CN115989867B - Citrus fruit powder composition for losing weight and preparation method and application thereof - Google Patents
Citrus fruit powder composition for losing weight and preparation method and application thereof Download PDFInfo
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
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Abstract
The invention discloses a citrus fruit powder composition for losing weight, and a preparation method and application thereof, wherein the citrus fruit powder composition comprises the following components in parts by weight: comprises 10-40 parts of enzymolysis orange powder, 30-70 parts of orange fiber and 10-20 parts of guarana extract. According to the invention, the enzymolysis citrus powder, the citrus fiber and the guarana extract are compounded, so that the purposes of effectively reducing energy intake, improving fat combustion and inhibiting fat absorption are achieved. The invention also provides a preparation method of the citrus fruit powder composition, and the enzymatic hydrolysis citrus powder is prepared in an enzymatic hydrolysis mode, and contains a large amount of polyphenol substances which are beneficial to fat burning and weight losing.
Description
Technical Field
The invention relates to the field of foods, in particular to a citrus fruit powder composition, and a preparation method and application thereof.
Background
Obesity is a condition in which energy intake exceeds energy expenditure and thus fat accumulation in the body is excessive, and the World Health Organization (WHO) has formally defined obesity as a disease which is hidden upstream of the disease and is the source of the disease, and many conditions are associated with it. There are 6 hundred million people overweight and obesity according to the latest data of report on nutrition and chronic disease status of Chinese residents (2020), and currently, more than 1/2 of people overweight or obesity exist in Chinese adults, and the overweight rate of adult residents (more than or equal to 18 years) is 34.3% and the obesity rate is 16.4%. This is the first number that appears in excess of 1/2 in national survey reports. At the same time, 1/5 (19%) of children aged 6-17 and adolescents, and 1/10 (10.4%) of children under 6 years are overweight or obese. In short, the overweight and obesity problems in China are not only related to the health problems of individuals, but also a social problem, and have gradually penetrated all the layers and groups of society.
Inadequate awareness of the severity of obesity problems is the greatest threat to obesity problems. The weight-reducing medicines currently on the market can be roughly divided into the following: 1. an appetite suppressant; 2. enhancing or promoting metabolism, and accelerating lipolysis; 3. biguanides; 4. an absorption inhibitor; 5. diuretics. Although the weight-losing effect of the weight-losing medicine is obvious, the weight-losing medicine has the defects of large side effect and strong medicine dependence. While the weight-losing health-care food has no side effect and dependency, the weight-losing effect is not obvious.
In view of the foregoing, it is necessary to provide a solution to the above-mentioned problems.
Disclosure of Invention
In order to overcome the defects in the prior art, one of the purposes of the invention is as follows: the citrus fruit powder composition is prepared by mixing the enzymatic hydrolysis citrus powder, the citrus fiber and the guarana extract, integrates the physical and chemical functions of the three components, has a synergistic effect, achieves the weight-losing effect, has no side effect, and solves the existing obesity problem.
The second object of the present invention is: a method for preparing a citrus fruit powder composition is provided.
The third object of the present invention is to: provides an application of a citrus fruit powder composition in at least one of solid beverage, tablet sugar, liquid beverage, meal replacement powder, oral liquid, capsule and tablet.
One of the purposes of the invention is realized by adopting the following technical scheme:
The citrus fruit powder composition comprises the following components in parts by weight: 10-40 parts of enzymolysis orange powder, 30-70 parts of orange fiber and 10-20 parts of guarana extract.
Further, the enzymatic hydrolysis citrus powder is prepared from peel-bearing citrus fruits as raw materials.
Further, the enzymatic hydrolysis citrus powder is obtained by enzymatic hydrolysis using a combination of one or more of cellulase, naringinase and hesperidinase.
Further, the total dietary fiber content of the citrus fiber is 60% -95%.
Further, the content of caffeine in the guarana extract is greater than or equal to 10%.
The second purpose of the invention is realized by adopting the following technical scheme:
a preparation method of a citrus fruit powder composition comprises the following preparation steps:
mixing citrus fruits with enzymes for enzymolysis reaction to obtain enzymolysis liquid, filtering to obtain filtrate, concentrating, and drying to obtain enzymolysis citrus powder;
and mixing the obtained enzymatic hydrolysis orange powder with the orange fiber and the guarana extract according to the component ratio to obtain the orange fruit powder composition.
Further, the mass ratio of the citrus fruit to the enzyme is (500-1000): 1-4; the enzymolysis reaction is carried out in buffer solution with pH value of 4-6, the reaction temperature of the enzymolysis reaction is 40-60 ℃ and the reaction time is 1-5 h.
Further, the mass ratio of the citrus fruits to the buffer solution is 1 (10-40); the buffer solution is a sodium citrate system buffer solution.
Further, the citrus fiber is prepared by a preparation method comprising the following steps: extracting peeled citrus fruits in a solvent at 70-90 ℃, filtering and separating, washing filter residues, and drying to obtain the citrus fiber.
Use of a citrus fruit powder composition for reducing weight for the preparation of a weight and fat reducing product.
Further, the citrus fruit powder composition for losing weight is applied in the form of at least one of solid beverage, pressed sugar, liquid beverage, meal replacement powder, oral liquid, capsule and tablet.
Compared with the prior art, the invention has the beneficial effects that:
1. The citrus fruit powder composition of the invention comprises three substances with different benefits, namely enzymatic citrus powder, citrus fiber and guarana extract. Their respective effects are as follows: the citrus fruits have rich citrus polyphenols, dietary fibers and citrus flavone substances, have certain effects of resisting oxidation, resisting inflammation and reducing fat, and after enzymolysis, the bioavailability is improved, so that fat is easier to absorb. The citrus fiber is rich in dietary fiber, has strong oil absorption property, can absorb fat in intestinal tracts, directly prevent external fat absorption, and has the effects of regulating intestinal tracts and relaxing bowel. Guarana extract has the effects of accelerating metabolism and promoting fatty acid oxidation. The combination of the three can more effectively and scientifically control the weight, has the weight-losing effect, and has no side effect after long-term administration.
2. In the preparation method of the citrus fruit powder composition, the citrus extract is obtained in an enzymolysis mode. The amount of polyphenol extracted is more than that of the common extraction method by enzymolysis; the polyphenol substances in the enzymolysis extraction method are stronger than the polyphenol substances in the common extraction method in terms of bioavailability.
3. The citrus fruit powder composition of the present invention involves a number of aspects in the product application, allowing multiple choices for consumers and also facilitating product yield and benefit conversion.
Detailed Description
The present invention will be further described with reference to the following specific embodiments, and it should be noted that, on the premise of no conflict, new embodiments may be formed by any combination of the embodiments or technical features described below.
The following are specific examples of the present invention, in which raw materials, equipment, etc. used are available in a purchase manner except for specific limitations.
In a first aspect of the invention, there is provided a citrus fruit powder composition comprising the following components in parts by weight: 10-40 parts of enzymolysis orange powder, 30-70 parts of orange fiber and 10-20 parts of guarana extract.
The citrus fruits are rich in citrus polyphenols, dietary fibers, citrus flavones and other substances, and have certain effects of resisting oxidation, resisting inflammation and reducing fat. After enzymolysis, the bioavailability is improved and the absorption is easier. The citrus fiber is rich in dietary fiber, has strong oil absorption property, can absorb fat in intestinal tracts, directly prevent the absorption of external fat, and has the effects of regulating intestinal tracts and relaxing bowel. Guarana extract has the effects of accelerating metabolism and promoting fatty acid oxidation. The three combinations can effectively control the weight, have the function of losing weight, and have no side effect after long-term administration.
Specific types of citrus fruit include oranges, tangerines, grapefruits, oranges, and lemons. Wherein, the oranges are various, such as the common kumquats, xinjin oranges, wenzhou oranges, sichuan oranges and the like, and peel is easy to peel and is not easy to store.
The citrus fruit is large, nearly spherical, peel yellow, orange red or orange yellow. The shaddock has the largest fruit, and has three kinds of shaddock with white heart, shaddock with red heart and Shatian shaddock. The orange is oblong or round, has smooth peel and is not easy to peel. Lemon is oval or elliptic, and has yellow peel and heavy sour taste. Preferably, the citrus fruit is grapefruit.
In one embodiment, the enzymatic citrus powder is prepared from peel-bearing citrus fruit as a starting material.
In one embodiment, the enzymatic citrus powder is obtained by enzymatic hydrolysis using a combination of one or more of cellulase, naringinase, and hesperidinase.
Cellulase is a generic term for a group of enzymes which degrade cellulose to form glucose, is not a monomeric enzyme, but a multicomponent enzyme system with a synergistic effect, is a compound enzyme, mainly composed of exo-beta-glucanase, endo-beta-glucanase, beta-glucosidase and the like, and also has xylanase with very high activity, acts on cellulose and products derived from cellulose. The microbial cellulase converts insoluble cellulose into glucose, so that the cell wall of the citrus fruit cells is decomposed, and the required cell core material is obtained.
In one embodiment, the citrus fiber has a total dietary fiber content of 60% to 95%.
The enzymatic citrus powder itself also contains some amount of soluble dietary fiber, but the amount produced is far from sufficient to meet the product's own needs and therefore needs to be met by additional citrus fiber extraction.
In one embodiment, the guarana extract has a caffeine content of greater than or equal to 10%.
The caffeine has the function of improving basic metabolic capacity, thereby being beneficial to achieving the purposes of losing weight and reducing fat.
In a second aspect of the present invention, there is provided a method for preparing a citrus fruit powder composition, comprising the steps of subjecting citrus fruit to an enzymatic hydrolysis reaction with an enzyme to obtain an enzymatic hydrolysate, filtering the enzymatic hydrolysate to obtain a filtrate, concentrating the filtrate, and drying the filtrate to obtain an enzymatic citrus powder;
and mixing the obtained enzymatic hydrolysis orange powder with the orange fiber and the guarana extract according to the component ratio to obtain the orange fruit powder composition.
In one embodiment, before adding the enzyme, buffer solution is used for adjusting the pH value of the solution containing the orange powder to 4-6, and the enzyme is added after heating to 40-60 ℃; the reaction temperature of the enzymolysis reaction is 40-60 ℃ and the reaction time is 1-5 h.
In one embodiment, the mass ratio of citrus fruit to enzyme is (500-1000): 1-4; the mass ratio of the citrus fruits to the buffer solution is 1 (10-40); the buffer solution is a sodium citrate system buffer solution.
In one embodiment, the citrus fiber is prepared by a process comprising the steps of: extracting peeled citrus fruits in a solvent at 70-90 ℃, filtering and separating, washing filter residues, and drying to obtain the citrus fiber; preferably, the solution is water.
In a third aspect of the invention, the citrus fruit powder composition is used to prepare a fat-reducing product.
In one embodiment, the citrus fruit powder composition for reducing weight is prepared by at least one of solid beverage, pressed sugar, liquid beverage, meal replacement powder, oral liquid, capsule and tablet.
The citrus fruit powder composition and the preparation method thereof according to the present invention are described below with reference to specific examples.
Example 1
A citrus fruit powder composition comprises 40 parts of enzymolysis citrus powder, 53 parts of citrus fiber and 10 parts of guarana extract.
The preparation method of the composition comprises the following steps: the citrus fruit powder composition is obtained by mixing 600 g of the enzymatic citrus powder, 800 g of the citrus fiber and 150 g of the guarana extract.
The preparation method of the enzymatic hydrolysis orange powder comprises the following steps: slicing young fruits in shaddock, drying, pulverizing, adding 2kg of shaddock powder, adding 80L of water, adjusting pH to 4 with citric acid, dodecanol and sodium phosphate, heating to 40deg.C, and adding 4mL of cellulase. And (3) after enzymolysis and extraction for 1h, heating to 85-90 ℃ and inactivating enzymes for 15 minutes, filtering, collecting filtrate, concentrating, and spray-drying to obtain the enzymolysis citrus powder.
The preparation method of the citrus fiber comprises the following steps: crushing peeled citrus fruits, adding a solvent to extract at 70-90 ℃, filtering and separating, taking filter residues, washing and drying to obtain the citrus fibers.
The citrus fruit powder composition is applied to prepare pressed candy, and the preparation method comprises the following steps: mixing the citrus fruit powder composition with 150 g of microcrystalline cellulose, granulating, and tabletting to obtain 1 g/tablet of enzymolysis citrus tabletting candy.
Example 2
A citrus fruit powder composition comprises 40 parts of enzymolysis citrus powder, 53 parts of citrus fiber and 10 parts of guarana extract.
The preparation method of the composition comprises the following steps: the citrus fruit powder composition is obtained by mixing 600 g of the enzymatic citrus powder, 800 g of the citrus fiber and 150 g of the guarana extract.
The preparation method of the enzymatic hydrolysis orange powder comprises the following steps: slicing young fruits in shaddock, drying, pulverizing, adding 2kg of shaddock powder, adding 80L of water, adjusting pH to 6 with citric acid, dodecanol and sodium phosphate, heating to 60deg.C, and adding 4mL of cellulase. And (3) after enzymolysis and extraction for 5 hours, heating to 85-90 ℃ to inactivate enzymes for 15 minutes, filtering, collecting filtrate, concentrating, and spray-drying to obtain the enzymolysis citrus powder.
The preparation method of the citrus fiber comprises the following steps: crushing peeled citrus fruits, adding a solvent to extract at 70-90 ℃, filtering and separating, taking filter residues, washing and drying to obtain the citrus fibers.
The citrus fruit powder composition is applied to prepare pressed candy, and the preparation method comprises the following steps: mixing the citrus fruit powder composition with 150 g of microcrystalline cellulose, granulating, and tabletting to obtain 1 g/tablet of enzymolysis citrus tabletting candy.
Example 3
A citrus fruit powder composition comprises 10 parts of enzymolysis citrus powder, 54 parts of citrus fiber and 13 parts of guarana extract.
The preparation method of the composition comprises the following steps: the citrus fruit powder composition is obtained by mixing 200 g of the enzymatic citrus powder, 1080 g of citrus fiber and 260 g of the guarana extract.
The preparation method of the enzymatic hydrolysis orange powder comprises the following steps: slicing young fruits in shaddock, drying, pulverizing, adding 2kg of shaddock powder, adding 80L of water, adjusting pH to 5 with citric acid, dodecanol and sodium phosphate, heating to 50deg.C, and adding 4mL of cellulase. And (3) after the enzymolysis and extraction are carried out for 4 hours, the temperature is raised to 85-90 ℃ and the enzyme is inactivated for 15 minutes, then the filtration is carried out, the filtrate is collected, concentrated and spray-dried, thus obtaining the enzymolysis citrus powder.
The preparation method of the citrus fiber comprises the following steps: crushing peeled citrus fruits, adding a solvent to extract at 70-90 ℃, filtering and separating, taking filter residues, washing and drying to obtain the citrus fibers.
The citrus fruit powder composition is applied to prepare pressed candy, and the preparation method comprises the following steps: mixing the citrus fruit powder composition with 150 g of microcrystalline cellulose, granulating, and tabletting to obtain 1 g/tablet of enzymolysis citrus tabletting candy.
Example 4
A citrus fruit powder composition comprises 20 parts of enzymolysis citrus powder, 30 parts of citrus fiber and 15 parts of guarana extract.
The preparation method of the composition comprises the following steps: 600 g of the enzymolysis orange powder, 900 g of the orange fiber and 450 g of the guarana extract are used for mixing to obtain the orange powder composition.
The preparation method of the enzymatic hydrolysis orange powder comprises the following steps: slicing young fruits in shaddock, drying, pulverizing, adding 2kg of shaddock powder, adding 80L of water, adjusting pH to 5 with citric acid, dodecanol and sodium phosphate, heating to 50deg.C, and adding 4mL of cellulase. And (3) after the enzymolysis and extraction are carried out for 4 hours, the temperature is raised to 85-90 ℃ and the enzyme is inactivated for 15 minutes, then the filtration is carried out, the filtrate is collected, concentrated and spray-dried, thus obtaining the enzymolysis citrus powder.
The preparation method of the citrus fiber comprises the following steps: crushing peeled citrus fruits, adding a solvent to extract at 70-90 ℃, filtering and separating, taking filter residues, washing and drying to obtain the citrus fibers.
The citrus fruit powder composition is applied to prepare pressed candy, and the preparation method comprises the following steps: mixing the citrus fruit powder composition with 150 g of microcrystalline cellulose, granulating, and tabletting to obtain 1 g/tablet of enzymolysis citrus tabletting candy.
Example 5
A citrus fruit powder composition comprises 20 parts of enzymolysis citrus powder, 70 parts of citrus fiber and 20 parts of guarana extract.
The preparation method of the composition comprises the following steps: 600 g of the enzymolysis orange powder, 2100 g of orange fiber and 600 g of guarana extract are used for mixing to obtain the orange fruit powder composition.
The preparation method of the enzymatic hydrolysis orange powder comprises the following steps: slicing young fruits in shaddock, drying, pulverizing, adding 2kg of shaddock powder, adding 80L of water, adjusting pH to 5 with citric acid, dodecanol and sodium phosphate, heating to 50deg.C, and adding 4mL of cellulase. And (3) after the enzymolysis and extraction are carried out for 4 hours, the temperature is raised to 85-90 ℃ and the enzyme is inactivated for 15 minutes, then the filtration is carried out, the filtrate is collected, concentrated and spray-dried, thus obtaining the enzymolysis citrus powder.
The preparation method of the citrus fiber comprises the following steps: crushing peeled citrus fruits, adding a solvent to extract at 70-90 ℃, filtering and separating, taking filter residues, washing and drying to obtain the citrus fibers.
The citrus fruit powder composition is applied to prepare pressed candy, and the preparation method comprises the following steps: mixing the citrus fruit powder composition with 150 g of microcrystalline cellulose, granulating, and tabletting to obtain 1 g/tablet of enzymolysis citrus tabletting candy.
Comparative example 1
A citrus fruit powder composition comprising 40 parts of citrus powder, 53 parts of citrus fiber and 10 parts of guarana extract.
The preparation method of the composition comprises the following steps: the citrus fruit powder composition was obtained by mixing 600 g of the citrus powder, 800 g of citrus fiber, and 150 g of guarana extract.
Preparing common orange powder: slicing young fruit of fructus Citri Grandis, drying, pulverizing, adding 2kg of fructus Citri Grandis powder, adding 80L of water, extracting at 80-90deg.C for 2 hr, and continuously extracting twice. Filtering, collecting filtrate, concentrating, and spray drying to obtain mandarin orange powder without enzymolysis.
The preparation method of the citrus fiber comprises the following steps: crushing peeled citrus fruits, adding a solvent to extract at 70-90 ℃, filtering and separating, taking filter residues, washing and drying to obtain the citrus fibers.
The citrus fruit powder composition is applied to prepare pressed candy, and the preparation method comprises the following steps: mixing the citrus fruit powder composition with 150 g of microcrystalline cellulose, granulating, and tabletting to obtain 1 g/tablet citrus tabletting candy.
Comparative example 2
A weight reducing composition comprises common citrus powder and microcrystalline cellulose.
The weight-reducing composition is applied to be made into pressed candy, and the preparation method comprises the following steps: 1500 g of common orange powder and 150 g of microcrystalline cellulose are mixed, granulated and tabletted to prepare the orange tabletted candy with the weight of 1 g/tablet.
Comparative example 3
A weight reducing composition comprises citrus fiber and microcrystalline cellulose.
The weight-reducing composition is applied to be made into pressed candy, and the preparation method comprises the following steps: 1500 g of citrus fiber is mixed with 150 g of microcrystalline cellulose, and the mixture is granulated and tabletted to prepare 1 g/tablet of citrus fiber tabletted candy.
Comparative example 4
A weight reducing composition comprises guarana extract and microcrystalline cellulose.
The weight-reducing composition is applied to be made into pressed candy, and the preparation method comprises the following steps: 1500 g of the guarana extract is mixed with 150 g of microcrystalline cellulose, and the mixture is granulated and tabletted to prepare 1 g/tablet of guarana tabletted candy.
In the above embodiments, the parts of the components of each material are not limited to the above parts, and the parts of the components of each material may be other parts of components described in the present invention, which are not described herein.
Performance testing
1. Subjects and methods
1.1 Subjects
All subjects in the experiment are respectively subjected to body mass index evaluation before the experiment, and the body mass index evaluation is carried out on 50 men and 50 women in 100 subjects; the ages were between 18 and 45 years, with an average age (30.7.+ -. 6.5).
1.2 Experimental methods
Grouping: 100 subjects were randomized into 10 groups, negative control group (n=10), example 1 (n=10), example 2 (n=10), example 3 (n=10), example 4 (n=10), example 5 (n=10), comparative example 1 (n=10), comparative example 2 (n=10), comparative example 3 (n=10), comparative example 4 (n=10). The negative control group took placebo, 2 granules/day; the examples and comparative examples were taken continuously for 8 weeks as described above. During the whole experiment, diet was not controlled, exercise was not deliberately increased, and other diet foods and medicines were prohibited.
1.3 Test index
1.3.1 Body composition test: the body weight, percent body fat, and body mass index of the subjects were measured using a DX200 ultra-strong body composition tester before and after the experiment.
1.4 Data statistics
The data obtained were treated with Excel and statistical analysis was performed on the test data using single blind method and t-test, expressed as mean±sd.
Experimental results and analysis
2.1 Results of body composition analysis
2.1.1 Test results of weight measurements of subjects before and after the experiment
Table 1: body weight change of subjects before and after the experiment
Note that: * Significant difference (P < 0.05) before and after the experiment, and significant difference (P < 0.05) before and after the experiment
P<0.01)
As can be seen from table 1, the weight of each of example 1, example 2, example 3, example 4, example 5 and comparative example 1 was reduced after the experiment compared with that before the experiment, wherein the example 1, example 2, example 3, example 4 and example 5 had a very significant difference (P < 0.01), the comparative example 1 had a significant difference (P < 0.05), and the comparative example 2, comparative example 3 and comparative example 4 had no significant difference, and the weight of each of the example groups was reduced more than that of the comparative example group. The weight loss was 2-5kg for the 8 week period of the example group and 1.43kg for the comparative example group. When the raw material of the enzymatic citrus powder produced under different experimental conditions was used to produce a pressed candy, the experimental results obtained by the weight difference of 4.02kg in example 1 and the weight difference of 3.43kg in example 2 were also different to some extent. The method shows that the enzymolysis orange powder prepared under different conditions has different contents of the obtained orange polyphenol, so that the effects of reducing weight and fat of the pressed candy are different; at the same dosage, the weight difference of 4.02kg in the example 1 and the weight difference of 1.43kg in the comparative example 1 show that the effect of enzymolysis of the citrus powder is more remarkable than that of common extraction of the citrus powder; finally, the effects of weight and fat reduction of the formula are obtained through the example and the comparative example 2, the comparative example 3 and the comparative example 4, wherein the effects of reducing weight and fat of the formula are achieved through the synergistic effect of the enzymolysis of citrus powder, citrus fiber and guarana extract, and the effect of single component is poor.
2.1.2 Results of the percent body fat detection before and after the experiment
Table 2: percent body fat test results of the subject
Significant difference (P < 0.05) before and after the experimental period is noted, and significant difference (P < 0.05) before and after the experimental period is noted
P<0.01)
As can be seen from table 2, the body fat percentage of each of examples 1,2, 3,4, 5 and 1,2, 3 and 4 was reduced after the experiment compared with that before the experiment, and the body fat percentage of each of examples 1,2, 3,4 and 5 was significantly different (P < 0.01), the body fat percentage of each of examples 1, 1 and 4 was significantly different (P < 0.05), and the body fat percentage of each of examples 2, 3 and 4 was reduced more than that of each of examples.
As shown by the experiments of the above examples and comparative examples, the weight-reducing composition of the present invention can accelerate metabolism of the body and increase the ability of burning heat, and can accelerate utilization of fat without controlling diet and deliberate exercise, in the experiment, the weight reduction amplitude of the examples is significantly larger than that of the negative control group, the body fat percentage is also significantly reduced, which means that the weight of the subjects takes the weight-reducing composition of the present invention and simultaneously reduces the fat, the body fat percentage is reduced, and thus the purpose of weight reduction is achieved, and the experimental results of the weight differences and the 3-5 body fat percentage differences in the examples 1, 2,3 and 4 are the experimental results of the comparative examples 1, 2,3 and 4 in sequence: 4.02kg, 3.43kg, 3.64kg, 3.32kg, 5.6kg; comparative example: 1.43kg, 0.7kg, 0.51kg, 0.11kg body weight difference and examples: 5.15, 4.39, 2.86, 3.14, 3.18; comparative example: 1.33, 0.36, 0.18, 0.21 percent body fat, also exhibit significant differences in body weight and body fat ratio, with more significant example effects, demonstrating the synergistic effect of the example formulations.
The above embodiments are only preferred embodiments of the present invention, and the scope of the present invention is not limited thereto, but any insubstantial changes and substitutions made by those skilled in the art on the basis of the present invention are intended to be within the scope of the present invention as claimed.
Claims (8)
1. A citrus composition for weight loss, which is characterized by comprising the following components in parts by weight: 10-40 parts of enzymolysis orange powder, 30-70 parts of orange fiber and 10-20 parts of guarana extract;
the enzymatic hydrolysis orange powder is prepared by taking orange fruits with peels as raw materials, wherein the orange fruits are grapefruits;
The enzymatic hydrolysis orange powder is obtained by enzymatic hydrolysis with cellulase.
2. A citrus composition for weight loss according to claim 1, wherein said citrus fiber has a total dietary fiber content of 60% to 95%;
The content of caffeine in the guarana extract is more than or equal to 10%.
3. A process for preparing a citrus composition for weight loss according to any of claims 1-2, comprising the steps of: mixing the citrus fruit with the peel with enzyme for enzymolysis reaction to obtain enzymolysis materials, filtering to obtain filtrate, concentrating, and drying to obtain enzymolysis citrus powder; and mixing the enzymatic hydrolysis orange powder with the orange fiber and the guarana extract according to a proportion to obtain the orange composition.
4. A method of producing a citrus composition for weight loss according to claim 3, wherein the mass ratio of citrus fruit to enzyme is (500-1000): 1-4; the enzymolysis reaction is carried out in a buffer solution with pH value of 4-6; the reaction temperature of the enzymolysis reaction is 40-60 ℃ and the reaction time is 1-5 h.
5. A method of preparing a citrus composition for weight loss according to claim 4, wherein the mass ratio of citrus fruit to buffer is 1 (10-40); the buffer solution is a sodium citrate system buffer solution.
6. A process for preparing a citrus composition for weight loss according to claim 5, wherein said citrus fiber is prepared by a process comprising the steps of: extracting peeled citrus fruits in a solvent at 70-90 ℃, filtering and separating, washing filter residues, and drying to obtain the citrus fiber.
7. Use of a citrus composition for weight loss according to any of claims 1-2, wherein said citrus composition is used for the preparation of a weight loss and fat reduction product.
8. Use of a citrus composition for weight loss according to claim 7, wherein the citrus composition is prepared for use in the form of at least one of a solid beverage, a tabletted sugar, a liquid beverage, meal replacement powder, an oral liquid, a capsule, a tablet.
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| CN202211563542.0A CN115989867B (en) | 2022-12-07 | 2022-12-07 | Citrus fruit powder composition for losing weight and preparation method and application thereof |
| PCT/CN2023/135748 WO2024120305A1 (en) | 2022-12-07 | 2023-12-01 | Citrus composition for weight loss, and preparation method therefor and use thereof |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106579108A (en) * | 2016-11-28 | 2017-04-26 | 钟山县洲星食品有限公司 | Composition containing orange powder and production method of composition |
| CN107048365A (en) * | 2017-04-20 | 2017-08-18 | 北京市永康年健康科技有限责任公司 | A kind of citrus guarana product for being used to lose weight and its preparation method and application |
| CN108771081A (en) * | 2018-06-25 | 2018-11-09 | 天津倍菲睿德生物科技有限公司 | Haematococcus pluvialis citrus fruit fibres compound solid beverage of weight-reducing and preparation method thereof |
| CN113017089A (en) * | 2021-04-08 | 2021-06-25 | 上海菁睿健康管理有限公司 | Meal replacement composition for improving basal metabolism level and preparation method and application thereof |
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| CN105725191A (en) * | 2016-02-16 | 2016-07-06 | 江苏隆力奇生物科技股份有限公司 | Composition having face-beautifying and weight-losing effects and preparation method and application thereof |
| CN107319509B (en) * | 2017-05-22 | 2020-07-14 | 河北兄弟伊兰食品科技股份有限公司 | Method for continuously preparing pectin and fiber from fruits |
| CN108477455B (en) * | 2018-03-16 | 2022-02-18 | 湖南省农产品加工研究所 | Preparation method of whole citrus fruit superfine powder and whole citrus fruit superfine powder |
| CN115989867B (en) * | 2022-12-07 | 2024-05-28 | 广东金骏康生物技术有限公司 | Citrus fruit powder composition for losing weight and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106579108A (en) * | 2016-11-28 | 2017-04-26 | 钟山县洲星食品有限公司 | Composition containing orange powder and production method of composition |
| CN107048365A (en) * | 2017-04-20 | 2017-08-18 | 北京市永康年健康科技有限责任公司 | A kind of citrus guarana product for being used to lose weight and its preparation method and application |
| CN108771081A (en) * | 2018-06-25 | 2018-11-09 | 天津倍菲睿德生物科技有限公司 | Haematococcus pluvialis citrus fruit fibres compound solid beverage of weight-reducing and preparation method thereof |
| CN113017089A (en) * | 2021-04-08 | 2021-06-25 | 上海菁睿健康管理有限公司 | Meal replacement composition for improving basal metabolism level and preparation method and application thereof |
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