CN115568383A - Method for improving survival rate of tissue culture seedlings in temporary planting - Google Patents
Method for improving survival rate of tissue culture seedlings in temporary planting Download PDFInfo
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- CN115568383A CN115568383A CN202211244757.6A CN202211244757A CN115568383A CN 115568383 A CN115568383 A CN 115568383A CN 202211244757 A CN202211244757 A CN 202211244757A CN 115568383 A CN115568383 A CN 115568383A
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- 230000004083 survival effect Effects 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 77
- 239000000758 substrate Substances 0.000 claims abstract description 45
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 32
- 230000003385 bacteriostatic effect Effects 0.000 claims abstract description 29
- 239000002689 soil Substances 0.000 claims abstract description 28
- 206010016807 Fluid retention Diseases 0.000 claims abstract description 26
- 239000001963 growth medium Substances 0.000 claims abstract description 24
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 claims abstract description 11
- 239000005781 Fludioxonil Substances 0.000 claims abstract description 11
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 claims abstract description 11
- 229930182566 Gentamicin Natural products 0.000 claims abstract description 11
- 239000005842 Thiophanate-methyl Substances 0.000 claims abstract description 11
- MUJOIMFVNIBMKC-UHFFFAOYSA-N fludioxonil Chemical compound C=12OC(F)(F)OC2=CC=CC=1C1=CNC=C1C#N MUJOIMFVNIBMKC-UHFFFAOYSA-N 0.000 claims abstract description 11
- 229960002518 gentamicin Drugs 0.000 claims abstract description 11
- 229940069338 potassium sorbate Drugs 0.000 claims abstract description 11
- 235000010241 potassium sorbate Nutrition 0.000 claims abstract description 11
- 239000004302 potassium sorbate Substances 0.000 claims abstract description 11
- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical group COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 claims abstract description 11
- 238000005406 washing Methods 0.000 claims abstract description 11
- 238000004140 cleaning Methods 0.000 claims abstract description 6
- 238000003756 stirring Methods 0.000 claims abstract description 6
- 238000010521 absorption reaction Methods 0.000 claims description 19
- 230000000844 anti-bacterial effect Effects 0.000 claims description 11
- 238000005507 spraying Methods 0.000 claims description 9
- 235000013162 Cocos nucifera Nutrition 0.000 claims description 7
- 244000060011 Cocos nucifera Species 0.000 claims description 7
- 239000003415 peat Substances 0.000 claims description 7
- 229920002401 polyacrylamide Polymers 0.000 claims description 7
- 229920005614 potassium polyacrylate Polymers 0.000 claims description 7
- 239000007921 spray Substances 0.000 claims description 6
- 230000008961 swelling Effects 0.000 claims description 6
- 230000007480 spreading Effects 0.000 claims description 4
- 239000011159 matrix material Substances 0.000 abstract description 7
- 201000010099 disease Diseases 0.000 abstract description 4
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- 241000196324 Embryophyta Species 0.000 description 9
- 241000234671 Ananas Species 0.000 description 7
- 235000007119 Ananas comosus Nutrition 0.000 description 7
- 239000000022 bacteriostatic agent Substances 0.000 description 6
- 240000000111 Saccharum officinarum Species 0.000 description 4
- 235000007201 Saccharum officinarum Nutrition 0.000 description 4
- 238000002513 implantation Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
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- 241001646828 Platostoma chinense Species 0.000 description 2
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- 238000005457 optimization Methods 0.000 description 1
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- 239000000575 pesticide Substances 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
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- 230000014616 translation Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
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- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
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Abstract
The invention discloses a method for improving the survival rate of tissue culture seedling heeling-in, which comprises the following steps: (1) Preparing a bacteriostatic water-retention culture medium, and putting a water-retention agent into bacteriostatic water to absorb water, wherein the bacteriostatic water contains 1-5 mg/L thiophanate methyl, 1-5 mg/L fludioxonil, 1-10 mg/L potassium sorbate and 1-10 mg/L gentamicin; (2) Preparing a substrate, uniformly stirring the water-absorbed bacteriostatic water-retention culture medium and the seedling culture substrate, and then flatly paving the mixture in a plug tray; (3) Cleaning, taking out the tissue culture seedlings, washing the tissue culture seedlings clean by running water, and keeping the tissue culture seedlings moist; and (4) performing temporary planting, namely performing temporary planting on the tissue culture seedling into a plug tray. According to the invention, the water-retaining agent is added into the seedling raising matrix, so that the seedling raising matrix can automatically adjust the soil moisture, and the water-retaining agent absorbs the antibacterial agent, so that the agent can be slowly released, the effective period can be prolonged, the agent can be prevented from losing efficacy along with the moisture loss, the occurrence and the diffusion of diseases can be controlled in a longer time, and the survival rate and the growth effect of the temporary planting can be improved.
Description
Technical Field
The invention belongs to the technical field of plant seedling breeding, and particularly relates to a method for improving the survival rate of temporary planting of a tissue culture seedling.
Background
After the tissue culture seedlings cultured by the plant tissue culture technology are rooted in the bottle, the tissue culture seedlings can be moved out of the room for temporary planting. The critical period of survival of the tissue culture seedling is 5-15 days after the temporary planting of the tissue culture seedling, the tissue culture seedling is changed from a heterotrophic state to an autotrophic state, the absorption capacity of a root system is weak, the outdoor environment changes greatly, stable temperature and humidity are not easy to maintain, and once the tissue culture seedling is not managed in time, the situation that the tissue culture seedling loses water or the root system is rotted and diseased due to the influence of fungi, bacteria and high temperature and high humidity is easily caused.
Disclosure of Invention
The invention aims to provide a method for improving the survival rate of tissue culture seedling temporary planting aiming at the defects of the existing tissue culture seedling temporary planting technology.
In order to realize the purpose of the invention, the following technical scheme is adopted:
a method for improving the survival rate of tissue culture seedling heeling-in comprises the following steps:
(1) Preparing a bacteriostatic water-retention culture medium, putting a water-retention agent into bacteriostatic water for water absorption swelling, wherein the pH value of the bacteriostatic water is 5.5-7, the bacteriostatic water contains 1-5 mg/L thiophanate methyl, 1-5 mg/L fludioxonil, 1-10 mg/L potassium sorbate and 1-10 mg/L gentamicin, and the bacteriostatic water-retention culture medium can be obtained after water absorption is finished;
(2) Preparing a substrate, uniformly stirring the bacteriostatic water-retention culture medium after water absorption in the step (1) and the seedling substrate to obtain a temporary planting seedling substrate, and then spreading the temporary planting seedling substrate in a seedling raising hole tray;
(3) Cleaning, taking out the tissue culture seedling to be subjected to temporary planting from a culture bottle, washing with running water, washing the original culture medium adhered to the tissue culture seedling, and then putting the root of the tissue culture seedling into water to keep moist:
(4) And (4) temporarily planting, temporarily planting the cleaned tissue culture seedlings into a seedling raising hole tray, watering enough root fixing water, keeping out the shade, and controlling the temperature and humidity conditions.
Preferably, the water retention agent is 1 or 2 of potassium polyacrylate and polyacrylamide. The mass of the water-retaining agent accounts for 0.1-5% of the total mass of the seedling raising substrate. The water-retaining agent is a mixture of potassium polyacrylate and polyacrylamide, and the mass ratio of the potassium polyacrylate to the polyacrylamide is 1-2.
Preferably, the weighed water-retaining agent is put into bacteriostatic water with the weight 5-30 times of the weight of the bacteriostatic water to absorb water for swelling, and the water absorption time is more than or equal to 10min, and is usually within 30 min.
As the optimization of the technical proposal, the bacteriostatic water contains 1mg/L thiophanate-methyl, 2mg/L fludioxonil, 5mg/L potassium sorbate and 5mg/L gentamicin.
Preferably, the seedling substrate comprises 1-10% of peat soil, 10-50% of gravel, 1-5% of coconut coir and 30-80% of soil. The seedling culture medium consists of peat soil, gravel, coconut coir and soil, so that the seedling culture medium is loose and breathable and maintains certain nutritional ingredients.
After the temporary planting is finished, the soil humidity is kept at 70-80%, and spraying equipment is adopted to spray water and preserve moisture 5-15 days after the temporary planting according to the air humidity and the soil humidity, so that the survival rate of the temporary planted seedlings is improved.
The working principle is as follows:
when the traditional tissue culture seedling is heeled in, the sun is needed, and certain humidity is kept, so that the tissue culture seedling is prevented from wilting and dying due to water shortage. The water supplement not only keeps the seedling culture substrate moist, but also needs spraying equipment to regularly spray in the air so as to ensure the growth requirement of the tissue culture seedlings. And the conventional seedling raising substrate is kept in a wet state due to long-term water spraying, and the long-term immersion of the root system in the seedling raising substrate can cause the root system to grow badly and even die after the tissue culture seedling is planted in the seedling raising substrate. The invention adopts peat soil, gravel, coconut chaff and soil as seedling culture substrates, has trace nutrient components, ensures the looseness and air permeability of the substrates, avoids soil hardening, can keep certain moisture, promotes the growth of root systems and improves the survival rate of tissue culture seedlings. The water-retaining agent is added into the matrix, so that the soil can absorb moisture, the phenomenon of root soaking caused by excessive moisture is avoided, and meanwhile, the humidity of the seedling culture matrix is controlled within a certain range, so that the water spraying frequency can be reduced, and the workload is reduced. Secondly, thiophanate-methyl, fludioxonil, potassium sorbate and gentamicin are adsorbed by the water-retaining agent, the growth of microorganisms is inhibited in modes of interfering hyphal division, inhibiting microbial enzyme activity, destroying enzyme systems, destroying protein synthesis and the like by medicaments, and meanwhile, the pesticide effect period is long due to the fact that the thiophanate-methyl, fludioxonil, potassium sorbate and gentamicin are adsorbed in the water-retaining agent, so that the occurrence of later-stage diseases is reduced, and the diffusion of microorganisms on dead and rotten tissue culture seedlings to surrounding tissue culture seedlings is inhibited. In addition, when the tissue culture seedlings need to be transplanted, the water-retaining agent in the seedling culture substrate can provide water for the temporary planting seedlings in the early stage of transportation and transplantation, and the survival rate of the tissue culture seedling transplantation is improved.
The invention has the advantages that:
1. in the temporary planting of the tissue culture seedling, the method only needs to thoroughly drench the temporary planting seedling culture substrate with water and keep the air humidity when the tissue culture seedling is temporarily planted, the temporary planting seedling culture substrate does not need to be drenched 5-15 days after the temporary planting (according to the weather condition), the phenomenon that the excessive water drenches to cause the excessive substrate water content to influence the growth of a root system is avoided, and meanwhile, the seedling culture substrate and the water-retaining agent can provide a moist environment for the root system, so that the water absorption of the tissue culture seedling is ensured.
2. According to the invention, the water-retaining agent is added into the seedling substrate, so that the seedling substrate can automatically adjust the soil moisture, and the water-retaining agent absorbs antibacterial agents such as thiophanate methyl, fludioxonil, potassium sorbate and gentamicin, so that the pesticide can be slowly released, the drug effect period is prolonged, the drug is prevented from losing efficacy along with water loss, and the occurrence and diffusion of diseases can be controlled in a longer time.
3. The method only needs bacteriostasis control of the water-retaining agent in the early stage in the temporary planting period of the tissue culture seedling, does not need subsequent operations such as additional spraying of a bactericide and the like, can reduce the workload, avoids the influence on the tissue culture seedling caused by improper management to a certain extent, and has good effects on improving the survival rate of the temporary planting of the tissue culture seedling and reducing the occurrence of diseases.
Detailed Description
The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only used as examples, and the protection scope of the present invention is not limited thereby. It is to be noted that, unless otherwise specified, technical or scientific terms used herein shall have the ordinary meaning as understood by those skilled in the art to which the invention pertains.
Example 1
A method for improving the survival rate of tissue culture seedlings in temporary planting comprises the following steps,
(1) Preparing an antibacterial water retention culture medium, and putting a water retention agent (mass ratio of potassium polyacrylate to polyacrylamide =2: 1) into 20-30 times of antibacterial water for water absorption and swelling, wherein the water absorption time is 10-15 min. The pH value of the bacteriostatic water is 5.5-7, and the bacteriostatic water contains 1mg/L thiophanate methyl, 2mg/L fludioxonil, 5mg/L potassium sorbate and 5mg/L gentamicin; and obtaining the bacteriostatic water-retention culture medium after water absorption. The mass of the water-retaining agent accounts for 1-2% of the total mass of the seedling raising substrate.
(2) And (3) preparing a substrate, uniformly stirring the bacteriostatic water-retention culture medium after water absorption in the step (1) and the seedling substrate to obtain a temporary planting seedling substrate, and spreading the temporary planting seedling substrate in a seedling raising hole tray. The seedling raising substrate comprises 10% of peat soil, 10% of gravel, 2% of coconut coir and 78% of soil.
(3) Cleaning, taking out the tissue culture seedling to be subjected to temporary planting from the culture bottle, washing with running water, washing the original culture medium adhered to the tissue culture seedling, and then putting the root of the tissue culture seedling into water to keep moist.
(4) Temporarily planting, namely, watering the temporarily planted seedling raising matrix thoroughly before temporarily planting, then temporarily planting the cleaned tissue culture seedlings into seedling raising hole trays for planting, paying attention to shade, and controlling the conditions of temperature and humidity; after the temporary planting is finished, the soil humidity is kept at 80%, and spraying equipment is adopted to spray water for moisturizing 7 days after the temporary planting according to the air humidity and soil humidity conditions, so that the survival rate of the temporary planting seedlings is improved.
The tissue culture seedling variety of the temporary planting is sugarcane tissue culture seedlings, 30 sugarcane tissue culture seedlings are divided into 3 groups, 1 group is additionally arranged to be a control group, and the water-retaining agent in the control group absorbs common water and does not contain bacteriostatic agent. The temporary planting condition management adopts the existing temperature and humidity control method. The data from observations and statistics after 30 days of temporary implantation are tabulated below:
TABLE 1 statistics of survival rate of temporary planting of sugarcane tissue culture seedlings
From the above table 1, after 30 days of temporary planting, the survival rate of 3 groups of sugarcane tissue culture seedlings treated by the method of the invention reaches more than 90%, the seedlings grow well, while the root rot phenomenon of partial group culture seedlings of the control group occurs, the serious seedlings even the whole seedlings are in water stain shape and are softened and rotted, partial group culture seedlings can grow normally, but are inhibited in the growth process, the seedlings grow weakly, and the overall growth is uneven.
Example 2
A method for improving the survival rate of tissue culture seedlings in temporary planting comprises the following steps,
(1) Preparing an antibacterial water-retention culture medium, and putting a water-retention agent (polyacrylamide) into 10-20 times of antibacterial water for water absorption and swelling for 15-20 min. The pH value of the bacteriostatic water is 5.5-7, and the bacteriostatic water contains 1mg/L thiophanate methyl, 2mg/L fludioxonil, 5mg/L potassium sorbate and 5mg/L gentamicin; and obtaining the bacteriostatic water-retention culture medium after water absorption. The mass of the water-retaining agent accounts for 4-5% of the total mass of the seedling raising substrate.
(2) And (2) preparing a substrate, uniformly stirring the bacteriostatic water-retention culture medium after absorbing water in the step (1) and the seedling culture substrate to obtain a temporary planting seedling culture substrate, and then flatly paving the temporary planting seedling culture substrate in a seedling culture hole tray. The seedling culture substrate comprises 5% of peat soil, 15% of gravel, 5% of coconut coir and 75% of soil.
(3) Cleaning, taking out the tissue culture seedling to be subjected to temporary planting from the culture bottle, washing with running water, washing the original culture medium adhered to the tissue culture seedling, and then putting the root of the tissue culture seedling into water to keep moist.
(4) Temporarily planting, namely, watering the temporarily planted seedling raising matrix thoroughly before temporarily planting, then temporarily planting the cleaned tissue culture seedlings into seedling raising hole trays for planting, paying attention to shade, and controlling the conditions of temperature and humidity; after the temporary planting is finished, the soil humidity is kept at 70%, and 5 days after the temporary planting, spraying equipment is adopted to spray water and preserve moisture according to the conditions of air humidity and soil humidity, so that the survival rate of the temporary planting seedlings is improved.
The tissue culture seedling variety for temporary planting is pineapple tillering bud tissue culture seedling, 30 pineapple tissue culture seedlings are used as one group, 3 groups are divided, 1 group is added as a control group, and the water retention agent in the control group absorbs common water and does not contain bacteriostatic agent. The temporary planting condition management adopts the existing temperature and humidity control method. The data of the observation and statistical experiment after 30 days of temporary implantation are tabulated below:
TABLE 2 statistics of survival rate of heeling-in seedlings of pineapple tillering buds
From the above table 2, it can be seen that, after 30 days of tissue culture temporary planting of pineapple, the survival rate of the tissue culture seedlings of pineapple treated by the method is higher than that of the tissue culture seedlings of pineapple treated by the method without adding the bacteriostatic agent, but the difference is not obvious, but the growth vigor of plants is better, and the leaves at the base of the seedlings of pineapple without adding the bacteriostatic agent are partially yellowed or water-soaked, which indicates that the plants are affected by harmful microorganisms in a high-humidity environment, and further affects the size and height of the plants.
Example 3
A method for improving the survival rate of tissue culture seedlings in temporary planting comprises the following steps,
(1) Preparing an antibacterial water retention culture medium, and putting a water retention agent (potassium polyacrylate) into 25-30 times of antibacterial water for water absorption and swelling for 20-25 min. The pH value of the bacteriostatic water is 5.5-7, and the bacteriostatic water contains 2mg/L thiophanate methyl, 2mg/L fludioxonil, 10mg/L potassium sorbate and 10mg/L gentamicin; and obtaining the bacteriostatic water-retention culture medium after water absorption. The mass of the water-retaining agent accounts for 0.5-1% of the total mass of the seedling raising substrate.
(2) And (3) preparing a substrate, uniformly stirring the bacteriostatic water-retention culture medium after water absorption in the step (1) and the seedling substrate to obtain a temporary planting seedling substrate, and spreading the temporary planting seedling substrate in a seedling raising hole tray. The seedling culture substrate comprises 5% of peat soil, 20% of gravel, 5% of coconut coir and 70% of soil.
(3) Cleaning, taking out the tissue culture seedling to be heeled in from the culture bottle, washing with running water, washing the original culture medium adhered on the tissue culture seedling, putting the root of the tissue culture seedling into water to keep moist,
(4) Performing temporary planting, namely watering the temporary planting seedling raising matrix thoroughly before temporary planting, then temporarily planting the cleaned tissue culture seedlings into seedling raising hole trays for planting, paying attention to shade, and controlling the conditions of temperature and humidity; after the temporary planting is finished, the soil humidity is kept at 80%, and spraying equipment is adopted to spray water and preserve moisture in 12 days after the temporary planting according to the conditions of air humidity and soil humidity, so that the survival rate of the temporary planted seedlings is improved.
The tissue culture seedling variety of the temporary planting is mesona blume tissue culture seedling, 30 mesona blume tissue culture seedlings are used as one group, 3 groups are divided, 1 group is added to be used as a control group, and the water retention agent in the control group absorbs common water and does not contain bacteriostatic agent. The temporary planting condition management adopts the existing temperature and humidity control method. The data of the observation and statistical experiment after 30 days of temporary implantation are tabulated below:
TABLE 3 Mesona chinensis Benth tissue culture seedling heeling-in survival rate statistics
From the above table 3, after 30 days of temporary planting, the Mesona chinensis Benth grows vigorously and plants flourish, but the control plants without bacteriostatic agent are generally short and small, and some plants have wilting and rotting leaves, so the survival rate is obviously lower than that of the plants treated by the method, and the growth of the plants is inhibited more seriously.
Claims (8)
1. A method for improving the survival rate of tissue culture seedling heeling in is characterized in that: comprises the following steps of (a) carrying out,
(1) Preparing an antibacterial water-retention culture medium, putting a water-retention agent into antibacterial water for water absorption and swelling, wherein the pH value of the antibacterial water is 5.5-7, and the antibacterial water contains 1-5 mg/L thiophanate-methyl, 1-5 mg/L fludioxonil, 1-10 mg/L potassium sorbate and 1-10 mg/L gentamicin; obtaining the bacteriostatic water-retention culture medium after water absorption is finished;
(2) Preparing a substrate, uniformly stirring the bacteriostatic water-retention culture medium after water absorption in the step (1) and the seedling substrate to obtain a temporary planting seedling substrate, and then spreading the temporary planting seedling substrate in a seedling raising hole tray;
(3) Cleaning, taking out the tissue culture seedling to be subjected to temporary planting from a culture bottle, washing with running water, washing the original culture medium adhered to the tissue culture seedling, and then putting the root of the tissue culture seedling into water to keep moist;
(4) And (4) temporarily planting, temporarily planting the cleaned tissue culture seedlings into a seedling raising hole tray for planting, watering enough root fixing water, paying attention to sun shading, and controlling the temperature and humidity conditions.
2. The method for improving the survival rate of tissue culture seedling heeling in of claim 1, wherein: the water-retaining agent is 1 or 2 of potassium polyacrylate and polyacrylamide.
3. The method for improving the survival rate of tissue culture seedling heeling in according to claim 1 or 2, wherein the mass ratio of the potassium polyacrylate to the polyacrylamide in the water retaining agent is 1-2.
4. The method for improving the survival rate of tissue culture seedling heeling-in according to claim 1 or 2, wherein: the mass of the water-retaining agent accounts for 0.1-5% of the total mass of the seedling raising substrate.
5. The method for improving survival rate of tissue culture seedling heeling in according to claim 1, wherein: the weighed water-retaining agent is put into bacteriostatic water with the weight 5-30 times of the weight to absorb water and expand, and the water absorption time is more than or equal to 10min.
6. The method for improving the survival rate of tissue culture seedling heeling in of claim 1, wherein: the bacteriostatic water contains 1mg/L thiophanate methyl, 2mg/L fludioxonil, 5mg/L potassium sorbate and 5mg/L gentamicin.
7. The method for improving survival rate of tissue culture seedling heeling in according to claim 1, wherein: the seedling raising substrate comprises 1-10% of peat soil, 10-50% of gravel, 1-5% of coconut coir and 30-80% of soil.
8. The method for improving the survival rate of tissue culture seedling heeling in of claim 1, wherein: after the temporary planting is finished, the soil humidity is kept at 70-80%, and spraying equipment is adopted to spray water and keep moisture 5-15 days after the temporary planting according to the air humidity and the soil humidity, so that the survival rate of the temporary planted seedlings is improved.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211244757.6A CN115568383A (en) | 2022-10-12 | 2022-10-12 | Method for improving survival rate of tissue culture seedlings in temporary planting |
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