CN115806922B - 运动发酵单胞菌的基因工程菌株及其应用 - Google Patents
运动发酵单胞菌的基因工程菌株及其应用 Download PDFInfo
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- C12N2800/10—Plasmid DNA
- C12N2800/101—Plasmid DNA for bacteria
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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Abstract
Description
试剂 | 体积 |
US-F-primer(10μM) | 2.4μL |
DS-R-primer(10μM) | 2.4μL |
2×PrimerSTAR(Takara) | 30μL |
US fragment | 80ng |
DS fragment | 80ng |
Cat fragment | 80ng |
ddH2O | Up to 60μL |
Total volume | 60μL |
试剂 | 体积 |
DNA fragment | 0.12pM |
Vector | 0.04pM |
10×Buffer 4(Thermo) | 0.5μL |
T5 Exonuclease | 0.5U |
ddH2O | To 5μL |
试剂 | 体积 |
F-primer(10μM) | 0.4μL |
R-primer(10μM) | 0.4μL |
2×T5 Super PCR Mix(Tsingke) | 5μL |
Template(单菌落溶于10μLddH2O) | 1μL |
ddH2O | 3.2μL |
Total volume | 10μL |
温度 | 时间 | 循环数 |
98℃ | 3min | |
98℃ | 10s | 29 |
55℃ | 10s | |
72℃ | 10s | |
72℃ | 3min | |
16℃ | 保持 |
Claims (7)
Priority Applications (2)
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CN202211138020.6A CN115806922B (zh) | 2022-09-19 | 2022-09-19 | 运动发酵单胞菌的基因工程菌株及其应用 |
US18/468,813 US20240093247A1 (en) | 2022-09-19 | 2023-09-18 | Genetically engineered strain of zymomonas mobilis and uses thereof |
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CN202211138020.6A CN115806922B (zh) | 2022-09-19 | 2022-09-19 | 运动发酵单胞菌的基因工程菌株及其应用 |
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CN (1) | CN115806922B (zh) |
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CN116426448B (zh) * | 2023-03-20 | 2023-11-17 | 湖北大学 | 可视化运动发酵单胞菌、构建方法及应用 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110358767A (zh) * | 2019-07-30 | 2019-10-22 | 湖北大学 | 一种基于CRISPR-Cas12a***的运动发酵单胞菌基因组编辑方法及其应用 |
CN110358768A (zh) * | 2019-07-30 | 2019-10-22 | 湖北大学 | 一种基于运动发酵单胞菌內源CRISPR-Cas***的基因组编辑方法及其应用 |
CN110408642A (zh) * | 2019-07-30 | 2019-11-05 | 湖北大学 | 基于运动发酵单胞菌內源CRISPR-Cas***的基因组大片段高效删除方法及其应用 |
CN111849978A (zh) * | 2020-06-11 | 2020-10-30 | 中山大学 | 一种基于Type I-F CRISPR/Cas的染色质成像方法和染色质成像*** |
WO2021110993A1 (en) * | 2019-12-04 | 2021-06-10 | Synbionik Gmbh | An efficient shuttle vector system for the expression of heterologous and homologous proteins for the genus zymomonas |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
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US7354755B2 (en) * | 2000-05-01 | 2008-04-08 | Midwest Research Institute | Stable zymomonas mobilis xylose and arabinose fermenting strains |
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2022
- 2022-09-19 CN CN202211138020.6A patent/CN115806922B/zh active Active
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2023
- 2023-09-18 US US18/468,813 patent/US20240093247A1/en active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110358767A (zh) * | 2019-07-30 | 2019-10-22 | 湖北大学 | 一种基于CRISPR-Cas12a***的运动发酵单胞菌基因组编辑方法及其应用 |
CN110358768A (zh) * | 2019-07-30 | 2019-10-22 | 湖北大学 | 一种基于运动发酵单胞菌內源CRISPR-Cas***的基因组编辑方法及其应用 |
CN110408642A (zh) * | 2019-07-30 | 2019-11-05 | 湖北大学 | 基于运动发酵单胞菌內源CRISPR-Cas***的基因组大片段高效删除方法及其应用 |
WO2021110993A1 (en) * | 2019-12-04 | 2021-06-10 | Synbionik Gmbh | An efficient shuttle vector system for the expression of heterologous and homologous proteins for the genus zymomonas |
CN111849978A (zh) * | 2020-06-11 | 2020-10-30 | 中山大学 | 一种基于Type I-F CRISPR/Cas的染色质成像方法和染色质成像*** |
Non-Patent Citations (1)
Title |
---|
Establishment and application of a CRISPR– Cas12a assisted genome‑editing system in Zymomonas mobilis;Wei Shen等人;Microbial Cell Factories;第1-11页 * |
Also Published As
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CN115806922A (zh) | 2023-03-17 |
US20240093247A1 (en) | 2024-03-21 |
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