CN115895856A - Sample introduction system for molecular detection card - Google Patents

Abstract

The invention provides a molecular detection card sample introduction system which comprises a transport ship, a sample frame, a sample tube, a molecular detection card, a vacuum cavity and a heat gun, wherein the sample tube and the molecular detection card are arranged in the sample frame, the sample frame is arranged in the transport ship, the transport ship transports the sample frame to the vacuum cavity, the molecular detection card comprises a first liquid suction port, a second liquid suction port, a third liquid suction port and a molecular detection card cavity, the first liquid suction port, the second liquid suction port and the third liquid suction port are respectively connected with the molecular detection card cavity, the molecular detection card further comprises a non-deformable card body, the molecular detection card cavity is arranged in the non-deformable card body, and the heat gun can move to the first liquid suction port, the second liquid suction port and the third liquid suction port. The technical problem of pollution in the sample feeding process is solved, and meanwhile, full automation can be realized.

Description

Sample introduction system for molecular detection card

Technical Field

The invention relates to the field of in-vitro diagnostic molecular detection instruments, in particular to a molecular detection card sample introduction system.

Background

The present in vitro diagnosis molecular detector is mainly used for molecular diagnosis, which is a technology for detecting the change of the structure or expression level of genetic materials in a patient body by applying a molecular biology method to make diagnosis, the molecular diagnosis is a main method for predicting diagnosis, which can not only carry out diagnosis of individual genetic diseases, but also carry out prenatal diagnosis, and the molecular diagnosis mainly refers to the detection of various structural proteins, enzymes, antigen antibodies and immune active molecular genes related to the diseases.

In view of the frequent replacement of the molecular detection plate of the in vitro diagnostic molecular detector, the use frequency is high, and the sealing requirement in the in vitro diagnostic molecular detector is relatively high.

The existing automatic large-flux analysis instrument can not work in the common laboratory environment because of the pollution control problem, and can be used in the common laboratory environment only by solving the pollution problem.

The sample introduction process increases a small risk for pollution, so a new sample introduction system of the molecular detection card is to be provided to solve the pollution problem and realize automation.

Disclosure of Invention

The invention provides a sample introduction system of a molecular detection card, which at least solves the technical problem of pollution in the sample introduction process in the prior art.

The invention provides a sample introduction system of a molecular detection card, which comprises a transport ship, a sample rack, a sample tube, the molecular detection card, a vacuum cavity and a heat gun, wherein the sample tube and the molecular detection card are arranged in the sample rack, the sample rack is arranged in the transport ship, the transport ship transports the sample rack to the vacuum cavity, the molecular detection card comprises a first liquid suction port, a second liquid suction port, a third liquid suction port and a molecular detection card cavity, the first liquid suction port, the second liquid suction port and the third liquid suction port are respectively connected with the molecular detection card cavity, the molecular detection card further comprises an undeformable card body, the molecular detection card cavity is arranged in the undeformable card body, and the heat gun can move to the first liquid suction port, the second liquid suction port and the third liquid suction port.

Optionally, after the sample to be detected, which needs to be detected by the molecular detection card, is loaded into the first liquid suction port and the second liquid suction port, the transport ship transports the sample rack to the vacuum chamber, the vacuum chamber and the transport ship form a vacuum chamber, and the first liquid suction port and the second liquid suction port containing the sample to be detected are evacuated to a negative pressure.

Optionally, the heat gun includes a first heat gun and a second heat gun, the first heat gun is movable to the third liquid suction port to heat-seal the third liquid suction port, and the second heat gun is movable to the first liquid suction port and the second liquid suction port to heat-seal the first liquid suction port and the second liquid suction port.

Optionally, a spout-shaped avoiding structure is arranged on the sample tube.

Optionally, the number of the sample tubes and the number of the molecular detection cards in the sample rack are respectively greater than one.

Optionally, the cavity of the molecular detection card includes a liquid storage cavity, a mixing cavity, a first amplification cavity and a second amplification cavity, the first liquid suction port is communicated with the first amplification cavity, the second liquid suction port is communicated with the liquid storage cavity, the liquid storage cavity is connected with the mixing cavity, the mixing cavity is connected with the first amplification cavity, the mixing cavity is connected with the second amplification cavity, and the third liquid suction port is communicated with the second amplification cavity; and/or

In an initial state, the liquid storage chamber is connected but not communicated with the mixing chamber, the mixing chamber is connected but not communicated with the first amplification chamber, and the mixing chamber is connected and communicated with the second amplification chamber; and/or

In the working state, the liquid storage cavity is communicated with the mixing cavity, the mixing cavity is communicated with the first amplification cavity, and the mixing cavity is communicated with the second amplification cavity.

Optionally, the liquid storage chamber and/or the mixing chamber and/or the first amplification chamber are deformable chambers.

Optionally, a pressing shell is disposed on the non-deformable card body, the pressing shell is disposed outside the liquid storage cavity and/or the first amplification cavity, and the pressing shell is forced to act on the liquid storage cavity and/or the first amplification cavity to deform the liquid storage cavity and/or the first amplification cavity; and/or

The number of the mixing cavities is more than 1, and the mixing cavities with the number more than 1 are sequentially connected in series.

Optionally, the rims of the liquid storage chamber, the mixing chamber, the first amplification chamber, and the second amplification chamber are not deformable; and/or

The liquid storage chamber is connected to the mixing chamber through a first reaction liquid valve V1, the mixing chamber is connected to the first amplification chamber through a second amplification valve V2, and the mixing chamber is connected to the second amplification chamber through a third valve V3.

Optionally, the sample injection system of the molecular detection card is a sample injection system for detecting and vacuumizing the molecular detection card in real time.

The sample introduction system of the molecular detection card provided by the invention can realize the use of a full-automatic molecular diagnostic instrument in a common laboratory environment, consumables and samples can be automatically introduced and withdrawn, machines can be cascaded in a large scale, and the simultaneous detection of a large flux and multiple varieties is realized.

The technical scheme of the invention has the beneficial effects that:

1. carrying out spacecapsule type negative pressure sample introduction;

2. the liquid inlet pipe is thoroughly sealed by welding;

3. the sample inlet pipe penetrates into the liquid level to suck samples, and pollutants in the outside air are prevented from entering;

4. the sample tube has the structure of dodging, avoids during the application of sample and the sample suction tube collision of consumptive material.

The liquid level of the sample introduction system, the spacecapsule type sample introduction system, provided by the invention, is isolated from pollutants and is completely sealed, so that the pollution problem in the amplification process is solved, and the pollutant leakage is completely avoided by adopting a mode of welding the sample introduction port; when the sample enters the reaction tube, the liquid suction head is below the liquid level, and the liquid level seals the pollution in the outside air; the negative pressure automatically sucks the sample into the molecular detection card, the TI P head does not need to be moved, and pollution sources are reduced.

Drawings

The foregoing and other objects, features and advantages of exemplary embodiments of the invention will be apparent from the following detailed description, which proceeds with reference to the accompanying drawings. Several embodiments of the invention are illustrated by way of example, and not by way of limitation, in the figures of the accompanying drawings and in which:

FIG. 1 is a schematic view of an alternative sample introduction system for a molecular test card according to an embodiment of the present invention;

FIG. 2 is a schematic view of an alternative molecular detection card in accordance with embodiments of the present invention;

FIG. 2-1 is a schematic illustration of an alternative embodiment of the invention shown in FIG. 2;

FIG. 3 is a schematic diagram of a transportation process of an alternative sample injection system for a molecular detection card according to an embodiment of the present invention;

FIG. 4 is a schematic view of an alternative embodiment of a sample introduction system for a molecular detection card;

FIG. 5 is a schematic diagram illustrating an alternative heat sealing process for a sample injection system of a molecular test card according to an embodiment of the present invention;

FIG. 6 is a schematic diagram of a sample tube of an alternative sample introduction system of a molecular detection card according to an embodiment of the present invention;

FIG. 7 is a schematic diagram of another preferred structure of an alternative molecular detection card according to an embodiment of the present invention;

FIG. 8 is a perspective view of FIG. 7;

fig. 8-1 is a schematic view of the partially exploded structure of fig. 7.

The following detailed description is intended to further illustrate but not limit the invention, the following example being only one preferred embodiment of the invention.

Detailed Description

The principles and spirit of the present invention will be described with reference to a number of exemplary embodiments. It should be understood that these embodiments are given only for the purpose of enabling those skilled in the art to better understand and to implement the present invention, and are not intended to limit the scope of the present invention in any way. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.

As shown in fig. 1, fig. 1 is a schematic diagram of a molecular detection card sample feeding system according to an embodiment of the present invention, the molecular detection card sample feeding system 3 includes a transport ship 31, a sample rack 32, a sample tube 33, a molecular detection card 34, a vacuum chamber 35, and a heat gun, the sample tube 33 and the molecular detection card 34 are disposed in the sample rack 32, the sample rack 32 is disposed in the transport ship 31, the transport ship 31 transports the sample rack 32 to the vacuum chamber 35, as shown in fig. 2, fig. 2 is a schematic diagram of a molecular detection card according to an embodiment of the present invention, the molecular detection card 34 includes a first liquid suction port 341, a second liquid suction port 342, a third liquid suction port 347, and a molecular detection card chamber, the first liquid suction port 341, the second liquid suction port 342, and the third liquid suction port 347 are respectively connected to the molecular detection card chamber, the molecular detection card 34 further includes a non-deformable card body, the molecular detection card body 348 is disposed in the upper molecular detection card chamber 348, and the vacuum detection system is capable of detecting a molecular contamination of detecting a low molecular contamination in real time, and the molecular detection system is capable of detecting a low molecular contamination by using the automatic molecular detection system.

Further, as shown in fig. 3, fig. 3 is a schematic diagram of a transportation process of a sample injection system of a molecular detection card according to an embodiment of the present invention, as shown in fig. 4, fig. 4 is a schematic diagram of a vacuum pumping of a sample injection system of a molecular detection card according to an embodiment of the present invention, after a sample to be detected by the molecular detection card is loaded into the first liquid suction port 341 and the second liquid suction port 342, the transport ship 31 transports the sample rack 32 to the vacuum chamber 35, the vacuum chamber 35 and the transport ship 31 form a vacuum chamber, and the first liquid suction port 341 and the second liquid suction port 342 containing the sample to be detected are pumped into a negative pressure.

Further, as shown in fig. 5, fig. 5 is a schematic diagram illustrating a heat sealing process of a sample injection system for molecular detection card according to an embodiment of the present invention, where the heat gun includes a first heat gun 36 and a second heat gun 37, the first heat gun 36 is movable to the third liquid suction port 347 to heat seal the third liquid suction port 347, the second heat gun 37 is movable to the first liquid suction port 341 and the second liquid suction port 342 to heat seal the first liquid suction port 341 and the second liquid suction port 342.

Further, as shown in fig. 6, fig. 6 is a schematic structural diagram of a sample tube of a sample introduction system of a molecular detection card according to an embodiment of the present invention, a pot mouth-shaped avoiding structure 331 is disposed on the sample tube 33, the pot mouth-shaped avoiding structure 331 can effectively avoid collision with a sample suction tube of a consumable molecular detection card during sample addition, and each of the liquid suction ports is a liquid suction port of a sample suction tube of the molecular detection card.

Further, as shown in fig. 1, the numbers of the sample tubes 33 and the molecular detection cards 34 placed in the sample rack 32 are respectively greater than 1, so that a simultaneous multi-item and multi-sample detection function can be effectively realized.

Further, as shown in fig. 7 and 8, fig. 7 is another preferred structural schematic diagram of a molecular detection card according to an embodiment of the present invention, wherein the molecular detection card cavity includes a liquid storage cavity 343, a mixing cavity 344, a first amplification cavity 345 and a second amplification cavity 346, the first liquid suction port 341 is communicated with the first amplification cavity 345, the second liquid suction port 342 is communicated with the liquid storage cavity 343, the liquid storage cavity 343 is communicated with the mixing cavity 344, the mixing cavity 344 is communicated with the first amplification cavity 345, the mixing cavity 344 is communicated with the second amplification cavity 346, the third liquid suction port 347 is communicated with the second amplification cavity 346, and a frame 348 of the molecular detection card is not deformable;

in an initial state, i.e. when the consumable molecular detection card is not shipped, the liquid storage chamber 343 is connected to but not communicated with the mixing chamber 344, the mixing chamber 344 is connected to but not communicated with the first amplification chamber 345, and the mixing chamber 344 is connected to and communicated with the second amplification chamber 346;

in an operating state, i.e., when the consumable molecular detection card is used, the liquid storage chamber 343 communicates with the mixing chamber 344, the mixing chamber 344 communicates with the first amplification chamber 345, and the mixing chamber 344 communicates with the second amplification chamber 346.

Further, as shown in fig. 7 and 8-1, the liquid storage chamber 343, the mixing chamber 344, and the first amplification chamber 345 are deformable chambers, that is, the liquid storage chamber 343, the mixing chamber 344, and the first amplification chamber 345 may deform to squeeze out the liquid in the chambers when receiving an external force, so that the liquid flows into the next chamber, and the external force may be generated by a hard object such as a robot arm or a baffle of the system.

Further, as shown in fig. 7, 8 and 8-1, fig. 8 is a schematic perspective view of fig. 7, fig. 8-1 is a schematic partial explosion structure of fig. 7, the non-deformable card body is provided with a first pressing shell 3431 and a second pressing shell 3451, the first pressing shell 3431 and the second pressing shell 3451 are disposed outside the liquid storage chamber 343 and the first amplification chamber 345 shown in fig. 7, and the first pressing shell 3431 and the second pressing shell 3451 are forced to act on the liquid storage chamber 343 and the first amplification chamber 345 shown in fig. 7, so as to deform the liquid storage chamber 343 and the first amplification chamber 345;

the number of the mixing chambers 344 shown in fig. 7 is greater than 1, for example, the number of the mixing chambers 344 may be equal to 8, the 8 mixing chambers 344 are connected in series in sequence, so as to achieve a sufficient mixing effect and make a sufficient preparation for the second amplification of the nested PCR, and the sticky spots may be represented by origin 349 shown in fig. 7 and 8-1.

Further, as shown in FIG. 2, the rims of the liquid storage chamber 343, the mixing chamber 344, the first amplification chamber 345, and the second amplification chamber 346 cannot be deformed; the liquid storage chamber 343 is connected to the mixing chamber 344 through a first reaction liquid valve V1, the mixing chamber 344 is connected to the first amplification chamber 345 through a second amplification valve V2, and the mixing chamber 344 is connected to the second amplification chamber 346 through a third valve V3, so as to control the direction of liquid in the chamber through the valves, which can reduce pollution and realize a fully automated real-time detection and real-time vacuum sampling system, although the cost is slightly higher.

Further, the liquid storage chamber 343, the mixing chamber 344, and the first amplification chamber 345 shown in fig. 8-1 are soft air bag chambers, because the outer contour of the molecular detection card is a hard card body as shown in fig. 8-1, which can wrap the soft air bag chambers, and the liquid storage chamber 343 and the first amplification chamber 345 of the soft air bag chambers can be deformed to generate liquid flow by pressing the first pressing shell 3431 and the second pressing shell 3451, which results in low cost and also makes it possible to automatically transport and package the molecular detection card, which is a molecular detection card for detecting multiple items of nested PCR.

The realization consumptive material that a plurality of items of ordinary PCR detected inserts the pipette in the sample tube, then with the consumptive material at the indoor evacuation of vacuum chamber, opens the UNICOM atmosphere with real empty room again after, the sample from the pipette flow to each reaction intracavity, the heat is robbed and is gone up the pipette heat-seal, has freeze-dried probe, primer, taq polymerase, 4 kinds of DNTP in the reaction intracavity. Then PCR amplification and fluorescence detection are carried out.

The consumable materials for realizing detection of multiple items of nested PCR are as follows:

inserting a sampling tube into the sample tube, transporting the consumable and the sample tube into a vacuum cavity for vacuumizing, opening the vacuum cavity to communicate with the atmosphere, allowing the sample to flow into each PCR-I reaction cavity from the pipette, allowing the PCR-II reaction liquid to flow into the liquid storage cavity, thermally sealing the pipette, arranging a freeze-dried probe, a primer, taq polymerase and 4 DNTPs in the PCR-I reaction cavity, and performing PCR amplification. After the first-step amplification is completed, a PCR-II reaction liquid valve in the consumable and a valve for the first PCR-I amplification are opened, two liquids are mixed uniformly in a mixing cavity, the two valves are closed, a third valve is opened, the mixed liquid flows into a PCR-II reaction chamber, and freeze-dried probes, nested primers, taq polymerase and 4 DNTPs are arranged in the reaction cavity. After closing the third valve, PCR amplification and fluorescence detection were performed.

The sample introduction system of the embodiment of the invention consists of a sample frame, a sample tube, a molecular detection consumable, a transportation system, a heat gun, a vacuum chamber and a vacuum pumping system. The sample tube is provided with a spout-shaped avoiding structure, the sample tube is prevented from colliding with a sample suction tube of a consumable during sample adding, a pipette on a molecular detection consumable is inserted into the sample tube, liquid is always higher than a pipette port, a transport ship transports the sample frame, the sample tube and the molecular detection consumable on the sample frame and the molecular detection consumable to a vacuum-pumping vacancy, a vacuum cavity is tightly pressed with the transport ship under the action of a motion mechanism, the vacuum system vacuumizes the cavities, after all cavities in the molecular consumable (the molecular detection card 34) are vacuumized, the third pipette port 347 of a PCR-II reaction cavity (the second amplification cavity 346) is heat-sealed by the first heat gun 36, the vacuum cavity is opened, the sample and the PCR-II reaction liquid are sucked into the corresponding cavities, the transport system transports the sample frame to a heat-sealing position, and the second heat-sealing gun 37 heat-seals the first pipette port 341 and the second pipette port 342, so that the transport pipe moves to the PCR amplification position continuously, and further molecular detection is carried out.

Claims (10)

1. A molecular detection card sample introduction system, characterized in that, molecular detection card sample introduction system (3) includes a transport ship (31), a sample rack (32), a sample tube (33), a molecular detection card (34), a vacuum chamber (35) and a heat gun, the sample tube (33) and the molecular detection card (34) are disposed in the sample rack (32), the sample rack (32) is disposed in the transport ship (31), the transport ship (31) transports the sample rack (32) to the vacuum chamber (35), the molecular detection card (34) includes a first liquid suction port (341), a second liquid suction port (342), a third liquid suction port (347) and a molecular detection card cavity, the first liquid suction port (341), the second liquid suction port (342) and the third liquid suction port (347) are respectively connected with the molecular detection card cavity, the molecular detection card (34) further includes a non-deformable card body, the molecular detection card cavity is disposed in the non-deformable card cavity, the heat gun is disposed in the second liquid suction port (341), and the second liquid suction port (347) and the heat gun is disposed in the second liquid suction port (347).

2. The sample introduction system for molecular detection cards according to claim 1, wherein after the first and second pipetting ports (341, 342) are filled with a sample to be detected by the molecular detection card, the transport ship (31) transports the sample rack (32) to the vacuum chamber (35), the vacuum chamber (35) and the transport ship (31) form a vacuum chamber, and the first and second pipetting ports (341, 342) containing the sample to be detected are evacuated to a negative pressure.

3. The molecular detection card sample introduction system according to claim 1, wherein the heat gun comprises a first heat gun (36) and a second heat gun (37), the first heat gun (36) is movable to the third liquid suction port (347) to heat-seal the third liquid suction port (347), and the second heat gun (37) is movable to the first liquid suction port (341) and the second liquid suction port (342) to heat-seal the first liquid suction port (341) and the second liquid suction port (342).

4. The sample introduction system of claim 1, wherein the sample tube (33) is provided with a spout-shaped avoiding structure.

5. The molecular detection card sample introduction system according to claim 1, wherein the number of the sample tubes (33) and the molecular detection cards (34) disposed in the sample rack (32) is greater than one.

6. The molecular detection card sample injection system according to claim 1, wherein the molecular detection card cavity comprises a liquid storage cavity (343), a mixing cavity (344), a first amplification cavity (345), and a second amplification cavity (346), the first liquid suction port (341) is communicated with the first amplification cavity (345), the second liquid suction port (342) is communicated with the liquid storage cavity (343), the liquid storage cavity (343) is connected with the mixing cavity (344), the mixing cavity (344) is connected with the first amplification cavity (345), the mixing cavity (344) is connected with the second amplification cavity (346), and the third liquid suction port (347) is communicated with the second amplification cavity (346); and/or

In an initial state, the liquid storage chamber (343) is connected to but not in communication with the mixing chamber (344), the mixing chamber (344) is connected to but not in communication with the first amplification chamber (345), and the mixing chamber (344) is connected to and in communication with the second amplification chamber (346); and/or

In the working state, the liquid storage chamber (343) is communicated with the mixing chamber (344), the mixing chamber (344) is communicated with the first amplification chamber (345), and the mixing chamber (344) is communicated with the second amplification chamber (346).

7. The molecular detection card sample inlet system according to claim 6, wherein the liquid storage chamber (343) and/or the mixing chamber (344) and/or the first amplification chamber (345) is a deformable chamber.

8. The molecular detection card sample introduction system according to claim 6, wherein a pressing shell is disposed on the non-deformable card body, the pressing shell is disposed outside the liquid storage cavity (343) and/or the first amplification cavity (345), and the pressing shell acts on the liquid storage cavity (343) and/or the first amplification cavity (345) after being stressed to deform the liquid storage cavity (343) and/or the first amplification cavity (345); and/or

The number of the mixing cavities (344) is more than 1, and the mixing cavities (344) with the number more than 1 are sequentially connected in series.

9. The molecular detection card sample introduction system of claim 6, wherein the liquid storage chamber (343), the mixing chamber (344), the first amplification chamber (345), and the second amplification chamber (346) are not deformable by their borders; and/or

The liquid storage chamber (343) is connected to the mixing chamber (344) through a first reaction liquid valve (V1), the mixing chamber (344) is connected to the first amplification chamber (345) through a second amplification valve (V2), and the mixing chamber (344) is connected to the second amplification chamber (346) through a third valve (V3).

10. The molecular detection card sample introduction system according to any one of claims 1 to 9, wherein the molecular detection card sample introduction system is a sample introduction system for real-time detection and evacuation of the molecular detection card.

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