CN114813302B - Multi-circulation tumor cell single sampling and automatic identifying machine - Google Patents
Multi-circulation tumor cell single sampling and automatic identifying machine Download PDFInfo
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- CN114813302B CN114813302B CN202210616326.1A CN202210616326A CN114813302B CN 114813302 B CN114813302 B CN 114813302B CN 202210616326 A CN202210616326 A CN 202210616326A CN 114813302 B CN114813302 B CN 114813302B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/405—Concentrating samples by adsorption or absorption
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150015—Source of blood
- A61B5/15003—Source of blood for venous or arterial blood
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150755—Blood sample preparation for further analysis, e.g. by separating blood components or by mixing
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150992—Blood sampling from a fluid line external to a patient, such as a catheter line, combined with an infusion line; blood sampling from indwelling needle sets, e.g. sealable ports, luer couplings, valves
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/153—Devices specially adapted for taking samples of venous or arterial blood, e.g. with syringes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/155—Devices specially adapted for continuous or multiple sampling, e.g. at predetermined intervals
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention relates to a single picking and automatic identifying machine for multiple circulating tumor cells, which comprises an identifying machine body, wherein a circulating pump is arranged on the side surface of the identifying machine body, a separation filter cavity is arranged in the identifying machine body, a movable lifting assembly is arranged in the identifying machine body, a magnetic control chuck extending to the inside of the separation filter cavity is arranged at the output end of the movable lifting assembly, and an adsorption rod is fixedly arranged in the magnetic control chuck. This multiple circulation tumor cell singly adopts and automatic identification machine, dock two pipes with patient's blood vessel, then through the operation of circulating pump, the blood of extraction enters into the inside of separation filter chamber, the inside of separation filter chamber is provided with the adsorption pole, the even absorption of surface of adsorption pole has antibody to coat the magnetic bead, the magnetic bead that the antibody was coated can combine together with the marker on tumor cell surface to adsorb the tumor cell at the surface of adsorption pole, reach the effect of filtering or screening tumor cell in the blood.
Description
Technical Field
The invention relates to the technical field of tumor cell identification equipment, in particular to a single sampling and automatic identification machine for multiple circulating tumor cells.
Background
Circulating tumor cells are tumor cells derived from primary tumors or metastatic tumors, gaining the ability to break away from the basement membrane and invade into blood vessels through the tissue stroma. Circulating tumor cells are increasingly becoming a research hotspot of clinical liquid biopsy markers and are increasingly widely used in clinic. The circulating tumor cells are present in the peripheral blood in very small amounts, possibly only 1 or a few per 10ml of blood, but contain about 1 hundred million white blood cells and about 500 hundred million red blood cells at the same time, and efficient separation of the circulating tumor cells is critical.
In the prior art, the detection of the circulating tumor cells is mainly divided into separation, enrichment and identification analysis of the circulating tumor cells, in the prior art, as the number of CTCs is not large, the clinical detection is commonly carried out by singly drawing 10-20ml blood for clinical detection, and the missed diagnosis is possibly caused, and the separation, enrichment technology of the circulating tumor cells mainly comprises two kinds of technologies: according to the prior art, an automatic circulating tumor cell separator is proposed in CN 214991591U based on an immunological principle separation method and a separation method based on physical characteristics, circulating tumor cells in blood can be effectively separated, automatic and batch detection is carried out, filtering efficiency and detection precision are improved, but when the separator is used, the separation efficiency is low, automatic detection analysis cannot be carried out, and an improvement space exists for identifying the separated tumor cells, so that a plurality of circulating tumor cell single-sampling and automatic identifying machines are proposed to solve the problems proposed in the prior art.
Disclosure of Invention
The invention aims to solve the problems that the separation efficiency is low, automatic detection and analysis can not be carried out, and an improvement space exists for identifying the separated tumor cells in the prior art, and provides a multi-circulation tumor cell single-sampling and automatic identifying machine.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the utility model provides a multiple circulation tumour cell singly adopts and automatic identification machine, includes the identification organism, the side of identification organism is provided with the circulating pump, the inside of identification organism is provided with the separation filter chamber, the inside of identification organism is provided with the removal and promotes the subassembly, the output of removal and promotes the subassembly is provided with the magnetic control chuck that extends to the inside of separation filter chamber, fixed mounting has the adsorption pole in the magnetic control chuck, the inside of identification organism just is located the side of separation filter chamber and is provided with the elution groove;
the inside of appraisal organism is provided with separation detection subassembly, separation detection subassembly is including being located the elution detection groove that the appraisal organism outside set up, the appraisal organism outside is provided with the color development subassembly that is linked together with the elution detection groove.
Further, the two sides of the identification machine body are respectively provided with a guide pipe, and the output end of the circulating pump is provided with a pressure supply pipe communicated with the separation filter cavity.
Further, the inside of appraising organism is offered and is moved the movable groove that promotes the subassembly and correspond, remove the lift assembly and including being located the regulation power frame that the activity set up in the movable groove, the inside fixed mounting in movable groove has the movable rod that corresponds with the regulation power frame, be provided with extension unit on the regulation frame, extension unit's output is provided with the extension pole that is used for flexible.
Further, the end part of the extension rod is fixedly connected with a magnetic control chuck, and at least two groups of extension units, the extension rod and the magnetic control chuck are vertically arranged on the adjusting power frame.
Further, at least two groups of extension units, extension rods and magnetic control chucks are symmetrically distributed on the adjusting power frame in an arch shape, and the number of the elution grooves is consistent with the number of the extension units, the extension rods and the magnetic control chucks and the positions of the elution grooves correspond to the positions of the extension units, the extension rods and the magnetic control chucks.
Further, the elution groove distributes in the both sides of separation filter chamber, adjust power frame and drive lifting unit and adsorption pole and remove to the top of elution groove, the inside of elution groove is provided with the elution pump.
Further, the absorption pole is cylindrical electro-magnet, absorption pole is connected with the magnetic control chuck electricity.
Further, a control panel is arranged on the outer side of the elution detection groove.
Further, the inside of elution detection groove is provided with detection module, detection module is connected with the control panel electricity.
Further, the flow rate of blood inside the separation filter is v=q/a;
meanwhile, the magnetic adsorption force of the adsorption rod is F,
wherein V is the flow rate of blood, Q is the flow rate of blood, and A is the flow area, A1 is the cross-sectional area of the separation filter chamber, A2 is the cross-sectional area of the adsorption rod.
Compared with the prior art, the beneficial effects of this application are:
this multiple circulation tumor cell singly adopts and automatic identification machine, dock two pipes with patient's blood vessel, then through the operation of circulating pump, extract blood and get into the inside of separation filter chamber, the inside of separation filter chamber is provided with the adsorption pole, the even absorption of surface of adsorption pole has antibody to be covered with magnetic bead, the magnetic bead that the antibody was covered can combine together with the marker on tumor cell surface, thereby adsorb the surface at the adsorption pole with tumor cell, reach the effect of filtering tumor cell in the blood, when blood flows through the adsorption pole, can adsorb tumor cell on the adsorption pole, then move into elution detection inslot portion with the adsorption pole through separation detection component and lifting means, then lose magnetism after the adsorption pole outage, be convenient for follow-up carry out elution and dyeing to tumor cell, detect and feed back to control panel at last, through single circulation tumor stem cell of equipment, can design multiple circulation, reach the effect that reduces circulation stem cell, finally can reach the metastasis that reduces cancer cell, reach certain treatment.
Drawings
FIG. 1 is a cross-sectional view of the structure of the present invention;
FIG. 2 is a side cross-sectional view of the present invention;
fig. 3 is a top view of the structure of the present invention.
In the figure: 1. identifying a body; 2. separating the filter chamber; 3. a circulation pump; 4. a conduit; 5. a pressure supply pipe; 6. a magnetic control chuck; 7. an adsorption rod; 8. an elution tank; 9. a movable groove; 10. adjusting a power frame; 11. an extension unit; 12. an extension rod; 13. a moving rod; 14. eluting the detection groove; 15. a color development assembly; 16. a control panel; 17. eluting the pump.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-3, a single picking and automatic identifying machine for multiple kinds of circulating tumor cells comprises an identifying machine body 1, wherein a circulating pump 3 is arranged on the side surface of the identifying machine body 1, a separation filter cavity 2 is arranged in the identifying machine body 1, a movable lifting assembly is arranged in the identifying machine body 1, a magnetic control chuck 6 extending to the inside of the separation filter cavity 2 is arranged at the output end of the movable lifting assembly, an adsorption rod 7 is fixedly arranged in the magnetic control chuck 6, and an elution groove 8 is arranged in the identifying machine body 1 and on the side edge of the separation filter cavity 2.
It should be noted that, in the prior art, because the number of CTCs is not large, clinical detection is generally carried out by singly drawing 10-20ml blood, which may lead to missed diagnosis, and the single sampling method of circulating blood by designing the circulating pump 3 can improve the detection positive rate and the detection precision.
In this embodiment, a separation detection component is disposed inside the identification body 1, the separation detection component includes an elution detection groove 14 disposed outside the identification body 1, and a color development component 15 communicating with the elution detection groove 14 is disposed outside the identification body 1.
The application needs to be emphasized that before using this application, the even absorption of surface of absorption pole 7 has antibody coating magnetic bead, antibody coating magnetic bead can combine together with the marker on tumour cell surface, thereby adsorb tumour cell at the surface of absorption pole 7, reach the effect of filtering tumour cell in the blood, and it is easy to think that can adhere to and use different antibody coating magnetic bead on the absorption pole 7 that is used for filtering of design in this application, be applied to the detection of different grade type circulation tumour cell, and at the in-process of repetition blood circulation, can the efficient multiple circulation tumour cell of separation, detection range for improvement, thereby improve the detection precision and the suitability of equipment.
As shown in the figure, two sides of the identification machine body 1 are respectively provided with a guide pipe 4, the output end of the circulating pump 3 is provided with a pressure supply pipe 5 communicated with the separation filter cavity 2, when the device is used, the guide pipes 4 at two sides can be respectively connected with blood vessels of a user, then the blood of a patient is pumped into the device through the circulating pump 3, and after being subjected to cell filtration, the blood is returned to the body of the patient.
In this embodiment, the movable slot 9 corresponding to the movable lifting assembly is provided in the authentication machine body 1, the movable lifting assembly includes an adjusting power frame 10 movably disposed in the movable slot 9, a movable rod 13 corresponding to the adjusting power frame 10 is fixedly mounted in the movable slot 9, an extension unit 11 is provided on the adjusting frame 10, and an extension rod 12 for telescoping is provided at an output end of the extension unit 11.
In the above, the lifting assembly comprises an adjusting power frame 10 movably arranged in the movable groove 9, two groups of extension units 11 are arranged on the adjusting frame 10, the output ends of the two groups of extension units 11 are provided with extension rods 12 extending to the inside of the separation filter cavity 2, and the extension units 11 can drive the extension rods 12 to extend, so that the adsorption rod 7 at the end part of the extension rods 12 interacts with blood in the filter cavity 2, and tumor cells flowing in the blood are captured by antibody coated magnetic beads on the surface of the adsorption rod 7;
it should be noted that, further, the end of the extension rod 12 is fixedly connected with the magnetic chuck 6, and at least two sets of extension units 11, the extension rod 12 and the magnetic chuck 6 are vertically disposed on the adjusting power frame 10.
Further, two sets of extension unit 11, extension rod 12 and magnetic chuck 6 symmetry are the arch and distribute on adjusting power frame 10, eluting tank 8's quantity and extension unit 11, extension rod 12 and magnetic chuck 6's quantity is unanimous and the position corresponds, simultaneously, eluting tank 8 distributes in the both sides of separation filter chamber 2, adjusting power frame 10 drives lifting unit and adsorption pole 7 and removes to eluting tank 8's top, eluting tank 8's inside is provided with elution pump 17, if set up three filter chamber 2, then set up six adsorption pole 7, when three adsorption pole 7 in the left side are located the filter chamber inside and adsorb the capture of tumour cell, three adsorption pole 7 in the right side are located eluting tank 8's inside and elute, then move about under adjusting power frame 10's drive, circulate continuous elution.
For the convenience of the subsequent detection and treatment of tumor cells, the adsorption rod 7 is a cylindrical electromagnet, the adsorption rod 7 is electrically connected with the magnetic control chuck 6, after the adsorption rod 7 enters the elution groove 8, the adsorption rod 7 can be actively powered off and demagnetized under the control of the magnetic control chuck 6, so that the antibody coating magnetic beads on the outer surface of the adsorption rod 7 fall off, and then the tumor cells are washed to the inside of the elution detection groove 14 under the washing of the elution pump 17 for subsequent detection.
After collecting tumor cells, the step that needs to elute and dye just can be convenient for current check out test set detects, in order to accomplish above-mentioned step, the separation detection subassembly in this application still includes the elution detection groove 14 that is located the inside setting of authentication organism 1, and authentication organism 1 outside is provided with the chromogenic assembly 15 that is linked together with elution detection groove 14.
In this embodiment, the elution detection grooves 14 are distributed on two sides of the movable groove 9, and the adjusting power frame 10 drives the lifting assembly and the adsorption rod 7 to move to the top of the elution detection groove 14.
As shown in fig. 2, the two sets of extension units 11 are driven by the adjusting power frame 10 to alternately enter the elution detection groove 14, and simultaneously the adsorption rod 7 alternately adsorbs and captures tumor cells, and then the tumor cells are sent into the elution detection groove 14 to perform elution and dyeing procedures.
In this embodiment, the inside of the authentication machine body 1 is provided with a moving component capable of driving the adjusting power frame 10 to translate left and right, so as to drive the adsorption rod 7 to ensure the normal operation thereof.
In this embodiment, a control panel 16 is provided outside the elution detection tank 14.
Further, a detection module is provided inside the elution detection tank 14, and the detection module is electrically connected to the control panel 16.
It should be further noted that, the detection module is disposed inside the elution detection slot 14 and is electrically connected to the control panel 16, and as shown in fig. 2, the water supply device and the color development assembly 15 for eluting are disposed inside the identification body 1, and the part of the device related to dyeing and detection is more complete and mature in the prior art, so that the detailed description and description of the device are not provided in this patent.
In order to avoid the situation that the adsorbed antibody coating magnetic beads are washed away due to the fact that the blood flow rate is too high and the magnetic adsorption force of the adsorption rod is insufficient in the using process, the flow rate and the adsorption force of the adsorption rod are limited;
the flow rate of the blood inside the separation filter 2 is v=q/a;
meanwhile, the magnetic adsorption force of the adsorption rod 7 is F,
wherein V is the flow rate of blood, Q is the flow rate of blood, and A is the flow area, A1 is the cross-sectional area of the separation filter chamber, A2 is the cross-sectional area of the adsorption rod.
When the embodiment is used, the two catheters 4 are in butt joint with a blood vessel of a patient, then blood is pumped to enter the separation filter cavity 2 through the operation of the circulating pump 3, the adsorption rod 7 is arranged in the separation filter cavity 2, antibody coated magnetic beads are uniformly adsorbed on the outer surface of the adsorption rod 7, the antibody coated magnetic beads can be combined with markers on the surface of tumor cells, so that the tumor cells are adsorbed on the outer surface of the adsorption rod 7, the effect of filtering the tumor cells in the blood is achieved, when the blood flows through the adsorption rod 7, the tumor cells can be adsorbed on the adsorption rod 7, then the adsorption rod 7 is moved into the elution detection groove 14 through the separation detection assembly and the lifting assembly, then magnetism is lost after the adsorption rod 7 is powered off, the tumor cells are convenient to elute and dye subsequently, and finally detection and feedback to the control panel 16 are carried out.
The invention can achieve the following effects:
1. for the explicitly diagnosed malignant tumor of epithelial origin, whether tumor cells exist in blood circulation or not can be detected by the circulation equipment and the detection equipment provided by the invention, and the metastasis of a patient can be diagnosed earlier than that of an imaging diagnosis;
2. for the metastasis focus with unknown primary focus, the primary focus can be traced by acquiring the circulating tumor cells;
3. the tumor cells in the circulating blood of a tumor patient are collected, adsorbed by the adsorption rod 7 and then transferred into the elution detection groove 14 for elution and detection and then discharged, so that the occurrence of tumor metastasis through blood circulation can be reduced, and the survival rate and prognosis of the tumor of the patient can be improved;
4. the tumor cells enriched by the invention can be further used as a basis and a clinical study, and provide a basis for clinical transformation.
The beneficial effects of the embodiment are as follows:
this multiple circulation tumor cell singly adopts and automatic identification machine, dock two pipe 4 with patient's blood vessel, then through the operation of circulating pump 3, extract blood and get into the inside of separation filter chamber 2, the inside of separation filter chamber 2 is provided with adsorption pole 7, the even absorption of surface of adsorption pole 7 has antibody to be covered with the magnetic bead, the magnetic bead of antibody cladding can combine together with the marker on tumor cell surface, thereby adsorb tumor cell at the surface of adsorption pole 7, reach the effect of filtering tumor cell in the blood, when blood flow passes through adsorption pole 7, can adsorb tumor cell on adsorption pole 7, then through separation detection subassembly and lifting means with adsorption pole 7 shift into elution detection groove 14 inside, then lose magnetism after adsorption pole 7 outage, be convenient for follow-up to tumor cell elute and dye, detect and feed back to control panel 16 at last, through equipment singly adopt circulation tumor stem cell, can design multiple circulation, reach the effect of reducing circulation stem cell, finally can reach the effect of reducing cancer cell, reach certain treatment effect.
The electrical components appearing herein are all electrically connected with the master controller and the power supply, the master controller can be a conventional known device for controlling a computer and the like, and the prior art of power connection is not described in detail herein.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (4)
1. The utility model provides a multiple circulation tumour cell singly adopts and automatic identification machine, includes identification organism (1), its characterized in that: the device is characterized in that a circulating pump (3) is arranged on the side face of the identification machine body (1), a separation filter cavity (2) is arranged in the identification machine body (1), a movable lifting assembly is arranged in the identification machine body (1), a magnetic control chuck (6) extending to the inside of the separation filter cavity (2) is arranged at the output end of the movable lifting assembly, an adsorption rod (7) is fixedly arranged in the magnetic control chuck (6), and an elution groove (8) is formed in the identification machine body (1) and located on the side edge of the separation filter cavity (2);
the detection device is characterized in that a separation detection assembly is arranged in the identification machine body (1) and comprises an elution detection groove (14) arranged on the outer side of the identification machine body (1), and a color development assembly (15) communicated with the elution detection groove (14) is arranged on the outer side of the identification machine body (1);
the two sides of the identification machine body (1) are respectively provided with a guide pipe (4), and the output end of the circulating pump (3) is provided with a pressure supply pipe (5) communicated with the separation filter cavity (2);
the mobile lifting device comprises an identification machine body (1), wherein a movable groove (9) corresponding to a mobile lifting assembly is formed in the identification machine body (1), the mobile lifting assembly comprises an adjusting power frame (10) which is movably arranged in the movable groove (9), a movable rod (13) corresponding to the adjusting power frame (10) is fixedly arranged in the movable groove (9), an extending unit (11) is arranged on the adjusting frame (10), and an extending rod (12) used for extending and retracting is arranged at the output end of the extending unit (11);
the end part of the extension rod (12) is fixedly connected with a magnetic control chuck (6), and the adjusting power frame (10) is vertically provided with at least two groups of extension units (11), the extension rod (12) and the magnetic control chuck (6);
at least two groups of extension units (11), extension rods (12) and magnetic control chucks (6) are symmetrically distributed on the adjusting power frame (10) in an arch shape, and the number of the elution grooves (8) is consistent with the number of the extension units (11), the extension rods (12) and the magnetic control chucks (6) and the positions of the elution grooves are corresponding to the positions of the extension units;
the elution grooves (8) are distributed on two sides of the separation filter cavity (2), the adjusting power frame (10) drives the lifting assembly and the adsorption rod (7) to move to the top of the elution grooves (8), and an elution pump (17) is arranged in the elution grooves (8);
the adsorption rod (7) is a cylindrical electromagnet, the adsorption rod (7) is electrically connected with the magnetic control chuck (6), antibody coated magnetic beads are uniformly adsorbed on the outer surface of the adsorption rod (7), and the antibody coated magnetic beads can be combined with a marker on the surface of a tumor cell, so that the tumor cell is adsorbed on the outer surface of the adsorption rod, and the effect of filtering the tumor cell in blood is achieved; the adsorption rod (7) for filtering can be attached with magnetic beads coated with different antibodies, and is applied to detection of different types of circulating tumor cells.
2. The machine for singulating and automatically identifying a plurality of circulating tumor cells according to claim 1, wherein: a control panel (16) is arranged outside the elution detection groove (14).
3. The machine for singulating and automatically identifying a plurality of circulating tumor cells according to claim 2, wherein: the inside of elution detection groove (14) is provided with detection module, detection module is connected with control panel (16) electricity.
4. A multiple circulating tumor cell singulation and automated identifier according to any one of claims 1-3, wherein: the flow rate of blood inside the separation filter chamber (2) is v=q/a;
at the same time, the magnetic adsorption force of the adsorption rod (7)In the case of F, the number of the components is F,
wherein V is the flow rate of blood, Q is the flow rate of blood, and A is the flow area, A1 is the cross-sectional area of the separation filter chamber, A2 is the cross-sectional area of the adsorption rod.
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| CN202210616326.1A CN114813302B (en) | 2022-06-01 | 2022-06-01 | Multi-circulation tumor cell single sampling and automatic identifying machine |
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| CN202210616326.1A CN114813302B (en) | 2022-06-01 | 2022-06-01 | Multi-circulation tumor cell single sampling and automatic identifying machine |
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| CN102690786B (en) * | 2012-06-05 | 2014-02-12 | 武汉格蓝丽富科技有限公司 | Cell enriching, separating and extracting method and instrument and single cell analysis method |
| CN103725599B (en) * | 2014-01-14 | 2015-05-27 | 刘韬 | Fluid film filter cell separation device |
| CN104548235B (en) * | 2014-10-08 | 2018-01-19 | 广西医科大学 | A kind of circulating tumor cell separates digitization system |
| CN109342720A (en) * | 2018-11-26 | 2019-02-15 | 牛刚 | In body tumor cell enrichment detection device and method |
| CN110170082A (en) * | 2019-04-29 | 2019-08-27 | 刘忠英 | A kind of circulating tumor cell is in body closed loop extract equipment |
| CN112779118A (en) * | 2019-11-07 | 2021-05-11 | 北京机械设备研究所 | Circulating tumor cell forward separation system |
| CN214991591U (en) * | 2020-12-10 | 2021-12-03 | 中国科学院重庆绿色智能技术研究院 | Automatic change circulation tumor cell separating centrifuge |
| CN112891659B (en) * | 2021-01-26 | 2022-08-23 | 南方科技大学 | Wearable circulating tumor cell capturing device and using method and application thereof |
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