CN115873288B - Temperature-sensitive PCR thin-wall tube - Google Patents
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Abstract
The invention discloses a temperature-sensitive PCR thin-wall tube, belonging to the technical field of PCR thin-wall tubes; the surface of the PCR thin-wall tube is coated with a temperature sensitive coating formed by heat conducting components; wherein the thermally conductive component comprises a component a and a component b; component a comprises a thermally conductive filler; the heat conducting filler comprises at least one of graphene, modified graphene oxide, graphite, carbon nano tube, boron nitride, aluminum oxide and zinc oxide; the thermal conductivity of the PCR thin-wall tube is higher than 0.85W/m.k; the invention prepares the heat conduction component with better dispersion stability, processes the PCR thin-wall tube, and forms a temperature sensitive layer on the surface of the heat conduction component to obtain the PCR thin-wall tube with excellent heat conduction performance, waterproof and antifouling performance, coating adhesion and biological corrosion resistance.
Description
Technical Field
The invention belongs to the technical field of PCR thin-wall tubes, and particularly relates to a temperature-sensitive PCR thin-wall tube.
Background
In the experimental process of PCR, a PCR thin-wall tube is usually required, and the PCR thin-wall tube is generally made of polypropylene, so that the PCR thin-wall tube has the advantages of high heat transfer rate, good sealing performance, pollution prevention and easiness in uncovering; the tube wall of the PCR reaction tube needs to ensure the effectiveness and uniformity of the indirect heat conduction of the sample, and meanwhile, the liquid in the tube can be quickly heated to the target temperature, so that the PCR thin-wall tube needs to have better temperature sensitivity.
The prior art CN113913048A discloses a high-thermal conductivity PCR reaction tube and a preparation process thereof, and the preparation method comprises the following steps: process 1: carrying out plasma pretreatment on the PCR reaction tube to obtain a pretreated PCR reaction tube; process 2: and immersing the pretreated PCR reaction tube in a heat conducting material for surface treatment to obtain the PCR reaction tube with high heat conductivity. The heat conducting material comprises modified graphene, an organic polymer, a solvent and a chemical auxiliary agent; the modified graphene is prepared from neohesperidin modified graphene oxide; the prepared PCR reaction tube has higher heat conductivity and excellent water resistance, biological pollution resistance and wear resistance.
Disclosure of Invention
The invention aims to provide a heat conduction component with good dispersion stability, which is used for treating a PCR thin-wall tube and forming a temperature sensitive layer on the surface of the heat conduction component so as to obtain the PCR thin-wall tube with good heat conduction performance, waterproof and antifouling performance, coating adhesion and biological corrosion resistance.
The technical scheme adopted by the invention for achieving the purpose is as follows:
a temperature sensitive PCR thin-wall tube, the surface of which is coated with a temperature sensitive coating formed by heat conducting components; wherein, the liquid crystal display device comprises a liquid crystal display device,
the heat conducting component comprises a component a and a component b;
component a comprises a thermally conductive filler;
the heat conducting filler comprises at least one of graphene oxide, modified graphene oxide, graphite, carbon nano tube, boron nitride, aluminum oxide and zinc oxide;
the thermal conductivity of the PCR thin-wall tube is higher than 0.85W/m.k. The invention processes the heat conduction component on the surface of the PCR thin-wall tube to form the temperature sensitive coating, which has higher heat conductivity and can quickly raise the temperature to the target temperature according to the change of the temperature.
In one embodiment of the present invention, the thermally conductive component b comprises a matrix resin and a silane coupling agent.
Further, in an embodiment of the present invention, the matrix resin in component b comprises at least one of polypyrrole, polyacetylene, polysiloxane.
Further, in one embodiment of the present invention, the matrix resin in component b is a polysiloxane.
In one embodiment of the present invention, the weight ratio of the matrix resin to the silane coupling agent in the component b is 30-50:0.5-2.
In one embodiment of the present invention, the modified graphene oxide is modified by oxyfluoride esterification. According to the invention, the graphene oxide is modified by oxyfluoride esterification and is used as a heat conducting filler to prepare a heat conducting component, so that the dispersion stability of the heat conducting component is improved; the heat conducting component is used for processing the PCR thin-wall tube to form a layer of coating on the surface of the PCR thin-wall tube, and the coating has higher heat conductivity, namely, the change of the external temperature can be better perceived and reaches the target temperature, so that the liquid in the tube is quickly heated to the target temperature; meanwhile, the heat conduction component improves the water contact angle of the PCR thin-wall tube, so that the PCR thin-wall tube has excellent waterproof and antifouling properties; and has better coating adhesion and biological corrosion resistance.
In addition, in an embodiment of the present invention, a method for preparing modified graphene oxide is also disclosed, including:
adding graphene oxide into an aqueous solution to uniformly disperse to obtain a dispersion liquid with the concentration of 0.4-0.8 mg/mL, dispersing the dispersion liquid into N, N-dimethylformamide to obtain a graphene oxide dispersion liquid with the concentration of 0.2-0.5 mg/mL, adding oxyfluoric acid and a catalyst into the graphene oxide dispersion liquid, performing ultrasonic dispersion, reacting for 10-16 h at the temperature of 55-75 ℃, washing, removing unreacted substances and the catalyst, and drying to obtain the modified graphene oxide.
Further, in an embodiment of the present invention, the weight ratio of graphene oxide to ofloxacin is 1:4-10; specifically, 1:5-10, 1:6-10, 1:7-10, 1:8-10 and 1:9-10 are selected.
Further, in an embodiment of the present invention, the catalyst is used in an amount of 0.75 to 2.5% by weight of the graphene oxide.
In one embodiment of the invention, the weight ratio of the component a to the component b is 2-5:10-20.
Further, in one embodiment of the present invention, the weight ratio of component a to component b is 2-4:15-20.
In one embodiment of the invention, the water contact angle of the PCR thin-walled tube is higher than 115 degrees.
The beneficial effects of the invention are as follows:
according to the invention, the graphene oxide is modified by oxyfluoride esterification and is used as a heat conducting filler to prepare a heat conducting component, so that the dispersion stability of the heat conducting component is improved; the heat conducting component is used for processing the PCR thin-wall tube to form a layer of coating on the surface of the PCR thin-wall tube, and the coating has higher heat conductivity, namely, the change of the external temperature can be better perceived and reaches the target temperature, so that the liquid in the tube is quickly heated to the target temperature; meanwhile, the heat conduction component improves the water contact angle of the PCR thin-wall tube, so that the PCR thin-wall tube has excellent waterproof and antifouling properties; and has better coating adhesion and biological corrosion resistance. Therefore, the invention is a heat conduction component with better dispersion stability, which is used for treating the PCR thin-wall tube, and a temperature sensitive layer is formed on the surface of the heat conduction component to obtain the PCR thin-wall tube with excellent heat conduction performance, waterproof and antifouling performance, coating adhesion and biological corrosion resistance.
Drawings
FIG. 1 is an infrared spectrum of graphene oxide and modified graphene oxide of example 1;
FIG. 2 is the average particle diameter size of graphene oxide and modified graphene oxide in example 1;
FIG. 3 is the thermal conductivity of a PCR thin walled tube;
FIG. 4 is a water contact angle of a PCR thin walled tube;
FIG. 5 shows the rate of change of thermal conductivity of PCR thin walled tubes.
Detailed Description
For further explanation of the present invention, a solid oxide fuel cell provided by the present invention will be described in detail with reference to examples, but it should be understood that these examples are given by way of illustration of detailed embodiments and specific operation procedures based on the technical scheme of the present invention, and are only for further explanation of the features and advantages of the present invention, not limitation of the claims of the present invention, and the scope of protection of the present invention is not limited to the examples described below. It should be noted that, in the present invention, the terms "comprising," "including," or any other variation thereof, are intended to cover a non-exclusive inclusion.
In the present invention, the matrix resin used is commercially available or prepared according to the prior art; the polysiloxanes according to the invention are prepared according to the prior art; the preparation method comprises the following steps:
placing reactants octamethyl cyclotetrasiloxane, concentrated sulfuric acid and deionized water in a reaction vessel, uniformly stirring, heating for reaction, diluting a product by diethyl ether after the reaction is finished, extracting by deionized water, washing to be neutral, steaming in a rotary way, heating to remove unreacted reactants, and drying to obtain polysiloxane.
In the preparation method of the polysiloxane, the weight ratio of the octamethyl cyclotetrasiloxane to the concentrated sulfuric acid to the deionized water is 25-40:0.5-1.5:0.2-0.8.
More specifically, the method for preparing the polysiloxane used in the present invention is as follows:
placing reactants octamethyl cyclotetrasiloxane, concentrated sulfuric acid and deionized water in a reaction vessel according to a weight ratio of 25-40:0.5-1.5:0.2-0.8, uniformly stirring, heating to 50-60 ℃ for reaction for 10-18 h, diluting a product by using diethyl ether with the weight of 1-2 times that of octamethyl cyclotetrasiloxane after the reaction is finished, extracting the product by using deionized water, washing the product to be neutral by using deionized water, removing diethyl ether by rotary evaporation at 35-40 ℃, heating to 85-95 ℃ for rotary evaporation, removing unreacted reactants, and drying to obtain polysiloxane.
The preparation method of the polysiloxane used in the embodiment of the invention is as follows:
placing reactants octamethyl cyclotetrasiloxane, concentrated sulfuric acid and deionized water in a reaction vessel according to a weight ratio of 35:0.5:0.8, uniformly stirring, heating to 55 ℃ for reaction for 12 hours, diluting a product by diethyl ether with the weight which is 1.5 times that of octamethyl cyclotetrasiloxane after the reaction is finished, extracting the product by deionized water, washing the product by deionized water to be neutral, removing diethyl ether by rotary evaporation at 35 ℃, heating to 95 ℃ for rotary evaporation, removing unreacted reactants, and drying to obtain polysiloxane.
The invention also discloses a preparation method of the heat conduction component, which comprises the following steps:
component a comprises a thermally conductive filler;
component b comprises a matrix resin and a silane coupling agent; and dissolving the matrix resin in a solvent, adding a silane coupling agent, adding the component a, and performing ultrasonic dispersion for 20-50 min under the power of 200-400W to obtain the heat conduction component.
In the preparation method of the heat-conducting component, the dosage of the solvent is 3-6 times of that of the matrix resin.
The invention also provides a preferred embodiment capable of improving the dispersibility and uniformity of the heat conductive component, wherein erythritol tetraacetate is added to the component b; the addition amount of the modified epoxy resin is 1-5% of the weight of the matrix resin; the erythritol tetraacetate is added into the component b and then mixed with the heat conducting filler in the component a, so that the dispersibility and uniformity of the heat conducting component can be increased, the heat conducting filler can be better dispersed in matrix resin, and the heat conducting component with better dispersibility can be further obtained, so that the PCR thin-wall tube with excellent performance can be obtained.
The invention also provides a preparation method of the temperature-sensitive PCR thin-wall tube, which comprises the following steps:
providing a PCR thin-wall tube, sequentially ultrasonically cleaning with absolute ethyl alcohol, acetone and ultrapure water, removing impurities on the surface of the PCR thin-wall tube, drying, and performing plasma pretreatment to obtain a pretreated PCR thin-wall tube;
immersing the pretreated PCR thin-wall tube in a heat-conducting component for surface treatment, and then drying at 80-110 ℃ for 10-20 min to obtain the temperature-sensitive PCR thin-wall tube.
In the preparation method of the temperature-sensitive PCR thin-wall tube, the plasma pretreatment is also a conventional experiment method, and specific plasma experiment parameters are as follows: the argon atmosphere, the argon flow is 55-65 sccm, the ventilation time is 8-12 min, the discharge vacuum degree is 15-20 Pa, the output power is 50-100W, the discharge time is 10-15 min, and the vacuum degree is set to be 0.6-1.0 Pa.
In the preparation method of the temperature-sensitive PCR thin-wall tube, the pretreated PCR thin-wall tube is pretreated in the heat-conducting component for 10-30 min.
The experimental methods in the following examples are conventional methods unless otherwise specified. Materials, reagents and the like used in the examples described below are commercially available unless otherwise specified.
Example 1:
a method for preparing modified graphene oxide, comprising;
adding graphene oxide into deionized water for uniform dispersion to obtain a dispersion liquid with the concentration of 0.5mg/mL, dispersing the dispersion liquid into N, N-dimethylformamide to obtain a graphene oxide dispersion liquid with the concentration of 0.25mg/mL, adding oxyfluoric acid and DCC into the graphene oxide dispersion liquid, wherein the weight ratio of the graphene oxide to the oxyfluoric acid is 1:6, the DCC is 0.75% of the weight of the graphene oxide, performing ultrasonic dispersion for 15min under the power condition of room temperature and 250W, then reacting for 12h at 70 ℃, washing, removing unreacted substances and DCC, and drying to obtain the modified graphene oxide.
Example 2:
the preparation method of the heat conduction component comprises the following steps:
component a comprises only the modified graphene oxide of example 1;
component b comprises polysiloxane and a silane coupling agent KH-570; 4 parts by weight of polysiloxane is dissolved in 35 parts by weight of n-hexane, 0.15 part by weight of silane coupling agent KH-570 is added, and then component a is added, wherein the weight ratio of component a to component b is 3:16, and the heat conduction component is obtained by ultrasonic dispersion for 30min under 400W power.
Example 3:
a method of preparing a thermally conductive component, differing from example 2 in that: component a comprises graphene oxide only.
Example 4:
a method of preparing a thermally conductive component, differing from example 2 in that: component a comprises only boron nitride.
Example 5:
a method of preparing a thermally conductive component, differing from example 2 in that: the weight ratio of the component a to the component b is 4:15.
Example 6:
a method of preparing a thermally conductive component, differing from example 2 in that: to component b, erythritol tetraacetate was added in an amount of 1% by weight based on the weight of the matrix resin.
Example 7:
a method of preparing a thermally conductive component, differing from example 2 in that: to component b, erythritol tetraacetate was added in an amount of 3% by weight based on the weight of the matrix resin.
Example 8:
a method of preparing a thermally conductive component, differing from example 2 in that: to component b, erythritol tetraacetate was added in an amount of 5% by weight based on the weight of the matrix resin.
Example 9:
a method of preparing a thermally conductive component, differing from example 3 in that: to component b, erythritol tetraacetate was added in an amount of 3% by weight based on the weight of the matrix resin.
Example 10:
a preparation method of a temperature-sensitive PCR thin-wall tube comprises the following steps:
providing a PCR thin-wall tube (commercially available polypropylene material), sequentially ultrasonically cleaning with absolute ethyl alcohol, acetone and ultrapure water for 15min, removing impurities on the surface of the PCR thin-wall tube, drying, and performing plasma pretreatment, wherein the plasma experimental parameters are as follows: argon atmosphere, argon flow of 60sccm, ventilation time of 10min, discharge vacuum degree of 18Pa, output power of 60W, discharge time of 12min, and vacuum degree of 0.8Pa, so as to obtain a pretreated PCR thin-walled tube;
immersing the pretreated PCR thin-wall tube in the heat-conducting component in the embodiment 2 for 15min, and then drying at 100 ℃ for 15min to obtain the temperature-sensitive PCR thin-wall tube.
Example 11:
the preparation method of the temperature-sensitive PCR thin-wall tube is different from example 9 in that: the thermally conductive component of example 2 was replaced with the thermally conductive component of example 3.
Example 12:
the preparation method of the temperature-sensitive PCR thin-wall tube is different from example 9 in that: the thermally conductive component of example 2 was replaced with the thermally conductive component of example 4.
Example 13:
the preparation method of the temperature-sensitive PCR thin-wall tube is different from example 9 in that: the thermally conductive component of example 2 was replaced with the thermally conductive component of example 5.
Example 14:
the preparation method of the temperature-sensitive PCR thin-wall tube is different from example 9 in that: the thermally conductive component of example 2 was replaced with the thermally conductive component of example 6.
Example 15:
the preparation method of the temperature-sensitive PCR thin-wall tube is different from example 9 in that: the thermally conductive component of example 2 was replaced with the thermally conductive component of example 7.
Example 16:
the preparation method of the temperature-sensitive PCR thin-wall tube is different from example 9 in that: the thermally conductive component of example 2 was replaced with the thermally conductive component of example 8.
Example 17:
the preparation method of the temperature-sensitive PCR thin-wall tube is different from example 9 in that: the thermally conductive component of example 2 was replaced with the thermally conductive component of example 9.
[ Infrared Spectrometry characterization ]
Infrared characterization of graphene oxide and modified graphene oxide with Fourier transform infrared spectrometer (model: FTIR-440), tabletting with potassium bromide, and scanning at 500-4000cm -1 。
Fig. 1 is an infrared spectrum of graphene oxide and modified graphene oxide in example 1. As can be seen from fig. 1, curves a and b are graphene oxide and modified graphene oxide, respectively; compared with graphene oxide, 3030cm of modified graphene oxide -1 The characteristic absorption peak appearing nearby is the flexible vibration of benzene ring; at 1750cm -1 The characteristic absorption peak appearing nearby is the stretching vibration of the ester group; in addition, at 1315cm -1 Stronger C-F characteristic absorption peaks appear nearby; therefore, the modified graphene oxide is successfully prepared by adopting the oxyfluoride to esterify the modified graphene oxide.
[ particle size measurement ]
Determining the particle size of the particles according to the movement speed of the particles in the liquid by utilizing a dynamic light scattering and photon correlation spectroscopy technology; the test samples (graphene oxide and modified graphene oxide in example 1) were dispersed in a liquid uniformly to obtain a test dispersion having a concentration of 0.15mg/mL, and then the average particle size of the test samples was measured.
Fig. 2 is the average particle diameter size of graphene oxide and modified graphene oxide in example 1. As can be seen from fig. 2, the average particle size of the modified graphene oxide is below 160nm, which is much lower than that of graphene oxide; the modified graphene oxide is successfully prepared by adopting the oxyfluoride to esterify the modified graphene oxide, so that the particle size of the modified graphene oxide is reduced, and the modified graphene oxide is prevented from agglomerating, so that the modified graphene oxide has excellent dispersion performance.
[ test for stability of Heat-conducting component dispersion ]
The prepared heat conductive component was allowed to stand at room temperature for 4 weeks, and whether or not precipitation occurred was observed.
TABLE 1 dispersion stability of thermally conductive Components
| Sample preparation | Phenomenon (1) |
| Example 2 | A small amount of precipitate was generated at 3 weeks |
| Example 3 | More precipitate appeared at 5d |
| Example 4 | More precipitate appeared at 1 week |
| Example 5 | A small amount of precipitate was generated at 3 weeks |
| Example 6 | Almost no precipitate is generated |
| Example 7 | Almost no precipitate is generated |
| Example 8 | Almost no precipitate is generated |
| Example 9 | More precipitate was generated at 2 weeks |
As can be seen from table 1, almost no precipitation was generated after the heat conductive components of example 2 and example 5 were left to stand for 2 weeks, and the dispersion stability of the heat conductive components of comparative example 2, example 3 and example 4 was superior to that of example 3 and example 4, which indicates that the heat conductive components were prepared by using oxyfluorated modified graphene oxide as a heat conductive filler, and the dispersion stability of the heat conductive components was improved. Comparative example 2 and examples 6-8, examples 6-8 showed better dispersion stability of the thermally conductive component than example 2, indicating that the addition of erythritol tetraacetate to the thermally conductive component further improved the dispersion stability of the thermally conductive component.
[ PCR thin-walled tube Performance test ]
Test specimen: the PCR thin-walled tubes in examples 10 to 17 were designated A1 to A8, respectively.
(1) Thermal conductivity testing
Adopting a DRL-III heat conductivity coefficient tester, and carrying out heat conductivity test on the surface of the PCR thin-wall tube by a steady-state heat flow method; the untreated PCR thin-walled tube was used as a blank (designated A0).
FIG. 3 shows the thermal conductivity of PCR thin walled tubes. As can be seen from FIG. 3, the thermal conductivity of the PCR thin-walled tube in examples 10-13 was higher than 0.85W/mK, whereas the thermal conductivities of the PCR thin-walled tubes in examples 10 and 13 were higher than 1.9W/mK, which is much higher than that of the blank group; comparing the thermal conductivity of the PCR thin-wall tube in the embodiment 10 with that of the embodiment 11 and the embodiment 12, the thermal conductivity of the PCR thin-wall tube in the embodiment 10 is higher than that of the embodiment 11 and the embodiment 12, which shows that the graphene oxide is modified by oxyfluoride esterification and used as a thermal conductive filler to prepare a thermal conductive component, and the PCR thin-wall tube is treated to form a layer of coating on the surface, so that the PCR thin-wall tube has higher thermal conductivity, namely, the PCR thin-wall tube can better sense the change of the external temperature and reach the target temperature, and then the liquid in the tube can be quickly heated to the target temperature.
In addition, as can be seen from fig. 3, the thermal conductivity of the PCR thin-walled tube in examples 14-16 is higher than 2.3W/m·k, the thermal conductivity of the PCR thin-walled tube in comparative examples 10 and 14-16, and examples 11 and 17, the thermal conductivity of the PCR thin-walled tube in examples 10-14 is higher than that of example 10, and the thermal conductivity of the PCR thin-walled tube in example 17 is higher than that of example 11, which indicates that the addition of erythritol tetraacetate to the heat conducting component may improve the dispersibility of the heat conducting component, so that it can be uniformly coated on the surface of the PCR thin-walled tube to form a temperature sensitive layer, which improves the thermal conductivity of the PCR thin-walled tube, and the PCR thin-walled tube has more excellent temperature sensitive performance.
(2) Water contact angle test
And placing the prepared temperature-sensitive PCR thin-wall tube on an optical contact angle measuring instrument at 25 ℃ to test the contact angle of the surface of the thin-wall tube, wherein the test liquid is deionized water, 4 mu L of each drop is dripped on the surface of the tube, and 8 different points are tested for each sample, and the average value is obtained.
FIG. 4 shows the water contact angle of PCR thin walled tubes. As can be seen from FIG. 4, the water contact angle of the PCR thin-walled tube in examples 10-13 is higher than 115 DEG, and the water contact angle of the PCR thin-walled tube in example 10 and the PCR thin-walled tube in example 13 is higher than 130 DEG, which is far higher than that of the blank group; comparing the examples 10, 11 and 12, the water contact angle of the PCR thin-walled tube in the example 10 is higher than that of the examples 11, 12, which shows that the graphene oxide modified by oxyfluorate is used as a heat conducting filler to prepare a heat conducting component, and the PCR thin-walled tube is treated to form a layer of coating on the surface, so that the water contact angle of the PCR thin-walled tube is improved, and the PCR thin-walled tube has excellent waterproof and antifouling properties. As can be seen from fig. 4, the water contact angle of the PCR thin-walled tube in examples 14-16 is higher than 135 °, and the water contact angle of the PCR thin-walled tube in examples 10-14 is higher than that of example 10, and the water contact angle of the PCR thin-walled tube in examples 11-16 and 17 is higher than that of example 11, which indicates that erythritol tetraacetate is added into the heat-conducting component, so that the dispersibility of the heat-conducting component is improved, the heat-conducting component can be uniformly coated on the surface of the PCR thin-walled tube to form a temperature sensitive layer, the waterproof and antifouling properties of the PCR thin-walled tube are better improved, the adhesion of stains is avoided, and a better cleaning effect is achieved.
(3) Coating adhesion test
According to GB9212 standard, the adhesive force of the coating on the PCR thin-wall tube is tested by a drawing method, the adhesive force grade of the coating, namely, grade 0-5, is judged according to the falling condition of the coating, and the higher the grade, the worse the adhesive force, and otherwise, the better the adhesive force.
TABLE 2 adhesion rating of coating on PCR thin walled tubes
| Sample preparation | Adhesion rating | |
| Group A1 | Level 1 | |
| | Level | 2 |
| | Level | 2 |
| Group A4 | Level 1 | |
| Group A5 | Level 1 | |
| A6 group | Level 1 | |
| Group A7 | Level 1 | |
| Group A8 | Level 1 |
As can be seen from table 2, the coating adhesion grade of the PCR thin-walled tube in example 10 and example 13 is 1 grade, which is superior to example 11 and example 12, which shows that the thermal conductive component is prepared by using the oxyfluorate-esterified modified graphene oxide as the thermal conductive filler, and the adhesion of the thermal conductive component to the PCR thin-walled tube is improved. Comparing example 11 with example 17, the coating adhesion of the PCR thin-walled tube of example 17 is better than that of example 11, which shows to some extent that the addition of erythritol tetraacetate to the thermally conductive component also improves the adhesion of the thermally conductive component to the PCR thin-walled tube.
(4) Bioerosion resistance test
The PCR thin-walled tube was placed in a culture solution (medium composition: 1.4g/L Na) inoculated with 5% sulfur oxidizing bacteria 2 HPO 4 、 1.5g/L KH 2 PO 4 、0.12g/L (NH 4 ) 2 SO 4 、0.02g/L CaCl 2 、0.02g/L FeCl 3 、0.15g/L MgSO 4 、0.025 g/L MnSO 4 、8g/L Na 2 S 2 O 3 ) And then testing the heat conductivity of the PCR thin-wall tube according to the heat conducting property test (1) in the process of [ PCR thin-wall tube performance test ], and further calculating the change rate of the heat conductivity, wherein the change rate is calculated according to the following formula:
c (%) = (thermal conductivity before treatment-thermal conductivity after treatment)/thermal conductivity before treatment x 100%
FIG. 5 shows the rate of change of thermal conductivity of PCR thin walled tubes. As can be seen from fig. 5, the thermal conductivity change rate of the PCR thin-walled tubes in example 10 and example 13 was less than 1%; comparing the thermal conductivity change rate of the PCR thin-walled tube in the embodiment 10 with the thermal conductivity change rate of the PCR thin-walled tube in the embodiment 11 with the thermal conductivity change rate of the PCR thin-walled tube in the embodiment 12, the thermal conductivity change rate is lower than that of the PCR thin-walled tube in the embodiment 11, and the thermal conductivity change rate of the PCR thin-walled tube is lower than that of the PCR thin-walled tube by using the oxyfluoride esterification modified graphene oxide as a thermal conductive filler. The thermal conductivity change rate of the PCR thin-walled tube in examples 14-16 is lower than 0.65%, the thermal conductivity change rate of the PCR thin-walled tube in examples 10-14 is lower than that of example 10, and the thermal conductivity change rate of the PCR thin-walled tube in examples 11-17 is lower than that of example 10, which indicates that erythritol tetraacetate is added into the heat conducting component, so that the dispersibility of the heat conducting component is improved, the heat conducting component can be uniformly coated on the surface of the PCR thin-walled tube to form a temperature sensitive layer, and the biological corrosion resistance of the PCR thin-walled tube is better improved.
The foregoing description is only illustrative of the preferred embodiments of the present invention, and various other changes and modifications can be made by one skilled in the art in light of the present teachings, and all such changes and modifications are intended to be included within the scope of the present teachings as defined in the appended claims.
Claims (5)
1. A temperature-sensitive PCR thin-wall tube is characterized in that: the surface of the PCR thin-wall tube is coated with a temperature sensitive coating formed by a heat conducting component; wherein, the liquid crystal display device comprises a liquid crystal display device,
the heat conducting component comprises a component a and a component b;
the component a comprises a thermally conductive filler;
the heat conducting filler is modified graphene oxide;
the modified graphene oxide is prepared by using oxyfluoride to esterify the modified graphene oxide;
the weight ratio of the graphene oxide to the oxyfluoride acid is 1:4-10;
the heat-conducting component b comprises polysiloxane and silane coupling agent;
the weight ratio of polysiloxane to silane coupling agent in the component b is 30-50:0.5-2;
the weight ratio of the component a to the component b is 2-5:10-20;
the thermal conductivity of the PCR thin-wall tube is higher than 0.85W/m.k.
2. The temperature-sensitive PCR thin-walled tube of claim 1, wherein: the preparation method of the modified graphene oxide comprises the following steps:
adding graphene oxide into an aqueous solution to uniformly disperse to obtain a dispersion liquid with the concentration of 0.4-0.8 mg/mL, dispersing the dispersion liquid into N, N-dimethylformamide to obtain a graphene oxide dispersion liquid with the concentration of 0.2-0.5 mg/mL, adding oxyfluoric acid and a catalyst into the graphene oxide dispersion liquid, performing ultrasonic dispersion, reacting for 10-16 h at the temperature of 55-75 ℃, washing, removing unreacted substances and the catalyst, and drying to obtain the modified graphene oxide.
3. The temperature-sensitive PCR thin-walled tube of claim 2, wherein: the dosage of the catalyst is 0.75-2.5% of the weight of the graphene oxide.
4. The temperature-sensitive PCR thin-walled tube of claim 1, wherein: the weight ratio of the component a to the component b is 2-4:15-20.
5. The temperature-sensitive PCR thin-walled tube of claim 1, wherein: the water contact angle of the PCR thin-walled tube is higher than 115 degrees.
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| CN109054589A (en) * | 2018-08-10 | 2018-12-21 | 四川大仁新创科技有限公司 | A kind of radiator that graphene heat conducting coating is modified |
| CN212655791U (en) * | 2020-04-07 | 2021-03-05 | 中国农业大学 | Double-temperature-control overspeed PCR amplification and real-time fluorescent signal monitoring device and portable rapid detection device |
| CN113150599A (en) * | 2021-03-26 | 2021-07-23 | 杭州安誉科技有限公司 | High-thermal-conductivity PCR reaction tube and preparation process thereof |
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| WO2019070296A1 (en) * | 2017-10-06 | 2019-04-11 | Hewlett-Packard Development Company, L.P. | Radio-frequency absorption in electronic devices |
| CN109692698A (en) * | 2018-12-29 | 2019-04-30 | 陕西师范大学 | A kind of Bi/Ti of catalytic reduction of NOx3C2Nano-sheet photochemical catalyst and preparation method thereof |
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