CN214142350U - Pre-installation layer and extraction layer connecting structure of nucleic acid detection system - Google Patents
Pre-installation layer and extraction layer connecting structure of nucleic acid detection system Download PDFInfo
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- CN214142350U CN214142350U CN202022259548.1U CN202022259548U CN214142350U CN 214142350 U CN214142350 U CN 214142350U CN 202022259548 U CN202022259548 U CN 202022259548U CN 214142350 U CN214142350 U CN 214142350U
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Abstract
The utility model discloses a connecting structure of a preassembly layer and an extraction layer of a nucleic acid detection system, which comprises a preassembly layer, an extraction layer connected below the preassembly layer in a sealing and rotating way and a piston assembly pushing liquid, wherein the preassembly layer comprises a plurality of preassembly bins which are not communicated; the pre-installation layer and the extraction layer are provided with a plurality of liquid flow channels and gas flow channels matched with the liquid flow channels, and the extraction layer is selectively communicated with the liquid flow channels and the gas flow channels of the corresponding pre-installation bins through rotating the extraction layer. The pre-installation layer, the extraction layer which is connected below the pre-installation layer in a sealing and rotating mode and the piston assembly which pushes liquid enable the liquid in the pre-installation bins to be selectively communicated with the liquid flow channel and the air flow channel of one of the corresponding pre-installation bins through the rotation of the extraction container and the sealing cover, the piston assembly is pushed to enable the liquid in the appointed pre-installation bin to flow into the extraction layer, the pre-installation bins are selectively communicated with the extraction layer, the number of parts is small, the arrangement is compact, the size is small, the manufacturing is convenient, and the manufacturing cost is reduced.
Description
Technical Field
The utility model relates to a nucleic acid testing equipment technical field, in particular to nucleic acid detecting system's prepackage layer and extraction layer connection structure.
Background
Laboratory tests are currently an important means for screening and diagnosing pathogenic bacteria or virus infections, and mainly comprise culture tests, microscopic tests, immunological tests and nucleic acid tests. The microscopic examination method is low in cost, simple and convenient, but has low positive rate and is easy to miss examination; the result reliability of the culture method is high, but the period is long and the cost is high; the immunological detection is simple to operate, but the detection is easy to miss due to a window period, and false positive results are often caused due to insufficient specificity of the antigen and the antibody.
The nucleic acid detection mainly takes PCR detection as a main part, the PCR is a method for synthesizing specific DNA fragments in vitro by enzymatic synthesis, and the method comprises a cycle consisting of high-temperature denaturation, low-temperature annealing and room-temperature extension reaction, so that the target DNA can be rapidly amplified. The PCR technology is very simple, has the advantages of high sensitivity, high specificity, short detection time and the like, has permeated into various biological branch science and clinical laboratory, and has important practical application value in many scientific fields of molecular biology, medical research, life science, bioengineering, genetic engineering, disease diagnosis, forensic medicine, archaeology and the like. Meanwhile, in the development process, a series of technical platforms such as QPCR, ddPCR, RAA-PCR, LAMP-PCR and the like are derived according to the mode of polymerase chain reaction.
The application document with the application number of CN202010441656.2 discloses an integrated nucleic acid extraction and amplification detection system based on PCR amplification, wherein a bag-type nucleic acid extraction chip comprises a nucleic acid extraction cavity (1), a first cleaning liquid cavity (2), a second cleaning liquid cavity (3), a third cleaning liquid cavity (4), an elution liquid cavity (5) and a waste liquid cavity (6), which are respectively used for storing various liquids required by nucleic acid extraction, and the cavities are connected through micro-channels; the flow channel type PCR chip comprises a PCR pretreatment cavity (7), a multi-temperature-zone PCR reaction flow channel (9) and a reaction liquid collecting cavity (8); the control detector comprises a first push rod controller (10), a second push rod controller (11), a third push rod controller (12), a fourth push rod controller (13), a fifth push rod controller (14), a sixth push rod controller (15), a seventh push rod controller (16), an eighth push rod controller (17), a first controllable alternating electromagnet (18), a second controllable alternating electromagnet (19), a first temperature controller (21), a second temperature controller (22), a third temperature controller (23), a fourth temperature controller (24) and a fluorescence detector (20). The system can realize nucleic acid detection quickly and accurately, can avoid nucleic acid cross contamination during detection and reduce biological safety risks, but has the defects of obvious nucleic acid extraction cavity, a first cleaning liquid cavity, a second cleaning liquid cavity, a third cleaning liquid cavity, an elution liquid cavity and a waste liquid cavity, and the defects of multiple structural components, large equipment volume and high manufacturing cost are realized by corresponding push rod controllers respectively.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a pre-installation layer and extraction layer connection structure of nucleic acid detecting system to solve at least one of the problem that exists among the foretell prior art.
The utility model adopts the following technical scheme:
a pre-installation layer and extraction layer connecting structure of a nucleic acid detection system comprises a pre-installation layer, an extraction layer which is connected below the pre-installation layer in a sealing and rotating mode and a piston assembly for pushing liquid, wherein the pre-installation layer comprises a plurality of pre-installation bins which are not communicated; the pre-installation layer and the extraction layer are provided with a plurality of liquid flow channels and gas flow channels matched with the liquid flow channels, and the extraction layer is selectively communicated with the liquid flow channels and the gas flow channels of the corresponding pre-installation bins through rotating the extraction layer.
Further, pre-installation layer top fixedly connected with top cap is equipped with a plurality of gas pockets and a feed liquor hole on the top cap, and pre-installation layer middle part is equipped with piston channel, is equipped with the first inside gas pocket the same with gas pocket quantity and circumference are arranged on the piston channel lateral wall, and gas pocket and atmosphere intercommunication are passed through at first inside gas pocket top.
Further, the extraction layer comprises an extraction container, a sealing cover fixedly connected with the top of the extraction container and a sealing plate rotatably connected with the bottom of the extraction container.
Furthermore, a plurality of external air holes matched with the first internal air holes are formed in the inner side wall of the pre-assembly layer, a plurality of radial partition plates are fixedly connected between the first internal air holes and the external air holes, grooves communicated with the first internal air holes and the external air holes are formed in the top of each partition plate, the pre-assembly layer is divided into a plurality of non-communicated pre-assembly bins by the partition plates, a top through hole which is circumferentially arranged is formed in the bottom of each pre-assembly bin, an internal through hole matched with the top through hole is formed in the sealing cover, and the top through hole is communicated with the internal through hole to form a liquid flow channel between the pre-assembly layer and the extraction layer; a plurality of outer through holes matched with the outer air holes are formed in the edge of the sealing cover, and the outer through holes, the outer air holes, the grooves and the first inner air holes form an air flow channel between the atmosphere and the extraction layer.
Furthermore, second internal air holes with the same number as the first internal air holes are also formed in the outer side wall of the piston channel, the second internal air holes are formed in each pre-installed bin and are located on the same circumference with the first internal air holes, and the top of each second internal air hole is communicated with the corresponding pre-installed bin; the top of the sealing cover is provided with a short groove matched with the second internal air hole for use.
Further, an inner bulge is arranged on the side wall of the extraction container, a side through hole matched with the outer through hole is formed in the inner bulge, and the side through hole is communicated with the inner cavity of the waste liquid layer; and the bottom of the preassembly layer is provided with a plurality of gas exchange grooves matched with the short grooves and the inner through holes for use.
Furthermore, the pre-loading bin, the partition plate and the top through hole are all 4
The utility model has the advantages as follows:
the utility model discloses a preassemble the layer, sealed rotation connects the extraction layer of preassemble layer below and pushes the piston assembly of liquid, through rotating to draw the container and sealed lid selectively with the liquid flow channel and the air current channel intercommunication of the preassemble storehouse of extraction layer rather than one of them correspondence, the piston assembly at promotion middle part makes the interior liquid flow of appointed preassemble storehouse draw the layer, realize a plurality of preassemble storehouses selectively with draw the layer intercommunication, its part structure is few, arrange the compactness, small, the simple operation, the preparation is convenient, and the manufacturing cost is reduced.
Drawings
FIG. 1 is a schematic view of a vertical end face structure of a nucleic acid detecting system;
fig. 2 is a schematic perspective view of the middle top cover of the present invention;
FIG. 3 is a schematic perspective view of the pre-assembled layer of the present invention, shown in FIG. 1;
FIG. 4 is a schematic perspective view of the pre-assembled layer of the present invention, shown in FIG. 2;
fig. 5 is a schematic perspective view of the sealing cover of the present invention;
fig. 6 is a schematic perspective view of the extraction container of the present invention 1;
fig. 7 is a schematic perspective view of the extraction container of the present invention, fig. 2;
fig. 8 is a schematic perspective view of a sealing plate of the present invention, illustrating a structure of fig. 1;
fig. 9 is a schematic perspective view of the sealing plate of the present invention shown in fig. 2.
Shown in the figure: 1-top cover, 1001-air hole, 1002-liquid inlet hole, 1003-piston hole, 2-pre-packed layer, 2001-external air hole, 2002-groove, 2003-first internal air hole, 2004-piston channel, 2005-second internal air hole, 2006-pre-packed bin, 2007-gas exchange tank, 2008-top through hole, 2009-partition plate, 3-sealing cover, 3001-internal through hole, 3002-short groove, 3003-external through hole, 4-extraction container, 4001-internal protrusion, 4002-side through hole, 4003-liquid inlet hole, 4004-waste liquid hole, 4005-sliding column, 5-sealing plate, 5001-annular sliding groove, 5002-bottom through hole, 5003-waste liquid groove, 5004-clamping column, 5005-bottom air groove, 6-rubber plug, 7-piston assembly.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention more clearly understood, the present invention will be described in further detail with reference to the accompanying drawings and detailed description. It should be noted that the embodiments and features of the embodiments of the present application may be combined with each other without conflict.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, however, the present invention may be practiced in other ways than those specifically described herein, and therefore the scope of the present invention is not limited by the specific embodiments disclosed below.
The technical solution of the present invention will be further explained with reference to the accompanying drawings.
As shown in FIGS. 1 to 9, a connection structure of a pre-loading layer and an extraction layer of a nucleic acid detection system comprises a pre-loading layer 2, an extraction layer which is connected below the pre-loading layer 2 in a sealing and rotating manner, and a piston assembly 7 for pushing liquid, wherein the pre-loading layer 2 comprises 4 non-communicated pre-loading bins 2006, and the extraction layer comprises an extraction container 4, a sealing cover 3 which is fixedly connected with the top of the extraction container 4, and a sealing plate 5 which is connected with the bottom 4 of the extraction container in a rotating manner.
The top of the pre-installation layer 2 is fixedly connected with a top cover 1, the top cover 1 is provided with 4 air holes and 1 liquid inlet hole 1002, the middle of the pre-installation layer 2 is provided with a piston channel 2004, the outer side wall of the piston channel 2004 is provided with first inner air holes 2003 which are the same in number as the air holes 1001 and are circumferentially arranged, and the top of the first inner air holes 2003 is communicated with the atmosphere through the air holes 1001.
The inner side wall of the pre-installed layer 2 is provided with 4 external air holes 2001 matched with the first internal air holes 2003 for use, 4 radial partition plates 2009 are fixedly connected between the first internal air holes 2003 and the external air holes 2001, the top of each partition plate 2009 is provided with a groove 2002 communicated with the first internal air holes 2003 and the external air holes 2001, each pre-installed layer 2 is divided into 4 non-communicated pre-installed bins 2006 by the partition plates 2009, the bottom of each pre-installed bin 2006 is provided with 1 circumferentially arranged top through hole 2008, the sealing cover 3 is provided with an inner through hole 3001 matched with the top through hole 2008 for use, and the top through holes 2008 and the inner through holes 3001 are communicated to form a liquid flow channel between the corresponding pre-installed bins 2006 and the extraction layer; the edge of the sealing cover 3 is provided with a plurality of outer through holes 3003 matched with the outer air holes 2001 for use, and the outer through holes 3003, the outer air holes 2001, the groove 2002 and the first inner air holes 2003 form an air flow channel between the atmosphere and the extraction layer.
The outer side wall of the piston channel 2004 is also provided with second internal air holes 2005 with the same number as the first internal air holes 2003, the second internal air holes 2005 are arranged in each pre-loading bin 2006 and are positioned on the same circumference with the first internal air holes 2003, and the top of each second internal air hole 2005 is communicated with the corresponding pre-loading bin 2006; the top of the sealing cover 3 is opened with a short groove 3002 used in cooperation with the second internal air hole 2005.
An inner protrusion 4001 is arranged on the side wall of the extraction container 4, a side through hole 4002 matched with the outer through hole 3003 is formed in the inner protrusion 4001, and the side through hole 4002 is communicated with an external waste liquid layer inner cavity; the bottom of the pre-assembled layer 2 is provided with a plurality of gas exchange grooves 2007 which are used with the short grooves 3002 and the inner through holes 3001.
When the second internal air hole 2005 is communicated with the short groove 3002, the space below the piston and above the sealing cover 3 is communicated with the corresponding pre-installed bin 2006, at this time, the top through hole 2008 corresponding to the pre-installed bin 2006 is also communicated with the internal through hole 3001 to form a liquid flow channel between the corresponding pre-installed bin 2006 and the extraction layer, the external through hole 3003, the external air hole 2001, the groove 2002 and the first internal air hole 2003 form an air flow channel between the atmosphere and the extraction layer, the piston moves downwards, the pressure in the corresponding pre-installed bin 2006 is increased, the liquid in the pre-installed bin 2006 is extruded downwards to flow into the extraction layer, and the gas in the extraction layer is discharged into the atmosphere.
The working process of the utility model is as follows:
1. at the initial position, filling a first cleaning solution, a second cleaning solution and an eluent into 3 pre-assembly bins without rubber plugs, opening the rubber plugs, filling a detection sample liquid into a 4 th pre-assembly bin, wherein at the moment, the short groove and the first inner air hole are communicated with the atmosphere, and the piston rod is lifted upwards to suck air;
2. the extraction container and the sealing cover rotate clockwise for 30 degrees, at the moment, the second inner air hole is communicated with the short groove to communicate the lower part of the piston and the short groove with a pre-assembly bin filled with detection sample liquid, the top through hole is communicated with the inner through hole, the outer air hole, the groove and the first inner air hole form an air flow channel between atmosphere and the extraction layer, the piston moves downwards to extrude the detection sample liquid in the pre-assembly bin downwards to flow into the extraction layer, and gas in the extraction layer is discharged into the atmosphere.
3. The extraction container and the sealing cover rotate counterclockwise by 30 degrees together and return to the initial position, the piston rod is lifted upwards to realize air suction, and the extraction container is placed at room temperature for 10min to realize cracking to realize nucleic acid adsorption, and meanwhile, an external electromagnetic chuck is started to work to realize magnetic bead enrichment;
4. the extraction container and the sealing cover rotate clockwise by 59 degrees, at the moment, the gas exchange groove 2007 communicates the space below the piston with the extraction layer through the communicating inner through hole 3001 and the short groove 3002, the extraction layer is communicated with the external waste liquid layer, the piston moves downwards, the detection sample liquid in the extraction layer is extruded downwards to flow into the external waste liquid layer, and the gas in the waste liquid layer is discharged into the atmosphere;
5. the extraction container and the sealing cover rotate together clockwise for 46 degrees, at the moment, the short groove and the first internal air hole are communicated with the atmosphere, and the piston rod is lifted upwards to realize air suction;
6. the extraction container and the sealing cover rotate clockwise for 15 degrees, at the moment, another second internal air hole is communicated with the short groove to communicate the position below the piston and the short groove with a pre-assembly bin filled with first cleaning liquid, the through hole at the top is communicated with the internal through hole, the external air hole, the groove and the first internal air hole form an air flow channel between atmosphere and the extraction layer, the piston moves downwards, the first cleaning liquid in the pre-assembly bin is extruded downwards to flow into the extraction layer, the gas in the extraction layer is discharged into the atmosphere, the external electromagnetic chuck is closed, and the magnetic bead dissociation is realized;
7. the extraction container and the sealing cover rotate counterclockwise together for 15 degrees, at the moment, the short groove and the first internal air hole are communicated with the atmosphere, the piston rod is lifted upwards to realize air suction, and meanwhile, the external electromagnetic chuck works to realize magnetic bead enrichment and stir the inside of the extraction layer to realize cleaning;
8. the extraction container and the sealing cover rotate clockwise for 59 degrees, at the moment, the other gas exchange groove communicates the space below the piston with the extraction layer through communicating the inner through hole and the short groove, the extraction layer is communicated with the external waste liquid layer, the piston moves downwards, the first cleaning liquid in the extraction layer is extruded downwards and flows into the external waste liquid layer, the gas in the waste liquid layer is discharged into the atmosphere, the electromagnetic chuck is closed, and the liberation of magnetic beads is realized;
9. the extraction container and the sealing cover rotate clockwise for 16 degrees, at the moment, the short groove and the first internal air hole are communicated with the atmosphere, and the piston rod is lifted upwards to realize air suction;
10. the extraction container and the sealing cover rotate clockwise for 30 degrees, at the moment, another second internal air hole is communicated with the short groove to communicate the position below the piston and the short groove with a pre-assembly bin filled with second cleaning liquid, the through hole at the top is communicated with the internal through hole, the external air hole, the groove and the first internal air hole form an air flow channel between atmosphere and the extraction layer, the piston moves downwards, the second cleaning liquid in the pre-assembly bin is extruded downwards to flow into the extraction layer, the gas in the extraction layer is discharged into the atmosphere, the electromagnetic chuck is closed, and the dissociation of magnetic beads is realized;
11. the extraction container and the sealing cover rotate anticlockwise for 30 degrees together, at the moment, the short groove and the first internal air hole are communicated with the atmosphere, the piston rod is lifted upwards to realize air suction, meanwhile, the electromagnetic chuck works to realize magnetic bead enrichment, and the internal part of the extraction layer is stirred to realize cleaning;
12. the extraction container and the sealing cover rotate clockwise for 59 degrees, at the moment, the other gas exchange groove communicates the space below the piston with the extraction layer through communicating the inner through hole and the short groove, the extraction layer is communicated with the external waste liquid layer, the piston moves downwards, the first cleaning liquid in the extraction layer is extruded downwards and flows into the waste liquid layer, and the gas in the waste liquid layer is discharged into the atmosphere;
13. the extraction container and the sealing cover rotate clockwise for 32 degrees, at the moment, the short groove and the first internal air hole are communicated with the atmosphere, and the piston rod is lifted upwards to realize air suction;
14. the extraction container and the sealing cover rotate clockwise for 15 degrees, at the moment, the other second inner air hole is communicated with the short groove to communicate the lower part of the piston and the short groove with a pre-assembly bin filled with eluent, the top through hole is communicated with the inner through hole, the outer air hole, the groove and the first inner air hole form an air flow channel between atmosphere and an extraction layer, the piston moves downwards, the eluent in the pre-assembly bin is squeezed downwards to flow into the extraction layer, the air in the extraction layer is discharged into the atmosphere, the external electromagnetic chuck is closed to realize the dissociation of magnetic beads, and the magnetic beads are placed at room temperature for 5min to realize the elution of nucleic acid.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. A pre-installation layer and extraction layer connecting structure of a nucleic acid detection system is characterized by comprising a pre-installation layer, an extraction layer and a piston assembly, wherein the extraction layer is connected below the pre-installation layer in a sealing and rotating mode, the piston assembly pushes liquid in the extraction layer, and the pre-installation layer comprises a plurality of pre-installation bins which are not communicated; the pre-installation layer and the extraction layer are provided with a plurality of liquid flow channels and gas flow channels matched with the liquid flow channels, and the extraction layer is selectively communicated with the liquid flow channels and the gas flow channels of the corresponding pre-installation bins through rotating the extraction layer.
2. The connecting structure of the pre-installed layer and the extraction layer of the nucleic acid detecting system according to claim 1, wherein a top cap is fixedly connected to the top of the pre-installed layer, a plurality of air holes and a liquid inlet hole are formed in the top cap, a piston channel is formed in the middle of the pre-installed layer, first inner air holes which are the same in number as the air holes and are circumferentially arranged are formed in the outer side wall of the piston channel, and the tops of the first inner air holes are communicated with the atmosphere through the air holes.
3. The connecting structure of the pre-loading layer and the extraction layer of a nucleic acid detecting system according to claim 2, wherein the extraction layer comprises an extraction container, a sealing cap fixedly connected to the top of the extraction container, and a sealing plate rotatably connected to the bottom of the extraction container.
4. The connecting structure of the pre-installation layer and the extraction layer of the nucleic acid detecting system according to claim 3, wherein the inner side wall of the pre-installation layer is provided with a plurality of external air holes matched with the first internal air holes, a plurality of radial partition plates are fixedly connected between the first internal air holes and the external air holes, the top of each partition plate is provided with a groove communicated with the first internal air holes and the external air holes, the partition plates divide the pre-installation layer into a plurality of non-communicated pre-installation bins, the bottom of each pre-installation bin is provided with a circumferentially arranged top through hole, the sealing cover is provided with an internal through hole matched with the top through hole, and the top through hole and the internal through hole are communicated to form a liquid flow channel between the pre-installation layer and the extraction layer; a plurality of outer through holes matched with the outer air holes are formed in the edge of the sealing cover, and the outer through holes, the outer air holes, the grooves and the first inner air holes form an air flow channel between the atmosphere and the extraction layer.
5. The connecting structure of the pre-loading layer and the extraction layer of a nucleic acid detecting system according to claim 4, wherein the outer sidewall of the piston channel is further provided with a second internal air hole with the same number as the first internal air holes, the second internal air hole is provided in each pre-loading bin and is located on the same circumference as the first internal air hole, and the top of each second internal air hole is communicated with the corresponding pre-loading bin; the top of the sealing cover is provided with a short groove matched with the second internal air hole for use.
6. The connection structure of the pre-assembled layer and the extraction layer of the nucleic acid detection system according to claim 5, wherein the side wall of the extraction container is provided with an inner protrusion, the inner protrusion is provided with a side through hole matched with the outer through hole, and the side through hole is communicated with the inner cavity of the waste liquid layer; and the bottom of the preassembly layer is provided with a plurality of gas exchange grooves matched with the short grooves and the inner through holes for use.
7. The nucleic acid detecting system according to any one of claims 1 to 6, wherein the number of the pre-loading chamber, the partition plate and the top through hole is 4.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113967491A (en) * | 2021-12-01 | 2022-01-25 | 苏州含光微纳科技有限公司 | Chip liquid path control system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113967491A (en) * | 2021-12-01 | 2022-01-25 | 苏州含光微纳科技有限公司 | Chip liquid path control system |
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Address after: 450000 4th floor, building 25, Liandong u Valley Industrial Park, northwest corner, intersection of Haihang East Road and 25th Street, Zhengzhou Economic and Technological Development Zone, Henan Province Patentee after: Henan Zhongjian Shian Biotechnology Co.,Ltd. Address before: 450000 4th floor, building 25, Liandong u Valley Industrial Park, northwest corner, intersection of Haihang East Road and 25th Street, Zhengzhou Economic and Technological Development Zone, Henan Province Patentee before: Henan Zhitai Biotechnology Co.,Ltd. |
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