CN116148459A - Reading compensation method for chemiluminescent reader - Google Patents

Reading compensation method for chemiluminescent reader Download PDF

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Publication number
CN116148459A
CN116148459A CN202310027942.8A CN202310027942A CN116148459A CN 116148459 A CN116148459 A CN 116148459A CN 202310027942 A CN202310027942 A CN 202310027942A CN 116148459 A CN116148459 A CN 116148459A
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chemiluminescent
reading
temperature
reader
signal value
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CN202310027942.8A
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CN116148459B (en
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倪剑锋
吴骞
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Zhejiang Baotai Intelligent Technology Co ltd
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Zhejiang Baotai Intelligent Technology Co ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence

Abstract

The invention belongs to the field of in-vitro diagnostic instruments, and particularly relates to a reading compensation method for a chemiluminescent reading instrument, which comprises the following steps: step 1, establishing a compensation coefficient for each hole site in a chemiluminescent reader by taking a certain temperature as a reference value; and 2, correcting the signal value of each hole site by using a compensation coefficient when the chemiluminescent reader detects the signal value. According to the invention, by setting the correction coefficient for each hole, the detection accuracy of each hole is improved, and the reading repeatability of the chemiluminescent reader is further improved.

Description

Reading compensation method for chemiluminescent reader
Technical Field
The invention belongs to the field of in-vitro diagnostic instruments, and particularly relates to a reading compensation method for a chemiluminescent reader.
Background
When the 96-well chemiluminescent reader reads each well, the same measuring object is placed in each well, and the read signal values are inconsistent due to the temperature difference in each well, so that the read repeatability among 96 wells is affected, and the detection is not accurate enough.
Disclosure of Invention
In order to make up for the defects of the prior art, the invention provides a technical scheme of a reading compensation method for a chemiluminescent reader, which can reduce the problem of inconsistent reading signal values caused by the temperature difference of each hole and improve the detection repeatability.
A reading compensation method for a chemiluminescent reader, comprising:
step 1, establishing a compensation coefficient for each hole site in a chemiluminescent reader by taking a certain temperature as a reference value;
and 2, correcting the signal value of each hole site by using a compensation coefficient when the chemiluminescent reader detects the signal value.
Further, in the step 1, the compensation coefficient is: s=1+ (T Datum -T i ) N%, where S is the compensation coefficient, T Datum Is a reference value of temperature, T i The temperature value of the ith well is given, and N is the temperature increase value.
Further, the T is Datum 30-40.
Further, the N is 3-10.
Further, in the step 2, the detected signal value is multiplied by a compensation coefficient to obtain a modified signal value.
Compared with the prior art, the invention has the beneficial effects that:
according to the invention, by setting the correction coefficient for each hole, the detection accuracy of each hole is improved, and the reading repeatability of the chemiluminescent reader is further improved.
Drawings
FIG. 1 is a flow chart of the present invention.
Detailed Description
In the description of the present invention, it should be understood that the terms "one end," "the other end," "the outer side," "the upper," "the inner side," "the horizontal," "coaxial," "the center," "the end," "the length," "the outer end," and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, merely to facilitate description of the present invention and simplify the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present invention.
The invention is further described below with reference to the accompanying drawings.
Referring to fig. 1, a reading compensation method for a chemiluminescent reader includes:
and 1, establishing a compensation coefficient for each hole site in the chemiluminescent reader by taking a certain temperature as a reference value.
Wherein, compensation coefficient is: s=1+ (T Datum -T i ) N%, S is compensation coefficient, T Datum For the reference value of the temperature, in the range of 30-40, preferably 37, T i For the temperature value of the ith well, N is a temperature increase value in the range of 3 to 10, preferably 5.
And 2, when the chemiluminescent reader detects the signal value, correcting the signal value of each hole site by using a compensation coefficient, specifically, multiplying the detected signal value by the compensation coefficient to obtain a corrected signal value. The above calculation is performed by a computer.
Examples
The invention is applied to a chemiluminescent immunoassay analyzer in the in-vitro diagnosis industry, taking a 96-hole reader (hole sites are generally 8 rows and 12 columns or 8 columns and 12 rows) as an example, the temperatures of different hole sites are different and generally show that the middle temperature is high and the surrounding temperature is low, and the temperature is related to a temperature gradient effect, the invention obtains the temperature in each hole through software theoretical simulation and actual measurement, and establishes the following temperature map:
1# 2# 3# 4# 5# 6# 7# 8# 9# 10# 11# 12#
A 36.50 36.54 36.58 36.62 36.62 36.62 36.62 36.62 36.62 36.58 36.52 36.40
B 36.80 36.84 36.88 36.92 36.92 36.92 36.92 36.92 36.92 36.88 36.82 36.70
C 37.00 37.04 37.08 37.12 37.12 37.12 37.12 37.12 37.12 37.08 37.02 36.90
D 37.10 37.14 37.18 37.22 37.22 37.22 37.22 37.22 37.22 37.18 37.12 37.00
E 37.10 37.14 37.18 37.22 37.22 37.22 37.22 37.22 37.22 37.18 37.12 37.00
F 37.10 37.14 37.18 37.22 37.22 37.22 37.22 37.22 37.22 37.18 37.12 37.00
G 36.90 36.94 36.98 37.02 37.02 37.02 37.02 37.02 37.02 36.98 36.92 36.80
H 36.60 36.64 36.68 36.72 36.72 36.72 36.72 36.72 36.72 36.68 36.62 36.50
TABLE 1
Then using the formula 1+ (37-T) with 37 ℃ as a reference i ) Correction was performed for each well 5%, T i For the temperature value of each hole, correcting upwards when the temperature is lower than 37 ℃, and correcting downwards when the temperature is higher than 37 ℃, so as to obtain the correction coefficient of each hole:
1# 2# 3# 4# 5# 6# 7# 8# 9# 10# 11# 12#
A 1.025 1.023 1.021 1.019 1.019 1.019 1.019 1.01g 1.019 1.021 1.024 1.030
B 1.010 1.008 1.006 1.004 1.004 1.004 1.004 1.004 1.004 1.006 1.009 1.015
C 1.000 0.998 0.996 0.994 0.994 0.994 0.994 0.994 0.994 0.996 0.999 1.005
D 0.995 0.993 0.991 0.989 0.989 0.989 0.989 0.989 0.989 0.991 0.994 1.000
E 0.995 0.993 0.991 0.989 0.989 0.989 0.989 0.989 0.989 0.991 0.994 1.000
F 0.995 0.993 0.991 0.989 0.989 0.989 0.989 0.989 0.989 0.991 0.994 1.000
G 1.005 1.003 1.001 0.999 0.999 0.999 0.999 0.999 0.999 1.001 1.004 1.010
H 1.020 1.018 1.016 1.014 1.014 1.014 1.014 1.014 1.014 1.016 1.019 1.025
TABLE 2
Correcting the detection signal value of each hole according to the modification coefficient, wherein the signal value data before correction is as follows:
1# 2# 3# 4# 5# 6# 7# 8# 9# 10# 11# 12#
A 61509 60271 61450 61469 61455 61587 61504 61870 61465 62539 61445 61094
B 62528 61991 62554 63178 62581 62830 62531 62870 62574 64086 62561 60482
C 63245 62789 63224 63301 63222 63803 63316 63916 63273 63811 63255 62012
D 63372 62580 63286 63874 63283 64206 63318 63738 63354 63796 63313 61875
E 63316 62931 63303 63325 63331 64263 63391 64083 63325 64134 63313 61293
F 63244 63158 63220 63195 63271 63625 63229 64381 63285 63309 63243 61826
G 63178 62875 63234 63346 63221 63298 63197 64104 63209 63513 63163 62056
H 62241 62473 62273 61590 62175 63429 62183 63263 62217 61526 62193 60977
TABLE 3 Table 3
Wherein mean=62828, sd=884, cv=1.4%.
The corrected signal value data are:
1# 2# 3# 4# 5# 6# 7# 8# 9# 10# 11# 12#
A 63046 61657 62740 62636 62622 62757 62672 63045 62632 63852 62919 62926
B 63153 62486 62929 63430 62831 63081 62781 63121 62824 64470 63124 61389
C 63245 62663 62971 62921 62842 63420 62936 63532 62893 63555 63191 62322
D 63055 62141 62716 63171 62586 63499 62621 63036 62657 63221 62933 61875
E 62999 62490 62733 62628 62634 63556 62693 63378 62628 63556 62933 61293
F 62927 62715 62651 62499 62575 62925 62533 63672 62588 62739 62863 61826
G 63493 63063 63297 63282 63157 63234 63133 64039 63145 63576 63415 62676
H 63485 63597 63269 62452 63045 64317 63053 64148 63088 62510 63374 62501
TABLE 4 Table 4
Wherein mean=62953, sd=534, cv=0.8%.
CV represents repeatability, SD represents standard deviation of 96-well signal values, MEAN represents average value of 96-well signal values, and smaller repeatability cv=sd/MEAN represents better repeatability.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the invention.

Claims (5)

1. A reading compensation method for a chemiluminescent reader, comprising:
step 1, establishing a compensation coefficient for each hole site in a chemiluminescent reader by taking a certain temperature as a reference value;
and 2, correcting the signal value of each hole site by using a compensation coefficient when the chemiluminescent reader detects the signal value.
2. The method according to claim 1, wherein in the step 1, the compensation coefficient is: s=1+ (T Datum -T i ) N%, where S is the compensation coefficient, T Datum Is a reference value of temperature, T i The temperature value of the ith well is given, and N is the temperature increase value.
3. The reading compensation method for chemiluminescent reader of claim 2 wherein the T is Datum 30-40.
4. A method of reading compensation for a chemiluminescent reader of claim 2 wherein N is 3-10.
5. The method according to claim 1, wherein in the step 2, the detected signal value is multiplied by a compensation coefficient to obtain a modified signal value.
CN202310027942.8A 2023-01-09 2023-01-09 Reading compensation method for chemiluminescent reader Active CN116148459B (en)

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