the outer side of a sealing frame 802, one side of the base 1 is fixedly connected to a mounting column 9, the other end of the mounting column 9 is fixedly connected to the outer side of a diversion box 801, the outer side of the diversion box 801 close to the lower side is fixedly connected to a mounting rod 6, the other end of the mounting rod 6 is fixedly connected to a forward and reverse rotation motor 5, an output shaft of the forward and reverse rotation motor 5 is rotatably connected to a rotation shaft 4, the bottom of the rotation shaft 4 is fixedly connected to a rotating frame 2, the outer side of the rotating frame 2 is fixedly connected to a collection frame 7, the collection frame 7 is used for storing a blood sample to be detected, the collection frame 7 is located below a built-in extraction tube 809, a first liquid pump 701 is arranged on the rotating frame 2, the feed liquor pipe and the collection frame 7 bottom intercommunication of first drawing liquid pump 701, the drain pipe 702 is connected to the liquid outlet of first drawing liquid pump 701, the shower nozzle 703 of fixing in a water conservancy diversion case 801 inboard is connected to the other end of drain pipe 702, and the spout direction of shower nozzle 703 is aimed at the inboard that water conservancy diversion case 801 and shower nozzle 703 are subtended, collection frame 7 is located the below of built-in extraction pipe 809, the top fixedly connected with elution pond 3 of base 1, and elution pond 3 is located the below of collection frame 7, one side fixedly connected with pump frame 13 of elution pond 3, the top fixedly connected with elution pump 14 of pump frame 13, the conveyer pipe 15 of elution pump 14 is fixed connection, the top fixedly connected with cavity spray plate 16 that elution pond 3 is close to elution pump 14, the other end of conveyer pipe 15 is pegged graft in the inside of cavity spray plate 16, the outside equidistance that cavity spray plate 16 is inside towards elution pond 3 leaves there is the orifice.
In the actual detection and authentication, the continuous detection and authentication of tumor cells must be realized, and the accuracy of detection must be maintained, and specifically, the invention is that after a part of blood sample is quantitatively extracted by the first liquid extracting pump 701, the extracted blood is closed and sprayed out by the spray nozzle 703, the blood sprayed out by the spray nozzle 703 hits the inner wall of the flow guiding box 801 and falls to the trapezoid flow dividing frame 804 to enter the inclined flow guiding hole 805, meanwhile, the first driving motor 803 is started to drive the built-in extraction tube 809 to rotate, after reaching a preset time, the forward and reverse rotating motor 5 is started to drive the rotating frame 2 and the collecting frame 7 to rotate 90 degrees to the left side at the same time, at this time, the cylinder 11 is started to drive the lifting rod 10 to move downwards, the extraction tube 1210 is moved into the eluting tank 3 for eluting, after eluting is finished, the cylinder 11 is started to drive the lifting rod 10 to move upwards, the extraction tube 1210 is removed, then the first liquid extracting pump 701 is started again, and the blood in the collecting frame 7 is quantitatively extracted into the flow guiding box 801, and thus circulated, and continuous detection is realized.
Referring to fig. 1, 2 and 7, in a preferred embodiment, the outer side of the eluting tank 3 is provided with a uniform color developing assembly 12, and the uniform color developing assembly 12 includes a color developing ring frame 1201 and a moving rail 1202, the moving rail 1202 is fixedly connected to one side of the eluting tank 3, one end of the moving rail 1202 is fixedly connected with a first hydraulic cylinder 1206, an output end of the first hydraulic cylinder 1206 is fixedly connected with a sliding block 1205, the sliding block 1205 is slidably connected to the inside of the moving rail 1202, a top of the sliding block 1205 is fixedly connected with a second driving motor 1203, an output shaft of the second driving motor 1203 is fixedly connected with a driving shaft 1204 through a coupling, the color developing ring frame 1201 is fixedly connected to the top of the driving shaft 1204, the top of the eluting tank 3 is fixedly connected with a second liquid extracting pump 1209, the eluting tank 3 is positioned above the moving rail 1202, a fixed rod 1208 is fixedly connected to the outer side of the fixed rod, the dropper 1207 is positioned right above the color developing ring frame 1201, an extracting pipe 1210 is fixedly connected to an extracting end of the second liquid extracting pump 1209, the extracting pipe 1210 is positioned inside the eluting tank 3, and the second liquid extracting pipe 1209 is connected to the inside of the dropper 1207 through a conveying pipe 1207.
When the tumor cells in the blood are extracted, the part of blood is guided into a collecting frame (7), then, a first liquid extracting pump 701 quantitatively extracts a part of blood sample and then is closed, the extracted blood is ejected through a nozzle 703, the blood ejected by the nozzle 703 impacts the inner wall of a flow guiding box 801 and then falls onto a trapezoid flow dividing frame 804 to enter inclined flow guiding holes 805, so that the blood is uniformly poured into an initial extraction arc plate 806 through each inclined flow guiding hole 805, the antibody coated magnetic beads outside the initial extraction arc plate 806 perform initial extraction on the tumor cells under the electrified state, the extracted blood enters a lower space through a perforation 807, the lower space forms a similar closed space under the action of the two blocking plates 808, the initial extraction arc plate 806 and part of the built-in extraction tube 809, when the blood passes through each extraction hole 810, the antibody coated magnetic beads in the extraction holes 810 adsorb the tumor cells, the absorbed blood falls into the space below, meanwhile, the driving motor I803 is started, the driving motor I803 drives the built-in extraction tube 809 to rotate, so that the antibody coating magnetic beads in each extraction hole 810 can realize the absorption value, after tumor cells are extracted, the adjusting cylinder 11 drives the built-in extraction tube 809 and the preliminary extraction arc plate 806 to enter the eluting tank 3, the eluting pump 14 is started, the eluting pump 14 drives the eluent to elute the tumor cells, in the eluting process, the adjusting cylinder II 1802 drives the compression ring 1803 to reciprocate, the compression ring 1803 cannot limit the elastic rod 1805, the driving motor I803 drives the built-in extraction tube 809 to rotate, the elastic rod 1805 stretches under the rotation driving, the beating ball 1804 is driven to beat the inner side of the built-in extraction tube 809, the tumor cells are accelerated to fall off from the antibody coating magnetic beads, after elution is completed, the second liquid pump 1209 is started, the second liquid pump 1209 drives the color development ring frame 1201 to rotate by the driving motor II 1203 in the process of extracting tumor cells, and meanwhile, the first hydraulic cylinder 1206 drives the color development ring frame 1201 to move, so that the tumor cells which are finally dripped onto the color development ring frame 1201 through the drip tube 1207 are uniformly spread on the color development liquid, and workers can quickly observe the color development part to obtain a final identification result.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.
Claims (7)
1. The utility model provides a tumor cell short-term test appraisal device, includes base (1) and extraction component (8), its characterized in that, extraction component (8) include water conservancy diversion case (801) and sealing frame (802), and open the bottom of water conservancy diversion case (801) has the mounting hole, the inside fixedly connected with trapezoidal reposition of redundant personnel frame (804) of mounting hole, the top equidistance of trapezoidal reposition of redundant personnel frame (804) leaves there is oblique water conservancy diversion hole (805) that run through, sealing frame (802) laminate with the bottom of trapezoidal reposition of redundant personnel frame (804), the inboard fixedly connected with preliminary extraction arc board (806) near the top of sealing frame (802), and preliminary extraction arc board (806) outside equidistance leaves has perforation (807), one side fixedly connected with driving motor (803), and driving motor one (803)'s output shaft is through coupling fixedly connected with built-in extraction pipe (809), the other end of built-in extraction pipe (809) is connected with the inner wall of sealing frame (802) through the bearing, built-in extraction pipe (809) outside equidistance leaves there is extraction hole (810), preliminary extraction arc board (806) is close to both sides of blocking board (808) and contact between the equal fixedly connected with extraction arc board (808) and the contact between the two sides (808), antibody coated magnetic beads are arranged on the outer side of the preliminary extraction arc plate (806) and the inner side of the extraction hole (810);
the top of the base (1) is fixedly connected with an air cylinder (11), the output end of the air cylinder (11) is fixedly connected with a lifting rod (10), the lifting rod (10) is fixedly connected to the outer side of the sealing frame (802), one side of the base (1) is fixedly connected with a mounting column (9), and the other end of the mounting column (9) is fixedly connected to the outer side of the flow guide box (801);
the device is characterized in that an installation rod (6) is fixedly connected to the outer side of the diversion box (801) close to the lower side, a positive and negative rotating motor (5) is fixedly connected to the other end of the installation rod (6), a rotating shaft (4) is rotatably connected to an output shaft of the positive and negative rotating motor (5), a rotating frame (2) is fixedly connected to the bottom of the rotating shaft (4), a collecting frame (7) is fixedly connected to the outer side of the rotating frame (2), the collecting frame (7) is used for storing blood samples to be detected, the collecting frame (7) is located below a built-in extraction pipe (809), a first liquid suction pump (701) is arranged on the rotating frame (2), a liquid inlet pipe of the first liquid suction pump (701) is communicated with the bottom of the collecting frame (7), a liquid outlet of the first liquid suction pump (701) is connected with a liquid outlet pipe (702), the other end of the liquid outlet pipe (702) is connected with a spray head (703) fixed to the inner side of the diversion box (801), and the direction of a spray nozzle of the spray head (703) is aligned with the inner side of the diversion box (801) opposite to the spray head (703);
the first liquid pumping pump (701) quantitatively pumps a part of blood sample and then closes the blood after the blood sample is pumped, the pumped blood is sprayed out through the spray head (703), the blood sprayed out by the spray head (703) impacts the inner wall of the diversion box (801) and then falls into the trapezoid diversion frame (804) to enter the inclined diversion holes (805), the blood uniformly spills into the space below the inclined diversion holes (805) through the inclined diversion holes, the antibody coating magnetic beads outside the inclined diversion holes (806) are initially extracted in the electrified state, the extracted blood enters the space below the through the perforations (807), the space below the two baffle plates (808), the initial extraction arc plates (806) and the built-in extraction tubes (809) form a similar closed space, when the blood passes through the extraction holes (810), the antibody coating magnetic beads in the extraction holes (810) adsorb the tumor cells, meanwhile, a driving motor (803) is started, the motor (803) is driven to rotate, the extraction tubes (809) are driven by the driving motor (803) to rotate, the magnetic beads (803) are driven by the motor 803) to rotate, and the preset values are achieved, and the magnetic beads (803) are synchronously driven by the magnetic beads (803) to rotate, the preset values, the rotary frame (2) and the collecting frame (7) are driven to rotate 90 degrees to the left side simultaneously, at this time, the air cylinder (11) is started to drive the lifting rod (10) to move downwards, the extracting pipe (1210) is moved into the eluting tank (3) to elute, after the elution is finished, the air cylinder (11) is started to drive the lifting rod (10) to move upwards, the extracting pipe (1210) is moved out, then the first liquid extracting pump (701) is started again, and blood in the collecting frame (7) is quantitatively extracted to the inside of the flow guide box (801), and the circulation is realized to realize continuous detection.
2. The device for rapid detection and identification of tumor cells according to claim 1, wherein the inner part of the built-in extraction tube (809) is fixedly connected with an inner rod (17), and elution auxiliary components (18) are equidistantly distributed on the outer side of the inner rod (17), and the elution auxiliary components (18) comprise mounting rings (1801).
3. The device for rapidly detecting and identifying tumor cells according to claim 2, wherein the mounting ring (1801) is fixedly connected to the outer side of the inner rod (17), one side of the mounting ring (1801) is fixedly connected with two hydraulic cylinders (1802), the output ends of the two hydraulic cylinders (1802) are fixedly connected with the same pressing ring (1803), elastic rods (1805) are annularly distributed on the outer side, close to the central point, of the mounting ring (1801), the other end of each elastic rod (1805) is provided with a striking ball (1804), and the pressing ring (1803) is located on the outer side of the plurality of elastic rods (1805).
4. The device for rapidly detecting and identifying tumor cells according to claim 3, wherein the top of the base (1) is fixedly connected with an elution pool (3), the elution pool (3) is located below the collection frame (7), one side of the elution pool (3) is fixedly connected with a pump frame (13), the top of the pump frame (13) is fixedly connected with an elution pump (14), the conveying end of the elution pump (14) is fixedly connected with a conveying pipe (15), the elution pool (3) is close to the top of the elution pump (14) and is fixedly connected with a hollow spray plate (16), the other end of the conveying pipe (15) is inserted into the hollow spray plate (16), and the hollow spray plate (16) is provided with spray holes facing the outer side of the interior of the elution pool (3) and equidistantly separated.
5. The rapid tumor cell detection and identification device according to claim 4, wherein a uniform color development component (12) is arranged on the outer side of the elution pool (3), the uniform color development component (12) comprises a color development ring frame (1201) and a moving rail (1202), and the moving rail (1202) is fixedly connected to one side of the elution pool (3).
6. The device for rapid detection and identification of tumor cells according to claim 5, wherein one end of the moving rail (1202) is fixedly connected with a first hydraulic cylinder (1206), an output end of the first hydraulic cylinder (1206) is fixedly connected with a sliding block (1205), the sliding block (1205) is slidably connected to the inside of the moving rail (1202), a second driving motor (1203) is fixedly connected to the top of the sliding block (1205), an output shaft of the second driving motor (1203) is fixedly connected with a driving shaft (1204) through a coupling, and the color development ring frame (1201) is fixedly connected to the top of the driving shaft (1204).
7. The rapid tumor cell detection and identification device according to claim 6, wherein a second liquid extraction pump (1209) is fixedly connected to the top of the elution pool (3), a fixing rod (1208) is fixedly connected to one side of the elution pool (3) above the moving rail (1202), a dropper (1207) is fixedly connected to the outer side of the fixing rod (1208), the dropper (1207) is located right above the color-developing ring frame (1201), an extraction pipe (1210) is fixedly connected to the extraction end of the second liquid extraction pump (1209), the extraction pipe (1210) is located inside the elution pool (3), and the conveying end of the second liquid extraction pump (1209) is connected to the inside of the dropper (1207) through a pipeline.
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