CN116077574A

CN116077574A - Medicine for treating rheumatism bone diseases and preparation method thereof

Info

Publication number: CN116077574A
Application number: CN202211462519.2A
Authority: CN
Inventors: ***
Original assignee: Individual
Current assignee: Individual
Priority date: 2022-11-22
Filing date: 2022-11-22
Publication date: 2023-05-09

Abstract

The invention aims to provide a medicine for treating rheumatism bone diseases, which is prepared from the following raw material medicines in parts by weight: 1-10g of dragon's blood, 1-10g of pseudo-ginseng, 1-10g of peach kernel, 1-10g of safflower, 1-10g of frankincense, 1-10g of myrrh, 1-10g of diverse wormwood herb, 2-12g of Chinese angelica, 2-12g of szechuan lovage rhizome, 1-10g of red paeony root, 1-10g of white paeony root, 2-12g of Chinese teasel root, 2-12g of eucommia bark, 2-12g of Chinese taxillus twig, 2-12g of twotooth achyranthes root, 2-12g of drynaria rhizome, 1-10g of notopterygium root, 1-10g of pubescent angelica root, 1-10g of divaricate saposhnikovia root, 1-10g of cassia twig and 2-12g of papaya. The medicine belongs to a pure traditional Chinese medicine preparation, and has the characteristics of definite curative effect, simultaneous treatment of principal and subordinate symptoms, quick effect, no toxic or side effect, portability, convenient administration and the like through pharmacodynamics and clinical experiment results.

Description

Medicine for treating rheumatism bone diseases and preparation method thereof

Technical Field

The invention belongs to the field of traditional Chinese medicines, and particularly provides a medicine for treating rheumatic bone diseases and a preparation method thereof.

Background

The rheumatic bone disease refers to pain of various bone joints and certain parts of the whole body caused by rheumatism and rheumatoid, and belongs to the category of 'arthralgia syndrome' in traditional Chinese medicine. The theory of traditional Chinese medicine is simple and clear, pain is not easy to get, and the idea is not suitable for treating rheumatism bone diseases. That is, the unsmooth circulation of some parts of the body is caused by some degree of blockage due to some reasons, which is called blockage in jin Kui Yao LX. The current conventional explanation is that blockage caused by wind-cold-dampness arthralgia invading a certain part is a basic cause of the rheumatism bone disease.

The rheumatic bone diseases are frequently seen in degenerative osteoarthropathy, hyperosteogeny, disc bulging, protrusion, ligament calcification, neck, shoulder, waist and leg pain, are frequently seen in clinical common diseases, and are accompanied with the popularization of information-based society, mobile phones and computers, the patients are continuously increased, and the ages are gradually younger. The symptoms are that the muscle joints are distended and trapped with pain, the movement is unfavorable, the cold fatigue is aggravated, the heat rest is relaxed, the clinical treatment method is numerous, the illness state can only be controlled, and the radical cure is difficult. The serious joint deformity, muscular atrophy, joint stiffness, limited functions and movement disorder, and the illness state is continuously aggravated and even disabled with the increase of the age. The quality of life and physical and mental health of the patient are seriously affected.

Along with the further change of people's life habits, the patient number who has the rheumatism osteopathy is further increased more, and the rheumatism osteopathy is the unsmooth condition of blood circulation that causes because of wind-cold, and this disease appears as the swelling of joint even pathological change, and the pathogenesis is slower, but the treatment is also comparatively long, hardly heals to relapse easily. The traditional treatment composition has the advantages that western medicines and traditional Chinese medicines are applied, but the effect is not very obvious.

Disclosure of Invention

The invention aims to provide a medicine for treating rheumatic bone diseases and a preparation method thereof.

The invention particularly provides a medicine for treating rheumatism bone diseases, which is prepared from the following raw material medicines in parts by weight: 1-10g of dragon's blood, 1-10g of pseudo-ginseng, 1-10g of peach kernel, 1-10g of safflower, 1-10g of frankincense, 1-10g of myrrh, 1-10g of diverse wormwood herb, 2-12g of Chinese angelica, 2-12g of szechuan lovage rhizome, 1-10g of red paeony root, 1-10g of white paeony root, 2-12g of Chinese teasel root, 2-12g of eucommia bark, 2-12g of Chinese taxillus twig, 2-12g of twotooth achyranthes root, 2-12g of drynaria rhizome, 1-10g of notopterygium root, 1-10g of pubescent angelica root, 1-10g of divaricate saposhnikovia root, 1-10g of cassia twig and 2-12g of papaya. The optimal proportion is as follows: 6g of dragon's blood, 8g of pseudo-ginseng, 8g of peach kernel, 8g of safflower, 4g of frankincense, 4g of myrrh, 6g of diverse wormwood herb, 10g of Chinese angelica, 10g of szechuan lovage rhizome, 6g of red paeony root, 6g of white paeony root, 10g of common teasel root, 10g of eucommia bark, 10g of Chinese taxillus twig, 10g of twotooth achyranthes root, 10g of drynaria rhizome, 6g of incised notopterygium rhizome, 8g of doubleteeth pubescent angelica root, 8g of divaricate saposhnikovia root, 8g of cassia twig and 10g of common floweringquince fruit.

In the whole formula, three kinds of medicines are classified, namely, blood circulation activating, blood stasis removing and pain relieving medicines; the second category is liver and kidney tonifying and tendons and bones strengthening; and the third type is wind-damp-dispelling arthralgia-relieving. The recipe contains modified formula of ancient recipe "Qili powder", "Duhuosheng decoction" and "Taohong Siwu decoction".

Dragon's blood: dragon's blood (ancient kylin) is a precious medicinal material with blood being as bright red as fire, and is called as "blood-activating holy medicine" in Ming dynasty pharmacy at the time of plum. Sweet, salty and neutral in nature. It enters heart meridian and liver meridian. The functions are as follows: removing blood stasis, relieving pain, stopping bleeding, promoting tissue regeneration and healing sore. The main indications are: traumatic injury, internal injury stagnant pain, dysmenorrhea, postpartum stagnant abdominal pain, traumatic hemorrhage, scrofula, ecthyma, and hemorrhoid.

Pseudo-ginseng: compendium of materia medica Leucon (1765): ginseng is the most precious herb of Ginseng, radix Notoginseng, which has the same flavor as blood and qi tonifying, and so on. Sweet and slightly bitter in flavor and warm in nature. Enter liver and stomach meridians. Efficacy: removing blood stasis, stopping bleeding, detumescence and relieving pain. Can be used for treating hemoptysis, hematemesis, epistaxis, hematochezia, metrorrhagia, traumatic hemorrhage, thoracico-abdominal pain, and traumatic injury.

Eucommia ulmoides: shen nong Ben Cao Jing (Shen nong's herbal meridian) is mainly indicated for lumbago and gonalgia, tonifying middle-jiao, replenishing essence and Qi, strengthening tendons and bones, removing itching and dampness, and dribbling urine. Is suitable for long-term administration, and has the effects of weight reduction and aging resistance. Flavor Gan Weixin, warm nature. Enter the liver and kidney meridians. Efficacy: liver and kidney tonifying, tendons and bones strengthening, and miscarriage preventing effects. The function is as follows: for treating soreness and pain of waist and knees, weakness of feet and knees, itching of yin, fetal movement, and abortion.

Achyranthes root: it is a common traditional Chinese medicine. Shennong Ben Cao Jing (Shen nong's herbal) is listed as the top grade. The pottery Hongdprospect is: its stem has knots like achyranthes root, so it is called. The radix achyranthis bidentatae is one of four major Chinese medicines of Henan, bitter, sweet, sour and flat. It enters liver and kidney meridians. Efficacy: removing blood stasis, dredging channels, nourishing liver and kidney, strengthening tendons and bones, inducing diuresis, treating stranguria, and guiding blood downward. The main indications are: can be used for treating amenorrhea, dysmenorrhea, soreness of waist and knees, weakness of tendons and bones, stranguria, edema, headache, dizziness, toothache, solar sore, hematemesis, and epistaxis.

Notopterygium root: pungent and bitter in flavor and warm in nature. Enter bladder and kidney meridians. The main functions are as follows: dispel exterior cold, dispel wind-damp, promote joint function, and relieve pain. Wind-cold type, headache without sweat, oil-printed cold-dampness arthralgia, edema due to wind-water, sores and ulcers.

Radix angelicae pubescentis): pungent and bitter in flavor and warm in nature. Enter bladder and kidney meridians. The functions are as follows: dispel wind-damp, alleviate pain and relieve exterior syndrome. Can be used for treating arthralgia due to wind-cold-dampness, exterior syndrome due to wind-cold-dampness, headache due to shaoyin, and skin pruritus.

In the recipe, six medicines of dragon's blood, notoginseng, eucommia bark, achyranthes root, notopterygium root and pubescent angelica root are taken as monarch medicines, and have the effects of activating blood circulation, removing blood stasis, dispelling wind, removing dampness and nourishing liver and kidney. Peach kernel, safflower, szechuan lovage rhizome, frankincense, myrrh, cassia twig, mistletoe, drynaria rhizome, divaricate saposhnikovia root, dipsacus root and hosting and lupulus are taken as ministerial drugs, and auxiliary monarch drugs are used for enhancing the effects of dispelling wind-damp, removing blood stasis and relieving pain. Radix Paeoniae Rubra, radix Angelicae sinensis, radix Paeoniae alba, and fructus Chaenomelis can be used as adjuvant and guiding drugs, and can be used for promoting blood circulation, eliminating dampness, relieving pain, and preventing blood heat caused by excessive blood circulation, yin and body fluid injury due to excessive dampness, and excessive dryness.

The invention also provides a preparation method of the medicine for treating the rheumatism bone diseases, and the preparation method of the medicine for treating the rheumatism bone diseases comprises the following steps of: pulverizing 21 raw materials respectively, sieving with 60 mesh sieve, and mixing the sieved powders according to the proportion to obtain oral medicinal powder.

The invention provides a preparation method of a medicine for treating rheumatism bone diseases, which comprises the following steps of: respectively pulverizing 21 raw medicinal materials, sieving with a 60-mesh sieve, mixing the sieved powder according to a proportion to prepare oral medicinal powder, and mixing yellow wine and the oral medicinal powder according to a weight ratio of 0.5:1, preparing a medicine blank, standing for 2-3 days, and then mixing the medicine blank with medical vaseline according to a weight ratio of 1: mixing at a ratio of 1, and making into topical preparation.

The medicine for treating the rheumatic bone diseases provided by the invention can be taken orally or applied externally.

The medicine provided by the invention has proper compatibility of monarch, minister, assistant and guide, mutual assistance, liver and kidney tonifying, blood vessel dredging, cold-dampness dispelling and arthralgia relieving. The medicine has definite curative effect, safety and reliability and efficacy after several generations: promoting reunion of fractured bones, promoting blood circulation, relieving pain, tonifying liver and kidney, dispelling pathogenic wind and removing dampness. Can be used for treating fracture, bone injury and sequelae, cervical spondylosis, lumbar intervertebral disc prolapse, hyperosteogeny, scapulohumeral periarthritis, gonarthritis, gonarthromeningitis, meniscus injury, femur head necrosis, rheumatic and rheumatoid bone pain, etc.

Pharmacodynamic experiments: (exploring the effect of the present drug on the effects of neuropathic pain on the simulated lumbar disc herniation in rats)

1. Material

1.1 animals:

60 female Wistar rats of 12 weeks of age were selected for this experiment, SPF grade, body weight 200-240 g, supplied by Changsheng Biotechnology Co., ltd, lot number SCXK (Liao) 2017-0001.

1.2 drugs and reagents:

the medicine of the invention is brown powder, 15 bags per box, 4.5 g/bag, and 2 times a day.

1.3 environment: animal room of traditional Chinese medicine institute, liaoning province, qualification number: SYXK (Liao) 2007-0010. The temperature of the animal observation chamber is 20-23 ℃ and the relative humidity is 40-60%.

1.4 instrument: von Frey fiber-pain and inflammation research-Shanghai jade research scientific instruments limited; the hot plate pain measurement is BME-410C model, the too transparent glass cage used for the pain measurement is 30cm multiplied by 35cm, and the thickness of the hot plate is 0.5cm. Other devices: syringes, rat cages, feed, water, etc. are supplied by the biological medicine company of gaowei (marhan).

2. Method and results

2.1 experimental method and treatment:

SPF-class male SD rats were fed adaptively at the animal center for 1 week, and weighed into 6 groups of 10 groups each, which were normal, control, model, traditional Chinese, sham, and sham. The intervention factors and application protocol are shown in table 1 below:

TABLE 1 intervention factors and application protocol

2.2 mode of molding:

all rats were modelled by the same experimenter;

model group and traditional Chinese medicine group: after the nerve roots of the left waist 5 and the waist 6 of the rat are exposed, the nerve roots of the waist 5 and the waist 6 of the rat are ligated under direct vision. Mechanical hyperalgesia signs such as plantar eversion and the like, which appear in the rat after ligation, indicate successful molding, and infection is conventionally prevented by penicillin powder after operation.

Sham model group and sham drug administration group: rats were exposed only to the nerve roots of the left lumbar 5, 6, were not ligated, and infection was routinely prevented by penicillin powder after surgery.

The molding was successful in this experiment 4.

2.3 related behavioural detection:

2.3.1 mechanical pain threshold assay (Mechanical withdrawal threshold, MWT):

the von frey test was developed by a pathologist Maximilian von Frey and is a standard method to evaluate mechanical tactile pain in mice and rats against needle punching pain in mechanical stimulus. Mechanical pain threshold measurements of the left lower limb were performed on days 0, 3, 5, 7, and 14 at 10:00 am, respectively, in the experiments. Rats were placed on a 90 x 38cm perforated metal platform and allowed to freely move for 15 minutes. The tip ends of the fiber filaments with different intensities (from small to large) are respectively adopted to stimulate the middle part of the sole of the left hind limb of the rat in the vertical direction, the bottom of the insensitive thick foot is avoided, the measurement is required to be repeated 5 times for each intensity fiber filament, the interval is 3 seconds to 5 seconds each time, 3 times of foot contraction reaction of the rat is caused to be effective intensity, and the minimum fiber filament pressure which can cause the foot contraction reaction of the rat is the mechanical pain threshold.

2.3.2 thermal pain threshold determination:

the rats were freely movable in separate glass boxes after completion of the mechanical pain threshold measurements on days 0, 3, 5, 7, and 14 using an automatic bolometer, and after 15 minutes of adaptation, the paw withdrawal latency of the rats was determined. The intensity of the heat source was maintained at a constant level, and the hind limb and the sole of each rat were alternately measured at intervals of 60 seconds, and after the paw withdrawal latency of the rat was substantially stabilized, the average value of the measurement results obtained from the last 3 times was taken as the basic value of the thermal pain threshold of the rat.

2.3.3 behavioral function score:

rats were observed for movement of the left hind limb according to the Hwang method on day 1, day 3, day 5, day 7 and day 14, respectively. The motor functions are divided into 4 stages: 0 point: the rat can walk normally without movement change; 1, the method comprises the following steps: mild dyskinesia, normal paw withdrawal response to plantar stimulation; 2, the method comprises the following steps: the number of autonomous activities is reduced, the rat can walk normally, has paw withdrawal response to the stimulus of the sole, but is weakened; 3, the method comprises the following steps: and cannot move autonomously.

2.4 statistical treatment:

experimental data are expressed by x+/-s, SPSS 26.0 statistical software is adopted to carry out statistical analysis on the data, paired student t test is adopted for comparison of differences before and after the group, single factor analysis of variance is adopted for comparison of differences among groups, and the difference P is less than 0.05, so that the statistical significance is achieved.

3. Experimental results:

3.1 mechanical pain threshold comparison of rats at different time points (as in table 2):

there was no significant difference in mechanical pain threshold (P > 0.05) for each group of rats at day 0 of the experiment, and after modeling, the mechanical pain threshold was gradually decreased for the 4 groups (a slight decrease in mechanical pain threshold for the 2 sham-operated groups may be associated with stimulation of the peri-nerve during surgery, resulting in peri-nerve edema associated with inflammation).

On day 3 of the experiment, the mechanical pain threshold of the 2 spinal nerve ligation groups was significantly reduced, and the difference compared with the normal group was statistically different (P < 0.05); compared with the traditional Chinese medicine group for spinal nerve ligation, the spinal nerve ligation model group has no obvious difference (P is more than 0.05); the false operation model group is compared with the traditional Chinese medicine group for the false operation, no obvious difference (P is more than 0.05), the 2 spinal nerve ligation groups are compared with the 2 false operation groups, the mechanical pain threshold of the 2 spinal nerve ligation groups is obviously reduced, and the difference has statistical significance (P is less than 0.05).

On day 5 of the experiment, the mechanical pain threshold of the 2 spinal nerve ligation groups was gradually increased, and no obvious difference (P > 0.05) was seen compared with the two groups; the mechanical pain threshold of the 2 false operation groups is slightly reduced, and compared with the traditional Chinese medicine group for the false operation and the model group for the false operation, no obvious difference (P is more than 0.05) is found; compared with 2 artificial operation groups, the mechanical pain threshold of the spinal nerve ligation group is obviously reduced, and the difference has statistical significance (P is less than 0.05).

On the 7 th day of the experiment, the traditional Chinese medicine group for spinal nerve ligation is compared with the model group for spinal nerve ligation, the mechanical pain threshold of the traditional Chinese medicine group for spinal nerve ligation is obviously increased, and the difference has statistical significance (P is less than 0.05); the mechanical pain threshold of the 2 sham groups is slightly raised, and compared with the two, no obvious difference (P is more than 0.05) is found; compared with 2 artificial operation groups, the mechanical pain threshold of the spinal nerve ligation group is obviously reduced, and the difference has statistical significance (P is less than 0.05).

On the 14 th day of the experiment, the spinal nerve ligation model group is compared with the spinal nerve ligation traditional Chinese medicine group, the mechanical pain threshold of the spinal nerve ligation traditional Chinese medicine group is obviously increased, and the difference has statistical significance (P is less than 0.05); compared with the normal group, the mechanical pain threshold of the spinal nerve ligation traditional Chinese medicine group is still lower than that of the normal group, and the difference has statistical significance (P is less than 0.05); compared with 2 artificial operation groups, the mechanical pain threshold of the spinal nerve ligation traditional Chinese medicine is lower than that of the 2 artificial operation groups, and the difference has statistical significance (P is less than 0.05); compared with a false operation traditional Chinese medicine group and a false operation model group, the mechanical pain threshold of the false operation traditional Chinese medicine group is slightly raised, and the difference has statistical significance (P is less than 0.05); the traditional Chinese medicine in the sham operation has no obvious difference (P is more than 0.05) compared with the normal group.

Table 2 mechanical pain threshold comparison for rats of each group

Note that: a represents that P is more than 0.05 compared with the prior molding, and b represents that P is less than 0.05 compared with the prior molding.

3.2 comparison of thermal pain threshold of rats at different time points (as in Table 3)

There was no significant difference in thermal pain threshold for each group of rats at day 0 of the experiment (P > 0.05), and after modeling, the thermal pain threshold was gradually decreased for the spinal nerve ligation group and the sham surgery group (slight decrease in thermal pain threshold for sham surgery group may be associated with stimulation of the nerve periphery during surgery, resulting in edema and inflammation around the nerve).

On experiment day 3, the thermal pain threshold of the spinal nerve ligation group was significantly reduced, and the difference compared with the normal group was statistically different (P < 0.05); compared with the traditional Chinese medicine group for spinal nerve ligation, the spinal nerve ligation model group has no obvious difference (P is more than 0.05); the sham operation group has slightly reduced thermal pain threshold compared with the normal group, and the difference has statistical significance (P is less than 0.05); compared with the sham operation traditional Chinese medicine group, the sham operation model group has no obvious difference (P is more than 0.05), and compared with the sham operation group, the sham operation model group has obviously reduced thermal pain threshold, and the difference has statistical significance (P is less than 0.05).

On experiment day 5, the thermal pain threshold of the spinal nerve ligation traditional Chinese medicine rises, but is still lower than that of the normal group, and the difference has statistical significance (P is less than 0.05); compared with a spinal nerve ligation traditional Chinese medicine group and a spinal nerve ligation model group, the thermal pain threshold of the spinal nerve ligation traditional Chinese medicine group is obviously increased, and the difference has statistical significance (P is less than 0.05); the thermal pain threshold of the sham operation group is slightly raised, and compared with the sham operation model group, the sham operation traditional Chinese medicine group has no obvious difference (P is more than 0.05); compared with the sham operation group, the spinal nerve ligation group has obviously reduced thermal pain threshold, and the difference has statistical significance (P is less than 0.05).

On the 7 th day of the experiment, the thermal pain threshold of the traditional Chinese medicine group for spinal nerve ligation is further increased, compared with the traditional Chinese medicine group for spinal nerve ligation, the thermal pain threshold of the traditional Chinese medicine group for spinal nerve ligation is obviously increased, and the difference has statistical significance (P is less than 0.05); the sham operation group has slightly reduced thermal pain threshold compared with the normal group, and the difference has statistical significance (P is less than 0.05); compared with the traditional Chinese medicine group for the false operation, no obvious difference (P is more than 0.05) is found; compared with the sham operation group, the spinal nerve ligation group has obviously reduced thermal pain threshold, and the difference has statistical significance (P is less than 0.05).

On the 14 th day of the experiment, the thermal pain threshold of the traditional Chinese medicine group for spinal nerve ligation slightly rises, compared with the traditional Chinese medicine group for spinal nerve ligation, the thermal pain threshold of the traditional Chinese medicine group for spinal nerve ligation obviously rises, and the difference has statistical significance (P is less than 0.05); compared with the normal group, the thermal pain threshold of the spinal nerve ligation traditional Chinese medicine group is still lower than that of the normal group, and the difference has statistical significance (P is less than 0.05); compared with a sham operation group, the heat pain threshold of the spinal nerve ligation group is lower than that of the sham operation group, and the difference is statistically significant (P is less than 0.05); the sham operation group has thermal pain threshold lower than that of the normal group, and the difference has statistical significance (P is less than 0.05); compared with the sham operation model group, the sham operation traditional Chinese medicine group has no obvious abnormality (P is more than 0.05).

Table 3 thermal pain threshold comparison for rats of each group

3.3 comparison of behavioral function scores of the left hind limbs of rats at different time points (as in Table 4)

The behavioral function scores of the rats in each group on the 0 th day of the experiment are not obviously different (P is more than 0.05), after modeling, the motor function of the spinal nerve ligation group is gradually reduced, and the slight reduction of the left hindlimb function of the sham operation group can be related to edema and inflammation around nerves caused by operation.

On the 3 rd day of the experiment, the spinal nerve ligation group showed a significant decrease in motor function, and the difference was statistically significant (P < 0.05) compared to the normal and sham operation groups; compared with the traditional Chinese medicine group for spinal nerve ligation, the difference has no statistical significance (P is more than 0.05); compared with the normal group, the false operation group has slightly reduced movement function, and the difference has statistical significance (P is less than 0.05); the difference has no statistical significance (P > 0.05) compared with the sham operation model group.

On the 5 th day of the experiment, the motor function of the spinal nerve ligation group is improved, compared with the normal group, the motor function score of the spinal nerve ligation group is obviously higher than that of the normal group, and the difference has statistical significance (P is less than 0.05); the difference between the spinal nerve ligation model group and the spinal nerve ligation traditional Chinese medicine group is not statistically significant (P is more than 0.05); the motor function of the false operation group is improved, and compared with the normal group, no obvious difference (P is more than 0.05) is seen; compared with the prior model, no obvious difference (P is more than 0.05), the artificial operation model group is compared with the artificial operation traditional Chinese medicine group, the spinal nerve ligation group is not obviously different (P is more than 0.05), the movement function is obviously reduced compared with the artificial operation group, and the difference has statistical significance (P is less than 0.05).

On the 7 th day of the experiment, the motor function of the group of the spinal nerve ligation traditional Chinese medicine is improved earlier, and compared with the normal group, the difference has statistical significance (P is less than 0.05); compared with the spinal nerve ligation model group, the spinal nerve ligation traditional Chinese medicine group has obviously improved motor function and statistically significant difference (P is less than 0.05); compared with a sham operation group, the spinal nerve ligation group has obviously reduced motor function, and the difference has statistical significance (P is less than 0.05); no obvious abnormalities in motor function were seen in the sham group.

On the 14 th day of the experiment, the motor function of the traditional Chinese medicine group for spinal nerve ligation is obviously improved, and compared with a spinal nerve ligation model, the difference has statistical significance (P is less than 0.05); compared with the normal group and the sham operation group, the spinal nerve ligation group has reduced motor function, and the difference has statistical significance (P is less than 0.05).

Table 4 comparison of hind limb behavioural function scores for rats of each group

Note that: a represents that P is more than 0.05 compared with the prior molding, b represents that P is less than 0.05 compared with the prior molding

4. Conclusion:

in summary, the effect of the drug of the present invention on pain behavior of spinal nerve ligation rats: can inhibit mechanical hyperalgesia and thermal hyperalgesia of rat with spinal nerve ligation model, and improve motor function of rat with spinal nerve ligation model.

Clinical trial (research on the curative effect of the medicine for treating cold-dampness type lumbar disc herniation)

1. Clinical data

1.1 case Source

120 patients with cold-dampness type Lumbar Disc Herniation (LDH) meeting research standards are selected as clinical study objects from hospitalized patients admitted to bone department of a hospital affiliated to the university of traditional Chinese medicine of Liaoning from 9 months 2021 to 9 months 2022.

1.2 diagnostic criteria

1.2.1 Western diagnostic criteria

Reference documents and 2021, chinese diagnosis and treatment guidelines for lumbar disc herniation, the specific diagnosis criteria are as follows:

(1) repeated lumbago with history of lumbar trauma or strain;

(2) lumbar pain is accompanied by local limitation of movement, lumbar physiological curvature becomes shallow, disappears, and lumbar rigidity or compensatory lateral curvature appears;

(3) lumbago is accompanied with radioactive radicular pain of buttocks and lower limbs, symptoms are aggravated during activities, and the pain can be relieved after rest;

(4) abnormal skin feel, muscle strength, physiological reflex and pathological reflex, and atrophy of muscles;

(5) the pathological segment adjacent to the vertebrae or the spinous process has different degrees of tenderness and click pain, and the crural nerve traction test, the straight leg elevation test and the reinforcement test are positive;

(6) imaging diagnostic criteria: the positive lumbar vertebra X-ray lateral position sheet prompts the lumbar vertebra to have shallow physiological radian, narrow intervertebral space or uneven left and right, and lateral curvature of the spine. Lumbar vertebra CT or MRI prompts lumbar intervertebral disc tissues to protrude into the vertebral canal to different degrees, and affected segment dominant regions conform to the symptoms and signs of patients. LDH is determined by meeting any two of the first 5 criteria, plus the imaging diagnostic criteria of (6).

1.2.2 diagnostic criteria for TCM

Referring to the traditional Chinese medicine syndrome type of LDH in traditional Chinese medicine syndrome diagnosis curative effect standard: syndrome of cold dampness: cold pain in the waist and lower extremities is severe, the side turning is unfavorable, the pain is fixed, the pain increases when encountering cold, the pain is relieved, and the limbs cool. A pale and fat tongue with white or greasy coating and a wiry, slow or deep and tight pulse.

1.3 inclusion criteria

(1) Meets the Western diagnosis standard of LDH;

(2) meets the traditional Chinese medicine syndrome typing standard of LDH;

(3) the age is between 45 and 75 years old, and the sex is unlimited;

(4) smooth communication, and can be used for listening to various treatments, examinations and scores of doctors;

(5) no other treatment or other medication was taken for the last two months;

(6) patients with lumbar surgery history and non-lumbar surgery indications in the past;

(7) the patients had informed consent for the study, voluntarily attended and signed the relevant informed consent.

1.4 exclusion criteria

(1) Patients diagnosed with LDH not due to cold dampness

(2) Patients who had difficulty in communication and were unable to conduct the study independently;

(3) patients with serious spinal diseases such as spinal trauma, spinal tumor, lumbar spinal stenosis, lumbar tuberculosis, lumbar spondylolisthesis, etc.;

(4) the lumbar vertebra operation is performed, or the patients with paralysis of lower limbs, dysuria, spinal cord and cauda equina nerve injury exist at present, the operation indication is met, and the illness state cannot be prolonged;

(5) patients with serious heart, brain, liver, kidney and other viscera dysfunction and severe basic diseases such as peptic ulcer and coronary heart disease;

(6) the patients are allergic constitution and allergic to the drugs used in the study;

(7) patients who are participating in other studies or using other treatments and medications.

2. Research method

2.1 case grouping

120 cold-dampness type LDH patients are divided into a lumbago relieving group, a arthralgia relieving capsule group, a compound pain relieving capsule group and an observation group by a random number table method, wherein 30 patients are respectively in each group.

2.2 sources of drugs

Capsule for treating arthralgia, approval document: chinese medicine standard Z10960010, manufacturer: bohai pharmaceutical Congress, smoke counter, specification: 0.31 g/grain.

Compound pain capsule, approval document: national drug standard Z20073054, manufacturing enterprise: the Gansu province western peak pharmacy Limited liability company, specification: 0.3 g/grain.

Lumbago relieving capsule, approval document: national drug standard Z13020898, manufacturing enterprise: jingfukang pharmaceutical Co., ltd., specification: 0.3 g/grain.

2.3 methods of treatment

The 4 groups are all given with indiscriminate conventional treatment ((1) bed rest, (2) waist wear during the activity of getting up, and (3) five-point support and small swallow lumbar muscle exercise). The observation group was given the drug of the present invention on the basis of conventional treatment (2 times daily oral administration, 1 bag each time, water-infusion administration, 4 weeks continuous administration); the arthralgia-relieving capsule group is prepared by the arthralgia-relieving capsule (orally taken for 2 times a day, 1 granule each time, and 4 weeks continuously); the compound pain capsule group is subjected to compound pain capsule (3 times daily oral administration, 3 granules each time, 4 weeks continuously) on the basis of conventional treatment; lumbago relieving capsule (3 capsules for oral administration for 2 times daily for 4 weeks).

2.4 method of viewing

2.4.1 observations index

These four time nodes evaluate the patient's VAS score, JOA score, ODI index, and chinese medical science symptoms score before treatment, at 2 weeks of treatment, at 4 weeks of treatment, and 1 month after treatment. 2.4.1 visual simulation pain scoring (VAS scoring)

The pain level of the patient was assessed using a visual simulated pain score. The visual analog pain score is also known as VAS score, and a doctor draws a transverse line about 10 cm long on a piece of paper, one end of which is marked as 0, which represents no pain; the other end is marked as 10 and represents severe pain; the patient is prescribed to mark the extent of pain on the transverse line based on the subjective feeling. The 0-3 area indicates mild pain, the 4-6 area indicates moderate pain, and the 8 area indicates severe pain.

2.4.2 evaluation of treatment score by Japanese society of orthopedics (JOA score)

The evaluation of the treatment score by the Japan orthopedics society is mainly used for evaluating the degree of human dysfunction, the evaluation of the treatment score by the Japan orthopedics society is also called JOA score, and the quantitative score is carried out on the degree of lesions of 6 aspects of lower back pain, leg pain and the like of a patient according to the illness state of the patient and related examination bodies. The highest score of 29 points is the lower the final score, which represents the more severe the overall dysfunction of the lumbar spine.

2.4.3 Osweary dysfunction index questionnaire (ODI index)

The Osweary dysfunction index questionnaire is used for evaluating the degree of limb dysfunction, also called ODI index, and quantitatively scoring 10 aspects of the biological lumbago and leg pain degree of a patient, the personal life cooking condition and the like according to the illness state of the patient and related examination bodies, wherein each content is 5 points at most, the total score is 50 points at most, and the higher the numerical value is, the heavier the limb dysfunction is.

2.4.4 traditional Chinese medical Condition scoring

The Chinese medicine symptoms are scored according to the main symptoms and the activity degree of cold pain and numbness of the waist and legs, coldness of limbs, tongue appearance and 4 secondary symptoms of the pulse condition of a patient, wherein the main symptoms are at most 6 points, the secondary symptoms are at most 3 points, the total score is 18 points, and the higher the score is, the heavier the Chinese medicine symptoms of the patient are represented.

2.5 safety evaluation and adverse reaction event Observation

If any drug allergy, drug intolerance or other adverse reactions occur in the clinical test process of the patient, the patient needs to make records and timely perform symptomatic treatment, and if necessary, stopping the drug and stopping the treatment.

2.6 compliance supervision

Because the treatment and observation time is long, the compliance of patients is required to be remotely supervised through various modes such as telephone, weChat and the like, and if the compliance is not strong in the experimental process, the patients who finish the study cannot be adhered to, and records are required to be made in time.

2.7 statistical analysis

Clinical data were collected and consolidated into Excel forms. The collated clinical data were analyzed using statistical software SPSS 22.0. The metering data are unified in the form of mean value +/-standard deviation (x+/-s), and the normal distribution characteristics and variance homogeneity test are met; and checking the counting data row chi square. After all data are analyzed and compared, P is less than 0.05 to indicate that the difference has statistical significance.

3. Results of clinical study

3.1 analysis of comparative equality between groups of four patients prior to treatment

To rule out the effect of the patient's general condition on the overall clinical efficacy, the age, sex and course of the 4 groups of patients were compared separately prior to the study. Meanwhile, the VAS score, JOA score, ODI index and traditional Chinese medicine syndrome score evaluated before treatment of 4 groups are compared in balance, and whether the 4 groups are comparable or not is determined, and then the next clinical treatment plan is executed.

3.1.1 comparison of the ages of four groups of patients

Age distribution of four groups of patients: the age of the observation group (64.07.+ -. 6.37) was 75 years maximum and 51 years minimum; the age of the general arthralgia group (64.82+/-6.38) is 75 years at maximum and 50 years at minimum; age of compound pain group (64.25+ -6.35), maximum age of 76 years, minimum age of 50 years; lumbago relieving group age (64.61 + -6.36), 75 years maximum and 49 years minimum. Through t test, P is more than 0.05, which indicates no obvious difference and comparability.

3.1.2 comparison of the sex of four groups of patients

Sex distribution of four groups of patients: group 22 men and 8 women were observed; 20 men and 10 women in the arthralgia-relieving group; 23 men and 7 women in the compound pain group; lumbodynia is relieved for 22 men and 8 women. Through t test, P is more than 0.05, which indicates no obvious difference and comparability.

3.1.3 comparison of the course of four groups of patients

Patient course distribution for four groups: observing the disease course (3.51+/-1.43) of the group for 6 years at the longest and 1 year at the shortest; the disease course of the arthralgia-syndrome-relieving group (3.58+/-1.66) is 7 years at the longest and 1 year at the shortest; the disease course of the compound pain group (3.55+/-1.61) is 7 years at the longest and 1 year at the shortest; the disease course of the low back pain relieving group (3.53+/-1.49) is 6 years at the longest and 2 years at the shortest. Through t test, P is more than 0.05, which indicates no obvious difference and comparability.

3.1.4 pre-treatment VAS score, JOA score, ODI index comparison for four groups of patients

Four groups of patients were treated for pre-VAS score, JOA score, ODI index profile:

the score of VAS (6.29+/-1.44) in the group before treatment, the score of VAS (6.21+/-1.62) in the group after treatment, the score of VAS (6.25+/-1.53) in the group after compound pain, the score of VAS (6.23+/-1.59) in the group after treatment, and the P of the group after t test is more than 0.05, which indicates that the four groups have no obvious difference. The score of JOA (12.14+/-1.60) for the pre-treatment observation group, JOA (12.21+/-1.71) for the general pain group JOA (12.17+/-1.66) and JOA (12.15+/-1.59) for the low back pain group, and no obvious difference is indicated by t-test, P > 0.05. The ODI index (39.29+ -3.18) of the group and the ODI index (39.11+ -3.20) of the group are observed before treatment, the ODI index (39.22+ -3.17) of the compound pain group and the ODI index (39.13 + -3.15) of the group are observed, and the P is more than 0.05 after t test, which indicates no obvious difference.

3.1.5 comparison of traditional Chinese medicine Condition score before treatment for four groups of patients

Traditional Chinese medicine syndrome score before treatment of four groups of patients: observing the Chinese medicine syndrome score (16.54+/-1.48) of the group before treatment, the Chinese medicine syndrome score (16.32+/-1.34) of the general arthralgia group, the Chinese medicine syndrome score (16.41+/-1.41) of the compound pain group, the Chinese medicine syndrome score (16.49+/-1.39) of the low back pain group, and carrying out t test, wherein P is more than 0.05, so that no obvious difference is indicated.

3.2 analysis of efficacy

3.2.1 comparison of VAS score after treatment for four groups of patients

VAS score distribution:

when the treatment is carried out for 2 weeks, the observation group is (4.66+/-1.20) score, the arthralgia-relaxing group is (4.71+/-1.08) score, the compound pain group is (4.69+/-1.15) score, the lumbago relieving group is (4.73+/-1.13) score, and through t test, P is more than 0.05, the four groups of VAS scores are not obviously different;

at 4 weeks of treatment, the observation group is (3.27+/-0.96) score, the general arthralgia group is (3.85+/-0.97) score, the compound pain group is (3.88+/-1.03) score, the lumbago pain group is (3.79+/-0.95) score, and through t test, P is less than 0.05, the difference between the VAS scores of the observation group and other 3 groups is prompted;

1 month after treatment, the observation group is (2.05+/-0.52) score, the arthralgia-relieving group is (2.74+/-0.68) score, the compound pain group is (2.88+/-0.83) score, the lumbago relieving group is (2.79+/-0.75) score, and the P is less than 0.05 after t test, so that the difference between the VAS scores of the observation group and other 3 groups is prompted;

when the treatment is carried out for 2 weeks, the VAS scores of the 4 groups of patients are lower than those before the treatment, the score difference is not obvious, and the 4 medicines can be used for relieving the pain of the patients; the VAS score of the observed group was lower than that of the other 3 groups for 4 weeks and 1 month after the treatment was completed, and considering that the drug of the present invention had a certain advantage in improving pain as compared with the 3 control group drugs.

Score comparison of JOA after treatment for four groups of 3.2.2 patients

JOA score distribution:

when the treatment is carried out for 2 weeks, the observation group is (15.11+/-2.10) score, the arthralgia-relieving group is (14.71+/-2.14) score, the compound pain group is (14.75+/-2.12) score, the lumbago relieving group is (14.79+/-2.05) score, and through t test, P is more than 0.05, the scores of the patients JOA in the 4 groups are not obviously different;

at 4 weeks of treatment, the observation group was (20.21+ -1.62) divided into (18.57+ -1.97) and the compound pain group was (18.88+ -1.83) divided into (18.79+ -1.75) and the lumbago group was t-checked, and P < 0.05, the observation group was prompted to have a difference in JOA scores from the other 3 groups of patients;

1 month after treatment, the observation group is (23.14+/-1.27), the arthralgia-relieving group is (21.07+/-1.65), the compound pain group is (21.88+/-1.83), the lumbago relieving group is (21.79+/-1.75), and the P is less than 0.05 after t test, so that the observation group and other 3 groups of patients are prompted to have different scores of JOA;

at 2 weeks of treatment, the JOA score of the 4 groups of patients is higher than that before treatment, the score difference is not obvious, and the 4 drugs can be used for improving lumbar vertebra functions of the patients; the scores of JOA are different between 4 groups of patients after treatment for 4 weeks and 1 month after treatment, and the medicine has certain advantages compared with other 3 control group medicines in the aspect of improving lumbar function.

3.2.3 ODI index comparison after treatment for four groups of patients

ODI index distribution case:

at 2 weeks of treatment, the observation group is (35.14 + -3.08), the arthralgia-relieving group is (36.14 + -2.68), the compound pain group is (36.32+ -2.85), and the lumbago relieving group is (36.09+ -2.91); through t test, P is more than 0.05, and 4 groups of patients are indicated to have no significant difference in ODI indexes;

at the treatment time of 4 weeks, the observation group is (30.69+/-1.87), the arthralgia-relieving group is (30.82 +/-2.07), the compound pain group is (30.12+/-2.03), the lumbago relieving group is (29.98+/-1.95), and the P is more than 0.05 after t test, so that 4 groups of patients are prompted that the ODI indexes have no significant difference;

1 month after treatment, the observation group is (24.86+/-0.93) divided, the control group is (26.71+/-1.18) divided, the compound pain group is (27.02+/-1.03) divided, the lumbago relieving group is (26.98+/-1.05) divided, and through t test, P is less than 0.05, the difference between ODI indexes of the observation group and other 3 groups of patients is prompted;

the ODI indexes of the 4 groups of patients are lower than those before treatment in 2 weeks and 4 weeks after treatment, the score difference is not obvious, and the 4 drugs can improve the limb functions of the patients; the ODI index between 4 groups of patients is different 1 month after the treatment is finished, and the medicine has certain advantages compared with other 3 control group medicines in the aspect of improving the limb functions.

3.2.4 comparison of traditional Chinese medicine Condition score after treatment of four groups of patients

The condition of scoring distribution of traditional Chinese medicine symptoms:

when the treatment is carried out for 2 weeks, the observation group is (14.29+/-1.38) and the arthralgia-relieving group is (14.54+/-1.35), the compound pain group is (14.36+/-1.31), the lumbago relieving group is (14.40+/-1.33), and the P is more than 0.05 after t test, so that no obvious difference exists in the traditional Chinese medicine syndrome scores of 4 groups of patients;

when the treatment is carried out for 4 weeks, the observation group is divided into (12.08+/-0.98), the arthralgia-relieving group is divided into (12.61+/-1.03), the compound pain group is divided into (12.66+/-1.11), the lumbago relieving group is divided into (12.64+/-1.03), the traditional Chinese medicine syndrome score of the observation group is lower than that of the other 3 groups, and the T test shows that the P is less than 0.05, so that the difference exists between the traditional Chinese medicine syndrome scores of the observation group and the traditional Chinese medicine syndrome scores of other 3 groups of patients;

1 month after treatment, the observation group is (6.79+/-0.88), the general arthralgia group is (7.57+/-0.88), the compound pain group is (7.66+/-0.81), the lumbago relieving group is (7.64+/-0.83), the traditional Chinese medicine syndrome score of the observation group is lower than that of the other 3 groups, and through t test, P is less than 0.05, and the observation group is prompted to have difference with the traditional Chinese medicine syndrome scores of other 3 groups of patients;

when the treatment is carried out for 2 weeks, the traditional Chinese medicine syndrome score of the 4 groups of patients is lower than that before the treatment, the 4 medicines are indicated to be capable of improving the cold-dampness syndrome related syndrome of the patients, but the traditional Chinese medicine syndrome score difference among the 4 groups of patients is not obvious, the treatment time is considered to be shorter, the traditional Chinese medicine efficacy is slow to play, and the difference is not obvious; the medicine gradually shows advantages in improving the cold-dampness type LDH symptoms after treatment for 4 weeks and 1 month after treatment.

3.3 evaluation of safety

The two groups of patients have no drug allergy and obvious adverse reaction in the clinical test process, and the treatment scheme is high in safety and worthy of clinical popularization.

3.4 compliance description

The two groups of patients have good compliance in the clinical trial process and are basically completely matched with the whole-course treatment.

4. Conclusion(s)

The medicine for treating lumbar disc herniation is compared with a "Tongbi capsule", "Compound Shangtong capsule" and "Yaotongning capsule" which are clinically applied for many years and have definite curative effects, and the medicine has better curative effects (P is less than 0.05) on pain, lumbar vertebra function, lower limb function and traditional Chinese medicine syndrome improvement of patients with lumbar disc herniation.

Case one:

huang Mou, female, 55 years, date of first visit: 11.10.2019.

Complaints: lumbago is caused by more than two months.

The current medical history: the patients have no obvious cause of lumbago before two months, and the lumbago symptoms such as the capsules of six ingredients including rehmannia and lumbago are not obviously improved by self-purchasing, and the patients can visit the patients at random. The symptoms are expressed by 'fear sleep, sleep awakening fatigue' and dizziness, dry mouth, stomach discomfort, cool lower abdomen, easy abdominal distension, frequent sighing, normal urine, and dry stool in 2 days. Pale tongue with white and greasy coating, marked teeth on the sides of the tongue and slow pulse.

Physical examination: the waist has unfavorable movement, forward bending of 70 degrees, backward extending of 15 degrees, left bending of 10 degrees and right bending of 15 degrees; lower limb straight leg elevation test (+).

Diagnosis of traditional Chinese medicine: lumbago (cold-dampness stagnation type).

Syndrome analysis: cold-dampness stagnation, pain in the waist due to obstruction of the channels and collaterals, damp obstruction of middle energizer, spleen failing to transport, chest distress, gastric fullness, vomiting, anorexia, tiredness, debilitation, and loose stool. Pale tongue with white and greasy coating and deep pulse are symptoms of cold-dampness retention.

The method for treating the traditional Chinese medicine comprises the following steps: dispel cold and remove dampness, unblock collaterals and relieve pain.

Prescription drug: the medicine of the invention is taken with 4.5g warm water 2 times a day, and the medicine and the Vaseline are prepared into paste for external application and affected part.

Re-diagnosis for 11 months and 17 days:

the lumbago is improved, the sleep quality is improved, the pain sensation of cervical vertebra is relieved, the abdominal distention disappears, and the tongue pulse is similar to that before. The treatment regimen was as before, and self-healed after 1 month.

Case two:

chen Mou, men, 25 years, date of first visit: 9 months and 15 days 2020.

Complaints: lumbago is accompanied by lower left limb radiation pain for 3 days, and aggravates for 1 day.

The current medical history: the patient has lumbago after 3 days of exercise, with lower left limb pain, the above symptoms are not improved after self rest, and the above symptoms are aggravated by cold waist at yesterday, and the patient is treated at random. The symptoms are lumbago, pain in the left lower limb, constipation, pale tongue with thin and white coating and slow pulse.

Physical examination: the waist has unfavorable movement, forward bending of 40 degrees, backward extending of 5 degrees, left bending of 10 degrees and right bending of 15 degrees; left lower limb straight leg elevation test (+), 1.5cm pressure pain (+) is bypassed by the waist L5.

Syndrome analysis: the trauma causes blood stasis to block collaterals, and cold invasion causes pain due to obstruction of lumbar channels and collaterals.

The method for treating the traditional Chinese medicine comprises the following steps: dispelling cold, removing blood stasis, dredging collaterals and relieving pain.

Re-diagnosis for 9 months and 22 days:

the symptoms of lumbago and lumbar movement disorder are obviously improved, the symptoms of the radioactive pain of the left lower limb are improved as compared with the front symptoms, and the treatment scheme is as before, and the patients can self-heal after 2 weeks.

Case three:

li Mou, men, 30 years, date of first visit: 8 months and 15 days 2020.

Complaints: lumbago is more than 1 month, and aggravate for 1 day.

The current medical history: the patient has no obvious cause of lumbago before 1 month, the symptoms are not improved after self rest, the symptoms are aggravated by the cold waist of the patient yesterday, and the patient visits at random. The symptoms are lumbago, pain in the left lower limb, constipation, pale tongue with thin and white coating, and deep and tight pulse.

Physical examination: the waist has unfavorable movement, forward bending of 40 degrees, backward extending of 5 degrees, left bending of 10 degrees and right bending of 15 degrees; left lower limb straight leg elevation test (+), 1.5cm tenderness left by lumbar region L3,4, 5.

Syndrome analysis: a pale tongue with thin and white coating and a deep and tense pulse are the cold-affected phases, and cold invasion causes pain due to obstruction of the lumbar channels.

Re-diagnosis for 8 months and 22 days:

the symptoms of lumbago and lumbar movement disorder are obviously improved, the symptoms of the radioactive pain of the left lower limb are improved as compared with the front symptoms, and the treatment scheme is as before, and the lower limb is self-healed after 1 month.

The invention has the advantages that: the medicine for treating the rheumatic bone disease provided by the invention belongs to a pure traditional Chinese medicine preparation, and has the characteristics of definite curative effect, simultaneous treatment of principal and subordinate symptoms, quick effect, no toxic or side effect, portability and convenience in taking through pharmacodynamics and clinical experiment results.

Detailed Description

Example 1

The medicine comprises the following components: 6g of dragon's blood, 8g of pseudo-ginseng, 8g of peach kernel, 8g of safflower, 4g of frankincense, 4g of myrrh, 6g of diverse wormwood herb, 10g of Chinese angelica, 10g of szechuan lovage rhizome, 6g of red paeony root, 6g of white paeony root, 10g of common teasel root, 10g of eucommia bark, 10g of Chinese taxillus twig, 10g of twotooth achyranthes root, 10g of drynaria rhizome, 6g of incised notopterygium rhizome, 8g of doubleteeth pubescent angelica root, 8g of divaricate saposhnikovia root, 8g of cassia twig and 10g of common floweringquince fruit.

The preparation process comprises the following steps: respectively pulverizing 21 raw medicinal materials, sieving with a 60-mesh sieve, mixing the sieved powder according to a proportion to prepare oral medicinal powder, and mixing yellow wine and the oral medicinal powder according to a weight ratio of 0.5:1, preparing a medicine blank, standing for 2-3 days, and then mixing the medicine blank with medical vaseline according to a weight ratio of 1: mixing at a ratio of 1, and making into topical preparation.

Example 2

The medicine comprises the following components: 8g of dragon's blood, 6g of pseudo-ginseng, 6g of peach kernel, 6g of safflower, 8g of frankincense, 6g of myrrh, 4g of diverse wormwood herb, 8g of Chinese angelica, 8g of szechuan lovage rhizome, 4g of red paeony root, 4g of white paeony root, 8g of common teasel root, 8g of eucommia bark, 8g of Chinese taxillus twig, 8g of twotooth achyranthes root, 8g of drynaria rhizome, 8g of incised notopterygium rhizome, 6g of pubescent angelica root, 6g of divaricate saposhnikovia root, 6g of cassia twig and 8g of common floweringquince fruit.

Example 3

The medicine comprises the following components: 4g of dragon's blood, 5g of pseudo-ginseng, 4g of peach kernel, 4g of safflower, 6g of frankincense, 8g of myrrh, 8g of diverse wormwood herb, 6g of Chinese angelica, 6g of szechuan lovage rhizome, 8g of red paeony root, 8g of white paeony root, 6g of common teasel root, 6g of eucommia bark, 6g of Chinese taxillus twig, 6g of twotooth achyranthes root, 6g of drynaria rhizome, 10g of incised notopterygium rhizome, 10g of doubleteeth pubescent angelica root, 10g of divaricate saposhnikovia root, 10g of cassia twig and 6g of common floweringquince fruit.

Claims

1. The medicine for treating the rheumatic bone disease is characterized by being prepared from the following raw material medicines in parts by weight: 1-10g of dragon's blood, 1-10g of pseudo-ginseng, 1-10g of peach kernel, 1-10g of safflower, 1-10g of frankincense, 1-10g of myrrh, 1-10g of diverse wormwood herb, 2-12g of Chinese angelica, 2-12g of szechuan lovage rhizome, 1-10g of red paeony root, 1-10g of white paeony root, 2-12g of Chinese teasel root, 2-12g of eucommia bark, 2-12g of Chinese taxillus twig, 2-12g of twotooth achyranthes root, 2-12g of drynaria rhizome, 1-10g of notopterygium root, 1-10g of pubescent angelica root, 1-10g of divaricate saposhnikovia root, 1-10g of cassia twig and 2-12g of papaya.

2. The medicine for treating rheumatic bone diseases according to claim 1, wherein the weight ratio of each component is: 6g of dragon's blood, 8g of pseudo-ginseng, 8g of peach kernel, 8g of safflower, 4g of frankincense, 4g of myrrh, 6g of diverse wormwood herb, 10g of Chinese angelica, 10g of szechuan lovage rhizome, 6g of red paeony root, 6g of white paeony root, 10g of common teasel root, 10g of eucommia bark, 10g of Chinese taxillus twig, 10g of twotooth achyranthes root, 10g of drynaria rhizome, 6g of incised notopterygium rhizome, 8g of doubleteeth pubescent angelica root, 8g of divaricate saposhnikovia root, 8g of cassia twig and 10g of common floweringquince fruit.

3. A method for preparing a medicament for treating rheumatoid bone diseases according to claim 1, wherein: pulverizing 21 raw materials respectively, sieving with 60 mesh sieve, and mixing the sieved powders according to the proportion to obtain oral medicinal powder.

4. A method for preparing a medicament for treating rheumatoid bone diseases according to claim 1, wherein: pulverizing 21 raw materials respectively, sieving with 60 mesh sieve, mixing the sieved powder according to proportion to obtain oral medicinal powder, mixing yellow wine with oral medicinal powder to obtain medicinal blank, standing for a period of time, and mixing medicinal blank with medical vaseline to obtain topical medicine.

5. The method for preparing the medicine for treating the rheumatic bone disease according to claim 4, wherein: the weight ratio of the yellow wine to the oral medicine powder is 0.5:1.

6. the method for preparing the medicine for treating the rheumatic bone disease according to claim 4, wherein: the medicine blank is placed for 2-3 days.

7. The method for preparing the medicine for treating the rheumatic bone disease according to claim 4, wherein: the weight ratio of the medicine blank to the medical Vaseline is 1:1.

8. the method for preparing the medicine for treating the rheumatic bone disease according to claim 4, wherein: respectively pulverizing 21 raw medicinal materials, sieving with a 60-mesh sieve, mixing the sieved powder according to a proportion to prepare oral medicinal powder, and mixing yellow wine and the oral medicinal powder according to a weight ratio of 0.5:1, preparing a medicine blank, standing for 2-3 days, and then mixing the medicine blank with medical vaseline according to a weight ratio of 1: mixing at a ratio of 1, and making into topical preparation.