CN115590795A

CN115590795A - Cosmetic composition for skin elasticity enhancement or wrinkle improvement comprising Polygonum cuspidatum root extract

Info

Publication number: CN115590795A
Application number: CN202210787821.9A
Authority: CN
Inventors: 李珉辰; 李受珍; 沈庾美; 金尚庆; 金文周
Original assignee: LG Household and Health Care Ltd
Current assignee: LG H&H Co Ltd
Priority date: 2021-07-09
Filing date: 2022-07-06
Publication date: 2023-01-13
Also published as: KR20230009801A; KR102358611B1; KR102593544B1

Abstract

The present invention relates to a cosmetic composition for skin elasticity enhancement or wrinkle improvement comprising a polygonum cuspidatum root extract, and more particularly, to a cosmetic composition for skin elasticity enhancement or wrinkle improvement comprising a polygonum cuspidatum root extract and 2,3-butanediol or deamidated collagen as active ingredients, a pharmaceutical external product composition, and a method for skin elasticity enhancement or wrinkle improvement comprising the step of administering the composition to an individual. The composition of the present invention comprising the polygonum cuspidatum root extract and 2,3-butanediol or deamidated collagen has an excellent effect of promoting the synthesis of type i procollagen hydroxy-terminal-extending peptide (PICP), and thus can be effectively used in cosmetics for improving skin wrinkles, pharmaceutical external products, and the like.

Description

Cosmetic composition for skin elasticity enhancement or wrinkle improvement comprising Polygonum cuspidatum root extract

Technical Field

The present invention relates to a cosmetic composition for skin elasticity enhancement or wrinkle improvement, which contains a polygonum cuspidatum root extract and 2,3-butanediol or deamidated collagen as active ingredients, a pharmaceutical external product composition, and a method for skin elasticity enhancement or wrinkle improvement comprising the step of administering the composition to a subject.

Background

With the increase in aging population and the increase in life expectancy, the interest in preventing skin aging, which focuses on elasticity and wrinkle improvement, is further increasing. The skin, which is an organ occupying about 16% of the body's volume, exists on the outermost side of the body and protects our body from external environments and stimuli. Such skin is more vulnerable to external stimuli than the organs in the body, and particularly, in the case of facial skin, it is more susceptible to aging than the skin of other bodies because it is directly exposed to ultraviolet rays and harmful substances.

Skin aging is classified into intrinsic aging that occurs naturally and extrinsic aging that occurs due to external factors. The main cause of intrinsic aging is genetic elements that determine the structure and changes of skin cells and tissues. On the other hand, the factors of extrinsic aging are caused by the outside, may be the influence caused by insufficient nutrition, ultraviolet rays, environmental pollution, etc., and unlike the genetic factors, they can be completely prevented and avoided.

Such factors cause skin aging, leading to dry and rough skin, formation of wrinkles, decreased elasticity, pigmentation, and other symptoms. In particular, in the case of wrinkles and decreased elasticity, which are mentioned as representative symptoms of skin aging, there is a direct relationship with the decrease and deformation of collagen and elastin, which are major proteins in the dermis. That is, many research reports show that: skin wrinkles begin with aging of the skin, the structure of elastic fibers, i.e., elastin, changes, causing skin relaxation and wrinkles, and the activity of MMPs (matrix metalloproteinases) that bind to tissue components, such as collagen, by decomposing dermis, increases, collagen synthesis is hindered, and wrinkles increase.

The decrease in the amount of collagen is caused by the decrease in physiological functions of the skin, and the decrease in the structure and synthesis amount of collagen is accelerated by external factors such as ultraviolet rays, smoking, stress, and the like. In particular, in the case of middle-aged women, collagen disappears more rapidly after amenorrhea, aging such as decrease in skin elasticity, deepening of wrinkles, and the like is accelerated, and thus, a cosmetic composition that promotes collagen synthesis is continuously studied.

Retinoic acid, α -tocopherol, ascorbic acid, and the like are used in combination with cosmetics and pharmaceuticals to promote collagen synthesis, but when incorporated into a dosage form, discoloration or the like occurs due to instability of the raw materials, which affects the stability of the dosage form, and further, the problem of skin irritation continues to occur. In order to overcome such disadvantages, development of cosmetic compositions for wrinkle improvement using natural substances has been carried out, and development of cosmetics capable of more effectively improving and preventing skin aging has been demanded.

Polygonum Cuspidatum (Polygonum Cuspidatum) is a perennial herb belonging to the Polygonaceae family, distributed in Korea, japan, and China, growing in mountainous regions, with roots growing horizontally and sprouting to form plants and leaves growing in a staggered manner, belonging to the oval plant, and has been used as a drug for treatment of jaundice, dysentery, urination, wind, irregular menstruation, hepatitis, osteomyelitis, and the like since ancient times. The root of Polygonum cuspidatum contains polyhedrin, anthrone, chrysophanol, resveratrol, and ethanamide. The polygonum cuspidatum root extract has the effects of preventing oxidation by preventing corrosion or deterioration, preventing skin aging by blocking active oxygen to protect skin cells, preventing the activity of tyrosinase contributing to the formation of melanin to prevent the phenomenon of melanin precipitation, improving clean skin color, and the like, but the wrinkle improvement effect is less disclosed, and the efficacy synergy effect is not known.

2,3-butanediol (2, 3-butandiol) is widely used in natural fermented foods such as honey, wine and fermented soybean paste, as a natural substance widely present in natural world such as plant roots and soil, has excellent moisture-retaining effect, and also has antibacterial and antiseptic effects and crop protection effects, and thus can be used in cosmetics and agricultural fields.

Deamidated collagen (Desamido collagen) is used as a collagen that is modified in a carboxylic acid group (carboxylic acid) group and removes an amide group, and is used as an ingredient in, for example, a hair conditioner, a skin conditioner, a moisturizer, a moisture evaporation inhibitor, a skin softener, a skin membrane-forming agent, and the like, and has a moisturizing effect on the skin and provides elasticity and smoothness to the skin.

Under such circumstances, the present inventors have made extensive efforts to develop a material having a synergistic effect on the improvement of skin wrinkles using a naturally occurring material, and as a result, have confirmed that a mixed composition comprising a polygonum cuspidatum root extract and 2,3-butanediol or deamidated collagen exhibits more excellent efficacy on the improvement of wrinkles by the promotion of collagen synthesis than a case where the mixed composition is comprised of separate components, and also exhibits stability in terms of dosage form, thereby completing the present invention.

Disclosure of Invention

Problems to be solved by the invention

An object of the present invention is to provide a cosmetic composition for skin elasticity enhancement or wrinkle improvement, which contains a Polygonum Cuspidatum root extract (Polygonum Cuspidatum root extracts) as an active ingredient.

Another object of the present invention is to provide a use of a composition comprising a polygonum Cuspidatum root extract (Polygonum Cuspidatum root extracts) as an active ingredient in the manufacture of a cosmetic for enhancing skin elasticity or improving wrinkles.

Another object of the present invention is to provide a pharmaceutical external preparation composition for skin elasticity enhancement or wrinkle improvement, comprising an extract of polygonum cuspidatum root as an active ingredient.

Still another object of the present invention is to provide a use of a composition comprising an extract of polygonum cuspidatum root as an active ingredient for manufacturing a medical external product for skin elasticity enhancement or wrinkle improvement.

It is still another object of the present invention to provide a method for enhancing skin elasticity or improving wrinkles, comprising the step of administering a composition comprising an extract of polygonum cuspidatum root and 2, 3-butylene glycol or deamidated collagen as active ingredients to an individual other than a human.

Means for solving the problems

This will be described in detail below. The description and embodiments disclosed in the present invention can be applied to other descriptions and embodiments. That is, all combinations of the various elements disclosed in the present invention are included in the scope of the present invention. The scope of the present invention is not limited to the specific description below.

In order to achieve the above object, one aspect of the present invention provides a cosmetic composition for skin elasticity enhancement or wrinkle improvement, comprising a Polygonum Cuspidatum root extract (Polygonum Cuspidatum root extracts) as an active ingredient.

The cosmetic composition of the present invention may be a composition in which the polygonum cuspidatum root extract and 2, 3-butylene glycol or deamidated collagen are mixed, thereby being capable of bringing a synergistic effect on the improvement of wrinkles.

The term "giant knotweed" in the present invention is obtained by sun-drying roots and rhizomes of giant knotweed belonging to the family Polygonaceae (Polygonaceae), such as giant knotweed (Polygonum cuspidatum Sieb. Et Zucc.), giant knotweed (Polygonum sachalinense F. Schmidt ex Maxim.), polygonum ovale (Polygonum major), etc., which are perennial plants, and giant knotweed has been used as a diuretic, dysmenorrhea agent, tranquilizer since ancient times, and has been used in the oriental traditional medicine represented by China for the treatment of various diseases such as constipation, diuresis, dysmenorrhea, purulent dermatitis, urethritis, cystitis, hypertension, cancer arteriosclerosis, etc.

In the present invention, as the above-mentioned giant knotweed rhizome, a commercially available giant knotweed rhizome root is purchased and used, or a giant knotweed rhizome which is harvested or cultivated in nature is used, but not limited thereto.

The term "extract" in the present invention refers to a liquid component obtained by immersing a target substance in various solvents and then extracting the resultant product for a certain period of time at normal temperature, low temperature, or heated state, or a solid powder obtained by removing the solvent from the liquid component. In addition, the above-described results should be broadly interpreted as including dilutions of the above-described results, concentrates thereof, regulators thereof, purifications thereof, and the like. Thus, the polygonum cuspidatum root extract provided by the present invention includes the extract itself such as the extract obtained by extracting the polygonum cuspidatum root, the diluted solution or concentrated solution of the extract, the dried product obtained by drying the extract, the preparation or purified product of the extract, or the mixture thereof, and the extract of any formulation using the extract.

The method for extracting the polygonum cuspidatum root extract of the present invention is not particularly limited, and the extract can be extracted according to a method generally used in the art. Non-limiting examples of the extraction method include a hot water extraction method, an ultrasonic extraction method, a filtration method, and a loop extraction method, and these methods may be used alone or in combination of 2 or more methods.

The type of the solvent used in the extraction is not particularly limited, and any solvent known in the art may be used. As non-limiting examples of the above-mentioned extraction solvent, water, alcohol, or a mixed solvent thereof may be mentioned, and these may be used alone or in combination of one or more, specifically, alcohol may be used, and alcohol having a carbon number of 1 to 4 may be mainly used.

The extract may be used in the form of dried powder after extraction, but is not limited thereto.

The term "elasticity-enhancing" or "skin elasticity-enhancing" in the present invention means that the elasticity of the skin is improved by the composition of the present invention.

The term "wrinkle improvement" or "skin wrinkle improvement" in the present invention means that wrinkles existing in the skin of an individual are alleviated by the composition of the present invention.

The term "2, 3-butanediol" in the present invention includes 2r, 3s-butanediol and at least one of 2r, 3r-butanediol and 2s, 3s-butanediol, and is a natural substance widely present in the natural world, and it can be used in a suitable combination depending on the form and use of the cosmetic composition as a functional preparation having an excellent moisturizing effect. The 2,3-butanediol is a raw material having less cytotoxicity than other antisepsis, moisture retention and solvents such as pentanediol and 1, 2-hexanediol.

The term "deamidated collagen" in the present invention includes an amino acid sequence portion that is particularly abundant in type 3 collagen compared with other amino acids as a polypeptide (e.g., polypeptide-69) including a sequence of type 3 collagen known as a cosmetic component. In particular, amino acids that may contain a higher proportion of Proline (Proline) and Glycine (Glycine). When the deamidated collagen is applied to a cosmetic, it can be used as a single raw material or a mixed raw material. For example, infant collagen is a commonly used raw material for deamidated collagen mixtures in cosmetics ^TM Elastin (Elastin) PG20 00,

and the like.

In one example of the present invention, it was confirmed that the composition comprising the polygonum cuspidatum root extract and 2,3-butanediol or the polygonum cuspidatum root extract and deamidated collagen, respectively, could further increase the synergy exhibited by the collagen synthesis genetic gene, i.e., type i procollagen hydroxy-terminal-extending peptide (PICP), compared to the composition comprising the polygonum cuspidatum root extract, 2,3-butanediol or deamidated collagen, respectively (fig. 1 and 2).

In another example, the polygonum cuspidatum root extract is used together with 2, 3-butylene glycol to improve stability within the dosage form of the active ingredient, so as to be non-irritating and stable when applied to cosmetics (fig. 4).

The above-mentioned polygonum cuspidatum root extract and 2, 3-butylene glycol or deamidated collagen are not limited in the mixing ratio as long as the effect can be produced by mixing the polygonum cuspidatum root extract and 2, 3-butylene glycol or deamidated collagen.

For example, the extract of polygonum cuspidatum root and 2,3-butanediol can be mixed in a ratio of 1:100 to 1: 1. 1:100 to 1: 5. 1:100 to 1:10 or 1:100 to 1:20, but is not particularly limited thereto.

The mixed sample containing the above-mentioned polygonum cuspidatum root extract and 2,3-butanediol in an amount of 0.0001 to 50 wt% (v/v) based on the total weight of the composition may specifically contain 0.001 to 10 wt% (v/v), but is not particularly limited thereto.

The mixed sample containing the above Polygonum cuspidatum root extract and deamidated collagen in an amount of 0.0001 to 50 wt% (v/v) based on the total weight of the composition may specifically contain 0.001 to 5 wt% (v/v), but is not particularly limited thereto.

In the present invention, when the content of the polygonum cuspidatum root extract and 2, 3-butylene glycol or a mixed sample of the polygonum cuspidatum root extract and deamidated collagen is less than 0.0001 wt% relative to the total composition weight, the originally desired activity cannot be sufficiently achieved, and therefore, it is not preferable, and when it exceeds 15 wt%, the effect cannot be significantly improved according to the increased content, and therefore, it is not economical.

The cosmetic composition may be manufactured in a form selected from the group consisting of a solution, a topical ointment, a cream, a foam, a nourishing lotion, a softening lotion, a mask, a smoothing lotion, a foundation, essence, a soap, a liquid detergent, a body wash, a sunscreen cream, a sunscreen oil, a suspension, an emulsion, a paste, a jelly, a skin lotion, a powder, a soap, a surfactant-containing cleanser, an oil, a powder foundation cream, an emulsion foundation cream, a wax foundation cream, a patch spray, but is not limited thereto.

The cosmetic composition of the present invention may further include one or more substances selected from the group consisting of oil, water, surfactants, moisturizers, alcohols, thickeners, chelating agents, pigments, preservatives and perfumes, but is not limited thereto.

The acceptable carrier in the cosmetic field included in the cosmetic composition of the present invention varies depending on the dosage form.

When the formulation of the present invention is an ointment, a paste, a cream or a gel, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide or a mixture thereof may be used as a carrier component.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, or a mixture thereof can be used as a carrier component, and particularly, when the formulation is a spray, a propellant such as chlorofluorocarbon, propane/butane, or dimethyl ether can be contained.

In the case where the dosage form of the present invention is a solution or emulsion, as a carrier ingredient, a solvent, a solubilizer or an emulsifier may be used, and for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3-butyl glycol oil, and in particular, cottonseed oil, peanut oil, corn seed oil, olive oil, castor oil, and sesame oil, aliphatic esters of glycerin, polyethylene glycol or fatty acid esters of sorbitan may be used.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester or polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, and the like can be used.

On the other hand, when the formulation of the present invention is a capsule, the formulation may be in the form of an alginate (alginate) capsule, an agar (agar) capsule, a gelatin (gelatin) capsule, a wax (wax) capsule, or a double capsule, but is not particularly limited thereto.

The cosmetic composition of the present invention may further contain commonly used adjuvants such as a hydrophilic or lipophilic gelling agent, a hydrophilic or lipophilic active agent, a preservative, an antioxidant, a solvent, an aromatic agent, a filler, a blocking agent, a pigment, a deodorant, a dye, and the like, in addition to the giant knotweed root extract and 2,3-butanediol or the giant knotweed root extract and the deamidated collagen.

In order to achieve the above object, another aspect of the present invention provides use of a composition comprising a Polygonum Cuspidatum root extract (Polygonum Cuspidatum root extracts) as an active ingredient in the manufacture of a cosmetic composition for enhancing skin elasticity or improving wrinkles.

In order to achieve the above objects, another aspect of the present invention provides a pharmaceutical external preparation composition for skin elasticity enhancement or wrinkle improvement, comprising an extract of polygonum cuspidatum root as an active ingredient.

The medical external product composition is a composition prepared by mixing the giant knotweed rhizome extract and 2,3-butanediol or deamidated collagen.

The terms "polygonum cuspidatum root extract", "2, 3-butylene glycol", "deamidated collagen", "skin elasticity enhancement" and "wrinkle improvement" used in the present invention are as described above.

In addition to the above components, the pharmaceutical external product composition of the present invention may further comprise pharmaceutically acceptable carriers, excipients or diluents as necessary. The pharmaceutically acceptable carrier, excipient or diluent is not limited as long as the effect of the present invention is not impaired, and examples thereof include fillers, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, sweeteners, flavoring agents, preservatives, and the like.

The pharmaceutical external preparation composition of the present invention may be exemplified by disinfectant cleaners, bath lotions, ointment liquids, wet tissues, coating agents, and the like, but is not limited thereto, and the method of preparation, volume, method of use, constituent components, and the like of the pharmaceutical external preparation can be appropriately selected from techniques known in the art.

In order to achieve the above objects, in another aspect, the present invention provides a use of a composition comprising an extract of polygonum cuspidatum root as an active ingredient for manufacturing a pharmaceutical external product for skin elasticity enhancement or wrinkle improvement.

In order to achieve the above objects, according to still another aspect of the present invention, there is provided a method for enhancing skin elasticity or improving wrinkles, comprising the step of administering a composition comprising an extract of polygonum cuspidatum root to a subject.

The composition is prepared by mixing rhizoma Polygoni Cuspidati extract with 2,3-butanediol or deamidated collagen.

The terms "polygonum cuspidatum root extract", "2, 3-butanediol", "deamidated collagen", "skin elasticity enhancement" and "wrinkle improvement" used in the present invention are as described above.

The term "administering" as used herein means introducing the composition of the present invention into a patient by any suitable method, and the route of administration of the composition may be any general route that can reach the target tissue. The route of administration of the composition can be performed by applying the composition to the skin based on the property of the composition of the present invention that it is effective in enhancing skin elasticity or improving wrinkles.

The composition of the present invention may be administered daily or intermittently, and the number of administrations per day may be divided into 1 or 2 to 3. In addition, the composition of the present invention can be used alone or in combination with other drugs for the purpose of skin elasticity enhancement or wrinkle improvement. In view of all the above, it is preferable to add an amount that achieves the maximum effect with the minimum amount in the case where there is no side effect, and the amount to be added can be easily determined by those skilled in the art.

The term "individual" as used herein means any animal including mice, chevrons, and domestic animals, including humans, in which wrinkles or skin wrinkles occur or may occur. Specifically, mammals including humans can be used.

Effects of the invention

The composition for enhancing skin elasticity or improving wrinkles, which comprises the polygonum cuspidatum root extract and 2,3-butanediol or deamidated collagen as active ingredients, is safe to the skin and has a synergistic effect in promoting collagen synthesis, and therefore, can be used in cosmetics, external pharmaceutical products, and the like for enhancing skin elasticity or improving wrinkles.

Drawings

FIGS. 1A) to C) are graphs showing the expression levels of Collagen type (Collagen type) 1 in human fibroblasts when treated with Polygonum cuspidatum root extract, 2,3-butanediol, and deamidated Collagen, respectively.

FIGS. 2A) and B) are graphs showing the expression levels of Collagen type (Collagen type) 1 when a sample mixture of a Polygonum cuspidatum root extract, 2,3-butanediol, and a Polygonum cuspidatum root extract and deamidated Collagen were treated separately in human fibroblasts.

Fig. 3 a) to C) are graphs showing the expression level of Collagen type (Collagen type) 1 when a mixed sample of a polygonum cuspidatum root extract and a polyol having excellent compatibility with the polygonum cuspidatum root extract is treated in human fibroblasts.

Fig. 4 is a photograph for evaluating compatibility between 7 kinds of polyols used in cosmetics and a polygonum cuspidatum root extract.

Detailed Description

The present invention will be described in more detail below with reference to examples. These examples are intended to illustrate the present invention more specifically, and the scope of the present invention is not limited to these examples.

Example 1: culture of human fibroblasts

Fibroblasts from human foreskin (forekin) were seeded into the bottom of the culture dish, then put in DMEM medium (Dulbecco's modified eagles medium) (DMEM, gibcoBRL, u.s.a.) containing penicillin (100 IU/ml), streptomycin (100 ug/ml), and 10% fetal bovine serum (total bovine serum; FBS, gibcoBRL, u.s.a.) and cultured in an incubator (New Brunswick Scientific, UK) containing 5% carbon dioxide at 37 ℃. When the surface of the culture dish was covered with fibroblasts to an extent of about 70 to 80%, the cells were collected by treating with 0.25% trypsin (Gibco BRL, u.s.a.) and cultured, and a part of the cells were stored frozen in liquid nitrogen for subculture.

Example 2: evaluation of enhancement of collagen Synthesis

The collagen synthesis enhancing effect was evaluated at the cellular level by adding the polygonum cuspidatum root extract, 2,3-butanediol, deamidated collagen, separately or in combination to the culture medium of human-derived fibroblasts. The type i procollagen hydroxy-terminal-extended peptide (PICP), which is typically most synthesized in fibroblasts, was quantitatively determined at the time of evaluation.

Specifically, human fibroblasts were treated at 1x10, respectively ⁵ The/well was aliquoted into cell culture plates (24 well cell culture plates) and cultured in DMEM containing 10% fetal bovine serum for 24 hours. After the cells were replaced with DMEM containing no serum, each sample was diluted with a culture medium and treated for 48 hours. 20ul of the culture supernatant was removed and the degree of collagen increase at each concentration was measured at 450nm using a PICPEIA KIT (Procollagen type I C-Peptide Enzyme linked immunosorbent assay KIT). Quantitative measurements were performed by plotting PICP standard concentrations, and experiments were performed in 3 cases for each concentration, and the results were averaged and shown. As a positive control group, TGF-. Beta.s (10 ng/ml) were used.

As a result, as shown in fig. 1, when the polygonum cuspidatum root extract, 2,3-butanediol, and deamidated Collagen were treated alone, the expression of Collagen type (Collagen type) 1 was increased, which was a result of satisfying the known effects of the respective raw materials.

However, it was confirmed in fig. 2 that the collagen expression level was significantly increased in the case of the treatment in which the polygonum cuspidatum root extract and 2,3-butanediol or the polygonum cuspidatum root extract and deamidated collagen were mixed, as compared with the case of the single treatment in fig. 1.

Thus, the cosmetic composition of the present invention exhibits an effect of enhancing skin elasticity by increasing the expression level of collagen, and as to the effect, when the polygonum cuspidatum root extract and 2,3-butanediol or deamidated collagen are mixed, the effect is more enhanced than when each is treated separately.

Example 3: compatibility comparison of Polygonum cuspidatum root extract and polyol

In order to evaluate the degree of precipitation or stabilization of the polygonum cuspidatum root extract, the compatibility with 7 types of polyols used in cosmetic materials was evaluated.

Specifically, examples 1 and 2 were prepared by adding 0.5% or 5.0% of the polygonum cuspidatum root extract to 2,3-butanediol and stirring at 900rpm for 1 hour at room temperature.

In addition, comparative examples 1 to 12 were produced in the same manner as in example 1, except that the polyol was different. The difference in stability of the polygonum cuspidatum root extract tends to be more pronounced at low temperatures than at high temperatures or temperature cycling conditions, and therefore the compatibility of examples 1 and 2 and comparative examples 1 to 12 was confirmed after storage for 4 weeks under normal temperature (25 ℃) conditions after stirring. In the case of freezing (-20 ℃ C.), thawing and freezing were repeated 5 times during the period. The stability under each condition was visually observed with emphasis on precipitation and discoloration and shown in tables 1 and 2 below.

TABLE 1

Evaluation criteria-O: stable, Δ: micro-precipitation, X: precipitation out of

TABLE 2

Evaluation criteria-O: stable, Δ: micro-precipitation, X: precipitation out

As a result, as shown in tables 1 and 2,3-butanediol was most excellent in compatibility with the extract of alpine rush root as described in examples 1 and 2.

The excellent degree of ethoxydiglycol and dimethyl isosorbide are similar, but in the case of ethoxydiglycol, there is a problem of skin irritation when used in a small amount, and when used at a high content (comparative example 10), the color changes to pink, and the color change further deepens as the shelf life becomes longer. Dimethyl isosorbide shows a light pink color when 0.5% of the polygonum cuspidatum root extract is used (comparative example 12), and the degree of discoloration increases with increasing content of the polygonum cuspidatum root extract.

From this, it was confirmed that when the polygonum cuspidatum root extract and 2,3-butanediol were used together, the results were more excellent not only in terms of efficacy but also in terms of the compatibility and stability of the raw materials.

Example 4: evaluation of collagen Synthesis Capacity of Polygonum cuspidatum root extract and various polyols

In addition to 2,3-butanediol, 1,3-butanediol, ethoxydiglycerol, and dimethyl isosorbide, which were evaluated to have excellent compatibility with the polygonum cuspidatum root extract in table 2, were additionally evaluated and the collagen synthesis ability when mixed with the polygonum cuspidatum root extract was confirmed.

As a result, as shown in fig. 3, it was confirmed that when the polygonum cuspidatum root extract and 2,3-butanediol were mixed and used, the collagen synthesis ability was significantly enhanced, but the collagen synthesis ability was rather inhibited in the other mixed sample with polyhydric alcohol.

Production example 1: preparation of essence composition

In order to evaluate the formulation stability of the cosmetic comprising the polygonum cuspidatum root extract and 2, 3-butylene glycol or deamidated collagen, cosmetic compositions were prepared with the components and contents described in table 3 below.

Specifically, in example 3, the raw material 3 was dissolved in the raw material 1 at the composition ratio shown in table 3, and then the raw materials 4 to 10 were added and dissolved. After the dissolution of the raw material 3 was confirmed, the raw materials 4 to 9 were dissolved in this order, and then the raw material 10 was slowly added and stirred uniformly. The essences were prepared by measuring the raw materials 11 to 13 in a separate beaker, dissolving by heating to 40 to 45 ℃, and then gradually adding to the aqueous phase while stirring uniformly for 15 minutes. Comparative examples 13 and 14 were produced in the same manner as in example 3.

Essence type cosmetics (unit: wt%) of the experimental group containing 2, 3-butylene glycol and 1, 3-butylene glycol and the control group containing no butylene glycol were prepared using the components and contents shown in table 3 below.

TABLE 3

	Name of the ingredient	Example 3	Comparative example 13	Comparative example 14
					1	2,3-butanediol	10	-	-
2	1, 3-butanediol	-	10	-
					3	Extract of Polygonum cuspidatum root	0.2	0.2	0.2
4	Purified water	To100	To100	To100
					5	Chelating agents	Proper amount of	Proper amount of	Proper amount of
6	Preservative	Proper amount of	Proper amount of	Proper amount of
					7	Betaine	5	5	5
8	Trehalose	1	1	1
					9	Allantoin	0.2	0.2	0.2
10	Xanthan gum	0.2	0.2	0.2
					11	Cosolvent	0.5	0.5	0.5
12	Ethanol	3	3	3
					13	Perfume	Proper amount of	Proper amount of	Proper amount of

(Unit:% by weight)

Example 5: evaluation of formulation stability

The cosmetic compositions of example 3, comparative example 13 and comparative example 14 were evaluated for compatibility. As for the storage conditions, temperature conditions with significant differences among experiments were selected and observed again at normal and low temperatures, and for comparison, additional observation was also performed at high temperature conditions, as in the previous polyol compatibility evaluation.

Specifically, the cosmetic composition was stored in a glass container for 4 weeks at high temperature (45 ℃ C.), normal temperature (25 ℃ C.), and refrigerated storage (0 ℃ C.). In the case of freezing (-20 ℃ C.), thawing and freezing were repeated 5 times. The presence or absence of separation and precipitation of the phase (phase) was visually observed under the respective conditions, and is shown in table 4 below.

TABLE 4

	Example 3	Comparative example 13	Comparative example 14
				High temperature (45 ℃ C.)	○	○	○
Normal temperature (25 ℃ C.)	○	○	△
				Refrigeration (0 ℃ C.)	○	○	△
Freezing (-20 ℃ C.)	○	△	X

Evaluation criteria-O: stable, Δ: micro-precipitation, X: precipitation out

As a result, as shown in Table 4, in example 3 using 2,3-butanediol, no precipitation occurred under all storage conditions, and a uniform and stable formulation was maintained. However, in the case of comparative example 13 in which 1, 3-butanediol was used, slight deposition occurred during repetition of the freezing conditions, and the amount of deposition increased with the number of repetitions. In addition, in the case of comparative example 14 in which no polyol was used, precipitation was observed under most of the storage conditions.

The above experimental results confirmed that the polyhydric alcohol contributes to the stability of the formulation of the polygonum cuspidatum root extract, and the stability of the cosmetic can be significantly improved when used together with 2, 3-butylene glycol in the polyhydric alcohol.

Example 6: evaluation of skin stability

In order to confirm the skin stability of the above-mentioned substances as cosmetics, tests were carried out with respect to skin irritation.

Specifically, the subject was 20 healthy adult volunteers having no skin disease at the experimental site, and the I Q Ultra was used ^TM The patch test units (Chemotechnique MB Diagnostics AB, vellinge, sweden) performed stimulation tests. When the primary stimulation index was calculated using the following calculation formula, a sample exceeding 0.20 (in the case where a positive stimulation reaction corresponding to 1 point was observed at a frequency exceeding 20% of all the subjects or in the case where the reaction corresponds to this case) was judged as a sample that could induce stimulation, and 1% Sodiu m Lauryl Sulfate (CAS Number: 151-21-3) aqueous solution was used as a standard substance. As a result, it was confirmed that the composition was safe to the skin as shown in table 5 below.

TABLE 5

Name of sample	Stimulation index
		Positive control (Positive control) (1.0% SLS)	0.18
Example 3 ^*	0.03

* Example 3: comprises rhizoma Polygoni Cuspidati extract and 2,3-butanediol

In summary, in the case of the mixed composition comprising the polygonum cuspidatum root extract of the present invention and 2, 3-butylene glycol or deamidated collagen, the skin elasticity-enhancing or wrinkle-improving effect by promoting collagen synthesis is more excellent and the formulation is more stable than the case of including the same as the individual components.

As is apparent from the above description, those skilled in the art can implement the present invention in other specific forms without changing the technical idea or essential features thereof. In this regard, the above-described embodiments are merely exemplary in all aspects, and the present invention is not limited thereto. In the present invention, the scope of the present invention is defined by the meaning of the claims to be described later, and all modifications and variations derived from the equivalent concept are included in the scope of the present invention.

Claims

1. Use of a composition comprising an extract of the root of Polygonum cuspidatum as an active ingredient in the manufacture of a cosmetic for enhancing skin elasticity or improving wrinkles.

2. The use according to claim 1, wherein,

the composition further comprises 2, 3-butylene glycol or deamidated collagen.

3. The use according to claim 2, wherein,

the above composition promotes the synthesis of procollagen type I C-terminal propeptide (PICP) type 1.

4. The use according to claim 1, wherein,

the composition further comprises one or more selected from the group consisting of water, surfactant, humectant, alcohol, thickener, chelating agent, pigment, antiseptic and perfume.

5. Use of a composition comprising an extract of the root of Polygonum cuspidatum as an active ingredient for the manufacture of a pharmaceutical external product for enhancing skin elasticity or improving wrinkles.

6. The use according to claim 5, wherein,

the composition further comprises 2, 3-butylene glycol or deamidated collagen.